Bifidobacterium animalis subsp. lactis strain and use thereof for improving hair or scalp condition

Abstract

The present invention relates to use of a novel Bifidobacterium animalis subsp. lactis DS008 strain for improving hair or scalp condition. The strain and a cosmetic composition comprising same have the effects of strengthening hair roots, improving hair elasticity, improving hair texture, improving hair gloss, improving damaged hair, or increasing hair thickness, and thus can have various applications for the improvement, prevention or treatment of hair or scalp condition.

Description

Bifidobacterium animalis subspecies lactis strain and its use for improving hair or scalp condition

[0001] The present invention relates to a novel microorganism, its lysate, culture solution, extract, and its use for improving hair or scalp condition.

[0002] Hair undergoes a cyclical cycle of growth (anagen), regression (catagen), resting (telogen), and shedding. Hair is composed of 90% keratin, composed of 18 amino acids, and approximately 10% water. Its constituent elements include carbon, oxygen, nitrogen, hydrogen, and sulfur.

[0003] Hair consists of the root, which participates in growth within the skin, and the shaft, which is exposed to the outside of the skin. A cross-section of a hair shaft consists of the cuticle, cortex, and medulla. The cortex accounts for over 80% of the hair's structure and significantly influences hair characteristics. The cortex is a fibrous layer composed of fibrous keratin proteins.

[0004] The hair cuticle, which accounts for 10-15% of the hair's mass, is a multilayered structure composed of keratin, a hydrophobic protein, which surrounds the hair cortex in scale-like layers, providing protection. The hair cortex, a fibrous structure located between the hair medulla and the cuticle, accounts for 80-90% of the hair's mass and is composed of cortical cells and interstitial material, containing melanin and collagen proteins. It determines hair color, strength, and elasticity.

[0005] Because the hair epidermis is protected by a tough layer of keratin protein, it is generally resistant to heat and irritation. However, accumulated stress, such as strong physical stimuli, heat, repeated bleaching and dyeing, or prolonged exposure to UV rays, can oxidize hair proteins and cause the epidermis to split. Damaged hair has an irregular surface, is rough, lacks luster, and has low tensile strength, making it prone to breakage.

[0006] Probiotics are a general term for microorganisms that have beneficial effects on humans and animals. Most known probiotics are lactic acid bacteria. While probiotics have been reported to exert effective effects through various beneficial effects on the human body, research on the relationship between scalp or hair flora and the scalp or hair itself is limited.

[0007] Accordingly, there is a need to develop scalp-resident bacteria or hair-resident bacteria that can be useful for conditions related to hair or scalp.

[0008] One aspect provides a composition comprising a Bifidobacterium animalissubsp.lactis strain, a lysate thereof, a culture medium thereof, or an extract of the culture medium thereof.

[0009] Another aspect is to provide a use of the composition for preventing, improving or treating hair or scalp conditions.

[0010] Another aspect provides a method of preventing, improving, or treating a condition of a subject comprising administering the composition described above to the subject in need thereof.

[0011] One aspect provides a composition comprising a Bifidobacterium animalissubsp.lactis strain, a lysate thereof, a culture medium thereof, or an extract of the culture medium thereof.

[0012] The strain may be isolated. As used herein, the term "isolated" means something that is not found in nature but is artificially isolated and available. The strain may be isolated from dog skin. The strain may be isolated from dog keratinocytes. The strain may be a strain isolated by a method of inoculating dog keratinocytes onto Tryptic Soy Agar (TSA) and isolating and culturing a cultured colony for pure isolation.

[0013] The above strain may be a strain belonging to the genus Bifidobacterium (Bifidobacterium sp.).

[0014] The strain may comprise 16S rRNA having a sequence identity of about 95% or more, about 96% or more, about 97% or more, about 98% or more, about 99% or more, about 99.5% or more, about 99.9% or more, about 95% to 100%, about 96% to 100%, about 97% to 100%, about 98% to 100%, about 99% to 100%, about 99.5% to 100%, or about 99.9% to 100% with SEQ ID NO: 1. The strain may comprise 16S rRNA of SEQ ID NO: 1.

[0015] The term "sequence identity" as used herein refers to the degree of identity of amino acid residues or bases between sequences after aligning the two sequences to maximize the match in a specific comparison region. Sequence identity can be determined using methods known in the art. The percentage of sequence identity can be determined using known sequence comparison programs, such as BLASTN (NCBI), CLC Main Workbench (CLC bio), and MegAlignTM (DNASTAR Inc).

[0016] The above Bifidobacterium animalis subspecies lactis strain may be a strain deposited with the deposit number KCCM12993P. The strain deposited with the above deposit number may be the Bifidobacterium animalis subspecies lactis DS008 strain.

[0017] The above strain may have an effect of improving hair or scalp condition. For example, the improvement in hair or scalp condition may be at least one of strengthening hair roots, improving hair elasticity, improving hair texture, improving hair shine, improving damaged hair, or increasing hair thickness.

[0018] The term "lysate" may be used interchangeably with "lysate" in this specification, and may refer to a product obtained by breaking down the cell wall of a strain by chemical or physical force. The lysate may include the lysate itself, a concentrate thereof, or a lyophilized product thereof.

[0019] In this specification, the term "culture medium" may be used interchangeably with "culture supernatant", "conditioned medium" or "conditioned medium", and may mean the entire medium including the strain, its metabolites, extra nutrients, etc., obtained by culturing the strain for a certain period of time in a medium that can supply nutrients so that the Bifidobacterium animalis subspaces lactis strain can grow and survive in vitro. In addition, the culture medium may mean a culture medium obtained by removing the bacterial cells from the bacterial cell culture obtained by culturing the strain. Meanwhile, the liquid from the culture medium from which the bacterial cells have been removed is also called a "supernatant", and may be obtained by allowing the culture medium to stand still for a certain period of time and taking only the liquid in the upper layer excluding the portion that has settled to the lower layer, removing the bacterial cells through filtration, or centrifuging the culture medium to remove the sediment at the bottom and taking only the liquid in the upper layer. The "bacterial cells" above mean the strain itself of the present invention, and includes the strain itself selected by separating from a sample or the like, or the strain isolated from the culture medium by culturing the strain. The above-mentioned fungi can be obtained by centrifuging the culture solution and taking the portion that has settled to the lower layer, or by allowing the fungi to settle to the lower layer of the culture solution by gravity and then removing the upper liquid after leaving it still for a certain period of time.

[0020] The above culture solution may include the culture solution itself obtained by culturing the strain, a concentrate or lyophilized product thereof, or a culture supernatant obtained by removing the strain from the culture solution, a concentrate or lyophilized product thereof.

[0021] The culture medium and culture conditions for culturing the above Bifidobacterium animalis subspaces lactis strain can be appropriately selected or modified by those skilled in the art. For example, the culture solution may be obtained by culturing in a medium (e.g., R2A medium, RCM medium, or TSA medium) at a temperature above 10°C or below 40°C for a certain period of time, for example, 4 to 120 hours.

[0022] The term "culture solution extract" in this specification means an extract obtained from the culture solution or a concentrate thereof, and may include an extract, a diluted or concentrated extract, a dried product obtained by drying the extract, or a controlled or purified product thereof, or a fraction obtained by fractionating the same.

[0023] The above strain, its lysate, culture medium, or extract of the culture medium may be biologically pure. The above strain may be obtained by pure culture.

[0024]

[0025] Uses of the composition may include improving the condition of hair or scalp, or preventing or treating hair loss.

[0026] The improvement in the condition of the hair or scalp may be at least one of strengthening hair roots, improving hair elasticity, improving hair texture, improving hair shine, improving damaged hair, or increasing hair thickness.

[0027] The strain may increase the expression of keratin 10, one or more lipids associated with hair luster. The lipid associated with hair luster may be cholesterol. The strain may increase the thickness of one or more of the hair cuticle layer or the hair cortex layer.

[0028] “Strengthening hair roots” refers to all actions that strengthen and strengthen the roots of hair.

[0029] “Improving hair elasticity” may include increasing the tensile strength of the hair.

[0030] “Improving hair texture” or “improving hair shine” refers to any action that reduces split ends in the hair cuticle, increases hair shine, and smoothes out the curves of the hair surface.

[0031] “Damaged hair” may be hair that has lost its elasticity, texture, or shine due to strong physical stimulation or heat, repeated bleaching and dyeing, or long-term exposure to ultraviolet rays.

[0032] “Improvement” may mean any action that reduces at least the severity of a symptom, for example, a parameter related to the alleviation or treatment of a condition.

[0033] "Hair loss" refers to the loss of hair from the scalp or a condition in which hair becomes thicker or thinner. Hair loss can be caused by a decrease in the proportion of hair in the growth phase. Hair loss can be caused by exogenous or endogenous factors.

[0034] "Prevention" includes suppressing the occurrence of disease.

[0035] “Treatment” includes the inhibition, alleviation, or elimination of the development of a disease.

[0036] The composition according to one aspect may be for improving the condition of the hair or scalp of an animal. The animal may be a cow, horse, pig, dog, sheep, goat, or cat. The animal may be a pet dog or pet cat.

[0037] According to one aspect, a composition can exhibit an effect of improving the condition of hair or scalp by increasing the expression of keratin 10 and cholesterol and increasing the thickness of the hair cuticle layer and the hair cortex layer by including the Bifidobacterium animalis subspaces lactis strain, its lysate, culture medium, or extract of the culture medium.

[0038] In another specific example, the strain may be used together with another strain having an effect of improving hair or scalp condition, for example, a strain belonging to the genus Bifidobacterium, to produce a synergistic effect.

[0039] The composition comprises 0.0001 wt% to 99.9999 wt%, 0.001 wt% to 80 wt%, for example, 0.01 wt% to 60 wt%, 0.01 wt% to 40 wt%, 0.01 wt% to 30 wt%, 0.01 wt% to 20 wt%, 0.01 wt% to 10 wt%, 0.01 wt% to 5 wt%, 0.05 wt% to 60 wt%, 0.05 wt% to 40 wt%, 0.05 wt% to 30 wt%, 0.05 wt% to 20 wt%, 0.05 wt% to 10 wt%, 0.05 wt% to 5 wt%, 0.1 wt% to 60 wt%, 0.1 wt% to 40 wt%, 0.1 It may include the strain, its lysate, culture medium or extract of the culture medium in an amount of from 0.1 wt% to 30 wt%, from 0.1 wt% to 20 wt%, from 0.1 wt% to 10 wt%, or from 0.1 wt% to 5 wt%.

[0040] In this specification, the term "including as an active ingredient" means that the strain of this specification, its lysate, culture medium or extract of the culture medium is added to an extent that can exhibit the above-mentioned effect, and includes the formulation in various forms by adding various components as auxiliary components for drug delivery and stabilization, etc.

[0041] The composition may be in a liquid or dry state. In one embodiment, the composition may be in the form of a dry powder.

[0042] The drying method for preparing the above composition in a dry state may be any method commonly used in the art and is not particularly limited. Non-limiting examples of the drying method include air drying, natural drying, spray drying, and freeze drying. These methods may be used alone or in combination of at least two methods.

[0043] The composition may comprise an effective amount of an additive sufficient to reduce deterioration of the strain, its lysate, culture medium, or extract of the culture medium. The additive may be, for example, but is not limited to, a binder.

[0044] The composition may further comprise a cosmetically, pharmaceutically, or food additive acceptable carrier. The composition may be formulated with the carrier and provided as a cosmetic, pharmaceutical, or food additive.

[0045]

[0046] The above composition may be a cosmetic composition.

[0047] The above cosmetic composition may additionally include, in addition to the effective ingredients disclosed herein, components commonly used in cosmetic compositions, functional additives, etc., and may include, for example, conventional auxiliary agents such as antioxidants, stabilizers, solubilizers, surfactants, dispersants, emulsifiers, preservatives, vitamins, pigments, fragrances, etc., and carriers.

[0048] The above cosmetic composition is not particularly limited to a specific formulation, and the formulation may be appropriately selected depending on the intended purpose. The cosmetic composition may, for example, have a solubilized formulation, an emulsified formulation, or a dispersed formulation. The cosmetic composition may, for example, have a cosmetic formulation of an emollient toner, a nourishing toner, a massage cream, a nourishing cream, an essence, a pack, a gel, an ampoule, or a skin-adhesive type.

[0049] The above cosmetic composition may have any one formulation selected from hair mist, hair tonic, hair lotion, hair cream, hair spray, hair mousse, hair gel, hair conditioner, hair shampoo, hair rinse, hair pack, hair treatment, eyebrow tonic, eyelash tonic, or eyelash nutrient, or a pet formulation thereof.

[0050] In one specific example, the cosmetic composition may be in the form of a hair mist formulation.

[0051]

[0052] The above composition may be a composition for external use on the skin.

[0053] In the present specification, the external preparation for skin may be a cream, a gel, an ointment, a skin emulsifier, a skin suspension, a transdermal patch, a drug-containing bandage, a lotion, or a combination thereof. The external preparation for skin may be appropriately mixed with ingredients commonly used in external preparations for skin such as cosmetics or medicines, such as aqueous ingredients, oily ingredients, powder ingredients, alcohols, moisturizers, thickeners, ultraviolet absorbers, whitening agents, preservatives, antioxidants, surfactants, fragrances, coloring agents, various skin nutrients, or combinations thereof, as needed. The external preparation for skin may be a metal sequestering agent such as disodium edetate, trisodium edetate, sodium citrate, sodium polyphosphate, sodium metaphosphate, gluconic acid, etc.; a drug such as caffeine, tannin, bellapamil, licorice extract, glablidin, various herbal medicines, tocopherol acetate, glycyrrhizic acid, tranexamic acid, and derivatives or salts thereof; Vitamin C, magnesium ascorbyl phosphate, ascorbic acid glucoside, arbutin, kojic acid; sugars such as glucose, fructose, and trehalose can also be appropriately blended.

[0054]

[0055] The above composition may be a pharmaceutical composition.

[0056] The pharmaceutical composition may additionally comprise a pharmaceutically acceptable diluent or carrier. The diluent may be lactose, corn starch, soybean oil, microcrystalline cellulose, mannitol, or a combination thereof. The carrier may be an excipient, a disintegrant, a binder, a glidant, or a combination thereof. The excipient may be microcrystalline cellulose, lactose, low-substituted hydroxycellulose, or a combination thereof. The disintegrant may be calcium carboxymethylcellulose, sodium starch glycolate, calcium dihydrogen phosphate anhydrous, or a combination thereof. The binder may be polyvinylpyrrolidone, low-substituted hydroxypropyl cellulose, hydroxypropyl cellulose, or a combination thereof. The glidant may be magnesium stearate, silicon dioxide, talc, or a combination thereof.

[0057] The above pharmaceutical composition may be formulated as an oral or parenteral dosage form. Oral dosage forms may include granules, powders, liquids, tablets, capsules, dry syrups, etc. Parenteral dosage forms may include injections, ointments, etc.

[0058]

[0059] Another aspect provides a method of preventing, improving, or treating a condition of a subject comprising administering to the subject an effective amount of the above composition.

[0060] The condition of the above object may be a condition related to hair or scalp, a condition related to hair or scalp injury, or a condition related to hair or scalp inflammation.

[0061] As used herein, the terms "administering," "introducing," and "implanting" are used interchangeably and may refer to placement of a composition according to an embodiment into a subject by a method or route that results in at least partial localization of the composition to a desired site according to an embodiment.

[0062] Administration may be by any method known in the art. Administration may be administered directly to the subject by any means, including intravenous, intramuscular, oral, transdermal, mucosal, intranasal, intratracheal, or subcutaneous administration. Administration may be systemic or local.

[0063] The subject may be a mammal, such as a human, cow, horse, pig, dog, sheep, goat, or cat. The subject may be an individual in need of improvement in the condition of the hair or scalp, such as an individual in need of strengthening hair roots, improving hair elasticity, improving hair texture, improving hair shine, improving damaged hair, or increasing hair thickness.

[0064] The above administration is 0.1 mg to 1,000 mg per day per individual of the composition according to one specific example, for example, 0.1 mg to 500 mg, 0.1 mg to 100 mg, 0.1 mg to 50 mg, 0.1 mg to 25 mg, 1 mg to 1,000 mg, 1 mg to 500 mg, 1 mg to 100 mg, 1 mg to 50 mg, 1 mg to 25 mg, 5 mg to 1,000 mg, 5 mg to 500 mg, 5 mg to 100 mg, 5 mg to 50 mg, 5 mg to 25 mg, 10 mg to 1,000 mg, 10 mg to 500 mg, 10 mg to It may be administered at 100 mg, 10 mg to 50 mg, or 10 mg to 25 mg. However, the dosage may be prescribed in various ways depending on factors such as the formulation method, administration method, patient's age, weight, sex, pathological condition, food, administration time, administration route, excretion rate, and reaction sensitivity, and a person skilled in the art can appropriately adjust the dosage by considering these factors. The number of administrations may be once a day or twice or more within the range of clinically acceptable side effects, and the administration site may be administered at one or more sites, and the total number of administration days may be from 1 to 30 days per treatment at intervals of 2 to 5 days daily or every day. If necessary, the same treatment may be repeated after an appropriate period. For animals other than humans, the same dosage per kg as for humans may be administered, or the dosage may be converted to the above dosage based on the volume ratio (e.g., average value) of the organs (heart, etc.) of the target animal and humans.

[0065] According to a composition comprising a Bifidobacterium animalis subspaces lactis strain, a lysate thereof, a culture medium, or an extract of a culture medium according to an aspect thereof, since it has the effect of strengthening hair roots, improving hair elasticity, improving hair texture, improving hair shine, improving damaged hair, or increasing hair thickness, it can be applied in various ways for improving, preventing, or treating hair or scalp conditions, or hair loss.

[0066] Figure 1 shows the results showing the change in the expression level of keratin 10 when the cosmetic composition of Example 3 is applied to dog hair. a: Before treatment with the composition, b: After treatment with the composition.

[0067] Figure 2 shows the results showing the change in the expression level of hair gloss-related lipids when the cosmetic composition of Example 3 is applied to dog hair. a: Before treatment with the composition, b: After treatment with the composition.

[0068] Figure 3 is a graph quantitatively showing the change in thickness of the cuticle layer and cortex layer contained in hair when the cosmetic composition of Example 3 is applied to dog hair. a: Before treatment with the composition, b: After treatment with the composition.

[0069] The present invention will be described in more detail through the following examples. However, these examples are provided for illustrative purposes only and the scope of the present invention is not limited to these examples.

[0070]

[0071] Example 1: Isolation and identification of strains

[0072] Bifidobacterium animalis subspecies lactis strains were isolated and identified from the skin of healthy dogs.

[0073] Specifically, a sample, i.e., canine epidermal keratinocytes, obtained by washing the skin of a healthy dog ​​with sterile distilled water was inoculated onto Tryptic Soy Agar (BD) medium. After inoculation, the cells were cultured in a 28°C incubator for 48 hours, and 100 colonies formed were purified and cultured, which were then re-cultured in a 28°C incubator for 48 hours.

[0074] After culture, the colonies were subjected to 16S rRNA gene sequence identification. Primers designed to react only with bacteria were used for amplification. PCR amplification was performed for 30 cycles of 95°C for 1 minute, 55°C for 1 minute, and 75°C for 1 minute and 30 seconds, followed by a final extension at 72°C for 8 minutes and storage at 4°C. After the PCR reaction was completed, the DNA sequences of the isolated and cultured species were determined using an ABI-3730XL (ABI, USA).

[0075] The base sequence of the 16S rRNA region determined among the isolated and cultured microbial colonies was compared and analyzed with other registered strains using the BLAST program provided on the National Center for Biotechnology Information (NCBI) website. A novel microbial strain, Bifidobacterium animalissubsp.lactis (hereinafter referred to as “DS008”) with less than 99% homology was selected. The selected DS008 strain was deposited with the Korea Culture Collection for Microorganisms (KCCM) on May 28, 2021, and assigned the accession number KCCM12993P. The DS008 strain has a 16S rRNA sequence of sequence number 1 (complementary DNA).

[0076]

[0077] Example 2. Preparation of DS008 strain culture solution

[0078] The DS008 strain of Example 1 was inoculated into Reasoner's 2A (R2A) medium (Becton Dickinson, Cockeysville, MD) and cultured at 37°C under anaerobic conditions for 72 hours.

[0079]

[0080] Example 3. Preparation of a cosmetic composition containing a DS008 strain culture solution

[0081] A cosmetic composition containing a DS008 strain culture was prepared according to the respective ingredients and contents described in Table 1 below.

[0082] Ingredient content (weight%) Example 2 (DS008 strain culture solution) 1 Purified water 91.473 Butylene glycol 4 Glycerin 0.4 Cetrimonium chloride 0.087 Pentylene glycol 11,2-hexanediol 0.5 Ethylhexylglycerin 0.29 Citric acid 0.05 Tromethamine 0.1 Polysorbate 201 Flavoring agent 0.1 Total 100

[0083]

[0084] Specifically, purified water, butylene glycol, and glycerin described in Table 1 were mixed with cetrimonium chloride to prepare a mixture. Next, pentylene glycol, 1,2-hexanediol, and ethylhexylglycerin were added to the mixture, and the mixture was placed in an Agimixer and mixed to prepare a mixture. Next, citric acid and tromethamine were added to the mixture, and the mixture was placed in an Agimixer and mixed to prepare a mixture. Next, polysorbate 20 and a fragrance were mixed to the mixture, and the mixture was placed in an Agimixer and mixed to prepare a mixture. Next, the DS008 strain culture according to Example 2 was added to the mixture, and the mixture was placed in an Agimixer and mixed to prepare a cosmetic composition according to Example 3.

[0085]

[0086] Experimental Example 1. Analysis of hair texture improvement activity - Confirmation of increased keratin 10 expression.

[0087] To analyze the effect of DS008 strain culture on hair texture improvement activity, the expression of Keratin 10 was confirmed.

[0088] Specifically, the cosmetic composition according to Example 3 was applied to the back of a companion dog every day for two weeks, and the hair was collected on the last day of application. The collected hair was sectioned, fixed on a slide glass, and then subjected to keratin 10 immunohistochemistry staining.

[0089] The results are shown in Fig. 1. Fig. 1 shows a photograph of keratin 10 contained in dog hair taken with a fluorescence microscope when the cosmetic composition of Example 3 was applied to dog hair.

[0090] As shown in Fig. 1, the composition of Example 3 significantly increased the expression of keratin 10 in hair. These results indicate that the DS008 strain and a cosmetic composition containing the same can be useful for improving hair or scalp conditions and hair texture.

[0091]

[0092] Experimental Example 2. Analysis of hair gloss-improving activity - Confirmation of increased cholesterol expression in hair.

[0093] To analyze the effect of the DS008 strain culture on hair gloss improvement activity, the expression of hair gloss-related lipids was confirmed.

[0094] Specifically, the cosmetic composition according to Example 3 was applied to the dog's back every day for two weeks, and on the last day of application, the hair was collected and Nile red O staining was performed.

[0095] The results are shown in Fig. 2. Fig. 2 shows a photograph taken with a fluorescence microscope of cholesterol contained in dog hair when the cosmetic composition of Example 3 was applied to the dog hair.

[0096] As shown in Fig. 2, the composition of Example 3 significantly increased the expression of cholesterol in hair, a lipid associated with hair luster. These results indicate that the DS008 strain and a cosmetic composition comprising the same can be useful for improving hair or scalp condition and hair luster.

[0097]

[0098] Experimental Example 3. Analysis of activity to improve hair elasticity and increase hair thickness - Confirmation of increased thickness of hair cuticle and cortex layers.

[0099] To analyze the effect of DS008 strain culture on improving hair elasticity and increasing hair thickness, changes in the thickness of the cuticle and cortex layers of the hair were confirmed.

[0100] Specifically, the hair of a dog was treated with a cosmetic composition according to Example 3 in the same manner as in Experimental Example 1, and then keratin 10 immunohistochemical staining was performed. Then, the thickness of the cuticle layer and cortex layer of the hair was quantitatively analyzed using a fluorescence microscope.

[0101] The results are shown in Fig. 3. Fig. 3 is a graph quantitatively showing the thickness of the cuticle layer and cortex layer of hair when the composition of Example 3 is applied to dog hair.

[0102] As shown in Fig. 3, the composition of Example 3 significantly increased the thickness of the hair cuticle layer and the hair cortex layer. These results indicate that the DS008 strain and a cosmetic composition containing the same can be useful for improving hair or scalp conditions.

[0103]

[0104] In summary, it was confirmed that the Bifidobacterium animalis subspaces lactis DS008 strain or a composition containing it increases the expression of keratin 10 and cholesterol lipids related to hair luster, and increases the thickness of the hair cuticle layer and hair cortex layer. Therefore, it was found that the DS008 strain and a composition containing it have the effects of strengthening hair roots, improving hair elasticity, improving hair texture, improving hair luster, improving damaged hair, or increasing hair thickness.

[0105] The above description is merely an example of the technical idea of ​​the present invention, and those skilled in the art will appreciate that various modifications and variations can be made without departing from the essential characteristics of the present invention.

[0106]

Claims

1. A composition comprising a Bifidobacterium animalis subsp. lactis strain, a lysate thereof, a culture medium, or an extract of the culture medium.

2. A composition according to claim 1, wherein the composition is a cosmetic composition for improving hair or scalp condition.

3. A composition according to claim 1, wherein the strain is Bifidobacterium animalis subsp. lactis DS008 strain deposited under deposit number KCCM12993P.

4. A composition according to claim 2, wherein the improvement in the condition of the hair or scalp is at least one of strengthening hair roots, improving hair elasticity, improving hair texture, improving hair shine, improving damaged hair, or increasing hair thickness.

5. In claim 2, the composition increases the expression of at least one of keratin 10 and cholesterol.

6. A composition according to claim 2, which increases the thickness of at least one of a hair cuticle layer or a hair cortex layer.

7. A composition according to claim 2, wherein the composition is a cosmetic composition for improving the condition of hair or scalp of an animal.

8. A composition according to claim 7, wherein the animal is a pet dog or a pet cat.

9. A health functional food composition for improving hair or scalp condition, comprising a Bifidobacterium animalis subsp. lactis strain, a lysate thereof, a culture solution thereof, or an extract of the culture solution thereof.

10. A pharmaceutical composition for preventing or treating hair loss, comprising a Bifidobacterium animalis subsp. lactis strain, a lysate thereof, a culture medium, or an extract of a culture medium as an active ingredient.

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