Method for preparing polynucleotides using a polyvalent cation salt composition

A method using polyvalent cation salts and reverse-phase chromatography enhances nuclease resistance and specificity of polynucleotides, addressing the limitations of existing preparations for anti-sense agents targeting telomerase in cancer cells.

JP7883361B2Inactive Publication Date: 2026-07-01GERON CORP
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Patent Information

Authority / Receiving Office
JP · JP
Patent Type
Patents
Current Assignee / Owner
GERON CORP
Filing Date
2021-07-01
Publication Date
2026-07-01
Estimated Expiration
Not applicable · inactive patent

AI Technical Summary

Technical Problem

Existing methods for preparing polynucleotides, such as polynucleotide N3'→P5' phosphoramidates and polynucleotide N3'→P5' thiophosphorumamides, face challenges in achieving high nuclease resistance and specificity, particularly for applications like anti-sense agents targeting telomerase activity in cancer cells.

Method used

A method involving the use of a polyvalent cation salt to precipitate polynucleotides with N3'→P5' thiophosphoramidate bonds, followed by reverse-phase chromatography to isolate and purify polynucleotides with specific sequences complementary to human telomerase RNA, enhancing nuclease resistance and cell uptake.

Benefits of technology

The method produces polynucleotides with improved nuclease resistance and specificity, suitable for targeting telomerase activity in cancer cells, facilitating the development of therapeutic agents.

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Patent Text Reader

Abstract

To provide methods for the preparation of a polynucleotide with a multivalent cation salt composition.SOLUTION: Aspects of the disclosure include methods for the preparation of a polynucleotide. In some embodiments, the method includes: contacting a first polynucleotide composition including a polynucleotide that has a sequence of 7 or more nucleoside subunits and in which at least two of the nucleoside subunits are joined by a N3'→P5' thiophosphoramidate inter-subunit linkage, and including non-target synthetic products and reagents, with a multivalent cation salt, to precipitate a polynucleotide salt including at least one multivalent cation counterion; and separating the polynucleotide salt from the contacted first polynucleotide composition to produce a second polynucleotide composition including the polynucleotide salt.SELECTED DRAWING: Figure 2
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