Improved virus detection method

A one-step RT-PCR method using an anionic polymer and polar organic solvent for RNA virus detection simplifies the process, enhances sensitivity, and reduces contamination risks, addressing the inefficiencies of traditional RNA purification methods.

JP7875451B2Active Publication Date: 2026-06-18TOYOBO CO LTD
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Patent Information

Authority / Receiving Office
JP · JP
Patent Type
Patents
Current Assignee / Owner
TOYOBO CO LTD
Filing Date
2021-07-21
Publication Date
2026-06-18

AI Technical Summary

⚠Technical Problem

Existing methods for detecting RNA viruses, particularly coronaviruses like SARS-nCOV-2, require complex RNA purification steps, which are time-consuming and prone to contamination, and introduce PCR inhibitors, leading to reduced sensitivity and increased risk of false positives.

⚗Method used

A one-step RT-PCR method that mixes a sample with an anionic polymer and a polar organic solvent, followed by heat treatment, then adds a one-step RT-PCR reaction solution, eliminating the need for RNA purification and reducing the influence of contaminants.

🎯Benefits of technology

This method enables rapid, sensitive detection of RNA viruses without RNA purification, reducing contamination risks and improving test efficiency, accuracy, and worker safety by minimizing handling of infectious samples.

✦ Generated by Eureka AI based on patent content.

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Abstract

The present invention provides a method and a kit for making it possible to detect the presence / absence of virus RNA by one-step RT-PCR, in which the RNA is not isolated from a specimen, and a sample that has been subjected to only a simple thermal pre-treatment is added to a reaction solution. In particular, provided is a means for detecting, at a high sensitivity, the presence of a coronavirus in a specimen. A method for testing the presence of an RNA virus according to one embodiment of the present invention comprises steps (1) to (4): (1) a step for preparing a liquid mixture that contains a specimen which has not been subjected to RNA purification, an anionic polymer, and a polar organic solvent; (2) a step for heating the liquid mixture; (3) a step for adding, to the liquid mixture which has been heated, a 1-step RT-PCR reaction solution that includes (i) a reverse transcriptase and a DNA polymerase or (ii) a DNA polymerase having reverse transcription activity; and (4) a step for performing 1-step RT-PCR reaction after tightly sealing the reaction container.
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