Pacific Ocean Antigen Recovery System and Pacific Ocean Antigen Recovery Method

Abstract

This system (A) for recovering an antigen in a solvent comprises charged particles, a mixing unit (4), and a recovery unit (7), wherein: the charged particles each contain 3-70 mol% of a cationic lipid, 1-20 mol% of a PEG-containing lipid, and 20-50 mol% of cholesterol; the surfaces of the charged particles are modified with antibodies; the average zeta potential of the charged particles is -20 to +15 mV; the mixing unit (4) is configured such that the charged particles are added to a solvent (C) and the charged particles and the solvent (C) are stirred in the mixing unit (4); the recovery unit 7 is configured to recover the charged particles mixed with the solvent (C); and the recovery unit (7) is an anionic adsorber that includes an adsorption carrier having a surface on which a compound having an anionic functional group is immobilized.

Claims

[Claim 1] Charged particles and, Mixing section, A solvent antigen recovery system comprising a recovery unit, The charged particles contain 3 to 70 mol% cationic lipids, 1 to 20 mol% PEG-containing lipids, 20 to 50 mol% cholesterol, and 0 to 65 mol% neutral lipids. The surface of the charged particle is modified with an antibody. The average zeta potential of the charged particles is -20 to +15 mV. The mixing unit is configured such that, within the mixing unit, the charged particles are introduced into the solvent and the charged particles and the solvent are stirred. The recovery unit is configured to recover the charged particles mixed with the solvent, The aforementioned recovery unit is a solvent-based antigen recovery system, which is an anionic adsorbent equipped with an adsorption carrier on which a compound having an anionic functional group is immobilized on the surface. [Claim 2] The aforementioned anionic functional group is a sulfate group, a carboxyl group, or a sulfo group. The solvent-based antigen recovery system according to claim 1, wherein the compound is dextran sulfate, polyacrylic acid, or polystyrene sulfonic acid. [Claim 3] The charged particles contain 10 to 60 mol% of the cationic lipid, 6 to 14 mol% of the PEG-containing lipid, 20 to 50 mol% of the cholesterol, and 0 to 30 mol% of the neutral lipid. The solvent-based antigen recovery system according to claim 1, wherein the average zeta potential of the charged particles is -15 to +10 mV. [Claim 4] The charged particles contain 20 to 60 mol% of the cationic lipid, 8 to 14 mol% of the PEG-containing lipid, 20 to 50 mol% of the cholesterol, and 0 to 25 mol% of the neutral lipid. The solvent-based antigen recovery system according to claim 1, wherein the average zeta potential of the charged particles is -10 to +5 mV. [Claim 5] The solvent-based antigen recovery system according to claim 1, wherein the average particle size of the charged particles is 200 nm or less. [Claim 6] The solvent-based antigen recovery system according to claim 1, wherein the average particle size of the charged particles is 100 nm or less. [Claim 7] The solvent-based antigen recovery system according to claim 1, wherein the average zeta potential of the adsorption carrier is -20 mV or less. [Claim 8] The solvent-based antigen recovery system according to claim 1, wherein the average zeta potential of the adsorption carrier is -30 mV or less. [Claim 9] The solvent-based antigen recovery system according to claim 1, wherein the concentration of the charged particles in the mixing section is 0.01 to 2.0 mg / ml. [Claim 10] The mixing section and the recovery section are connected by a first flow path. The mixing section is further connected to a second flow path, The aforementioned recovery unit is further connected to a third flow path, The solvent antigen recovery system includes a pump configured to control the flow rate of the solvent, The solvent-based antigen recovery system according to claim 1, wherein the pump is configured to adjust the flow rate of the solvent in the third channel to 100 ml / min or less.

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