Sample aspiration tip

Abstract

To provide a chip for sample suction with a simple configuration capable of securely sucking even a small amount of sample no longer maintaining a uniform liquid level and capable of gently injecting the sucked sample into a sample tube without causing the sample to splash or mix with other liquid in the sample tube.SOLUTION: A chip for sample suction 100 includes: an attachment part 110 provided with an upper opening 111 that can be connected to a dispenser W for supply and exhaust; an intermediate part 120 provided with a holding part 121 for holding a sample; and a tip part 130 provided with a discharge port 133 for suction / discharge of the sample. A part or a whole of the tip part 130, following from the discharge port 133, forms an insertion part 122. The maximum outer diameter of the insertion part 122 is smaller than the inner diameter of an opening of the sample tube. The center of the discharge port 133 must be located away from a central axis B of the intermediate part 120.SELECTED DRAWING: Figure 2

Description

Technical Field

[0001] The present invention relates to a sample suction tip used by connecting to a dispensing device for dispensing a sample such as blood contained in a sample tube.

Background Art

[0002] Conventionally, as a sample suction tip used by connecting to a dispensing device when processing a sample such as blood contained in a sample tube, a sample suction tip used in the centrifugation method of a sample described in Citation 1 is known. The sample suction tip (pipette tip 4) described in this Patent Document 1 is used in a dispensing device (automatic centrifugation processing device), and is attached to the tip of a pipette 5 attached to a holder (pipette holder 12) provided above a chip lifting and lowering unit (telescopic stand 11). An air tube (air suction port 8 and air discharge port 9) is connected above the pipette 5. When sucking a sample into the sample suction tip (pipette tip 4), air is sucked into the pipette 5 from the air tube (air discharge port 9). When taking out the sample in the sample suction tip (pipette tip 4), air is supplied from the air tube (air suction port 8) into the pipette 5. By controlling the intake of air and the supply of air from the air tube (air discharge port 9 and air suction port 8), the suction and extraction speeds of the sample into the sample suction tip (pipette tip 4) can be controlled.

[0003] Also, when sucking the evaporation prevention liquid 3 and the supernatant of the sample 1 into the sample suction tip (pipette tip 4), the height is adjusted by the expansion and contraction of the chip lifting and lowering unit (telescopic stand 11). After positioning the tip of the sample suction tip (pipette tip 4) below the interface between the evaporation prevention liquid 3 and the sample 1 in the sample tube (centrifuge tube 2) where the centrifugation process is completed, suction from the air tube (air suction port 9) is started to suck the evaporation prevention liquid 3 and the supernatant of the sample 1 into the sample suction tip (pipette tip 4). Furthermore, when the liquid level in the sample tube (centrifuge tube 2) drops due to the suction of the evaporation prevention liquid 3 and the sample 1, and the sample suction tip (pipette tip 4) detaches from the liquid surface, the system detects the change in negative pressure caused by the start of air being drawn in from the tip of the sample suction tip (pipette tip 4) and automatically stops suction from the air tube (air suction port 9).

[0004] Furthermore, after recovering the evaporation prevention liquid 3, the pellets to be recovered from the centrifuged sample 1 are recovered using a similar aspiration method or syringe. [Prior art documents] [Patent Documents]

[0005] [Patent Document 1] Japanese Patent Publication No. 2009-145260 [Overview of the project] [Problems that the invention aims to solve]

[0006] However, there was still room for improvement in the sample aspiration tips of dispensing devices known from the above-mentioned patent documents, etc.

[0007] In other words, in the sample suction tip known from Patent Document 1, the tip for aspirating the evaporation prevention liquid and the sample is located on the central axis of the sample suction tip. Therefore, when collecting the sample with the sample suction tip and dispensing it into an empty sample tube, it is not possible to gently inject the sample along the inner wall of the sample tube. There is a risk that the sample may splash and overflow from the sample tube due to the impact when injecting the sample into the sample tube. Furthermore, when injecting the sample into the sample tube, it was easy to draw in ambient air, and if the sample was a substance that oxidizes easily, there was a risk that it would mix with the air and deteriorate. Furthermore, if a specific gravity solution was pre-filled into the sample tube, there was a risk that the force of injecting the sample into the sample tube could cause the specific gravity solution and the sample to mix, blurring the boundary between the liquid surfaces.

[0008] Furthermore, if the amount of sample to be aspirated decreases and the liquid level of the sample cannot be maintained uniformly, causing it to concentrate in one part of the sample tube, there was a risk that the tip of the sample aspirator tip would not be able to reach the sample, making aspiration impossible.

[0009] The present invention aims to solve these problems by providing a sample aspiration tip that has a simple structure, can reliably aspirate even small amounts of sample where a uniform liquid level cannot be maintained, and can quietly inject the aspirated sample into the sample tube without the sample splashing or mixing with other liquids inside the sample tube. [Means for solving the problem]

[0010] The sample aspiration tip of the present invention is a cylindrical sample aspiration tip having an attachment portion with an upper opening that can be connected to a dispensing device for dispensing a sample such as blood in a sample tube via an air tube for supplying and exhausting air, an intermediate portion with a holding portion for temporarily holding the aspirated sample, and a tip portion with a discharge port for aspirating / discharging the sample, wherein the attachment portion is formed on the upper part of the intermediate portion, the tip portion is formed on the lower part of the intermediate portion, the attachment portion, the intermediate portion and the tip portion communicate with the upper opening, the holding portion and the discharge port, part or all of the intermediate portion extending from the tip portion forms an insertion portion, the maximum outer diameter of the insertion portion is formed to be smaller than at least the inner diameter of the opening of the sample tube, the center of the discharge port is located away from the central axis of the intermediate portion, and the discharge port is opened on a plane perpendicular to the central axis of the intermediate portion. The tip portion has a tip connecting base connected to the intermediate portion and a discharge cylinder connecting the lower end of the tip connecting base to the discharge port, the central axis of the tip connecting base is the same as the central axis of the intermediate portion, and the discharge cylinder connects the tip connecting base and the discharge port at the shortest distance. This solves the aforementioned problem. [Effects of the Invention]

[0011] According to the sample aspiration tip of claim 1, since the center of the discharge port is located away from the central axis of the intermediate part, even with a small amount of sample where a uniform liquid level can no longer be maintained in the sample tube, for example, the discharge port can be easily brought closer to the sample simply by rotating the sample aspiration tip so that the central axis of the intermediate part becomes the center of rotation, without tilting the sample tube or the sample aspiration tip. Furthermore, the discharge port can be easily brought close to the inner wall of the sample tube, allowing the aspirated sample to be gently injected along the inner wall of the sample tube. This prevents the sample from splashing or mixing with other liquids or air within the sample tube, and also helps maintain a clear boundary between the sample and other liquids within the sample tube. Furthermore, since the discharge port opens on a plane perpendicular to the central axis of the middle section, the discharge port opens in a direction opposite to the liquid surface, ensuring that only the sample is reliably aspirated, even if the sample being aspirated is a thin separation layer within the sample tube. Furthermore, the central axis of the tip connection base is the same as the central axis of the intermediate section, and the discharge cylinder connects the tip connection base and the discharge port at the shortest distance. Therefore, when injecting a sample into the sample tube, the sample flows through the inclined discharge cylinder from the tip connection base to the discharge port, flowing smoothly along the inner wall surface of the sample tube, allowing for even quieter sample injection.

[0012] According to the configuration described in claim 2, the distance from the central axis of the intermediate section to the outermost part of the lower end of the discharge port is greater than or equal to the distance from the central axis of the intermediate section to the outermost part of the insertion section. Therefore, the discharge port can be brought closer to the inner wall surface of the sample tube without tilting the sample tube or the sample aspiration tip, and the aspirated sample can be injected more reliably and quietly along the inner wall surface of the sample tube.

[0013] Claim 3 According to the configuration described, the central axis of the tip connection base is the same as the central axis of the intermediate section, and the central axis of the discharge cylinder is tilted 20 to 80 degrees from the central axis of the tip connection base. Therefore, when injecting a sample into the sample tube, the sample flows through the discharge cylinder, which is tilted 20 to 80 degrees from the tip connection base to the discharge port, and can flow smoothly along the inner wall surface of the sample tube, allowing for even quieter sample injection.

[0014] Claim 4 With the sample aspiration tip described herein, the center of the discharge port is located away from the central axis of the intermediate section. Therefore, even with a small amount of sample where a uniform liquid level can no longer be maintained in the sample tube, the discharge port can be easily brought closer to the sample simply by rotating the sample aspiration tip so that the central axis of the intermediate section becomes the center of rotation, without tilting the sample tube or the sample aspiration tip. Furthermore, the discharge port can be easily brought close to the inner wall of the sample tube, allowing the aspirated sample to be gently injected along the inner wall of the sample tube. This can prevent the sample from splashing or mixing with other liquids, air, etc. inside the sample tube, and can also clearly maintain the boundary with other liquids inside the sample tube. In addition, among the surfaces of the intermediate part, on the side in the direction where the outermost part of the discharge port is arranged, there are formed indicating protrusions protruding radially outward. Therefore, even without directly checking the inside of the sample tube from the outside, the position of the discharge port can be grasped only by checking the direction of the indicating protrusions. This enables accurate grasping of the position of the discharge port, reliable aspiration of the sample to be aspirated, and injection of the sample along the inner wall surface of the sample tube to the discharge port, even if, for example, the sample tube or the sample is opaque or in an operation in a dark room.

Brief Description of the Drawings

[0015] [Figure 1] Schematic diagram of a dispensing device P equipped with a sample aspiration chip 100 according to an embodiment of the present invention. [Figure 2] Front view of a sample aspiration chip 100 according to an embodiment of the present invention. [Figure 3] Enlarged view of part A of a sample aspiration chip 100 according to an embodiment of the present invention. [Figure 4] Front view showing the aspiration procedure 1 of whole blood BW in a sample tube S1 by a dispensing device P equipped with a sample aspiration chip 100 according to an embodiment of the present invention. [Figure 5] Front view showing the aspiration procedure 2 of whole blood BW in a sample tube S1 by a dispensing device P equipped with a sample aspiration chip 100 according to an embodiment of the present invention. [Figure 6] Front view showing the aspiration procedure 3 of whole blood BW in a sample tube S1 by a dispensing device P equipped with a sample aspiration chip 100 according to an embodiment of the present invention. [Figure 7] Front view showing the injection procedure 1 of whole blood BW into a sample tube S2 by a dispensing device P equipped with a sample aspiration chip 100 according to an embodiment of the present invention. [Figure 8] Front view showing the injection procedure 2 of whole blood BW into a sample tube S2 by a dispensing device P equipped with a sample aspiration chip 100 according to an embodiment of the present invention. [Figure 9] Front view showing the suction procedure 1 of mononuclear cells MS in the sample tube S3 by the dispensing device P equipped with the sample suction chip 100 according to an embodiment of the present invention. [Figure 10] Front view showing the suction procedure 2 of mononuclear cells MS into the sample tube S2 by the dispensing device P equipped with the sample suction chip 100 according to an embodiment of the present invention.

Mode for Carrying Out the Invention

[0016] Hereinafter, the sample suction chip 100 according to an embodiment of the present invention will be described based on the drawings. Note that the dispensing device P is not shown except for some components.

[0017] As shown in FIG. 1, the dispensing device W is a device that sucks a sample such as blood contained in a sample tube and dispenses it into another sample tube, or sucks a desired component from the sample tube, and has a piston lifting unit PM, a pump P, and a chip lifting unit TM. The piston lifting unit PM is connected to a piston rod PL having a piston PT at its tip so as to be able to move up and down. The pump P can perform operations of sucking and dispensing a sample by the sample suction chip 100 described later by the up and down movement of the piston PT, and is connected to a holder H via an air tube T. A differential pressure differential pressure sensor PS is attached to the air tube T connecting the pump P and the holder H. The holder H is connected to the chip lifting unit TM so as to be able to move up and down and rotate. Further, the dispensing device W further has a control unit (not shown) that can automatically perform suction work and dispensing work by controlling the piston lifting unit PM and the chip lifting unit TM.

[0018] As shown in Figures 2 and 3, a sample suction tip 100 according to one embodiment of the present invention is formed in a hollow cylindrical shape and is detachably attached to a holder H. It has a mounting portion 110 attached to the holder H, an intermediate portion 120 connected to the lower part of the mounting portion 110, and a tip portion 130 connected to the lower part of the intermediate portion 120.

[0019] The mounting portion 110 has an upper opening 111 and is connected to the air tube T via the holder H in a detachable manner.

[0020] The intermediate section 120 has a holding section 121 for temporarily holding the aspirated sample and an insertion section 122 that enters the sample tube, and the outer surface of the insertion section 122 is formed in a tapered shape, with the outer diameter gradually decreasing as it goes downwards. Furthermore, an indicator projection 123 is formed on the upper part of the intermediate section 120, projecting radially outward.

[0021] The tip portion 130 has a tip connection base 131 that connects to the lower end of the intermediate portion 120, a discharge pipe 132 that extends downward from the tip connection base 131, and a discharge port 133 that opens from the lower end of the discharge pipe 132. The discharge cylinder 132 is formed to extend downward from the tip connection base 131 and to be inclined in the direction of the protruding indicator projection 123, and the central axis C of the discharge cylinder 132 is formed to be inclined from the central axis B of the intermediate portion 120. The discharge port 133 is opened in a plane perpendicular to the central axis B of the intermediate section 120.

[0022] Next, the procedure for aspirating a sample (whole blood BW) using a dispensing device W equipped with a sample aspiration tip 100 according to one embodiment of the present invention will be explained with reference to Figures 4 to 6. For illustrative purposes, Figures 4 through 6 do not show the piston lifting unit PM, piston rod PL, piston PT, pump P, or differential pressure sensor PS.

[0023] First, as shown in Figure 4, a sample tube S1 containing centrifuged blood (plasma BP and whole blood BW) is fixed below the sample aspiration tip 100. The control unit (not shown) instructs the tip lifting unit TM to lower the sample aspiration tip 100 together with the holder H, and as shown in Figure 5, the tip portion 130 enters the sample tube S1. At this time, the pump P is operating to draw in a small amount of air, and air is being drawn into the sample suction tip 100 from the discharge port 133.

[0024] In this state, when the outlet 133 reaches the plasma BP in the sample tube S1, a small amount of plasma BP is aspirated from the outlet 133, which changes the resistance of the aspiration from the outlet 133, and consequently changes the output value of the differential pressure sensor PS. Furthermore, when the sample aspiration tip 100 is lowered and the discharge port 133 reaches the whole blood BW layer, the output value of the differential pressure sensor PS changes again because the resistance to aspiration of plasma BP and whole blood BW is different. The control unit (not shown) reads the change in the output value of the differential pressure sensor PS, allowing it to recognize which layer in the sample tube S1 the discharge port 133 has reached. In other words, the control unit (not shown) recognizes that the discharge port 133 has reached the whole blood BW layer, which is the target of aspiration, based on the output value of the differential pressure sensor PS, and issues an instruction to the tip lifting unit TM to stop the descent of the sample aspiration tip 100 at a height that allows sufficient aspiration of the whole blood BW layer.

[0025] Furthermore, as shown in Figure 6, after operating the pump P to aspirate a predetermined amount of whole blood BW from the discharge port 133, the pump P is stopped and the sample aspirator tip 100 is raised by the tip lifting unit TM to detach it from the sample tube S1.

[0026] Next, the procedure for injecting a sample (whole blood BW) using a dispensing device W equipped with a sample aspiration tip 100 according to one embodiment of the present invention will be explained with reference to Figures 7 and 8. For illustrative purposes, Figures 7 and 8 do not show the piston lifting unit PM, piston rod PL, piston PT, pump P, or differential pressure sensor PS.

[0027] First, as shown in Figure 7, the sample tube S2 containing the specific gravity liquid DL is fixed below the sample suction tip 100, and the control unit (not shown) issues an instruction to the tip lifting unit TM to lower the sample suction tip 100 together with the holder H, so that, as shown in Figure 8, the discharge port 133 enters a predetermined position above the specific gravity liquid DL in the sample tube S2.

[0028] Next, the pump P is activated to inject whole blood BW into the sample tube S2 from the discharge port 133. When injecting whole blood BW, it is preferable to inject it gently so as not to mix it with the specific gravity liquid DL. Specifically, it is preferable to bring the discharge port 133 close to the inner circumferential wall of the sample tube S2 and inject the whole blood BW so that it flows gently along the inner circumferential wall of the sample tube S2. Furthermore, in the sample suction tip 100 according to one embodiment of the present invention, the discharge cylinder 132 is formed to extend downward from the tip connection base 131 and to be inclined in the direction of the protruding indicator projection 123. Therefore, without tilting the sample tube S2, the discharge port 133 can be easily brought closer to the inner circumferential wall of the sample tube S2 simply by moving the sample tube S2 or the sample suction tip 100 a small amount horizontally.

[0029] Furthermore, since the central axis C of the discharge cylinder 132 is formed at an angle from the central axis B of the intermediate section 120, when whole blood BW is injected into the sample tube, the whole blood BW can flow smoothly along the inner wall surface of the sample tube S2, allowing for even quieter injection of whole blood BW. Furthermore, the inclination angle D from the central axis B of the central axis C is preferably between 20 and 80 degrees. When the injection of whole blood BW into the sample aspiration tip 100 is complete, the resistance during discharge from the discharge port 133 changes, causing a change in the output value of the differential pressure sensor PS. The control unit (not shown) reads the change in the output value of the differential pressure sensor PS, stops the pump P, and instructs the tip lifting unit TM to raise the sample aspiration tip 100 and detach it from the sample tube S2.

[0030] Next, the procedure for aspirating mononuclear cell masses in a sample tube S3 using a dispensing device W equipped with a sample aspiration tip 100 according to one embodiment of the present invention will be described with reference to Figures 9 and 10. For illustrative purposes, Figures 9 and 10 do not show the piston lifting unit PM, piston rod PL, piston PT, pump P, or differential pressure sensor PS.

[0031] As shown in Figure 9, the sample tube S3 contains separated components of whole blood (BW) and specific gravity solution (DL), with each component forming its own layer. From the bottom of the sample tube S3, the layers are: concentrated red blood cell (BD), specific gravity solution (DL), mononuclear cell mass (MS), and plasma (BP).

[0032] First, the sample tube S3 is fixed below the sample aspiration tip 100, and the control unit (not shown) issues an instruction to the tip lifting unit TM to lower the sample aspiration tip 100 together with the holder H, allowing the discharge port 133 to enter the layer of mononuclear cell MS inside the sample tube S3.

[0033] Next, the mononuclear cell mass layer is aspirated from the outlet 133. However, since the amount of mononuclear cell mass is small, it is necessary to collect as much mononuclear cell mass as possible from the sample tube S3 in order to aspirate a sufficient amount. Furthermore, since the discharge port 133 is opened on a plane perpendicular to the central axis B of the intermediate section 120, the discharge port 133 opens in a direction facing each liquid layer surface, and even if the mononuclear cell MS layer is a thin separation layer in the sample tube S3, only the mononuclear cell MS layer can be reliably aspirated. However, if the layer of mononuclear cell MS continues to be aspirated, the remaining mononuclear cell MS may not be able to maintain their layer within sample tube S3 and may accumulate in a droplet-like manner near the inner wall of sample tube S3. At this time, mononuclear cell MS may accumulate at a position away from the discharge port 133. However, in the sample aspiration tip 100 according to one embodiment of the present invention, the discharge cylinder 132 is formed to extend downward from the tip connection base 131 and is inclined in the direction of the protrusion of the indicator projection 123. Therefore, as shown in Figure 10, by rotating the sample aspiration tip 100 together with the holder H using the tip lifting unit TM, the discharge port 133 can be moved to the position where the mononuclear cell MS have accumulated, and the mononuclear cell MS can be sufficiently aspirated.

[0034] In addition, if the liquid inside the sample tube is opaque, or if a label or the like is wrapped around the outer surface of the sample tube, the height position of the discharge port 133 inside the sample tube may not be visually confirmed. However, as mentioned above, the change in the output value of the differential pressure sensor PS makes it easy to determine which liquid layer the discharge port 133 has reached. Furthermore, for similar reasons, the horizontal position of the discharge port 133 inside the sample tube may not be visually confirmed. However, an indicator projection 123 is formed protruding from the outer circumferential surface of the intermediate portion 120 of the sample suction tip 100, and the discharge cylinder 132 is tilted in the direction of the protrusion of the indicator projection 123. Therefore, by visually confirming the orientation of the indicator projection 123 or using a sensor, the horizontal position of the discharge port 133 inside the sample tube can be easily determined.

[0035] Furthermore, the sample aspirated and injected with the sample aspiration tip 100 is not limited to whole blood; for example, it can be used with blood, body fluids, and various other liquids handled in life science fields such as medicine and physical chemistry. Furthermore, in this embodiment, the sample aspiration tip 100 was attached to the holder H of the dispensing device W to automatically perform the aspiration and injection of the sample. However, the aspiration and injection operations do not have to be automated. For example, the sample aspiration tip 100 may be attached to the tip of a pipette or the like, and the aspiration and injection of the sample may be performed manually.

[0036] Although embodiments of the present invention have been described in detail above, the present invention is not limited to the above embodiments, and various design modifications can be made without departing from the present invention as described in the claims.

[0037] In the embodiments described above, the sample suction tip connected to the holder is described as moving up and down and rotating by a tip lifting unit. However, the method of moving up and down and rotating the sample suction tip is not limited to this. For example, the sample suction tip may be grasped by a robot arm and moved up and down and rotated. Furthermore, although the above-described embodiment was explained assuming that an indicator projection is provided in the intermediate section, the configuration of the intermediate section is not limited to this. For example, the indicator projection may not be present, and a visually identifiable mark may be placed on the outer surface of the intermediate section in the direction in which the discharge cylinder is tilted.

[0038] Furthermore, in the embodiments described above, the outer circumferential surface of the insertion portion was described as being formed in a tapered shape with the outer diameter gradually decreasing as it goes downwards. However, the configuration of the sample aspiration tip is not limited to this. For example, part or all of the insertion portion may be formed in a cylindrical shape with a uniform outer diameter smaller than the inner diameter of the sample tube, or the entire sample aspiration tip may be formed in a tapered shape with the outer diameter gradually decreasing as it goes downwards. Furthermore, although the above-described embodiment was explained assuming that the sample aspiration tip has one discharge port at the lower end of the discharge cylinder, the configuration of the discharge cylinder and discharge port is not limited to this, and for example, two or more discharge cylinders and discharge ports may be provided.

[0039] Furthermore, although the above-described embodiment assumed that the dispensing device has a differential pressure sensor, the configuration of the dispensing device is not limited to this, and for example, it may not have a differential pressure sensor. [Explanation of symbols]

[0040] 100 ··· Sample aspiration tips 110 ··· Mounting part 111...Top opening 120 ··· Middle section 121... Holding part 122 ··· Insertion part 123 ... Indication protrusion 130...Tip 131 ··· Tip connection base 132 ··· Discharge cylinder 133... Discharge port B... Central axis of the middle section C ··· Central axis of the discharge pipe D... The angle of inclination of the central axis of the discharge cylinder as viewed from the central axis of the intermediate section. W... Dispensing device PM ··· Piston Lifting Unit TM ··· Chip lifting unit PL ··· Piston Rod PT... Piston PS... Differential pressure sensor P... Pump T... Air tube H ··· Holder S1, S2, S3... Sample tubes BP...Plasma BW ··· Whole blood DL ··· Density liquid MS...mononuclear cell BD ··· Red blood cell concentrate

Claims

1. A cylindrical sample aspiration tip having an upper opening that can be connected to a dispensing device for dispensing samples such as blood in a sample tube via an air tube, an intermediate section with a holding section for temporarily holding the aspirated sample, and a tip section with a discharge port for aspirating / discharging the sample, The mounting portion is formed on the upper part of the intermediate portion, The aforementioned tip portion is formed at the lower part of the aforementioned intermediate portion, The mounting portion, the intermediate portion, and the tip portion are connected to the upper opening, the holding portion, and the discharge port. A part or all of the intermediate portion extending from the tip portion forms an insertion portion. The maximum outer diameter of the insertion portion is formed to be smaller than at least the inner diameter of the opening of the sample tube. The center of the discharge port is located away from the central axis of the intermediate section. The discharge port is opened in a plane perpendicular to the central axis of the intermediate portion. The tip portion has a tip connecting base connected to the intermediate portion and a discharge cylinder connecting the lower end of the tip connecting base to the discharge port. The central axis of the aforementioned tip connection base is the same as the central axis of the aforementioned intermediate portion. The discharge tube is characterized in that the tip connection base and the discharge port are connected by the shortest possible distance.

2. The sample aspiration tip according to claim 1, characterized in that the distance from the central axis of the intermediate portion to the outermost part of the lower end of the discharge port is greater than or equal to the distance from the central axis of the intermediate portion to the outermost part of the insertion portion.

3. The tip portion has a tip connecting base connected to the intermediate portion and a discharge cylinder connecting the lower end of the tip connecting base to the discharge port. The central axis of the aforementioned tip connection base is the same as the central axis of the aforementioned intermediate portion. The sample aspiration tip according to claim 1, characterized in that the central axis of the discharge cylinder is inclined at an angle of 20 to 80 degrees from the central axis of the tip connection base.

4. A cylindrical sample aspiration tip having an upper opening that can be connected to a dispensing device for dispensing samples such as blood in a sample tube via an air tube, an intermediate section with a holding section for temporarily holding the aspirated sample, and a tip section with a discharge port for aspirating / discharging the sample, The mounting portion is formed on the upper part of the intermediate portion, The aforementioned tip portion is formed at the lower part of the aforementioned intermediate portion, The mounting portion, the intermediate portion, and the tip portion are connected to the upper opening, the holding portion, and the discharge port. A part or all of the intermediate portion extending from the tip portion forms an insertion portion. The maximum outer diameter of the insertion portion is formed to be smaller than at least the inner diameter of the opening of the sample tube. The center of the discharge port is located away from the central axis of the intermediate section. A sample aspiration tip characterized in that, on the surface of the intermediate portion, an indicator projection projecting radially outward is formed on the side in which the outermost part of the discharge port is located.

Images