Mosquito attractant, mosquito bait, and method for attracting mosquitoes

Abstract

To provide a mosquito attractant that can satisfactorily attract mosquitoes.SOLUTION: A mosquito attractant contains at least one selected from the group consisting of seafood-derived components, seaweed-derived components and compounds contained therein.SELECTED DRAWING: None

Description

Technical Field

[0001] The present invention relates to a mosquito attractant, a poisonous bait for mosquitoes, and a method for attracting mosquitoes. More specifically, the present invention relates to a mosquito attractant used for attracting, capturing, or killing mosquitoes, a poisonous bait for mosquitoes using the mosquito attractant, and a method for attracting mosquitoes.

Background Art

[0002] When bitten by mosquitoes, it causes itching and may cause rashes and dermatitis at the bitten area. In particular, some mosquitoes are considered to be hygienically harmful insects because they transmit pathogens such as dengue fever, Zika fever, yellow fever, encephalitis, and malaria.

[0003] Mosquitoes possess an excellent chemical receptor system, including a thermosensor for sensing the body temperature of animals, a taste receptor for sensing taste, an olfactory receptor for sensing volatile substances such as body odor, and a carbon dioxide receptor for sensing carbon dioxide, which is a highly volatile substance, and exhibit various behaviors. For example, female mosquitoes seek blood before laying eggs, approach animals by following the carbon dioxide and body odor exhaled by animals during breathing, detect the body temperature with a thermosensor to find the target animal, and are known to suck blood. In addition, male mosquitoes that do not exhibit blood-sucking behavior and female mosquitoes outside the egg-laying period use flower nectar, fruit juice, sap, etc. as food to obtain energy by ingesting sugars.

[0004] Conventionally, mosquitoes have been captured and killed using traps, and various methods for attracting mosquitoes to the traps have been studied. For example, a mosquito catcher has been proposed that generates carbon dioxide by a chemical reaction and captures mosquitoes by utilizing the habit of mosquitoes gathering around carbon dioxide (Patent Document 1). In addition, a flying pest attractant (Patent Document 2) containing an attractant substance containing vinegar and a fragrance as an active ingredient may be used, or a mosquito attractant (Patent Document 3) containing a mixture of lactic acid and methyl lactate as an active ingredient may be used, or a mosquito attractant (Patent Document 4) containing one or more of lysine, isovaleric acid, alanine, androstenol, and sebum may be used to attract mosquitoes.

Prior Art Documents

[0005] [Patent Document 1] Japanese Patent Publication No. 2000-95623 [Patent Document 2] Japanese Patent Publication No. 2013-151470 [Patent Document 3] Japanese Patent Application Publication No. 6-65005 [Patent Document 4] Japanese Patent Publication No. 2002-241204 [Overview of the Initiative] [Problems that the invention aims to solve]

[0006] However, conventionally proposed attractants do not demonstrate sufficient attractiveness, and there is a need to develop attractants that can more effectively attract mosquitoes. Therefore, the present invention aims to provide a mosquito attractant that exhibits high attractiveness to mosquitoes. [Means for solving the problem]

[0007] As a result of diligent research, the inventors discovered that components derived from fish, shellfish, and seaweed, and the compounds contained therein, have excellent mosquito-attracting properties, leading to the completion of the present invention.

[0008] In other words, the present invention is characterized by the following (1) to (5). (1) A mosquito attractant characterized by containing at least one selected from the group consisting of components derived from fish and shellfish, components derived from seaweed, and compounds contained therein. (2) The mosquito attractant according to (1), characterized in that the fish and shellfish include organisms classified into at least one class selected from the group consisting of Actinopterygii, Malacostraca, Bivalvia and Cephalopoda. (3) The mosquito attractant according to (1) or (2) above, characterized in that the seaweed includes seaweed classified as brown algae. (4) A mosquito bait characterized by containing any one of the mosquito attractants described in (1) to (3) above. (5) A method for attracting mosquitoes, characterized by attracting mosquitoes using a mosquito attractant described in any one of (1) to (3) above. [Effects of the Invention]

[0009] The mosquito attractant of the present invention can strongly attract mosquitoes, thus enabling efficient capture or killing of mosquitoes. [Brief explanation of the drawing]

[0010] [Figure 1] The graphs in Test Example 1 show the mosquito-attracting effect of each food product tested. (a) shows the cumulative total number of mosquitoes that flew in, and (b) shows the cumulative total number of mosquitoes that took the mosquito probes. [Figure 2] This is a perspective view showing the configuration of the olfactometer used in each test. [Figure 3] The figure shows the results of Test Example 2, where (a) is a graph showing the number of insects entering the attractant part of the olfactometer, and (b) is a graph showing the ratio of the number of insects entering the test sample to the control sample. [Figure 4] The figures show the results of Test Example 3; (a) is a graph comparing sample 6 and sample 7, and (b) is a graph comparing sample 6 and sample 8. [Figure 5] The figures show the results of Test Example 4; (a) is a graph showing the results of comparing Sample 9 with the control sample, and (b) is a graph showing the results of comparing Sample 10 with the control sample. [Figure 6] The figures show the results of Test Example 5; (a) is a graph showing the results of comparing Sample 11 with the control sample, and (b) is a graph showing the results of comparing Sample 12 with the control sample. [Figure 7] The figures show the results of Test Example 6, where (a) is a graph comparing sample 13 with the control sample, and (b) is a graph comparing sample 14 with the control sample. [Figure 8]A diagram showing the results of Test Example 7, where (a) is a graph showing the change in the number of invading Aedes albopictus, (b) is a graph showing the change in the number of invading Culex quinquefasciatus, (c) is a graph showing the change in the number of invading Aedes aegypti, and (d) is a graph showing the change in the number of invading Culex pipiens pallens. [Figure 9] A diagram showing the results of Test Example 8, which is a graph showing the number of invading Aedes albopictus and the invasion rate for Specimens 15 to 33. [Figure 10] A diagram showing the results of Test Example 9, which is a graph showing the number of invading Aedes albopictus and the invasion rate for Specimens 12, 16, 21, 29, and 34.

Modes for Carrying Out the Invention

[0011] Hereinafter, the mosquito attractant of the present invention and its production method will be described in detail.

[0012] The mosquito attractant of the present invention contains at least one selected from the group consisting of components derived from seafood, components derived from seaweeds, and compounds contained therein (hereinafter also referred to as the specific components of the present invention).

[0013] (Components derived from seafood and compounds contained in components derived from seafood) Seafood refers to aquatic animals such as fish and shellfish, and seafood includes, for example, Actinopterygii (Phylum Chordata), Malacostraca (Phylum Arthropoda), Bivalvia (Phylum Mollusca), and Cephalopoda (Phylum Mollusca). The Actinopterygii class includes, for example, the Gadiformes (e.g., Gadidae, Pleuronectidae, etc.), the Perciformes (e.g., Sparidae, Scombridae, Carangidae, Cichlidae, Serranidae, Ephippidae, etc.), the Salmoniformes (e.g., Salmonidae, etc.), the Aulopiformes (e.g., Aulopidae, Argentinidae, etc.), etc. The Malacostraca class includes, for example, the Decapoda (e.g., Pandalidae, Penaeidae, Palinuridae, Cancridae, Majidae, Scyliaridae, Paguridae, Xanthidae, Eriphiidae, Grapsidae, Portunidae, Matutidae, Dorippidae, etc.), the Mysidacea, etc. The Bivalvia class includes, for example, the Mytiloida (e.g., Mytilidae, etc.), the Veneroida (Veneridae, etc.), the Ostreoida (e.g., Ostreidae, etc.), etc. The Cephalopoda class includes, for example, the Teuthida (e.g., Sepiidae, etc.), the Myopsida (e.g., Loliginidae, etc.), the Octopoda (e.g., Octopodidae, etc.), etc. Preferably, the fishery products include organisms classified into at least one class selected from the group consisting of these Actinopterygii class, Malacostraca class, Bivalvia class, and Cephalopoda class.

[0014] The components derived from fishery products are components contained in all parts such as the body, internal organs, fins, and shells of fishery products, contain volatile compounds, and usually exist as a mixture. The components derived from fishery products and the compounds contained in the components can be obtained from fishery products themselves or processed fishery products, and can also be obtained by methods such as solvent extraction and steam distillation of fishery products and their processed products, and are not particularly limited.

[0015] Examples of the solvent used for extraction include organic solvents, such as alcohols having 1 to 4 carbon atoms, such as ethanol, methanol, 1-propanol, 2-propanol, 1-butanol, and 2-butanol; ethers such as diethyl ether and tetrahydrofuran; ketones such as acetone; hexane; benzene; toluene; carbon tetrachloride; dimethyl sulfoxide; phenol; mixtures thereof, etc. Among these, alcohols having 1 to 4 carbon atoms, ethers, ketones, and hexane are preferred, and it is more preferred to be extracted by at least one organic solvent selected from the group consisting of methanol, diethyl ether, acetone, and hexane.

[0016] For example, if the seafood is a crab (a type of decapoda), the crab-derived components are those found in the crab's meat, internal organs, and shell, and they contain volatile compounds and usually exist as a mixture.

[0017] The types of crabs used to obtain crab-derived components and compounds contained in crab-derived components are not particularly limited, and examples include snow crab, round snow crab, king crab, hairy crab, Hanasaki crab, oil crab, swimming crab, Japanese spider crab, Heike crab, stone crab, rock crab, Asahi crab, chestnut crab, Chinese mitten crab, freshwater crab, shore crab, etc.

[0018] And, for example, compounds found in crab-derived components include, Sulfur compounds such as dimethyl disulfide and benzyl methyl sulfide; Amines such as trimethylamine, 2,5-dimethylpyrazine, and trimethylpyrazine; Ketones such as 2-butanone, 1-penten-3-one, 2,3-pentanedione, 3,5-octadiene-2-one, 2,3-octanedione, 2-nonanone, 3-decen-2-one, and 2-undecanone; Aldehydes such as propanal, 3-methylbutanal, 2-ethyl-3-methylbutanal, pentanal, (E)-2-pentenal, hexanal, benzaldehyde, (E)-2-hexenal, heptanal, (E)-4-heptenal, (E,E)-2,4-heptadienal, octanal, (E)-2-octenal, nonanal, (E)-2-nonenal, (E,Z)-2,6-nonadienal, (E,E)-2,4-decadienal, (E)-2-dodecenal, etc. Alcohols such as ethanol, 3-methyl-1-butanol, 1-pentanol, 1-penten-3-ol, (Z)-2-penten-1-ol, 2-methyl-2-penten-1-ol, 1-hexanol, 2-ethyl-1-hexanol, 1-hepten-4-ol, 1-octanol, 1-octen-3-ol, 1,7-octanedien-3-ol, 2,7-octadien-1-ol, phenethyl alcohol, (E)-2-nonen-1-ol, 10-undecine-1-ol, 3-tetradecine-1-ol, etc. Esters such as ethyl acetate and octyl propionate; (E)-2-heptene, 3-methylene-heptene, 3-methyl-1,4-heptadiene, 3,3-dimethyl-1,6-heptadiene, (Z)-3-octen-1-ene, (Z,Z)-3,5-octadiene, 2,7-dimethyl-1,7-octadiene, 4-ethyl-octane, 4-ethyl-3-octane, 5-methyl-nonane, decane, 4-ethyl-decane, 2-butyl-1-decene, undecene Hydrocarbons such as cane, 2,5-dimethyl-undecane, 3,8-dimethyl-undecane, dodecane, (E)-5-dodecene, 1,11-dodecadiene, (E)-2-dodecene-4-ene, dodecine, 2-methyl-dodecane, 2,4-dimethyldodecane, 3,5-dimethyldodecane, tridecane, (E)-5-tridecene, 7-methyl-tridecane, tetradecane, pentadecane, hexadecane, etc. These are some examples. These compounds may be included individually or in combination of two or more in the mosquito attractant.

[0019] (Components derived from seaweed and compounds contained in components derived from seaweed) Seaweed refers to algae that grow in the sea, and seaweed includes, for example, the class Phaeophyceae (Heterokonta). The brown algae class consists of seaweed that are brown in color and includes, for example, the Fucales order (e.g., Sargassaceae), the Laminariales order (e.g., Laminariaceae, Lamiaceae), and the Coriolus order (e.g., Mozukuaceae). The seaweeds should preferably include those classified as brown algae.

[0020] The components derived from seaweed are those contained in seaweed, and include volatile compounds, usually existing as a mixture. These components and the compounds contained within them can be obtained from seaweed itself or processed seaweed products, and can also be obtained by methods such as solvent extraction or steam distillation, without any particular limitations. The same organic solvents as those described above can be used as the solvent for extraction.

[0021] For example, if seaweed belongs to the class Phaeophyceae, examples of Phaeophyceae include hijiki, kelp, wakame, and mozuku. Components derived from Phaeophyceae are contained in these, and they contain volatile compounds and usually exist as mixtures.

[0022] Compounds derived from brown algae include, for example, hexanal, (E)-2-hexenal, 2-pentylfuran, acetic acid, 5-methyl-2-furancarbaldehyde, 2,6-nonadienal, pyrrole-2-carboxaldehyde, hexyl dodecanoate, cubenol, (2E)-nonenal(2), 2-nonenol and tetradecanoic acid, hexanal(3), (2E)-hexenol, (3E)-hexenol, and β-cyclosulfate. Examples include Toral (8), β-Homocyclocitral, Dihydroactinidiolide (10), Dictiopterene A (11), B (12), C' (13), D' (14), Dictioprene (15), β-Ionone (16), (7Z,10Z)-Hexadecadienal, (8Z,11Z)-Heptadecadienal (17), (8Z,11Z,14Z)-Heptadecatrienal (18), and (8Z)-Heptadecenal (19). These compounds may be included individually or in combination of two or more in the mosquito attractant.

[0023] Among these, the compounds contained in the components derived from brown algae are preferably compounds contained in hijiki, for example, hexanal, (E)-2-hexenal, 2-pentylfuran, acetic acid, 5-methyl-2-francarbaldehyde, 2,6-nonadienal, pyrrole-2-carboxaldehyde, hexyl dodecanoate, cubenol, (2E)-nonenal(2), 2-nonenol and tetradecanoic acid, hexanal(3), (2E)-hexenol, (3E Examples include (7Z,10Z)-hexenol, β-cyclocitral (8), β-homocitral, dihydroactinidiolide (10), dictopterene A (11), B (12), C' (13), D' (14), dictioprolene (15), β-ionone (16), (7Z,10Z)-hexadecadienal, (8Z,11Z)-heptadecadienal (17), (8Z,11Z,14Z)-heptadecadienal (18), and (8Z)-heptadecenal (19).

[0024] The specific components of the present invention, namely components derived from fish and shellfish, components derived from seaweed, and compounds contained therein, may be included individually in the mosquito attractant, or two or more may be included.

[0025] The specific components of the present invention can be identified by separating and quantifying volatile components collected by leaving a sample in a sealed container for a predetermined time, or components extracted from the sample using a solvent, using a gas chromatograph-mass spectrometer (GC / MS) or a liquid chromatograph-mass spectrometer (LC / MS), or by analysis using a nuclear magnetic resonance (NMR) spectrometer.

[0026] Although the details are not clear, when a mosquito attractant contains the specific components of the present invention, mosquitoes become more strongly attracted to them, and the attractant effect is enhanced.

[0027] In the mosquito attractant, the specific component of the present invention only needs to be present in an effective amount that can attract mosquitoes, and its content is not particularly limited. The mosquito attractant may consist solely of the specific component of the present invention (100% by mass), or it may be contained in a solvent.

[0028] The mosquito attractant of the present invention can be obtained, for example, by using a food product containing the specific component of the present invention, or by mixing the food product with other attractant compounds.

[0029] The mosquito attractant of the present invention may contain additives such as other known attractants, insecticides, antibacterial agents, colorants, ultraviolet absorbers, retainers, lubricants, and solvents, to the extent that they do not inhibit the mosquito-attracting effect.

[0030] Other attractants include, for example, alcoholic beverages such as brandy, whiskey, rum, vodka, shochu, and sake; brewed vinegars such as black vinegar, red vinegar, regular vinegar, apple cider vinegar, and rice vinegar; sugars such as honey, liquid sugar, and maple syrup; lactic acid products such as lactic acid beverages, yogurt, and cheese; fruit juices; and fruit-flavored fragrances.

[0031] Examples of insecticidal ingredients include dichlorvos, fenitrothion, IBTA, IBTE, transfluthrin, metofluthrin, profluthrin, empenthrin, propoxur, phenobucarb, amidoflumeth, dinotefuran, fipronil, hydramethylnon, carbaryl, and boric acid.

[0032] Examples of antibacterial components include chlorine dioxide, iodine, thymol, isopropylmethylphenol, formaldehyde, glutaraldehyde, ethanol, propyl alcohol, phenol, cresol, phenoxyethanol, cetylpyridinium chloride, and parabens.

[0033] Examples of coloring agents include Blue No. 1 and Yellow No. 4.

[0034] Examples of UV absorbers include trithresorcinol triazine compounds.

[0035] Examples of solvents include lower alcohols such as methanol, ethanol, and propanol; hydrocarbon solvents such as liquid paraffin; and water such as distilled water, tap water, and deionized water.

[0036] The dosage form of the mosquito attractant of the present invention can be selected according to the intended use, and may include liquid, paste, solid (jelly, powder, granules, etc.), or impregnated in a material such as volatile paper or porous material. Specifically, a solid mosquito attractant can be obtained by using a liquid mosquito attractant as is, mixing a liquid mosquito attractant with a gelling agent to create a paste or solid, impregnating a water-absorbing polymer with a liquid mosquito attractant, or supporting a liquid mosquito attractant on an inorganic carrier. Furthermore, seafood or seaweed may be used in any state, such as raw, boiled, dried, or grilled.

[0037] (Method of using (attracting) mosquito attractants) The mosquito attractant of the present invention can be filled into a container or impregnated into a fibrous carrier and installed together with a trap. Mosquitoes attracted by the mosquito attractant of the present invention are captured in the trap.

[0038] Conventional and known traps can be used, including those equipped with adhesive sheets and traps that use suction fans to draw in and capture mosquitoes with the force of the wind.

[0039] (Mosquito bait) The mosquito attractant of the present invention can be used together with a mosquito insecticide to form a mosquito bait. That is, the mosquito bait of the present invention contains the above-mentioned mosquito attractant, a mosquito insecticide, and a mosquito bait.

[0040] In mosquito bait, the mosquito attractant of the present invention only needs to be present in an effective amount that can attract mosquitoes, and the amount is not particularly limited and can be adjusted as appropriate.

[0041] The insecticidal components listed above are suitable for use against mosquitoes. In particular, dinotefuran, fipronil, and boric acid are preferred because they do not inhibit the attractiveness of mosquito attractants.

[0042] The form of mosquito bait is not particularly limited, but examples include liquid, paste, and solid (gel, granular, powder).

[0043] Examples of mosquito species that can be attracted in this invention include Aedes albopictus and Aedes aegypti, Culex pipiens, Culex aeruginosa, Culex pipiens, Culex tritaeniorhynchus, and Culex pipiens, and Anopheles mosquitoes, but the invention is not limited to these examples. [Examples]

[0044] The present invention will be further described below based on specific test examples, but the present invention is not limited in any way to the examples below. Samples 1-3 and 6-34 are examples, and samples 4-5 are comparative examples.

[0045] <Test Example 1> The mosquito-attracting effect was confirmed for the following samples 1-4. • Sample 1: Shredded crab meat (Product name: "Red Snow Crab Shredded Meat" manufactured by Nippon Suisan Kaisha, Ltd.) • Sample 2: Rehydrated hijiki seaweed (Yamanaka Foods Co., Ltd.'s "Easy and Convenient Hijiki" (product name) was soaked in water at approximately 70°C for 10 minutes, then soaked in room temperature water for 10 minutes, and drained). • Sample 3: Tuna (sashimi-grade tuna fillets) • Sample 4: Beef tendon (domestic beef tendon)

[0046] (Test method) 1. Approximately 1,200 adult Aedes albopictus mosquitoes (7-10 days old, mixed sexes 1:1) were used as test subjects. 2. The test insects were placed in a mesh cage measuring 25cm (length) x 25cm (width) x 25cm (height) and left in a test chamber at a temperature of 27°C ± 2°C and a humidity of 60% ± 20% for one hour to allow the insects to acclimate. 3. Approximately 10g of the sample was placed on a φ7cm filter paper placed on a φ9cm glass petri dish, and then left to stand in a mesh cage. To prevent the test insects from reacting only to the moisture in the food, a KP cup containing 100mL of tap water was placed inside the mesh cage beforehand. 4. After placing the glass petri dish containing the sample inside the mesh cage, the number of test insects that landed on the sample was counted every 30 seconds for 3 minutes, and the cumulative total number of insects that flew onto the sample was calculated after 3 minutes. Similarly, the number of times the mosquito performed its probing behavior, one of its blood-feeding behaviors, was counted every 30 seconds, and the cumulative total number of mosquitoes performing probing behavior was observed after 3 minutes. The cumulative count is calculated by sequentially adding up the total number of occurrences every 30 seconds. 5. The experiment was conducted three times, and the data was expressed as the average of the cumulative total number of flying insects and the total number of insects engaging in probing behavior three minutes after the start of the experiment.

[0047] The results are shown in Figures 1(a) and 1(b).

[0048] As shown in Figures 1(a) and (b), the cumulative number of mosquitoes attracted to samples 1 (shredded crab meat), 2 (hijiki seaweed), and 3 (tuna) was higher than that of sample 4. Furthermore, samples 1 and 2 were found to strongly exhibit probing behavior (the behavior of determining a blood-feeding site and inserting a needle), which is one of the blood-feeding behaviors of mosquitoes, and it is presumed that the mosquitoes mistook these for a blood source.

[0049] <Test Example 2> A comparative test of the attractiveness of samples 1 (shredded crab meat) and 2 (hijiki seaweed) used in Test Example 1, as well as sample 5 below, was conducted using an olfactometer. • Sample 5: Honey ("Pure Honey" (product name) manufactured by Kato Bee Farm Co., Ltd.)

[0050] (Test method) 1. As test insects, adult Aedes albopictus mosquitoes (7-10 days old, approximately 50 females and 50 males) were used, and tests were conducted on both males and females separately. 2. The test was conducted using an olfactometer 10 as shown in Figure 2. The olfactometer 10 comprises a set of transparent sample boxes (first sample box 1A, second sample box 1B), transparent cylindrical attractants 2A and 2B connected to the first sample box 1A and the second sample box 1B respectively, and a transparent mosquito containment box 3 connected to the attractants 2A and 2B. A check valve 4 is provided at the connection between the mosquito containment box 3 and the attractants 2A and 2B to prevent mosquitoes that have entered the attractants 2A and 2B from re-entering the mosquito containment box 3. Nets are provided at the connection between the first sample box 1A and the attractant 2A, and between the second sample box 1B and the attractant 2B, respectively, to prevent mosquitoes that have entered the attractants 2A and 2B from entering the first sample box 1A and the second sample box 1B. A partition door is provided at the connecting section to seal off odors. When the test begins, the partition door is opened, clean air is circulated into the olfactometer 10, and an airflow of 0.3 m / s is generated from the sample boxes 1A and 1B towards the mosquito containment box 3. 3. The Olfactometer 10 was placed in a test chamber with a temperature of 27°C ± 2°C and a humidity of 60% ± 20%. The test insects were released into the mosquito containment box 3 inside the Olfactometer 10 and allowed to acclimate for 1 hour. 4. A test sample containing approximately 50g of the sample was placed in a φ9cm glass petri dish and set up in the first sample box 1A of the olfactometer 10 to serve as the test group. In the other second sample box 1B, a φ9cm glass petri dish containing 50g of 2% sugar water was set up to serve as the control group. 5. The partition door of the olfactometer 10 was opened to allow the test insects to enter the attractant sections 2A and 2B. The number of test insects that had entered each attractant section was counted 10 to 37 hours after the partition door was opened. 6. The experiment was conducted three times, and the number of insects entering the attraction area and the average of the ratio of the number of insects entering the control sample to the test sample (number of insects entering the test sample / number of insects entering the control sample) were calculated.

[0051] The results are shown in Figures 3(a) and (b).

[0052] As shown in Figures 3(a) and (b), sample 1 (shredded crab meat) showed the strongest attraction to mosquitoes, with a penetration rate into the attractant area more than 10 times higher than that of the control group. Furthermore, in samples 1 and 2, the mosquitoes that entered the attractant area actively engaged in probing behavior, suggesting that they may have been attracted by mistaking the shredded crab meat for a blood source. In contrast, mosquitoes did not exhibit probing behavior in sample 5 (honey).

[0053] <Test Example 3> The following samples 6-8 were tested to confirm their attractiveness. Sample 6 is the same as sample 1. • Sample 6: Shredded crab meat (freshly opened) (Nippon Suisan Kaisha, Ltd. "Red Snow Crab Shredded Meat" (product name), 25g) • Sample 7: Shredded crab meat (1 day volatile product) (Sample 6 left standing at 27°C for 24 hours, 16g) • Sample 8: Shredded crab meat (3-day volatile sample) (Sample 6 left standing at 27°C for 3 days, 4g)

[0054] (Test method) 1. Approximately 100 adult Aedes albopictus mosquitoes (7-10 days old, female) were used as test subjects. 2. Approximately 25g of shredded crab meat from sample 6 was placed in a φ9cm glass petri dish and left uncovered in a test room set to 27°C for a specified time to obtain samples 7 and 8. Sample 7 was obtained after 1 day (24 hours) from the start of standing (1-day volatilization), and sample 8 was obtained after 3 days (3-day volatilization). 3. The test was conducted using the olfactometer described in Test Example 2. Sample 7 or sample 8 was placed in the first sample box 1A of the olfactometer 10, and sample 6 was placed in the other second sample box 1B. 4. The test was conducted in the same manner as in Test Example 2. After opening the partition door, the number of test insects entering each attractant was counted over time, and the cumulative total number of insects that entered was determined. 5. The test was conducted three times, and the average number of insects entering the attraction area was calculated.

[0055] The results are shown in Figures 4(a) and 4(b).

[0056] As shown in Figures 4(a) and (b), there was almost no difference in mosquito attraction between sample 6 (immediately after opening) and sample 7 (1 day after volatilization), but it was confirmed that sample 8 (3 days after volatilization) attracted mosquitoes better than sample 6.

[0057] <Test Example 4> The following samples 9-10 were tested to confirm their attractiveness. • Sample 9: Raw crab meat (approximately 20g of crab meat collected from the legs of Ocean Foods Co., Ltd.'s "Raw Red Snow Crab Leg Portion" product). • Sample 10: Boiled crab meat (approximately 20g of crab leg meat collected after boiling the crab legs used in Sample 9 for 3 minutes and letting them return to room temperature).

[0058] (Test method) 1. Approximately 100 adult Aedes albopictus mosquitoes (7-10 days old, female) were used as test subjects. 2. The test was conducted using the olfactometer described in Test Example 2. Sample 9 or sample 10 was placed in the first sample box 1A of the olfactometer 10 (sample group), and a φ9cm glass petri dish containing 20g of 2% sugar water was placed in the other second sample box 1B (control group). 3. The test was conducted in the same manner as in Test Example 2, and the number of test insects entering each attractant was counted over time after opening the partition door, and the cumulative total number of insects that entered was determined. 4. The test was conducted three times, and the average number of insects entering the attraction area was calculated.

[0059] The results are shown in Figures 5(a) and (b).

[0060] The results in Figures 5(a) and (b) show that mosquitoes were attracted to both raw crab meat (sample 9) and boiled crab meat (sample 10), and that sample 10 had a stronger attractive effect on mosquitoes than sample 9. Furthermore, the mosquitoes' probing behavior was also observed.

[0061] <Test Example 5> The following samples 11 and 12 were tested to confirm their attractiveness. Sample 11 is the same as sample 1. • Sample 11: Shredded crab meat (Nippon Suisan Kaisha, Ltd.'s "Red Snow Crab Shredded Meat" (product name), 20g) • Sample 12: Crab miso (Maruha Nichiro Corporation's "Crab Miso with Crab Meat" (product name), 20g)

[0062] The test was conducted in the same manner as in Test Example 4. The results are shown in Figures 6(a) and (b).

[0063] As shown in Figure 6(b), mosquitoes entered the attractant area of ​​sample 12 (crab miso) immediately after the start of the test, indicating that it has a high attractiveness to mosquitoes, similar to sample 11 (shredded crab meat) shown in Figure 6(a). Furthermore, similar to sample 11, mosquitoes were observed actively probing with their proboscises in sample 12.

[0064] <Test Example 6> The following samples, 13-14, were subjected to tests to confirm their attractiveness. Sample 13: Crab miso (Maruyo Foods Co., Ltd. "Kani Miso" (product name), 20g) • Sample 14: Rehydrated hijiki seaweed (20g of "Hijiki" (product name) manufactured by Marumo Co., Ltd., soaked in water at approximately 70°C for 10 minutes, then soaked in room temperature water for 10 minutes, and drained).

[0065] The test was conducted in the same manner as in Test Example 4. The results are shown in Figures 7(a) and (b).

[0066] The results in Figures 7(a) and (b) confirm that both sample 13 (crab miso) and sample 14 (hijiki seaweed) have a high attractiveness to mosquitoes.

[0067] <Test Example 7> A test was conducted to confirm the mosquito-attracting effect of sample 12 (Maruha Nichiro Corporation's "Crab Miso with Crab Meat" (product name), 20g) used in Test Example 5, for each mosquito species. The test insects used were as follows: • Adult Asian tiger mosquitoes: 7-10 days old, female, approximately 100 individuals • Adult Aedes aegypti mosquitoes: 7-10 days old, female, approximately 100 individuals • Adult Culex pipiens: 7-10 days old, female, approximately 100 individuals • Adult Tropic mosquitoes: 7-10 days old, female, approximately 100 individuals

[0068] (Test method) 1. The test was conducted using the olfactometer described in Test Example 2. Sample 12 was placed in the first sample box 1A of the olfactometer 10 (sample group), and a φ9cm glass petri dish containing 20g of 2% sugar water was placed in the other second sample box 1B (control group). 2. The test was conducted in the same manner as in Test Example 2. From 9 to 25 hours after opening the door, the number of test insects entering each attractant was counted over time, and the cumulative total number of insects entering was determined. Since house mosquitoes are nocturnal blood-sucking insects, the test was conducted continuously for at least one night. 3. The test was conducted three times, and the average number of insects entering the attraction area was calculated.

[0069] The results are shown in Figures 8(a) to 8(d).

[0070] The results in Figures 8(a) to 8(d) show that the attractant is effective not only against Aedes albopictus but also against Aedes aegypti, Culex pipiens, and Culex aegypti. In particular, high attractiveness was confirmed against Aedes albopictus and Aedes aegypti, indicating that it exhibits excellent attractiveness against Aedes mosquitoes of the genus Aedes.

[0071] <Test Example 8> The following samples, 15-33, were subjected to tests to confirm their attractiveness. • Sample 15: Pacific white shrimp ("Frozen Pacific white shrimp from India, extra large (farmed)" (processed by Daikokuten Bussan Co., Ltd.)) • Sample 16: Northern shrimp (Hyogo Prefecture-produced raw sweet shrimp (processed by Daikokuten Bussan Co., Ltd.)) • Sample 17: Akiami shrimp (HAMAICHI Co., Ltd.'s "G-Ami Ebi" (product name)) • Sample 18: Krill (Koharu Co., Ltd. "Tsurimonogatari Raw Extreme S Size" (product name)) • Sample 19: Oil flounder ("Aburakarei" (processed by Daikokuten Bussan Co., Ltd.)) • Sample 20: Red sea bream ("Kochi Prefecture Olive-Aged Red Sea Bream Sashimi Strips (Cultivated)" (Processed by Daikokuten Bussan Co., Ltd.)) • Sample 21: Coho salmon ("Chilean Coho Salmon Fillet (Cultivated), Thawed" (Processed by Daikokuten Bussan Co., Ltd.)) • Sample 22: Yellowfin tuna ("Kagoshima Prefecture-produced yellowfin tuna for sashimi (thawed)" (processed by Daikokuten Bussan Co., Ltd.)) • Sample 23: Yellowtail ("Natural Yellowtail Fillet from Nagasaki Prefecture" (processed by Daikokuten Bussan Co., Ltd.)) • Sample 24: Pacific mackerel ("Wakayama Prefecture Pacific Mackerel Fillet" (processed by Daikokuten Bussan Co., Ltd.)) • Sample 25: Japanese horse mackerel ("Small horse mackerel from Kochi Prefecture" processed by Daikokuten Bussan Co., Ltd.) • Sample 26: Ayu ("Gifu Prefecture-produced Ayu (farmed)" (processed by Daikokuten Bussan Co., Ltd.)) • Sample 27: Satsuki trout ("Tokushima Prefecture farmed Amago trout" (processed by Daikokuten Bussan Co., Ltd.)) • Sample 28: Oysters ("Raw Oysters" (obtained from Yamashita Son Shoten Co., Ltd.)) • Sample 29: Clams ("Shelled Clams (for cooking)" (processed by Kamoi Foods Co., Ltd.)) • Sample 30: Scallop ("Scallop adductor muscle for sashimi, Hokkaido-produced" (processed by Hinase Shopping Center Co., Ltd.)) • Sample 31: Japanese flying squid ("Chinese-produced Japanese flying squid (for thawing and cooking)" (processed by Yoshida Shoji Co., Ltd.)) • Sample 32: Japanese spear squid ("Japanese spear squid from Miyagi Prefecture" (processed by AEON Retail Co., Ltd.)) • Sample 33: Common octopus ("Steamed Octopus (True Octopus) for Sashimi, Made with Moroccan Ingredients" (Processed by AEON Retail Co., Ltd.))

[0072] (Test method) 1. Approximately 100 adult Aedes albopictus mosquitoes (7-10 days old, female) were used as test subjects. 2. The test was conducted using the olfactometer described in Test Example 2. 20g of the sample was placed in the first sample box 1A of the olfactometer 10 (sample group), and a φ9cm glass petri dish containing 20g of 2% sugar water was placed in the other second sample box 1B (control group). 3. The test was conducted in the same manner as in Test Example 2, and the number of test insects that had entered each attractant was counted at any time between 8 and 11 hours after the partition door was opened. 4. The experiment was conducted three times, and the average number of individuals entering the attraction area and the average percentage of individuals entering the attraction area on the sample side were calculated. Invasion rate to the attraction area on the sample side (%) = Number of individuals invading the attraction area on the sample side / (Number of individuals invading the attraction area on the sample side + Number of individuals invading the attraction area on the control side) × 100

[0073] The results are shown in Table 1 and Figure 9. In the graph in Figure 9, the first vertical axis on the left represents the number of insects entering the attraction area, and the second vertical axis on the right represents the rate of insect entry into the attraction area on the sample side.

[0074] [Table 1]

[0075] As shown in Table 1 and Figure 9, all samples showed a penetration rate of 75% or more into the attractant area on the sample side, indicating high attractiveness to mosquitoes. In particular, samples 15 (Litopenaeus vannamei shrimp), 16 (Primula japonica), 18 (Krill), 21 (Coho salmon), 22 (Yellowfin tuna), 25 (Japanese horse mackerel), 27 (Satsuki trout), and 29 (Clam) had extremely high penetration rates of 90% or more into the attractant area.

[0076] <Test Example 9> The attractive effect of the extract was confirmed for sample 12 (crab miso) used in Test Example 5, sample 16 (northern shrimp), sample 21 (coho salmon), and sample 29 (clams) used in Test Example 8, as well as sample 34 (crab meat) below. • Sample 12: Crab miso ("Crab Miso with Crab Meat" manufactured by Maruha Nichiro Corporation) (product name) • Sample 16: Northern shrimp (Hyogo Prefecture-produced raw sweet shrimp (processed by Daikokuten Bussan Co., Ltd.)) • Sample 21: Coho salmon ("Chilean Coho Salmon Fillet (Cultivated), Thawed" (Processed by Daikokuten Bussan Co., Ltd.)) • Sample 29: Clams ("Shelled Clams (for cooking)" (processed by Kamoi Foods Co., Ltd.)) • Sample 34: Crab meat (Fukui Canned Foods Co., Ltd.'s "Red Snow Crab in Water (Flakes)" (product name))

[0077] (Preparation of extract) Sample 16 (Northern shrimp), Sample 21 (coho salmon), and Sample 29 (clams) were ground using a food processor to make surimi (fish paste). Approximately 20g each of the surimi from sample 12 (crab miso), sample 16 (northern shrimp), sample 21 (silver salmon), and sample 29 (clam) was weighed onto cylindrical filter paper and placed in a Soxhlet extractor. Sample 34 (crab meat) was weighed out at approximately 25g in a φ9cm glass petri dish, left to stand at 27°C for 3 days, then transferred to cylindrical filter paper and placed in a Soxhlet extractor. Hexane and methanol were refluxed at approximately 80°C, acetone at approximately 70°C, and diethyl ether at approximately 40°C for approximately 5 hours, and solvent-soluble components were extracted from each sample. After refluxing, the solution was reduced using an evaporator and concentrated under 40°C conditions until boiling ceased to obtain the extract.

[0078] (Test method) The test was conducted using the olfactometer described in Test Example 2. The extracts obtained above were diluted with the extraction solvent and the amount of extract used in each test was adjusted so that the amount of compound in the extract used in one test was the same as the amount of compound in 20g of fish and shellfish before extraction. Hexane, diethyl ether, acetone, and methanol extracts of crab miso and crab meat, and hexane and diethyl ether extracts of northern shrimp and coho salmon were diluted with the extraction solvent so that each 3g of the diluted solution contained the compounds present in 20g of the raw seafood before extraction, and then used in the test. 3g of the extraction solvent was placed in the control group. The acetone and methanol extracts of northern shrimp and coho salmon were used in the test without dilution with the extraction solvent, and the treatment volume was adjusted to match the amount of compounds present in 20g of the seafood before extraction. 20g of 2% sugar water was used as the control group.

[0079] Approximately 100 adult Aedes albopictus mosquitoes (7-10 days old, female) were used as test insects. The experiment was conducted in the same manner as in Test Example 8, and the number of test insects that had entered each attractant was counted at any time between 7 and 12 hours after opening the partition door. The experiment was conducted three times, and the average number of individuals entering the attraction area and the average percentage of individuals entering the attraction area on the sample side were calculated.

[0080] The results are shown in Table 2 and Figure 10. In the graph in Figure 10, the first vertical axis on the left represents the number of insects entering the attraction area, and the second vertical axis on the right represents the rate of insect entry into the attraction area on the sample side.

[0081] [Table 2]

[0082] The results in Table 2 and Figure 10 show that the mosquito intrusion rate into the sample groups was equal to or higher than that of the control group for all samples, indicating that the hexane, diethyl ether, acetone, and methanol extracts were all attractive to mosquitoes. This suggests that these extracts contain compounds that attract mosquitoes. [Explanation of symbols]

[0083] 1A First sample box 1B Second specimen box 2A,2B Attraction part 3 Mosquito containment box 4. Check valve 10 Olfactometer

Claims

1. A mosquito attractant characterized by containing processed seafood products as an active ingredient, wherein the seafood products are selected from the group consisting of coho salmon, yellowfin tuna, horse mackerel, cherry salmon, krill, and clams.

2. A mosquito attractant characterized by containing at least one of processed crab products and processed hijiki seaweed products as an active ingredient.

3. A mosquito bait characterized by containing the mosquito attractant described in claim 1 or 2.

4. A method for attracting mosquitoes, characterized by attracting mosquitoes using a mosquito attractant according to claim 1 or 2.

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