Methods and compositions for altering seed characteristics in oilseed plants

Abstract

Mutant oilseed plants that exhibit increased seed size, increased seed oil content, and / or increased seed protein content are described herein.

Description

[0001] METHODS AND COMPOSITIONS FOR ALTERING SEED CHARACTERISTICS IN OILSEED PLANTS

[0002] CROSS REFERENCE TO RELATED APPLICATIONS

[0003] This application claims the benefit of priority under 35 U.S. C. § 119(e) to U.S. Application No. 63 / 525,553 filed July 7, 2023.

[0004] FEDERALLY SPONSORED RESEARCH OR DEVELOPMENT

[0005] This invention was made with government support under 2019-67009-29004 awarded by US Department of Agriculture, National Institute of Food and Agriculture, under 2019-69012- 29851 awarded by US Department of Agriculture, Agriculture and Food Research Initiative, and under DE-SC0021286 awarded by Department of Energy, Biological and Environmental Research. The government has certain rights in the invention.

[0006] TECHNICAL FIELD

[0007] This disclosure generally relates to plant biotechnology, and, more specifically, methods and compositions for increasing seed size in oil seed plants.

[0008] BACKGROUND

[0009] Pennycress (Thlapsi arvense L., Field Pennycress) is being developed as a winter annual oilseed cash cover crop for the U.S. Midwest Com Belt owing to its extreme cold tolerance, high seed yields, and relatively short life cycle. In the southern U.S. Midwest, pennycress is being planted after corn or soybeans as a winter cover crop with ecological benefits including reducing nutrients runoff. Varieties have been bred to produce over 1,500 pounds of seed per acre, yielding about 65 gallons of oil and 1,200 pounds of meal per acre.

[0010] SUMMARY

[0011] This document describes mutant oilseed plants that exhibit increased seed size, increased seed oil content, and / or increased seed protein content. Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which the methods and compositions of matter belong. Although methods and materials similar or equivalent to those described herein can be used in the practice or testing of the methods and compositions of matter, suitable methods and materials are described below. In addition, the materials, methods, and examples are illustrative only and not intended to be limiting. All publications, patent applications, patents, and other references mentioned herein are incorporated by reference in their entirety.

[0012] In one aspect, a non-naturally occurring mutant oilseed plant is provided that exhibits increased plant size, increased seed size, increased seed weight, increased seed oil content, and / or increased seed protein content. Such an oilseed plant typically includes a loss-of-function mutation in a dal nucleic acid sequence (e.g., in a sequence having at least 95% sequence identity to the sequence shown in SEQ ID NO: 1); a loss-of-function mutation in a darl nucleic acid sequence (e.g., in a sequence having at least 95% sequence identity to the sequence shown in SEQ ID NO:3); a loss-of-function mutation in a upl3 nucleic acid sequence (e.g., in a sequence having at least 95% sequence identity to the sequence shown in SEQ ID NO: 5); and / or a loss-of-function mutation in a da2 nucleic acid sequence (e.g., in a sequence having at least 95% sequence identity to the sequence shown in SEQ ID NO:7). Such a mutant oilseed plant exhibits increased plant size, increased seed size, increased seed weight, increased seed oil content, and / or increased seed protein content relative to a corresponding oilseed plant lacking the loss-of-function mutation(s).

[0013] Representative mutant dal, darl, upl3 and da2 sequences (nucleic acid and encoded polypeptide) made using CRISPR are shown in SEQ ID NO:9-36.

[0014] Representative mutant dal, darl, upl3 and da2 sequences (nucleic acid and encoded polypeptide) made using EMS mutagenesis are shown in SEQ ID NO:37-72.

[0015] In some embodiments, the oilseed plant exhibits at least a 5% (e.g., at least 5% to 30%) increase in height and / or organ size (e.g., leaves, stems, roots, flowers, and / or seed pods) relative to an oilseed plant lacking the loss-of-function mutation(s). In some embodiments, the oilseed plant exhibits at least a 10% (e.g., at least 10% to 60%) increase in seed size relative to an oilseed plant lacking the loss-of-function mutation(s). In some embodiments, the oilseed plant exhibits at least a 10% (e.g., at least 10% to 60%) increase in seed weight relative to an oilseed plant lacking the loss-of-function mutation(s). In some embodiments, the oilseed plant exhibits at least a 5% (e.g., at least 5% to 70%) increase in the amount of seed oil content relative to a oilseed plant lacking the loss-of-function mutation(s). In some embodiments, the oilseed plant exhibits at least a 5% (e g., at least 5% to 95%) increase in the amount of seed protein content relative to an oilseed plant lacking the loss-of-function mutation(s).

[0016] In some embodiments, the oilseed plant further comprises a mutation in transparent testa8 (tt8). In some embodiments, the oilseed plant further comprises a mutation in a fatty acid elongase 1 (faelf nucleic acid. In some embodiments, the oilseed plant further comprises a mutation in tt8 and in fael.

[0017] In some embodiments, the oilseed plant is selected from pennycress, rapeseed, soybean, sunflower, canola, flax, camelina, carinata, crambe, and lepidium plants. In some embodiments, the mutation is selected from a point mutation, a deletion, an insertion, a duplication, and an inversion.

[0018] In another aspect, seed produced by an oilseed plant described herein is provided, wherein the seed comprises at least one of the loss-of-function mutation(s).

[0019] In still another aspect, methods of making a non-naturally occurring mutant oilseed plant exhibiting increased plant size, increased seed size, increased seed weight, increased seed oil content, and / or increased seed protein content are provided. Such methods typically include mutating cells from an oilseed plant; regenerating mutant oilseed plants from the mutated cells; and selecting mutant oilseed plants that include a loss-of-function mutation in a dal nucleic acid sequence (e.g., in a sequence having at least 95% sequence identity to the sequence shown in SEQ ID NO: 1); a loss-of-function mutation in a darl nucleic acid sequence (e.g., in a sequence having at least 95% sequence identity to the sequence shown in SEQ ID NO:3); a loss-of- function mutation in a upl3 nucleic acid sequence (e.g., in a sequence having at least 95% sequence identity to the sequence shown in SEQ ID NO:5), and / or a loss-of-function mutation in a da2 nucleic acid sequence (e.g., in a sequence having at least 95% sequence identity to the sequence shown in SEQ ID NO:7), thereby making an oilseed plant that exhibits increased plant size, increased seed size, increased seed weight, increased seed oil content, and / or increased seed protein content relative to a corresponding oilseed plant lacking the loss-of-function mutation(s).

[0020] In some embodiments, the loss-of-function mutations are introduced using EMS, CRISPR, TALEN, Zine-finger nuclease, or a combination thereof. In some embodiments, the mutation is selected from a point mutation, a deletion, an insertion, a duplication, and an inversion.

[0021] In still another aspect, methods of planting the oilseed seeds described herein are provided. In some embodiments, the oilseed seed can be planted at a depth of up to 1.5 inches.

[0022] In another aspect, methods for eliminating the expression of DAI, DARI, DA2 and / or UPL3 in an oilseed plant are provided. Such methods typically include: expressing a transgene encoding an inhibitory RNA molecule that inhibits expression of the DAI, DARI, UPL3, and / or DA2 in the oilseed plant, wherein the inhibitory RNA molecule comprises at least 25 consecutive nucleotides that have 91% or greater sequence identity to SEQ ID NO:1, 3, 5, and / or 7, wherein expression of the DAI, DARI, DA2, and / or UPL3 is eliminated in the oilseed plant relative to an oilseed plant lacking the transgene.

[0023] In still another aspect, methods for producing a mutant oilseed plant are provided. Such methods typically include the steps of a) providing a first oilseed plant and a second oilseed plant, the first oilseed plant comprising a mutation in a dal, darl, upl3, and / or da2 nucleic acid sequence having a non-mutant sequence as set forth in SEQ ID NOs: l, 3, 5, and / or 7, wherein the first oilseed plant exhibits a phenotype of increased seed size, increased seed oil content, and / or increased seed protein content and the second oilseed plant exhibits a desired phenotypic trait; b) crossing the first oilseed plant with the second oilseed plant to produce one or more Fi progeny plants; c) collecting seed produced by the Fi progeny plants; and d) germinating the seed to produce oilseed plants having a phenotype of increased plant size, increased seed size, increased seed weight, increased seed oil content, and / or increased seed protein content.

[0024] In one aspect, non-naturally occurring mutant oilseed plants exhibiting increased plant size, increased seed size, increased seed weight, increased seed oil content, and / or increased seed protein content are provided. Such mutant oilseed plants typically include a loss-of-function mutation in a dal nucleic acid sequence, wherein the non-mutant dal nucleic acid sequence has at least 95% sequence identity to the sequence shown in SEQ ID NO: 1; a loss-of-function mutation in a darl nucleic acid sequence, wherein the non-mutant darl nucleic acid sequence has at least 95% sequence identity to the sequence shown in SEQ ID NO:3; a loss-of-function mutation in a upl3 nucleic acid sequence, wherein the non-mutant upl3 nucleic acid sequence has at least 95% sequence identity to the sequence shown in SEQ ID NO:5; and / or a loss-of-function mutation in a da2 nucleic acid sequence, wherein the non-mutant da2 nucleic acid sequence has at least 95% sequence identity to the sequence shown in SEQ ID NO: 7. Generally, the mutant oilseed plant exhibits increased plant size, increased seed size, increased seed weight, increased seed oil content, and / or increased seed protein content relative to a corresponding oilseed plant lacking the loss-of- function mutation(s).

[0025] In some embodiments, the mutant dal nucleic acid sequence is selected from SEQ ID NOs: 9, 11, 13, 37, 39, 41, 21, 73, 77, 81, 83, 89, 93, 97, 107, and 111. In some embodiments, the mutant darl nucleic acid sequence is selected from SEQ ID NOs: 15, 17, 19, 51, 47, 49, 53, 55, 22, 75, 79, 85, 87, 91, 95, 99, and 113. In some embodiments, the mutant upl3 nucleic acid sequence is selected from SEQ ID NOs: 25, 27, 29, 65, 57, 59, 61, 63, 67, 69, 71, 101, 103, 105, 109, and 115. In some embodiments, the mutant dal nucleic acid sequence is selected from SEQ ID NOs: 31, 33, 35, 43, 45, 117, 119, 121, 123, 125, 127, 129, 131, 133, and 135.

[0026] In some embodiments, the oilseed plant exhibits at least a 5% (e.g., at least 5% to 30%) increase in height and / or organ size (e.g., leaves, stems, roots, flowers, and / or seed pods) relative to an oilseed plant lacking the loss-of-frinction mutation(s). In some embodiments, the oilseed plant exhibits at least a 10% (e.g., at least 10% to 60%) increase in seed size relative to an oilseed plant lacking the loss-of-frinction mutation(s). In some embodiments, the oilseed plant exhibits at least a 10% (e.g., at least 10% to 60%) increase in seed weight relative to an oilseed plant lacking the loss-of-function mutation(s). In some embodiments, the oilseed plant exhibits at least a 5% (e g., at least 5% to 70%) increase in the amount of seed oil content relative to an oilseed plant lacking the loss-of-function mutation(s). In some embodiments, the oilseed plant exhibits at least a 5% (e.g., at least 5% to 95%) increase in the amount of seed protein content relative to an oilseed plant lacking the loss-of-function mutation(s).

[0027] In some embodiments, the oilseed plant further comprises a mutation in transparent testa8 (tt8). In some embodiments, the oilseed plant further comprises a mutation in a fatty acid elongase 1 {fael) nucleic acid. In some embodiments, the oilseed plant further comprises a mutation in tt8 and in fael.

[0028] In some embodiments, the oilseed plant is selected from pennycress, rapeseed, soybean, sunflower, canola, flax, camelina, carinata, crambe, and lepidium plants. In some embodiments, the mutation is selected from a point mutation, a deletion, an insertion, a duplication, and an inversion. In another aspect, seed produced by any of the mutant oilseed plants described herein, wherein the seed comprises at least one of the loss-of-fimction mutation(s).

[0029] In still another aspect, methods of making a non-naturally occurring mutant oilseed plant exhibiting increased plant size, increased seed size, increased seed weight, increased seed oil content, and / or increased seed protein content are provided. Such methods typically include mutating cells from an oilseed plant; regenerating mutant oilseed plants from the mutated cells; and selecting mutant oilseed plants comprising a loss-of-function mutation in a dal nucleic acid sequence, wherein the dal nucleic acid sequence has at least 95% sequence identity to the sequence shown in SEQ ID NO: 1; a loss-of-function mutation in a darl nucleic acid sequence, wherein the darl nucleic acid sequence has at least 95% sequence identity to the sequence shown in SEQ ID NO:3; a loss-of-function mutation in a upl3 nucleic acid sequence, wherein the upl3 nucleic acid sequence has at least 95% sequence identity to the sequence shown in SEQ ID NO:5, and / or a loss-of-function mutation in a da2 nucleic acid sequence, wherein the da2 nucleic acid sequence has at least 95% sequence identity to the sequence shown in SEQ ID NO: 7; thereby making an oilseed plant that exhibits increased plant size, increased seed size, increased seed weight, increased seed oil content, and / or increased seed protein content relative to a corresponding oilseed plant lacking the loss-of-function mutation(s).

[0030] In some embodiments, the loss-of-function mutations are introduced using EMS, CRISPR, TALEN, Zine-finger nuclease, or a combination thereof. In some embodiments, the mutation is selected from a point mutation, a deletion, an insertion, a duplication, and an inversion.

[0031] In another aspect, methods of planting the mutant oilseed seed described herein is provided. In some embodiments, the oilseed seed can be planted at a depth of up to 1.5 inches.

[0032] In still another aspect, methods for inhibiting the expression of dal, darl, upl3 and / or da2 in an oilseed plant are provided. Such methods typically include expressing at least one transgene encoding an inhibitory RNA molecule that inhibits expression of the dal, darl, upl3 and / or da2 in the oilseed plant, wherein the inhibitory RNA molecule comprises at least 25 consecutive nucleotides that have 90% or greater sequence identity to SEQ ID NO:1, 3, 5, and / or 7, wherein expression of the dal, darl, upl3 and / or da2 is inhibited in the oilseed plant relative to an oilseed plant lacking the at least one transgene. In yet another aspect, methods for producing a mutant oilseed plant are provided. Such methods typically include the steps of: a) providing a first oilseed plant and a second oilseed plant, the first oilseed plant comprising a mutation in a dal, darl, upl3, and / or da2 nucleic acid sequence having a non-mutant sequence as set forth in SEQ ID NOs: l, 3, 5, and / or 7, wherein the first oilseed plant exhibits a phenotype of increased seed size, increased seed oil content, and / or increased seed protein content and the second oilseed plant exhibits a desired phenotypic trait; b) crossing the first oilseed plant with the second oilseed plant to produce one or more Fi progeny plants; c) collecting seed produced by the Fi progeny plants; and d) germinating the seed to produce oilseed plants having a phenotype of increased plant size, increased seed size, increased seed weight, increased seed oil content, and / or increased seed protein content.

[0033] DESCRIPTION OF THE DRAWINGS

[0034] FIG. 1 is a schematic showing how guide RNAs that target both DAI and DARI gene were designed.

[0035] FIGs. 2A-2I show plant morphology observed in upl3 and upl3tt8fael from T3 generation as compared to WT (Spring32) and control tt8fael in chamber conditions (hood room) where, upl3-l, upl3-2, and upl3-3 represent three independent pennycress mutant alleles harboring homozygous mutations in UPL3 gene. (upl3tt8fael)-l, (upl3tt8fael)-2, and (upl3tt8fael)'-3 represent three independent homozygous mutations in UPL3 gene, in a pennycress domesticated background containing an +A insertion in the TT8 gene conferring reduced seed coat fiber and tannins and a -4bp deletion in the FAE1 gene conferring greatly reduced very long chain fatty acids including erucic acid in the seed oil. 2A) and 2E) Plant height of mutants compared to wild type at flowering stage; 2B) and 2F) Final plant height of mutants and wild type at senesced stage; 2C) and 2G) Bigger pods of mutants vs. wild type. 2D) and 2H) Bigger seeds in mutants vs. control under microscopic observation (Zoom 45X) I) Bigger Flowers in mutants vs. control. FIGs. 2A-2D: upl3 vs. wild type, FIGs. 2E-2I: upl3tt8fael mutants vs. control tt8fael.

[0036] FIGs. 3A-3D show plant growth and seed size comparisons of upl3 compared to wild type (Spring 32) in chamber conditions. Graph showing upl3 mutants grown in the hood room chamber from T3 generation as compared to WT (Spring32) where, upl3-l, upl3-2 and upl3-3 represents three independent homozygous mutations in UPL3 gene. 3A) Seed size measurements, N=1000-1200 seeds; 3B) Thousand Grain Weight (gm); 3C) Number of seeds per pod in an individual plant; 3D) Plant Height (cm). Error bars represents standard deviations of the means. Different letters represent statistical differences (p < 0.05) as determined by one-way ANOVA and Tukey’s test, N= 5 plants for 3A, 3B, 3D; N=15 pods for 3C.

[0037] FIG. 4 shows that dal, darl, and upl3 mutations increase seed size. Different letters in all graphs represent statistical differences (p < 0.05) as determined by one-way ANOVA and Tukey’s test with six biological replicates. Percent represent differences from the wild-type (WT) and tt8 fael controls. N= 800-1000 seeds.

[0038] FIG. 5A-5D shows plant growth and seed size comparisons of upl3tt8fael mutants and control tt8fael in Spring 32 background in two rounds of experiments in chamber conditions. upl3tt8fael-l, upl3tt8fael-2, and up!3tt8fael-3 represents three independent homozygous mutation in UPL3 gene, +A in TT8 gene and -4bp in FAE1 gene. 5A) Seed size measurements, N=1000-1200 seeds; 5B) Thousand Grain Weight (gm); 5C) Number of seeds per pod in individual plant; 5D) Plant height (cm). Error bars represents standard deviation of the mean. Different letters represent statistical differences (p < 0.05) as determined by one-way ANOVA and Tukey’s test, N= 5 plants for 5A, 5B, 5D; N=15 pods for 5C.

[0039] FIG. 6 shows that dal, darl, and upl3 mutations increase seed weight.

[0040] FIG. 7 shows that dal and darl mutations but not upl3 mutations increase seed oil content. Determined by pulsed-field Nuclear Magnetic Resonance (NMR).

[0041] FIG. 8 shows that dal and darl mutations but not upl3 mutations increase seed oil content.

[0042] FIG. 9 shows that upl3 mutations increase oleic acid and reduce erucic acid seed oil content.

[0043] FIG. 10 shows that pennycress upl3 mutant seeds contain glucosinolate amounts equal to or higher than wild type. Determined using Near Infrared (NIR) Spectroscopy as described in Chopra et al., (2019).

[0044] FIGs. 11A-1 IF show plant morphology observed in CRISPR generated mutants of dal, darl, daldarl mutants compared to wildtype (WT) grown in chamber (New). 11 A) Plant height at flowering stage; 1 IB) Final plant height at senesced stage; 11C) Bigger mutant leaves; 1 ID) Bigger mutant flowers; 1 IE) Bigger mutant seeds; 1 IF) Bigger seed pods in mutants.

[0045] FIGs. 12A-12D show plant growth and seed size comparisons of dal, darl, daldarl with wild type (WT) (Spring 32) grown in growth chamber conditions, dal-1, dal-2, dal-3 are three independent homozygous mutants in DAI gene, darl-1, darl -2, darl-3 represents three independent homozygous mutations in DARI gene whereas, (daldarl)-l, (daldarl)-2, (daldarl)-3 are three independent homozygous mutations in both DAI and DARI. 12A) Seed size measurements, N=300-900 seeds; 12B) Thousand Grain Weight (gm) of each plant; 12C) Number of seeds per pod; 12D) Individual plant height. Error bars represent standard deviation of the mean with six biological replicates. Different letters represent statistical differences (p < 0.05) as determined by one-way ANOVA and Tukey’s test. ** represent statistical differences (p < 0.01) as determined by one-way ANOVA and Tukey’s test. N= 6 plants for 12A, 12B, 12D; N=15 pods for 12C.

[0046] FIG. 13 shows that dal, darl, and upl3 mutations increase seed sizes in the tt8 fael double mutant background.

[0047] FIG. 14 shows total oil content in control (tl8fae 1) vs. upl3tt8fael mutants in Spring32 background in two different experiments in growth chamber conditions. Graph showing Pulse Field NMR Analysis for Average Total Oil (DWB) in wildtype and CRISPR induced upl3tt8fael mutants from T4 generation as compared to control tt8fael where, (upl3tt8fael)-l, (upl3tt8fael)~ 2, and (upl3tt8fael)-3 represents three independent homozygous mutation in UPL3 gene, +A in TT8 gene and -4bp in FAE1 gene. Error bars represent standard deviations of the means with four biological replicates. N=0.75 gm of seeds. ** represent statistical differences (p < 0.01) as determined by one-way ANOVA and Tukey’s test.

[0048] FIG. 15 shows pennycress mutants and wild type plants at mature stage. Genotypes are listed below the pictures. See also FIG. 11.

[0049] FIG. 16 shows pennycress mutants and wild type plants at mature stage. Genotypes are listed below the pictures.

[0050] FIG. 17 show dal darl double mutant, daldarltt8fael quadruple mutant, upl3 single mutant, upl3tt8fael triple mutant, dalupl3 double and daldarlupl3 triple mutants have visibly bigger pods compared to controls: wild type (WT) and tt8fael double mutant (domestication traits background). See also FIG. 11.

[0051] FIG. 18 show da2dal double mutant, da2daldarl triple mutant, da2daltt8fael quadruple mutant, and da2daldar ltt8fae 1 quintuple mutant plants have visibly bigger pods compared to controls: wild type (WT) and tl8fael double mutant (domestication traits background). FIG. 19A shows images of seeds from the various pennycress mutants (single, double, and triple mutant combinations) compared to wild type (Spring32-10). Mutant names are above the pictures. The scale at the top of each picture has 1 mm increments.

[0052] FIG. 19B shows images of seeds from the various pennycress mutants (single, double, and triple mutant combinations) in the tt8 fael domestication traits background. Mutant names are above the pictures. The scale at the top of each picture has 1 mm increments.

[0053] FIG. 20 shows that dal, darl, upl3, and da2 mutants and mutant combinations germinate normally and have normal root and shoot growth phenotypes on vertically positioned 0.8% agar ’A strength MS plates (no sucrose). Grid on plates is 1 cm squared.

[0054] FIG. 21 shows that da2 mutants and mutant combinations have increased weight of oil per seed compared to wild type (WT Spring32) and tt8 fael (domestication traits background) controls. Determined by pulsed-field NMR.

[0055] FIG. 22 shows that fatty acid composition of seed oil for the different mutants compared to controls. The fael knockout mutation greatly reduces or abolishes C20-1, C22-0, C22-1 (erucic acid), and C24-0 very long chain fatty acids in the seed oil. Bars are standard deviation.

[0056] FIG. 23 shows that the seeds of pennycress da2 mutants and mutant combinations have increased seed length, width and area compared to the wild-type Spring 32-10 control (WT Spring32). n=7-8 biological reps; 800-1000 seeds per rep.

[0057] FIG. 24 shows that the seeds of pennycress da2 mutants and mutant combinations have increased seed length, width and area compared to the tt8 fael double mutant domesticated control. n=7-8 biological reps; 800-1000 seeds per rep.

[0058] FIG. 25 shows that the seeds of pennycress da2 mutants and mutant combinations weigh more than wild-type (WT Spring32) and tt8 fael double mutant domesticated seeds. TGW = thousand grain weight = weight per 1,000 seeds. n=7-8 biological reps; 800-1000 seeds per rep.

[0059] FIG. 26 shows that total seed yield of all mutant lines except for the da2 dal darl tt8 fael quintuple mutant did not differ significantly from wild-type (WT Spring32) and tt8 fael double mutant domesticated plants. n=7-8 biological reps.

[0060] FIG. 27 shows that seeds of dal mutants and daldarl mutant combinations contain markedly more oil per seed than controls (wild type and tt8fael') whereas upl3 mutants and mutant combinations have marginally more oil (in most cases, not significant). Note these data are from a separate grow out from that shown in FIGs. 2 - 14. Determined by pulsed-field NMR. FIG. 28 shows that seeds of all single mutants and mutant combinations with the exception of darl were significantly larger than wild type. Note these data are from a separate grow out from that shown in FIGs. 2 - 14. n=7-8 biological reps; 800-1000 seeds per rep.

[0061] FIG. 29 shows that seeds of all mutants in the tt8fael domesticated background with the exception of darl were significantly larger than tt8fael control. Note these data are from a separate grow out from that shown in FIGs. 2 - 14. n=7-8 biological reps; 800-1000 seeds per rep.

[0062] FIG. 30 shows that seeds of dal and daldarl mutant combinations were significantly heavier than controls (wild type and ttSfaeF) whereas darl single mutant seed weights were generally no different from controls. Note these data are from a separate grow out from that shown in FIGs. 2 - 14. n=7-8 biological reps.

[0063] FIG. 31 shows that total seed yields per plant were, generally, not significantly different between any of the mutants and controls. Note these data are from a separate grow out from that shown in FIGs. 2 - 14. n=7-8 biological reps.

[0064] DETAILED DESCRIPTION

[0065] While much progress has been made employing modern genetic techniques to improve pennycress agronomic traits leading to commercial launch of CoverCress™ as a new intermediate oilseed crop (Chopra et al., 2018; Chopra et al., 2020; McGinn et al., 2019), crucial work remains in improving additional traits including the size of penny cress seeds. Larger seed size is an important agronomic trait in crop domestication (Meyer et al., 2012). Pennycress seeds in most natural populations weigh only about 1 mg with an area of less than 1.8 square mm. This small seed size limits the depth at which the seeds can be planted as well as poses challenges with seed harvesting and handling in that the seeds are difficult to separate from chaff in combines and easily slip through small openings in farm machinery and storage bins. Moreover, larger seeds can potentially have higher oil and / or protein content, being more economical to transport and process.

[0066] An association between seed size and / or seed weight and germinability and vigor has been widely studied in a variety of species (Li and Li, 2015). Bigger seeds tend to accumulate more nourishing substances to support germination and seedling establishment. Moreover, larger seeds are more likely to remain viable for a longer period in dormancy. It was shown that increased seed size in cabbage and turnips corresponded with significantly improved germination and seedling vigor (Hanumaiah and Andrews, 1973); seedlings grew more rapidly and produced larger plants having higher yields compared to those originating from the small seeds. Work by Smith and Camper (1975) suggested that soybean plants originating from larger seeds were taller throughout the growing season and outyielded plants from smaller seeds (Smith and Camper Jr, 1975). Relatively larger seeds have also been shown to possess higher tolerances to abiotic stresses, whereas small seeds (like pennycress seeds) are more efficient at dispersing over wider areas (Moles et al., 2005; Westoby et al., 2002).

[0067] Seeds are heterotrophic organs derived from double fertilization, consisting of three different genetic compositions: the diploid embryo (zygotic tissue), triploid endosperm (zygotic tissue), and diploid seed coat (maternal tissue) (Li, Song, et al., 2019). During double fertilization, one of the two sperm cells fuses with an egg cell to form the diploid embryo. The diploid central cell is fertilized by the other sperm cell to form a triploid endosperm (Li, Xu, et al., 2019). Upon fertilization, the maternal integuments surrounding the developing embryo and endosperm undergo cell differentiation and accumulate pigments, mucilage, starch granules, and secondary cell wall components to form the seed coat (Beeckman et al., 2000) (Windsor et al., 2000).

[0068] The final size of the seed is affected by the growth of the integuments which is determined by the maternal genotype (Li, Xu, et al., 2019). Various studies in Arabidopsis and rice have determined that transcriptional regulation and protein ubiquitination along with G- protein, MAPK, and hormone signaling are involved in maternal control of seed size (Li, Xu, et al., 2019). Studies of the Arabidopsis haiku 1, haiku2, and miniseedS mutants have also shown that aberrant endosperm development correlated with smaller seed size. Pollination of these mutants with wild-type pollen resulted in seeds of wild-type size, whereas in other instances maternal effects on seed size have been observed (Garcia et al., 2003) (Li and Li, 2015). Therefore, it remains unclear how the endosperm, embryo, and integument coordinately act to regulate seed size in plants.

[0069] Pennycress seeds are comprised of an embryo that is rich in oil and protein (34% and 20% by seed weight, respectively) surrounded by a thick fiber-rich seed coat that is resistant to degradation. In most dicots like Arabidopsis thaliana and pennycress, the endosperm nourishes the developing embryo and eventually gets consumed, leaving the embryo and seed coat as the seed components. By contrast, in monocots like rice, wheat and corn, endosperm comprises a major portion of the mature seed providing energy to the seedling upon germination (Li, Song, et al., 2019). MALDLMS spatial imaging analyses of phosphatidylcholine (PC) and triacylglycerol (TAG) molecular species in wild-type penny cress embryo sections from mature seeds revealed that erucic acid (22C: 1) within TAG is highly enriched in cotyledons. Cotyledons serve as storage organs and the erucic acid-rich TAG likely provides energy for the germinating seedling (Jarvis et al., 2021). In contrast, polyunsaturated fatty acids (mostly 18C:2 and 18C:3) were found to be enriched in pennycress embryonic axis tissues which may be utilized for cellular membrane expansion during seed germination and seedling emergence (Claver et al., 2020; Goffman et al., 2005) (Tsogtbaatar et al., 2020).

[0070] Brassica seed coats contain structural polysaccharide and phenolic polymers including cellulose, hemicellulose, lignin, condensed tannins and secondary metabolites that protect the embryo from several biotic and abiotic challenges and play a role in controlling seed dormancy (Debeaujon et al., 2000). Brassica seed coats (including that of penny cress) are comprised of an outer and inner integument. The inner integument contains three cell layers, the innermost of which is called the endothelium, or iil layer which has an important function of producing proanthocyanidins (PAs). PAs are phenolic compounds that get polymerized in part by oxidation into condensed tannins. Condensed tannins are dark brown colored and act as defense compounds against pathogens and pests and are also thought to protect against abiotic stress such as ultraviolet light (Lepiniec et al., 2006). In rapeseed seeds, condensed tannins comprise as much as 6% of the seed coat (LIP§A et al., 2009). So-called transparent testa (tt) mutants lack condensed tannins in their seed coats, which results in the seeds being tan, yellow, or “golden” in color (Chopra et al., 2018). Of interest, CRISPR-induced mutations first generated in our lab in the penny cress tt8 gene (Esfahanian, 2021 thesis) confer the reduced seed coat fiber trait which is incorporated in the new crop CoverCress™.

[0071] Although seed mass within an individual species was considered to be relatively constant, studies have demonstrated that seed size varies for given species among sites, reflective of environmental effects (Harper, 1977; Wulff, 1986). However, studies have shown that genetic effects largely underly differences in seed size / mass variations (Delouche, 1980) (Wulff, 1986). Regarding environmental effects, soil fertility, abiotic stressors such as drought, fecundity, timing of flowering, sibling rivalry and position of seeds within a plant all affect seed size. Seed obtained from environments with relatively higher macronutrient concentrations are often heavier and contain higher levels of mineral nutrients like nitrogen and phosphorus (Obeso, 2012). A study by Oj ala. found that seed mass and germination percentage of Angelica archangelica were higher in seeds on the primary umbel than in those on secondary, tertiary, and quaternary umbels (Ojala, 1985). Hendrix suggested that the production of smaller seeds on later-developing umbels might be due to the decrease in the amounts of available resources over the growth season, which is disadvantageous for later-developing umbels as they compete for limited resources (Hendrix, 1984). In contrast, for single seeded fruits, several studies have also discovered no direct relationship between fruit number and fruit or seed weight suggesting that a resource-limited trade-off rarely occurs between fruits (Primack, 1978). In addition, seed size is also negatively associated with the number of seeds produced by a plant because of the limited resources of the mother plant (Harper et al., 1970) (Jakobsson and Eriksson, 2000). Larger seeds might give rise to more successful individuals than those arising from small seeds (Boyd et al., 1990). Increased seed size tends to reduce seed number as long as the pod size remains the same. Nevertheless, the simultaneous selection for large seeds and large pods may permit the seed number to remain high along with increment in seed size (Tedin, 1925).

[0072] Several signaling pathways have been found to control seed size. However, these genetic pathways are not fully elucidated. In short, how plants know and determine their seed size is complex and not entirely clear. Moreover, while pathways may be shared by different plant species, there are functional differences leading to those species having drastically different organ sizes, organ shapes, and seed sizes, which is a testament to the function of a gene and / or pathway in one species not predicting function and / or effect in another species.

[0073] To test what genetic changes could increase seed size, result in higher amounts of oil and / or protein content, bigger organ sizes, higher yields, etc. in pennycress, we employed the genome editing technique, CRISPR / Cas9 as well as ethyl methane sulfonate (EMS) chemical mutagenesis forward and reverse genetic approaches. CRISPR / Cas9 genome editing constitutes a predominantly reverse genetics approach that involves designing guide RNA (gRNA) “protospacers” of about 19-20 nucleotides complementary to the DNA sequences within the target gene. The construct containing the specific guide RNAs are transformed into the plant along with the gene Cas9 endonuclease isolated from, e.g., the bacteria, Streptococcus pyogenes (Sp). Cas9 protein binds to the gRNA first which then complementary binds to the targeted genomic sequences mediating DNA double strand breaks by Cas9. The cell repairs the break using non-homologous end joining which sometimes introduces errors in the form of insertion / deletion (indel) mutations (Doll et al., 2019).

[0074] The genes in pennycress that we mutated to test if seed and plant organ traits could be increased or otherwise agronomically improved were DAI (DA means “large” in Chinese), DA Related (DARI), UBIQUITIN-PROTEIN LIGASE 3 (UPL3), &M DA2. We successfully employed CRISPR-Cas9 and EMS mutagenesis to create numerous different loss-of-function mutations in these genes which, when homozygous, resulted in larger seed size, larger plant sizes, and higher seed oil and / or protein content. Different effects on seed and oil protein content were observed based on which gene or gene combinations were mutated. We have produced many mutant combinations including with tt8 and fael (domestication trait mutations) to create a variety of bigger seed, higher oil, higher protein, lower fiber, and / or lower seed oil erucic acid (C22: 1) phenotypes. TT8 encodes a helix-loop-helix transcription factor which positively regulates proanthocyanidin production in the seed coat of Brassica species and negatively regulates fatty acid biosynthesis (Chen et al., 2014; Nesi et al., 2000). Due to the lack of condensed tannins originating from proanthocyandin production, pennycress tt8 seeds are yellow in color and contain 10% more oil content than wild type (Spring 32) (Esfahanian, 2021 thesis). Wild-type pennycress seeds have high levels of erucic acid (34%) as compared to canola (2%), the latter of which contains knockout mutations in FAE1 (Kim et al., 2015). The FDA has placed limits on erucic acid levels in food and feed due to potential toxicity when eaten in large amounts (Millar and Kunst, 1997). CRISPR-generated fatty acid elongation 1 (fael) mutations created in our lab abolished erucic acid biosynthesis in pennycress seeds making the seed oil edible plus more suitable for industrial uses including conversion to biodiesel, renewable diesel, and sustainable aviation fuel (McGinn et al., 2019).

[0075] UBIQUITIN PROTEIN LIGASE 3 (UPL3)

[0076] A study in Brassica napus and Arabidopsis thaliana (Arabidopsis) (Miller et al., 2019) suggested that a HECT E3 ubiquitin ligase gene named UPL3 mediates ubiquitination and subsequent proteasomal degradation of target proteins including LEC2 which is a transcription factor known to activate the expression of seed maturation and seed lipid accumulation genes (Barthole et al., 2014; Baud et al., 2016). UPL3 overexpression promoted the formation of higher molecular weight forms ofLEC2, likely due to ubiquitylation of LEC2, which correlated with LEC2 degradation (Miller et al., 2019) UPL3 is thought to regulate seed maturation by modulating levels of transcription factors GLABROUS3 (GL3) and ENHANCER OF GLABROUS3 (EGL3), LEC2 during different stages of seed development which in turn modulate expression of other transcription factors and biosynthetic genes involved in testa and embryo development (Patra et al., 2013) (Miller et al., 2019)

[0077] Miller et al. also assessed the potential role of UPL3 in seed formation by characterizing two Arabidopsis T-DNA lines having T-DNA insertions in the 10th exon of UPL3, named respectively SALK 015334 and SALK 151005. The loss-of-function upl3 mutant seeds were found to be 10% larger and contained 12% higher seed lipid content relative to seeds of wildtype Col 0 plants, along with alternations in extrusion of mucilage from the seed coat. Overexpression of N\.UPL3 using 35S Promoter (35S:3HA-UPL3) reduced seed size and seed lipid content (Miller et al., 2019). Furthermore, analysis of seed lipid composition revealed no significant changes in fatty acid composition.

[0078] In another study, the variation in the promoter region and 5’ UTR region of the UPL3 gene in one of the accession of Brassica napus (BnaUPL3.C03) also exhibited larger seeds, lipid content and final crop yield than wild type indicating a potential role of UPL3 in regulating the seed size in Arabidopsis and Brassica napus (Tee, 2019). Reduced BnaUPL3.C03 expression maintains higher levels of LEC2 during seed maturation, prolonging transcriptional activation of storage lipid genes, leading to larger seeds with elevated lipid levels, robust yield increase in field conditions (Miller et al., 2019). Reduced upl3 expression in Brassica napus was also reported to substantially decrease seed glucosinolate content (Miller and Bevan, 2022).

[0079] Apart from its role in seed size, roles for UPL3 in mediating the proteasomal degradation of GL3 and EGL3, both known regulators of trichome morphogenesis have also been studied in Arabidopsis. In Arabidopsis loss-of-function mutation of UPL3 increased leaf hair branching (Downes et al., 2003; El Refy et al., 2004).

[0080] Regarding its expression, in Arabidopsis, UPL3 transcript levels increased steadily during seed development, with highest expression levels observed during the seed maturation phase, suggesting a potential role in seed maturation (Miller et al., 2019).

[0081] Notably, unlike Arabidopsis, pennycress seeds do not extrude mucilage and pennycress plant organs do not have trichomes, highlighting how findings in one plant species cannot predict function in another plant species, even closely related species like Arabidopsis, Brassica napus, and pennycress. Indeed, our studies of pennycress upl3 loss of function mutants did not identify clear differences in seed oil content as was identified in Arabidopsis and Brassica napus but did uncover markedly higher seed protein content (Table 4). Moreover, Miller and Bevan (2022) reported that reduced upl3 expression in Brassica napus resulted in substantially decreased seed glucosinolate content. By contrast, we found that pennycress upl3 mutant seeds do not have reduced glucosinolate content FIG. 10).

[0082] Our findings in pennycress illustrate that one cannot predict how mutations in one plant species will translate to another plant species. Moreover, one cannot predict how changes in seed size will affect seed oil and protein content. As noted above, pennycress upl3 seeds are markedly larger and heavier and have markedly higher protein content but have only marginally higher oil content (FIGs. 4, 6, 7; Table 4). By contrast, pennycress dal darl double mutants (also reported here) produce markedly larger and heavier seeds that have both clearly higher oil and higher protein content (FIGs. 4, 6, 7; Table 4). Also notably, pennycress single mutations in transparent testa 8 (tt8) do not increase seed size but increase seed oil content by 13 percent and seed protein content by over 20 percent (Esfahanian, 2021 thesis). Stacking / bc / knockout with tt8 knockout results in near wild-type amounts of seed oil but that oil lacks erucic acid content.

[0083] DAI (LARGE IN CHINESE 7), DARI (DA 7 -RELATED), and DA 2

[0084] DAI gene (DA means “large in Chinese”) was identified as a novel seed and organ size mutant by a map-based cloning from an EMS-treated M2 population of Col-0 accession (Li et al., 2008). It is known to play a role to set the final seed size and organs in Arabidopsis by controlling the period of cell proliferation in the maternal integuments of developing ovules and seeds. DAI that lies in the ubiquitin-proteasome pathway is a ubiquitin receptor containing two ubiquitin-interacting motif (UIM) domains, a LIM domain, and a C-terminal peptidase domain. UIM domains of DAI have ubiquitin-binding activity in vitro where ubiquitin receptors interact with polyubiquitinated substrates and mediate their degradation (Li et al., 2008). In Arabidopsis, there are seven DA 7-related proteins (named DARI through DAR7) sharing extensive amino acid similarities with DAI, at least two of which (DARI and DAR2) play a role in regulation of endoreduplication in leaf epidermal tissue (Peng et al., 2015). In a study done by Li et al., DNA sequencing on lines generated through EMS revealed that the dal-1 mutants in Arabidopsis have a single-nucleotide G-to-A transition, which is predicted to cause an arginine-to-lysine change in a conserved amino acid at position 385. dal- 1 mutant produced larger size leaves, flowers, seeds, embryos, ovules, seed volume, thick stems as well as increased biomass and normal fertility in Arabidopsis when compared with the wild type Columbia-0 (Li et al., 2008). dal-1 plants went through longer phase of proliferative growth and have a longer life span than wild type plants. The seed mass was increased to 132% of wild type and total yield per plant was increased. It was observed that DAI fimctions by limiting the period of proliferative growth during the early stages of organ development and controls the time taken for most organs to reach their final size and does not affect DAI ubiquitin binding. MDAR1 is the closest homologue of MB Al and interestingly, knock-out of either DA I or its closest homologue DARI displayed no obvious phenotype. Both RxDA l and Rd) ARI have a negative effect on organ size control by restricting the period of cell proliferation in Arabidopsis. The T-DNA insertion mutants dal-ko and darl-1 have not shown apparent phenotypes with respect to seed size. Hence, double dal-koldarl-1 knockout mutants were created which exhibited the original dal-1 phenotype and hence, supported the hypothesis that DAI and DARI act redundantly to restrict seed and organ growth (Li et al., 2008).

[0085] In a study in Brassica napus, BnLM 1 was down-regulated in B. napus by overexpression a functional deficiency of DAI with an arginine-to-lysine mutation at the 358th amino acid The results showed that the biomass and size of the seeds, cotyledons, leaves, flowers and siliques of transgenic plants all increased significantly. In particular, the 1000 seed weight increased 21.23% and the seed yield per plant increased 13.22% in field condition with no negative traits related to yield (Wang et al., 2017)

[0086] In a study in Maize, ZmDA l and ZmDARl were identified as the homologous genes of AADA I and MDAR1. The same single base mutant that leads to an arginine to lysine change at the conserved site was introduced into the ZmDAl and ZmDARl nucleotide sequences (G to A single-base mutant at the 966 bp site in ZmDARl) and called as Zmdal, Zmdarl. Both transgenic events (Zmdal, Zmdarl) showed an increased grain yield by enhancing the grain weight and grain number. In the transgenic plants, more carbohydrate was imported into the kernels through a well-developed endosperm transfer cell layer; this was linked to the increased starch content (Xie et al., 2018). Apart from its role in seed size, a missense mutation in DAI (the dal-1 allele) was found to have fewer lateral branches, which may be due to defects in the initiation of axillary meristems (Li et al., 2020). We did not observe any defects in the pennycress dal mutants we generated. The transcription factors CUC2 and CUC3, which regulate the axillary meristem initiation, directly bind to the / L47 promoter and activate its expression. Hence, mutation in the DAI causes fewer lateral branches due to defects in the initiation of axillary meristems (Li et al., 2020). In another study, overexpressing DA / -like gene of annual wild relative of Soybean Glycine soja (GsoDA i) did not affect seed size but, differentially affected the expression of salttolerance related genes and reduced seed germination sensitivity to salt stress in transgenic Arabidopsis plants (Zhao et al., 2015). Regarding it’s expression, it is highly expressed during the early stages of leaf, petal, integument, and embryo formation and fade away later in petal and leaf development (Li et al., 2008).

[0087] DA2 was identified in Arabidopsis as encoding an E3 ubiquitin ligase that physically interacts with DAI, acting synergistically in controlling cell proliferation in the maternal integument thereby regulating seed size (Xia et al., 2013). Knockout of Arabidopsis DA2 function resulted in larger seeds along with larger cotyledons and flower petals. Overexpression of DA2 in Arabidopsis resulted in smaller seeds and smaller petals. In that study (Xia et al., 2013), the authors did not observe larger seeds or organs in a dal knockout mutant but found markedly larger seeds and organs in dal da2 double mutants and dal darl da2 triple mutants.

[0088] Wild type pennycress dal, darl, upl3 and da2 nucleic acid sequences are shown in SEQ ID NOs: 1, 3, 5, and 7, respectively, and wild type pennycress DAI, DARI, UPL3 and DA2 polypeptide sequences are shown in SEQ ID NOs: 2, 4, 6, and 8, respectively.

[0089] Also provided are nucleic acids and polypeptides that differ from SEQ ID NOs: 1, 3, 5, and 7 and SEQ ID NOs: 2, 4, 6, and 8, respectively. Nucleic acids and polypeptides that differ in sequence from SEQ ID NOs: 1, 3, 5, and 7 and SEQ ID NOs: 2, 4, 6, and 8, can have at least 80% sequence identity (e.g., at least 85%, 90%, 95%, 96%, 97%, 98%, or 99% sequence identity) to SEQ ID NOs: 1, 3, 5, and 7 and SEQ ID NOs: 2, 4, 6, and 8, respectively.

[0090] In calculating percent sequence identity, two sequences are aligned and the number of identical matches of nucleotides or amino acid residues between the two sequences is determined. The number of identical matches is divided by the length of the aligned region (i.e., the number of aligned nucleotides or amino acid residues) and multiplied by 100 to arrive at a percent sequence identity value. It will be appreciated that the length of the aligned region can be a portion of one or both sequences up to the full-length size of the shortest sequence. It also will be appreciated that a single sequence can align with more than one other sequence and hence, can have different percent sequence identity values over each aligned region.

[0091] The alignment of two or more sequences to determine percent sequence identity can be performed using the algorithm described by Altschul et al. (1997, Nucleic Acids Res., 25:3389 3402) as incorporated into BLAST (Basic Local Alignment Search Tool) programs, available at ncbi.nlm.nih.gov on the World Wide Web. BLASTN is the program used to align and compare the identity between nucleic acid sequences, while BLASTP is the program used to align and compare the identity between amino acid sequences. When utilizing BLAST programs to calculate the percent identity between a sequence and another sequence, the default parameters of the respective programs generally are used.

[0092] A skilled artisan will appreciate that a loss-of-fimction mutation can be introduced into a wild type nucleic acid (i.e., SEQ ID NOs: 1, 3, 5, and / or 7) as well as sequences having at least 80% sequence identity (e.g., at least 85%, 90%, 95%, 96%, 97%, 98%, or 99% sequence identity) to SEQ ID NOs: 1, 3, 5, and / or 7, thereby leading to changes in the amino acid sequence of the encoded polypeptide. For example, changes can be introduced into nucleic acid coding sequences using CRISPR / Cas technologies, mutagenesis (e.g., site-directed mutagenesis, PCR-mediated mutagenesis), or chemical synthesis. Such loss-of-fimction mutations can lead to, for example, reduced or absent transcription and / or translation, disrupted functionality of the resulting polypeptide, truncation of the resulting polypeptide, etc. Loss-of-fimction does not require 100% absence of functional polypeptide; as used herein, loss-of-fimction refers to a reduction in the amount of functional polypeptide present in the plant generally or at a specific developmental stage such that the desired phenotype is observed (e.g., increased plant size, increased seed size, increased seed weight, increased seed oil content, and / or increased seed protein content).

[0093] A number of representative single, double, triple, quadruple, and quintuple mutations in various combinations of dal, darl, upl3, and da2 were generated using various backgrounds. The mutant nucleic acid (odd) and polypeptide (even) sequences are shown in SEQ ID NOs: 9- 136. See Table 2 and Example 22. Mutant plants as described herein exhibited increased plant size, increased seed size, increased seed weight, increased seed oil content, and / or increased seed protein content at statistically significant levels relative to plants lacking the corresponding mutations. As used herein, statistical significance refers to a p- value of less than 0.05, e.g., a p- value of less than 0.025 or a p-value of less than 0.01, using an appropriate measure of statistical significance, e.g., a one-tailed two sample t-test.

[0094] In accordance with the present invention, there may be employed molecular biology, microbiology, biochemical, and recombinant DNA techniques within the skill of the art. Such techniques are explained fully in the literature. The invention will be further described in the following examples, which do not limit the scope of the methods and compositions of matter described in the claims.

[0095] EXAMPLES

[0096] Example 1 — CRISPR Design

[0097] To identify the putative TaDAl, TaDARl, TaUPL3, and DA2 gene orthologues in pennycress, Arabidopsis thaliana coding sequences (CDS)- / )4 / (AT I Gl 9270). DARI (AT4G36860), DA2 (AT1G78420), and UPL3 (AT4G38600) were obtained from the website The Arabidopsis Information Resource (TAIR). Using the obtained Arabidopsis CDS as query for each gene, a search was made on the National Centre for Biotechnology Information (NCBI) with a nucleotide BLAST tool under Thalspi arvense transcriptome shotgun assembly and (TSA:Dorn, 2015) GAKE 01008777.1 (TciDAPp GAKE 01001336.1 (TaDARl), GAKE 01008260.1 (TaDA2), and GAKE 01002387.1 (Ta / / / 7.3) sequences were obtained. After identifying the Open Reading Frame (ORF), whole genomic sequence of each gene plus their upstream and downstream sequences were predicted by blasting their ORF at specific Basic Local Alignment Search Tool (BLAST) program freely available at pennycress.umn.edu.

[0098] To increase the efficiency of genome editing and having higher mutation rate in our gene of interest, we designed CRISPR / SpCas9 binary vector constructs containing either 4 guide RNAs or 2 guide RNAs within each gene driven by U6-26 and U6-29 promoters using cloning vectors pCBC_ / l / 7 / 2 (for 2 gRNA) and pCBC_ / 9 / 7 T2 and pCBC BJMOD (for 4gRNA) and a binary vector pEC666Red3 (DsRED). To create a double knock-out of daJ darl, 4 guide RNAs that targets both DAI and DARI gene were designed as shown in FIG. 1. These constructs utilized a vector set containing Streptococcus pyogenes Cas9 (Spcas9) which was used in Maize by Wang et al., 2015. PAM site for Spcas9 is 5 '-NGG-3'. pHEE401E was a gift from Qi-Jun Chen (Addgene plasmid # 71287; http: / / n2t.nct / addgene:71287 ; RRID:Addgene_71287. The plant selectable hygromycin marker, Hygromycin phosphotransferase gene (hpt) was swapped out of the pHEE401E plasmid and replaced with a DsRED plant selectable marker by one of former PhD student in the lab and hence named as pEC666Red3 (Jarvis, thesis). Bacterial selection used for the binary vector (pEC666Red3) was 50 pg / ml kanamycin whereas, NIGHTSEA flashlight (model DFP- CG) at a wavelength of 510-540 nm (emission 600nm) was used for plant (transformants) selection.

[0099] Example 2 — Agrobacterium-Mediated Transformation of Pennycress

[0100] The multi-guide RNA construct was cloned into Agrobacterium tumefaciens. Table 1 depicts the protospacers used in these constructs. Cultures of Agrobacterium strain GV3101 containing these constructs were grown from the glycerol stocks (-200 pl inoculated into 50 ml Luria Broth (LB) containing 50 pg / ml gentamicin, 50 pg / ml rifampicin and 50 pg / ml kanamycin into 50 ml culture. The 50 ml cultures were shaken overnight at 28°C, then it was added to 400 ml LB antibiotics-containing media and incubated one more night. The following day it was centrifuged at 4500 RPM for 10 mins and resuspended in dipping solution containing 5% sucorose (w / v) and 0.02% (v / v) Silwet-77. The flowers of the plant opened for 5-6 days were submereged in the Agrobacterim containing dipping solution, then placed under -30 inches mercury (14.7 psi) vacuum in a 26 cm x 25 cm x 36.5 cm vacuum chamber for 10 mins using a diaphragm vacuum pump (60 L / Min pump speed, 200 mBar ultimate vacuum. Immediately after dipping, the floral portions were individually wrapped with a plastic wrap and the plants were placed back into the in environment-controlled growth chambers cycling 16 hours light / 8 hours dark (175-250 pE m2s1light intensity) at 21°C. The plastic wraps were removed the following day (McGinn et al., 2019). Each of the CRISPR constructs designed were dipped in plants with spring background named as “Spring 32” having black seeds, as well as pennycress that has knock out mutations in TRANSPARENT TESTA 8 (TT8) with an insertion of base A, and FATTY ACID ELONGATION 1 (FAEI) gene which has a deletion of 4 bp in Spring32 background named as “tt8fael ” with yellow-colored seeds. Table 1 — Protospacers used to generate CRISPR-Cas9 pennycress mutants

[0101] Example 3 — Surface Sterilization of Pennycress Seeds and Growth Conditions The dipped plants with Agrobacterium containing the desired guide RNAs were grown, and the seeds were harvested once the plants were senesced. The seeds were surface sterilized with brief rinse of 95% ethanol followed by 20 min incubation in the sterilizing solution (50%bleach, 0.01%SDS). For seeds having light color and thin coat (tt8fael seeds) sterilizing solution consisting of 30% bleach and 0.01% SDS was used. After the removal of sterilizing solution, the seeds were rinsed 3 times with sterile distilled water. The surface sterilized seeds were plated on agar / one half-strength MS (Murashige and Skoog) media in petri dishes wrapped with a parafilm. The plates were placed into a Percival Scientific CU-36L5 incubator (16 hours 4100K fluorescent -150-200 pE / m2 / s / 8 hours dark, 22°C).

[0102] Example 4 — Screening and Selection of Transgenic Plants under DsRed and Hygromycin

[0103] After a week or 10 days, the seeds on the plate were observed under the fluorescence screening using a Nightsea dual fluorescent protein flashlight. Since the binary vector contains DsRed as a plant selectable marker, only the transformants fluorescence red. Those seedlings were transplanted at a density of 4 plants per 4-inch pot in autoclaved Miracle Grow soil mix intermixed with 0.03g / 4 inch of the insecticide Marathon (Marathon 1% granular form) and grown in environment-controlled growth chambers (175-250 pE m2s ' light intensity cycling 16 hours light / 8 hours dark at 21 °C).

[0104] For selection of transformants carrying the hygromycin resistance selectable marker HPT, seeds were plated on 0.8% agar / one half-strength MS (Murashige and Skoog) media with 40 pg / ml hygromycin (HYG+) to screen for putative transformants. Seeds were also plated onto media without hygromycin (HYG-) as controls. Since the binary vector contains the HPT gene conferring hygromycin resistance as a plant selectable marker, only transgenic seedlings with the inserted CRISPR-Cas9 and guide RNA constructs can survive in media with hygromycin whereas the non-transformants die due to hygromycin toxicity. After 10-12 days, the transformants were then transplanted into the soil to grow for further experiments.

[0105] Example 5 — Performing PCR and Sequencing to Confirm the Mutations

[0106] As the transformants were grown, leaf tissue of each putative mutant was harvested and ground using a Qiagen tissuelyser. Genomic DNA was extracted using a modified CTAB protocol routinely used in the lab followed by PCR amplification using the Taq DNA polymerase and gene specific primers flanking our CRISPR target sites PCR thermal cycles were as follow- 1 min initial denaturation at 98°C for 1 min, 33 cycles of 98°C for 10 sec, annealing at 56°C for 15 secs, and a 72°C extension for 1.30 mins followed by single 5 min final extension at 72°C. The PCR products were electrophoresed in 0.8% agarose gel and were purified using the GeneJet Gel Extraction Kit (#KO692). The purified PCR products were sent to Eurofins Genomics for DNA sequencing. Lazergene software tools was used to identify the CRISPR / Cas9 induced mutations like insertions, deletions, or inversion of nucleotides. The identified mutants were moved forward and genotypically and phenotypically analyzed in the subsequent generations (described next).

[0107] Example 6 — Selection of Plants Without Cas9 and Analyses in Growth Chambers

[0108] The Ti plants did not seem to have off-target mutations and hence, the transformants (Ti) were moved forward to second generation (T2). The CRISPR / Cas9 machinery typically inserts in a chromosomal location that can be segregated away from the induced mutations, based on traditional Mendelian inheritance in the T2 generation, with 75% plants containing the CRISPR / Cas9 transgenes (Cas9) and 25% plants being Cas9 free. In the second generation, as expected Cas9 machinery underwent the segregation. Genomic DNA was extracted from the leaf tissues and PCR was performed with Cas9 primers and gene specific primers. The purified PCR products were sequenced to confirm the mutations and hence, mutants without Cas9 were moved forward for the detailed analyses in the third generation (T3).

[0109] Finally, homozygous and Cas free lines for different mutants were obtained.

[0110] Example 7 — Characterization Assays

[0111] Phenotypical assessments were performed on mutants which were CRISPR-Cas9 construct-free along with corresponding controls. Statistical analysis were determined using either a Student’s T-Test or one-way ANOVA and Tukey’s test or a Sidak test.

[0112] Example 8 — Seed Size Analyses

[0113] Quantification of number of seeds, seed size, total seed weight per plant, thousand grain weight (TGW) per plant of different WT and CRISPR-mutants were performed using a MARViTECH MARViN 5.0 seed analyzer instrument along with an Ohaus STX223 balance. The device is calibrated for pennycress seeds that measures length and width of each individual seed. Up to 1000 seeds could be spread onto the measuring surface where the digital built-in camera records the images and image processing software will analyze those images. Seeds were collectively weighed, and the results were obtained in a log table transferable to Excel. Based on the coded cues provided in the control images, chaff and other debris could be removed prior to being weighed, serving to provide an accurate analysis of both wild type and mutant lines. Statistical analyses were performed using ANOVA to determine whether seed size increased significantly.

[0114] Example 9 — Seed Glucosinolate Analyses

[0115] Seed glucosinolate predictions were made using a Metrohm NIRS XDS MultiVial Analyzer as described in Chopra et al., 2019. In short, Near Infrared Spectral (NIRS) scans of 75 to 500 seeds in 15 x 19 mm clear glass vials were collected and analyzed using calibration curves based on those developed for canola.

[0116] Example 10 — Plant Heights

[0117] Final height of each plant was recorded by measuring the length from the base of the pot to the top of the main stem straightening stem to against a measuring ruler.

[0118] Example 11 — Determination of Total Seed Oil Content

[0119] Total oil and moisture content were determined by non-destructive method TD-NM on 450 mg of samples of whole pennycress seeds, using a Bruker Minispec MQ40 with a 39.95 MHZ NMR frequency and 40°C magnet temperature. A percent oil calibration curve was constructed within the Bruker version 7.0.0 software by measuring the signal for six oil standards between 20% -40% oil concentration based on a 450 mg sample.

[0120] To determine percent oil and moisture in seeds from the sample plots, an analytical balance was used to weigh 450 mg of seed from each plot into 10 mm flat bottom NMR tubes. After tempering the samples at 40°C for 30 minutes, they were scanned in the MQ40 and the percent oil and moisture were calculated using our calibration curves and the Bruker Oil and Moisture in Seeds application in minispec Plus software. The results from minispec Plus were exported to an Excel file. Using Excel, the percent oil on a dry weight basis was calculated by dividing the milligrams of oil in each sample by the dry weight of each sample and multiplying by 100.

[0121] Seed lipid fraction quantification was performed as described in Zhai et al., 2023.

[0122] Example 12 — Determination of Protein Content

[0123] Protein content was determined as described in Zhai et al., 2023. Example 13 — CRISPR Results

[0124] We have successfully created CRISPR / Cas9 binary vectors containing either two or four different protospacers (FIG. 1; Table 1). TaUPL3, TaDA / , TaDARl , and TaDA2 are predicted to have open reading frames (ORFs) of 5676 bp, 1599 bp, 1662 bp, and 1236 bp in length, with nucleotide sequence identities of 91.1%, 87.8%, 90.5%, and 88% to UPL3 (AT4G38600), DAI (AT1G19270) and DARI (AT4G36860), and DA2 (AT1G78420) in Arabidopsis thaliana, respectively.

[0125] Three independent homozygous mutations in UPL3 V / QXQ obtained (Table 2) targeting each single gene. These mutations also were stacked with “ / / S fuel" by dipping the constructs in tt8 fael mutants that has mutations as +A in TT8 gene and -4bp in FAE1 gene to obtain different mutant combinations.

[0126] Table 2 — Pennycress CRISPR-Cas9-Induced and EMS-Induced Mutations

[0127] Table 3. Pennycress CRISPR-Cas9-induced mutations in various natural accessions and tt8fael domesticated line

[0128] Example 14 — Phenotypic Changes Observed in upl3 and upl3 tt8 fael Mutants in Pennycress To determine if any of the mutations have pleiotropic effects on plant growth and seed viability, homozygous mutants with their appropriate controls were grown side by side in growth chambers and were assessed for relative growth characteristics including final plant heights, pod size and seed size measurements for all of the genotypes. All three mutants upl3-l, upl3-2, and upl3-3 plants grew to be significantly taller by around 28-30% (FIG. 3D), have bigger seeds and pods (FIGs. 2 and 3). upl3 mutants stacked with (upl3tt8fael)-l, (upl3tt8fael)-2, and (upl3tt8fael)-3 in pennycress also produced taller plants, bigger flowers, bigger pods, and bigger seeds suggesting a role of upl3 in restricting seed and organ size (FIGs. 2 and 5).

[0129] Example 15 — UPL3 Gene Mutations Increase Seed Size and Seed Weight in Pennycress In a study done by Miller et al., loss-of function mutants of UPL3 in Arabidopsis increased seed size by nearly 10% (Miller et al., 2019). In our study in pennycress, five biological replicates were analyzed for statistical differences between the various mutants and wild-type lines grown in chamber conditions. Wild-type (Spring32) seeds were found to have an average area of 1.9 mm2. While in the three mutants upl3-l, upl3-2, and upl3-3 mutants, the average seed area was 2.5 mm2, 2.6 mm2, 2.3 mm2i.e., increased by 30%, 31%, and 17% respectively (FIG. 3A). The thousand grain weight for all the mutants was higher as compared to wild type suggesting they were heavier in weight by 20-51% (FIG. 3B). 15 pods were taken from each plant and the seeds in each pod were counted (FIG. 3C). There was a range of average numbers of seeds per pods of mutants which were not significantly different from wild type.

[0130] Example 16 — Stacking of npl3 with tt8 fael Increases Seed Size and Weight in the Domesticated Lines of Penny cress

[0131] We were able to characterize penny cress up! 3 mutants as individual gene changes as well as further in triple stacks in the domesticated background (tt8fael). Five biological replicates were analyzed for statistical differences between the various mutants and wild-type lines in chamber conditions. (upl3tt8fael)-l (~326bp, -4bp). (upl3tt8fael)-2 (-326bp, -4bp\ and (upl3tt8fael)-3 (-22 bp) are three independent homozygous mutants whose seed area on average has increased by 28%, 25%, 38% as compared to their controls in growth chamber conditions (FIG. 5A). Thousand Grain Weight (TGW) for the mutants increased by 19-39% (FIG. 5B). Similar to upl3 mutants, total number of seeds per pod in upl3tt8fael mutants were comparable to the tt8fael control (FIG. 5C). FIG. 5D quantitatively represents that all three upl3tt8fael mutants plants grew to be significantly taller than control tt8fael, on average 73 to 75 cm, respectively versus 62 cm for control, representing around 23% taller heights in chamber conditions. Comparable results were obtained in the 1t8 fael domestication traits background (FIGs. 4, 6 and 13).

[0132] Example 17 — Pennycress UPL3 Loss-of-Function Mutations Decrease or Do Not Change Oil Content and Alter Seed Oil Fatty Acid Composition

[0133] Pennycress seeds contain -34% oil by weight in the form of Triacylglycerols (TAGs) which serve as an energy source for the seed embryo upon germination, before the young seedling reaches sunlight and can photosynthesize (Boateng et al., 2010; Moser et al., 2009). The oil composition of pennycress is made mostly of C22: l erucic acid (32.8%), Cl 8:2 linoleic acid (22.4%), and C18: 1 oleic acid (11%) (Moser et al., 2009).

[0134] In Arabidopsis, the loss-of- function of UPL3 resulted in 12% higher seed lipid content relative to seeds of wild-type Col 0 plants whereas, overexpression of MIJPL3 using 35S Promoter (35S:3HA-UPL3) reduced lipid content (Miller et al., 2019). Furthermore, analysis of seed lipid composition revealed no significant changes in fatty acid composition. Similarly, in another study, the variation in the promoter region of the UPL3 gene in one of the accessions of Brassica rapa also exhibited higher lipid content. However, UPL3 loss of function mutations in pennycress either did not increase seed oil content or slightly decreased seed oil content (FIG. 7) These results were obtained using two different seed oil quantification methods, and similar results were observed with upl3 mutations in the tt8 fael domestication traits background (FIGs. 7, 8 and 14). Also, different from Arabidopsis upl3, we found that pennycress upl3 mutant seed oil fatty acid content had significantly more oleic acid and less erucic acid comparted to wild type (FIG. 9).

[0135] Example 18 — Pennycress UPL3 Loss-of-Function Mutations Increase Seed Protein Content Quantification of seed protein content revealed that pennycress upl3 mutant seeds had more total protein compared to wild type and ttS fael controls (Table 4), which may explain in part the heavier upl3 mutants’ seed weight (FIG. 6).

[0136] Example 19 — Pennycress UPL3 Loss-of-Function Mutations Do Not Decrease Seed Glucosinolate Content

[0137] Reduced upl3 expression in Brassica napiis was reported to substantially decrease seed glucosinolate content (Miller and Bevan, 2022). Therefore, we determined pennycress upl3 mutant seed glucosinolate content, finding no or little differences from wild type (FIG. 10).

[0138] Table 4 — Fractionation of dry seed biomass-protein data

[0139] Example 20 — Plant Organ Sizes and Seed Size and Weight Increases Observed in dal, darl, dal darl Mutants in Penny cress

[0140] As seen in FIGs. 10 and 11, double knock out of DAI and DARI resulted in increased plant height in pennycress, daldarl-1, daldarl-2, daldarl-3 mutants grew to be significantly taller than wild type (Spring 32) respectively, on average 55 cm, versus 64-67 cm for wild type, representing around 17-20% taller heights (FIG. 1 ID). All three dal darl mutants also had larger leaves, flowers, seeds, and pods as compared to wild type (FIG. 10). These increases were also consistently observed for dal darl mutations under different growth conditions and in the tt8 fael domestication traits background (FIGs. 4, 6, 12, and not shown). In Arabidopsis, dal-kol darl-1 double mutant produced larger flowers than the wild type which is similar to our observations in pennycress (Li et al., 2008; Xia et al., 2013).

[0141] In a study done by Li et al., knock-out of either DA I or its closest homologue DARI displayed no obvious phenotype. However, dal-kol darl-1 double mutants had significantly increased seed size in Arabidopsis. In our study, the single knock-out mutant of DAI has also increased seed size in penny cress as seen in FIG. 11 A. dal-1, dal-2, da 1-3 are three independent homozygous mutants whose seed area has increased by 16%, 20 % and 14 % as compared to WT respectively, darl-1, darl-2, darl-3 represents three independent homozygous mutations in DARI gene whose seed area does not seem to be increased much and is somewhat similar to WT. Interestingly, dal darl double mutants (fdaldarl)-l, (daldarl)-2, (daldarl)-3) had significantly increased seed size by 39 to 40% in pennycress. Thousand Grain Weight (TGW) has increased by 12-19% in dal mutants whereas, in daldarl double mutants it has increased by 38-42% as compared to WT (FIG. 1 IB). The number of seeds per pod is not much variable than seen in the wild type plants (FIG. 11C). Comparable results were obtained with under different growth conditions (FIGs. 4 and 6) and in tt8 fael domestication trait backgrounds (FIGs. 4, 6 and 13).

[0142] Example 21 — Seed Oil and Protein Content Increases Observed in dal, darl, dal darl Mutants in Pennycress but No Changes Observed in Seed Oil Fatty Acid Composition Quantification of seed oil and protein content revealed that pennycress dal darl mutant seeds had substantially more total oil and protein compared to wild type and tl8 fael controls (FIG. 7 and Table 4). These results differed from those of the upl3 mutants in that upl3 mutant seeds only exhibited higher protein but not higher oil content (FIG. 7 and Table 4). Moreover, dal darl mutant seed oil fatty acid content did not differ from wild type in contrast to that of upl3 (FIG. 9).

[0143] Example 22 — Sequences

[0144] The double underlining delineates locations of protospacers (PS) used for CRISPR- Cas9 gene editing.

[0145] DAI Wild-Type Pennycress Open Reading Frame ( ORF) ( SEQ ID NO : 1 ) :

[0146] ATGGGTTGGTTCAACAAGATCTTCAAAGGCTCTAACCAAAGGCTCCGGGTGGGGAATGAGCACAAT CACAATGGCTATTACGAGAACTATCCAAATGCGCATGCTTCACACGACGAGCCTAGTGCGGATACA GATGCTGATCGTGATGAACCTCAGACGCAGGAATCATCTACATCTGAGGATACGTCGAATGACCAG GAAAATGAAGACATAGACCGTGCAATCGCGTTGTCTCTTTTAGAAGAGAATCAAGGACAGAAAAAT ACAGGCGGGAAATACGTAATGGTGGATGAAGATGAGCAGCTTGCTAGAGCCATACAAGAAAGTATG ATAGTTGGGAATTCACCGCGTCACAAAAGTGGAAGTACGTATGATCATGGGAATGCATATGGGACT GCAGATTTATTTGGGAATGGTCACATGCATGGAGGAGGAAATGTATATGCAAATGGAGATATTTAT TACCCAAGACCCGTTGCTTTTTCAATGGATTTCAGGATTTGTGCTGGCTGCAATATGGAGATTGGC CATGGAAGATTTCTGAATTGCCTTAACTCACTATGGCATCCAGAATGTTTTCGATGTTATGGCTGT AGTCACCCGATTTCTGAGTACGAGTTTTCAACATCTGGGAACTACCCTTTTCACAAGGCTTGTTAC AGGGAGAGATATCATCCAAAATGTGATGTCTGCAACCACTTTATTCCAACAAATCCTGCTGGTCTT ATTGAATATAGGGCACACCCATTTTGGATCCAGAAGTATTGCCCTTCTCACGAACACGATTCCACC CCAAGATGTTGCAGTTGTGAAAGAATGGAGCCACGGAATACGGGATATATTGAGCTTAATGATGGA CGTAAACTTTGCCTCGAGTGTCTGGACTCAGCGGTCATGGACACAAGTCAATGTCAACCTTTGTAC TTGCAGATACAAGATTTCTATGAAGGGCTTAACATGAAGGTAGAGCAGGAAGTTCCACTGCTCTTG GTTGAGAGGCAAGCACTTAACGAAGCCAGAGAAGGTGAAAAGAATGGTCACTATCACATGCCAGAG ACAAGAGGACTGTGTCTTTCAGAAGAACAAACTGTTAGTACTATAAGAAAGCGATCAAAGGGAAAT TGGGGTGGGAATATGATCACAGAGCCGTACAAGTTAACTCGTCAATGCGAAGTTACTGCCATTCTC ATCTTATTTGGGCTCCCTAGGCTACTGACTGGTTCGATTCTAGCTCACGAGATGATGCACGCGTGG ATGCGGCTGAAAGGATTCCGGACACTGAGCCAAGACATTGAAGAAGGTATATGTCAAGTGATGGCT CATAAGTGGATAGAAGCCGAGTTAGCTGCTGGTTCAAGAAACAGCAGTGCTGCATCATCATCATCA TCGTCATCATCTTCTTCTTCTACTAGAGGACTGAAGAAGGGACCAAAATCGCAGTACGAGAGGAAG CTTGGCGAGTTTTTCAAGCACCAGATCGAGTCTGATGCGTCTCCGGTTTATGGAGACGGGTTCAGG GCAGGGCGGCTAGCGGTTAACAAGTATGGTTTGCGAAAAACGCTTGAGCATATACAGATGACCGGT AGATTCCCGGTTTAA

[0147] DAI Wild-Type Pennycress protein sequence ( SEQ ID NO : 2 ) :

[0148] MGWFNKIFKGSNQRLRVGNEHNHNGYYENYPNAHASHDEPSADTDADRDEPQTQESSTSEDTSNDQ

[0149] ENEDIDRAIALSLLEENQGQKNTGGKYVMVDEDEQLARAIQESMIVGNSPRHKSGSTYDHGNAYGT ADLFGNGHMHGGGNVYANGDIYYPRPVAFSMDFRICAGCNMEIGHGRFLNCLNSLWHPECFRCYGC SHPISEYEFSTSGNYPFHKACYRERYHPKCDVCNHFIPTNPAGLIEYRAHPFWIQKYCPSHEHDST PRCCSCERMEPRNTGYIELNDGRKLCLECLDSAVMDTSQCQPLYLQIQDFYEGLNMKVEQEVPLLL

[0150] VERQALNEAREGEKNGHYHMPETRGLCLSEEQTVSTIRKRSKGNWGGNMITEPYKLTRQCEVTAIL ILFGLPRLLTGSILAHEMMHAWMRLKGFRTLSQDIEEGICQVMAHKWIEAELAAGSRNSSAASSSS SSSSSSSTRGLKKGPKSQYERKLGEFFKHQIESDASPVYGDGFRAGRLAVNKYGLRKTLEHIQMTG RFPV . DARI Wild-Type Pennycress ORF ( SEQ ID NO : 3 ) (part of PS5 lies in intron ( TAA at the end ) ) :

[0151] ATGGGGTGGCTTACTAAAATCCTTAAAGGCTCAAGCCATAAATTCTCGGATGGACAAGTTAACAGA

[0152] AGATATAGAGATGATAGGAACTTGGAAAGTTCTCGTCTTTCTGCGGATGGATCTGATTTTGACAAA

[0153] GAAGAGATTGAATGTGCTATTGCACTCTCTCTTTCAGAACAAGAACATGTAATTCCACAAGATGAC

[0154] AAAGGAAAACAAGTAATCGAATACAAATCTGAAACCGAAGAAGATGAGGATGAGGATGAGGACGAA

[0155] GATGAAGAATACACGAGAGCTCAGCTAGAAGCAGCGGAAGAAGAGGAAAGACGGGCAGCTCAAGCT

[0156] CAAATCGAGGAAGAAGAGAAACGGCGAGCTGAAGCTGAATTAGAAGAAATAGAGAAACAGCTTGCG

[0157] AAAGCTCGACTAGAAGAGGAAGAAGTTAGGCGTACCAAAGCGCAATTGGAGGAAGACGAGCAACTA

[0158] GCTAAGGCCATTCAAGAAAGTATGGTTGTGGGATCTCCTCCTCGGTATGATTCTGGAAGTATGTTT

[0159] CAACCATTCCCTTTCCTTAATCCATCTAGTCACAGGGGTTGCCTAGCTGAGATTGGACATGGAAGG

[0160] TTTCTGAGTTGCATGGGTGGCGTTTGGCATCCTGAATGTTTCTGCTGCCATGCTTGTGATAAGCCC

[0161] ATCATAGATTATGAGTTCTCAATGTCCGGGAATCGGCCTTATCACAAGCTATGTTACAAGGAGCAG

[0162] CATCATCCTAAATGTGATGTTTGCCATAACTTTATTCCGACGAATTCAGCTGGTCTCATTGAGTAC

[0163] AGGGCACATCCCTTTTGGATGCAAAAGTATTGTCCTTCACATGAGCGTGATGGAACTCCTCGTTGC

[0164] TGTAGCTGTGAGCGAATGGAGCCAAAAGATACAAAATATCTCATTCTTGATGATGGTCGAAAACTG

[0165] TGTCTTGAATGTCTCGACTCAGCCATTATGGACACTCACGAATGCCAACCATTGTACCTTGAGATA

[0166] CGTGAGTTTTATGAAGGCTTACACATGAAAGTAGAACAGCAGATTCCTATGCTCTTGGTGGAGAGA

[0167] TCAGCTTTAAACGAAGCTATGGAAGGAGAGAAACACGGACATCATCATTTACCTGAAACCAGAGGA

[0168] CTCTGTTTGTCTGAGGAACAAACTGTCACAACGGTGTTAAGGAGACCAAGAATTGGGGCAGGCTAC

[0169] AAATTGATAGACATGATCACAGAGCCTTGCAGGCTGATACGTCGTTGTGAAGTCACTGCAATTCTC

[0170] ATTCTATATGGACTTCCTCGTTTGTTAACTGGATCAATCCTTGCTCATGAGATGATGCATGCCTGG

[0171] CTTCGACTAAATGGGTATCCGAATCTTAGGCCAGAAGTTGAAGAAGGAATATGTCAGGTTTTGGCT

[0172] CACATGTGGTTGGAATCTGAGACTTATGCTGGCTCTACATTAGTAGATATCGCATCTTCTTCATCA

[0173] TCATCATCATCGGCTGTGGTCACTGCATCGTCAAAGAAAGGTGAGAGGTCTGACTTTGAGAAGAAA

[0174] CTTGGTGAGTTTTTCAAGCACCAAATAGAGTCGGATTCTTCGTCGGCATATGGGGATGGGTTCAGG

[0175] CAAGGTAACCAAGCAGTTCTTACGCACGGTCTGAGGCGAACCCTCGACCATATTCGCTTGACCGGT ACATTTCCTTAA

[0176] DARI Wild-Type Pennycress protein sequence ( SEQ ID NO : 4 ) :

[0177] MGWLTKILKGSSHKFSDGQVNRRYRDDRNLESSRLSADGSDFDKEEIECAIALSLSEQEHVIPQDD

[0178] KGKQVIEYKSETEEDEDEDEDEDEEYTRAQLEAAEEEERRAAQAQIEEEEKRRAEAELEEIEKQLA

[0179] KARLEEEEVRRTKAQLEEDEQLAKAIQESMVVGSPPRYDSGSMFQPFPFLNPSSHRGCLAEIGHGR

[0180] FLSCMGGVWHPECFCCHACDKPIIDYEFSMSGNRPYHKLCYKEQHHPKCDVCHNFIPTNSAGLIEY

[0181] RAHPFWMQKYCPSHERDGTPRCCSCERMEPKDTKYLILDDGRKLCLECLDSAIMDTHECQPLYLEI

[0182] REFYEGLHMKVEQQIPMLLVERSALNEAMEGEKHGHHHLPETRGLCLSEEQTVTTVLRRPRIGAGY

[0183] KLIDMITEPCRLIRRCEVTAILILYGLPRLLTGSILAHEMMHAWLRLNGYPNLRPEVEEGICQVLA

[0184] HMWLESETYAGSTLVDIASSSSSSSSAVVTASSKKGERSDFEKKLGEFFKHQIESDSSSAYGDGFR QGNQAVLTHGLRRTLDHIRLTGTFP .

[0185] UPL3 Wild-Type Pennycress ORF ( SEQ ID NO : 5 ) :

[0186] ATGGAAACTCGGAGCCGCAAGCGGGCGGAGGCGACCTCAACTGCCCCATCTTCTTCTTCTTCTTCA

[0187] CCTCCTCCTCCCTCAGGCCCCACCACCCGCAGCAAACGCGCTCGTCTTTCCTCTTCCTCTTCCTCC

[0188] TCTGTTGCCGCTACCACCACTGCACCTTCCTCTTCCACCCGCTCTCGCTCTTCTCGCTCCGCCGCC

[0189] GCCGCTGCTACTCCCATGGACACCTCCACCGAGTCTTCTGGATTTCGCCGAGGCGGACGAGGTAAC

[0190] AGGGGTAACGACAACAGTAATTCTGACAAGGGCAAGGAGAAGGAGCACGACGTTAGGATTAGGGAT

[0191] AGAGAAAGAGAAAGAGACCGAGCCAGAGAACAACTCAACATGGATGCTGCCGCTGCTGCTGCCGCC

[0192] GCTGACGACGACGACGACAATGACAGCGAGGATGGCAACGGGGGTTTCATGCATCCCAACATGAGC

[0193] TCCGCCAGCAGTGCTTTACAAGGTTTGCTGAGGAAGCTTGGAGCTGGCCTCGATGACTTGCTTCCT

[0194] TCCTCTGGTATCGGCTCTGCTTCTTCTTCCCACTTAAATGGGAGGATGAAGAAGATACTCTCTGGC

[0195] TTGCGCTCCGAAGGAGAAGAGGGAAAGCAGGTCGAGGCTTTAACCCAGCTATGCGAGATGCTATCC ATTGGGACTGAAGACTCATTAAGCACCTTCTCTGTTGATTCCTTCGTCCCTGTTCTCGTTGGTCTA

[0196] CTAAACCATGAAAGCAATCCCGACATAATGCTTCTTGCGGCCAGGGCTCTTACCCATCTATGTGAT

[0197] GTTTTGCCGTCTTCTTGTGCCGCCGTTGTACATTACGGCGCTGTTTCATGCTTTGTCGCCAGATTG

[0198] CTAACCATCGAGTACATGGACTTGGCCGAGCAGTCTCTGCAAGCTCTCAAAAAGATATCTCAGGAG

[0199] CACCCAACTGCCTGTTTGCGAGCTGGCGCTCTGATGGCAGTGCTCTCATATCTTGATTTCTTCTCC

[0200] ACTGGAGTCCAGCGTGTAGCACTATCTACTGCTGCAAATATGTGCAAGAAATTACCTTCTGATGCA

[0201] TCTGATTATGTTATGGAAGCTGTACCATTACTGACAAACCTACTTCAGTATCATGATTCGAAGGTT

[0202] TTGGAATATGCTTCTATATGTCTGACTCGGATTGCTGAAGCATTTGCTTCGCACCCTGAGAAATTG

[0203] GATGAATTATGCAACCATGGCCTTGTGACTCAAGCTGCGTCTCTTATTTCCACTACCAACTCAGGA

[0204] GGTGGGCAAGCATCTCTGGGCGTTTCAACATACACGGGGTTAATCCGATTACTTTCCACCTGTGCG

[0205] AGCGGCTCACCTCTTGGATTCAGGACATTGCTTCTTCTCGGCATTAGTAGCATTCTTAAGGATATT

[0206] CTGTCGGGTCCTGGTGTCTCTGCTAATGCATCTGTATCTCCAGCACTGAGCAGGCCCGCAGATCAG

[0207] ATCTTTGAGATAGTCAACCTAGCGAATGAGCTCCTCCCTCCATTGCCAGAAGGAGTTATCTCCCTT

[0208] CCTACTAGCACAAACACTTTGGTGAAAGGTTCAGGCCAAAAGAAATCTTCTCCAAGTACTTCAGGA

[0209] AAACAAGACGATTCACCAAAAGTTTCACCTAGAGAAAAATTACTTGCTGATCAACCTGAACTTCTG

[0210] CAGAAATTTGGACTGGATCTTCTTCCAGTTTTAGTGCAGATTTATGGTTCTAGTGTCAATGGTACT

[0211] ATTCGCCATAAATGTCTGTCAGTTATCGGAAAGTTGATGTATTTCAGCACTTCAGAAATGATTCAA

[0212] TCTCTAATTGGTGACACAAATATATCGAGCTTCTTGGCTGGTGTATTGGCATGGAAAGATCCACAA

[0213] GTCTTGGTTCCTGCTCTACAAGTTGCAGAAATTCTGATGGAAAAGCTTCCTGAAACATTCTCGAAA

[0214] GTGTTTGTGAGGGAAGGGGTGGTTCATGCTGTAGATCAACTTGTCTTGGTTGGTAAACCTATTTCT

[0215] CATGCTTCACCTACTGATAAGGAAAATGACTGTGTGCCAGGATCTGCACGATCTAGGCGATATAGA

[0216] CGGCGAAGTAGTAATGCCAGTTCTGATGGAAATCAGTCAGAAGAGCTTAAGAATTCTGCGTCGGTT

[0217] AACGTAGGTGCAAACCATAATTCCCTAGATTCTCCTACCTCTAGCTTCTTGCTAAAGGAAACAGTT

[0218] AGCTCCTGTGCCAAAGCATTCAAAGACAAGTACTTCCCGTCTGATGGCGGGGATCTCGATGTTGGA

[0219] GTTACAGATGATCTCTTACATCTAAAGAATCTTTGCACGAAGCTAACTGCTAGTATAGATGAGCAT

[0220] AAAGTGAAAGGAAAGGGAAAATCCAAAGCCTCTGGGCCATGCCTTGGCGATTTTTCTGCTAGCAAG

[0221] GAAGAATACTTGATTGGTGTCATTTCTGAGATACTTGGCGAGCTTAGCAAAGGGGATGGAGTCTCA

[0222] ACTTTTGAGTTTATTGGCAGTGGTGTGGTTGCAGCTTTGCTTGACTATTTCTCTTGTGGGTACTTT

[0223] TCCAAAGAGAAGATCTCCGAGGTTAATTTGCCCAAACTTCGCCAAGATGGGCTCAGAAGATTCAAA

[0224] GCTTTTCTAGAAGTCGCACTTCCGTCTGATGGTAATGAGGGAAAGATCCCTCCTATGACAGTTTTG

[0225] ATTCAGAAACTTCAAGATGCTTTATCTTCACTGGAACGCTTTCCCGTCGTCCTTAGTCATCCCTCA

[0226] AGGTCATTAGGTGGAAGTGCTCGTCTCTCATCTGGATTGAGTGCTTTGGCACATCCTTTAAAGTTG

[0227] CGATTATGCCGTGCACCTGGAGAGAAGACACTACGTGATTACTCCTCCAATATTGTTCTTATAGAT

[0228] CCACTGGCAAGCATTGCAGCGGTAGAAGAATTTCTTTGGCCCCGAGTTCAACGGAGTGAATCTGGT

[0229] ATGAAGCATGCAGCGCCTGCTGGAAATACAGAGCCAGGCACGTTACCTAGCGGTGCTGGTGTTTCA

[0230] TCACCATCCTCGTCAACTCCGGCTTCCACCACACGTCGTCATTCTTCTAGATCTAGATCAGCAATT

[0231] AACATAGGTGATACCTCAAAGAAAGAAGCTGTGCATGAGAAAGGTACCAGCTCATCTAAAGGAAAA

[0232] GGTAAAGGCGTTATGAAGCCCGTTCCAGCTGATAAGGGGCCTCAAACAAGGAGCAGTGCTCAAAGA

[0233] AGAGCTGTCCTTGACAAAGATACACATATGAAACCAGCTAGCGGAGACTCCAGCTCCGAGGATGAG

[0234] GAATTGGAAATAGCCCCTGTCGACATTGATGATGCCTTGGTGATTGAAGAGGATGACATGTCTGAT

[0235] GATGAAGATGATGATAATGACGATGTGTTGGATGACAGTCTTCCCATGTGCACGCCTGATAAAGTG

[0236] CATGATGTAAAATTGGGGGACGCAGTGGATGATGACGGTGGTGCTGGTCTGGCCTCTAGCGGGCGA

[0237] CAGATGAATCCAGCTTCAGGTGGCACTAGTGGAGCAGCAGCAGCGAGGGGATCTGATTCTATTGAT

[0238] GCTGGCATTGGGAATTCTTACGGTTCTAGGGGTGCACTCTCCTTTGCTGCTGCGGCAATGGCTGGG

[0239] CTTGGAGCTGCCAGTGGTAGAGGTATCAGGGGAAGTAGAGACCTGCATGGGCGTACCCTATATCGA

[0240] AGTTCCGACGAGCCCTCTAAGTTATTATTTACTGCAGGAGGAAAGCAACTTAGTAGGCATTTGACG

[0241] ATATATCAGGCTGTGCAGCGACAACTTATGCTAGATGAAGACGATGATGAAAGGTTCGGTGGCAGC

[0242] GATTTCATGTCAAGTGATGGAAGCAGATTCAATGATATTTACACCATCATGTACCAGAGGCCGGAG

[0243] AGTCAAGCGAATAGGTTGTCTGTTGGTGCAGCAAGTTCTACCACACTATCAAAATCCACTAAATCT

[0244] GCTACTGCCAATTCTAGCGTAGAATCTCAGTCATATAAGGCATCTCTTTTGGATAGTATCTTACAA

[0245] GGGGAGCTTCCGTGCGATCTTGAGAAGTCAAATTCTACTTATAATGTTCTGGCGCTGTTGCGTGTA TTAGAGGGGTTAAATCAGCTTGGCCCTCGGTTGAGAGCCCAAACTGTTTCTGATCGTTTCGCAGAG

[0246] GGTAAAATTACAAGTCTAGATGATCTGAAGACAACTGCTGCTGCTAAGATTTCTCATGAAGAATTC

[0247] ATCAATAGCAAACTTACACCCAAATTAGCTCGACAGATCCAGGATGCGCTTGCTTTGTGCAGTGGT

[0248] AGTCTTCCCTCTTGGTGCTACCAGTTGACTAGAGCATGCCCGTTTTTGTTTCCGTTTCAGACCCGG

[0249] AGACAGTATTTCTATTCAACTGCTTTTGGGTTGTCCCGCGCATTGAACCGCTTGCAGCAGCAGCAA

[0250] GGTGCTGACGGCAGTGGGTCTACAAATGAACGAGAGATGAGAATAGGGAGGTTGCAGCGCCAGAAA

[0251] GTCCGTGTATCCCGAAATAGAATATTAGATTCTGCTGCGAAAGTTATGGAGATGTATTCTAGCCAA

[0252] AAAGCTGTGCTTGAAGTAGAATATTTTGGTGAAGTTGGTACTGGTCTAGGCCCTACACTTGAGTTT

[0253] TACACACTCCTAAGCCATGATCTACAAAAGGTTGCGCTTGGGATGTGGAGATCAAATTCTGGTGAC

[0254] ATGGTATCTATGCAAATTGATAGAGGTGAGATTGAAGACGGGAAATCATCCGCAGCTAGGGACAGA

[0255] GATATAGTTCAGGCACCGCTTGGGTTGTTTCCTCGACCTTGGCCCTCTACAGCTGACGTATCTGAA

[0256] GGTAGTCGGTTTCATAAAGTCATTGAATATTTCCGGCTTTTAGGGCGTGTGATGGCAAAAGCACTT

[0257] CAAGATGGACGGCTACTGGACGTCCCATTGAGTACAGCGTTTTATAAGCTTATTCTTGGTCAAGAG

[0258] CTTGATTTGCATGATATCATATTATTTGATACCGAGCTTGGCAAGACTTTGCAAGAGTTTCGTGTT

[0259] CTTGTTGGCCGCAAGCACTATCTGGAGGCAGTAGGCGGTGACAATTGCAGCGCTGGTTCTGATTTA

[0260] TGTTTACGTGGATCCCGTATAGAAGATCTCTGCTTGGACTTCACGCTTCCTGGCTATCCTGAATAC

[0261] ATTCTGAGACCAGGAGATGAAATTGTTGATATGAATAGTCTTGAAGAGTATATATCCCTTGTCGTT

[0262] GATGCTACTGTCAAGAGAGGAGTTACCCGGCAGATTGAAGCCTTTAGATCTGGATTCAATCAGGTG

[0263] TTTGACGTAACATCTCTACAAATATTCACCCCTTCCGAGCTGGACTATTTGCTGTGTGGTCGTAGA

[0264] GAGTTGTGGGAGGCGGAGACTCTTGCTGAACATATCAAGTTTGATCACGGTTATACTGCTAAAAGT

[0265] CCGGCAATCATTAATTTACTGGAGATCATGGGAGAACTTACAGCGGATCAGCAGAGGGCTTTCTGC

[0266] CAATTTGTAACTGGAGCTCCTAGGCTTCCTCCTGGTGGGCTAGCTGTTTTGAATCCAAAGCTGACG

[0267] ATTGTGAGAAAGCATTCATCAACCTCAAGTGCGGCAGCGAACGGAACAGGGGCTTCGGAGACGGCA

[0268] GACGATGATCTTCCCAGCGTAATGACTTGCGCCAACTATCTTAAGCTCCCTCCTTATTCGACAAAG

[0269] GAAATCATGTACAAGAAACTGCTCTACGCCATCAACGAAGGGCAAGGATCGTTCGACCTGTCATAA

[0270] UPL3 Wild-Type Pennycress protein sequence ( SEQ ID NO : 6 ) :

[0271] METRSRKRAEATSTAPSSSSSSPPPPSGPTTRSKRARLSSSSSSSVAATTTAPSSSTRSRSSRSAA

[0272] AAATPMDTSTESSGFRRGGRGNRGNDNSNSDKGKEKEHDVRIRDRERERDRAREQLNMDAAAAAAA

[0273] ADDDDDNDSEDGNGGFMHPNMSSASSALQGLLRKLGAGLDDLLPSSGIGSASSSHLNGRMKKILSG

[0274] LRSEGEEGKQVEALTQLCEMLSIGTEDSLSTFSVDSFVPVLVGLLNHESNPDIMLLAARALTHLCD

[0275] VLPSSCAAVVHYGAVSCFVARLLTIEYMDLAEQSLQALKKISQEHPTACLRAGALMAVLSYLDFFS

[0276] TGVQRVALSTAANMCKKLPSDASDYVMEAVPLLTNLLQYHDSKVLEYASICLTRIAEAFASHPEKL

[0277] DELCNHGLVTQAASLISTTNSGGGQASLGVSTYTGLIRLLSTCASGSPLGFRTLLLLGISSILKDI

[0278] LSGPGVSANASVSPALSRPADQIFEIVNLANELLPPLPEGVISLPTSTNTLVKGSGQKKSSPSTSG

[0279] KQDDSPKVSPREKLLADQPELLQKFGLDLLPVLVQIYGSSVNGTIRHKCLSVIGKLMYFSTSEMIQ

[0280] SLIGDTNISSFLAGVLAWKDPQVLVPALQVAEILMEKLPETFSKVFVREGVVHAVDQLVLVGKPIS

[0281] HASPTDKENDCVPGSARSRRYRRRSSNASSDGNQSEELKNSASVNVGANHNSLDSPTSSFLLKETV

[0282] SSCAKAFKDKYFPSDGGDLDVGVTDDLLHLKNLCTKLTASIDEHKVKGKGKSKASGPCLGDFSASK

[0283] EEYLIGVISEILGELSKGDGVSTFEFIGSGVVAALLDYFSCGYFSKEKISEVNLPKLRQDGLRRFK

[0284] AFLEVALPSDGNEGKIPPMTVLIQKLQDALSSLERFPVVLSHPSRSLGGSARLSSGLSALAHPLKL

[0285] RLCRAPGEKTLRDYSSNIVLIDPLASIAAVEEFLWPRVQRSESGMKHAAPAGNTEPGTLPSGAGVS

[0286] SPSSSTPASTTRRHSSRSRSAINIGDTSKKEAVHEKGTSSSKGKGKGVMKPVPADKGPQTRSSAQR

[0287] RAVLDKDTHMKPASGDSSSEDEELEIAPVDIDDALVIEEDDMSDDEDDDNDDVLDDSLPMCTPDKV

[0288] HDVKLGDAVDDDGGAGLASSGRQMNPASGGTSGAAAARGSDSIDAGIGNSYGSRGALSFAAAAMAG

[0289] LGAASGRGIRGSRDLHGRTLYRSSDEPSKLLFTAGGKQLSRHLTIYQAVQRQLMLDEDDDERFGGS

[0290] DFMSSDGSRFNDIYTIMYQRPESQANRLSVGAASSTTLSKSTKSATANSSVESQSYKASLLDSILQ

[0291] GELPCDLEKSNSTYNVLALLRVLEGLNQLGPRLRAQTVSDRFAEGKITSLDDLKTTAAAKISHEEF

[0292] INSKLTPKLARQIQDALALCSGSLPSWCYQLTRACPFLFPFQTRRQYFYSTAFGLSRALNRLQQQQ

[0293] GADGSGSTNEREMRIGRLQRQKVRVSRNRILDSAAKVMEMYSSQKAVLEVEYFGEVGTGLGPTLEF

[0294] YTLLSHDLQKVALGMWRSNSGDMVSMQIDRGEIEDGKSSAARDRDIVQAPLGLFPRPWPSTADVSE GSRFHKVIEY FRLLGRVMAKALQDGRLLDVPLSTAFYKLILGQELDLHDI ILFDTELGKTLQE FRV LVGRKHYLEAVGGDNCSAGSDLCLRGSRIEDLCLDFTLPGYPEYILRPGDE IVDMNSLEEYISLVV DATVKRGVTRQIEAFRSGFNQVFDVTSLQI FTPSELDYLLCGRRELWEAETLAEHIKFDHGYTAKS PAI INLLEIMGELTADQQRAFCQFVTGAPRLPPGGLAVLNPKLTIVRKHSSTSSAAANGTGASETA DDDLPSVMTCANYLKLPPYSTKEIMYKKLLYAINEGQGS FDLS .

[0295] DA2 Wild-Type Pennycress ORF ( SEQ ID NO : 7 ) :

[0296] ATGGGTAATAAGTTGGGAAGGAAGAGGCAAGTGGTGGAAGAAAGGTATACAAAGCCACAAGGGTTG TATGTGAATAAAGATGTCGACGTTAAAAAGCTTAGGAAACTGATTGTAGAGTCAAAGCTTGCTCCT TGCTATCCTGGGGACGATGAAAGCTGCCACGACCTTGAAGAATGCCCGATTTGCTTCCTGTACTAT CCTAGCCTCAATAGATCAAGATGTTGCATGAAAAGCATTTGTACAGAGTGTTTTTTGCAAATGAAG AATCCTAACTCAGCTCGGCCTACTCAGTGCCCCTTTTGTAAAACACCTAATTATGCTGTCGAGTAC CGTGGAGTAAAGTCAAAGGAGGAAAAGGGCATTGAACAAGTTGAAGAGCAACGGGTTATAGAAGCC AAAAT AAGGAT G AGGC AG AAAG AAAT GO AGGAT GAT GAAGAGAAG AT GC AG AAAC GT AT GG AAT C A TGTTCCTCTAGCACAAGCGCAATGACTGGTGAAATGGAATACGGTTCAGCTTCAGCTATATCTTAT AATTCTCCTATGGAAGATGCGGAAAATGATCCATCGCAGAACGTATCAGTTGTTAGACAACAACGT TCACACCCTCGAGGAAACAGGGATGATGAGATTGACGTGGACCTTGAGGAGTTAATGGTCATGGAA GCAATATGGCTCTCCATTCAGGAAACAGGGATGCCGAGAAATAATACAGCGTGTGGGGAAATGACA CCTTTCAGGCAATATGTACCAGAAGATCATAGTTATGTTTCTTCGCCACCACGAGTGGCTCCAGTT GCAGAGCCGGAAACGCCGTCTTCATCATCTGGTGGGCTTGCTTGTGGAGTCGCTGCACTTGCTGAA CGCCAGCAAATGGTTGGCGAATCCTCCAATCACAGTCACAATCACAACCACAACGTCAACGTTTCT TCCTACAGTATGCTTCCTGGCAATTGCGACAGTTACTACGACATAGAACAAGAGGTAGATGACATC GACAACCATCATCATCATCATCATCATTACCATAATAACCACACGGAGATGGGAGGAGAAACAGGG AGCAATAGCTATGTAAGCTCTTACATGAACGGCCAGAGCTTCCACAACTTTCCTCCACCTCCTCCT CTGGTCATTGTTCCCGAGAGTTTTGAGGAGCAGATGATGATGGCTATGGCTGTGTCTTTAGCAGAG GTTCATGCCACGACCACGAGTGCGCCAACTGAAGTTACTTGGCAATAA

[0297] DA2 Wild-Type Pennycress protein sequence ( SEQ ID NO : 8 ) :

[0298] MGNKLGRKRQVVEERYTKPQGLYVNKDVDVKKLRKLIVESKLAPCYPGDDESCHDLEECPICFLYY PSLNRSRCCMKSICTECFLQMKNPNSARPTQCPFCKTPNYAVEYRGVKSKEEKGIEQVEEQRVIEA KIRMRQKEMQDDEEKMQKRMESCSSSTSAMTGEMEYGSASAISYNSPMEDAENDPSQNVSVVRQQR SHPRGNRDDE IDVDLEELMVMEAIWLSIQETGMPRNNTACGEMTPFRQYVPEDHSYVSSPPRVAPV AEPETPSSSSGGLACGVAALAERQQMVGESSNHSHNHNHNVNVSSYSMLPGNCDSYYDIEQEVDDI DNHHHHHHHYHNNHTEMGGETGSNSYVSSYMNGQSFHNFPPPPPLVIVPES FEEQMMMAMAVSLAE VHATTTSAPTEVTWQ

[0299] CRISPR Mutants da l -1 nucleic acid sequence ( +A in PSA, -AAG in PSB ) ( SEQ ID NO : 9 ) :

[0300] ATGGGTTGGTTCAACAAGATCTTCAAAGGCTCTAACCAAAGGCTCCGGGTGGGGAATGAGCACAAT

[0301] CACAATGGCTATTACGAGAACTATCCAAATGCGCATGCTTCACACGACGAGCCTAGTGCGGATACA

[0302] GATGCTGATCGTGATGAACCTCAGACGCAGGAATCATCTACATCTGAGGATACGTCGAATGACCAG

[0303] GAAAATGAAGACATAGACCGTGCAATCGCGTTGTCTCTTTTAGAAGAGAATCAAGGACAGAAAAAT

[0304] ACAGGCGGGAAATACGTAATGGTGGATGAAGATGAGCAGCTTGCTAGAGCCATACAAGAAAGTATG

[0305] ATAGTTGGGAATTCACCGCGTCACAAAAGTGGAAGTACGTATGATCATGGGAATGCATATGGGACT

[0306] GCAGATTTATTTGGGAATGGTCACATGCATGGAGGAGGAAATGTATATGCAAATGGAGATATTTAT

[0307] TACCCAAGACCCGTTGCTTTTTCAATGGATTTCAGGATTTGTGCTGGCTGCAATATGGAGATTGGC

[0308] CATGGAAGATTTCTGAATTGCCTTAACTCACTATGGCATCCAGAATGTTTTCGATGTTATGGCTGT

[0309] AGTCACCCGATTTCTGAGTACGAGTTTTCAACATCTGGGAACTACCCTTTTCACAAGGCTTGTTAC

[0310] AGGGAGAGATATCATCCAAAATGTGATGTCTGCAACCACTTTATTCCAACAAATCCTGCTGGTCTT ATTGAATATAGGGCACACCCATTTTGGATCCAGAAGTATTGCCCTTCTCACGAACACGATTCCACC

[0311] CCAAGATGTTGCAGTTGTGAAAGAATGGAGCCACGGAATACGGGATATATTGAGCTTAATGATGGA CGTAAACTTTGCCTCGAGTGTCTGGACTCAGCGGTCATGGACACAAGTCAATGTCAACCTTTGTAC TTGCAGATACAAGATTTCTATGAAGGGCTTAACAATGAAGGTAGAGCAGGAAGTTCCACTGCTCTT GGTTGAGAGGCAAGCACTTAACGAAGCCAGAGAAGGTGAAAAGAATGGTCACTATCACATGCCAGA GACAAGAGGACTGTGTCTTTCAGAAGAACAAACTGTTAGTACTATAAGAAAGCGATCAAAGGGAAA TTGGGGTGGGAATATGATCACAGAGCCGTACTTAACTCGTCAATGCGAAGTTACTGCCATTCTCAT CTTATTTGGGCTCCCTAGGCTACTGACTGGTTCGATTCTAGCTCACGAGATGATGCACGCGTGGAT GCGGCTGAAAGGATTCCGGACACTGAGCCAAGACATTGAAGAAGGTATATGTCAAGTGATGGCTCA TAAGTGGATAGAAGCCGAGTTAGCTGCTGGTTCAAGAAACAGCAGTGCTGCATCATCATCATCATC GTCATCATCTTCTTCTTCTACTAGAGGACTGAAGAAGGGACCAAAATCGCAGTACGAGAGGAAGCT TGGCGAGTTTTTCAAGCACCAGATCGAGTCTGATGCGTCTCCGGTTTATGGAGACGGGTTCAGGGC AGGGCGGCTAGCGGTTAACAAGTATGGTTTGCGAAAAACGCTTGAGCATATACAGATGACCGGTAG ATTCCCGGTTTAA

[0312] DAI-1 protein sequence ( SEQ ID NO : 10 ) :

[0313] MGWFNKIFKGSNQRLRVGNEHNHNGYYENYPNAHASHDEPSADTDADRDEPQTQESSTSEDTSNDQ ENEDIDRAIALSLLEENQGQKNTGGKYVMVDEDEQLARAIQESMIVGNSPRHKSGSTYDHGNAYGT ADLFGNGHMHGGGNVYANGDIYYPRPVAFSMDFRICAGCNMEIGHGRFLNCLNSLWHPECFRCYGC

[0314] SHPISEYEFSTSGNYPFHKACYRERYHPKCDVCNHFIPTNPAGLIEYRAHPFWIQKYCPSHEHDST

[0315] PRCCSCERMEPRNTGYIELNDGRKLCLECLDSAVMDTSQCQPLYLQIQDFYEGLN da l-2 nucleic acid sequence ( +A in PSA) ( SEQ ID NO : 11 ) :

[0316] ATGGGTTGGTTCAACAAGATCTTCAAAGGCTCTAACCAAAGGCTCCGGGTGGGGAATGAGCACAAT CACAATGGCTATTACGAGAACTATCCAAATGCGCATGCTTCACACGACGAGCCTAGTGCGGATACA GATGCTGATCGTGATGAACCTCAGACGCAGGAATCATCTACATCTGAGGATACGTCGAATGACCAG GAAAATGAAGACATAGACCGTGCAATCGCGTTGTCTCTTTTAGAAGAGAATCAAGGACAGAAAAAT ACAGGCGGGAAATACGTAATGGTGGATGAAGATGAGCAGCTTGCTAGAGCCATACAAGAAAGTATG ATAGTTGGGAATTCACCGCGTCACAAAAGTGGAAGTACGTATGATCATGGGAATGCATATGGGACT GCAGATTTATTTGGGAATGGTCACATGCATGGAGGAGGAAATGTATATGCAAATGGAGATATTTAT TACCCAAGACCCGTTGCTTTTTCAATGGATTTCAGGATTTGTGCTGGCTGCAATATGGAGATTGGC CATGGAAGATTTCTGAATTGCCTTAACTCACTATGGCATCCAGAATGTTTTCGATGTTATGGCTGT AGTCACCCGATTTCTGAGTACGAGTTTTCAACATCTGGGAACTACCCTTTTCACAAGGCTTGTTAC AGGGAGAGATATCATCCAAAATGTGATGTCTGCAACCACTTTATTCCAACAAATCCTGCTGGTCTT ATTGAATATAGGGCACACCCATTTTGGATCCAGAAGTATTGCCCTTCTCACGAACACGATTCCACC CCAAGATGTTGCAGTTGTGAAAGAATGGAGCCACGGAATACGGGATATATTGAGCTTAATGATGGA CGTAAACTTTGCCTCGAGTGTCTGGACTCAGCGGTCATGGACACAAGTCAATGTCAACCTTTGTAC TTGCAGATACAAGATTTCTATGAAGGGCTTAACAATGAAGGTAGAGCAGGAAGTTCCACTGCTCTT GGTTGAGAGGCAAGCACTTAACGAAGCCAGAGAAGGTGAAAAGAATGGTCACTATCACATGCCAGA GACAAGAGGACTGTGTCTTTCAGAAGAACAAACTGTTAGTACTATAAGAAAGCGATCAAAGGGAAA TTGGGGTGGGAATATGATCACAGAGCCGTACAAGTTAACTCGTCAATGCGAAGTTACTGCCATTCT CATCTTATTTGGGCTCCCTAGGCTACTGACTGGTTCGATTCTAGCTCACGAGATGATGCACGCGTG GATGCGGCTGAAAGGATTCCGGACACTGAGCCAAGACATTGAAGAAGGTATATGTCAAGTGATGGC TCATAAGTGGATAGAAGCCGAGTTAGCTGCTGGTTCAAGAAACAGCAGTGCTGCATCATCATCATC ATCGTCATCATCTTCTTCTTCTACTAGAGGACTGAAGAAGGGACCAAAATCGCAGTACGAGAGGAA GCTTGGCGAGTTTTTCAAGCACCAGATCGAGTCTGATGCGTCTCCGGTTTATGGAGACGGGTTCAG GGCAGGGCGGCTAGCGGTTAACAAGTATGGTTTGCGAAAAACGCTTGAGCATATACAGATGACCGG TAGATTCCCGGTTTAA DAI-2 protein sequence ( SEQ ID NO : 12 ) :

[0317] MGWFNKIFKGSNQRLRVGNEHNHNGYYENYPNAHASHDEPSADTDADRDEPQTQESSTSEDTSNDQ

[0318] ENEDIDRAIALSLLEENQGQKNTGGKYVMVDEDEQLARAIQESMIVGNSPRHKSGSTYDHGNAYGT

[0319] ADLFGNGHMHGGGNVYANGDIYYPRPVAFSMDFRICAGCNMEIGHGRFLNCLNSLWHPECFRCYGC

[0320] SHPISEYEFSTSGNYPFHKACYRERYHPKCDVCNHFIPTNPAGLIEYRAHPFWIQKYCPSHEHDST PRCCSCERMEPRNTGYIELNDGRKLCLECLDSAVMDTSQCQPLYLQIQDFYEGLN da l-3 nucleic acid sequence ( -285bp between PSA and PSB ) ( SEQ ID

[0321] NO : 13 ) :

[0322] ATGGGTTGGTTCAACAAGATCTTCAAAGGCTCTAACCAAAGGCTCCGGGTGGGGAATGAGCACAAT

[0323] CACAATGGCTATTACGAGAACTATCCAAATGCGCATGCTTCACACGACGAGCCTAGTGCGGATACA

[0324] GATGCTGATCGTGATGAACCTCAGACGCAGGAATCATCTACATCTGAGGATACGTCGAATGACCAG

[0325] GAAAATGAAGACATAGACCGTGCAATCGCGTTGTCTCTTTTAGAAGAGAATCAAGGACAGAAAAAT

[0326] ACAGGCGGGAAATACGTAATGGTGGATGAAGATGAGCAGCTTGCTAGAGCCATACAAGAAAGTATG

[0327] ATAGTTGGGAATTCACCGCGTCACAAAAGTGGAAGTACGTATGATCATGGGAATGCATATGGGACT

[0328] GCAGATTTATTTGGGAATGGTCACATGCATGGAGGAGGAAATGTATATGCAAATGGAGATATTTAT

[0329] TACCCAAGACCCGTTGCTTTTTCAATGGATTTCAGGATTTGTGCTGGCTGCAATATGGAGATTGGC

[0330] CATGGAAGATTTCTGAATTGCCTTAACTCACTATGGCATCCAGAATGTTTTCGATGTTATGGCTGT

[0331] AGTCACCCGATTTCTGAGTACGAGTTTTCAACATCTGGGAACTACCCTTTTCACAAGGCTTGTTAC

[0332] AGGGAGAGATATCATCCAAAATGTGATGTCTGCAACCACTTTATTCCAACAAATCCTGCTGGTCTT

[0333] ATTGAATATAGGGCACACCCATTTTGGATCCAGAAGTATTGCCCTTCTCACGAACACGATTCCACC

[0334] CCAAGATGTTGCAGTTGTGAAAGAATGGAGCCACGGAATACGGGATATATTGAGCTTAATGATGGA

[0335] CGTAAACTTTGCCTCGAGTGTCTGGACTCAGCGGTCATGGACACAAGTCAATGTCAACCTTTGTAC

[0336] TTGCAGATACAAGATTTCTATGAAGGGCTTAACAAGTTAACTCGTCAATGCGAAGTTACTGCCATT

[0337] CTCATCTTATTTGGGCTCCCTAGGCTACTGACTGGTTCGATTCTAGCTCACGAGATGATGCACGCG

[0338] TGGATGCGGCTGAAAGGATTCCGGACACTGAGCCAAGACATTGAAGAAGGTATATGTCAAGTGATG

[0339] GCTCATAAGTGGATAGAAGCCGAGTTAGCTGCTGGTTCAAGAAACAGCAGTGCTGCATCATCATCA

[0340] TCATCGTCATCATCTTCTTCTTCTACTAGAGGACTGAAGAAGGGACCAAAATCGCAGTACGAGAGG

[0341] AAGCTTGGCGAGTTTTTCAAGCACCAGATCGAGTCTGATGCGTCTCCGGTTTATGGAGACGGGTTC

[0342] AGGGCAGGGCGGCTAGCGGTTAACAAGTATGGTTTGCGAAAAACGCTTGAGCATATACAGATGACC GGTAGATTCCCGGTTTAA

[0343] DAI-3 protein sequence ( SEQ ID NO : 14 ) :

[0344] MGWFNKIFKGSNQRLRVGNEHNHNGYYENYPNAHASHDEPSADTDADRDEPQTQESSTSEDTSNDQ

[0345] ENEDIDRAIALSLLEENQGQKNTGGKYVMVDEDEQLARAIQESMIVGNSPRHKSGSTYDHGNAYGT

[0346] ADLFGNGHMHGGGNVYANGDIYYPRPVAFSMDFRICAGCNMEIGHGRFLNCLNSLWHPECFRCYGC

[0347] SHPISEYEFSTSGNYPFHKACYRERYHPKCDVCNHFIPTNPAGLIEYRAHPFWIQKYCPSHEHDST PRCCSCERMEPRNTGYIELNDGRKLCLECLDSAVMDTSQCQPLYLQIQDFYEGLN darl -1 nucleic acid sequence ( -857bp between PS3 and PS4 ) ( SEQ ID

[0348] NO : 15 ) :

[0349] ATGGGGTGGCTTACTAAAATCCTTAAAGGCTCAAGCCATAAATTCTCGGATGGACAAGTTAACAGA

[0350] AGATATAGAGATGATAGGAACTTGGAAAGTTCTCGTCTTTCTGCGGATGGATCTGATTTTGACAAA

[0351] GAAGAGATTGAATGTGCTATTGCACTCTCTCTTTCAGAACAAGAACATGTAATTCCACAAGATGAC

[0352] AAAGGAAAACAAGTAATCGAATACAAATCTGAAACCGAAGAAGATGAGGATGAGGATGAGGACGAA

[0353] GATGAAGAATACACGAGAGCTCAGCTAGAAGCAGCGGAAGAAGAGGAAAGACGGGCAGCTCAAGCT

[0354] CAAATCGAGGAAGAAGAGAAACGGCGAGCTGAAGCTGAATTAGAAGAAATAGAGAAACAGCTTGCG

[0355] AAAGCTCGACTAGAAGAGGAAGAAGTTAGGCGTACCAAAGCGCAATTGGAGGAAGACGAGCAACTA

[0356] GCTAAGGCCATTCAAGAAAGTATGGTTGTGGGATCTCCTCCTCGGTATGATTCTGGAAGTATGTTT

[0357] CAACCATTCCCTTTCCTTAATCCATCTAGTCACAGGGGTTGCCTAGCTGAGATTGGACATGGAAGG

[0358] TTTCTGAGTTGCATGGGTGGCGTTTGGCATCCTGAATGTTTCTGCTGCCATGCTTGTGATAAGCCC AT C AT AGAT T AT GAGT T C T C AAT GT C CGGG AAT TGGGGCAGGC T AC AAAT T GAT AGAC AT GAT C AC AGAGCCTTGCAGGCTGATACGTCGTTGTGAAGTCACTGCAATTCTCATTCTATATGGACTTCCTCG TTTGTTAACTGGATCAATCCTTGCTCATGAGATGATGCATGCCTGGCTTCGACTAAATGGtaaGTA TCCGAATCTTAGGCCAGAAGTTGAAGAAGGAATATGTCAGGTTTTGGCTCACATGTGGTTGGAATC TGAGACTTATGCTGGCTCTACATTAGTAGATATCGCATCTTCTTCATCATCATCATCATCGGCTGT GGTCACTGCATCGTCAAAGAAAGGTGAGAGGTCTGACTTTGAGAAGAAACTTGGTGAGTTTTTCAA GCACCAAATAGAGTCGGATTCTTCGTCGGCATATGGGGATGGGTTCAGGCAAGGTAACCAAGCAGT TCTTACGCACGGTCTGAGGCGAACCCTCGACCATATTCGCTTGACCGGTACATTTCCTTAA

[0359] DARI -1 protein sequence ( SEQ ID NO : 16 ) :

[0360] MGWLTKILKGSSHKFSDGQVNRRYRDDRNLESSRLSADGSDFDKEEIECAIALSLSEQEHVIPQDD KGKQVIEYKSETEEDEDEDEDEDEEYTRAQLEAAEEEERRAAQAQIEEEEKRRAEAELEEIEKQLA KARLEEEEVRRTKAQLEEDEQLAKAIQESMVVGSPPRYDSGSMFQPFPFLNPSSHRGCLAEIGHGR FLSCMGGVWHPECFCCHACDKPI IDYEFSMSGN darl -2 nucleic acid sequence ( -4bp in PS3 , -A in PS 4 ) ( SEQ ID

[0361] NO : 17 ) :

[0362] ATGGGGTGGCTTACTAAAATCCTTAAAGGCTCAAGCCATAAATTCTCGGATGGACAAGTTAACAGA AGATATAGAGATGATAGGAACTTGGAAAGTTCTCGTCTTTCTGCGGATGGATCTGATTTTGACAAA GAAGAGATTGAATGTGCTATTGCACTCTCTCTTTCAGAACAAGAACATGTAATTCCACAAGATGAC AAAGGAAAACAAGTAATCGAATACAAATCTGAAACCGAAGAAGATGAGGATGAGGATGAGGACGAA GATGAAGAATACACGAGAGCTCAGCTAGAAGCAGCGGAAGAAGAGGAAAGACGGGCAGCTCAAGCT CAAATCGAGGAAGAAGAGAAACGGCGAGCTGAAGCTGAATTAGAAGAAATAGAGAAACAGCTTGCG AAAGCTCGACTAGAAGAGGAAGAAGTTAGGCGTACCAAAGCGCAATTGGAGGAAGACGAGCAACTA GCTAAGGCCATTCAAGAAAGTATGGTTGTGGGATCTCCTCCTCGGTATGATTCTGGAAGTATGTTT CAACCATTCCCTTTCCTTAATCCATCTAGTCACAGGGGTTGCCTAGCTGAGATTGGACATGGAAGG TTTCTGAGTTGCATGGGTGGCGTTTGGCATCCTGAATGTTTCTGCTGCCATGCTTGTGATAAGCCC ATCATAGATTATGAGTTCTCAATGTCCGGGAGCCTTATCACAAGCTATGTTACAAGGAGCAGCATC ATCCTAAATGTGATGTTTGCCATAACTTTATTCCGACGAATTCAGCTGGTCTCATTGAGTACAGGG CACATCCCTTTTGGATGCAAAAGTATTGTCCTTCACATGAGCGTGATGGAACTCCTCGTTGCTGTA GCTGTGAGCGAATGGAGCCAAAAGATACAAAATATCTCATTCTTGATGATGGTCGAAAACTGTGTC TTGAATGTCTCGACTCAGCCATTATGGACACTCACGAATGCCAACCATTGTACCTTGAGATACGTG AGTTTTATGAAGGCTTACACATGAAAGTAGAACAGCAGATTCCTATGCTCTTGGTGGAGAGATCAG CTTTAAACGAAGCTATGGAAGGAGAGAAACACGGACATCATCATTTACCTGAAACCAGAGGACTCT GT TT GT CT GAGGAACAAACTGT CACAACGGTGTT AAGGAGACC AAGATT GGGGCAGGCT AC AAAT T

[0363] GATAGACATGATCACAGAGCCTTGCAGGCTGATACGTCGTTGTGAAGTCACTGCAATTCTCATTCT ATATGGACTTCCTCGTTTGTTAACTGGATCAATCCTTGCTCATGAGATGATGCATGCCTGGCTTCG ACTAAATGGtaaGTATCCGAATCTTAGGCCAGAAGTTGAAGAAGGAATATGTCAGGTTTTGGCTCA CATGTGGTTGGAATCTGAGACTTATGCTGGCTCTACATTAGTAGATATCGCATCTTCTTCATCATC ATCATCATCGGCTGTGGTCACTGCATCGTCAAAGAAAGGTGAGAGGTCTGACTTTGAGAAGAAACT TGGTGAGTTTTTCAAGCACCAAATAGAGTCGGATTCTTCGTCGGCATATGGGGATGGGTTCAGGCA AGGTAACCAAGCAGTTCTTACGCACGGTCTGAGGCGAACCCTCGACCATATTCGCTTGACCGGTAC

[0364] DARI -2 protein sequence ( SEQ ID NO : 18 ) :

[0365] MGWLTKILKGSSHKFSDGQVNRRYRDDRNLESSRLSADGSDFDKEEIECAIALSLSEQEHVIPQDD

[0366] KGKQVIEYKSETEEDEDEDEDEDEEYTRAQLEAAEEEERRAAQAQIEEEEKRRAEAELEEIEKQLA

[0367] KARLEEEEVRRTKAQLEEDEQLAKAIQESMVVGSPPRYDSGSMFQPFPFLNPSSHRGCLAEIGHGR

[0368] FLSCMGGVWHPECFCCHACDKPI IDYEFSMSG darl -3 nucleic acid sequence ( -2bp in PS5 ) ( SEQ ID NO : 19 ) :

[0369] ATGGGGTGGCTTACTAAAATCCTTAAAGGCTCAAGCCATAAATTCTCGGATGGACAAGTTAACAGA AGATATAGAGATGATAGGAACTTGGAAAGTTCTCGTCTTTCTGCGGATGGATCTGATTTTGACAAA GAAGAGATTGAATGTGCTATTGCACTCTCTCTTTCAGAACAAGAACATGTAATTCCACAAGATGAC AAAGGAAAACAAGTAATCGAATACAAATCTGAAACCGAAGAAGATGAGGATGAGGATGAGGACGAA GATGAAGAATACACGAGAGCTCAGCTAGAAGCAGCGGAAGAAGAGGAAAGACGGGCAGCTCAAGCT CAAATCGAGGAAGAAGAGAAACGGCGAGCTGAAGCTGAATTAGAAGAAATAGAGAAACAGCTTGCG AAAGCTCGACTAGAAGAGGAAGAAGTTAGGCGTACCAAAGCGCAATTGGAGGAAGACGAGCAACTA GCTAAGGCCATTCAAGAAAGTATGGTTGTGGGATCTCCTCCTCGGTATGATTCTGGAAGTATGTTT CAACCATTCCCTTTCCTTAATCCATCTAGTCACAGGGGTTGCCTAGCTGAGATTGGACATGGAAGG TTTCTGAGTTGCATGGGTGGCGTTTGGCATCCTGAATGTTTCTGCTGCCATGCTTGTGATAAGCCC ATCATAGATTATGAGTTCTCAATGTCCGGGAATCGGCCTTATCACAAGCTATGTTACAAGGAGCAG CATCATCCTAAATGTGATGTTTGCCATAACTTTATTCCGACGAATTCAGCTGGTCTCATTGAGTAC AGGGCACATCCCTTTTGGATGCAAAAGTATTGTCCTTCACATGAGCGTGATGGAACTCCTCGTTGC TGTAGCTGTGAGCGAATGGAGCCAAAAGATACAAAATATCTCATTCTTGATGATGGTCGAAAACTG TGTCTTGAATGTCTCGACTCAGCCATTATGGACACTCACGAATGCCAACCATTGTACCTTGAGATA CGTGAGTTTTATGAAGGCTTACACATGAAAGTAGAACAGCAGATTCCTATGCTCTTGGTGGAGAGA TCAGCTTTAAACGAAGCTATGGAAGGAGAGAAACACGGACATCATCATTTACCTGAAACCAGAGGA CTCTGTTTGTCTGAGGAACAAACTGTCACAACGGTGTTAAGGAGACCAAGAATTGGGGCAGGCTAC AAATTGATAGACATGATCACAGAGCCTTGCAGGCTGATACGTCGTTGTGAAGTCACTGCAATTCTC ATTCTATATGGACTTCCTCGTTTGTTAACTGGATCAATCCTTGCTCATGAGATGATGCATGCCTGG TCGACTAAATGGtaaGTATCCGAATCTTAGGCCAGAAGTTGAAGAAGGAATATGTCAGGTTTTGGC TCACATGTGGTTGGAATCTGAGACTTATGCTGGCTCTACATTAGTAGATATCGCATCTTCTTCATC ATCATCATCATCGGCTGTGGTCACTGCATCGTCAAAGAAAGGTGAGAGGTCTGACTTTGAGAAGAA ACTTGGTGAGTTTTTCAAGCACCAAATAGAGTCGGATTCTTCGTCGGCATATGGGGATGGGTTCAG GCAAGGTAACCAAGCAGTTCTTACGCACGGTCTGAGGCGAACCCTCGACCATATTCGCTTGACCGG TACATTTCCTTAA

[0370] DARl-3_protein sequence ( SEQ ID NO : 20 ) :

[0371] MGWLTKILKGSSHKFSDGQVNRRYRDDRNLESSRLSADGSDFDKEEIECAIALSLSEQEHVIPQDD

[0372] KGKQVIEYKSETEEDEDEDEDEDEEYTRAQLEAAEEEERRAAQAQIEEEEKRRAEAELEEIEKQLA

[0373] KARLEEEEVRRTKAQLEEDEQLAKAIQESMVVGSPPRYDSGSMFQPFPFLNPSSHRGCLAEIGHGR

[0374] FLSCMGGVWHPECFCCHACDKPIIDYEFSMSGNRPYHKLCYKEQHHPKCDVCHNFIPTNSAGLIEY

[0375] RAHPFWMQKYCPSHERDGTPRCCSCERMEPKDTKYLILDDGRKLCLECLDSAIMDTHECQPLYLEI

[0376] REFYEGLHMKVEQQIPMLLVERSALNEAMEGEKHGHHHLPETRGLCLSEEQTVTTVLRRPRIGAGY KLIDMITEPCRLIRRCEVTAILILYGLPRLLTGSILAHEMMHAW

[0377] (daldarl) -l double mutant nucleic acid sequences :

[0378] DAI ( Inversion of 284bp between PSA and PSB , +T in PSB ) ( SEQ ID

[0379] NO : 21 ) :

[0380] ATGGGTTGGTTCAACAAGATCTTCAAAGGCTCTAACCAAAGGCTCCGGGTGGGGAATGAGCACAAT

[0381] CACAATGGCTATTACGAGAACTATCCAAATGCGCATGCTTCACACGACGAGCCTAGTGCGGATACA

[0382] GATGCTGATCGTGATGAACCTCAGACGCAGGAATCATCTACATCTGAGGATACGTCGAATGACCAG

[0383] GAAAATGAAGACATAGACCGTGCAATCGCGTTGTCTCTTTTAGAAGAGAATCAAGGACAGAAAAAT

[0384] ACAGGCGGGAAATACGTAATGGTGGATGAAGATGAGCAGCTTGCTAGAGCCATACAAGAAAGTATG

[0385] ATAGTTGGGAATTCACCGCGTCACAAAAGTGGAAGTACGTATGATCATGGGAATGCATATGGGACT

[0386] GCAGATTTATTTGGGAATGGTCACATGCATGGAGGAGGAAATGTATATGCAAATGGAGATATTTAT

[0387] TACCCAAGACCCGTTGCTTTTTCAATGGATTTCAGGATTTGTGCTGGCTGCAATATGGAGATTGGC

[0388] CATGGAAGATTTCTGAATTGCCTTAACTCACTATGGCATCCAGAATGTTTTCGATGTTATGGCTGT

[0389] AGTCACCCGATTTCTGAGTACGAGTTTTCAACATCTGGGAACTACCCTTTTCACAAGGCTTGTTAC

[0390] AGGGAGAGATATCATCCAAAATGTGATGTCTGCAACCACTTTATTCCAACAAATCCTGCTGGTCTT ATTGAATATAGGGCACACCCATTTTGGATCCAGAAGTATTGCCCTTCTCACGAACACGATTCCACC CCAAGATGTTGCAGTTGTGAAAGAATGGAGCCACGGAATACGGGATATATTGAGCTTAATGATGGA CGTAAACTTTGCCTCGAGTGTCTGGACTCAGCGGTCATGGACACAAGTCAATGTCAACCTTTGTAC TTGCAGATACAAGATTTCTATGAAGGGCTTAACAGTACGGCTCTGTGATCATATTCCCACCCCAAT TTCCCTTTGATCGCTTTCTTATAGTACTAACAGTTTGTTCTTCTGAAAGACACAGTCCTCTTGTCT CTGGCATGTGATAGTGACCCTAAGAGGCACACAATGAAAAACTCAGGAGAAGCAAAATGTGATGTA TATTCAAGTAATTACTAAGTAATGATTGTATTTTTTTACTCACATTCTTTTCACCTTCTCTGGCTT CGTTAAGTGCTTGCCTCTCAACCAAGAGCAGTGGAACTTCCTGCTCTACCTTCATAAGTTAACTCG TCAATGCGAAGTTACTGCCATTCTCATCTTATTTGGGCTCCCTAGGCTACTGACTGGTTCGATTCT AGCTCACGAGATGATGCACGCGTGGATGCGGCTGAAAGGATTCCGGACACTGAGCCAAGACATTGA AGAAGGTATATGTCAAGTGATGGCTCATAAGTGGATAGAAGCCGAGTTAGCTGCTGGTTCAAGAAA CAGCAGTGCTGCATCATCATCATCATCGTCATCATCTTCTTCTTCTACTAGAGGACTGAAGAAGGG ACCAAAATCGCAGTACGAGAGGAAGCTTGGCGAGTTTTTCAAGCACCAGATCGAGTCTGATGCGTC TCCGGTTTATGGAGACGGGTTCAGGGCAGGGCGGCTAGCGGTTAACAAGTATGGTTTGCGAAAAAC GCTTGAGCATATACAGATGACCGGTAGATTCCCGGTTTAA

[0391] DARI ( -857bp between PS3 and PS4 ) ( SEQ ID NO : 23 ) :

[0392] ATGGGGTGGCTTACTAAAATCCTTAAAGGCTCAAGCCATAAATTCTCGGATGGACAAGTTAACAGA AGATATAGAGATGATAGGAACTTGGAAAGTTCTCGTCTTTCTGCGGATGGATCTGATTTTGACAAA GAAGAGATTGAATGTGCTATTGCACTCTCTCTTTCAGAACAAGAACATGTAATTCCACAAGATGAC AAAGGAAAACAAGTAATCGAATACAAATCTGAAACCGAAGAAGATGAGGATGAGGATGAGGACGAA GATGAAGAATACACGAGAGCTCAGCTAGAAGCAGCGGAAGAAGAGGAAAGACGGGCAGCTCAAGCT CAAATCGAGGAAGAAGAGAAACGGCGAGCTGAAGCTGAATTAGAAGAAATAGAGAAACAGCTTGCG AAAGCTCGACTAGAAGAGGAAGAAGTTAGGCGTACCAAAGCGCAATTGGAGGAAGACGAGCAACTA GCTAAGGCCATTCAAGAAAGTATGGTTGTGGGATCTCCTCCTCGGTATGATTCTGGAAGTATGTTT CAACCATTCCCTTTCCTTAATCCATCTAGTCACAGGGGTTGCCTAGCTGAGATTGGACATGGAAGG TTTCTGAGTTGCATGGGTGGCGTTTGGCATCCTGAATGTTTCTGCTGCCATGCTTGTGATAAGCCC AT C AT AGAT T AT GAGT T C T C AAT GT C CGGG AAT TGGGGCAGGC T AC AAAT T GAT AGAC AT GAT C AC AGAGCCTTGCAGGCTGATACGTCGTTGTGAAGTCACTGCAATTCTCATTCTATATGGACTTCCTCG TTTGTTAACTGGATCAATCCTTGCTCATGAGATGATGCATGCCTGGCTTCGACTAAATGGtaaGTA TCCGAATCTTAGGCCAGAAGTTGAAGAAGGAATATGTCAGGTTTTGGCTCACATGTGGTTGGAATC TGAGACTTATGCTGGCTCTACATTAGTAGATATCGCATCTTCTTCATCATCATCATCATCGGCTGT GGTCACTGCATCGTCAAAGAAAGGTGAGAGGTCTGACTTTGAGAAGAAACTTGGTGAGTTTTTCAA GCACCAAATAGAGTCGGATTCTTCGTCGGCATATGGGGATGGGTTCAGGCAAGGTAACCAAGCAGT TCTTACGCACGGTCTGAGGCGAACCCTCGACCATATTCGCTTGACCGGTACATTTCCTTAA

[0393] ( DAlDARl ) - l double mutant protein sequences :

[0394] DAI ( SEQ ID NO : 22 ) :

[0395] MGWFNKI FKGSNQRLRVGNEHNHNGYYENYPNAHASHDEPSADTDADRDEPQTQESSTSEDTSNDQ ENEDIDRAIALSLLEENQGQKNTGGKYVMVDEDEQLARAIQESMIVGNSPRHKSGSTYDHGNAYGT ADLFGNGHMHGGGNVYANGDIYYPRPVAFSMDFRICAGCNMEIGHGRFLNCLNSLWHPECFRCYGC SHPI SEYEFSTSGNYPFHKACYRERYHPKCDVCNHFIPTNPAGLIEYRAHPFWIQKYCPSHEHDST PRCCSCERMEPRNTGYIELNDGRKLCLECLDSAVMDTSQCQPLYLQIQDFYEGLN

[0396] DARI ( SEQ ID NO : 24 ) :

[0397] MGWLTKILKGSSHKFSDGQVNRRYRDDRNLESSRLSADGSDFDKEEIECAIALSLSEQEHVIPQDD KGKQVIEYKSETEEDEDEDEDEDEEYTRAQLEAAEEEERRAAQAQIEEEEKRRAEAELEEIEKQLA KARLEEEEVRRTKAQLEEDEQLAKAIQESMVVGSPPRYDSGSMFQPFPFLNPSSHRGCLAEIGHGR FLSCMGGVWHPECFCCHACDKPI IDYEFSMSGN ( da ldarl ) -2 double mutant nucleic acid sequences : da l ( -297bp between PSA and PSB ) ( SEQ ID NO : 73 ) :

[0398] ATGGGTTGGTTCAACAAGATCTTCAAAGGCTCTAACCAAAGGCTCCGGGTGGGGAATGAGCACAAT CACAATGGCTATTACGAGAACTATCCAAATGCGCATGCTTCACACGACGAGCCTAGTGCGGATACA GATGCTGATCGTGATGAACCTCAGACGCAGGAATCATCTACATCTGAGGATACGTCGAATGACCAG GAAAATGAAGACATAGACCGTGCAATCGCGTTGTCTCTTTTAGAAGAGAATCAAGGACAGAAAAAT ACAGGCGGGAAATACGTAATGGTGGATGAAGATGAGCAGCTTGCTAGAGCCATACAAGAAAGTATG ATAGTTGGGAATTCACCGCGTCACAAAAGTGGAAGTACGTATGATCATGGGAATGCATATGGGACT GCAGATTTATTTGGGAATGGTCACATGCATGGAGGAGGAAATGTATATGCAAATGGAGATATTTAT TACCCAAGACCCGTTGCTTTTTCAATGGATTTCAGGATTTGTGCTGGCTGCAATATGGAGATTGGC CATGGAAGATTTCTGAATTGCCTTAACTCACTATGGCATCCAGAATGTTTTCGATGTTATGGCTGT AGTCACCCGATTTCTGAGTACGAGTTTTCAACATCTGGGAACTACCCTTTTCACAAGGCTTGTTAC AGGGAGAGATATCATCCAAAATGTGATGTCTGCAACCACTTTATTCCAACAAATCCTGCTGGTCTT ATTGAATATAGGGCACACCCATTTTGGATCCAGAAGTATTGCCCTTCTCACGAACACGATTCCACC CCAAGATGTTGCAGTTGTGAAAGAATGGAGCCACGGAATACGGGATATATTGAGCTTAATGATGGA CGTAAACTTTGCCTCGAGTGTCTGGACTCAGCGGTCATGGACACAAGTCAATGTCAACCTTTGTAC TTGCAGATACAAGATTTCTATGAAGGGCCTCGTCAATGCGAAGTTACTGCCATTCTCATCTTATTT GGGCTCCCTAGGCTACTGACTGGTTCGATTCTAGCTCACGAGATGATGCACGCGTGGATGCGGCTG AAAGGATTCCGGACACTGAGCCAAGACATTGAAGAAGGTATATGTCAAGTGATGGCTCATAAGTGG ATAGAAGCCGAGTTAGCTGCTGGTTCAAGAAACAGCAGTGCTGCATCATCATCATCATCGTCATCA TCTTCTTCTTCTACTAGAGGACTGAAGAAGGGACCAAAATCGCAGTACGAGAGGAAGCTTGGCGAG TTTTTCAAGCACCAGATCGAGTCTGATGCGTCTCCGGTTTATGGAGACGGGTTCAGGGCAGGGCGG CTAGCGGTTAACAAGTATGGTTTGCGAAAAACGCTTGAGCATATACAGATGACCGGTAGATTCCCG GTTTAA darl ( -857bp between PS3 and PS4 ) ( SEQ ID NO : 75 )

[0399] ATGGGGTGGCTTACTAAAATCCTTAAAGGCTCAAGCCATAAATTCTCGGATGGACAAGTTAACAGA AGATATAGAGATGATAGGAACTTGGAAAGTTCTCGTCTTTCTGCGGATGGATCTGATTTTGACAAA GAAGAGATTGAATGTGCTATTGCACTCTCTCTTTCAGAACAAGAACATGTAATTCCACAAGATGAC AAAGGAAAACAAGTAATCGAATACAAATCTGAAACCGAAGAAGATGAGGATGAGGATGAGGACGAA GATGAAGAATACACGAGAGCTCAGCTAGAAGCAGCGGAAGAAGAGGAAAGACGGGCAGCTCAAGCT CAAATCGAGGAAGAAGAGAAACGGCGAGCTGAAGCTGAATTAGAAGAAATAGAGAAACAGCTTGCG AAAGCTCGACTAGAAGAGGAAGAAGTTAGGCGTACCAAAGCGCAATTGGAGGAAGACGAGCAACTA GCTAAGGCCATTCAAGAAAGTATGGTTGTGGGATCTCCTCCTCGGTATGATTCTGGAAGTATGTTT CAACCATTCCCTTTCCTTAATCCATCTAGTCACAGGGGTTGCCTAGCTGAGATTGGACATGGAAGG TTTCTGAGTTGCATGGGTGGCGTTTGGCATCCTGAATGTTTCTGCTGCCATGCTTGTGATAAGCCC AT C AT AGAT T AT GAGT T C T C AAT GT C CGGG AAT TGGGGCAGGC T AC AAAT T GAT AGAC AT GAT GAG AGAGCCTTGCAGGCTGATACGTCGTTGTGAAGTCACTGCAATTCTCATTCTATATGGACTTCCTCG TTTGTTAACTGGATCAATCCTTGCTCATGAGATGATGCATGCCTGGCTTCGACTAAATGGtaaGTA TCCGAATCTTAGGCCAGAAGTTGAAGAAGGAATATGTCAGGTTTTGGCTCACATGTGGTTGGAATC TGAGACTTATGCTGGCTCTACATTAGTAGATATCGCATCTTCTTCATCATCATCATCATCGGCTGT GGTCACTGCATCGTCAAAGAAAGGTGAGAGGTCTGACTTTGAGAAGAAACTTGGTGAGTTTTTCAA GCACCAAATAGAGTCGGATTCTTCGTCGGCATATGGGGATGGGTTCAGGCAAGGTAACCAAGCAGT TCTTACGCACGGTCTGAGGCGAACCCTCGACCATATTCGCTTGACCGGTACATTTCCTTAA

[0400] ( DAlDARl ) -2 double mutant protein sequences :

[0401] DAI ( SEQ ID NO : 74 ) :

[0402] MGWFNKI FKGSNQRLRVGNEHNHNGYYENYPNAHASHDEPSADTDADRDEPQTQESSTSEDTSNDQ ENEDIDRAIALSLLEENQGQKNTGGKYVMVDEDEQLARAIQESMIVGNSPRHKSGSTYDHGNAYGT ADLFGNGHMHGGGNVYANGDIYYPRPVAFSMDFRICAGCNMEIGHGRFLNCLNSLWHPECFRCYGC SHPI SEYEFSTSGNYPFHKACYRERYHPKCDVCNHFIPTNPAGLIEYRAHPFWIQKYCPSHEHDST PRCCSCERMEPRNTGYIELNDGRKLCLECLDSAVMDTSQCQPLYLQIQDFYEGPRQCEVTAILILF

[0403] GLPRLLTGSILAHEMMHAWMRLKGFRTLSQDIEEGICQVMAHKWIEAELAAGSRNSSAASSSSSSS

[0404] SSSSTRGLKKGPKSQYERKLGEFFKHQIESDASPVYGDGFRAGRLAVNKYGLRKTLEHIQMTGRFP V

[0405] DARI ( SEQ ID NO : 76 ) :

[0406] MGWLTKILKGSSHKFSDGQVNRRYRDDRNLESSRLSADGSDFDKEEIECAIALSLSEQEHVIPQDD

[0407] KGKQVIEYKSETEEDEDEDEDEDEEYTRAQLEAAEEEERRAAQAQIEEEEKRRAEAELEEIEKQLA

[0408] KARLEEEEVRRTKAQLEEDEQLAKAIQESMVVGSPPRYDSGSMFQPFPFLNPSSHRGCLAEIGHGR FLSCMGGVWHPECFCCHACDKPIIDYEFSMSGN

[0409] ( daldarl ) -3 double mutant nucleic acid sequences : da l ( inversion of 283bp between PSA and PSB ) ( SEQ ID NO : 77 ) :

[0410] ATGGGTTGGTTCAACAAGATCTTCAAAGGCTCTAACCAAAGGCTCCGGGTGGGGAATGAGCACAAT

[0411] CACAATGGCTATTACGAGAACTATCCAAATGCGCATGCTTCACACGACGAGCCTAGTGCGGATACA

[0412] GATGCTGATCGTGATGAACCTCAGACGCAGGAATCATCTACATCTGAGGATACGTCGAATGACCAG

[0413] GAAAATGAAGACATAGACCGTGCAATCGCGTTGTCTCTTTTAGAAGAGAATCAAGGACAGAAAAAT

[0414] ACAGGCGGGAAATACGTAATGGTGGATGAAGATGAGCAGCTTGCTAGAGCCATACAAGAAAGTATG

[0415] ATAGTTGGGAATTCACCGCGTCACAAAAGTGGAAGTACGTATGATCATGGGAATGCATATGGGACT

[0416] GCAGATTTATTTGGGAATGGTCACATGCATGGAGGAGGAAATGTATATGCAAATGGAGATATTTAT

[0417] TACCCAAGACCCGTTGCTTTTTCAATGGATTTCAGGATTTGTGCTGGCTGCAATATGGAGATTGGC

[0418] CATGGAAGATTTCTGAATTGCCTTAACTCACTATGGCATCCAGAATGTTTTCGATGTTATGGCTGT

[0419] AGTCACCCGATTTCTGAGTACGAGTTTTCAACATCTGGGAACTACCCTTTTCACAAGGCTTGTTAC

[0420] AGGGAGAGATATCATCCAAAATGTGATGTCTGCAACCACTTTATTCCAACAAATCCTGCTGGTCTT

[0421] ATTGAATATAGGGCACACCCATTTTGGATCCAGAAGTATTGCCCTTCTCACGAACACGATTCCACC

[0422] CCAAGATGTTGCAGTTGTGAAAGAATGGAGCCACGGAATACGGGATATATTGAGCTTAATGATGGA

[0423] CGTAAACTTTGCCTCGAGTGTCTGGACTCAGCGGTCATGGACACAAGTCAATGTCAACCTTTGTAC

[0424] TTGCAGATACAAGATTTCTATGAAGGGCTTAACTGTACGGCTCTGTGATCATATTCCCACCCCAAT

[0425] TTCCCTTTGATCGCTTTCTTATAGTACTAACAGTTTGTTCTTCTGAAAGACACAGTCCTCTTGTCT

[0426] CTGGCATGTGATAGTGACCCTAAGAGGCACACAATGAAAAACTCAGGAGAAGCAAAATGTGATGTA

[0427] TATTCAAGTAATTACTAAGTAATGATTGTATTTTTTTACTCACATTCTTTTCACCTTCTCTGGCTT

[0428] CGTTAAGTGCTTGCCTCTCAACCAAGAGCAGTGGAACTTCCTGCTCTACCTTCAAGTTAACTCGTC

[0429] AATGCGAAGTTACTGCCATTCTCATCTTATTTGGGCTCCCTAGGCTACTGACTGGTTCGATTCTAG

[0430] CTCACGAGATGATGCACGCGTGGATGCGGCTGAAAGGATTCCGGACACTGAGCCAAGACATTGAAG

[0431] AAGGTATATGTCAAGTGATGGCTCATAAGTGGATAGAAGCCGAGTTAGCTGCTGGTTCAAGAAACA

[0432] GCAGTGCTGCATCATCATCATCATCGTCATCATCTTCTTCTTCTACTAGAGGACTGAAGAAGGGAC

[0433] CAAAATCGCAGTACGAGAGGAAGCTTGGCGAGTTTTTCAAGCACCAGATCGAGTCTGATGCGTCTC

[0434] CGGTTTATGGAGACGGGTTCAGGGCAGGGCGGCTAGCGGTTAACAAGTATGGTTTGCGAAAAACGC

[0435] TTGAGCATATACAGATGACCGGTAGATTCCCGGTTTAA darl ( -857bp between PS3 and PS4 ) ( SEQ ID NO : 79 ) :

[0436] ATGGGGTGGCTTACTAAAATCCTTAAAGGCTCAAGCCATAAATTCTCGGATGGACAAGTTAACAGA

[0437] AGATATAGAGATGATAGGAACTTGGAAAGTTCTCGTCTTTCTGCGGATGGATCTGATTTTGACAAA

[0438] GAAGAGATTGAATGTGCTATTGCACTCTCTCTTTCAGAACAAGAACATGTAATTCCACAAGATGAC

[0439] AAAGGAAAACAAGTAATCGAATACAAATCTGAAACCGAAGAAGATGAGGATGAGGATGAGGACGAA

[0440] GATGAAGAATACACGAGAGCTCAGCTAGAAGCAGCGGAAGAAGAGGAAAGACGGGCAGCTCAAGCT

[0441] CAAATCGAGGAAGAAGAGAAACGGCGAGCTGAAGCTGAATTAGAAGAAATAGAGAAACAGCTTGCG

[0442] AAAGCTCGACTAGAAGAGGAAGAAGTTAGGCGTACCAAAGCGCAATTGGAGGAAGACGAGCAACTA

[0443] GCTAAGGCCATTCAAGAAAGTATGGTTGTGGGATCTCCTCCTCGGTATGATTCTGGAAGTATGTTT

[0444] CAACCATTCCCTTTCCTTAATCCATCTAGTCACAGGGGTTGCCTAGCTGAGATTGGACATGGAAGG

[0445] TTTCTGAGTTGCATGGGTGGCGTTTGGCATCCTGAATGTTTCTGCTGCCATGCTTGTGATAAGCCC AT C AT AGAT T AT GAGT T C T C AAT GT C CGGG AAT TGGGGCAGGC T AC AAAT T GAT AGAC AT GAT C AC

[0446] AGAGCCTTGCAGGCTGATACGTCGTTGTGAAGTCACTGCAATTCTCATTCTATATGGACTTCCTCG

[0447] TTTGTTAACTGGATCAATCCTTGCTCATGAGATGATGCATGCCTGGCTTCGACTAAATGGtaaGTA

[0448] TCCGAATCTTAGGCCAGAAGTTGAAGAAGGAATATGTCAGGTTTTGGCTCACATGTGGTTGGAATC

[0449] TGAGACTTATGCTGGCTCTACATTAGTAGATATCGCATCTTCTTCATCATCATCATCATCGGCTGT

[0450] GGTCACTGCATCGTCAAAGAAAGGTGAGAGGTCTGACTTTGAGAAGAAACTTGGTGAGTTTTTCAA

[0451] GCACCAAATAGAGTCGGATTCTTCGTCGGCATATGGGGATGGGTTCAGGCAAGGTAACCAAGCAGT

[0452] TCTTACGCACGGTCTGAGGCGAACCCTCGACCATATTCGCTTGACCGGTACATTTCCTTAA

[0453] ( DA1DAR1 J -3 double mutant protein sequences :

[0454] DAI ( SEQ ID NO : 78 ) :

[0455] MGWFNKI FKGSNQRLRVGNEHNHNGYYENYPNAHASHDEPSADTDADRDEPQTQESSTSEDTSNDQ

[0456] ENEDIDRAIALSLLEENQGQKNTGGKYVMVDEDEQLARAIQESMIVGNSPRHKSGSTYDHGNAYGT

[0457] ADLFGNGHMHGGGNVYANGDIYYPRPVAFSMDFRICAGCNMEIGHGRFLNCLNSLWHPECFRCYGC

[0458] SHPI SEYEFSTSGNYPFHKACYRERYHPKCDVCNHFIPTNPAGLIEYRAHPFWIQKYCPSHEHDST

[0459] PRCCSCERMEPRNTGYIELNDGRKLCLECLDSAVMDTSQCQPLYLQIQDFYEGLNCTAL

[0460] DARI ( SEQ ID NO : 80 ) :

[0461] MGWLTKILKGSSHKFSDGQVNRRYRDDRNLESSRLSADGSDFDKEEIECAIALSLSEQEHVIPQDD

[0462] KGKQVIEYKSETEEDEDEDEDEDEEYTRAQLEAAEEEERRAAQAQIEEEEKRRAEAELEEIEKQLA

[0463] KARLEEEEVRRTKAQLEEDEQLAKAIQESMVVGSPPRYDSGSMFQPFPFLNPSSHRGCLAEIGHGR FLSCMGGVWHPECFCCHACDKPI IDYEFSMSGN upl3-l nucleic acid sequence ( -782bp between PS2 and PS 4 ) ( SEQ ID

[0464] NO : 25 ) :

[0465] ATGGAAACTCGGAGCCGCAAGCGGGCGGAGGCGACCTCAACTGCCCCATCTTCTTCTTCTTCTTCA

[0466] CCTCCTCCTCCCTCAGGCCCCACCACCCGCAGCAAACGCGCTCGTCTTTCCTCTTCCTCTTCCTCC

[0467] TCTGTTGCCGCTACCACCACTGCACCTTCCTCTTCCACCCGCTCTCGCTCTTCTCGCTCCGCCGCC

[0468] GCCGCTGCTACTCCCATGGACACCTCCACCGAGTCTTCTGGATTTCGCCGAGGCGGACGAGGTAAC

[0469] AGGGGTAACGACAACAGTAATTCTGACAAGGGCAAGGAGAAGGAGCACGACGTTAGGATTAGGGAT

[0470] AGAGAAAGAGAAAGAGACCGAGCCAGAGAACAACTCAACATGGATGCTGCCGCTGCTGCTGCCGCC

[0471] GCTGACGACGACGACGACAATGACAGCGAGGATGGCAACGGGGGTTTCATGCATCCCAACATGAGC

[0472] TCCGCCAGCAGTGCTTTACAAGGTTTGCTGAGGAAGCTTGGAGCTGGCCTCGATGACTTGCTTCCT

[0473] TCCTCTGGTATCGGCTCTGCTTCTTCTTCCCACTTAAATGGGAGGATGAAGAAGATACTCTCTGGC

[0474] TTGCGCTCCGAAGGAGAAGAGGGAAAGCAGGTCGAGGCTTTAACCCAGCTATGCGAGATGCTATCC

[0475] ATTGGGACTGAAGACTCATTAAGCACCTTCTCTGTTGATTCCTTCGTCCCTGTTCTCGTTGGTCTA

[0476] CTAAACCATGAAAGCAATCCCGACATAATGCTTCTTGCGGCCAGGGCTCTTACCCATCTATGTGAT

[0477] GTTTTGCCGTCACACGGGGTTAATCCGATTACTTTCCACCTGTGCGAGCGGCTCACCTCTTGGATT

[0478] CAGGACATTGCTTCTTCTCGGCATTAGTAGCATTCTTAAGGATATTCTGTCGGGTCCTGGTGTCTC

[0479] TGCTAATGCATCTGTATCTCCAGCACTGAGCAGGCCCGCAGATCAGATCTTTGAGATAGTCAACCT

[0480] AGCGAATGAGCTCCTCCCTCCATTGCCAGAAGGAGTTATCTCCCTTCCTACTAGCACAAACACTTT

[0481] GGTGAAAGGTTCAGGCCAAAAGAAATCTTCTCCAAGTACTTCAGGAAAACAAGACGATTCACCAAA

[0482] AGTTTCACCTAGAGAAAAATTACTTGCTGATCAACCTGAACTTCTGCAGAAATTTGGACTGGATCT

[0483] TCTTCCAGTTTTAGTGCAGATTTATGGTTCTAGTGTCAATGGTACTATTCGCCATAAATGTCTGTC

[0484] AGTTATCGGAAAGTTGATGTATTTCAGCACTTCAGAAATGATTCAATCTCTAATTGGTGACACAAA

[0485] TATATCGAGCTTCTTGGCTGGTGTATTGGCATGGAAAGATCCACAAGTCTTGGTTCCTGCTCTACA

[0486] AGTTGCAGAAATTCTGATGGAAAAGCTTCCTGAAACATTCTCGAAAGTGTTTGTGAGGGAAGGGGT

[0487] GGTTCATGCTGTAGATCAACTTGTCTTGGTTGGTAAACCTATTTCTCATGCTTCACCTACTGATAA

[0488] GGAAAATGACTGTGTGCCAGGATCTGCACGATCTAGGCGATATAGACGGCGAAGTAGTAATGCCAG

[0489] TTCTGATGGAAATCAGTCAGAAGAGCTTAAGAATTCTGCGTCGGTTAACGTAGGTGCAAACCATAA

[0490] TTCCCTAGATTCTCCTACCTCTAGCTTCTTGCTAAAGGAAACAGTTAGCTCCTGTGCCAAAGCATT CAAAGACAAGTACTTCCCGTCTGATGGCGGGGATCTCGATGTTGGAGTTACAGATGATCTCTTACA TCTAAAGAATCTTTGCACGAAGCTAACTGCTAGTATAGATGAGCATAAAGTGAAAGGAAAGGGAAA ATCCAAAGCCTCTGGGCCATGCCTTGGCGATTTTTCTGCTAGCAAGGAAGAATACTTGATTGGTGT CATTTCTGAGATACTTGGCGAGCTTAGCAAAGGGGATGGAGTCTCAACTTTTGAGTTTATTGGCAG TGGTGTGGTTGCAGCTTTGCTTGACTATTTCTCTTGTGGGTACTTTTCCAAAGAGAAGATCTCCGA GGTTAATTTGCCCAAACTTCGCCAAGATGGGCTCAGAAGATTCAAAGCTTTTCTAGAAGTCGCACT TCCGTCTGATGGTAATGAGGGAAAGATCCCTCCTATGACAGTTTTGATTCAGAAACTTCAAGATGC TTTATCTTCACTGGAACGCTTTCCCGTCGTCCTTAGTCATCCCTCAAGGTCATTAGGTGGAAGTGC TCGTCTCTCATCTGGATTGAGTGCTTTGGCACATCCTTTAAAGTTGCGATTATGCCGTGCACCTGG AGAGAAGACACTACGTGATTACTCCTCCAATATTGTTCTTATAGATCCACTGGCAAGCATTGCAGC GGTAGAAGAATTTCTTTGGCCCCGAGTTCAACGGAGTGAATCTGGTATGAAGCATGCAGCGCCTGC TGGAAATACAGAGCCAGGCACGTTACCTAGCGGTGCTGGTGTTTCATCACCATCCTCGTCAACTCC GGCTTCCACCACACGTCGTCATTCTTCTAGATCTAGATCAGCAATTAACATAGGTGATACCTCAAA GAAAGAAGCTGTGCATGAGAAAGGTACCAGCTCATCTAAAGGAAAAGGTAAAGGCGTTATGAAGCC CGTTCCAGCTGATAAGGGGCCTCAAACAAGGAGCAGTGCTCAAAGAAGAGCTGTCCTTGACAAAGA TACACATATGAAACCAGCTAGCGGAGACTCCAGCTCCGAGGATGAGGAATTGGAAATAGCCCCTGT C GAG AT T GAT GAT GCC T T GGT GAT T G AAGAGGAT GAG AT GT CT GAT GAT GAAGAT GAT G AT AAT GA CGATGTGTTGGATGACAGTCTTCCCATGTGCACGCCTGATAAAGTGCATGATGTAAAATTGGGGGA CGCAGTGGATGATGACGGTGGTGCTGGTCTGGCCTCTAGCGGGCGACAGATGAATCCAGCTTCAGG TGGCACTAGTGGAGCAGCAGCAGCGAGGGGATCTGATTCTATTGATGCTGGCATTGGGAATTCTTA CGGTTCTAGGGGTGCACTCTCCTTTGCTGCTGCGGCAATGGCTGGGCTTGGAGCTGCCAGTGGTAG AGGTATCAGGGGAAGTAGAGACCTGCATGGGCGTACCCTATATCGAAGTTCCGACGAGCCCTCTAA GTTATTATTTACTGCAGGAGGAAAGCAACTTAGTAGGCATTTGACGATATATCAGGCTGTGCAGCG ACAACTTATGCTAGATGAAGACGATGATGAAAGGTTCGGTGGCAGCGATTTCATGTCAAGTGATGG AAGCAGATTCAATGATATTTACACCATCATGTACCAGAGGCCGGAGAGTCAAGCGAATAGGTTGTC TGTTGGTGCAGCAAGTTCTACCACACTATCAAAATCCACTAAATCTGCTACTGCCAATTCTAGCGT AGAATCTCAGTCATATAAGGCATCTCTTTTGGATAGTATCTTACAAGGGGAGCTTCCGTGCGATCT TGAGAAGTCAAATTCTACTTATAATGTTCTGGCGCTGTTGCGTGTATTAGAGGGGTTAAATCAGCT TGGCCCTCGGTTGAGAGCCCAAACTGTTTCTGATCGTTTCGCAGAGGGTAAAATTACAAGTCTAGA TGATCTGAAGACAACTGCTGCTGCTAAGATTTCTCATGAAGAATTCATCAATAGCAAACTTACACC CAAATTAGCTCGACAGATCCAGGATGCGCTTGCTTTGTGCAGTGGTAGTCTTCCCTCTTGGTGCTA CCAGTTGACTAGAGCATGCCCGTTTTTGTTTCCGTTTCAGACCCGGAGACAGTATTTCTATTCAAC TGCTTTTGGGTTGTCCCGCGCATTGAACCGCTTGCAGCAGCAGCAAGGTGCTGACGGCAGTGGGTC TACAAATGAACGAGAGATGAGAATAGGGAGGTTGCAGCGCCAGAAAGTCCGTGTATCCCGAAATAG AATATTAGATTCTGCTGCGAAAGTTATGGAGATGTATTCTAGCCAAAAAGCTGTGCTTGAAGTAGA ATATTTTGGTGAAGTTGGTACTGGTCTAGGCCCTACACTTGAGTTTTACACACTCCTAAGCCATGA TCTACAAAAGGTTGCGCTTGGGATGTGGAGATCAAATTCTGGTGACATGGTATCTATGCAAATTGA TAGAGGTGAGATTGAAGACGGGAAATCATCCGCAGCTAGGGACAGAGATATAGTTCAGGCACCGCT TGGGTTGTTTCCTCGACCTTGGCCCTCTACAGCTGACGTATCTGAAGGTAGTCGGTTTCATAAAGT CATTGAATATTTCCGGCTTTTAGGGCGTGTGATGGCAAAAGCACTTCAAGATGGACGGCTACTGGA CGTCCCATTGAGTACAGCGTTTTATAAGCTTATTCTTGGTCAAGAGCTTGATTTGCATGATATCAT ATTATTTGATACCGAGCTTGGCAAGACTTTGCAAGAGTTTCGTGTTCTTGTTGGCCGCAAGCACTA TCTGGAGGCAGTAGGCGGTGACAATTGCAGCGCTGGTTCTGATTTATGTTTACGTGGATCCCGTAT AGAAGATCTCTGCTTGGACTTCACGCTTCCTGGCTATCCTGAATACATTCTGAGACCAGGAGATGA AATTGTTGATATGAATAGTCTTGAAGAGTATATATCCCTTGTCGTTGATGCTACTGTCAAGAGAGG AGTTACCCGGCAGATTGAAGCCTTTAGATCTGGATTCAATCAGGTGTTTGACGTAACATCTCTACA AATATTCACCCCTTCCGAGCTGGACTATTTGCTGTGTGGTCGTAGAGAGTTGTGGGAGGCGGAGAC TCTTGCTGAACATATCAAGTTTGATCACGGTTATACTGCTAAAAGTCCGGCAATCATTAATTTACT GGAGATCATGGGAGAACTTACAGCGGATCAGCAGAGGGCTTTCTGCCAATTTGTAACTGGAGCTCC TAGGCTTCCTCCTGGTGGGCTAGCTGTTTTGAATCCAAAGCTGACGATTGTGAGAAAGCATTCATC AACCTCAAGTGCGGCAGCGAACGGAACAGGGGCTTCGGAGACGGCAGACGATGATCTTCCCAGCGT AATGACTTGCGCCAACTATCTTAAGCTCCCTCCTTATTCGACAAAGGAAATCATGTACAAGAAACT GCTCTACGCCATCAACGAAGGGCAAGGATCGTTCGACCTGTCATAA

[0491] UPL3-1 protein sequence ( SEQ ID NO : 26 ) :

[0492] METRSRKRAEATSTAPSSSSSSPPPPSGPTTRSKRARLSSSSSSSVAATTTAPSSSTRSRSSRSAA AAATPMDTSTESSGFRRGGRGNRGNDNSNSDKGKEKEHDVRIRDRERERDRAREQLNMDAAAAAAA ADDDDDNDSEDGNGGFMHPNMSSASSALQGLLRKLGAGLDDLLPSSGIGSASSSHLNGRMKKILSG LRSEGEEGKQVEALTQLCEMLSIGTEDSLSTFSVDSFVPVLVGLLNHESNPDIMLLAARALTHLCD VLPS . upl3-2 nucleic acid sequence ( -1105bp between PSI and PS4 ) ( SEQ ID

[0493] NO : 27 ) :

[0494] ATGGAAACTCGGAGCCGCAAGCGGGCGGAGGCGACCTCAACTGCCCCATCTTCTTCTTCTTCTTCA CCTCCTCCTCCCTCAGGCCCCACCACCCGCAGCAAACGCGCTCGTCTTTCCTCTTCCTCTTCCTCC TCTGTTGCCGCTACCACCACTGCACCTTCCTCTTCCACCCGCTCTCGCTCTTCTCGCTCCGCCGCC GCCGCTGCTACTCCCATGGACACCTCCACCGAGTCTTCTGGATTTCGCCGAGGCGGACGAGGTAAC AGGGGTAACGACAACAGTAATTCTGACAAGGGCAAGGAGAAGGAGCACGACGTTAGGATTAGGGAT AGAGAAAGAGAAAGAGACCGAGCCAGAGAACAACTCAACATGGATGCTGCCGCTGCTGCTGCCGCC GCTGACGACGACGACGACAATGACAGCGAGGATGGCAACGGGGGTTTCATGCATCCCAACATGAGC TCCGCCAGCAGTGCTTTTACACGGGGTTAATCCGATTACTTTCCACCTGTGCGAGCGGCTCACCTC TTGGATTCAGGACATTGCTTCTTCTCGGCATTAGTAGCATTCTTAAGGATATTCTGTCGGGTCCTG GTGTCTCTGCTAATGCATCTGTATCTCCAGCACTGAGCAGGCCCGCAGATCAGATCTTTGAGATAG TCAACCTAGCGAATGAGCTCCTCCCTCCATTGCCAGAAGGAGTTATCTCCCTTCCTACTAGCACAA ACACTTTGGTGAAAGGTTCAGGCCAAAAGAAATCTTCTCCAAGTACTTCAGGAAAACAAGACGATT CACCAAAAGTTTCACCTAGAGAAAAATTACTTGCTGATCAACCTGAACTTCTGCAGAAATTTGGAC TGGATCTTCTTCCAGTTTTAGTGCAGATTTATGGTTCTAGTGTCAATGGTACTATTCGCCATAAAT GTCTGTCAGTTATCGGAAAGTTGATGTATTTCAGCACTTCAGAAATGATTCAATCTCTAATTGGTG ACACAAATATATCGAGCTTCTTGGCTGGTGTATTGGCATGGAAAGATCCACAAGTCTTGGTTCCTG CTCTACAAGTTGCAGAAATTCTGATGGAAAAGCTTCCTGAAACATTCTCGAAAGTGTTTGTGAGGG AAGGGGTGGTTCATGCTGTAGATCAACTTGTCTTGGTTGGTAAACCTATTTCTCATGCTTCACCTA CTGATAAGGAAAATGACTGTGTGCCAGGATCTGCACGATCTAGGCGATATAGACGGCGAAGTAGTA ATGCCAGTTCTGATGGAAATCAGTCAGAAGAGCTTAAGAATTCTGCGTCGGTTAACGTAGGTGCAA ACCATAATTCCCTAGATTCTCCTACCTCTAGCTTCTTGCTAAAGGAAACAGTTAGCTCCTGTGCCA AAGCATTCAAAGACAAGTACTTCCCGTCTGATGGCGGGGATCTCGATGTTGGAGTTACAGATGATC TCTTACATCTAAAGAATCTTTGCACGAAGCTAACTGCTAGTATAGATGAGCATAAAGTGAAAGGAA AGGGAAAATCCAAAGCCTCTGGGCCATGCCTTGGCGATTTTTCTGCTAGCAAGGAAGAATACTTGA TTGGTGTCATTTCTGAGATACTTGGCGAGCTTAGCAAAGGGGATGGAGTCTCAACTTTTGAGTTTA TTGGCAGTGGTGTGGTTGCAGCTTTGCTTGACTATTTCTCTTGTGGGTACTTTTCCAAAGAGAAGA TCTCCGAGGTTAATTTGCCCAAACTTCGCCAAGATGGGCTCAGAAGATTCAAAGCTTTTCTAGAAG TCGCACTTCCGTCTGATGGTAATGAGGGAAAGATCCCTCCTATGACAGTTTTGATTCAGAAACTTC AAGATGCTTTATCTTCACTGGAACGCTTTCCCGTCGTCCTTAGTCATCCCTCAAGGTCATTAGGTG GAAGTGCTCGTCTCTCATCTGGATTGAGTGCTTTGGCACATCCTTTAAAGTTGCGATTATGCCGTG CACCTGGAGAGAAGACACTACGTGATTACTCCTCCAATATTGTTCTTATAGATCCACTGGCAAGCA TTGCAGCGGTAGAAGAATTTCTTTGGCCCCGAGTTCAACGGAGTGAATCTGGTATGAAGCATGCAG CGCCTGCTGGAAATACAGAGCCAGGCACGTTACCTAGCGGTGCTGGTGTTTCATCACCATCCTCGT CAACTCCGGCTTCCACCACACGTCGTCATTCTTCTAGATCTAGATCAGCAATTAACATAGGTGATA CCTCAAAGAAAGAAGCTGTGCATGAGAAAGGTACCAGCTCATCTAAAGGAAAAGGTAAAGGCGTTA TGAAGCCCGTTCCAGCTGATAAGGGGCCTCAAACAAGGAGCAGTGCTCAAAGAAGAGCTGTCCTTG ACAAAGATACACATATGAAACCAGCTAGCGGAGACTCCAGCTCCGAGGATGAGGAATTGGAAATAG CCCCTGTCGACATTGATGATGCCTTGGTGATTGAAGAGGATGACATGTCTGATGATGAAGATGATG ATAATGACGATGTGTTGGATGACAGTCTTCCCATGTGCACGCCTGATAAAGTGCATGATGTAAAAT TGGGGGACGCAGTGGATGATGACGGTGGTGCTGGTCTGGCCTCTAGCGGGCGACAGATGAATCCAG

[0495] CTTCAGGTGGCACTAGTGGAGCAGCAGCAGCGAGGGGATCTGATTCTATTGATGCTGGCATTGGGA

[0496] ATTCTTACGGTTCTAGGGGTGCACTCTCCTTTGCTGCTGCGGCAATGGCTGGGCTTGGAGCTGCCA

[0497] GTGGTAGAGGTATCAGGGGAAGTAGAGACCTGCATGGGCGTACCCTATATCGAAGTTCCGACGAGC

[0498] CCTCTAAGTTATTATTTACTGCAGGAGGAAAGCAACTTAGTAGGCATTTGACGATATATCAGGCTG

[0499] TGCAGCGACAACTTATGCTAGATGAAGACGATGATGAAAGGTTCGGTGGCAGCGATTTCATGTCAA

[0500] GTGATGGAAGCAGATTCAATGATATTTACACCATCATGTACCAGAGGCCGGAGAGTCAAGCGAATA

[0501] GGTTGTCTGTTGGTGCAGCAAGTTCTACCACACTATCAAAATCCACTAAATCTGCTACTGCCAATT

[0502] CTAGCGTAGAATCTCAGTCATATAAGGCATCTCTTTTGGATAGTATCTTACAAGGGGAGCTTCCGT

[0503] GCGATCTTGAGAAGTCAAATTCTACTTATAATGTTCTGGCGCTGTTGCGTGTATTAGAGGGGTTAA

[0504] ATCAGCTTGGCCCTCGGTTGAGAGCCCAAACTGTTTCTGATCGTTTCGCAGAGGGTAAAATTACAA

[0505] GTCTAGATGATCTGAAGACAACTGCTGCTGCTAAGATTTCTCATGAAGAATTCATCAATAGCAAAC

[0506] TTACACCCAAATTAGCTCGACAGATCCAGGATGCGCTTGCTTTGTGCAGTGGTAGTCTTCCCTCTT

[0507] GGTGCTACCAGTTGACTAGAGCATGCCCGTTTTTGTTTCCGTTTCAGACCCGGAGACAGTATTTCT

[0508] ATTCAACTGCTTTTGGGTTGTCCCGCGCATTGAACCGCTTGCAGCAGCAGCAAGGTGCTGACGGCA

[0509] GTGGGTCTACAAATGAACGAGAGATGAGAATAGGGAGGTTGCAGCGCCAGAAAGTCCGTGTATCCC

[0510] GAAATAGAATATTAGATTCTGCTGCGAAAGTTATGGAGATGTATTCTAGCCAAAAAGCTGTGCTTG

[0511] AAGTAGAATATTTTGGTGAAGTTGGTACTGGTCTAGGCCCTACACTTGAGTTTTACACACTCCTAA

[0512] GCCATGATCTACAAAAGGTTGCGCTTGGGATGTGGAGATCAAATTCTGGTGACATGGTATCTATGC

[0513] AAATTGATAGAGGTGAGATTGAAGACGGGAAATCATCCGCAGCTAGGGACAGAGATATAGTTCAGG

[0514] CACCGCTTGGGTTGTTTCCTCGACCTTGGCCCTCTACAGCTGACGTATCTGAAGGTAGTCGGTTTC

[0515] ATAAAGTCATTGAATATTTCCGGCTTTTAGGGCGTGTGATGGCAAAAGCACTTCAAGATGGACGGC

[0516] TACTGGACGTCCCATTGAGTACAGCGTTTTATAAGCTTATTCTTGGTCAAGAGCTTGATTTGCATG

[0517] ATATCATATTATTTGATACCGAGCTTGGCAAGACTTTGCAAGAGTTTCGTGTTCTTGTTGGCCGCA

[0518] AGCACTATCTGGAGGCAGTAGGCGGTGACAATTGCAGCGCTGGTTCTGATTTATGTTTACGTGGAT

[0519] CCCGTATAGAAGATCTCTGCTTGGACTTCACGCTTCCTGGCTATCCTGAATACATTCTGAGACCAG

[0520] GAGATGAAATTGTTGATATGAATAGTCTTGAAGAGTATATATCCCTTGTCGTTGATGCTACTGTCA

[0521] AGAGAGGAGTTACCCGGCAGATTGAAGCCTTTAGATCTGGATTCAATCAGGTGTTTGACGTAACAT

[0522] CTCTACAAATATTCACCCCTTCCGAGCTGGACTATTTGCTGTGTGGTCGTAGAGAGTTGTGGGAGG

[0523] CGGAGACTCTTGCTGAACATATCAAGTTTGATCACGGTTATACTGCTAAAAGTCCGGCAATCATTA

[0524] ATTTACTGGAGATCATGGGAGAACTTACAGCGGATCAGCAGAGGGCTTTCTGCCAATTTGTAACTG

[0525] GAGCTCCTAGGCTTCCTCCTGGTGGGCTAGCTGTTTTGAATCCAAAGCTGACGATTGTGAGAAAGC

[0526] ATTCATCAACCTCAAGTGCGGCAGCGAACGGAACAGGGGCTTCGGAGACGGCAGACGATGATCTTC

[0527] CCAGCGTAATGACTTGCGCCAACTATCTTAAGCTCCCTCCTTATTCGACAAAGGAAATCATGTACA AGAAACTGCTCTACGCCATCAACGAAGGGCAAGGATCGTTCGACCTGTCATAA

[0528] UPL3-2 protein sequence ( SEQ ID NO : 28 ) :

[0529] METRSRKRAEATSTAPSSSSSSPPPPSGPTTRSKRARLSSSSSSSVAATTTAPSSSTRSRSSRSAA

[0530] AAATPMDTSTESSGFRRGGRGNRGNDNSNSDKGKEKEHDVRIRDRERERDRAREQLNMDAAAAAAA ADDDDDNDSE DGNGGFMH PNMS SAS SA . upl3-3 nucleic acid sequence ( inversion of 326 bp between PSI and

[0531] PS2 ) ( SEQ ID NO : 29 ) :

[0532] ATGGAAACTCGGAGCCGCAAGCGGGCGGAGGCGACCTCAACTGCCCCATCTTCTTCTTCTTCTTCA

[0533] CCTCCTCCTCCCTCAGGCCCCACCACCCGCAGCAAACGCGCTCGTCTTTCCTCTTCCTCTTCCTCC

[0534] TCTGTTGCCGCTACCACCACTGCACCTTCCTCTTCCACCCGCTCTCGCTCTTCTCGCTCCGCCGCC

[0535] GCCGCTGCTACTCCCATGGACACCTCCACCGAGTCTTCTGGATTTCGCCGAGGCGGACGAGGTAAC

[0536] AGGGGTAACGACAACAGTAATTCTGACAAGGGCAAGGAGAAGGAGCACGACGTTAGGATTAGGGAT

[0537] AGAGAAAGAGAAAGAGACCGAGCCAGAGAACAACTCAACATGGATGCTGCCGCTGCTGCTGCCGCC

[0538] GCTGACGACGACGACGACAATGACAGCGAGGATGGCAACGGGGGTTTCATGCATCCCAACATGAGC

[0539] TCCGCCAGCAGTGCTTTAAGACGGCAAAACATCACATAGATGGGTAAGAGCCCTGGCCGCAAGAAG CATTATGTCGGGATTGCTTTCATGGTTTAGTAGACCAACGAGAACAGGGACGAAGGAATCAACAGA

[0540] GAAGGTGCTTAATGAGTCTTCAGTCCCAATGGATAGCATCTCGCATAGCTGGGTTAAAGCCTCGAC

[0541] CTGCTTTCCCTCTTCTCCTTCGGAGCGCAAGCCAGAGAGTATCTTCTTCATCCTCCCATTTAAGTG

[0542] GGAAGAAGAAGCAGAGCCGATACCAGAGGAAGGAAGCAAGTCATCGAGGCCAGCTCCAAGCTTCCT

[0543] CAGCAAACCTTGTTCTTGTGCCGCCGTTGTACATTACGGCGCTGTTTCATGCTTTGTCGCCAGATT

[0544] GCTAACCATCGAGTACATGGACTTGGCCGAGCAGTCTCTGCAAGCTCTCAAAAAGATATCTCAGGA

[0545] GCACCCAACTGCCTGTTTGCGAGCTGGCGCTCTGATGGCAGTGCTCTCATATCTTGATTTCTTCTC

[0546] CACTGGAGTCCAGCGTGTAGCACTATCTACTGCTGCAAATATGTGCAAGAAATTACCTTCTGATGC

[0547] ATCTGATTATGTTATGGAAGCTGTACCATTACTGACAAACCTACTTCAGTATCATGATTCGAAGGT

[0548] TTTGGAATATGCTTCTATATGTCTGACTCGGATTGCTGAAGCATTTGCTTCGCACCCTGAGAAATT

[0549] GGATGAATTATGCAACCATGGCCTTGTGACTCAAGCTGCGTCTCTTATTTCCACTACCAACTCAGG

[0550] AGGTGGGCAAGCATCTCTGGGCGTTTCAACATACACGGGGTTAATCCGATTACTTTCCACCTGTGC

[0551] GAGCGGCTCACCTCTTGGATTCAGGACATTGCTTCTTCTCGGCATTAGTAGCATTCTTAAGGATAT

[0552] TCTGTCGGGTCCTGGTGTCTCTGCTAATGCATCTGTATCTCCAGCACTGAGCAGGCCCGCAGATCA

[0553] GATCTTTGAGATAGTCAACCTAGCGAATGAGCTCCTCCCTCCATTGCCAGAAGGAGTTATCTCCCT

[0554] TCCTACTAGCACAAACACTTTGGTGAAAGGTTCAGGCCAAAAGAAATCTTCTCCAAGTACTTCAGG

[0555] AAAACAAGACGATTCACCAAAAGTTTCACCTAGAGAAAAATTACTTGCTGATCAACCTGAACTTCT

[0556] GCAGAAATTTGGACTGGATCTTCTTCCAGTTTTAGTGCAGATTTATGGTTCTAGTGTCAATGGTAC

[0557] TATTCGCCATAAATGTCTGTCAGTTATCGGAAAGTTGATGTATTTCAGCACTTCAGAAATGATTCA

[0558] ATCTCTAATTGGTGACACAAATATATCGAGCTTCTTGGCTGGTGTATTGGCATGGAAAGATCCACA

[0559] AGTCTTGGTTCCTGCTCTACAAGTTGCAGAAATTCTGATGGAAAAGCTTCCTGAAACATTCTCGAA

[0560] AGTGTTTGTGAGGGAAGGGGTGGTTCATGCTGTAGATCAACTTGTCTTGGTTGGTAAACCTATTTC

[0561] TCATGCTTCACCTACTGATAAGGAAAATGACTGTGTGCCAGGATCTGCACGATCTAGGCGATATAG

[0562] ACGGCGAAGTAGTAATGCCAGTTCTGATGGAAATCAGTCAGAAGAGCTTAAGAATTCTGCGTCGGT

[0563] TAACGTAGGTGCAAACCATAATTCCCTAGATTCTCCTACCTCTAGCTTCTTGCTAAAGGAAACAGT

[0564] TAGCTCCTGTGCCAAAGCATTCAAAGACAAGTACTTCCCGTCTGATGGCGGGGATCTCGATGTTGG

[0565] AGTTACAGATGATCTCTTACATCTAAAGAATCTTTGCACGAAGCTAACTGCTAGTATAGATGAGCA

[0566] TAAAGTGAAAGGAAAGGGAAAATCCAAAGCCTCTGGGCCATGCCTTGGCGATTTTTCTGCTAGCAA

[0567] GGAAGAATACTTGATTGGTGTCATTTCTGAGATACTTGGCGAGCTTAGCAAAGGGGATGGAGTCTC

[0568] AACTTTTGAGTTTATTGGCAGTGGTGTGGTTGCAGCTTTGCTTGACTATTTCTCTTGTGGGTACTT

[0569] TTCCAAAGAGAAGATCTCCGAGGTTAATTTGCCCAAACTTCGCCAAGATGGGCTCAGAAGATTCAA

[0570] AGCTTTTCTAGAAGTCGCACTTCCGTCTGATGGTAATGAGGGAAAGATCCCTCCTATGACAGTTTT

[0571] GATTCAGAAACTTCAAGATGCTTTATCTTCACTGGAACGCTTTCCCGTCGTCCTTAGTCATCCCTC

[0572] AAGGTCATTAGGTGGAAGTGCTCGTCTCTCATCTGGATTGAGTGCTTTGGCACATCCTTTAAAGTT

[0573] GCGATTATGCCGTGCACCTGGAGAGAAGACACTACGTGATTACTCCTCCAATATTGTTCTTATAGA

[0574] TCCACTGGCAAGCATTGCAGCGGTAGAAGAATTTCTTTGGCCCCGAGTTCAACGGAGTGAATCTGG

[0575] TATGAAGCATGCAGCGCCTGCTGGAAATACAGAGCCAGGCACGTTACCTAGCGGTGCTGGTGTTTC

[0576] ATCACCATCCTCGTCAACTCCGGCTTCCACCACACGTCGTCATTCTTCTAGATCTAGATCAGCAAT

[0577] TAACATAGGTGATACCTCAAAGAAAGAAGCTGTGCATGAGAAAGGTACCAGCTCATCTAAAGGAAA

[0578] AGGTAAAGGCGTTATGAAGCCCGTTCCAGCTGATAAGGGGCCTCAAACAAGGAGCAGTGCTCAAAG

[0579] AAGAGCTGTCCTTGACAAAGATACACATATGAAACCAGCTAGCGGAGACTCCAGCTCCGAGGATGA

[0580] GGAATTGGAAATAGCCCCTGTCGACATTGATGATGCCTTGGTGATTGAAGAGGATGACATGTCTGA

[0581] TGATGAAGATGATGATAATGACGATGTGTTGGATGACAGTCTTCCCATGTGCACGCCTGATAAAGT

[0582] GCATGATGTAAAATTGGGGGACGCAGTGGATGATGACGGTGGTGCTGGTCTGGCCTCTAGCGGGCG

[0583] ACAGATGAATCCAGCTTCAGGTGGCACTAGTGGAGCAGCAGCAGCGAGGGGATCTGATTCTATTGA

[0584] TGCTGGCATTGGGAATTCTTACGGTTCTAGGGGTGCACTCTCCTTTGCTGCTGCGGCAATGGCTGG

[0585] GCTTGGAGCTGCCAGTGGTAGAGGTATCAGGGGAAGTAGAGACCTGCATGGGCGTACCCTATATCG

[0586] AAGTTCCGACGAGCCCTCTAAGTTATTATTTACTGCAGGAGGAAAGCAACTTAGTAGGCATTTGAC

[0587] GATATATCAGGCTGTGCAGCGACAACTTATGCTAGATGAAGACGATGATGAAAGGTTCGGTGGCAG

[0588] CGATTTCATGTCAAGTGATGGAAGCAGATTCAATGATATTTACACCATCATGTACCAGAGGCCGGA

[0589] GAGTCAAGCGAATAGGTTGTCTGTTGGTGCAGCAAGTTCTACCACACTATCAAAATCCACTAAATC TGCTACTGCCAATTCTAGCGTAGAATCTCAGTCATATAAGGCATCTCTTTTGGATAGTATCTTACA

[0590] AGGGGAGCTTCCGTGCGATCTTGAGAAGTCAAATTCTACTTATAATGTTCTGGCGCTGTTGCGTGT ATTAGAGGGGTTAAATCAGCTTGGCCCTCGGTTGAGAGCCCAAACTGTTTCTGATCGTTTCGCAGA GGGTAAAATTACAAGTCTAGATGATCTGAAGACAACTGCTGCTGCTAAGATTTCTCATGAAGAATT CATCAATAGCAAACTTACACCCAAATTAGCTCGACAGATCCAGGATGCGCTTGCTTTGTGCAGTGG TAGTCTTCCCTCTTGGTGCTACCAGTTGACTAGAGCATGCCCGTTTTTGTTTCCGTTTCAGACCCG GAGACAGTATTTCTATTCAACTGCTTTTGGGTTGTCCCGCGCATTGAACCGCTTGCAGCAGCAGCA AGGTGCTGACGGCAGTGGGTCTACAAATGAACGAGAGATGAGAATAGGGAGGTTGCAGCGCCAGAA AGTCCGTGTATCCCGAAATAGAATATTAGATTCTGCTGCGAAAGTTATGGAGATGTATTCTAGCCA AAAAGCTGTGCTTGAAGTAGAATATTTTGGTGAAGTTGGTACTGGTCTAGGCCCTACACTTGAGTT TTACACACTCCTAAGCCATGATCTACAAAAGGTTGCGCTTGGGATGTGGAGATCAAATTCTGGTGA CATGGTATCTATGCAAATTGATAGAGGTGAGATTGAAGACGGGAAATCATCCGCAGCTAGGGACAG AGATATAGTTCAGGCACCGCTTGGGTTGTTTCCTCGACCTTGGCCCTCTACAGCTGACGTATCTGA AGGTAGTCGGTTTCATAAAGTCATTGAATATTTCCGGCTTTTAGGGCGTGTGATGGCAAAAGCACT TCAAGATGGACGGCTACTGGACGTCCCATTGAGTACAGCGTTTTATAAGCTTATTCTTGGTCAAGA GCTTGATTTGCATGATATCATATTATTTGATACCGAGCTTGGCAAGACTTTGCAAGAGTTTCGTGT TCTTGTTGGCCGCAAGCACTATCTGGAGGCAGTAGGCGGTGACAATTGCAGCGCTGGTTCTGATTT ATGTTTACGTGGATCCCGTATAGAAGATCTCTGCTTGGACTTCACGCTTCCTGGCTATCCTGAATA CATTCTGAGACCAGGAGATGAAATTGTTGATATGAATAGTCTTGAAGAGTATATATCCCTTGTCGT TGATGCTACTGTCAAGAGAGGAGTTACCCGGCAGATTGAAGCCTTTAGATCTGGATTCAATCAGGT GTTTGACGTAACATCTCTACAAATATTCACCCCTTCCGAGCTGGACTATTTGCTGTGTGGTCGTAG AGAGTTGTGGGAGGCGGAGACTCTTGCTGAACATATCAAGTTTGATCACGGTTATACTGCTAAAAG TCCGGCAATCATTAATTTACTGGAGATCATGGGAGAACTTACAGCGGATCAGCAGAGGGCTTTCTG CCAATTTGTAACTGGAGCTCCTAGGCTTCCTCCTGGTGGGCTAGCTGTTTTGAATCCAAAGCTGAC GATTGTGAGAAAGCATTCATCAACCTCAAGTGCGGCAGCGAACGGAACAGGGGCTTCGGAGACGGC AGACGATGATCTTCCCAGCGTAATGACTTGCGCCAACTATCTTAAGCTCCCTCCTTATTCGACAAA GGAAATCATGTACAAGAAACTGCTCTACGCCATCAACGAAGGGCAAGGATCGTTCGACCTGTCATA A

[0591] UPL3-3 protein sequence ( SEQ ID NO : 30 ) :

[0592] METRSRKRAEATSTAPSSSSSSPPPPSGPTTRSKRARLSSSSSSSVAATTTAPSSSTRSRSSRSAA AAATPMDTSTESSGFRRGGRGNRGNDNSNSDKGKEKEHDVRIRDRERERDRAREQLNMDAAAAAAA ADDDDDNDSEDGNGGFMHPNMSSASSAL .

[0593] ( dal tt8fael ) -1 triple mutant nucleic acid sequences ( +A in PSA in

[0594] DAI ; stacked in tt8fael ) ( SEQ ID NO : 81 ) :

[0595] ATGGGTTGGTTCAACAAGATCTTCAAAGGCTCTAACCAAAGGCTCCGGGTGGGGAATGAGCACAAT CACAATGGCTATTACGAGAACTATCCAAATGCGCATGCTTCACACGACGAGCCTAGTGCGGATACA GATGCTGATCGTGATGAACCTCAGACGCAGGAATCATCTACATCTGAGGATACGTCGAATGACCAG GAAAATGAAGACATAGACCGTGCAATCGCGTTGTCTCTTTTAGAAGAGAATCAAGGACAGAAAAAT ACAGGCGGGAAATACGTAATGGTGGATGAAGATGAGCAGCTTGCTAGAGCCATACAAGAAAGTATG ATAGTTGGGAATTCACCGCGTCACAAAAGTGGAAGTACGTATGATCATGGGAATGCATATGGGACT GCAGATTTATTTGGGAATGGTCACATGCATGGAGGAGGAAATGTATATGCAAATGGAGATATTTAT TACCCAAGACCCGTTGCTTTTTCAATGGATTTCAGGATTTGTGCTGGCTGCAATATGGAGATTGGC CATGGAAGATTTCTGAATTGCCTTAACTCACTATGGCATCCAGAATGTTTTCGATGTTATGGCTGT AGTCACCCGATTTCTGAGTACGAGTTTTCAACATCTGGGAACTACCCTTTTCACAAGGCTTGTTAC AGGGAGAGATATCATCCAAAATGTGATGTCTGCAACCACTTTATTCCAACAAATCCTGCTGGTCTT ATTGAATATAGGGCACACCCATTTTGGATCCAGAAGTATTGCCCTTCTCACGAACACGATTCCACC CCAAGATGTTGCAGTTGTGAAAGAATGGAGCCACGGAATACGGGATATATTGAGCTTAATGATGGA CGTAAACTTTGCCTCGAGTGTCTGGACTCAGCGGTCATGGACACAAGTCAATGTCAACCTTTGTAC TTGCAGATACAAGATTTCTATGAAGGGCTTAACAATGAAGGTAGAGCAGGAAGTTCCACTGCTCTT GGTTGAGAGGCAAGCACTTAACGAAGCCAGAGAAGGTGAAAAGAATGGTCACTATCACATGCCAGA

[0596] GACAAGAGGACTGTGTCTTTCAGAAGAACAAACTGTTAGTACTATAAGAAAGCGATCAAAGGGAAA TTGGGGTGGGAATATGATCACAGAGCCGTACAAGTTAACTCGTCAATGCGAAGTTACTGCCATTCT CATCTTATTTGGGCTCCCTAGGCTACTGACTGGTTCGATTCTAGCTCACGAGATGATGCACGCGTG GATGCGGCTGAAAGGATTCCGGACACTGAGCCAAGACATTGAAGAAGGTATATGTCAAGTGATGGC TCATAAGTGGATAGAAGCCGAGTTAGCTGCTGGTTCAAGAAACAGCAGTGCTGCATCATCATCATC ATCGTCATCATCTTCTTCTTCTACTAGAGGACTGAAGAAGGGACCAAAATCGCAGTACGAGAGGAA GCTTGGCGAGTTTTTCAAGCACCAGATCGAGTCTGATGCGTCTCCGGTTTATGGAGACGGGTTCAG GGCAGGGCGGCTAGCGGTTAACAAGTATGGTTTGCGAAAAACGCTTGAGCATATACAGATGACCGG TAGATTCCCGGTTTAA

[0597] ( daltt8f ael ) -1 triple mutant protein sequence

[0598] DAI ( SEQ ID NO : 82 ) :

[0599] MGWFNKIFKGSNQRLRVGNEHNHNGYYENYPNAHASHDEPSADTDADRDEPQTQESSTSEDTSNDQ

[0600] ENEDIDRAIALSLLEENQGQKNTGGKYVMVDEDEQLARAIQESMIVGNSPRHKSGSTYDHGNAYGT

[0601] ADLFGNGHMHGGGNVYANGDIYYPRPVAFSMDFRICAGCNMEIGHGRFLNCLNSLWHPECFRCYGC

[0602] SHPISEYEFSTSGNYPFHKACYRERYHPKCDVCNHFIPTNPAGLIEYRAHPFWIQKYCPSHEHDST

[0603] PRCCSCERMEPRNTGYIELNDGRKLCLECLDSAVMDTSQCQPLYLQIQDFYEGLNNEGRAGSSTAL G

[0604] ( dal tt8fael ) -2 triple mutant nucleic acid sequence :

[0605] DAI ( -A in PS3 in DAI ; stacked in tt8fael ) ( SEQ ID NO : 83 ) :

[0606] ATGGGTTGGTTCAACAAGATCTTCAAAGGCTCTAACCAAAGGCTCCGGGTGGGGAATGAGCACAAT

[0607] CACAATGGCTATTACGAGAACTATCCAAATGCGCATGCTTCACACGACGAGCCTAGTGCGGATACA

[0608] GATGCTGATCGTGATGAACCTCAGACGCAGGAATCATCTACATCTGAGGATACGTCGAATGACCAG

[0609] GAAAATGAAGACATAGACCGTGCAATCGCGTTGTCTCTTTTAGAAGAGAATCAAGGACAGAAAAAT

[0610] ACAGGCGGGAAATACGTAATGGTGGATGAAGATGAGCAGCTTGCTAGAGCCATACAAGAAAGTATG

[0611] ATAGTTGGGAATTCACCGCGTCACAAAAGTGGAAGTACGTATGATCATGGGAATGCATATGGGACT

[0612] GCAGATTTATTTGGGAATGGTCACATGCATGGAGGAGGAAATGTATATGCAAATGGAGATATTTAT

[0613] TACCCAAGACCCGTTGCTTTTTCAATGGATTTCAGGATTTGTGCTGGCTGCAATATGGAGATTGGC

[0614] CATGGAAGATTTCTGAATTGCCTTAACTCACTATGGCATCCAGAATGTTTTCGATGTTATGGCTGT

[0615] AGTCACCCGATTTCTGAGTACGAGTTTTCAACATCTGGGAACTACCCTTTTCACAAGGCTTGTTAC

[0616] AGGGAGAGATATCATCCAAAATGTGATGTCTGCAACCACTTTATTCCAACAAATCCTGCTGGTCTT

[0617] ATTGAATATAGGGCACACCCATTTTGGATCCAGAAGTATTGCCCTTCTCACGAACACGATTCCACC

[0618] CCAAGATGTTGCAGTTGTGAAAGAATGGAGCCACGGAATACGGGATATATTGAGCTTAATGATGGA CGTAAACTTTGCCTCGAGTGTCTGGACTCAGCGGTCATGGACACAAGTCAATGTCAACCTTTGTAC TTGCAGATACAAGATTTCTATGAAGGGCTTAACATGAAGGTAGAGCAGGAAGTTCCACTGCTCTTG

[0619] GTTGAGAGGCAAGCACTTAACGAAGCCAGAGAAGGTGAAAAGAATGGTCACTATCACATGCCAGAG

[0620] ACAAGAGGACTGTGTCTTTCAGAAGAACAAACTGTTAGTACTATAAGAAAGCGATCAAAGGGAAAT

[0621] TGGGGTGGGAATATGATCACAGAGCCGTACAAGTTAACTCGTCAATGCGAAGTTACTGCCATTCTC

[0622] ATCTTATTTGGGCTCCCTAGGCTACTGACTGGTTCGATTCTAGCTCACGAGATGATGCACGCGTGG

[0623] ATGCGGCTGAAAGGATTCCGGACACTGAGCCAAGACATTGAAGAAGGTATATGTCAAGTGATGGCT CATAAGTGGATAGAAGCCGAGTTAGCTGCTGGTTCAAGAAACAGCAGTGCTGCATCATCATCATCA TCGTCATCATCTTCTTCTTCTACTAGAGGACTGAAGAAGGGACCAAAATCGCAGTACGAGAGGAAG

[0624] CTTGGCGAGTTTTTCAAGCACCAGATCGAGTCTGATGCGTCTCCGGTTTATGGAGACGGGTTCAGG

[0625] GCAGGGCGGCTAGCGGTTAACAAGTATGGTTTGCGAAAAACGCTTGAGCATATACAGATGACCGGT AGATTCCCGGTTTAA ( daltt8f ael ) -2 triple mutant protein sequence :

[0626] DAI ( SEQ ID NO : 84 ) :

[0627] MGWFNKIFKGSNQRLRVGNEHNHNGYYENYPNAHASHDEPSADTDADRDEPQTQESSTSEDTSNDQ

[0628] ENEDIDRAIALSLLEENQGQKNTGGKYVMVDEDEQLARAIQESMIVGNSPRHKSGSTYDHGNAYGT ADLFGNGHMHGGGNVYANGDIYYPRPVAFSMDFRICAGCIWRLAMEDF

[0629] ( darl tt8fael ) -1 triple mutant nucleic acid sequence : darl ( +T ( in PS5 ; stacked in ttSfael ) ( SEQ ID NO : 85 ) :

[0630] ATGGGGTGGCTTACTAAAATCCTTAAAGGCTCAAGCCATAAATTCTCGGATGGACAAGTTAACAGA

[0631] AGATATAGAGATGATAGGAACTTGGAAAGTTCTCGTCTTTCTGCGGATGGATCTGATTTTGACAAA

[0632] GAAGAGATTGAATGTGCTATTGCACTCTCTCTTTCAGAACAAGAACATGTAATTCCACAAGATGAC

[0633] AAAGGAAAACAAGTAATCGAATACAAATCTGAAACCGAAGAAGATGAGGATGAGGATGAGGACGAA GATGAAGAATACACGAGAGCTCAGCTAGAAGCAGCGGAAGAAGAGGAAAGACGGGCAGCTCAAGCT CAAATCGAGGAAGAAGAGAAACGGCGAGCTGAAGCTGAATTAGAAGAAATAGAGAAACAGCTTGCG

[0634] AAAGCTCGACTAGAAGAGGAAGAAGTTAGGCGTACCAAAGCGCAATTGGAGGAAGACGAGCAACTA

[0635] GCTAAGGCCATTCAAGAAAGTATGGTTGTGGGATCTCCTCCTCGGTATGATTCTGGAAGTATGTTT

[0636] CAACCATTCCCTTTCCTTAATCCATCTAGTCACAGGGGTTGCCTAGCTGAGATTGGACATGGAAGG

[0637] TTTCTGAGTTGCATGGGTGGCGTTTGGCATCCTGAATGTTTCTGCTGCCATGCTTGTGATAAGCCC

[0638] ATCATAGATTATGAGTTCTCAATGTCCGGGAATCGGCCTTATCACAAGCTATGTTACAAGGAGCAG CATCATCCTAAATGTGATGTTTGCCATAACTTTATTCCGACGAATTCAGCTGGTCTCATTGAGTAC AGGGCACATCCCTTTTGGATGCAAAAGTATTGTCCTTCACATGAGCGTGATGGAACTCCTCGTTGC TGTAGCTGTGAGCGAATGGAGCCAAAAGATACAAAATATCTCATTCTTGATGATGGTCGAAAACTG TGTCTTGAATGTCTCGACTCAGCCATTATGGACACTCACGAATGCCAACCATTGTACCTTGAGATA CGTGAGTTTTATGAAGGCTTACACATGAAAGTAGAACAGCAGATTCCTATGCTCTTGGTGGAGAGA TCAGCTTTAAACGAAGCTATGGAAGGAGAGAAACACGGACATCATCATTTACCTGAAACCAGAGGA CTCTGTTTGTCTGAGGAACAAACTGTCACAACGGTGTTAAGGAGACCAAGAATTGGGGCAGGCTAC AAATTGATAGACATGATCACAGAGCCTTGCAGGCTGATACGTCGTTGTGAAGTCACTGCAATTCTC ATTCTATATGGACTTCCTCGTTTGTTAACTGGATCAATCCTTGCTCATGAGATGATGCATGCCTGG CTTTCGACTAAATGGGTATCCGAATCTTAGGCCAGAAGTTGAAGAAGGAATATGTCAGGTTTTGGC TCACATGTGGTTGGAATCTGAGACTTATGCTGGCTCTACATTAGTAGATATCGCATCTTCTTCATC ATCATCATCATCGGCTGTGGTCACTGCATCGTCAAAGAAAGGTGAGAGGTCTGACTTTGAGAAGAA ACTTGGTGAGTTTTTCAAGCACCAAATAGAGTCGGATTCTTCGTCGGCATATGGGGATGGGTTCAG GCAAGGTAACCAAGCAGTTCTTACGCACGGTCTGAGGCGAACCCTCGACCATATTCGCTTGACCGG TACATTTCCTTAA

[0639] ( darl tt8fael ) -1 triple mutant protein sequence :

[0640] DARI ( SEQ ID NO : 86 ) :

[0641] MGWLTKILKGSSHKFSDGQVNRRYRDDRNLESSRLSADGSDFDKEEIECAIALSLSEQEHVIPQDD

[0642] KGKQVIEYKSETEEDEDEDEDEDEEYTRAQLEAAEEEERRAAQAQIEEEEKRRAEAELEEIEKQLA

[0643] KARLEEEEVRRTKAQLEEDEQLAKAIQESMVVGSPPRYDSGSMFQPFPFLNPSSHRGCLAEIGHGR

[0644] FLSCMGGVWHPECFCCHACDKPIIDYEFSMSGNRPYHKLCYKEQHHPKCDVCHNFIPTNSAGLIEY

[0645] RAHPFWMQKYCPSHERDGTPRCCSCERMEPKDTKYLILDDGRKLCLECLDSAIMDTHECQPLYLEI

[0646] REFYEGLHMKVEQQIPMLLVERSALNEAMEGEKHGHHHLPETRGLCLSEEQTVTTVLRRPRIGAGY KLIDMITEPCRLIRRCEVTAILILYGLPRLLTGSILAHEMMHAWLSTKWVSES

[0647] ( darl ttSfael } -2 triple mutant nucleic acid sequences : darl ( -4bp in PS3 ; stacked in tt8fael ) ( SEQ ID NO : 87 ) :

[0648] ATGGGGTGGCTTACTAAAATCCTTAAAGGCTCAAGCCATAAATTCTCGGATGGACAAGTTAACAGA

[0649] AGATATAGAGATGATAGGAACTTGGAAAGTTCTCGTCTTTCTGCGGATGGATCTGATTTTGACAAA GAAGAGATTGAATGTGCTATTGCACTCTCTCTTTCAGAACAAGAACATGTAATTCCACAAGATGAC AAAGGAAAACAAGTAATCGAATACAAATCTGAAACCGAAGAAGATGAGGATGAGGATGAGGACGAA GATGAAGAATACACGAGAGCTCAGCTAGAAGCAGCGGAAGAAGAGGAAAGACGGGCAGCTCAAGCT

[0650] CAAATCGAGGAAGAAGAGAAACGGCGAGCTGAAGCTGAATTAGAAGAAATAGAGAAACAGCTTGCG

[0651] AAAGCTCGACTAGAAGAGGAAGAAGTTAGGCGTACCAAAGCGCAATTGGAGGAAGACGAGCAACTA GCTAAGGCCATTCAAGAAAGTATGGTTGTGGGATCTCCTCCTCGGTATGATTCTGGAAGTATGTTT CAACCATTCCCTTTCCTTAATCCATCTAGTCACAGGGGTTGCCTAGCTGAGATTGGACATGGAAGG

[0652] TTTCTGAGTTGCATGGGTGGCGTTTGGCATCCTGAATGTTTCTGCTGCCATGCTTGTGATAAGCCC

[0653] ATCATAGATTATGAGTTCTCAATGTCCGGGAACCTTATCACAAGCTATGTTACAAGGAGCAGCATC

[0654] ATCCTAAATGTGATGTTTGCCATAACTTTATTCCGACGAATTCAGCTGGTCTCATTGAGTACAGGG

[0655] CACATCCCTTTTGGATGCAAAAGTATTGTCCTTCACATGAGCGTGATGGAACTCCTCGTTGCTGTA

[0656] GCTGTGAGCGAATGGAGCCAAAAGATACAAAATATCTCATTCTTGATGATGGTCGAAAACTGTGTC

[0657] TTGAATGTCTCGACTCAGCCATTATGGACACTCACGAATGCCAACCATTGTACCTTGAGATACGTG

[0658] AGTTTTATGAAGGCTTACACATGAAAGTAGAACAGCAGATTCCTATGCTCTTGGTGGAGAGATCAG

[0659] CTTTAAACGAAGCTATGGAAGGAGAGAAACACGGACATCATCATTTACCTGAAACCAGAGGACTCT

[0660] GTTTGTCTGAGGAACAAACTGTCACAACGGTGTTAAGGAGACCAAGAATTGGGGCAGGCTACAAAT

[0661] TGATAGACATGATCACAGAGCCTTGCAGGCTGATACGTCGTTGTGAAGTCACTGCAATTCTCATTC

[0662] TATATGGACTTCCTCGTTTGTTAACTGGATCAATCCTTGCTCATGAGATGATGCATGCCTGGCTTC

[0663] GACTAAATGGGTATCCGAATCTTAGGCCAGAAGTTGAAGAAGGAATATGTCAGGTTTTGGCTCACA

[0664] TGTGGTTGGAATCTGAGACTTATGCTGGCTCTACATTAGTAGATATCGCATCTTCTTCATCATCAT

[0665] CATCATCGGCTGTGGTCACTGCATCGTCAAAGAAAGGTGAGAGGTCTGACTTTGAGAAGAAACTTG

[0666] GTGAGTTTTTCAAGCACCAAATAGAGTCGGATTCTTCGTCGGCATATGGGGATGGGTTCAGGCAAG GTAACCAAGCAGTTCTTACGCACGGTCTGAGGCGAACCCTCGACCATATTCGCTTGACCGGTACAT TTCCTTAA

[0667] ( darl tt8fael ) -2 triple mutant protein sequence :

[0668] DARI ( SEQ ID NO : 88 ) :

[0669] MGWLTKILKGSSHKFSDGQVNRRYRDDRNLESSRLSADGSDFDKEEIECAIALSLSEQEHVIPQDD

[0670] KGKQVIEYKSETEEDEDEDEDEDEEYTRAQLEAAEEEERRAAQAQIEEEEKRRAEAELEEIEKQLA

[0671] KARLEEEEVRRTKAQLEEDEQLAKAIQESMVVGSPPRYDSGSMFQPFPFLNPSSHRGCLAEIGHGR

[0672] FLSCMGGVWHPECFCCHACDKPIIDYEFSMSGNLITSYVTRSSIILNVMFAITLFRRIQLVSLSTG

[0673] HIPFGCKSIVLHMSVMELLVAVAVSEWSQKIQNISFLMMVENCVLNVSTQPLWTLTNANHCTLRYV SFMKAYT

[0674] ( daldarl tt8fael ) -1 quadruple mutant nucleic acid sequences : da l ( inversion of 283bp between PSA and PSB stacked in tt8fael ) ( SEQ ID NO : 89 ) :

[0675] ATGGGTTGGTTCAACAAGATCTTCAAAGGCTCTAACCAAAGGCTCCGGGTGGGGAATGAGCACAAT CACAATGGCTATTACGAGAACTATCCAAATGCGCATGCTTCACACGACGAGCCTAGTGCGGATACA GATGCTGATCGTGATGAACCTCAGACGCAGGAATCATCTACATCTGAGGATACGTCGAATGACCAG GAAAATGAAGACATAGACCGTGCAATCGCGTTGTCTCTTTTAGAAGAGAATCAAGGACAGAAAAAT

[0676] ACAGGCGGGAAATACGTAATGGTGGATGAAGATGAGCAGCTTGCTAGAGCCATACAAGAAAGTATG

[0677] ATAGTTGGGAATTCACCGCGTCACAAAAGTGGAAGTACGTATGATCATGGGAATGCATATGGGACT

[0678] GCAGATTTATTTGGGAATGGTCACATGCATGGAGGAGGAAATGTATATGCAAATGGAGATATTTAT

[0679] TACCCAAGACCCGTTGCTTTTTCAATGGATTTCAGGATTTGTGCTGGCTGCAATATGGAGATTGGC

[0680] CATGGAAGATTTCTGAATTGCCTTAACTCACTATGGCATCCAGAATGTTTTCGATGTTATGGCTGT

[0681] AGTCACCCGATTTCTGAGTACGAGTTTTCAACATCTGGGAACTACCCTTTTCACAAGGCTTGTTAC

[0682] AGGGAGAGATATCATCCAAAATGTGATGTCTGCAACCACTTTATTCCAACAAATCCTGCTGGTCTT

[0683] ATTGAATATAGGGCACACCCATTTTGGATCCAGAAGTATTGCCCTTCTCACGAACACGATTCCACC

[0684] CCAAGATGTTGCAGTTGTGAAAGAATGGAGCCACGGAATACGGGATATATTGAGCTTAATGATGGA

[0685] CGTAAACTTTGCCTCGAGTGTCTGGACTCAGCGGTCATGGACACAAGTCAATGTCAACCTTTGTAC TTGCAGATACAAGATTTCTATGAAGGGCTTAACATGTACGGCTCTGTGATCATATTCCCACCCCAA TTTCCCTTTGATCGCTTTCTTATAGTACTAACAGTTTGTTCTTCTGAAAGACACAGTCCTCTTGTC TCTGGCATGTGATAGTGACCCTAAGAGGCACACAATGAAAAACTCAGGAGAAGCAAAATGTGATGT ATATTCAAGTAATTACTAAGTAATGATTGTATTTTTTTACTCACATTCTTTTCACCTTCTCTGGCT TCGTTAAGTGCTTGCCTCTCAACCAAGAGCAGTGGAACTTCCTGCTCTACCTTCAAAGTTAACTCG TCAATGCGAAGTTACTGCCATTCTCATCTTATTTGGGCTCCCTAGGCTACTGACTGGTTCGATTCT AGCTCACGAGATGATGCACGCGTGGATGCGGCTGAAAGGATTCCGGACACTGAGCCAAGACATTGA AGAAGGTATATGTCAAGTGATGGCTCATAAGTGGATAGAAGCCGAGTTAGCTGCTGGTTCAAGAAA CAGCAGTGCTGCATCATCATCATCATCGTCATCATCTTCTTCTTCTACTAGAGGACTGAAGAAGGG ACCAAAATCGCAGTACGAGAGGAAGCTTGGCGAGTTTTTCAAGCACCAGATCGAGTCTGATGCGTC TCCGGTTTATGGAGACGGGTTCAGGGCAGGGCGGCTAGCGGTTAACAAGTATGGTTTGCGAAAAAC GCTTGAGCATATACAGATGACCGGTAGATTCCCGGTTTAA darl ( -857 bp between PS3 and PS4 ) ( SEQ ID NO : 91 ) :

[0686] ATGGGGTGGCTTACTAAAATCCTTAAAGGCTCAAGCCATAAATTCTCGGATGGACAAGTTAACAGA AGATATAGAGATGATAGGAACTTGGAAAGTTCTCGTCTTTCTGCGGATGGATCTGATTTTGACAAA GAAGAGATTGAATGTGCTATTGCACTCTCTCTTTCAGAACAAGAACATGTAATTCCACAAGATGAC AAAGGAAAACAAGTAATCGAATACAAATCTGAAACCGAAGAAGATGAGGATGAGGATGAGGACGAA GATGAAGAATACACGAGAGCTCAGCTAGAAGCAGCGGAAGAAGAGGAAAGACGGGCAGCTCAAGCT CAAATCGAGGAAGAAGAGAAACGGCGAGCTGAAGCTGAATTAGAAGAAATAGAGAAACAGCTTGCG AAAGCTCGACTAGAAGAGGAAGAAGTTAGGCGTACCAAAGCGCAATTGGAGGAAGACGAGCAACTA GCTAAGGCCATTCAAGAAAGTATGGTTGTGGGATCTCCTCCTCGGTATGATTCTGGAAGTATGTTT CAACCATTCCCTTTCCTTAATCCATCTAGTCACAGGGGTTGCCTAGCTGAGATTGGACATGGAAGG TTTCTGAGTTGCATGGGTGGCGTTTGGCATCCTGAATGTTTCTGCTGCCATGCTTGTGATAAGCCC AT C AT AGAT T AT GAGT T C T C AAT GT C CGGG AAT TGGGGCAGGC T AC AAAT T GAT AGAC AT GAT C AC AGAGCCTTGCAGGCTGATACGTCGTTGTGAAGTCACTGCAATTCTCATTCTATATGGACTTCCTCG TTTGTTAACTGGATCAATCCTTGCTCATGAGATGATGCATGCCTGGCTTCGACTAAATGGtaaGTA TCCGAATCTTAGGCCAGAAGTTGAAGAAGGAATATGTCAGGTTTTGGCTCACATGTGGTTGGAATC TGAGACTTATGCTGGCTCTACATTAGTAGATATCGCATCTTCTTCATCATCATCATCATCGGCTGT GGTCACTGCATCGTCAAAGAAAGGTGAGAGGTCTGACTTTGAGAAGAAACTTGGTGAGTTTTTCAA GCACCAAATAGAGTCGGATTCTTCGTCGGCATATGGGGATGGGTTCAGGCAAGGTAACCAAGCAGT TCTTACGCACGGTCTGAGGCGAACCCTCGACCATATTCGCTTGACCGGTACATTTCCTTAA

[0687] ( daldarltt 8f ae l ) -1 protein sequences

[0688] DAI ( SEQ ID NO : 90 ) :

[0689] MGWFNKI FKGSNQRLRVGNEHNHNGYYENYPNAHASHDEPSADTDADRDEPQTQESSTSEDTSNDQ ENEDIDRAIALSLLEENQGQKNTGGKYVMVDEDEQLARAIQESMIVGNSPRHKSGSTYDHGNAYGT ADLFGNGHMHGGGNVYANGDIYYPRPVAFSMDFRICAGCNMEIGHGRFLNCLNSLWHPECFRCYGC SHPI SEYEFSTSGNYPFHKACYRERYHPKCDVCNHFIPTNPAGLIEYRAHPFWIQKYCPSHEHDST PRCCSCERMEPRNTGYIELNDGRKLCLECLDSAVMDTSQCQPLYLQIQDFYEGLNMYGSVI I FPPQ FPFDRFLIVLTVCSSERHSPLVSGM

[0690] DARI ( SEQ ID NO : 92 ) :

[0691] MGWLTKILKGSSHKFSDGQVNRRYRDDRNLESSRLSADGSDFDKEEIECAIALSLSEQEHVIPQDD KGKQVIEYKSETEEDEDEDEDEDEEYTRAQLEAAEEEERRAAQAQIEEEEKRRAEAELEEIEKQLA KARLEEEEVRRTKAQLEEDEQLAKAIQESMVVGSPPRYDSGSMFQPFPFLNPSSHRGCLAEIGHGR FLSCMGGVWHPECFCCHACDKPI IDYEFSMSGN

[0692] ( da ldarl tt8fael ) -2 quadruple mutant nucleic acid sequences da l ( -285bp between PSA and PSB stacked in ttSfael ) ( SEQ ID

[0693] NO : 93 ) :

[0694] ATGGGTTGGTTCAACAAGATCTTCAAAGGCTCTAACCAAAGGCTCCGGGTGGGGAATGAGCACAAT CACAATGGCTATTACGAGAACTATCCAAATGCGCATGCTTCACACGACGAGCCTAGTGCGGATACA GATGCTGATCGTGATGAACCTCAGACGCAGGAATCATCTACATCTGAGGATACGTCGAATGACCAG GAAAATGAAGACATAGACCGTGCAATCGCGTTGTCTCTTTTAGAAGAGAATCAAGGACAGAAAAAT ACAGGCGGGAAATACGTAATGGTGGATGAAGATGAGCAGCTTGCTAGAGCCATACAAGAAAGTATG ATAGTTGGGAATTCACCGCGTCACAAAAGTGGAAGTACGTATGATCATGGGAATGCATATGGGACT GCAGATTTATTTGGGAATGGTCACATGCATGGAGGAGGAAATGTATATGCAAATGGAGATATTTAT TACCCAAGACCCGTTGCTTTTTCAATGGATTTCAGGATTTGTGCTGGCTGCAATATGGAGATTGGC CATGGAAGATTTCTGAATTGCCTTAACTCACTATGGCATCCAGAATGTTTTCGATGTTATGGCTGT AGTCACCCGATTTCTGAGTACGAGTTTTCAACATCTGGGAACTACCCTTTTCACAAGGCTTGTTAC AGGGAGAGATATCATCCAAAATGTGATGTCTGCAACCACTTTATTCCAACAAATCCTGCTGGTCTT ATTGAATATAGGGCACACCCATTTTGGATCCAGAAGTATTGCCCTTCTCACGAACACGATTCCACC CCAAGATGTTGCAGTTGTGAAAGAATGGAGCCACGGAATACGGGATATATTGAGCTTAATGATGGA CGTAAACTTTGCCTCGAGTGTCTGGACTCAGCGGTCATGGACACAAGTCAATGTCAACCTTTGTAC TTGCAGATACAAGATTTCTATGAAGGGCTTAACAAGTTAACTCGTCAATGCGAAGTTACTGCCATT CTCATCTTATTTGGGCTCCCTAGGCTACTGACTGGTTCGATTCTAGCTCACGAGATGATGCACGCG TGGATGCGGCTGAAAGGATTCCGGACACTGAGCCAAGACATTGAAGAAGGTATATGTCAAGTGATG GCTCATAAGTGGATAGAAGCCGAGTTAGCTGCTGGTTCAAGAAACAGCAGTGCTGCATCATCATCA TCATCGTCATCATCTTCTTCTTCTACTAGAGGACTGAAGAAGGGACCAAAATCGCAGTACGAGAGG AAGCTTGGCGAGTTTTTCAAGCACCAGATCGAGTCTGATGCGTCTCCGGTTTATGGAGACGGGTTC AGGGCAGGGCGGCTAGCGGTTAACAAGTATGGTTTGCGAAAAACGCTTGAGCATATACAGATGACC GGTAGATTCCCGGTTTAA darl ( 857bp between PS3 and PS 4 stacked in tt8fael ) ( SEQ ID

[0695] NO : 95 ) :

[0696] ATGGGGTGGCTTACTAAAATCCTTAAAGGCTCAAGCCATAAATTCTCGGATGGACAAGTTAACAGA AGATATAGAGATGATAGGAACTTGGAAAGTTCTCGTCTTTCTGCGGATGGATCTGATTTTGACAAA GAAGAGATTGAATGTGCTATTGCACTCTCTCTTTCAGAACAAGAACATGTAATTCCACAAGATGAC AAAGGAAAACAAGTAATCGAATACAAATCTGAAACCGAAGAAGATGAGGATGAGGATGAGGACGAA GATGAAGAATACACGAGAGCTCAGCTAGAAGCAGCGGAAGAAGAGGAAAGACGGGCAGCTCAAGCT CAAATCGAGGAAGAAGAGAAACGGCGAGCTGAAGCTGAATTAGAAGAAATAGAGAAACAGCTTGCG AAAGCTCGACTAGAAGAGGAAGAAGTTAGGCGTACCAAAGCGCAATTGGAGGAAGACGAGCAACTA GCTAAGGCCATTCAAGAAAGTATGGTTGTGGGATCTCCTCCTCGGTATGATTCTGGAAGTATGTTT CAACCATTCCCTTTCCTTAATCCATCTAGTCACAGGGGTTGCCTAGCTGAGATTGGACATGGAAGG TTTCTGAGTTGCATGGGTGGCGTTTGGCATCCTGAATGTTTCTGCTGCCATGCTTGTGATAAGCCC AT C AT AGAT T AT GAGT T C T C AAT GT C CGGG AAT TGGGGCAGGC T AC AAAT T GAT AGAC AT GAT C AC AGAGCCTTGCAGGCTGATACGTCGTTGTGAAGTCACTGCAATTCTCATTCTATATGGACTTCCTCG TTTGTTAACTGGATCAATCCTTGCTCATGAGATGATGCATGCCTGGCTTCGACTAAATGGtaaGTA TCCGAATCTTAGGCCAGAAGTTGAAGAAGGAATATGTCAGGTTTTGGCTCACATGTGGTTGGAATC TGAGACTTATGCTGGCTCTACATTAGTAGATATCGCATCTTCTTCATCATCATCATCATCGGCTGT GGTCACTGCATCGTCAAAGAAAGGTGAGAGGTCTGACTTTGAGAAGAAACTTGGTGAGTTTTTCAA GCACCAAATAGAGTCGGATTCTTCGTCGGCATATGGGGATGGGTTCAGGCAAGGTAACCAAGCAGT TCTTACGCACGGTCTGAGGCGAACCCTCGACCATATTCGCTTGACCGGTACATTTCCTTAA

[0697] ( da ldarl tt8fael ) -2 quadruple mutant protein sequences :

[0698] DAI ( SEQ ID NO : 94 ) :

[0699] MGWFNKI FKGSNQRLRVGNEHNHNGYYENYPNAHASHDEPSADTDADRDEPQTQESSTSEDTSNDQ ENEDIDRAIALSLLEENQGQKNTGGKYVMVDEDEQLARAIQESMIVGNSPRHKSGSTYDHGNAYGT ADLFGNGHMHGGGNVYANGDIYYPRPVAFSMDFRICAGCNMEIGHGRFLNCLNSLWHPECFRCYGC SHPI SEYEFSTSGNYPFHKACYRERYHPKCDVCNHFIPTNPAGLIEYRAHPFWIQKYCPSHEHDST PRCCSCERMEPRNTGYIELNDGRKLCLECLDSAVMDTSQCQPLYLQIQDFYEGLNKLTRQCEVTAI LILFGLPRLLTGSILAHEMMHAWMRLKGFRTLSQDIEEGICQVMAHKWIEAELAAGSRNSSAASSS SSSSSSSSTRGLKKGPKSQYERKLGEFFKHQIESDASPVYGDGFRAGRLAVNKYGLRKTLEHIQMT

[0700] GRFPV

[0701] DARI ( SEQ ID NO : 96 ) :

[0702] MGWLTKILKGSSHKFSDGQVNRRYRDDRNLESSRLSADGSDFDKEEIECAIALSLSEQEHVIPQDD

[0703] KGKQVIEYKSETEEDEDEDEDEDEEYTRAQLEAAEEEERRAAQAQIEEEEKRRAEAELEEIEKQLA

[0704] KARLEEEEVRRTKAQLEEDEQLAKAIQESMVVGSPPRYDSGSMFQPFPFLNPSSHRGCLAEIGHGR FLSCMGGVWHPECFCCHACDKPIIDYEFSMSGN

[0705] ( daldarl tt8fael ) -3 quadruple mutant nucleic acid sequences : da l ( inversion of 283bp between PSA and PSB , +A in PSB in DAI stacked in tt8fael ) ( SEQ ID NO : 97 ) :

[0706] ATGGGTTGGTTCAACAAGATCTTCAAAGGCTCTAACCAAAGGCTCCGGGTGGGGAATGAGCACAAT

[0707] CACAATGGCTATTACGAGAACTATCCAAATGCGCATGCTTCACACGACGAGCCTAGTGCGGATACA

[0708] GATGCTGATCGTGATGAACCTCAGACGCAGGAATCATCTACATCTGAGGATACGTCGAATGACCAG

[0709] GAAAATGAAGACATAGACCGTGCAATCGCGTTGTCTCTTTTAGAAGAGAATCAAGGACAGAAAAAT

[0710] ACAGGCGGGAAATACGTAATGGTGGATGAAGATGAGCAGCTTGCTAGAGCCATACAAGAAAGTATG

[0711] ATAGTTGGGAATTCACCGCGTCACAAAAGTGGAAGTACGTATGATCATGGGAATGCATATGGGACT

[0712] GCAGATTTATTTGGGAATGGTCACATGCATGGAGGAGGAAATGTATATGCAAATGGAGATATTTAT

[0713] TACCCAAGACCCGTTGCTTTTTCAATGGATTTCAGGATTTGTGCTGGCTGCAATATGGAGATTGGC

[0714] CATGGAAGATTTCTGAATTGCCTTAACTCACTATGGCATCCAGAATGTTTTCGATGTTATGGCTGT

[0715] AGTCACCCGATTTCTGAGTACGAGTTTTCAACATCTGGGAACTACCCTTTTCACAAGGCTTGTTAC

[0716] AGGGAGAGATATCATCCAAAATGTGATGTCTGCAACCACTTTATTCCAACAAATCCTGCTGGTCTT

[0717] ATTGAATATAGGGCACACCCATTTTGGATCCAGAAGTATTGCCCTTCTCACGAACACGATTCCACC

[0718] CCAAGATGTTGCAGTTGTGAAAGAATGGAGCCACGGAATACGGGATATATTGAGCTTAATGATGGA

[0719] CGTAAACTTTGCCTCGAGTGTCTGGACTCAGCGGTCATGGACACAAGTCAATGTCAACCTTTGTAC

[0720] TTGCAGATACAAGATTTCTATGAAGGGCTTAACATGTACGGCTCTGTGATCATATTCCCACCCCAA

[0721] TTTCCCTTTGATCGCTTTCTTATAGTACTAACAGTTTGTTCTTCTGAAAGACACAGTCCTCTTGTC

[0722] TCTGGCATGTGATAGTGACCCTAAGAGGCACACAATGAAAAACTCAGGAGAAGCAAAATGTGATGT

[0723] ATATTCAAGTAATTACTAAGTAATGATTGTATTTTTTTACTCACATTCTTTTCACCTTCTCTGGCT

[0724] TCGTTAAGTGCTTGCCTCTCAACCAAGAGCAGTGGAACTTCCTGCTCTACCTTCAAAGTTAACTCG

[0725] TCAATGCGAAGTTACTGCCATTCTCATCTTATTTGGGCTCCCTAGGCTACTGACTGGTTCGATTCT

[0726] AGCTCACGAGATGATGCACGCGTGGATGCGGCTGAAAGGATTCCGGACACTGAGCCAAGACATTGA

[0727] AGAAGGTATATGTCAAGTGATGGCTCATAAGTGGATAGAAGCCGAGTTAGCTGCTGGTTCAAGAAA

[0728] CAGCAGTGCTGCATCATCATCATCATCGTCATCATCTTCTTCTTCTACTAGAGGACTGAAGAAGGG

[0729] ACCAAAATCGCAGTACGAGAGGAAGCTTGGCGAGTTTTTCAAGCACCAGATCGAGTCTGATGCGTC

[0730] TCCGGTTTATGGAGACGGGTTCAGGGCAGGGCGGCTAGCGGTTAACAAGTATGGTTTGCGAAAAAC

[0731] GCTTGAGCATATACAGATGACCGGTAGATTCCCGGTTTAA darl ( inversion of 857 bp between PS3 and PS4 stacked in tt8fael)

[0732] ( SEQ ID NO : 99 ) :

[0733] ATGGGGTGGCTTACTAAAATCCTTAAAGGCTCAAGCCATAAATTCTCGGATGGACAAGTTAACAGA

[0734] AGATATAGAGATGATAGGAACTTGGAAAGTTCTCGTCTTTCTGCGGATGGATCTGATTTTGACAAA

[0735] GAAGAGATTGAATGTGCTATTGCACTCTCTCTTTCAGAACAAGAACATGTAATTCCACAAGATGAC

[0736] AAAGGAAAACAAGTAATCGAATACAAATCTGAAACCGAAGAAGATGAGGATGAGGATGAGGACGAA

[0737] GATGAAGAATACACGAGAGCTCAGCTAGAAGCAGCGGAAGAAGAGGAAAGACGGGCAGCTCAAGCT

[0738] CAAATCGAGGAAGAAGAGAAACGGCGAGCTGAAGCTGAATTAGAAGAAATAGAGAAACAGCTTGCG

[0739] AAAGCTCGACTAGAAGAGGAAGAAGTTAGGCGTACCAAAGCGCAATTGGAGGAAGACGAGCAACTA

[0740] GCTAAGGCCATTCAAGAAAGTATGGTTGTGGGATCTCCTCCTCGGTATGATTCTGGAAGTATGTTT

[0741] CAACCATTCCCTTTCCTTAATCCATCTAGTCACAGGGGTTGCCTAGCTGAGATTGGACATGGAAGG

[0742] TTTCTGAGTTGCATGGGTGGCGTTTGGCATCCTGAATGTTTCTGCTGCCATGCTTGTGATAAGCCC ATCATAGATTATGAGTTCTCAATGTCCGGGATCTTGGTCTCCTTAACACCTACAAGAAACTCTTTC

[0743] TGATTCAAACACAATTTGTGGCGGATAGAAATAGCTGCTTACGAAAGTGAGAGCAATTTACCGTTG

[0744] TGACAGTTTGTTCCTCAGACAAACAGAGTCCTCTGGTTTCAGGTAAATGATGATGTCCCTGCAACA

[0745] AAAATAAATAGAAAGTAAGTTAAAAATAAATGAGCAAGAGCAAAAAGAGTGTTTATGTAAGCAACA

[0746] TAGGATAAATAAAGAGCGGTTTTTACGTGTTTCTCTCCTTCCATAGCTTCGTTTAAAGCTGATCTC

[0747] TCCACCAAGAGCATAGGAATCTGCTGTTCTACTTTCATGTGTAAGCCTTCATAAAACTCACGTATC

[0748] TCAAGGTACAATGGTTGGCATTCGTGAGTGTCCATAATGGCTGAGTCGAGACATTCAAGACACAGT

[0749] TTTCGACCATCATCAAGAATGAGATATTTTGTATCTTTTGGCTGTAACCGGTGAGCAAACACACAG

[0750] AACAACATCAAATCTCAAAAACGTCTCTTAGAATTGTTTCCCTATTAGTAGATATATCATATCGAA

[0751] ACTGACCTCCATTCGCTCACAGCTACAGCAACGAGGAGTTCCATCACGCTCATGTGAAGGACAATA

[0752] CTTTTGCATCCAAAAGGGATGTGCCCTGTACTCAATGAGACCAGCTGAATTCGTCGGAATCTATAG

[0753] GAGTTAATTTCACGCATACACATTTAAGCTTTGGTACCACTTGATAACATCGTTTGCAGAAGATGA

[0754] TATCAATAAAAAGGTCGGAATCACTCACAAAGTTATGGCAAACATCACATTTAGGATGATGCTGCT

[0755] CCTTGTAACATAGCTTGTGATAAGGCCGATATTGGGGCAGGCTACAAATTGATAGACATGATCACA

[0756] GAGCCTTGCAGGCTGATACGTCGTTGTGAAGTCACTGCAATTCTCATTCTATATGGACTTCCTCGT

[0757] TTGTTAACTGGATCAATCCTTGCTCATGAGATGATGCATGCCTGGCTTCGACTAAATGGGTATCCG

[0758] AATCTTAGGCCAGAAGTTGAAGAAGGAATATGTCAGGTTTTGGCTCACATGTGGTTGGAATCTGAG

[0759] ACTTATGCTGGCTCTACATTAGTAGATATCGCATCTTCTTCATCATCATCATCATCGGCTGTGGTC

[0760] ACTGCATCGTCAAAGAAAGGTGAGAGGTCTGACTTTGAGAAGAAACTTGGTGAGTTTTTCAAGCAC C AAAT AGAGT CGGATT CT TCGT CGGC AT AT GGGGATGGGTT CAGGCAAGGT AACC AAGC AGTT CT T ACGCACGGTCTGAGGCGAACCCTCGACCATATTCGCTTGACCGGTACATTTCCTTAA

[0761] ( daldarltt 8f ae l ) -3 quadruple mutant protein sequences :

[0762] DAI ( SEQ ID NO : 98 ) :

[0763] MGWFNKI FKGSNQRLRVGNEHNHNGYYENYPNAHASHDEPSADTDADRDEPQTQESSTSEDTSNDQ

[0764] ENEDIDRAIALSLLEENQGQKNTGGKYVMVDEDEQLARAIQESMIVGNSPRHKSGSTYDHGNAYGT

[0765] ADLFGNGHMHGGGNVYANGDIYYPRPVAFSMDFRICAGCNMEIGHGRFLNCLNSLWHPECFRCYGC

[0766] SHPI SEYEFSTSGNYPFHKACYRERYHPKCDVCNHFIPTNPAGLIEYRAHPFWIQKYCPSHEHDST

[0767] PRCCSCERMEPRNTGYIELNDGRKLCLECLDSAVMDTSQCQPLYLQIQDFYEGLNMYGSVI I FPPQ FPFDRFLIVLTVCSSERHSPLVSGM

[0768] DARI ( SEQ ID NO : 100 ) :

[0769] MGWLTKILKGSSHKFSDGQVNRRYRDDRNLESSRLSADGSDFDKEEIECAIALSLSEQEHVIPQDD

[0770] KGKQVIEYKSETEEDEDEDEDEDEEYTRAQLEAAEEEERRAAQAQIEEEEKRRAEAELEEIEKQLA

[0771] KARLEEEEVRRTKAQLEEDEQLAKAIQESMVVGSPPRYDSGSMFQPFPFLNPSSHRGCLAEIGHGR FLSCMGGVWHPECFCCHACDKPI IDYEFSMSGILVSLTPTRNS F

[0772] ( up! 3t t8fael -1 ) -1 triple mutant nucleic acid sequences : up!3 ( -326bp between PSI and PS2 , -4bp in PS4 ; stacked in t t8fael ) ( SEQ ID NO : 101 ) :

[0773] ATGGAAACTCGGAGCCGCAAGCGGGCGGAGGCGACCTCAACTGCCCCATCTTCTTCTTCTTCTTCA

[0774] CCTCCTCCTCCCTCAGGCCCCACCACCCGCAGCAAACGCGCTCGTCTTTCCTCTTCCTCTTCCTCC

[0775] TCTGTTGCCGCTACCACCACTGCACCTTCCTCTTCCACCCGCTCTCGCTCTTCTCGCTCCGCCGCC

[0776] GCCGCTGCTACTCCCATGGACACCTCCACCGAGTCTTCTGGATTTCGCCGAGGCGGACGAGGTAAC

[0777] AGGGGTAACGACAACAGTAATTCTGACAAGGGCAAGGAGAAGGAGCACGACGTTAGGATTAGGGAT

[0778] AGAGAAAGAGAAAGAGACCGAGCCAGAGAACAACTCAACATGGATGCTGCCGCTGCTGCTGCCGCC

[0779] GCTGACGACGACGACGACAATGACAGCGAGGATGGCAACGGGGGTTTCATGCATCCCAACATGAGC

[0780] TCCGCCAGCAGTGCTTTCTTGTGCCGCCGTTGTACATTACGGCGCTGTTTCATGCTTTGTCGCCAG

[0781] ATTGCTAACCATCGAGTACATGGACTTGGCCGAGCAGTCTCTGCAAGCTCTCAAAAAGATATCTCA

[0782] GGAGCACCCAACTGCCTGTTTGCGAGCTGGCGCTCTGATGGCAGTGCTCTCATATCTTGATTTCTT CTCCACTGGAGTCCAGCGTGTAGCACTATCTACTGCTGCAAATATGTGCAAGAAATTACCTTCTGA

[0783] TGCATCTGATTATGTTATGGAAGCTGTACCATTACTGACAAACCTACTTCAGTATCATGATTCGAA

[0784] GGTTTTGGAATATGCTTCTATATGTCTGACTCGGATTGCTGAAGCATTTGCTTCGCACCCTGAGAA

[0785] ATTGGATGAATTATGCAACCATGGCCTTGTGACTCAAGCTGCGTCTCTTATTTCCACTACCAACTC

[0786] AGGAGGTGGGCAAGCATCTCTGGGCGTTTCAACACGGGGTTAATCCGATTACTTTCCACCTGTGCG

[0787] AGCGGCTCACCTCTTGGATTCAGGACATTGCTTCTTCTCGGCATTAGTAGCATTCTTAAGGATATT

[0788] CTGTCGGGTCCTGGTGTCTCTGCTAATGCATCTGTATCTCCAGCACTGAGCAGGCCCGCAGATCAG

[0789] ATCTTTGAGATAGTCAACCTAGCGAATGAGCTCCTCCCTCCATTGCCAGAAGGAGTTATCTCCCTT

[0790] CCTACTAGCACAAACACTTTGGTGAAAGGTTCAGGCCAAAAGAAATCTTCTCCAAGTACTTCAGGA

[0791] AAACAAGACGATTCACCAAAAGTTTCACCTAGAGAAAAATTACTTGCTGATCAACCTGAACTTCTG

[0792] CAGAAATTTGGACTGGATCTTCTTCCAGTTTTAGTGCAGATTTATGGTTCTAGTGTCAATGGTACT

[0793] ATTCGCCATAAATGTCTGTCAGTTATCGGAAAGTTGATGTATTTCAGCACTTCAGAAATGATTCAA

[0794] TCTCTAATTGGTGACACAAATATATCGAGCTTCTTGGCTGGTGTATTGGCATGGAAAGATCCACAA

[0795] GTCTTGGTTCCTGCTCTACAAGTTGCAGAAATTCTGATGGAAAAGCTTCCTGAAACATTCTCGAAA

[0796] GTGTTTGTGAGGGAAGGGGTGGTTCATGCTGTAGATCAACTTGTCTTGGTTGGTAAACCTATTTCT

[0797] CATGCTTCACCTACTGATAAGGAAAATGACTGTGTGCCAGGATCTGCACGATCTAGGCGATATAGA

[0798] CGGCGAAGTAGTAATGCCAGTTCTGATGGAAATCAGTCAGAAGAGCTTAAGAATTCTGCGTCGGTT

[0799] AACGTAGGTGCAAACCATAATTCCCTAGATTCTCCTACCTCTAGCTTCTTGCTAAAGGAAACAGTT

[0800] AGCTCCTGTGCCAAAGCATTCAAAGACAAGTACTTCCCGTCTGATGGCGGGGATCTCGATGTTGGA

[0801] GTTACAGATGATCTCTTACATCTAAAGAATCTTTGCACGAAGCTAACTGCTAGTATAGATGAGCAT

[0802] AAAGTGAAAGGAAAGGGAAAATCCAAAGCCTCTGGGCCATGCCTTGGCGATTTTTCTGCTAGCAAG

[0803] GAAGAATACTTGATTGGTGTCATTTCTGAGATACTTGGCGAGCTTAGCAAAGGGGATGGAGTCTCA

[0804] ACTTTTGAGTTTATTGGCAGTGGTGTGGTTGCAGCTTTGCTTGACTATTTCTCTTGTGGGTACTTT

[0805] TCCAAAGAGAAGATCTCCGAGGTTAATTTGCCCAAACTTCGCCAAGATGGGCTCAGAAGATTCAAA

[0806] GCTTTTCTAGAAGTCGCACTTCCGTCTGATGGTAATGAGGGAAAGATCCCTCCTATGACAGTTTTG

[0807] ATTCAGAAACTTCAAGATGCTTTATCTTCACTGGAACGCTTTCCCGTCGTCCTTAGTCATCCCTCA

[0808] AGGTCATTAGGTGGAAGTGCTCGTCTCTCATCTGGATTGAGTGCTTTGGCACATCCTTTAAAGTTG

[0809] CGATTATGCCGTGCACCTGGAGAGAAGACACTACGTGATTACTCCTCCAATATTGTTCTTATAGAT

[0810] CCACTGGCAAGCATTGCAGCGGTAGAAGAATTTCTTTGGCCCCGAGTTCAACGGAGTGAATCTGGT

[0811] ATGAAGCATGCAGCGCCTGCTGGAAATACAGAGCCAGGCACGTTACCTAGCGGTGCTGGTGTTTCA

[0812] TCACCATCCTCGTCAACTCCGGCTTCCACCACACGTCGTCATTCTTCTAGATCTAGATCAGCAATT

[0813] AACATAGGTGATACCTCAAAGAAAGAAGCTGTGCATGAGAAAGGTACCAGCTCATCTAAAGGAAAA

[0814] GGTAAAGGCGTTATGAAGCCCGTTCCAGCTGATAAGGGGCCTCAAACAAGGAGCAGTGCTCAAAGA

[0815] AGAGCTGTCCTTGACAAAGATACACATATGAAACCAGCTAGCGGAGACTCCAGCTCCGAGGATGAG

[0816] GAATTGGAAATAGCCCCTGTCGACATTGATGATGCCTTGGTGATTGAAGAGGATGACATGTCTGAT

[0817] GATGAAGATGATGATAATGACGATGTGTTGGATGACAGTCTTCCCATGTGCACGCCTGATAAAGTG

[0818] CATGATGTAAAATTGGGGGACGCAGTGGATGATGACGGTGGTGCTGGTCTGGCCTCTAGCGGGCGA

[0819] CAGATGAATCCAGCTTCAGGTGGCACTAGTGGAGCAGCAGCAGCGAGGGGATCTGATTCTATTGAT

[0820] GCTGGCATTGGGAATTCTTACGGTTCTAGGGGTGCACTCTCCTTTGCTGCTGCGGCAATGGCTGGG

[0821] CTTGGAGCTGCCAGTGGTAGAGGTATCAGGGGAAGTAGAGACCTGCATGGGCGTACCCTATATCGA

[0822] AGTTCCGACGAGCCCTCTAAGTTATTATTTACTGCAGGAGGAAAGCAACTTAGTAGGCATTTGACG

[0823] ATATATCAGGCTGTGCAGCGACAACTTATGCTAGATGAAGACGATGATGAAAGGTTCGGTGGCAGC

[0824] GATTTCATGTCAAGTGATGGAAGCAGATTCAATGATATTTACACCATCATGTACCAGAGGCCGGAG

[0825] AGTCAAGCGAATAGGTTGTCTGTTGGTGCAGCAAGTTCTACCACACTATCAAAATCCACTAAATCT

[0826] GCTACTGCCAATTCTAGCGTAGAATCTCAGTCATATAAGGCATCTCTTTTGGATAGTATCTTACAA

[0827] GGGGAGCTTCCGTGCGATCTTGAGAAGTCAAATTCTACTTATAATGTTCTGGCGCTGTTGCGTGTA

[0828] TTAGAGGGGTTAAATCAGCTTGGCCCTCGGTTGAGAGCCCAAACTGTTTCTGATCGTTTCGCAGAG

[0829] GGTAAAATTACAAGTCTAGATGATCTGAAGACAACTGCTGCTGCTAAGATTTCTCATGAAGAATTC

[0830] ATCAATAGCAAACTTACACCCAAATTAGCTCGACAGATCCAGGATGCGCTTGCTTTGTGCAGTGGT

[0831] AGTCTTCCCTCTTGGTGCTACCAGTTGACTAGAGCATGCCCGTTTTTGTTTCCGTTTCAGACCCGG

[0832] AGACAGTATTTCTATTCAACTGCTTTTGGGTTGTCCCGCGCATTGAACCGCTTGCAGCAGCAGCAA GGTGCTGACGGCAGTGGGTCTACAAATGAACGAGAGATGAGAATAGGGAGGTTGCAGCGCCAGAAA

[0833] GTCCGTGTATCCCGAAATAGAATATTAGATTCTGCTGCGAAAGTTATGGAGATGTATTCTAGCCAA

[0834] AAAGCTGTGCTTGAAGTAGAATATTTTGGTGAAGTTGGTACTGGTCTAGGCCCTACACTTGAGTTT

[0835] TACACACTCCTAAGCCATGATCTACAAAAGGTTGCGCTTGGGATGTGGAGATCAAATTCTGGTGAC

[0836] ATGGTATCTATGCAAATTGATAGAGGTGAGATTGAAGACGGGAAATCATCCGCAGCTAGGGACAGA

[0837] GATATAGTTCAGGCACCGCTTGGGTTGTTTCCTCGACCTTGGCCCTCTACAGCTGACGTATCTGAA

[0838] GGTAGTCGGTTTCATAAAGTCATTGAATATTTCCGGCTTTTAGGGCGTGTGATGGCAAAAGCACTT

[0839] CAAGATGGACGGCTACTGGACGTCCCATTGAGTACAGCGTTTTATAAGCTTATTCTTGGTCAAGAG

[0840] CTTGATTTGCATGATATCATATTATTTGATACCGAGCTTGGCAAGACTTTGCAAGAGTTTCGTGTT

[0841] CTTGTTGGCCGCAAGCACTATCTGGAGGCAGTAGGCGGTGACAATTGCAGCGCTGGTTCTGATTTA

[0842] TGTTTACGTGGATCCCGTATAGAAGATCTCTGCTTGGACTTCACGCTTCCTGGCTATCCTGAATAC

[0843] ATTCTGAGACCAGGAGATGAAATTGTTGATATGAATAGTCTTGAAGAGTATATATCCCTTGTCGTT

[0844] GATGCTACTGTCAAGAGAGGAGTTACCCGGCAGATTGAAGCCTTTAGATCTGGATTCAATCAGGTG

[0845] TTTGACGTAACATCTCTACAAATATTCACCCCTTCCGAGCTGGACTATTTGCTGTGTGGTCGTAGA

[0846] GAGTTGTGGGAGGCGGAGACTCTTGCTGAACATATCAAGTTTGATCACGGTTATACTGCTAAAAGT

[0847] CCGGCAATCATTAATTTACTGGAGATCATGGGAGAACTTACAGCGGATCAGCAGAGGGCTTTCTGC

[0848] CAATTTGTAACTGGAGCTCCTAGGCTTCCTCCTGGTGGGCTAGCTGTTTTGAATCCAAAGCTGACG

[0849] ATTGTGAGAAAGCATTCATCAACCTCAAGTGCGGCAGCGAACGGAACAGGGGCTTCGGAGACGGCA

[0850] GACGATGATCTTCCCAGCGTAATGACTTGCGCCAACTATCTTAAGCTCCCTCCTTATTCGACAAAG

[0851] GAAATCATGTACAAGAAACTGCTCTACGCCATCAACGAAGGGCAAGGATCGTTCGACCTGTCATAA

[0852] ( upl3tt8 fael ) -1 triple mutant protein sequence :

[0853] UPL3 ( SEQ ID NO : 102 ) :

[0854] METRSRKRAEATSTAPSSSSSSPPPPSGPTTRSKRARLSSSSSSSVAATTTAPSSSTRSRSSRSAA

[0855] AAATPMDTSTESSGFRRGGRGNRGNDNSNSDKGKEKEHDVRIRDRERERDRAREQLNMDAAAAAAA

[0856] ADDDDDNDSEDGNGGFMHPNMSSASSAFLCRRCTLRRCFMLCRQIANHRVHGLGRAVSASSQKDIS GAPNCL FAS WRS DG SAL I S

[0857] ( upl3tt8fael ) -2 triple mutant nucleic acid sequence : up!3 ( -4bp in PS4 in UPL3; stacked in tt8fael ) ( SEQ ID NO : 103 ) :

[0858] ATGGAAACTCGGAGCCGCAAGCGGGCGGAGGCGACCTCAACTGCCCCATCTTCTTCTTCTTCTTCA

[0859] CCTCCTCCTCCCTCAGGCCCCACCACCCGCAGCAAACGCGCTCGTCTTTCCTCTTCCTCTTCCTCC

[0860] TCTGTTGCCGCTACCACCACTGCACCTTCCTCTTCCACCCGCTCTCGCTCTTCTCGCTCCGCCGCC

[0861] GCCGCTGCTACTCCCATGGACACCTCCACCGAGTCTTCTGGATTTCGCCGAGGCGGACGAGGTAAC

[0862] AGGGGTAACGACAACAGTAATTCTGACAAGGGCAAGGAGAAGGAGCACGACGTTAGGATTAGGGAT

[0863] AGAGAAAGAGAAAGAGACCGAGCCAGAGAACAACTCAACATGGATGCTGCCGCTGCTGCTGCCGCC

[0864] GCTGACGACGACGACGACAATGACAGCGAGGATGGCAACGGGGGTTTCATGCATCCCAACATGAGC

[0865] TCCGCCAGCAGTGCTTTACAAGGTTTGCTGAGGAAGCTTGGAGCTGGCCTCGATGACTTGCTTCCT

[0866] TCCTCTGGTATCGGCTCTGCTTCTTCTTCCCACTTAAATGGGAGGATGAAGAAGATACTCTCTGGC

[0867] TTGCGCTCCGAAGGAGAAGAGGGAAAGCAGGTCGAGGCTTTAACCCAGCTATGCGAGATGCTATCC

[0868] ATTGGGACTGAAGACTCATTAAGCACCTTCTCTGTTGATTCCTTCGTCCCTGTTCTCGTTGGTCTA

[0869] CTAAACCATGAAAGCAATCCCGACATAATGCTTCTTGCGGCCAGGGCTCTTACCCATCTATGTGAT

[0870] GTTTTGCCGTCTTCTTGTGCCGCCGTTGTACATTACGGCGCTGTTTCATGCTTTGTCGCCAGATTG

[0871] CTAACCATCGAGTACATGGACTTGGCCGAGCAGTCTCTGCAAGCTCTCAAAAAGATATCTCAGGAG

[0872] CACCCAACTGCCTGTTTGCGAGCTGGCGCTCTGATGGCAGTGCTCTCATATCTTGATTTCTTCTCC

[0873] ACTGGAGTCCAGCGTGTAGCACTATCTACTGCTGCAAATATGTGCAAGAAATTACCTTCTGATGCA

[0874] TCTGATTATGTTATGGAAGCTGTACCATTACTGACAAACCTACTTCAGTATCATGATTCGAAGGTT

[0875] TTGGAATATGCTTCTATATGTCTGACTCGGATTGCTGAAGCATTTGCTTCGCACCCTGAGAAATTG

[0876] GATGAATTATGCAACCATGGCCTTGTGACTCAAGCTGCGTCTCTTATTTCCACTACCAACTCAGGA

[0877] GGTGGGCAAGCATCTCTGGGCGTTTCAACACGGGGTTAATCCGATTACTTTCCACCTGTGCGAGCG

[0878] GCTCACCTCTTGGATTCAGGACATTGCTTCTTCTCGGCATTAGTAGCATTCTTAAGGATATTCTGT CGGGTCCTGGTGTCTCTGCTAATGCATCTGTATCTCCAGCACTGAGCAGGCCCGCAGATCAGATCT

[0879] TTGAGATAGTCAACCTAGCGAATGAGCTCCTCCCTCCATTGCCAGAAGGAGTTATCTCCCTTCCTA

[0880] CTAGCACAAACACTTTGGTGAAAGGTTCAGGCCAAAAGAAATCTTCTCCAAGTACTTCAGGAAAAC

[0881] AAGACGATTCACCAAAAGTTTCACCTAGAGAAAAATTACTTGCTGATCAACCTGAACTTCTGCAGA

[0882] AATTTGGACTGGATCTTCTTCCAGTTTTAGTGCAGATTTATGGTTCTAGTGTCAATGGTACTATTC

[0883] GCCATAAATGTCTGTCAGTTATCGGAAAGTTGATGTATTTCAGCACTTCAGAAATGATTCAATCTC

[0884] TAATTGGTGACACAAATATATCGAGCTTCTTGGCTGGTGTATTGGCATGGAAAGATCCACAAGTCT

[0885] TGGTTCCTGCTCTACAAGTTGCAGAAATTCTGATGGAAAAGCTTCCTGAAACATTCTCGAAAGTGT

[0886] TTGTGAGGGAAGGGGTGGTTCATGCTGTAGATCAACTTGTCTTGGTTGGTAAACCTATTTCTCATG

[0887] CTTCACCTACTGATAAGGAAAATGACTGTGTGCCAGGATCTGCACGATCTAGGCGATATAGACGGC

[0888] GAAGTAGTAATGCCAGTTCTGATGGAAATCAGTCAGAAGAGCTTAAGAATTCTGCGTCGGTTAACG

[0889] TAGGTGCAAACCATAATTCCCTAGATTCTCCTACCTCTAGCTTCTTGCTAAAGGAAACAGTTAGCT

[0890] CCTGTGCCAAAGCATTCAAAGACAAGTACTTCCCGTCTGATGGCGGGGATCTCGATGTTGGAGTTA

[0891] CAGATGATCTCTTACATCTAAAGAATCTTTGCACGAAGCTAACTGCTAGTATAGATGAGCATAAAG

[0892] TGAAAGGAAAGGGAAAATCCAAAGCCTCTGGGCCATGCCTTGGCGATTTTTCTGCTAGCAAGGAAG

[0893] AATACTTGATTGGTGTCATTTCTGAGATACTTGGCGAGCTTAGCAAAGGGGATGGAGTCTCAACTT

[0894] TTGAGTTTATTGGCAGTGGTGTGGTTGCAGCTTTGCTTGACTATTTCTCTTGTGGGTACTTTTCCA

[0895] AAGAGAAGATCTCCGAGGTTAATTTGCCCAAACTTCGCCAAGATGGGCTCAGAAGATTCAAAGCTT

[0896] TTCTAGAAGTCGCACTTCCGTCTGATGGTAATGAGGGAAAGATCCCTCCTATGACAGTTTTGATTC

[0897] AGAAACTTCAAGATGCTTTATCTTCACTGGAACGCTTTCCCGTCGTCCTTAGTCATCCCTCAAGGT

[0898] CATTAGGTGGAAGTGCTCGTCTCTCATCTGGATTGAGTGCTTTGGCACATCCTTTAAAGTTGCGAT

[0899] TATGCCGTGCACCTGGAGAGAAGACACTACGTGATTACTCCTCCAATATTGTTCTTATAGATCCAC

[0900] TGGCAAGCATTGCAGCGGTAGAAGAATTTCTTTGGCCCCGAGTTCAACGGAGTGAATCTGGTATGA

[0901] AGCATGCAGCGCCTGCTGGAAATACAGAGCCAGGCACGTTACCTAGCGGTGCTGGTGTTTCATCAC

[0902] CATCCTCGTCAACTCCGGCTTCCACCACACGTCGTCATTCTTCTAGATCTAGATCAGCAATTAACA

[0903] TAGGTGATACCTCAAAGAAAGAAGCTGTGCATGAGAAAGGTACCAGCTCATCTAAAGGAAAAGGTA

[0904] AAGGCGTTATGAAGCCCGTTCCAGCTGATAAGGGGCCTCAAACAAGGAGCAGTGCTCAAAGAAGAG

[0905] CTGTCCTTGACAAAGATACACATATGAAACCAGCTAGCGGAGACTCCAGCTCCGAGGATGAGGAAT

[0906] TGGAAATAGCCCCTGTCGACATTGATGATGCCTTGGTGATTGAAGAGGATGACATGTCTGATGATG

[0907] AAGATGATGATAATGACGATGTGTTGGATGACAGTCTTCCCATGTGCACGCCTGATAAAGTGCATG

[0908] ATGTAAAATTGGGGGACGCAGTGGATGATGACGGTGGTGCTGGTCTGGCCTCTAGCGGGCGACAGA

[0909] TGAATCCAGCTTCAGGTGGCACTAGTGGAGCAGCAGCAGCGAGGGGATCTGATTCTATTGATGCTG

[0910] GCATTGGGAATTCTTACGGTTCTAGGGGTGCACTCTCCTTTGCTGCTGCGGCAATGGCTGGGCTTG

[0911] GAGCTGCCAGTGGTAGAGGTATCAGGGGAAGTAGAGACCTGCATGGGCGTACCCTATATCGAAGTT

[0912] CCGACGAGCCCTCTAAGTTATTATTTACTGCAGGAGGAAAGCAACTTAGTAGGCATTTGACGATAT

[0913] ATCAGGCTGTGCAGCGACAACTTATGCTAGATGAAGACGATGATGAAAGGTTCGGTGGCAGCGATT

[0914] TCATGTCAAGTGATGGAAGCAGATTCAATGATATTTACACCATCATGTACCAGAGGCCGGAGAGTC

[0915] AAGCGAATAGGTTGTCTGTTGGTGCAGCAAGTTCTACCACACTATCAAAATCCACTAAATCTGCTA

[0916] CTGCCAATTCTAGCGTAGAATCTCAGTCATATAAGGCATCTCTTTTGGATAGTATCTTACAAGGGG

[0917] AGCTTCCGTGCGATCTTGAGAAGTCAAATTCTACTTATAATGTTCTGGCGCTGTTGCGTGTATTAG

[0918] AGGGGTTAAATCAGCTTGGCCCTCGGTTGAGAGCCCAAACTGTTTCTGATCGTTTCGCAGAGGGTA

[0919] AAATTACAAGTCTAGATGATCTGAAGACAACTGCTGCTGCTAAGATTTCTCATGAAGAATTCATCA

[0920] ATAGCAAACTTACACCCAAATTAGCTCGACAGATCCAGGATGCGCTTGCTTTGTGCAGTGGTAGTC

[0921] TTCCCTCTTGGTGCTACCAGTTGACTAGAGCATGCCCGTTTTTGTTTCCGTTTCAGACCCGGAGAC

[0922] AGTATTTCTATTCAACTGCTTTTGGGTTGTCCCGCGCATTGAACCGCTTGCAGCAGCAGCAAGGTG

[0923] CTGACGGCAGTGGGTCTACAAATGAACGAGAGATGAGAATAGGGAGGTTGCAGCGCCAGAAAGTCC

[0924] GTGTATCCCGAAATAGAATATTAGATTCTGCTGCGAAAGTTATGGAGATGTATTCTAGCCAAAAAG

[0925] CTGTGCTTGAAGTAGAATATTTTGGTGAAGTTGGTACTGGTCTAGGCCCTACACTTGAGTTTTACA

[0926] CACTCCTAAGCCATGATCTACAAAAGGTTGCGCTTGGGATGTGGAGATCAAATTCTGGTGACATGG

[0927] TATCTATGCAAATTGATAGAGGTGAGATTGAAGACGGGAAATCATCCGCAGCTAGGGACAGAGATA

[0928] TAGTTCAGGCACCGCTTGGGTTGTTTCCTCGACCTTGGCCCTCTACAGCTGACGTATCTGAAGGTA GTCGGTTTCATAAAGTCATTGAATATTTCCGGCTTTTAGGGCGTGTGATGGCAAAAGCACTTCAAG ATGGACGGCTACTGGACGTCCCATTGAGTACAGCGTTTTATAAGCTTATTCTTGGTCAAGAGCTTG ATTTGCATGATATCATATTATTTGATACCGAGCTTGGCAAGACTTTGCAAGAGTTTCGTGTTCTTG TTGGCCGCAAGCACTATCTGGAGGCAGTAGGCGGTGACAATTGCAGCGCTGGTTCTGATTTATGTT TACGTGGATCCCGTATAGAAGATCTCTGCTTGGACTTCACGCTTCCTGGCTATCCTGAATACATTC TGAGACCAGGAGATGAAATTGTTGATATGAATAGTCTTGAAGAGTATATATCCCTTGTCGTTGATG CTACTGTCAAGAGAGGAGTTACCCGGCAGATTGAAGCCTTTAGATCTGGATTCAATCAGGTGTTTG ACGTAACATCTCTACAAATATTCACCCCTTCCGAGCTGGACTATTTGCTGTGTGGTCGTAGAGAGT TGTGGGAGGCGGAGACTCTTGCTGAACATATCAAGTTTGATCACGGTTATACTGCTAAAAGTCCGG CAATCATTAATTTACTGGAGATCATGGGAGAACTTACAGCGGATCAGCAGAGGGCTTTCTGCCAAT TTGTAACTGGAGCTCCTAGGCTTCCTCCTGGTGGGCTAGCTGTTTTGAATCCAAAGCTGACGATTG TGAGAAAGCATTCATCAACCTCAAGTGCGGCAGCGAACGGAACAGGGGCTTCGGAGACGGCAGACG ATGATCTTCCCAGCGTAATGACTTGCGCCAACTATCTTAAGCTCCCTCCTTATTCGACAAAGGAAA TCATGTACAAGAAACTGCTCTACGCCATCAACGAAGGGCAAGGATCGTTCGACCTGTCATAA

[0929] ( upl3tt8 f ael ) -2 triple mutant protein sequence :

[0930] UPL3 ( SEQ ID NO : 104 ) :

[0931] METRSRKRAEATSTAPSSSSSSPPPPSGPTTRSKRARLSSSSSSSVAATTTAPSSSTRSRSSRSAA

[0932] AAATPMDTSTESSGFRRGGRGNRGNDNSNSDKGKEKEHDVRIRDRERERDRAREQLNMDAAAAAAA ADDDDDNDSEDGNGGFMHPNMSSASSALQGLLRKLGAGLDDLLPSSGIGSASSSHLNGRMKKILSG LRSEGEEGKQVEALTQLCEMLSIGTEDSLSTFSVDSFVPVLVGLLNHESNPDIMLLAARALTHLCD VLPSSCAAVVHYGAVSCFVARLLTIEYMDLAEQSLQALKKISQEHPTACLRAGALMAVLSYLDFFS TGVQRVALSTAANMCKKLPSDASDYVMEAVPLLTNLLQYHDSKVLEYASICLTRIAEAFASHPEKL DELCNHGLVTQAASLISTTNSGGGQASLGVSTRG

[0933] ( upl3tt8fael ) -3 triple mutant nucleic acid sequences : up!3 ( -22bp in PS2 ; stacked in ttBfael ) ( SEQ ID NO : 105 ) : ATGGAAACTCGGAGCCGCAAGCGGGCGGAGGCGACCTCAACTGCCCCATCTTCTTCTTCTTCTTCA

[0934] CCTCCTCCTCCCTCAGGCCCCACCACCCGCAGCAAACGCGCTCGTCTTTCCTCTTCCTCTTCCTCC

[0935] TCTGTTGCCGCTACCACCACTGCACCTTCCTCTTCCACCCGCTCTCGCTCTTCTCGCTCCGCCGCC GCCGCTGCTACTCCCATGGACACCTCCACCGAGTCTTCTGGATTTCGCCGAGGCGGACGAGGTAAC AGGGGTAACGACAACAGTAATTCTGACAAGGGCAAGGAGAAGGAGCACGACGTTAGGATTAGGGAT AGAGAAAGAGAAAGAGACCGAGCCAGAGAACAACTCAACATGGATGCTGCCGCTGCTGCTGCCGCC GCTGACGACGACGACGACAATGACAGCGAGGATGGCAACGGGGGTTTCATGCATCCCAACATGAGC TCCGCCAGCAGTGCTTTACAAGGTTTGCTGAGGAAGCTTGGAGCTGGCCTCGATGACTTGCTTCCT TCCTCTGGTATCGGCTCTGCTTCTTCTTCCCACTTAAATGGGAGGATGAAGAAGATACTCTCTGGC TTGCGCTCCGAAGGAGAAGAGGGAAAGCAGGTCGAGGCTTTAACCCAGCTATGCGAGATGCTATCC ATTGGGACTGAAGACTCATTAAGCACCTTCTCTGTTGATTCCTTCGTCCCTGTTCTCGTTGGTCTA CTAAACCATGAAAGCAATCCCGACATAATGCTTCTTGCGGCCAGGGCTCTTACCCATCTTGTGCCG CCGTTGTACATTACGGCGCTGTTTCATGCTTTGTCGCCAGATTGCTAACCATCGAGTACATGGACT TGGCCGAGCAGTCTCTGCAAGCTCTCAAAAAGATATCTCAGGAGCACCCAACTGCCTGTTTGCGAG CTGGCGCTCTGATGGCAGTGCTCTCATATCTTGATTTCTTCTCCACTGGAGTCCAGCGTGTAGCAC TATCTACTGCTGCAAATATGTGCAAGAAATTACCTTCTGATGCATCTGATTATGTTATGGAAGCTG TACCATTACTGACAAACCTACTTCAGTATCATGATTCGAAGGTTTTGGAATATGCTTCTATATGTC TGACTCGGATTGCTGAAGCATTTGCTTCGCACCCTGAGAAATTGGATGAATTATGCAACCATGGCC TTGTGACTCAAGCTGCGTCTCTTATTTCCACTACCAACTCAGGAGGTGGGCAAGCATCTCTGGGCG TTTCAACATACACGGGGTTAATCCGATTACTTTCCACCTGTGCGAGCGGCTCACCTCTTGGATTCA GGACATTGCTTCTTCTCGGCATTAGTAGCATTCTTAAGGATATTCTGTCGGGTCCTGGTGTCTCTG CTAATGCATCTGTATCTCCAGCACTGAGCAGGCCCGCAGATCAGATCTTTGAGATAGTCAACCTAG CGAATGAGCTCCTCCCTCCATTGCCAGAAGGAGTTATCTCCCTTCCTACTAGCACAAACACTTTGG TGAAAGGTTCAGGCCAAAAGAAATCTTCTCCAAGTACTTCAGGAAAACAAGACGATTCACCAAAAG TTTCACCTAGAGAAAAATTACTTGCTGATCAACCTGAACTTCTGCAGAAATTTGGACTGGATCTTC TTCCAGTTTTAGTGCAGATTTATGGTTCTAGTGTCAATGGTACTATTCGCCATAAATGTCTGTCAG TTATCGGAAAGTTGATGTATTTCAGCACTTCAGAAATGATTCAATCTCTAATTGGTGACACAAATA TATCGAGCTTCTTGGCTGGTGTATTGGCATGGAAAGATCCACAAGTCTTGGTTCCTGCTCTACAAG TTGCAGAAATTCTGATGGAAAAGCTTCCTGAAACATTCTCGAAAGTGTTTGTGAGGGAAGGGGTGG TTCATGCTGTAGATCAACTTGTCTTGGTTGGTAAACCTATTTCTCATGCTTCACCTACTGATAAGG AAAATGACTGTGTGCCAGGATCTGCACGATCTAGGCGATATAGACGGCGAAGTAGTAATGCCAGTT CTGATGGAAATCAGTCAGAAGAGCTTAAGAATTCTGCGTCGGTTAACGTAGGTGCAAACCATAATT CCCTAGATTCTCCTACCTCTAGCTTCTTGCTAAAGGAAACAGTTAGCTCCTGTGCCAAAGCATTCA AAGACAAGTACTTCCCGTCTGATGGCGGGGATCTCGATGTTGGAGTTACAGATGATCTCTTACATC TAAAGAAT CT TT GCACGAAGCTAACT GOT AGT AT AGAT GAGCAT AAAGT GAAAGGAAAGGGAAAAT CCAAAGCCTCTGGGCCATGCCTTGGCGATTTTTCTGCTAGCAAGGAAGAATACTTGATTGGTGTCA TTTCTGAGATACTTGGCGAGCTTAGCAAAGGGGATGGAGTCTCAACTTTTGAGTTTATTGGCAGTG GTGTGGTTGCAGCTTTGCTTGACTATTTCTCTTGTGGGTACTTTTCCAAAGAGAAGATCTCCGAGG TTAATTTGCCCAAACTTCGCCAAGATGGGCTCAGAAGATTCAAAGCTTTTCTAGAAGTCGCACTTC CGTCTGATGGTAATGAGGGAAAGATCCCTCCTATGACAGTTTTGATTCAGAAACTTCAAGATGCTT TATCTTCACTGGAACGCTTTCCCGTCGTCCTTAGTCATCCCTCAAGGTCATTAGGTGGAAGTGCTC GTCTCTCATCTGGATTGAGTGCTTTGGCACATCCTTTAAAGTTGCGATTATGCCGTGCACCTGGAG AGAAGACACTACGTGATTACTCCTCCAATATTGTTCTTATAGATCCACTGGCAAGCATTGCAGCGG TAGAAGAATTTCTTTGGCCCCGAGTTCAACGGAGTGAATCTGGTATGAAGCATGCAGCGCCTGCTG GAAATACAGAGCCAGGCACGTTACCTAGCGGTGCTGGTGTTTCATCACCATCCTCGTCAACTCCGG CTTCCACCACACGTCGTCATTCTTCTAGATCTAGATCAGCAATTAACATAGGTGATACCTCAAAGA AAGAAGCTGTGCATGAGAAAGGTACCAGCTCATCTAAAGGAAAAGGTAAAGGCGTTATGAAGCCCG TTCCAGCTGATAAGGGGCCTCAAACAAGGAGCAGTGCTCAAAGAAGAGCTGTCCTTGACAAAGATA CACATATGAAACCAGCTAGCGGAGACTCCAGCTCCGAGGATGAGGAATTGGAAATAGCCCCTGTCG ACATTGATGATGCCTTGGTGATTGAAGAGGATGACATGTCTGATGATGAAGATGATGATAATGACG ATGTGTTGGATGACAGTCTTCCCATGTGCACGCCTGATAAAGTGCATGATGTAAAATTGGGGGACG CAGTGGATGATGACGGTGGTGCTGGTCTGGCCTCTAGCGGGCGACAGATGAATCCAGCTTCAGGTG GCACTAGTGGAGCAGCAGCAGCGAGGGGATCTGATTCTATTGATGCTGGCATTGGGAATTCTTACG GTTCTAGGGGTGCACTCTCCTTTGCTGCTGCGGCAATGGCTGGGCTTGGAGCTGCCAGTGGTAGAG GTATCAGGGGAAGTAGAGACCTGCATGGGCGTACCCTATATCGAAGTTCCGACGAGCCCTCTAAGT TATTATTTACTGCAGGAGGAAAGCAACTTAGTAGGCATTTGACGATATATCAGGCTGTGCAGCGAC AACTTATGCTAGATGAAGACGATGATGAAAGGTTCGGTGGCAGCGATTTCATGTCAAGTGATGGAA GCAGATTCAATGATATTTACACCATCATGTACCAGAGGCCGGAGAGTCAAGCGAATAGGTTGTCTG TTGGTGCAGCAAGTTCTACCACACTATCAAAATCCACTAAATCTGCTACTGCCAATTCTAGCGTAG AATCTCAGTCATATAAGGCATCTCTTTTGGATAGTATCTTACAAGGGGAGCTTCCGTGCGATCTTG AGAAGTCAAATTCTACTTATAATGTTCTGGCGCTGTTGCGTGTATTAGAGGGGTTAAATCAGCTTG GCCCTCGGTTGAGAGCCCAAACTGTTTCTGATCGTTTCGCAGAGGGTAAAATTACAAGTCTAGATG ATCTGAAGACAACTGCTGCTGCTAAGATTTCTCATGAAGAATTCATCAATAGCAAACTTACACCCA AATTAGCTCGACAGATCCAGGATGCGCTTGCTTTGTGCAGTGGTAGTCTTCCCTCTTGGTGCTACC AGTTGACTAGAGCATGCCCGTTTTTGTTTCCGTTTCAGACCCGGAGACAGTATTTCTATTCAACTG CTTTTGGGTTGTCCCGCGCATTGAACCGCTTGCAGCAGCAGCAAGGTGCTGACGGCAGTGGGTCTA CAAATGAACGAGAGATGAGAATAGGGAGGTTGCAGCGCCAGAAAGTCCGTGTATCCCGAAATAGAA TATTAGATTCTGCTGCGAAAGTTATGGAGATGTATTCTAGCCAAAAAGCTGTGCTTGAAGTAGAAT ATTTTGGTGAAGTTGGTACTGGTCTAGGCCCTACACTTGAGTTTTACACACTCCTAAGCCATGATC TACAAAAGGTTGCGCTTGGGATGTGGAGATCAAATTCTGGTGACATGGTATCTATGCAAATTGATA GAGGTGAGATTGAAGACGGGAAATCATCCGCAGCTAGGGACAGAGATATAGTTCAGGCACCGCTTG GGTTGTTTCCTCGACCTTGGCCCTCTACAGCTGACGTATCTGAAGGTAGTCGGTTTCATAAAGTCA TTGAATATTTCCGGCTTTTAGGGCGTGTGATGGCAAAAGCACTTCAAGATGGACGGCTACTGGACG TCCCATTGAGTACAGCGTTTTATAAGCTTATTCTTGGTCAAGAGCTTGATTTGCATGATATCATAT TATTTGATACCGAGCTTGGCAAGACTTTGCAAGAGTTTCGTGTTCTTGTTGGCCGCAAGCACTATC TGGAGGCAGTAGGCGGTGACAATTGCAGCGCTGGTTCTGATTTATGTTTACGTGGATCCCGTATAG

[0936] AAGATCTCTGCTTGGACTTCACGCTTCCTGGCTATCCTGAATACATTCTGAGACCAGGAGATGAAA TTGTTGATATGAATAGTCTTGAAGAGTATATATCCCTTGTCGTTGATGCTACTGTCAAGAGAGGAG TTACCCGGCAGATTGAAGCCTTTAGATCTGGATTCAATCAGGTGTTTGACGTAACATCTCTACAAA TATTCACCCCTTCCGAGCTGGACTATTTGCTGTGTGGTCGTAGAGAGTTGTGGGAGGCGGAGACTC TTGCTGAACATATCAAGTTTGATCACGGTTATACTGCTAAAAGTCCGGCAATCATTAATTTACTGG AGATCATGGGAGAACTTACAGCGGATCAGCAGAGGGCTTTCTGCCAATTTGTAACTGGAGCTCCTA GGCTTCCTCCTGGTGGGCTAGCTGTTTTGAATCCAAAGCTGACGATTGTGAGAAAGCATTCATCAA CCTCAAGTGCGGCAGCGAACGGAACAGGGGCTTCGGAGACGGCAGACGATGATCTTCCCAGCGTAA TGACTTGCGCCAACTATCTTAAGCTCCCTCCTTATTCGACAAAGGAAATCATGTACAAGAAACTGC TCTACGCCATCAACGAAGGGCAAGGATCGTTCGACCTGTCATAA

[0937] ( upl3tt8 fael ) -3 triple mutant protein sequence :

[0938] UPL3 ( SEQ ID NO : 106 ) :

[0939] METRSRKRAEATSTAPSSSSSSPPPPSGPTTRSKRARLSSSSSSSVAATTTAPSSSTRSRSSRSAA

[0940] AAATPMDTSTESSGFRRGGRGNRGNDNSNSDKGKEKEHDVRIRDRERERDRAREQLNMDAAAAAAA

[0941] ADDDDDNDSEDGNGGFMHPNMSSASSALQGLLRKLGAGLDDLLPSSGIGSASSSHLNGRMKKILSG LRSEGEEGKQVEALTQLCEMLSIGTEDSLSTFSVDSFVPVLVGLLNHESNPDIMLLAARALTHLVP PLYITALFHALSPDC da lupl3 double mutant nucleic acid sequences :

[0942] DAI ( inversion of 284bp between PSA and PSB , +T in PSB ) ( SEQ ID NO : 107 ) :

[0943] ATGGGTTGGTTCAACAAGATCTTCAAAGGCTCTAACCAAAGGCTCCGGGTGGGGAATGAGCACAAT CACAATGGCTATTACGAGAACTATCCAAATGCGCATGCTTCACACGACGAGCCTAGTGCGGATACA GATGCTGATCGTGATGAACCTCAGACGCAGGAATCATCTACATCTGAGGATACGTCGAATGACCAG GAAAATGAAGACATAGACCGTGCAATCGCGTTGTCTCTTTTAGAAGAGAATCAAGGACAGAAAAAT ACAGGCGGGAAATACGTAATGGTGGATGAAGATGAGCAGCTTGCTAGAGCCATACAAGAAAGTATG ATAGTTGGGAATTCACCGCGTCACAAAAGTGGAAGTACGTATGATCATGGGAATGCATATGGGACT GCAGATTTATTTGGGAATGGTCACATGCATGGAGGAGGAAATGTATATGCAAATGGAGATATTTAT TACCCAAGACCCGTTGCTTTTTCAATGGATTTCAGGATTTGTGCTGGCTGCAATATGGAGATTGGC CATGGAAGATTTCTGAATTGCCTTAACTCACTATGGCATCCAGAATGTTTTCGATGTTATGGCTGT AGTCACCCGATTTCTGAGTACGAGTTTTCAACATCTGGGAACTACCCTTTTCACAAGGCTTGTTAC AGGGAGAGATATCATCCAAAATGTGATGTCTGCAACCACTTTATTCCAACAAATCCTGCTGGTCTT ATTGAATATAGGGCACACCCATTTTGGATCCAGAAGTATTGCCCTTCTCACGAACACGATTCCACC CCAAGATGTTGCAGTTGTGAAAGAATGGAGCCACGGAATACGGGATATATTGAGCTTAATGATGGA CGTAAACTTTGCCTCGAGTGTCTGGACTCAGCGGTCATGGACACAAGTCAATGTCAACCTTTGTAC TTGCAGATACAAGATTTCTATGAAGGGCTTAACAGTACGGCTCTGTGATCATATTCCCACCCCAAT TTCCCTTTGATCGCTTTCTTATAGTACTAACAGTTTGTTCTTCTGAAAGACACAGTCCTCTTGTCT CTGGCATGTGATAGTGACCCTAAGAGGCACACAATGAAAAACTCAGGAGAAGCAAAATGTGATGTA TATTCAAGTAATTACTAAGTAATGATTGTATTTTTTTACTCACATTCTTTTCACCTTCTCTGGCTT CGTTAAGTGCTTGCCTCTCAACCAAGAGCAGTGGAACTTCCTGCTCTACCTTCATAAGTTAACTCG TCAATGCGAAGTTACTGCCATTCTCATCTTATTTGGGCTCCCTAGGCTACTGACTGGTTCGATTCT AGCTCACGAGATGATGCACGCGTGGATGCGGCTGAAAGGATTCCGGACACTGAGCCAAGACATTGA AGAAGGTATATGTCAAGTGATGGCTCATAAGTGGATAGAAGCCGAGTTAGCTGCTGGTTCAAGAAA CAGCAGTGCTGCATCATCATCATCATCGTCATCATCTTCTTCTTCTACTAGAGGACTGAAGAAGGG ACCAAAATCGCAGTACGAGAGGAAGCTTGGCGAGTTTTTCAAGCACCAGATCGAGTCTGATGCGTC TCCGGTTTATGGAGACGGGTTCAGGGCAGGGCGGCTAGCGGTTAACAAGTATGGTTTGCGAAAAAC GCTTGAGCATATACAGATGACCGGTAGATTCCCGGTTTAA upl3 ( -782bp between PS2 and PS4 ) ( SEQ ID NO : 109 ) :

[0944] ATGGAAACTCGGAGCCGCAAGCGGGCGGAGGCGACCTCAACTGCCCCATCTTCTTCTTCTTCTTCA CCTCCTCCTCCCTCAGGCCCCACCACCCGCAGCAAACGCGCTCGTCTTTCCTCTTCCTCTTCCTCC TCTGTTGCCGCTACCACCACTGCACCTTCCTCTTCCACCCGCTCTCGCTCTTCTCGCTCCGCCGCC GCCGCTGCTACTCCCATGGACACCTCCACCGAGTCTTCTGGATTTCGCCGAGGCGGACGAGGTAAC AGGGGTAACGACAACAGTAATTCTGACAAGGGCAAGGAGAAGGAGCACGACGTTAGGATTAGGGAT AGAGAAAGAGAAAGAGACCGAGCCAGAGAACAACTCAACATGGATGCTGCCGCTGCTGCTGCCGCC GCTGACGACGACGACGACAATGACAGCGAGGATGGCAACGGGGGTTTCATGCATCCCAACATGAGC TCCGCCAGCAGTGCTTTACAAGGTTTGCTGAGGAAGCTTGGAGCTGGCCTCGATGACTTGCTTCCT TCCTCTGGTATCGGCTCTGCTTCTTCTTCCCACTTAAATGGGAGGATGAAGAAGATACTCTCTGGC TTGCGCTCCGAAGGAGAAGAGGGAAAGCAGGTCGAGGCTTTAACCCAGCTATGCGAGATGCTATCC ATTGGGACTGAAGACTCATTAAGCACCTTCTCTGTTGATTCCTTCGTCCCTGTTCTCGTTGGTCTA CTAAACCATGAAAGCAATCCCGACATAATGCTTCTTGCGGCCAGGGCTCTTACCCATCTATGTGAT GTTTTGCCGTCACACGGGGTTAATCCGATTACTTTCCACCTGTGCGAGCGGCTCACCTCTTGGATT CAGGACATTGCTTCTTCTCGGCATTAGTAGCATTCTTAAGGATATTCTGTCGGGTCCTGGTGTCTC TGCTAATGCATCTGTATCTCCAGCACTGAGCAGGCCCGCAGATCAGATCTTTGAGATAGTCAACCT AGCGAATGAGCTCCTCCCTCCATTGCCAGAAGGAGTTATCTCCCTTCCTACTAGCACAAACACTTT GGTGAAAGGTTCAGGCCAAAAGAAATCTTCTCCAAGTACTTCAGGAAAACAAGACGATTCACCAAA AGTTTCACCTAGAGAAAAATTACTTGCTGATCAACCTGAACTTCTGCAGAAATTTGGACTGGATCT TCTTCCAGTTTTAGTGCAGATTTATGGTTCTAGTGTCAATGGTACTATTCGCCATAAATGTCTGTC AGTTATCGGAAAGTTGATGTATTTCAGCACTTCAGAAATGATTCAATCTCTAATTGGTGACACAAA TATATCGAGCTTCTTGGCTGGTGTATTGGCATGGAAAGATCCACAAGTCTTGGTTCCTGCTCTACA AGTTGCAGAAATTCTGATGGAAAAGCTTCCTGAAACATTCTCGAAAGTGTTTGTGAGGGAAGGGGT GGTTCATGCTGTAGATCAACTTGTCTTGGTTGGTAAACCTATTTCTCATGCTTCACCTACTGATAA GGAAAATGACTGTGTGCCAGGATCTGCACGATCTAGGCGATATAGACGGCGAAGTAGTAATGCCAG TTCTGATGGAAATCAGTCAGAAGAGCTTAAGAATTCTGCGTCGGTTAACGTAGGTGCAAACCATAA TTCCCTAGATTCTCCTACCTCTAGCTTCTTGCTAAAGGAAACAGTTAGCTCCTGTGCCAAAGCATT CAAAGACAAGTACTTCCCGTCTGATGGCGGGGATCTCGATGTTGGAGTTACAGATGATCTCTTACA TCTAAAGAATCTTTGCACGAAGCTAACTGCTAGTATAGATGAGCATAAAGTGAAAGGAAAGGGAAA ATCCAAAGCCTCTGGGCCATGCCTTGGCGATTTTTCTGCTAGCAAGGAAGAATACTTGATTGGTGT CATTTCTGAGATACTTGGCGAGCTTAGCAAAGGGGATGGAGTCTCAACTTTTGAGTTTATTGGCAG TGGTGTGGTTGCAGCTTTGCTTGACTATTTCTCTTGTGGGTACTTTTCCAAAGAGAAGATCTCCGA GGTTAATTTGCCCAAACTTCGCCAAGATGGGCTCAGAAGATTCAAAGCTTTTCTAGAAGTCGCACT TCCGTCTGATGGTAATGAGGGAAAGATCCCTCCTATGACAGTTTTGATTCAGAAACTTCAAGATGC TTTATCTTCACTGGAACGCTTTCCCGTCGTCCTTAGTCATCCCTCAAGGTCATTAGGTGGAAGTGC TCGTCTCTCATCTGGATTGAGTGCTTTGGCACATCCTTTAAAGTTGCGATTATGCCGTGCACCTGG AGAGAAGACACTACGTGATTACTCCTCCAATATTGTTCTTATAGATCCACTGGCAAGCATTGCAGC GGTAGAAGAATTTCTTTGGCCCCGAGTTCAACGGAGTGAATCTGGTATGAAGCATGCAGCGCCTGC TGGAAATACAGAGCCAGGCACGTTACCTAGCGGTGCTGGTGTTTCATCACCATCCTCGTCAACTCC GGCTTCCACCACACGTCGTCATTCTTCTAGATCTAGATCAGCAATTAACATAGGTGATACCTCAAA GAAAGAAGCTGTGCATGAGAAAGGTACCAGCTCATCTAAAGGAAAAGGTAAAGGCGTTATGAAGCC CGTTCCAGCTGATAAGGGGCCTCAAACAAGGAGCAGTGCTCAAAGAAGAGCTGTCCTTGACAAAGA TACACATATGAAACCAGCTAGCGGAGACTCCAGCTCCGAGGATGAGGAATTGGAAATAGCCCCTGT C GAG AT T GAT GAT GCC T T GGT GAT T G AAGAGGAT GAG AT GT CT GAT GAT GAAGAT GAT G AT AAT GA CGATGTGTTGGATGACAGTCTTCCCATGTGCACGCCTGATAAAGTGCATGATGTAAAATTGGGGGA CGCAGTGGATGATGACGGTGGTGCTGGTCTGGCCTCTAGCGGGCGACAGATGAATCCAGCTTCAGG TGGCACTAGTGGAGCAGCAGCAGCGAGGGGATCTGATTCTATTGATGCTGGCATTGGGAATTCTTA CGGTTCTAGGGGTGCACTCTCCTTTGCTGCTGCGGCAATGGCTGGGCTTGGAGCTGCCAGTGGTAG AGGTATCAGGGGAAGTAGAGACCTGCATGGGCGTACCCTATATCGAAGTTCCGACGAGCCCTCTAA GTTATTATTTACTGCAGGAGGAAAGCAACTTAGTAGGCATTTGACGATATATCAGGCTGTGCAGCG ACAACTTATGCTAGATGAAGACGATGATGAAAGGTTCGGTGGCAGCGATTTCATGTCAAGTGATGG AAGCAGATTCAATGATATTTACACCATCATGTACCAGAGGCCGGAGAGTCAAGCGAATAGGTTGTC

[0945] TGTTGGTGCAGCAAGTTCTACCACACTATCAAAATCCACTAAATCTGCTACTGCCAATTCTAGCGT

[0946] AGAATCTCAGTCATATAAGGCATCTCTTTTGGATAGTATCTTACAAGGGGAGCTTCCGTGCGATCT

[0947] TGAGAAGTCAAATTCTACTTATAATGTTCTGGCGCTGTTGCGTGTATTAGAGGGGTTAAATCAGCT

[0948] TGGCCCTCGGTTGAGAGCCCAAACTGTTTCTGATCGTTTCGCAGAGGGTAAAATTACAAGTCTAGA

[0949] TGATCTGAAGACAACTGCTGCTGCTAAGATTTCTCATGAAGAATTCATCAATAGCAAACTTACACC

[0950] CAAATTAGCTCGACAGATCCAGGATGCGCTTGCTTTGTGCAGTGGTAGTCTTCCCTCTTGGTGCTA

[0951] CCAGTTGACTAGAGCATGCCCGTTTTTGTTTCCGTTTCAGACCCGGAGACAGTATTTCTATTCAAC

[0952] TGCTTTTGGGTTGTCCCGCGCATTGAACCGCTTGCAGCAGCAGCAAGGTGCTGACGGCAGTGGGTC

[0953] TACAAATGAACGAGAGATGAGAATAGGGAGGTTGCAGCGCCAGAAAGTCCGTGTATCCCGAAATAG

[0954] AATATTAGATTCTGCTGCGAAAGTTATGGAGATGTATTCTAGCCAAAAAGCTGTGCTTGAAGTAGA

[0955] ATATTTTGGTGAAGTTGGTACTGGTCTAGGCCCTACACTTGAGTTTTACACACTCCTAAGCCATGA

[0956] TCTACAAAAGGTTGCGCTTGGGATGTGGAGATCAAATTCTGGTGACATGGTATCTATGCAAATTGA

[0957] TAGAGGTGAGATTGAAGACGGGAAATCATCCGCAGCTAGGGACAGAGATATAGTTCAGGCACCGCT

[0958] TGGGTTGTTTCCTCGACCTTGGCCCTCTACAGCTGACGTATCTGAAGGTAGTCGGTTTCATAAAGT

[0959] CATTGAATATTTCCGGCTTTTAGGGCGTGTGATGGCAAAAGCACTTCAAGATGGACGGCTACTGGA

[0960] CGTCCCATTGAGTACAGCGTTTTATAAGCTTATTCTTGGTCAAGAGCTTGATTTGCATGATATCAT

[0961] ATTATTTGATACCGAGCTTGGCAAGACTTTGCAAGAGTTTCGTGTTCTTGTTGGCCGCAAGCACTA

[0962] TCTGGAGGCAGTAGGCGGTGACAATTGCAGCGCTGGTTCTGATTTATGTTTACGTGGATCCCGTAT

[0963] AGAAGATCTCTGCTTGGACTTCACGCTTCCTGGCTATCCTGAATACATTCTGAGACCAGGAGATGA

[0964] AATTGTTGATATGAATAGTCTTGAAGAGTATATATCCCTTGTCGTTGATGCTACTGTCAAGAGAGG

[0965] AGTTACCCGGCAGATTGAAGCCTTTAGATCTGGATTCAATCAGGTGTTTGACGTAACATCTCTACA

[0966] AATATTCACCCCTTCCGAGCTGGACTATTTGCTGTGTGGTCGTAGAGAGTTGTGGGAGGCGGAGAC

[0967] TCTTGCTGAACATATCAAGTTTGATCACGGTTATACTGCTAAAAGTCCGGCAATCATTAATTTACT

[0968] GGAGATCATGGGAGAACTTACAGCGGATCAGCAGAGGGCTTTCTGCCAATTTGTAACTGGAGCTCC

[0969] TAGGCTTCCTCCTGGTGGGCTAGCTGTTTTGAATCCAAAGCTGACGATTGTGAGAAAGCATTCATC

[0970] AACCTCAAGTGCGGCAGCGAACGGAACAGGGGCTTCGGAGACGGCAGACGATGATCTTCCCAGCGT

[0971] AATGACTTGCGCCAACTATCTTAAGCTCCCTCCTTATTCGACAAAGGAAATCATGTACAAGAAACT GCTCTACGCCATCAACGAAGGGCAAGGATCGTTCGACCTGTCATAA

[0972] DA1UPL3 double mutant protein sequences :

[0973] DAI ( SEQ ID NO : 108 ) :

[0974] MGWFNKIFKGSNQRLRVGNEHNHNGYYENYPNAHASHDEPSADTDADRDEPQTQESSTSEDTSNDQ

[0975] ENEDIDRAIALSLLEENQGQKNTGGKYVMVDEDEQLARAIQESMIVGNSPRHKSGSTYDHGNAYGT

[0976] ADLFGNGHMHGGGNVYANGDIYYPRPVAFSMDFRICAGCNMEIGHGRFLNCLNSLWHPECFRCYGC

[0977] SHPISEYEFSTSGNYPFHKACYRERYHPKCDVCNHFIPTNPAGLIEYRAHPFWIQKYCPSHEHDST PRCCSCERMEPRNTGYIELNDGRKLCLECLDSAVMDTSQCQPLYLQIQDFYEGLN

[0978] UPL3 ( SEQ ID NO : 110 ) :

[0979] METRSRKRAEATSTAPSSSSSSPPPPSGPTTRSKRARLSSSSSSSVAATTTAPSSSTRSRSSRSAA

[0980] AAATPMDTSTESSGFRRGGRGNRGNDNSNSDKGKEKEHDVRIRDRERERDRAREQLNMDAAAAAAA

[0981] ADDDDDNDSEDGNGGFMHPNMSSASSALQGLLRKLGAGLDDLLPSSGIGSASSSHLNGRMKKILSG

[0982] LRSEGEEGKQVEALTQLCEMLSIGTEDSLSTFSVDSFVPVLVGLLNHESNPDIMLLAARALTHLCD VLPS da ldarlupld triple mutant nucleic acid sequences : da l ( ~297bp between PSA and PSB ) ( SEQ ID NO : 111 ) :

[0983] ATGGGTTGGTTCAACAAGATCTTCAAAGGCTCTAACCAAAGGCTCCGGGTGGGGAATGAGCACAAT

[0984] CACAATGGCTATTACGAGAACTATCCAAATGCGCATGCTTCACACGACGAGCCTAGTGCGGATACA

[0985] GATGCTGATCGTGATGAACCTCAGACGCAGGAATCATCTACATCTGAGGATACGTCGAATGACCAG

[0986] GAAAATGAAGACATAGACCGTGCAATCGCGTTGTCTCTTTTAGAAGAGAATCAAGGACAGAAAAAT ACAGGCGGGAAATACGTAATGGTGGATGAAGATGAGCAGCTTGCTAGAGCCATACAAGAAAGTATG ATAGTTGGGAATTCACCGCGTCACAAAAGTGGAAGTACGTATGATCATGGGAATGCATATGGGACT GCAGATTTATTTGGGAATGGTCACATGCATGGAGGAGGAAATGTATATGCAAATGGAGATATTTAT TACCCAAGACCCGTTGCTTTTTCAATGGATTTCAGGATTTGTGCTGGCTGCAATATGGAGATTGGC CATGGAAGATTTCTGAATTGCCTTAACTCACTATGGCATCCAGAATGTTTTCGATGTTATGGCTGT AGTCACCCGATTTCTGAGTACGAGTTTTCAACATCTGGGAACTACCCTTTTCACAAGGCTTGTTAC AGGGAGAGATATCATCCAAAATGTGATGTCTGCAACCACTTTATTCCAACAAATCCTGCTGGTCTT ATTGAATATAGGGCACACCCATTTTGGATCCAGAAGTATTGCCCTTCTCACGAACACGATTCCACC CCAAGATGTTGCAGTTGTGAAAGAATGGAGCCACGGAATACGGGATATATTGAGCTTAATGATGGA CGTAAACTTTGCCTCGAGTGTCTGGACTCAGCGGTCATGGACACAAGTCAATGTCAACCTTTGTAC TTGCAGATACAAGATTTCTATGAAGGGCCTCGTCAATGCGAAGTTACTGCCATTCTCATCTTATTT GGGCTCCCTAGGCTACTGACTGGTTCGATTCTAGCTCACGAGATGATGCACGCGTGGATGCGGCTG AAAGGATTCCGGACACTGAGCCAAGACATTGAAGAAGGTATATGTCAAGTGATGGCTCATAAGTGG ATAGAAGCCGAGTTAGCTGCTGGTTCAAGAAACAGCAGTGCTGCATCATCATCATCATCGTCATCA TCTTCTTCTTCTACTAGAGGACTGAAGAAGGGACCAAAATCGCAGTACGAGAGGAAGCTTGGCGAG TTTTTCAAGCACCAGATCGAGTCTGATGCGTCTCCGGTTTATGGAGACGGGTTCAGGGCAGGGCGG CTAGCGGTTAACAAGTATGGTTTGCGAAAAACGCTTGAGCATATACAGATGACCGGTAGATTCCCG GTTTAA darl ( -857bp between PS3 and PS4 ) ( SEQ ID NO : 113 ) :

[0987] ATGGGGTGGCTTACTAAAATCCTTAAAGGCTCAAGCCATAAATTCTCGGATGGACAAGTTAACAGA AGATATAGAGATGATAGGAACTTGGAAAGTTCTCGTCTTTCTGCGGATGGATCTGATTTTGACAAA GAAGAGATTGAATGTGCTATTGCACTCTCTCTTTCAGAACAAGAACATGTAATTCCACAAGATGAC AAAGGAAAACAAGTAATCGAATACAAATCTGAAACCGAAGAAGATGAGGATGAGGATGAGGACGAA GATGAAGAATACACGAGAGCTCAGCTAGAAGCAGCGGAAGAAGAGGAAAGACGGGCAGCTCAAGCT CAAATCGAGGAAGAAGAGAAACGGCGAGCTGAAGCTGAATTAGAAGAAATAGAGAAACAGCTTGCG AAAGCTCGACTAGAAGAGGAAGAAGTTAGGCGTACCAAAGCGCAATTGGAGGAAGACGAGCAACTA GCTAAGGCCATTCAAGAAAGTATGGTTGTGGGATCTCCTCCTCGGTATGATTCTGGAAGTATGTTT CAACCATTCCCTTTCCTTAATCCATCTAGTCACAGGGGTTGCCTAGCTGAGATTGGACATGGAAGG TTTCTGAGTTGCATGGGTGGCGTTTGGCATCCTGAATGTTTCTGCTGCCATGCTTGTGATAAGCCC AT C AT AGAT T AT GAGT T C T C AAT GT C CGGG AAT TGGGGCAGGC T AC AAAT T GAT AGAC AT GAT C AC AGAGCCTTGCAGGCTGATACGTCGTTGTGAAGTCACTGCAATTCTCATTCTATATGGACTTCCTCG TTTGTTAACTGGATCAATCCTTGCTCATGAGATGATGCATGCCTGGCTTCGACTAAATGGtaaGTA TCCGAATCTTAGGCCAGAAGTTGAAGAAGGAATATGTCAGGTTTTGGCTCACATGTGGTTGGAATC TGAGACTTATGCTGGCTCTACATTAGTAGATATCGCATCTTCTTCATCATCATCATCATCGGCTGT GGTCACTGCATCGTCAAAGAAAGGTGAGAGGTCTGACTTTGAGAAGAAACTTGGTGAGTTTTTCAA GCACCAAATAGAGTCGGATTCTTCGTCGGCATATGGGGATGGGTTCAGGCAAGGTAACCAAGCAGT TCTTACGCACGGTCTGAGGCGAACCCTCGACCATATTCGCTTGACCGGTACATTTCCTTAA upl3 ( -T in PS I , - 781bp between PS 2 and PS4 ) ( SEQ ID NO : 115 ) :

[0988] ATGGAAACTCGGAGCCGCAAGCGGGCGGAGGCGACCTCAACTGCCCCATCTTCTTCTTCTTCTTCA CCTCCTCCTCCCTCAGGCCCCACCACCCGCAGCAAACGCGCTCGTCTTTCCTCTTCCTCTTCCTCC TCTGTTGCCGCTACCACCACTGCACCTTCCTCTTCCACCCGCTCTCGCTCTTCTCGCTCCGCCGCC GCCGCTGCTACTCCCATGGACACCTCCACCGAGTCTTCTGGATTTCGCCGAGGCGGACGAGGTAAC AGGGGTAACGACAACAGTAATTCTGACAAGGGCAAGGAGAAGGAGCACGACGTTAGGATTAGGGAT AGAGAAAGAGAAAGAGACCGAGCCAGAGAACAACTCAACATGGATGCTGCCGCTGCTGCTGCCGCC GCTGACGACGACGACGACAATGACAGCGAGGATGGCAACGGGGGTTTCATGCATCCCAACATGAGC TCCGCCAGCAGTGCTTTACAAGGTTTGCTGAGGAAGCTTGGAGCTGGCCTCGATGACTTGCTTCCT TCCTCTGGTATCGGCTCTGCTTCTTCTTCCCACTTAAATGGGAGGATGAAGAAGATACTCTCTGGC TTGCGCTCCGAAGGAGAAGAGGGAAAGCAGGTCGAGGCTTTAACCCAGCTATGCGAGATGCTATCC ATTGGGACTGAAGACTCATTAAGCACCTTCTCTGTTGATTCCTTCGTCCCTGTTCTCGTTGGTCTA CTAAACCATGAAAGCAATCCCGACATAATGCTTCTTGCGGCCAGGGCTCTTACCCATCTATGTGAT GTTTTGCCGTCTACACGGGGTTAATCCGATTACTTTCCACCTGTGCGAGCGGCTCACCTCTTGGAT TCAGGACATTGCTTCTTCTCGGCATTAGTAGCATTCTTAAGGATATTCTGTCGGGTCCTGGTGTCT CTGCTAATGCATCTGTATCTCCAGCACTGAGCAGGCCCGCAGATCAGATCTTTGAGATAGTCAACC TAGCGAATGAGCTCCTCCCTCCATTGCCAGAAGGAGTTATCTCCCTTCCTACTAGCACAAACACTT TGGTGAAAGGTTCAGGCCAAAAGAAATCTTCTCCAAGTACTTCAGGAAAACAAGACGATTCACCAA AAGTTTCACCTAGAGAAAAATTACTTGCTGATCAACCTGAACTTCTGCAGAAATTTGGACTGGATC TTCTTCCAGTTTTAGTGCAGATTTATGGTTCTAGTGTCAATGGTACTATTCGCCATAAATGTCTGT CAGTTATCGGAAAGTTGATGTATTTCAGCACTTCAGAAATGATTCAATCTCTAATTGGTGACACAA ATATATCGAGCTTCTTGGCTGGTGTATTGGCATGGAAAGATCCACAAGTCTTGGTTCCTGCTCTAC AAGTTGCAGAAATTCTGATGGAAAAGCTTCCTGAAACATTCTCGAAAGTGTTTGTGAGGGAAGGGG TGGTTCATGCTGTAGATCAACTTGTCTTGGTTGGTAAACCTATTTCTCATGCTTCACCTACTGATA AGGAAAATGACTGTGTGCCAGGATCTGCACGATCTAGGCGATATAGACGGCGAAGTAGTAATGCCA GTTCTGATGGAAATCAGTCAGAAGAGCTTAAGAATTCTGCGTCGGTTAACGTAGGTGCAAACCATA ATTCCCTAGATTCTCCTACCTCTAGCTTCTTGCTAAAGGAAACAGTTAGCTCCTGTGCCAAAGCAT TCAAAGACAAGTACTTCCCGTCTGATGGCGGGGATCTCGATGTTGGAGTTACAGATGATCTCTTAC ATCTAAAGAATCTTTGCACGAAGCTAACTGCTAGTATAGATGAGCATAAAGTGAAAGGAAAGGGAA AATCCAAAGCCTCTGGGCCATGCCTTGGCGATTTTTCTGCTAGCAAGGAAGAATACTTGATTGGTG TCATTTCTGAGATACTTGGCGAGCTTAGCAAAGGGGATGGAGTCTCAACTTTTGAGTTTATTGGCA GTGGTGTGGTTGCAGCTTTGCTTGACTATTTCTCTTGTGGGTACTTTTCCAAAGAGAAGATCTCCG AGGTTAATTTGCCCAAACTTCGCCAAGATGGGCTCAGAAGATTCAAAGCTTTTCTAGAAGTCGCAC TTCCGTCTGATGGTAATGAGGGAAAGATCCCTCCTATGACAGTTTTGATTCAGAAACTTCAAGATG CTTTATCTTCACTGGAACGCTTTCCCGTCGTCCTTAGTCATCCCTCAAGGTCATTAGGTGGAAGTG CTCGTCTCTCATCTGGATTGAGTGCTTTGGCACATCCTTTAAAGTTGCGATTATGCCGTGCACCTG GAGAGAAGACACTACGTGATTACTCCTCCAATATTGTTCTTATAGATCCACTGGCAAGCATTGCAG CGGTAGAAGAATTTCTTTGGCCCCGAGTTCAACGGAGTGAATCTGGTATGAAGCATGCAGCGCCTG CTGGAAATACAGAGCCAGGCACGTTACCTAGCGGTGCTGGTGTTTCATCACCATCCTCGTCAACTC CGGCTTCCACCACACGTCGTCATTCTTCTAGATCTAGATCAGCAATTAACATAGGTGATACCTCAA AGAAAGAAGCTGTGCATGAGAAAGGTACCAGCTCATCTAAAGGAAAAGGTAAAGGCGTTATGAAGC CCGTTCCAGCTGATAAGGGGCCTCAAACAAGGAGCAGTGCTCAAAGAAGAGCTGTCCTTGACAAAG ATACACATATGAAACCAGCTAGCGGAGACTCCAGCTCCGAGGATGAGGAATTGGAAATAGCCCCTG T C GAG AT T GAT GAT GCCTTGGT GAT T GAAG AGGAT GAC ATGT C TG AT GAT G AAGAT GAT GAT AAT G ACGATGTGTTGGATGACAGTCTTCCCATGTGCACGCCTGATAAAGTGCATGATGTAAAATTGGGGG ACGCAGTGGATGATGACGGTGGTGCTGGTCTGGCCTCTAGCGGGCGACAGATGAATCCAGCTTCAG GTGGCACTAGTGGAGCAGCAGCAGCGAGGGGATCTGATTCTATTGATGCTGGCATTGGGAATTCTT ACGGTTCTAGGGGTGCACTCTCCTTTGCTGCTGCGGCAATGGCTGGGCTTGGAGCTGCCAGTGGTA GAGGTATCAGGGGAAGTAGAGACCTGCATGGGCGTACCCTATATCGAAGTTCCGACGAGCCCTCTA AGTTATTATTTACTGCAGGAGGAAAGCAACTTAGTAGGCATTTGACGATATATCAGGCTGTGCAGC GACAACTTATGCTAGATGAAGACGATGATGAAAGGTTCGGTGGCAGCGATTTCATGTCAAGTGATG GAAGCAGATTCAATGATATTTACACCATCATGTACCAGAGGCCGGAGAGTCAAGCGAATAGGTTGT CTGTTGGTGCAGCAAGTTCTACCACACTATCAAAATCCACTAAATCTGCTACTGCCAATTCTAGCG TAGAATCTCAGTCATATAAGGCATCTCTTTTGGATAGTATCTTACAAGGGGAGCTTCCGTGCGATC TTGAGAAGTCAAATTCTACTTATAATGTTCTGGCGCTGTTGCGTGTATTAGAGGGGTTAAATCAGC TTGGCCCTCGGTTGAGAGCCCAAACTGTTTCTGATCGTTTCGCAGAGGGTAAAATTACAAGTCTAG ATGATCTGAAGACAACTGCTGCTGCTAAGATTTCTCATGAAGAATTCATCAATAGCAAACTTACAC CCAAATTAGCTCGACAGATCCAGGATGCGCTTGCTTTGTGCAGTGGTAGTCTTCCCTCTTGGTGCT AC C AGT T GAC T AGAGC AT GCCCGTTTTTGTTTCCGTTT C AG AC CC GG AG AC AGT AT T T C T AT T C AA CTGCTTTTGGGTTGTCCCGCGCATTGAACCGCTTGCAGCAGCAGCAAGGTGCTGACGGCAGTGGGT CTACAAATGAACGAGAGATGAGAATAGGGAGGTTGCAGCGCCAGAAAGTCCGTGTATCCCGAAATA GAATATTAGATTCTGCTGCGAAAGTTATGGAGATGTATTCTAGCCAAAAAGCTGTGCTTGAAGTAG AATATTTTGGTGAAGTTGGTACTGGTCTAGGCCCTACACTTGAGTTTTACACACTCCTAAGCCATG ATCTACAAAAGGTTGCGCTTGGGATGTGGAGATCAAATTCTGGTGACATGGTATCTATGCAAATTG

[0989] ATAGAGGTGAGATTGAAGACGGGAAATCATCCGCAGCTAGGGACAGAGATATAGTTCAGGCACCGC

[0990] TTGGGTTGTTTCCTCGACCTTGGCCCTCTACAGCTGACGTATCTGAAGGTAGTCGGTTTCATAAAG

[0991] TCATTGAATATTTCCGGCTTTTAGGGCGTGTGATGGCAAAAGCACTTCAAGATGGACGGCTACTGG

[0992] ACGTCCCATTGAGTACAGCGTTTTATAAGCTTATTCTTGGTCAAGAGCTTGATTTGCATGATATCA

[0993] TATTATTTGATACCGAGCTTGGCAAGACTTTGCAAGAGTTTCGTGTTCTTGTTGGCCGCAAGCACT

[0994] ATCTGGAGGCAGTAGGCGGTGACAATTGCAGCGCTGGTTCTGATTTATGTTTACGTGGATCCCGTA

[0995] TAGAAGATCTCTGCTTGGACTTCACGCTTCCTGGCTATCCTGAATACATTCTGAGACCAGGAGATG

[0996] AAATTGTTGATATGAATAGTCTTGAAGAGTATATATCCCTTGTCGTTGATGCTACTGTCAAGAGAG

[0997] GAGTTACCCGGCAGATTGAAGCCTTTAGATCTGGATTCAATCAGGTGTTTGACGTAACATCTCTAC

[0998] AAATATTCACCCCTTCCGAGCTGGACTATTTGCTGTGTGGTCGTAGAGAGTTGTGGGAGGCGGAGA

[0999] CTCTTGCTGAACATATCAAGTTTGATCACGGTTATACTGCTAAAAGTCCGGCAATCATTAATTTAC

[1000] TGGAGATCATGGGAGAACTTACAGCGGATCAGCAGAGGGCTTTCTGCCAATTTGTAACTGGAGCTC

[1001] CTAGGCTTCCTCCTGGTGGGCTAGCTGTTTTGAATCCAAAGCTGACGATTGTGAGAAAGCATTCAT

[1002] CAACCTCAAGTGCGGCAGCGAACGGAACAGGGGCTTCGGAGACGGCAGACGATGATCTTCCCAGCG

[1003] TAATGACTTGCGCCAACTATCTTAAGCTCCCTCCTTATTCGACAAAGGAAATCATGTACAAGAAAC

[1004] TGCTCTACGCCATCAACGAAGGGCAAGGATCGTTCGACCTGTCATAA

[1005] DA1DAR1UPL3 triple mutant protein sequences :

[1006] DAI ( SEQ ID NO : 112 ) :

[1007] MGWFNKIFKGSNQRLRVGNEHNHNGYYENYPNAHASHDEPSADTDADRDEPQTQESSTSEDTSNDQ

[1008] ENEDIDRAIALSLLEENQGQKNTGGKYVMVDEDEQLARAIQESMIVGNSPRHKSGSTYDHGNAYGT

[1009] ADLFGNGHMHGGGNVYANGDIYYPRPVAFSMDFRICAGCNMEIGHGRFLNCLNSLWHPECFRCYGC

[1010] SHPISEYEFSTSGNYPFHKACYRERYHPKCDVCNHFIPTNPAGLIEYRAHPFWIQKYCPSHEHDST

[1011] PRCCSCERMEPRNTGYIELNDGRKLCLECLDSAVMDTSQCQPLYLQIQDFYEGPRQCEVTAILILF

[1012] GLPRLLTGSILAHEMMHAWMRLKGFRTLSQDIEEGICQVMAHKWIEAELAAGSRNSSAASSSSSSS

[1013] SSSSTRGLKKGPKSQYERKLGEFFKHQIESDASPVYGDGFRAGRLAVNKYGLRKTLEHIQMTGRFP V

[1014] DARI ( SEQ ID NO : 114 ) :

[1015] MGWLTKILKGSSHKFSDGQVNRRYRDDRNLESSRLSADGSDFDKEEIECAIALSLSEQEHVIPQDD

[1016] KGKQVIEYKSETEEDEDEDEDEDEEYTRAQLEAAEEEERRAAQAQIEEEEKRRAEAELEEIEKQLA

[1017] KARLEEEEVRRTKAQLEEDEQLAKAIQESMVVGSPPRYDSGSMFQPFPFLNPSSHRGCLAEIGHGR FLSCMGGVWHPECFCCHACDKPIIDYEFSMSGN

[1018] UPL3 ( SEQ ID NO : 116 ) :

[1019] METRSRKRAEATSTAPSSSSSSPPPPSGPTTRSKRARLSSSSSSSVAATTTAPSSSTRSRSSRSAA

[1020] AAATPMDTSTESSGFRRGGRGNRGNDNSNSDKGKEKEHDVRIRDRERERDRAREQLNMDAAAAAAA ADDDDDNDSEDGNGGFMHPNMSSASSAYKVC da2-l nucleic acid sequence ( +T in PSI , +A in PS2 ) ( SEQ ID NO : 31 ) : ATGGGTAATAAGTTGGGAAGGAAGAGGCAAGTGGTGGAAGAAAGGTATACAAAGCCACAAGGGTTG

[1021] TATGTGAATAAAGATGTCGACGTTAAAAAGCTTAGGAAACTGATTGTAGAGTCAAAGCTTGCTCCT

[1022] TGCTATTCCTGGGGACGATGAAAGCTGCCACGACCTTGAAGAATGCCCGATTTGCTTCCTGTACTA

[1023] TCCTAGCCTCAATAGATCAAGATGTTGCATGAAAAGCATTTGTACAGAGTGTTTTTTGCAAATGAA

[1024] GAATCCTAACTCAGCTCGGCCTACTCAGTGCCCCTTTTGTAAAACACCTAATTATGCTGTCGAGTA

[1025] CCGTGGAGTAAAGTCAAAGGAGGAAAAGGGCATTGAACAAGTTGAAGAGCAACGGGTTATAGAAGC

[1026] CAAAATAAGGATGAGGCAGAAAGAAATGCAGGATGATGAAGAGAAGATGCAGAAACGTATGGAATC

[1027] ATGTTCCTCTAGCACAAGCGCAATGACTGGTGAAATGGAATACGGTTCAGCTTCAGCTATATCTTA

[1028] TAATTCTCCTATGGAAGATGCGGAAAATGATCCATCGCAGAACGTATCAGTTGTTAGACAACAACG

[1029] TTCACACCCTCGAGGAAACAGGGATGATGAGATTGACGTGGACCTTGAGGAGTTAATGGTCATGGA AGCAATATGGCTCTCCATTCAGGAAACAGGGATGCCGAGAAATAATACAGCGTGTGGGGAAATGAC ACCTTTCAAGGCAATATGTACCAGAAGATCATAGTTATGTTTCTTCGCCACCACGAGTGGCTCCAG TTGCAGAGCCGGAAACGCCGTCTTCATCATCTGGTGGGCTTGCTTGTGGAGTCGCTGCACTTGCTG AACGCCAGCAAATGGTTGGCGAATCCTCCAATCACAGTCACAATCACAACCACAACGTCAACGTTT CTTCCTACAGTATGCTTCCTGGCAATTGCGACAGTTACTACGACATAGAACAAGAGGTAGATGACA TCGACAACCATCATCATCATCATCATCATTACCATAATAACCACACGGAGATGGGAGGAGAAACAG GGAGCAATAGCTATGTAAGCTCTTACATGAACGGCCAGAGCTTCCACAACTTTCCTCCACCTCCTC CTCTGGTCATTGTTCCCGAGAGTTTTGAGGAGCAGATGATGATGGCTATGGCTGTGTCTTTAGCAG AGGTTCATGCCACGACCACGAGTGCGCCAACTGAAGTTACTTGGCAATAA

[1030] DA2- 1 protein sequence ( SEQ ID NO : 32 ) :

[1031] MGNKLGRKRQVVEERYTKPQGLYVNKDVDVKKLRKLIVESKLAPCY da2-2 nucleic acid sequence ( +86bp between PS3 and PS 4 ) ( SEQ ID NO : 33 ) :

[1032] ATGGGTAATAAGTTGGGAAGGAAGAGGCAAGTGGTGGAAGAAAGGTATACAAAGCCACAAGGGTTG TATGTGAATAAAGATGTCGACGTTAAAAAGCTTAGGAAACTGATTGTAGAGTCAAAGCTTGCTCCT TGCTATCCTGGGGACGATGAAAGCTGCCACGACCTTGAAGAATGCCCGATTTGCTTCCTGTACTAT CCTAGCCTCAATAGATCAAGATGTTGCATGAAAAGCATTTGTACAGAGTGTTTTTTGCAAATGAAG AATCCTAACTCAGCTCGGCCTACTCAGTGCCCCTTTTGTAAAACACCTAATTATGCTGTCGAGTAC CGTGGAGTAAAGTCAAAGGAGGAAAAGGGCATTGAACAAGTTGAAGAGCAACGGGTTATAGAAGCC AAAAT AAGGAT G AGGC AG AAAG AAAT GC AGGAT GAT GAAGAGAAG AT GC AG AAAC GT AT GG AAT C A TGTTCCTCTAGCACAAGCGCAATGACTGGTGAAATGGAATACGGTTCAGCTTCAGCTATATCTTAT AATTCTCCTATGGAAGATGCGGAAAATGATCCATCGCAGAACGTATCAGTTGTTAGACAACAACGT TCACACCCTCGAGGAAACAGGGATGATGAGATTGACGTGGACCTTGAGGAGTTAATGGTCATGGAA GCAATATGGCTCTCCATTCAGGAAACAGGGATGCCGAGAAATAATACAGCGTGTGGGGAAATGACA CCTTTCAGGCAATATGTACCAGAAGATCATAGTTATGTTTCTTCGCCACCACAAATGGTTGGCGAA TCCTCCAATCACAGTCACAATCACAACCACAACGTCAACGTTTCTTCCTACAGTATGCTTCCTGGC AAT TGC GAC AGT TACT AC GAC AT AGAAC AAGAGGT AGAT GAC ATC GAC AAC CAT CAT CAT CAT CAT CATCATTACCATAATAACCACACGGAGATGGGAGGAGAAACAGGGAGCAATAGCTATGTAAGCTCT TACATGAACGGCCAGAGCTTCCACAACTTTCCTCCACCTCCTCCTCTGGTCATTGTTCCCGAGAGT TTTGAGGAGCAGATGATGATGGCTATGGCTGTGTCTTTAGCAGAGGTTCATGCCACGACCACGAGT GCGCCAACTGAAGTTACTTGGCAATAA

[1033] DA2-2 protein sequence ( SEQ ID NO : 34 ) :

[1034] MGNKLGRKRQVVEERYTKPQGLYVNKDVDVKKLRKLIVESKLAPCYPGDDESCHDLEECPICFLYY PSLNRSRCCMKSICTECFLQMKNPNSARPTQCPFCKTPNYAVEYRGVKSKEEKGIEQVEEQRVIEA KIRMRQKEMQDDEEKMQKRMESCSSSTSAMTGEMEYGSASAISYNSPMEDAENDPSQNVSVVRQQR SHPRGNRDDE IDVDLEELMVMEAIWLSIQETGMPRNNTACGEMTPFRQYVPEDHSYVSSPPR . da2-3 nucleic acid sequence ( +G in PS3 , -A in PS4 ) ( SEQ ID NO : 35 ) : ATGGGTAATAAGTTGGGAAGGAAGAGGCAAGTGGTGGAAGAAAGGTATACAAAGCCACAAGGGTTG TATGTGAATAAAGATGTCGACGTTAAAAAGCTTAGGAAACTGATTGTAGAGTCAAAGCTTGCTCCT TGCTATCCTGGGGACGATGAAAGCTGCCACGACCTTGAAGAATGCCCGATTTGCTTCCTGTACTAT CCTAGCCTCAATAGATCAAGATGTTGCATGAAAAGCATTTGTACAGAGTGTTTTTTGCAAATGAAG AATCCTAACTCAGCTCGGCCTACTCAGTGCCCCTTTTGTAAAACACCTAATTATGCTGTCGAGTAC CGTGGAGTAAAGTCAAAGGAGGAAAAGGGCATTGAACAAGTTGAAGAGCAACGGGTTATAGAAGCC AAAAT AAGGAT G AGGC AG AAAG AAAT GC AGGAT GAT GAAGAGAAG AT GC AG AAAC GT AT GG AAT C A TGTTCCTCTAGCACAAGCGCAATGACTGGTGAAATGGAATACGGTTCAGCTTCAGCTATATCTTAT AATTCTCCTATGGAAGATGCGGAAAATGATCCATCGCAGAACGTATCAGTTGTTAGACAACAACGT TCACACCCTCGAGGAAACAGGGATGATGAGATTGACGTGGACCTTGAGGAGTTAATGGTCATGGAA GCAATATGGCTCTCCATTCAGGAAACAGGGATGCCGAGAAATAATACAGCGTGTGGGGAAATGACA CCTTTCAGGCAATATGTACCAGAAGATCATAGTTATGTTTCTTCGCCACCACGAGGTGGCTCCAGT TGCAGAGCCGGAAACGCCGTCTTCATCATCTGGTGGGCTTGCTTGTGGAGTCGCTGCACTTGCTGA ACGCCAGCAATGGTTGGCGAATCCTCCAATCACAGTCACAATCACAACCACAACGTCAACGTTTCT TCCTACAGTATGCTTCCTGGCAATTGCGACAGTTACTACGACATAGAACAAGAGGTAGATGACATC GACAACCATCATCATCATCATCATCATTACCATAATAACCACACGGAGATGGGAGGAGAAACAGGG AGCAATAGCTATGTAAGCTCTTACATGAACGGCCAGAGCTTCCACAACTTTCCTCCACCTCCTCCT CTGGTCATTGTTCCCGAGAGTTTTGAGGAGCAGATGATGATGGCTATGGCTGTGTCTTTAGCAGAG GTTCATGCCACGACCACGAGTGCGCCAACTGAAGTTACTTGGCAATAA

[1035] DA2-3 protein sequence ( SEQ ID NO : 36 ) :

[1036] MGNKLGRKRQVVEERYTKPQGLYVNKDVDVKKLRKLIVESKLAPCYPGDDESCHDLEECPICFLYY PSLNRSRCCMKSICTECFLQMKNPNSARPTQCPFCKTPNYAVEYRGVKSKEEKGIEQVEEQRVIEA KIRMRQKEMQDDEEKMQKRMESCSSSTSAMTGEMEYGSASAISYNSPMEDAENDPSQNVSVVRQQR SHPRGNRDDE IDVDLEELMVMEAIWLSIQETGMPRNNTACGEMTPFRQYVPEDHSYVSSPPRGGSS CRAGNAVFI I WWACLWSRCTC

[1037] ( da2da l ) -l double mutant nucleic acid sequences : da2 ( -T in PSI , -7bp in PS2 ; stacked in da l -1 ) ( SEQ ID NO : 117 ) :

[1038] ATGGGTAATAAGTTGGGAAGGAAGAGGCAAGTGGTGGAAGAAAGGTATACAAAGCCACAAGGGTTG TATGTGAATAAAGATGTCGACGTTAAAAAGCTTAGGAAACTGATTGTAGAGTCAAAGCTTGCTCCT TGCATCCTGGGGACGATGAAAGCTGCCACGACCTTGAAGAATGCCCGATTTGCTTCCTGTACTATC CTAGCCTCAATAGATCAAGATGTTGCATGAAAAGCATTTGTACAGAGTGTTTTTTGCAAATGAAGA ATCCTAACTCAGCTCGGCCTACTCAGTGCCCCTTTTGTAAAACACCTAATTATGCTGTCGAGTACC GTGGAGTAAAGTCAAAGGAGGAAAAGGGCATTGAACAAGTTGAAGAGCAACGGGTTATAGAAGCCA AAATAAGGATGAGGCAGAAAGAAATGCAGGATGATGAAGAGAAGATGCAGAAACGTATGGAATCAT GTTCCTCTAGCACAAGCGCAATGACTGGTGAAATGGAATACGGTTCAGCTTCAGCTATATCTTATA ATTCTCCTATGGAAGATGCGGAAAATGATCCATCGCAGAACGTATCAGTTGTTAGACAACAACGTT CACACCCTCGAGGAAACAGGGATGATGAGATTGACGTGGACCTTGAGGAGTTAATGGTCATGGAAG C AAT AT GGCT CT CC AT TC AGGAAACAGGGATGCCGAGAAAT AATACAGCGT GT GGGGAAAT GACAC CTAATATGTACCAGAAGATCATAGTTATGTTTCTTCGCCACCACGAGTGGCTCCAGTTGCAGAGCC GGAAACGCCGTCTTCATCATCTGGTGGGCTTGCTTGTGGAGTCGCTGCACTTGCTGAACGCCAGCA AATGGTTGGCGAATCCTCCAATCACAGTCACAATCACAACCACAACGTCAACGTTTCTTCCTACAG TATGCTTCCTGGCAATTGCGACAGTTACTACGACATAGAACAAGAGGTAGATGACATCGACAACCA TCATCATCATCATCATCATTACCATAATAACCACACGGAGATGGGAGGAGAAACAGGGAGCAATAG CTATGTAAGCTCTTACATGAACGGCCAGAGCTTCCACAACTTTCCTCCACCTCCTCCTCTGGTCAT TGTTCCCGAGAGTTTTGAGGAGCAGATGATGATGGCTATGGCTGTGTCTTTAGCAGAGGTTCATGC CACGACCACGAGTGCGCCAACTGAAGTTACTTGGCAATAA

[1039] ( da2 dal ) -l double mutant protein sequence :

[1040] DA2 ( SEQ ID NO : 118 ) :

[1041] MGNKLGRKRQVVEERYTKPQGLYVNKDVDVKKLRKLIVESKLAPCILGTMKAATTLKNARFASCTI LASIDQDVA

[1042] ( da2da l ) -2 double mutant nucleic acid sequences : da2 ( +T in PSI , +A in PS2 ; stacked in dal -1 ) ( SEQ ID NO : 119 ) :

[1043] ATGGGTAATAAGTTGGGAAGGAAGAGGCAAGTGGTGGAAGAAAGGTATACAAAGCCACAAGGGTTG TATGTGAATAAAGATGTCGACGTTAAAAAGCTTAGGAAACTGATTGTAGAGTCAAAGCTTGCTCCT TGCTTATCCTGGGGACGATGAAAGCTGCCACGACCTTGAAGAATGCCCGATTTGCTTCCTGTACTA TCCTAGCCTCAATAGATCAAGATGTTGCATGAAAAGCATTTGTACAGAGTGTTTTTTGCAAATGAA GAATCCTAACTCAGCTCGGCCTACTCAGTGCCCCTTTTGTAAAACACCTAATTATGCTGTCGAGTA CCGTGGAGTAAAGTCAAAGGAGGAAAAGGGCATTGAACAAGTTGAAGAGCAACGGGTTATAGAAGC

[1044] CAAAATAAGGATGAGGCAGAAAGAAATGCAGGATGATGAAGAGAAGATGCAGAAACGTATGGAATC

[1045] ATGTTCCTCTAGCACAAGCGCAATGACTGGTGAAATGGAATACGGTTCAGCTTCAGCTATATCTTA

[1046] TAATTCTCCTATGGAAGATGCGGAAAATGATCCATCGCAGAACGTATCAGTTGTTAGACAACAACG

[1047] TTCACACCCTCGAGGAAACAGGGATGATGAGATTGACGTGGACCTTGAGGAGTTAATGGTCATGGA

[1048] AGCAATATGGCTCTCCATTCAGGAAACAGGGATGCCGAGAAATAATACAGCGTGTGGGGAAATGAC

[1049] ACCTTTCAAGGCAATATGTACCAGAAGATCATAGTTATGTTTCTTCGCCACCACGAGTGGCTCCAG

[1050] TTGCAGAGCCGGAAACGCCGTCTTCATCATCTGGTGGGCTTGCTTGTGGAGTCGCTGCACTTGCTG

[1051] AACGCCAGCAAATGGTTGGCGAATCCTCCAATCACAGTCACAATCACAACCACAACGTCAACGTTT

[1052] CTTCCTACAGTATGCTTCCTGGCAATTGCGACAGTTACTACGACATAGAACAAGAGGTAGATGACA

[1053] TCGACAACCATCATCATCATCATCATCATTACCATAATAACCACACGGAGATGGGAGGAGAAACAG

[1054] GGAGCAATAGCTATGTAAGCTCTTACATGAACGGCCAGAGCTTCCACAACTTTCCTCCACCTCCTC

[1055] CTCTGGTCATTGTTCCCGAGAGTTTTGAGGAGCAGATGATGATGGCTATGGCTGTGTCTTTAGCAG

[1056] AGGTTCATGCCACGACCACGAGTGCGCCAACTGAAGTTACTTGGCAATAA

[1057] ( da2dal ) -2 double mutant protein sequences :

[1058] DA2 ( SEQ ID NO : 120 ) :

[1059] MGNKLGRKRQVVEERYTKPQGLYVNKDVDVKKLRKLIVESKLAPCLSWGR

[1060] ( da2darl ) -1 double mutant nucleic acid sequences : da2 ( +T in PSI ; stacked in darl -1 ) ( SEQ ID NO : 121 ) :

[1061] ATGGGTAATAAGTTGGGAAGGAAGAGGCAAGTGGTGGAAGAAAGGTATACAAAGCCACAAGGGTTG

[1062] TATGTGAATAAAGATGTCGACGTTAAAAAGCTTAGGAAACTGATTGTAGAGTCAAAGCTTGCTCCT

[1063] TGCTTATCCTGGGGACGATGAAAGCTGCCACGACCTTGAAGAATGCCCGATTTGCTTCCTGTACTA

[1064] TCCTAGCCTCAATAGATCAAGATGTTGCATGAAAAGCATTTGTACAGAGTGTTTTTTGCAAATGAA

[1065] GAATCCTAACTCAGCTCGGCCTACTCAGTGCCCCTTTTGTAAAACACCTAATTATGCTGTCGAGTA

[1066] CCGTGGAGTAAAGTCAAAGGAGGAAAAGGGCATTGAACAAGTTGAAGAGCAACGGGTTATAGAAGC

[1067] CAAAATAAGGATGAGGCAGAAAGAAATGCAGGATGATGAAGAGAAGATGCAGAAACGTATGGAATC

[1068] ATGTTCCTCTAGCACAAGCGCAATGACTGGTGAAATGGAATACGGTTCAGCTTCAGCTATATCTTA

[1069] TAATTCTCCTATGGAAGATGCGGAAAATGATCCATCGCAGAACGTATCAGTTGTTAGACAACAACG

[1070] TTCACACCCTCGAGGAAACAGGGATGATGAGATTGACGTGGACCTTGAGGAGTTAATGGTCATGGA

[1071] AGCAATATGGCTCTCCATTCAGGAAACAGGGATGCCGAGAAATAATACAGCGTGTGGGGAAATGAC

[1072] ACCTTTCAGGCAATATGTACCAGAAGATCATAGTTATGTTTCTTCGCCACCACGAGTGGCTCCAGT

[1073] TGCAGAGCCGGAAACGCCGTCTTCATCATCTGGTGGGCTTGCTTGTGGAGTCGCTGCACTTGCTGA

[1074] ACGCCAGCAAATGGTTGGCGAATCCTCCAATCACAGTCACAATCACAACCACAACGTCAACGTTTC

[1075] TTCCTACAGTATGCTTCCTGGCAATTGCGACAGTTACTACGACATAGAACAAGAGGTAGATGACAT

[1076] CGACAACCATCATCATCATCATCATCATTACCATAATAACCACACGGAGATGGGAGGAGAAACAGG

[1077] GAGCAATAGCTATGTAAGCTCTTACATGAACGGCCAGAGCTTCCACAACTTTCCTCCACCTCCTCC

[1078] TCTGGTCATTGTTCCCGAGAGTTTTGAGGAGCAGATGATGATGGCTATGGCTGTGTCTTTAGCAGA

[1079] GGTTCATGCCACGACCACGAGTGCGCCAACTGAAGTTACTTGGCAATAA

[1080] ( da2darl ) -l double mutant protein sequence :

[1081] DA2 ( SEQ ID NO : 122 ) :

[1082] MGNKLGRKRQVVEERYTKPQGLYVNKDVDVKKLRKLIVESKLAPCLSWGR

[1083] ( da2da ldarl ) -1 triple mutant nucleic acid sequences : da2 ( IT in PSI , +A in PS2 ; stacked in ( daldarl) -1 ) ( SEQ ID

[1084] NO : 123 ) :

[1085] ATGGGTAATAAGTTGGGAAGGAAGAGGCAAGTGGTGGAAGAAAGGTATACAAAGCCACAAGGGTTG

[1086] TATGTGAATAAAGATGTCGACGTTAAAAAGCTTAGGAAACTGATTGTAGAGTCAAAGCTTGCTCCT

[1087] TGCTTATCCTGGGGACGATGAAAGCTGCCACGACCTTGAAGAATGCCCGATTTGCTTCCTGTACTA TCCTAGCCTCAATAGATCAAGATGTTGCATGAAAAGCATTTGTACAGAGTGTTTTTTGCAAATGAA GAATCCTAACTCAGCTCGGCCTACTCAGTGCCCCTTTTGTAAAACACCTAATTATGCTGTCGAGTA CCGTGGAGTAAAGTCAAAGGAGGAAAAGGGCATTGAACAAGTTGAAGAGCAACGGGTTATAGAAGC CAAAATAAGGATGAGGCAGAAAGAAATGCAGGATGATGAAGAGAAGATGCAGAAACGTATGGAATC ATGTTCCTCTAGCACAAGCGCAATGACTGGTGAAATGGAATACGGTTCAGCTTCAGCTATATCTTA TAATTCTCCTATGGAAGATGCGGAAAATGATCCATCGCAGAACGTATCAGTTGTTAGACAACAACG TTCACACCCTCGAGGAAACAGGGATGATGAGATTGACGTGGACCTTGAGGAGTTAATGGTCATGGA AGCAATATGGCTCTCCATTCAGGAAACAGGGATGCCGAGAAATAATACAGCGTGTGGGGAAATGAC ACCTTTCAAGGCAATATGTACCAGAAGATCATAGTTATGTTTCTTCGCCACCACGAGTGGCTCCAG TTGCAGAGCCGGAAACGCCGTCTTCATCATCTGGTGGGCTTGCTTGTGGAGTCGCTGCACTTGCTG AACGCCAGCAAATGGTTGGCGAATCCTCCAATCACAGTCACAATCACAACCACAACGTCAACGTTT CTTCCTACAGTATGCTTCCTGGCAATTGCGACAGTTACTACGACATAGAACAAGAGGTAGATGACA TCGACAACCATCATCATCATCATCATCATTACCATAATAACCACACGGAGATGGGAGGAGAAACAG GGAGCAATAGCTATGTAAGCTCTTACATGAACGGCCAGAGCTTCCACAACTTTCCTCCACCTCCTC CTCTGGTCATTGTTCCCGAGAGTTTTGAGGAGCAGATGATGATGGCTATGGCTGTGTCTTTAGCAG AGGTTCATGCCACGACCACGAGTGCGCCAACTGAAGTTACTTGGCAATAA

[1088] ( da2 daldarl ) -1 triple mutant protein sequences :

[1089] DA2 ( SEQ ID NO : 124 ) :

[1090] MGNKLGRKRQVVEERYTKPQGLYVNKDVDVKKLRKLIVESKLAPCLSWGR

[1091] ( da2tt8fael ) -1 triple mutant nucleic acid sequences : da2 ( - 85bp between PS3 and PS4 ; stacked in tt8fael ) ( SEQ ID

[1092] NO : 125 ) :

[1093] ATGGGTAATAAGTTGGGAAGGAAGAGGCAAGTGGTGGAAGAAAGGTATACAAAGCCACAAGGGTTG TATGTGAATAAAGATGTCGACGTTAAAAAGCTTAGGAAACTGATTGTAGAGTCAAAGCTTGCTCCT TGCTATCCTGGGGACGATGAAAGCTGCCACGACCTTGAAGAATGCCCGATTTGCTTCCTGTACTAT CCTAGCCTCAATAGATCAAGATGTTGCATGAAAAGCATTTGTACAGAGTGTTTTTTGCAAATGAAG AATCCTAACTCAGCTCGGCCTACTCAGTGCCCCTTTTGTAAAACACCTAATTATGCTGTCGAGTAC CGTGGAGTAAAGTCAAAGGAGGAAAAGGGCATTGAACAAGTTGAAGAGCAACGGGTTATAGAAGCC AAAAT AAGGAT G AGGC AG AAAG AAAT GC AGGAT GAT GAAGAGAAG AT GC AG AAAC GT AT GG AAT C A TGTTCCTCTAGCACAAGCGCAATGACTGGTGAAATGGAATACGGTTCAGCTTCAGCTATATCTTAT AATTCTCCTATGGAAGATGCGGAAAATGATCCATCGCAGAACGTATCAGTTGTTAGACAACAACGT TCACACCCTCGAGGAAACAGGGATGATGAGATTGACGTGGACCTTGAGGAGTTAATGGTCATGGAA GCAATATGGCTCTCCATTCAGGAAACAGGGATGCCGAGAAATAATACAGCGTGTGGGGAAATGACA CCTTTCAGGCAATATGTACCAGAAGATCATAGTTATGTTTCTTCGCCACCACGAGAATGGTTGGCG AATCCTCCAATCACAGTCACAATCACAACCACAACGTCAACGTTTCTTCCTACAGTATGCTTCCTG GC AAT TGC GAC AGT T ACT ACGAC AT AGAAC AAGAGGT AG AT GAC AT C GAC AAC CAT CAT CAT CATC ATCATCATTACCATAATAACCACACGGAGATGGGAGGAGAAACAGGGAGCAATAGCTATGTAAGCT CTTACATGAACGGCCAGAGCTTCCACAACTTTCCTCCACCTCCTCCTCTGGTCATTGTTCCCGAGA GTTTTGAGGAGCAGATGATGATGGCTATGGCTGTGTCTTTAGCAGAGGTTCATGCCACGACCACGA GTGCGCCAACTGAAGTTACTTGGCAATAA

[1094] ( da2 tt 8f ae l ) -1 triple mutant protein sequences :

[1095] DA2 ( SEQ ID NO : 126 ) :

[1096] MGNKLGRKRQVVEERYTKPQGLYVNKDVDVKKLRKLIVESKLAPCYPGDDESCHDLEECPICFLYY PSLNRSRCCMKSICTECFLQMKNPNSARPTQCPFCKTPNYAVEYRGVKSKEEKGIEQVEEQRVIEA KIRMRQKEMQDDEEKMQKRMESCSSSTSAMTGEMEYGSASAISYNSPMEDAENDPSQNVSVVRQQR SHPRGNRDDE IDVDLEELMVMEAIWLSIQETGMPRNNTACGEMTPFRQYVPEDHSYVSSPPRMVGE SSNHSHNHNHNVNVSSYSMLPGNCDSYYDIEQEVDDIDNHHHHHHHYHNNHTEMGGETGSNSYVSS YMNGQS FHNFPPPPPLVIVPES FEEQMMMAMAVSLAEVHATTTSAPTEVTWQ ( da2da l tt8fael ) -1 quadruple mutant nucleic acid sequences : da2 ( -3bp in PSI , G to C substitution in PS2 ; stacked in ( dal ttSfael ) -1 ) ( SEQ ID NO : 127 ) :

[1097] ATGGGTAATAAGTTGGGAAGGAAGAGGCAAGTGGTGGAAGAAAGGTATACAAAGCCACAAGGGTTG TATGTGAATAAAGATGTCGACGTTAAAAAGCTTAGGAAACTGATTGTAGAGTCAAAGCTTGCTCCT TGTCCTGGGGACGATGAAAGCTGCCACGACCTTGAAGAATGCCCGATTTGCTTCCTGTACTATCCT AGCCTCAATAGATCAAGATGTTGCATGAAAAGCATTTGTACAGAGTGTTTTTTGCAAATGAAGAAT CCTAACTCAGCTCGGCCTACTCAGTGCCCCTTTTGTAAAACACCTAATTATGCTGTCGAGTACCGT GGAGT AAAGT CAAAGGAGGAAAAGGGCATT GAAC AAGT T GAAGAGCAACGGGT T AT AGAAGCC AAA ATAAGGATGAGGCAGAAAGAAATGCAGGATGATGAAGAGAAGATGCAGAAACGTATGGAATCATGT TCCTCTAGCACAAGCGCAATGACTGGTGAAATGGAATACGGTTCAGCTTCAGCTATATCTTATAAT TCTCCTATGGAAGATGCGGAAAATGATCCATCGCAGAACGTATCAGTTGTTAGACAACAACGTTCA CACCCTCGAGGAAACAGGGATGATGAGATTGACGTGGACCTTGAGGAGTTAATGGTCATGGAAGCA ATATGGCTCTCCATTCAGGAAACAGGGATGCCGAGAAATAATACAGCGTGTGGGGAAATGACACCT TTCACGCAATATGTACCAGAAGATCATAGTTATGTTTCTTCGCCACCACGAGTGGCTCCAGTTGCA GAGCCGGAAACGCCGTCTTCATCATCTGGTGGGCTTGCTTGTGGAGTCGCTGCACTTGCTGAACGC CAGCAAATGGTTGGCGAATCCTCCAATCACAGTCACAATCACAACCACAACGTCAACGTTTCTTCC TACAGTATGCTTCCTGGCAATTGCGACAGTTACTACGACATAGAACAAGAGGTAGATGACATCGAC AACCATCATCATCATCATCATCATTACCATAATAACCACACGGAGATGGGAGGAGAAACAGGGAGC AATAGCTATGTAAGCTCTTACATGAACGGCCAGAGCTTCCACAACTTTCCTCCACCTCCTCCTCTG GTCATTGTTCCCGAGAGTTTTGAGGAGCAGATGATGATGGCTATGGCTGTGTCTTTAGCAGAGGTT CATGCCACGACCACGAGTGCGCCAACTGAAGTTACTTGGCAATAA

[1098] ( da2daltt8 fael ) -1 quadruple mutant protein sequences :

[1099] DA2 ( SEQ ID NO : 128 ) :

[1100] MGNKLGRKRQVVEERYTKPQGLYVNKDVDVKKLRKLIVESKLAPCPGDDESCHDLEECPICFLYYP SLNRSRCCMKSICTECFLQMKNPNSARPTQCPFCKTPNYAVEYRGVKSKEEKGIEQVEEQRVIEAK IRMRQKEMQDDEEKMQKRMESCSSSTSAMTGEMEYGSASAISYNSPMEDAENDPSQNVSVVRQQRS HPRGNRDDEIDVDLEELMVMEAIWLSIQETGMPRNNTACGEMTPFTQYVPEDHSYVSSPPRVAPVA EPETPSSSSGGLACGVAALAERQQMVGESSNHSHNHNHNVNVSSYSMLPGNCDSYYDIEQEVDDID NHHHHHHHYHNNHTEMGGETGSNSYVSSYMNGQSFHNFPPPPPLVIVPESFEEQMMMAMAVSLAEV HATTTSAPTEVTWQ

[1101] ( da2da 1 tt8fael ) -2 quadruple mutant nucleic acid sequences : da2 ( +T in PSI , G to C substitution in PS2 ; stacked in ( dal tt8fael ) -1 ) ( SEQ ID NO : 129 ) :

[1102] ATGGGTAATAAGTTGGGAAGGAAGAGGCAAGTGGTGGAAGAAAGGTATACAAAGCCACAAGGGTTG TATGTGAATAAAGATGTCGACGTTAAAAAGCTTAGGAAACTGATTGTAGAGTCAAAGCTTGCTCCT TGCTTATCCTGGGGACGATGAAAGCTGCCACGACCTTGAAGAATGCCCGATTTGCTTCCTGTACTA TCCTAGCCTCAATAGATCAAGATGTTGCATGAAAAGCATTTGTACAGAGTGTTTTTTGCAAATGAA GAATCCTAACTCAGCTCGGCCTACTCAGTGCCCCTTTTGTAAAACACCTAATTATGCTGTCGAGTA CCGTGGAGTAAAGTCAAAGGAGGAAAAGGGCATTGAACAAGTTGAAGAGCAACGGGTTATAGAAGC CAAAATAAGGATGAGGCAGAAAGAAATGCAGGATGATGAAGAGAAGATGCAGAAACGTATGGAATC ATGTTCCTCTAGCACAAGCGCAATGACTGGTGAAATGGAATACGGTTCAGCTTCAGCTATATCTTA TAATTCTCCTATGGAAGATGCGGAAAATGATCCATCGCAGAACGTATCAGTTGTTAGACAACAACG TTCACACCCTCGAGGAAACAGGGATGATGAGATTGACGTGGACCTTGAGGAGTTAATGGTCATGGA AGCAATATGGCTCTCCATTCAGGAAACAGGGATGCCGAGAAATAATACAGCGTGTGGGGAAATGAC ACCTTTCACGCAATATGTACCAGAAGATCATAGTTATGTTTCTTCGCCACCACGAGTGGCTCCAGT TGCAGAGCCGGAAACGCCGTCTTCATCATCTGGTGGGCTTGCTTGTGGAGTCGCTGCACTTGCTGA ACGCCAGCAAATGGTTGGCGAATCCTCCAATCACAGTCACAATCACAACCACAACGTCAACGTTTC TTCCTACAGTATGCTTCCTGGCAATTGCGACAGTTACTACGACATAGAACAAGAGGTAGATGACAT C G AC AACC AT CAT CAT CAT CAT CAT CAT T ACC AT AAT AACC AC AC GG AG AT GGGAGG AG AAAC AGG GAGCAATAGCTATGTAAGCTCTTACATGAACGGCCAGAGCTTCCACAACTTTCCTCCACCTCCTCC TCTGGTCATTGTTCCCGAGAGTTTTGAGGAGCAGATGATGATGGCTATGGCTGTGTCTTTAGCAGA GGTTCATGCCACGACCACGAGTGCGCCAACTGAAGTTACTTGGCAATAA

[1103] ( da2 daltt8 fael ) -2 quadruple mutant protein sequences :

[1104] DA2 ( SEQ ID NO : 130 ) :

[1105] MGNKLGRKRQVVEERYTKPQGLYVNKDVDVKKLRKLIVESKLAPCLSWGR

[1106] ( da2darl tt8fael ) -1 quadruple mutant nucleic acid sequences : da2 ( +T in PSI , -4bp in PS2 ; stacked in ( darl tt8fael ) -1 ) ( SEQ ID NO : 131 ) :

[1107] ATGGGTAATAAGTTGGGAAGGAAGAGGCAAGTGGTGGAAGAAAGGTATACAAAGCCACAAGGGTTG TATGTGAATAAAGATGTCGACGTTAAAAAGCTTAGGAAACTGATTGTAGAGTCAAAGCTTGCTCCT TGCTTATCCTGGGGACGATGAAAGCTGCCACGACCTTGAAGAATGCCCGATTTGCTTCCTGTACTA TCCTAGCCTCAATAGATCAAGATGTTGCATGAAAAGCATTTGTACAGAGTGTTTTTTGCAAATGAA GAATCCTAACTCAGCTCGGCCTACTCAGTGCCCCTTTTGTAAAACACCTAATTATGCTGTCGAGTA CCGTGGAGTAAAGTCAAAGGAGGAAAAGGGCATTGAACAAGTTGAAGAGCAACGGGTTATAGAAGC

[1108] CAAAATAAGGATGAGGCAGAAAGAAATGCAGGATGATGAAGAGAAGATGCAGAAACGTATGGAATC ATGTTCCTCTAGCACAAGCGCAATGACTGGTGAAATGGAATACGGTTCAGCTTCAGCTATATCTTA TAATTCTCCTATGGAAGATGCGGAAAATGATCCATCGCAGAACGTATCAGTTGTTAGACAACAACG TTCACACCCTCGAGGAAACAGGGATGATGAGATTGACGTGGACCTTGAGGAGTTAATGGTCATGGA AGCAATATGGCTCTCCATTCAGGAAACAGGGATGCCGAGAAATAATACAGCGTGTGGGGAAATGAC ACCTTTCAATATGTACCAGAAGATCATAGTTATGTTTCTTCGCCACCACGAGTGGCTCCAGTTGCA GAGCCGGAAACGCCGTCTTCATCATCTGGTGGGCTTGCTTGTGGAGTCGCTGCACTTGCTGAACGC CAGCAAATGGTTGGCGAATCCTCCAATCACAGTCACAATCACAACCACAACGTCAACGTTTCTTCC TACAGTATGCTTCCTGGCAATTGCGACAGTTACTACGACATAGAACAAGAGGTAGATGACATCGAC AACCATCATCATCATCATCATCATTACCATAATAACCACACGGAGATGGGAGGAGAAACAGGGAGC AATAGCTATGTAAGCTCTTACATGAACGGCCAGAGCTTCCACAACTTTCCTCCACCTCCTCCTCTG GTCATTGTTCCCGAGAGTTTTGAGGAGCAGATGATGATGGCTATGGCTGTGTCTTTAGCAGAGGTT CATGCCACGACCACGAGTGCGCCAACTGAAGTTACTTGGCAATAA

[1109] ( da2 darltt 8f ae l ) -1 quadruple mutant protein sequences :

[1110] DA2 ( SEQ ID NO : 132 ) :

[1111] MGNKLGRKRQVVEERYTKPQGLYVNKDVDVKKLRKLIVESKLAPCLSWGR

[1112] ( da2darl tt8fael ) -2 quadruple mutant nucleic acid sequences : da2 ( G to C substitution in PS2 ; stacked in ( darl tt8fael ) -1 ) ( SEQ ID NO : 133 ) :

[1113] ATGGGTAATAAGTTGGGAAGGAAGAGGCAAGTGGTGGAAGAAAGGTATACAAAGCCACAAGGGTTG TATGTGAATAAAGATGTCGACGTTAAAAAGCTTAGGAAACTGATTGTAGAGTCAAAGCTTGCTCCT TGCTATCCTGGGGACGATGAAAGCTGCCACGACCTTGAAGAATGCCCGATTTGCTTCCTGTACTAT CCTAGCCTCAATAGATCAAGATGTTGCATGAAAAGCATTTGTACAGAGTGTTTTTTGCAAATGAAG AATCCTAACTCAGCTCGGCCTACTCAGTGCCCCTTTTGTAAAACACCTAATTATGCTGTCGAGTAC CGTGGAGTAAAGTCAAAGGAGGAAAAGGGCATTGAACAAGTTGAAGAGCAACGGGTTATAGAAGCC AAAAT AAGGAT G AGGC AG AAAG AAAT GC AGGAT GAT GAAGAGAAG AT GC AG AAAC GT AT GG AAT C A

[1114] TGTTCCTCTAGCACAAGCGCAATGACTGGTGAAATGGAATACGGTTCAGCTTCAGCTATATCTTAT AATTCTCCTATGGAAGATGCGGAAAATGATCCATCGCAGAACGTATCAGTTGTTAGACAACAACGT TCACACCCTCGAGGAAACAGGGATGATGAGATTGACGTGGACCTTGAGGAGTTAATGGTCATGGAA GCAATATGGCTCTCCATTCAGGAAACAGGGATGCCGAGAAATAATACAGCGTGTGGGGAAATGACA CCTTTCACGCAATATGTACCAGAAGATCATAGTTATGTTTCTTCGCCACCACGAGTGGCTCCAGTT GCAGAGCCGGAAACGCCGTCTTCATCATCTGGTGGGCTTGCTTGTGGAGTCGCTGCACTTGCTGAA CGCCAGCAAATGGTTGGCGAATCCTCCAATCACAGTCACAATCACAACCACAACGTCAACGTTTCT TCCTACAGTATGCTTCCTGGCAATTGCGACAGTTACTACGACATAGAACAAGAGGTAGATGACATC GACAACCATCATCATCATCATCATCATTACCATAATAACCACACGGAGATGGGAGGAGAAACAGGG AGCAATAGCTATGTAAGCTCTTACATGAACGGCCAGAGCTTCCACAACTTTCCTCCACCTCCTCCT CTGGTCATTGTTCCCGAGAGTTTTGAGGAGCAGATGATGATGGCTATGGCTGTGTCTTTAGCAGAG GTTCATGCCACGACCACGAGTGCGCCAACTGAAGTTACTTGGCAATAA

[1115] ( da2 darltt 8f ae l ) -2 quadruple mutant protein sequences :

[1116] DA2 ( SEQ ID NO : 134 ) :

[1117] MGNKLGRKRQVVEERYTKPQGLYVNKDVDVKKLRKLIVESKLAPCYPGDDESCHDLEECPICFLYY PSLNRSRCCMKSICTECFLQMKNPNSARPTQCPFCKTPNYAVEYRGVKSKEEKGIEQVEEQRVIEA KIRMRQKEMQDDEEKMQKRMESCSSSTSAMTGEMEYGSASAISYNSPMEDAENDPSQNVSVVRQQR SHPRGNRDDE IDVDLEELMVMEAIWLSIQETGMPRNNTACGEMTPFTQYVPEDHSYVSSPPRVAPV AEPETPSSSSGGLACGVAALAERQQMVGESSNHSHNHNHNVNVSSYSMLPGNCDSYYDIEQEVDDI DNHHHHHHHYHNNHTEMGGETGSNSYVSSYMNGQSFHNFPPPPPLVIVPES FEEQMMMAMAVSLAE VHATTTSAPTEVTWQ

[1118] ( da2da ldarl tt8fael ') -l quintuple mutant nucleic acid sequences : da2 ( -2bp in PSI , -4bp in PS2 ; stacked in ( da ldarl t t8fael ) - 1 ) ( SEQ ID NO : 135 ) :

[1119] ATGGGTAATAAGTTGGGAAGGAAGAGGCAAGTGGTGGAAGAAAGGTATACAAAGCCACAAGGGTTG TATGTGAATAAAGATGTCGACGTTAAAAAGCTTAGGAAACTGATTGTAGAGTCAAAGCTTGCTCCT TGCTCCTGGGGACGATGAAAGCTGCCACGACCTTGAAGAATGCCCGATTTGCTTCCTGTACTATCC TAGCCTCAATAGATCAAGATGTTGCATGAAAAGCATTTGTACAGAGTGTTTTTTGCAAATGAAGAA TCCTAACTCAGCTCGGCCTACTCAGTGCCCCTTTTGTAAAACACCTAATTATGCTGTCGAGTACCG TGGAGTAAAGTCAAAGGAGGAAAAGGGCATTGAACAAGTTGAAGAGCAACGGGTTATAGAAGCCAA AAT AAGGAT G AGGC AG AAAG AAAT GC AGGAT GAT GAAG AGAAG AT GC AG AAAC GT AT GG AAT C AT G TTCCTCTAGCACAAGCGCAATGACTGGTGAAATGGAATACGGTTCAGCTTCAGCTATATCTTATAA TTCTCCTATGGAAGATGCGGAAAATGATCCATCGCAGAACGTATCAGTTGTTAGACAACAACGTTC ACACCCTCGAGGAAACAGGGATGATGAGATTGACGTGGACCTTGAGGAGTTAATGGTCATGGAAGC AATATGGCTCTCCATTCAGGAAACAGGGATGCCGAGAAATAATACAGCGTGTGGGGAAATGACACC TTTCAATATGTACCAGAAGATCATAGTTATGTTTCTTCGCCACCACGAGTGGCTCCAGTTGCAGAG CCGGAAACGCCGTCTTCATCATCTGGTGGGCTTGCTTGTGGAGTCGCTGCACTTGCTGAACGCCAG CAAATGGTTGGCGAATCCTCCAATCACAGTCACAATCACAACCACAACGTCAACGTTTCTTCCTAC AGTATGCTTCCTGGCAATTGCGACAGTTACTACGACATAGAACAAGAGGTAGATGACATCGACAAC CATCATCATCATCATCATCATTACCATAATAACCACACGGAGATGGGAGGAGAAACAGGGAGCAAT AGCTATGTAAGCTCTTACATGAACGGCCAGAGCTTCCACAACTTTCCTCCACCTCCTCCTCTGGTC ATTGTTCCCGAGAGTTTTGAGGAGCAGATGATGATGGCTATGGCTGTGTCTTTAGCAGAGGTTCAT GCCACGACCACGAGTGCGCCAACTGAAGTTACTTGGCAATAA

[1120] ( da2 daldarltt8 fael ) -1 quintuple mutant protein sequences :

[1121] DA2 ( SEQ ID NO : 136 ) :

[1122] MGNKLGRKRQVVEERYTKPQGLYVNKDVDVKKLRKLIVESKLAPCSWGR

[1123] EMS Mutants da l - 4 nucleic acid sequence ( 608OT single base substitution resulting in Ser203Phe substitution ) ( 2020-M2 -247 Tarvense 22863 ) ( SEQ ID NO : 37 ) : ATGGGTTGGTTCAACAAGATCTTCAAAGGCTCTAACCAAAGGCTCCGGGTGGGGAATGAGCACAAT CACAATGGCTATTACGAGAACTATCCAAATGCGCATGCTTCACACGACGAGCCTAGTGCGGATACA GATGCTGATCGTGATGAACCTCAGACGCAGGAATCATCTACATCTGAGGTTAGTAGTGTGTCGCAG AGACTTACTGTTTGACTTTGTTTATAAAACTTTTTTTTGCTGAGTTTGGAGACCTCGAAATGGTCA AATAAGCACAATCTCGGGTTTATCTGATTGTGTATAGCTCTGCTCTAAACTTTTTGTTTACTAGGA TACGTCGAATGACCAGGAAAATGAAGACATAGACCGTGCAATCGCGTTGTCTCTTTTAGAAGAGAA TCAAGGACAGAAAAATACAGGCGGTGAGTCCGCTTTGTCATCATGAAGAGATAGATTTGTTATCAA ATTTTTAAAAAATTTAAAAAGTGAAATTTGGATAACAGGGAAATACGTAATGGTGGATGAAGATGA GCAGCTTGCTAGAGCCATACAAGAAAGTATGATAGTTGGGAATTCACCGCGTCACAAAAGTGGAAG TACGTATGATCATGAGAATGCATATGGGACTGCAGATTTATTTGGGAATGGTCACATGCATGGAGG AGGAAATGTATATGCAAATGGAGATATTTATTACCCAAGACCCGTTGCTTTTTCAATGGATTTCAG GTACTTAGATAGCCAATTATATTTATCGATTTGTTAATTTTCTCCCCAGCCAGATAAACTTGTCAA ACAATTTGTCTGCATAGGATTTGTGCTGGCTGCAATATGGAGATTGGCCATGGAAGATTTCTGAAT TGCCTTAACTCACTATGGCATCCAGAATGTTTTCGATGTTATGGCTGTAGTCACCCGATTTTTGAG TACGAGGTGAAACCAAGCTCTTCCGAACTTTTTAGTTTTCCGGTTCCACTTAACCTTTAAATTTAT CTATAACATGTTTTTTTTTCTTCTTATAGTTTTCAACATCTGGGAACTACCCTTTTCACAAGGCTT GTTACAGGGAGAGATATCATCCAAAATGTGATGTCTGCAACCACTTTGTATGTAAATCTCTTGCCT TTGTATATATTAATTTGCGTTGTTTTCTTTGGATCTTGCACTTATCGGCTGTTGCTTTCTTTTTTC CCTTTGTAAATTTTTTTTGGACAGATTCCAACAAATCCTGCTGGTCTTATTGAATATAGGGCACAC CCATTTTGGATCCAGAAGTATTGCCCTTCTCACGAACACGATTCCACCCCAAGATGTTGCAGTTGT GAAAGAATGGAGGTGACTTCTTTTCTCCTCTAAAATGTCTCAAAACATACAGTACATCTATTCAAA GTCAAAACATTTTTGTGGGAGGAAGTTGACAACTTTTTCTTTATTTTGCAGCCACGGAATACGGGA TATATTGAGCTTAATGATGGACGTAAACTTTGCCTCGAGTGTCTGGACTCAGCGGTCATGGACACA AGTCAATGTCAACCTTTGTACTTGCAGATACAAGATTTCTATGAAGGGCTTAACATGAAGGTAGAG CAGGAAGTTCCACTGCTCTTGGTTGAGAGGCAAGCACTTAACGAAGCCAGAGAAGGTGAAAAGAAT GTGAGTAAAAAAATACAATCATTACTTAGTAATTACTTGAATATACATCACATTTTGCTTCTCCTG AGTTTTTCATTGTGTGCCTCTTAGGGTCACTATCACATGCCAGAGACAAGAGGACTGTGTCTTTCA GAAGAACAAACT GT T AGT ACT AT AAGAAAGCGAT CAAAGGGAAAT TGGGGT GGGAAT AT GATC AC A GAGCCGTACAAGTTAACTCGTCAATGCGAAGTTACTGCCATTCTCATCTTATTTGGGCTCCCTAGG CTACTGACTGGTTCGATTCTAGCTCACGAGATGATGCACGCGTGGATGCGGCTGAAAGGTGAGTTC TTTTGTTCTGCCTTCATTTCTTATTTGGCTGCTTCTTCTCTTGTTTCACATTGCTGAATCGCTATA TTACATTGTGGTTGGTCTTGAAAAGGATTCCGGACACTGAGCCAAGACATTGAAGAAGGTATATGT CAAGTGATGGCTCATAAGTGGATAGAAGCCGAGTTAGCTGCTGGTTCAAGAAACAGCAGTGCTGCA TCATCATCATCATCGTCATCATCTTCTTCTTCTACTAGAGGACTGAAGAAGGGACCAAAATCGCAG TACGAGAGGAAGCTTGGCGAGTTTTTCAAGCACCAGATCGAGTCTGATGCGTCTCCGGTTTATGGA GACGGGTTCAGGGCAGGGCGGCTAGCGGTTAACAAGTATGGTTTGCGAAAAACGCTTGAGCATATA CAGATGACCGGTAGATTCCCGGTTTAA

[1124] DAI-4 protein sequence ( 608C>T single base substitution resulting in a Ser203Phe substitution) ( 2020-M2 -247 Tarvense 22863 ) ( SEQ ID

[1125] NO : 38 ) :

[1126] MGWFNKIFKGSNQRLRVGNEHNHNGYYENYPNAHASHDEPSADTDADRDEPQTQESSTSEDTSNDQ ENEDIDRAIALSLLEENQGQKNTGGKYVMVDEDEQLARAIQESMIVGNSPRHKSGSTYDHGNAYGT ADLFGNGHMHGGGNVYANGDIYYPRPVAFSMDFRICAGCNMEIGHGRFLNCLNSLWHPECFRCYGC SHPIFEYEFSTSGNYPFHKACYRERYHPKCDVCNHFIPTNPAGLIEYRAHPFWIQKYCPSHEHDST PRCCSCERMEPRNTGYIELNDGRKLCLECLDSAVMDTSQCQPLYLQIQDFYEGLNMKVEQEVPLLL VERQALNEAREGEKNGHYHMPETRGLCLSEEQTVSTIRKRSKGNWGGNMITEPYKLTRQCEVTAIL ILFGLPRLLTGSILAHEMMHAWMRLKGFRTLSQDIEEGICQVMAHKWIEAELAAGSRNSSAASSSS SSSSSSSTRGLKKGPKSQYERKLGEFFKHQIESDASPVYGDGFRAGRLAVNKYGLRKTLEHIQMTG RFPV da l-5 nucleic acid sequence ( 764A>G single base substitution resulting in a Tyr255Cys substitution) ( 2020-M2 -655 Tarvense 22863 ) ( SEQ ID NO : 39 ) :

[1127] ATGGGTTGGTTCAACAAGATCTTCAAAGGCTCTAACCAAAGGCTCCGGGTGGGGAATGAGCACAAT

[1128] CACAATGGCTATTACGAGAACTATCCAAATGCGCATGCTTCACACGACGAGCCTAGTGCGGATACA

[1129] GATGCTGATCGTGATGAACCTCAGACGCAGGAATCATCTACATCTGAGGTTAGTAGTGTGTCGCAG

[1130] AGACTTACTGTTTGACTTTGTTTATAAAACTTTTTTTTGCTGAGTTTGGAGACCTCGAAATGGTCA

[1131] AATAAGCACAATCTCGGGTTTATCTGATTGTGTATAGCTCTGCTCTAAACTTTTTGTTTACTAGGA

[1132] TACGTCGAATGACCAGGAAAATGAAGACATAGACCGTGCAATCGCGTTGTCTCTTTTAGAAGAGAA

[1133] TCAAGGACAGAAAAATACAGGCGGTGAGTCCGCTTTGTCATCATGAAGAGATAGATTTGTTATCAA

[1134] ATTTTTAAAAAATTTAAAAAGTGAAATTTGGATAACAGGGAAATACGTAATGGTGGATGAAGATGA

[1135] GCAGCTTGCTAGAGCCATACAAGAAAGTATGATAGTTGGGAATTCACCGCGTCACAAAAGTGGAAG

[1136] TACGTATGATCATGGGAATGCATATGGGACTGCAGATTTATTTGGGAATGGTCACATGCATGGAGG

[1137] AGGAAATGTATATGCAAATGGAGATATTTATTACCCAAGACCCGTTGCTTTTTCAATGGATTTCAG

[1138] GTACTTAGATAGCCAATTATATTTATCGATTTGTTAATCTTTCTCCCCAGCCAGATAAACTTGTCA

[1139] AACAATTTGTCTGCATAGGATTTGTGCTGGCTGCAATATGGAGATTGGCCATGGAAGATTTCTGAA

[1140] TTGCCTTAACTCACTATGGCATCCAGAATGTTTTCGATGTTATGGCTGTAGTCACCCGATTTCTGA

[1141] GTACGAGGTGAAACCAAGCTCTTCCGAACTTTTTAGTTTTCCGGTTCCACTTAACCTTTAAATTTA TCTATAACATGTTTTTTTTTCTTCTTATAGTTTTCAACATCTGGGAACTACCCTTTTCACAAGGCT TGTTACAGGGAGAGATATCATCCAAAATGTGATGTCTGCAACCACTTTGTATGTAAATCTCTTGCC TTTGTATATATTAATTTGCGTTGTTTTCTTTGGATCTTGCACTTATCGGCTGTTGCTTTCTTTTTT CCCTTTGTAAATTTTTTTTGGACAGATTCCAACAAATCCTGCTGGTCTTATTGAATATAGGGCACA CCCATTTTGGATCCAGAAGTGTTGCCCTTCTCACGAACACGATTCCACCCCAAGATGTTGCAGTTG TGAAAGAATGGAGGTGACTTCTTTTCTCCTCTAAAATGTCTCAAAACATACAGTACATCTATTCAA AGTCAAAACATTTTTGTGGGAGGAAGTTGACAACTTTTTCTTTATTTTGCAGCCACGGAATACGGG ATATATTGAGCTTAATGATGGACGTAAACTTTGCCTCGAGTGTCTGGACTCAGCGGTCATGGACAC AAGTCAATGTCAACCTTTGTACTTGCAGATACAAGATTTCTATGAAGGGCTTAACATGAAGGTAGA GCAGGAAGTTCCACTGCTCTTGGTTGAGAGGCAAGCACTTAACGAAGCCAGAGAAGGTGAAAAGAA TGTGAGTAAAAAAATACAATCATTACTTAGTAATTACTTGAATATACATCACATTTTGCTTCTCCT GAGTTTTTCATTGTGTGCCTCTTAGGGTCACTATCACATGCCAGAGACAAGAGGACTGTGTCTTTC AGAAGAACAAACTGTTAGTACTATAAGAAAGCGATCAAAGGGAAATTGGGGTGGGAATATGATCAC AGAGCCGTACAAGTTAACTCGTCAATGCGAAGTTACTGCCATTCTCATCTTATTTGGGCTCCCTAG GCTACTGACTGGTTCGATTCTAGCTCACGAGATGATGCACGCGTGGATGCGGCTGAAAGGTGAGTT CTTTTGTTCTGCCTTCATTTCTTATTTGGCTGCTTCTTCTCTTGTTTCACATTGCTGAATCGCTAT ATTACATTGTGGTTGGTCTTGAAAAGGATTCCGGACACTGAGCCAAGACATTGAAGAAGGTATATG TCAAGTGATGGCTCATAAGTGGATAGAAGCCGAGTTAGCTGCTGGTTCAAGAAACAGCAGTGCTGC ATCATCATCATCATCGTCATCATCTTCTTCTTCTACTAGAGGACTGAAGAAGGGACCAAAATCGCA GTACGAGAGGAAGCTTGGCGAGTTTTTCAAGCACCAGATCGAGTCTGATGCGTCTCCGGTTTATGG AGACGGGTTCAGGGCAGGGCGGCTAGCGGTTAACAAGTATGGTTTGCGAAAAACGCTTGAGCATAT ACAGATGACCGGTAGATTCCCGGTTTAA

[1142] DAI-5 protein sequence ( 764A>G single base substitution resulting in Tyr255Cys substitution ) ( 2020-M2-655 Tarvense 22863 ) ( SEQ ID NO : 40 ) :

[1143] MGWFNKIFKGSNQRLRVGNEHNHNGYYENYPNAHASHDEPSADTDADRDEPQTQESSTSEDTSNDQ ENEDIDRAIALSLLEENQGQKNTGGKYVMVDEDEQLARAIQESMIVGNSPRHKSGSTYDHGNAYGT ADLFGNGHMHGGGNVYANGDIYYPRPVAFSMDFRICAGCNMEIGHGRFLNCLNSLWHPECFRCYGC

[1144] SHPISEYEFSTSGNYPFHKACYRERYHPKCDVCNHFIPTNPAGLIEYRAHPFWIQKCCPSHEHDST PRCCSCERMEPRNTGYIELNDGRKLCLECLDSAVMDTSQCQPLYLQIQDFYEGLNMKVEQEVPLLL VERQALNEAREGEKNGHYHMPETRGLCLSEEQTVSTIRKRSKGNWGGNMITEPYKLTRQCEVTAIL

[1145] ILFGLPRLLTGSILAHEMMHAWMRLKGFRTLSQDIEEGICQVMAHKWIEAELAAGSRNSSAASSSS SSSSSSSTRGLKKGPKSQYERKLGEFFKHQIESDASPVYGDGFRAGRLAVNKYGLRKTLEHIQMTG

[1146] RFPV da l-6 nucleic acid sequence ( 1032delG single base deletion resulting in a frame shift ) ( 2020-M2 -203 Tarvense 22863 ) ( SEQ ID NO : 41 ) :

[1147] ATGGGTTGGTTCAACAAGATCTTCAAAGGCTCTAACCAAAGGCTCCGGGTGGGGAATGAGCACAAT

[1148] CACAATGGCTATTACGAGAACTATCCAAATGCGCATGCTTCACACGACGAGCCTAGTGCGGATACA

[1149] GATGCTGATCGTGATGAACCTCAGACGCAGGAATCATCTACATCTGAGGTTAGTAGTGTGTCGCAG

[1150] AGACTTACTGTTTGACTTTGTTTATAAAACTTTTTTTTGCTGAGTTTGGAGACCTCGAAATGGTCA

[1151] AATAAGCACAATCTCGGGTTTATCTGATTGTGTATAGCTCTGCTCTAAACTTTTTGTTTACTAGGA

[1152] TACGTCGAATGACCAGGAAAATGAAGACATAGACCGTGCAATCGCGTTGTCTCTTTTAGAAGAGAA

[1153] TCAAGGACAGAAAAATACAGGCGGTGAGTCCGCTTTGTCATCATGAAGAGATAGATTTGTTATCAA

[1154] ATTTTTAAAAAATTTAAAAAGTGAAATTTGGATAACAGGGAAATACGTAATGGTGGATGAAGATGA

[1155] GCAGCTTGCTAGAGCCATACAAGAAAGTATGATAGTTGGGAATTCACCGCGTCACAAAAGTGGAAG

[1156] TACGTATGATCATGGGAATGCATATGGGACTGCAGATTTATTTGGGAATGGTCACATGCATGGAGG

[1157] AGGAAATGTATATGCAAATGGAGATATTTATTACCCAAGACCCGTTGCTTTTTCAATGGATTTCAG

[1158] GTACTTAGATAGCCAATTATATTTATCGATTTGTTAATTTTCTCCCCAGCCAGATAAACTTGTCAA

[1159] ACAATTTGTCTGCATAGGATTTGTGCTGGCTGCAATATGGAGATTGGCCATGGAAGATTTCTGAAT

[1160] TGCCTTAACTCACTATGGCATCCAGAATGTTTTCGATGTTATGGCTGTAGTCACCCGATTTCTGAG

[1161] TACGAGGTGAAACCAAGCTCTTCCGAACTTTTTAGTTTTCCGGTTCCACTTAACCTTTAAATTTAT

[1162] CTATAACATGTTTTTTTTTCTTCTTATAGTTTTCAACATCTGGGAACTACCCTTTTCACAAGGCTT

[1163] GTTACAGGGAGAGATATCATCCAAAATGTGATGTCTGCAACCACTTTGTATGTAAATCTCTTGCCT

[1164] TTGTATATATTAATTTGCGTTGTTTTCTTTGGATCTTGCACTTATCGGCTGTTGCTTTCTTTTTTC

[1165] CCTTTGTAAATTTTTTTTGGACAGATTCCAACAAATCCTGCTGGTCTTATTGAATATAGGGCACAC

[1166] CCATTTTGGATCCAGAAGTATTGCCCTTCTCACGAACACGATTCCACCCCAAGATGTTGCAGTTGT

[1167] GAAAGAATGGAGGTGACTTCTTTTCTCCTCTAAAATGTCTCAAAACATACAGTACATCTATTCAAA

[1168] GTCAAAACATTTTTGTGGGAGGAAGTTGACAACTTTTTCTTTATTTTGCAGCCACGGAATACGGGA

[1169] TATATTGAGCTTAATGATGGACGTAAACTTTGCCTCGAGTGTCTGGACTCAGCGGTCATGGACACA

[1170] AGTCAATGTCAACCTTTGTACTTGCAGATACAAGATTTCTATGAAGGGCTTAACATGAAGGTAGAG

[1171] CAGGAAGTTCCACTGCTCTTGGTTGAGAGGCAAGCACTTAACGAAGCCAGAGAAGGTGAAAAAATG

[1172] TGAGTAAAAAAATACAATCATTACTTAGTAATTACTTGAATATACATCACATTTTGCTTCTCCTGA

[1173] GTTTTTCATTGTGTGCCTCTTAGGGTCACTATCACATGCCAGAGACAAGAGGACTGTGTCTTTCAG

[1174] AAGAACAAACTGTTAGTACTATAAGAAAGCGATCAAAGGGAAATTGGGGTGGGAATATGATCACAG

[1175] AGCCGTACAAGTTAACTCGTCAATGCGAAGTTACTGCCATTCTCATCTTATTTGGGCTCCCTAGGC

[1176] TACTGACTGGTTCGATTCTAGCTCACGAGATGATGCACGCGTGGATGCGGCTGAAAGGTGAGTTCT

[1177] TTTGTTCTGCCTTCATTTCTTATTTGGCTGCTTCTTCTCTTGTTTCACATTGCTGAATCGCTATAT

[1178] TACATTGTGGTTGGTCTTGAAAAGGATTCCGGACACTGAGCCAAGACATTGAAGAAGGTATATGTC

[1179] AAGTGATGGCTCATAAGTGGATAGAAGCCGAGTTAGCTGCTGGTTCAAGAAACAGCAGTGCTGCAT

[1180] CATCATCATCATCGTCATCATCTTCTTCTTCTACTAGAGGACTGAAGAAGGGACCAAAATCGCAGT

[1181] ACGAGAGGAAGCTTGGCGAGTTTTTCAAGCACCAGATCGAGTCTGATGCGTCTCCGGTTTATGGAG

[1182] ACGGGTTCAGGGCAGGGCGGCTAGCGGTTAACAAGTATGGTTTGCGAAAAACGCTTGAGCATATAC AGATGACCGGTAGATTCCCGGTTTAA

[1183] DAI-6 protein sequence ( 1032delG single base deletion resulting in a frame shift ) ( 2020-M2-203 Tarvense 22863 ) ( SEQ ID NO : 42 ) :

[1184] MGWFNKIFKGSNQRLRVGNEHNHNGYYENYPNAHASHDEPSADTDADRDEPQTQESSTSEDTSNDQ

[1185] ENEDIDRAIALSLLEENQGQKNTGGKYVMVDEDEQLARAIQESMIVGNSPRHKSGSTYDHGNAYGT

[1186] ADLFGNGHMHGGGNVYANGDIYYPRPVAFSMDFRICAGCNMEIGHGRFLNCLNSLWHPECFRCYGC

[1187] SHPISEYEFSTSGNYPFHKACYRERYHPKCDVCNHFIPTNPAGLIEYRAHPFWIQKYCPSHEHDST

[1188] PRCCSCERMEPRNTGYIELNDGRKLCLECLDSAVMDTSQCQPLYLQIQDFYEGLNMKVEQEVPLLL VERQALNEAREGEKN darl -4 nucleic acid sequence ( 1354OT s ingle base substitution resulting in an Arg452Cys substitution ) ( 2020 -M2- 036 Tarvense 15572 ) ( SEQ ID NO : 47 ) :

[1189] ATGGAGTTTCTTCTCTTCTTTGGATACCTTAAAGCAGTGTTTCTCTTTGCAGGTAAAGGATTGTTG GTGCCAATGGGGTGGCTTACTAAAATCCTTAAAGGCTCAAGCCATAAATTCTCGGATGGACAAGTT AACAGAAGATATAGAGATGATAGGAACTTGGAAAGTTCTCGTCTTTCTGCGGTAAGTGTCAGTTTC GTCTTCTGTGTTCTCTGGTTCATCCTTTCTTATTTGAAGAAAGATGTCATTTTGAAGTGAAGGTTA AAGAAAAT GGT AT C T T GG AACT T T GAT T C AGGAT GGAT C TG AT TT T G AC AAAG AAGAGAT T GAAT G T GCT AT TGCACT CT CT CT TTCAGAAC AAGAACAT GT AAT TCCACAAGAT GACAAAGGAAAACAAGT AATCGGTAAGGTTGTCTATTCGCTGGTTGTTTGCATTTGTATAGAGAGAAGAAACAACACACAAGA AAAAGAGAAGAAAGGAAGTTGTGTGAAACTTTATCTTGCTAAGCTGTTTTCTTTTACCTCGTATTC T T T AGAAT AC AAAT CT GAAACC GAAG AAGAT GAGGAT G AGGAT GAGG AC GAAG AT GAAG AAT AC AC GAGAGCTCAGCTAGAAGCAGCGGAAGAAGAGGAAAGACGGGCAGCTCAAGCTCAAATCGAGGAAGA AGAGAAACGGCGAGCTGAAGCTGAATTAGAAGAAATAGAGAAACAGCTTGCGAAAGCTCGACTAGA AGAGGAAGAAGTTAGGCGTACCAAAGCGCAATTGGAGGAAGACGAGCAACTAGCTAAGGCCATTCA AGAAAGTATGGTTGTGGGATCTCCTCCTCGGTATGATTCTGGAAGTATGTTTCAACCATTCCCTTT CCTTAATCCATCTAGTCACAGGTAAATGTTTCAAAAGTTAATTCAATATCCACAACTTAGAATTCA GATACACTGTCATTCTCTTACAATTTTGCTGCAATGAGTATTTTTTCTTTTTCTTTGTTTCTTTAT TGCTTTTGAGGGTATGTACTGGTTGCCTAGCTGAGATTGGACATGGAAGGTTTCTGAGTTGCATGG GTGGCGTTTGGCATCCTGAATGTTTCTGCTGCCATGCTTGTGATAAGCCCATCATAGATTATGAGG TCACAAGACATTTCGTTGAGTAGTGTTACATTGTCCCGAATCTAGTGAATCCTAGCTTGTTAAGTT TTGTTTTTACAGTTCTCAATGTCCGGGAATCGGCCTTATCACAAGCTATGTTACAAGGAGCAGCAT CATCCTAAATGTGATGTTTGCCATAACTTTGTGAGTGATTCCGACCTTTTTATTGATATCATCTTC TGCAAACGATGTTATCAAGTGGTACCAAAGCTTAAATGTGTATGCGTGAAATTAACTCCTATAGAT TCCGACGAATTCAGCTGGTCTCATTGAGTACAGGGCACATCCCTTTTGGATGCAAAAGTATTGTCC TTCACATGAGCGTGATGGAACTCCTCGTTGCTGTAGCTGTGAGCGAATGGAGGTCAGTTTCGATAT GATATATCTACTAATAGGGAAACAATTCTAAGAGACGTTTTTGAGATTTGATGTTGTTCTGTGTGT TTGCTCACCGGTTACAGCCAAAAGATACAAAATATCTCATTCTTGATGATGGTCGAAAACTGTGTC TTGAATGTCTCGACTCAGCCATTATGGACACTCACGAATGCCAACCATTGTACCTTGAGATACGTG AGTTTTATGAAGGCTTACACATGAAAGTAGAACAGCAGATTCCTATGCTCTTGGTGGAGAGATCAG CTTTAAACGAAGCTATGGAAGGAGAGAAACACGTAAAAACCGCTCTTTATTTATCCTATGTTGCTT ACATAAACACTCTTTTTGCTCTTGCTCATTTATTTTTAACTTACTTTCTATTTATTTTTGTTGCAG GGACATCATCATTTACCTGAAACCAGAGGACTCTGTTTGTCTGAGGAACAAACTGTCACAACGGTA AATTGCTCTCACTTTCGTAAGCAGCTATTTCTATCCGCCACAAATTGTGTTTGAATCAGAAAGAGT TTCTTGTAGGTGTTAAGGAGACCAAGAATTGGGGCAGGCTACAAATTGATAGACATGATCACAGAG CCTTGCAGGCTGATACGTCGTTGTGAAGTCACTGCAATTCTCATTCTATATGGACTTCCTTGGTAA AAAAAAAGAATTACTTCCCAACACATTCTTAAACCGACTGTTCTTTCTTGATGAGATAGTTAAAAT ATGTGTATTACAGTTTGTTAACTGGATCAATCCTTGCTCATGAGATGATGCATGCCTGGCTTCGAC TAAATGGTAATTTGAAGAACCCTTCCTTCTTTTCTCGGTACATTAGTAATAATTGCTCTTTGATGT TTTGTTGCTCTCTAAATGTATGAGTAGGGTATCCGAATCTTAGGCCAGAAGTTGAAGAAGGAATAT GTCAGGTTTTGGCTCACATGTGGTTGGAATCTGAGACTTATGCTGGCTCTACATTAGTAGATATCG CATCTTCTTCATCATCATCATCATCGGCTGTGGTCACTGCATCGTCAAAGAAAGGTGAGAGGTCTG ACTTTGAGAAGAAACTTGGTGAGTTTTTCAAGCACCAAATAGAGTCGGATTCTTCGTCGGCATATG GGGATGGGTTCAGGCAAGGTAACCAAGCAGTTCTTACGCACGGTCTGAGGCGAACCCTCGACCATA TTCGCTTGACCGGTACATTTCCTTAA DARI -4 protein sequence ( 1354OT s ingle base substitution resulting in an Arg452Cys substitution ) ( 2020-M2 - 036 Tarvense 15572 ) ( SEQ ID NO : 48 ) :

[1190] ME FLLFFGYLKAVFLFAGKGLLVPMGWLTKILKGSSHKFSDGQVNRRYRDDRNLESSRLSADGSDF DKEEIECAIALSLSEQEHVIPQDDKGKQVIEYKSETEEDEDEDEDEDEEYTRAQLEAAEEEERRAA QAQIEEEEKRRAEAELEE IEKQLAKARLEEEEVRRTKAQLEEDEQLAKAIQESMVVGSPPRYDSGS MFQPFPFLNPSSHRVCTGCLAE IGHGRFLSCMGGVWHPECFCCHACDKPI IDYEFSMSGNRPYHKL CYKEQHHPKCDVCHNFIPTNSAGLIEYRAHPFWMQKYCPSHERDGTPRCCSCERMEPKDTKYLILD DGRKLCLECLDSAIMDTHECQPLYLE IREFYEGLHMKVEQQIPMLLVERSALNEAMEGEKHGHHHL PETRGLCLSEEQTVTTVLRRPRIGAGYKLIDMITEPCRLIRRCEVTAILILYGLPCLLTGSILAHE MMHAWLRLNGYPNLRPEVEEGICQVLAHMWLESETYAGSTLVDIASSSSSSSSAVVTASSKKGERS DFEKKLGEFFKHQIESDSSSAYGDGFRQGNQAVLTHGLRRTLDHIRLTGTFP darl -5 ( 1108OT single base substitution resulting in premature stop codon ) ( Sec 7 3 Tarvense 15572 ) ( SEQ ID NO : 49 ) :

[1191] ATGGAGTTTCTTCTCTTCTTTGGATACCTTAAAGCAGTGTTTCTCTTTGCAGGTAAAGGATTGTTG GTGCCAATGGGGTGGCTTACTAAAATCCTTAAAGGCTCAAGCCATAAATTCTCGGATGGACAAGTT AACAGAAGATATAGAGATGATAGGAACTTGGAAAGTTCTCGTCTTTCTGCGGTAAGTGTCAGTTTC GTCTTCTGTGTTCTCTGGTTCATCCTTTCTTATTTGAAGAAAGATGTCATTTTGAAGTGAAGGTTA AAGAAAAT GGT AT C T T GG AACT T T GAT T C AGGAT GGAT C TG AT TT T G AC AAAGAAGAGAT T GAAT G T GCT AT TGCACT CT CT CT TTCAGAAC AAGAACAT GTAAT TCCACAAGAT GACAAAGGAAAACAAGT AATCGGTAAGGTTGTCTATTCGCTGGTTGTTTGCATTTGTATAGAGAGAAGAAACAACACACAAGA AAAAGAGAAGAAAGGAAGTTGTGTGAAACTTTATCTTGCTAAGCTGTTTTCTTTTACCTCGTATTC T T T AGAAT AC AAAT CT GAAACC GAAG AAGAT GAGGAT G AGGAT GAGG AC GAAGAT GAAG AAT AC AC GAGAGCTCAGCTAGAAGCAGCGGAAGAAGAGGAAAGACGGGCAGCTCAAGCTCAAATCGAGGAAGA AGAGAAACGGCGAGCTGAAGCTGAATTAGAAGAAATAGAGAAACAGCTTGCGAAAGCTCGACTAGA AGAGGAAGAAGTTAGGCGTACCAAAGCGCAATTGGAGGAAGACGAGCAACTAGCTAAGGCCATTCA AGAAAGTATGGTTGTGGGATCTCCTCCTCGGTATGATTCTGGAAGTATGTTTCAACCATTCCCTTT CCTTAATCCATCTAGTCACAGGTAAATGTTTCAAAAGTTAATTCAATATCCACAACTTAGAATTCA GATACACTGTCATTCTCTTACAATTTTGCTGCAATGAGTATTTTTTCTTTTTCTTTGTTTCTTTAT TGCTTTTGAGGGTATGTACTGGTTGCCTAGCTGAGATTGGACATGGAAGGTTTCTGAGTTGCATGG GTGGCGTTTGGCATCCTGAATGTTTCTGCTGCCATGCTTGTGATAAGCCCATCATAGATTATGAGG TCACAAGACATTTCGTTGAGTAGTGTTACATTGTCCCGAATCTAGTGAATCCTAGCTTGTTAAGTT TTGTTTTTACAGTTCTCAATGTCCGGGAATCGGCCTTATCACAAGCTATGTTACAAGGAGCAGCAT CATCCTAAATGTGATGTTTGCCATAACTTTGTGAGTGATTCCGACCTTTTTATTGATATCATCTTC TGCAAACGATGTTATCAAGTGGTACCAAAGCTTAAATGTGTATGCGTGAAATTAACTCCTATAGAT TCCGACGAATTCAGCTGGTCTCATTGAGTACAGGGCACATCCCTTTTGGATGCAAAAGTATTGTCC TTCACATGAGCGTGATGGAACTCCTCGTTGCTGTAGCTGTGAGCGAATGGAGGTCAGTTTCGATAT GATATATCTACTAATAGGGAAACAATTCTAAGAGACGTTTTTGAGATTTGATGTTGTTCTGTGTGT TTGCTCACCGGTTACAGCCAAAAGATACAAAATATCTCATTCTTGATGATGGTCGAAAACTGTGTC TTGAATGTCTCGACTCAGCCATTATGGACACTCACGAATGCCAACCATTGTACCTTGAGATACGTG AGTTTTATGAAGGCTTACACATGAAAGTAGAATAGCAGATTCCTATGCTCTTGGTGGAGAGATCAG CTTTAAACGAAGCTATGGAAGGAGAGAAACACGTAAAAACCGCTCTTTATTTATCCTATGTTGCTT ACATAAACACTCTTTTTGCTCTTGCTCATTTATTTTTAACTTACTTTCTATTTATTTTTGTTGCAG GGACATCATCATTTACCTGAAACCAGAGGACTCTGTTTGTCTGAGGAACAAACTGTCACAACGGTA AATTGCTCTCACTTTCGTAAGCAGCTATTTCTATCCGCCACAAATTGTGTTTGAATCAGAAAGAGT TTCTTGTAGGTGTTAAGGAGACCAAGAATTGGGGCAGGCTACAAATTGATAGACATGATCACAGAG CCTTGCAGGCTGATACGTCGTTGTGAAGTCACTGCAATTCTCATTCTATATGGACTTCCTCGGTAA AAAAAAAGAATTACTTCCCAACACATTCTTAAACCGACTGTTCTTTCTTGATGAGATAGTTAAAAT ATGTGTATTACAGTTTGTTAACTGGATCAATCCTTGCTCATGAGATGATGCATGCCTGGCTTCGAC TAAATGGTAATTTGAAGAACCCTTCCTTCTTTTCTCGGTACATTAGTAATAATTGCTCTTTGATGT TTTGTTGCTCTCTAAATGTATGAGTAGGGTATCCGAATCTTAGGCCAGAAGTTGAAGAAGGAATAT GTCAGGTTTTGGCTCACATGTGGTTGGAATCTGAGACTTATGCTGGCTCTACATTAGTAGATATCG CATCTTCTTCATCATCATCATCATCGGCTGTGGTCACTGCATCGTCAAAGAAAGGTGAGAGGTCTG ACTTTGAGAAGAAACTTGGTGAGTTTTTCAAGCACCAAATAGAGTCGGATTCTTCGTCGGCATATG GGGATGGGTTCAGGCAAGGTAACCAAGCAGTTCTTACGCACGGTCTGAGGCGAACCCTCGACCATA TTCGCTTGACCGGTACATTTCCTTAA

[1192] DARI -5 protein sequence ( 1108C>T s ingle base substitution resulting in premature stop codon ) ( Sec 7 3 Tarvense 15572 ) ( SEQ ID NO : 50 ) :

[1193] ME FLLFFGYLKAVFLFAGKGLLVPMGWLTKILKGSSHKFSDGQVNRRYRDDRNLESSRLSADGSDF DKEEIECAIALSLSEQEHVIPQDDKGKQVIEYKSETEEDEDEDEDEDEEYTRAQLEAAEEEERRAA QAQIEEEEKRRAEAELEE IEKQLAKARLEEEEVRRTKAQLEEDEQLAKAIQESMVVGSPPRYDSGS MFQPFPFLNPSSHRVCTGCLAE IGHGRFLSCMGGVWHPECFCCHACDKPI IDYEFSMSGNRPYHKL CYKEQHHPKCDVCHNFIPTNSAGLIEYRAHPFWMQKYCPSHERDGTPRCCSCERMEPKDTKYLILD DGRKLCLECLDSAIMDTHECQPLYLE IREFYEGLHMKVEQ darl - 6 nucleic acid sequence ( 1486G>A s ingle base substitution resulting in Glu496Lys substitution ) ( 2020 M2 044 Tarvense 15572 ) ( SEQ ID NO : 51 ) :

[1194] ATGGAGTTTCTTCTCTTCTTTGGATACCTTAAAGCAGTGTTTCTCTTTGCAGGTAAAGGATTGTTG GTGCCAATGGGGTGGCTTACTAAAATCCTTAAAGGCTCAAGCCATAAATTCTCGGATGGACAAGTT AACAGAAGATATAGAGATGATAGGAACTTGGAAAGTTCTCGTCTTTCTGCGGTAAGTGTCAGTTTC GTCTTCTGTGTTCTCTGGTTCATCCTTTCTTATTTGAAGAAAGATGTCATTTTGAAGTGAAGGTTA AAGAAAAT GGT AT C T T GG AACT T T GAT T C AGGAT GGAT C TG AT TT T G AC AAAG AAGAGAT T GAAT G T GCT AT TGCACT CT CT CT TTCAGAACAAGAACAT GTAAT TCCACAAGAT GACAAAGGAAAACAAGT AATCGGTAAGGTTGTCTATTCGCTGGTTGTTTGCATTTGTATAGAGAGAAGAAACAACACACAAGA AAAAGAGAAGAAAGGAAGTTGTGTGAAACTTTATCTTGCTAAGCTGTTTTCTTTTACCTCGTATTC T T T AGAAT AC AAAT CT GAAACC GAAG AAGAT GAGGAT G AGGAT GAGG AC GAAG AT GAAG AAT AC AC GAGAGCTCAGCTAGAAGCAGCGGAAGAAGAGGAAAGACGGGCAGCTCAAGCTCAAATCGAGGAAGA AGAGAAACGGCGAGCTGAAGCTGAATTAGAAGAAATAGAGAAACAGCTTGCGAAAGCTCGACTAGA AGAGGAAGAAGTTAGGCGTACCAAAGCGCAATTGGAGGAAGACGAGCAACTAGCTAAGGCCATTCA AGAAAGTATGGTTGTGGGATCTCCTCCTCGGTATGATTCTGGAAGTATGTTTCAACCATTCCCTTT CCTTAATCCATCTAGTCACAGGTAAATGTTTCAAAAGTTAATTCAATATCCACAACTTAGAATTCA GATACACTGTCATTCTCTTACAATTTTGCTGCAATGAGTATTTTTTCTTTTTCTTTGTTTCTTTAT TGCTTTTGAGGGTATGTACTGGTTGCCTAGCTGAGATTGGACATGGAAGGTTTCTGAGTTGCATGG GTGGCGTTTGGCATCCTGAATGTTTCTGCTGCCATGCTTGTGATAAGCCCATCATAGATTATGAGG TCACAAGACATTTCGTTGAGTAGTGTTACATTGTCCCGAATCTAGTGAATCCTAGCTTGTTAAGTT TTGTTTTTACAGTTCTCAATGTCCGGGAATCGGCCTTATCACAAGCTATGTTACAAGGAGCAGCAT CATCCTAAATGTGATGTTTGCCATAACTTTGTGAGTGATTCCGACCTTTTTATTGATATCATCTTC TGCAAACGATGTTATCAAGTGGTACCAAAGCTTAAATGTGTATGCGTGAAATTAACTCCTATAGAT TCCGACGAATTCAGCTGGTCTCATTGAGTACAGGGCACATCCCTTTTGGATGCAAAAGTATTGTCC TTCACATGAGCGTGATGGAACTCCTCGTTGCTGTAGCTGTGAGCGAATGGAGGTCAGTTTCGATAT GATATATCTACTAATAGGGAAACAATTCTAAGAGACGTTTTTGAGATTTGATGTTGTTCTGTGTGT TTGCTCACCGGTTACAGCCAAAAGATACAAAATATCTCATTCTTGATGATGGTCGAAAACTGTGTC TTGAATGTCTCGACTCAGCCATTATGGACACTCACGAATGCCAACCATTGTACCTTGAGATACGTG AGTTTTATGAAGGCTTACACATGAAAGTAGAACAGCAGATTCCTATGCTCTTGGTGGAGAGATCAG CTTTAAACGAAGCTATGGAAGGAGAGAAACACGTAAAAACCGCTCTTTATTTATCCTATGTTGCTT ACATAAACACTCTTTTTGCTCTTGCTCATTTATTTTTAACTTACTTTCTATTTATTTTTGTTGCAG GGACATCATCATTTACCTGAAACCAGAGGACTCTGTTTGTCTGAGGAACAAACTGTCACAACGGTA AATTGCTCTCACTTTCGTAAGCAGCTATTTCTATCCGCCACAAATTGTGTTTGAATCAGAAAGAGT TTCTTGTAGGTGTTAAGGAGACCAAGAATTGGGGCAGGCTACAAATTGATAGACATGATCACAGAG CCTTGCAGGCTGATACGTCGTTGTGAAGTCACTGCAATTCTCATTCTATATGGACTTCCTCGGTAA AAAAAAAGAATTACTTCCCAACACATTCTTAAACCGACTGTTCTTTCTTGATGAGATAGTTAAAAT ATGTGTATTACAGTTTGTTAACTGGATCAATCCTTGCTCATGAGATGATGCATGCCTGGCTTCGAC TAAATGGTAATTTGAAGAACCCTTCCTTCTTTTCTCGGTACATTAGTAATAATTGCTCTTTGATGT TTTGTTGCTCTCTAAATGTATGAGTAGGGTATCCGAATCTTAGGCCAGAAGTTGAAGAAGGAATAT GTCAGGTTTTGGCTCACATGTGGTTGGAATCTAAGACTTATGCTGGCTCTACATTAGTAGATATCG CATCTTCTTCATCATCATCATCATCGGCTGTGGTCACTGCATCGTCAAAGAAAGGTGAGAGGTCTG ACTTTGAGAAGAAACTTGGTGAGTTTTTCAAGCACCAAATAGAGTCGGATTCTTCGTCGGCATATG GGGATGGGTTCAGGCAAGGTAACCAAGCAGTTCTTACGCACGGTCTGAGGCGAACCCTCGACCATA TTCGCTTGACCGGTACATTTCCTTAA

[1195] DARI - 6 protein sequence ( 1486G>A s ingle base substitution resulting in a Glu496Lys substitution ) ( 2020 M2 044 Tarvense 15572 ) ( SEQ ID NO : 52 ) :

[1196] ME FLLFFGYLKAVFLFAGKGLLVPMGWLTKILKGSSHKFSDGQVNRRYRDDRNLESSRLSADGSDF DKEEIECAIALSLSEQEHVIPQDDKGKQVIEYKSETEEDEDEDEDEDEEYTRAQLEAAEEEERRAA QAQIEEEEKRRAEAELEE IEKQLAKARLEEEEVRRTKAQLEEDEQLAKAIQESMVVGSPPRYDSGS MFQPFPFLNPSSHRVCTGCLAE IGHGRFLSCMGGVWHPECFCCHACDKPI IDYEFSMSGNRPYHKL CYKEQHHPKCDVCHNFIPTNSAGLIEYRAHPFWMQKYCPSHERDGTPRCCSCERMEPKDTKYLILD DGRKLCLECLDSAIMDTHECQPLYLE IREFYEGLHMKVEQQIPMLLVERSALNEAMEGEKHGHHHL PETRGLCLSEEQTVTTVLRRPRIGAGYKLIDMITEPCRLIRRCEVTAILILYGLPRLLTGSILAHE MMHAWLRLNGYPNLRPEVEEGICQVLAHMWLESKTYAGSTLVDIASSSSSSSSAVVTASSKKGERS DFEKKLGEFFKHQIESDSSSAYGDGFRQGNQAVLTHGLRRTLDHIRLTGTFP darl - 7 nucleic acid sequence ( 769G>A s ingle base substitution resulting in a Gly257Arg substitution ) ( 2020-M2 -7 60 Tarvense 15572 ) ( SEQ ID NO : 53 ) :

[1197] ATGGAGTTTCTTCTCTTCTTTGGATACCTTAAAGCAGTGTTTCTCTTTGCAGGTAAAGGATTGTTG GTGCCAATGGGGTGGCTTACTAAAATCCTTAAAGGCTCAAGCCATAAATTCTCGGATGGACAAGTT AACAGAAGATATAGAGATGATAGGAACTTGGAAAGTTCTCGTCTTTCTGCGGTAAGTGTCAGTTTC GTCTTCTGTGTTCTCTGGTTCATCCTTTCTTATTTGAAGAAAGATGTCATTTTGAAGTGAAGGTTA AAGAAAAT GGT AT C T T GG AACT T T GAT T C AGGAT GGAT C TG AT TT T G AC AAAG AAGAGAT T GAAT G T GCT AT TGCACT CT CT CT TTCAGAAC AAGAACAT GT AAT TCCACAAGAT GACAAAGGAAAACAAGT AATCGGTAAGGTTGTCTATTCGCTGGTTGTTTGCATTTGTATAGAGAGAAGAAACAACACACAAGA AAAAGAGAAGAAAGGAAGTTGTGTGAAACTTTATCTTGCTAAGCTGTTTTCTTTTACCTCGTATTC T T T AGAAT AC AAAT CT GAAACC GAAG AAGAT GAGGAT G AGGAT GAGG AC GAAG AT GAAG AAT AC AC GAGAGCTCAGCTAGAAGCAGCGGAAGAAGAGGAAAGACGGGCAGCTCAAGCTCAAATCGAGGAAGA AGAGAAACGGCGAGCTGAAGCTGAATTAGAAGAAATAGAGAAACAGCTTGCGAAAGCTCGACTAGA AGAGGAAGAAGTTAGGCGTACCAAAGCGCAATTGGAGGAAGACGAGCAACTAGCTAAGGCCATTCA AGAAAGTATGGTTGTGGGATCTCCTCCTCGGTATGATTCTGGAAGTATGTTTCAACCATTCCCTTT CCTTAATCCATCTAGTCACAGGTAAATGTTTCAAAAGTTAATTCAATATCCACAACTTAGAATTCA GATACACTGTCATTCTCTTACAATTTTGCTGCAATGAGTATTTTTTCTTTTTCTTTGTTTCTTTAT TGCTTTTGAGGGTATGTACTGGTTGCCTAGCTGAGATTGGACATGGAAGGTTTCTGAGTTGCATGG GTGGCGTTTGGCATCCTGAATGTTTCTGCTGCCATGCTTGTGATAAGCCCATCATAGATTATGAGG TCACAAGACATTTCGTTGAGTAGTGTTACATTGTCCCGAATCTAGTGAATCCTAGCTTGTTAAGTT TTGTTTTTACAGTTCTCAATGTCCAGGAATCGGCCTTATCACAAGCTATGTTACAAGGAGCAGCAT CATCCTAAATGTGATGTTTGCCATAACTTTGTGAGTGATTCCGACCTTTTTATTGATATCATCTTC TGCAAACGATGTTATCAAGTGGTACCAAAGCTTAAATGTGTATGCGTGAAATTAACTCCTATAGAT TCCGACGAATTCAGCTGGTCTCATTGAGTACAGGGCACATCCCTTTTGGATGCAAAAGTATTGTCC TTCACATGAGCGTGATGGAACTCCTCGTTGCTGTAGCTGTGAGCGAATGGAGGTCAGTTTCGATAT GATATATCTACTAATAGGGAAACAATTCTAAGAGACGTTTTTGAGATTTGATGTTGTTCTGTGTGT TTGCTCACCGGTTACAGCCAAAAGATACAAAATATCTCATTCTTGATGATGGTCGAAAACTGTGTC TTGAATGTCTCGACTCAGCCATTATGGACACTCACGAATGCCAACCATTGTACCTTGAGATACGTG AGTTTTATGAAGGCTTACACATGAAAGTAGAACAGCAGATTCCTATGCTCTTGGTGGAGAGATCAG CTTTAAACGAAGCTATGGAAGGAGAGAAACACGTAAAAACCGCTCTTTATTTATCCTATGTTGCTT ACATAAACACTCTTTTTGCTCTTGCTCATTTATTTTTAACTTACTTTCTATTTATTTTTGTTGCAG GGACATCATCATTTACCTGAAACCAGAGGACTCTGTTTGTCTGAGGAACAAACTGTCACAACGGTA AATTGCTCTCACTTTCGTAAGCAGCTATTTCTATCCGCCACAAATTGTGTTTGAATCAGAAAGAGT TTCTTGTAGGTGTTAAGGAGACCAAGAATTGGGGCAGGCTACAAATTGATAGACATGATCACAGAG CCTTGCAGGCTGATACGTCGTTGTGAAGTCACTGCAATTCTCATTCTATATGGACTTCCTCGGTAA AAAAAAAGAATTACTTCCCAACACATTCTTAAACCGACTGTTCTTTCTTGATGAGATAGTTAAAAT ATGTGTATTACAGTTTGTTAACTGGATCAATCCTTGCTCATGAGATGATGCATGCCTGGCTTCGAC TAAATGGTAATTTGAAGAACCCTTCCTTCTTTTCTCGGTACATTAGTAATAATTGCTCTTTGATGT TTTGTTGCTCTCTAAATGTATGAGTAGGGTATCCGAATCTTAGGCCAGAAGTTGAAGAAGGAATAT GTCAGGTTTTGGCTCACATGTGGTTGGAATCTGAGACTTATGCTGGCTCTACATTAGTAGATATCG CATCTTCTTCATCATCATCATCATCGGCTGTGGTCACTGCATCGTCAAAGAAAGGTGAGAGGTCTG ACTTTGAGAAGAAACTTGGTGAGTTTTTCAAGCACCAAATAGAGTCGGATTCTTCGTCGGCATATG GGGATGGGTTCAGGCAAGGTAACCAAGCAGTTCTTACGCACGGTCTGAGGCGAACCCTCGACCATA TTCGCTTGACCGGTACATTTCCTTAA

[1198] DARI -7 protein sequence ( 7 69G>A single base substitution resulting in a Gly257Arg substitution ) ( 2020 -M2 -7 60 Tarvense 15572 ) ( SEQ ID NO : 54 ) :

[1199] ME FLLFFGYLKAVFLFAGKGLLVPMGWLTKILKGSSHKFSDGQVNRRYRDDRNLESSRLSADGSDF DKEEIECAIALSLSEQEHVIPQDDKGKQVIEYKSETEEDEDEDEDEDEEYTRAQLEAAEEEERRAA QAQIEEEEKRRAEAELEE IEKQLAKARLEEEEVRRTKAQLEEDEQLAKAIQESMVVGSPPRYDSGS MFQPFPFLNPSSHRVCTGCLAE IGHGRFLSCMGGVWHPECFCCHACDKPI IDYEFSMSRNRPYHKL CYKEQHHPKCDVCHNFIPTNSAGLIEYRAHPFWMQKYCPSHERDGTPRCCSCERMEPKDTKYLILD DGRKLCLECLDSAIMDTHECQPLYLE IREFYEGLHMKVEQQIPMLLVERSALNEAMEGEKHGHHHL PETRGLCLSEEQTVTTVLRRPRIGAGYKLIDMITEPCRLIRRCEVTAILILYGLPRLLTGSILAHE MMHAWLRLNGYPNLRPEVEEGICQVLAHMWLESETYAGSTLVDIASSSSSSSSAVVTASSKKGERS DFEKKLGEFFKHQIESDSSSAYGDGFRQGNQAVLTHGLRRTLDHIRLTGTFP darl -8 nucleic acid sequence ( 931OT s ingle base substitution resulting in Pro311Ser substitution ) ( 2020-M2 -4 61 Tarvense 15572 ) ( SEQ ID NO : 55 ) :

[1200] ATGGAGTTTCTTCTCTTCTTTGGATACCTTAAAGCAGTGTTTCTCTTTGCAGGTAAAGGATTGTTG GTGCCAATGGGGTGGCTTACTAAAATCCTTAAAGGCTCAAGCCATAAATTCTCGGATGGACAAGTT AACAGAAGATATAGAGATGATAGGAACTTGGAAAGTTCTCGTCTTTCTGCGGTAAGTGTCAGTTTC GTCTTCTGTGTTCTCTGGTTCATCCTTTCTTATTTGAAGAAAGATGTCATTTTGAAGTGAAGGTTA AAGAAAAT GGT AT C T T GG AACT T T GAT T C AGGAT GGAT C TG AT TT T G AC AAAG AAGAGAT T GAAT G T GCT AT TGCACT CT CT CT TTCAGAAC AAGAACAT GT AAT TCCACAAGAT GACAAAGGAAAACAAGT AATCGGTAAGGTTGTCTATTCGCTGGTTGTTTGCATTTGTATAGAGAGAAGAAACAACACACAAGA AAAAGAGAAGAAAGGAAGTTGTGTGAAACTTTATCTTGCTAAGCTGTTTTCTTTTACCTCGTATTC T T T AGAAT AC AAAT CT GAAACC GAAG AAGAT GAGGAT G AGGAT GAGG AC GAAG AT GAAG AAT AC AC GAGAGCTCAGCTAGAAGCAGCGGAAGAAGAGGAAAGACGGGCAGCTCAAGCTCAAATCGAGGAAGA AGAGAAACGGCGAGCTGAAGCTGAATTAGAAGAAATAGAGAAACAGCTTGCGAAAGCTCGACTAGA AGAGGAAGAAGTTAGGCGTACCAAAGCGCAATTGGAGGAAGACGAGCAACTAGCTAAGGCCATTCA AGAAAGTATGGTTGTGGGATCTCCTCCTCGGTATGATTCTGGAAGTATGTTTCAACCATTCCCTTT CCTTAATCCATCTAGTCACAGGTAAATGTTTCAAAAGTTAATTCAATATCCACAACTTAGAATTCA GATACACTGTCATTCTCTTACAATTTTGCTGCAATGAGTATTTTTTCTTTTTCTTTGTTTCTTTAT TGCTTTTGAGGGTATGTACTGGTTGCCTAGCTGAGATTGGACATGGAAGGTTTCTGAGTTGCATGG GTGGCGTTTGGCATCCTGAATGTTTCTGCTGCCATGCTTGTGATAAGCCCATCATAGATTATGAGG TCACAAGACATTTCGTTGAGTAGTGTTACATTGTCCCGAATCTAGTGAATCCTAGCTTGTTAAGTT TTGTTTTTACAGTTCTCAATGTCCGGGAATCGGCCTTATCACAAGCTATGTTACAAGGAGCAGCAT CATCCTAAATGTGATGTTTGCCATAACTTTGTGAGTGATTCCGACCTTTTTATTGATATCATCTTC TGCAAACGATGTTATCAAGTGGTACCAAAGCTTAAATGTGTATGCGTGAAATTAACTCCTATAGAT TCCGACGAATTCAGCTGGTCTCATTGAGTACAGGGCACATCCCTTTTGGATGCAAAAGTATTGTCC TTCACATGAGCGTGATGGAACTTCTCGTTGCTGTAGCTGTGAGCGAATGGAGGTCAGTTTCGATAT GATATATCTACTAATAGGGAAACAATTCTAAGAGACGTTTTTGAGATTTGATGTTGTTCTGTGTGT TTGCTCACCGGTTACAGCCAAAAGATACAAAATATCTCATTCTTGATGATGGTCGAAAACTGTGTC TTGAATGTCTCGACTCAGCCATTATGGACACTCACGAATGCCAACCATTGTACCTTGAGATACGTG AGTTTTATGAAGGCTTACACATGAAAGTAGAACAGCAGATTCCTATGCTCTTGGTGGAGAGATCAG CTTTAAACGAAGCTATGGAAGGAGAGAAACACGTAAAAACCGCTCTTTATTTATCCTATGTTGCTT ACATAAACACTCTTTTTGCTCTTGCTCATTTATTTTTAACTTACTTTCTATTTATTTTTGTTGCAG GGACATCATCATTTACCTGAAACCAGAGGACTCTGTTTGTCTGAGGAACAAACTGTCACAACGGTA AATTGCTCTCACTTTCGTAAGCAGCTATTTCTATCCGCCACAAATTGTGTTTGAATCAGAAAGAGT TTCTTGTAGGTGTTAAGGAGACCAAGAATTGGGGCAGGCTACAAATTGATAGACATGATCACAGAG CCTTGCAGGCTGATACGTCGTTGTGAAGTCACTGCAATTCTCATTCTATATGGACTTCCTCGGTAA AAAAAAAGAATTACTTCCCAACACATTCTTAAACCGACTGTTCTTTCTTGATGAGATAGTTAAAAT ATGTGTATTACAGTTTGTTAACTGGATCAATCCTTGCTCATGAGATGATGCATGCCTGGCTTCGAC TAAATGGTAATTTGAAGAACCCTTCCTTCTTTTCTCGGTACATTAGTAATAATTGCTCTTTGATGT TTTGTTGCTCTCTAAATGTATGAGTAGGGTATCCGAATCTTAGGCCAGAAGTTGAAGAAGGAATAT GTCAGGTTTTGGCTCACATGTGGTTGGAATCTGAGACTTATGCTGGCTCTACATTAGTAGATATCG CATCTTCTTCATCATCATCATCATCGGCTGTGGTCACTGCATCGTCAAAGAAAGGTGAGAGGTCTG ACTTTGAGAAGAAACTTGGTGAGTTTTTCAAGCACCAAATAGAGTCGGATTCTTCGTCGGCATATG GGGATGGGTTCAGGCAAGGTAACCAAGCAGTTCTTACGCACGGTCTGAGGCGAACCCTCGACCATA TTCGCTTGACCGGTACATTTCCTTAA

[1201] DARI-8 protein sequence ( 931OT single base substitution resulting in a Pro311Ser substitution) ( 2020-M2-461 Tarvense 15572 ) ( SEQ ID NO : 56 ) :

[1202] MEFLLFFGYLKAVFLFAGKGLLVPMGWLTKILKGSSHKFSDGQVNRRYRDDRNLESSRLSADGSDF DKEEIECAIALSLSEQEHVIPQDDKGKQVIEYKSETEEDEDEDEDEDEEYTRAQLEAAEEEERRAA QAQIEEEEKRRAEAELEEIEKQLAKARLEEEEVRRTKAQLEEDEQLAKAIQESMVVGSPPRYDSGS MFQPFPFLNPSSHRVCTGCLAEIGHGRFLSCMGGVWHPECFCCHACDKPIIDYEFSMSGNRPYHKL CYKEQHHPKCDVCHNFIPTNSAGLIEYRAHPFWMQKYCPSHERDGTSRCCSCERMEPKDTKYLILD DGRKLCLECLDSAIMDTHECQPLYLEIREFYEGLHMKVEQQIPMLLVERSALNEAMEGEKHGHHHL PETRGLCLSEEQTVTTVLRRPRIGAGYKLIDMITEPCRLIRRCEVTAILILYGLPRLLTGSILAHE MMHAWLRLNGYPNLRPEVEEGICQVLAHMWLESETYAGSTLVDIASSSSSSSSAVVTASSKKGERS DFEKKLGEFFKHQIESDSSSAYGDGFRQGNQAVLTHGLRRTLDHIRLTGTFP upl3-5 nucleic acid sequence ( 4145G>A single base substitution resulting in a Cys l382Tyr substitution ) ( 2020-M2-895 Tarvense 15299 ) ( SEQ ID NO : 57 ) :

[1203] CGATATCGTATGGAGCTTTAATGGAGCAAACGAATCACACGTAGCGGATGGATCCCCGTGTCCAGT TCTTGTCCCAAAAGCCCGAAGGAGCAGGGGAAACGGTATCGTATTCGCTTCATTCTATATTCTGTA CAAAAGCCCTTTTCTCCTCCCTTTTGTTTCATTCCCCCACTCGATTTAGGGTTTTCTTCTAATCCG GTGTGTAAAATTGAAGATCCTTCTTCTCTTCCTTGCTTCCCCCTCTCGATTCCGATCTCATCATCC CTTTTTGATTTCGAAAAATCGTTGATCAGCGTCACGGCTTCTCTATAGCTCCTCCGATCGGCTGGC AAATTAGGGTTTCGTGCGAGCTTAATTCGATCGTTGGACCTGATATGCGAGCGAGTGTCCGATCAG AATCTCTCTGATCGAGAGAGAGCAAATCCCACACTCGGGGTTTCTTCCTTTGTATGGAAACTCGGA GCCGCAAGCGGGCGGAGGCGACCTCAACTGCCCCATCTTCTTCTTCTTCTTCACCTCCTCCTCCCT CAGGCCCCACCACCCGCAGCAAACGCGCTCGTCTTTCCTCTTCCTCTTCCTCCTCTGTTGCCGCTA CCACCACTGCACCTTCCTCTTCCACCCGCTCTCGCTCTTCTCGCTCCGCCGCCGCCGCTGCTACTC CCATGGACACCTCCACCGAGTCTTCTGGATTTCGCCGAGGCGGACGAGGTAACAGGGGTAACGACA ACAGTAATTCTGACAAGGGCAAGGAGAAGGAGCACGACGTTAGGATTAGGGATAGAGAAAGAGAAA GAGACCGAGCCAGAGAACAACTCAACATGGATGCTGCCGCTGCTGCTGCCGCCGCTGACGACGACG ACGACAATGACAGCGAGGATGGCAACGGGGGTTTCATGCATCCCAACATGAGCTCCGCCAGCAGTG CTTTACAAGGTTTGCTGAGGAAGCTTGGAGCTGGCCTCGATGACTTGCTTCCTTCCTCTGGTATCG GCTCTGCTTCTTCTTCCCACTTAAATGGGAGGATGAAGAAGATACTCTCTGGCTTGCGCTCCGAAG GAGAAGAGGGAAAGCAGGTCGAGGCTTTAACCCAGCTATGCGAGATGCTATCCATTGGGACTGAAG ACTCATTAAGCACCTTCTCTGTTGATTCCTTCGTCCCTGTTCTCGTTGGTCTACTAAACCATGAAA GCAATCCCGACATAATGCTTCTTGCGGCCAGGGCTCTTACCCATCTATGTGATGTTTTGCCGTCTT CTTGTGCCGCCGTTGTACATTACGGCGCTGTTTCATGCTTTGTCGCCAGATTGCTAACCATCGAGT ACATGGACTTGGCCGAGCAGGTTGGCTATCCTATCAAAACTTTGAGATTTTGTTGATTTTTATTTA ATTCTAAATTCTGGTGTTTTAATTTGCTCAAGGATCAAGCTTGAAATTTGATTTGTATGCTTAATG TGTTTTACAACAGTCTCTGCAAGCTCTCAAAAAGATATCTCAGGAGCACCCAACTGCCTGTTTGCG AGCTGGCGCTCTGATGGCAGTGCTCTCATATCTTGATTTCTTCTCCACTGGAGTCCAGGTGTGTTA TGTTTGTAACTTTTTCATTTATGCTTTACTTACACCGTTCACGTGTATCTAATGCATCTGTTGCTT CGCTTCTTGTAGCGTGTAGCACTATCTACTGCTGCAAATATGTGCAAGAAATTACCTTCTGATGCA TCTGATTATGTTATGGAAGCTGTACCATTACTGACAAACCTACTTCAGTATCATGATTCGAAGGTA ACTATCCTTCTTTTTGTTGTGATATGGTACTATCTTGTTCCGCTCTTGCCTCAGTTTCCTCCATAA TTTTTTTCGTATGGTGGCAGGTTTTGGAATATGCTTCTATATGTCTGACTCGGATTGCTGAAGCAT TTGCTTCGCACCCTGAGAAATTGGATGAATTATGCAACCATGGCCTTGTGACTCAAGCTGCGTCTC TTATTTCCACTACCAACTCAGGAGGTGGGCAAGCATCTCTGGGCGTTTCAACATACACGGTATGTG TAAATTCTTTTGTGCTTTGTGTATTTATCTTTATTCACAAGATGACATTTTCACCATATTGTGCGC AGGGGTTAATCCGATTACTTTCCACCTGTGCGAGCGGCTCACCTCTTGGATTCAGGACATTGCTTC TTCTCGGCATTAGTAGCATTCTTAAGGATATTCTGTCGGGTCCTGGTGTCTCTGCTAATGCATCTG TATCTCCAGCACTGAGCAGGCCCGCAGATCAGGTATTGATTTTACTTGTTGATATCATAATTGTTT TTTGTTCACTACCTGATTAAAAAATACTAGCTTTTGTATGAATGGTATACAGTGAAATGGTTAGGG ATCGATCACTTGGAGCTGGCATAGATTCTCTATTTCAGCTTTAGTTTCCTCATTTTTATTTGTTTG CCGTATTTTGCGTAGGTGTTTGTATAGCGGATAGATCATGATCTATGTTAAAAAGGTCTTTTGAGC TTTATTTATTTTGGAGGTCTTGTGTTAGAGGTGGTATAGAGAGTTAGAGGTTCATCAATGTGCTTT ATCTGGTTTATTTGTTGTGTAGCCCTTGGTTCGCTAATTCTTGAATTTATTCTTGTTTTCAGAAAT GTTTTGAATTATTACTTTTGTTTTGTATCTTAGATCTTTGAGATAGTCAACCTAGCGAATGAGCTC CTCCCTCCATTGCCAGAAGGAGTTATCTCCCTTCCTACTAGCACAAACACTTTGGTGAAAGGTTCA GGCCAAAAGAAATCTTCTCCAAGTACTTCAGGAAAACAAGACGATTCACCAAAAGTTTCACCTAGA GAAAAATTACTTGCTGATCAACCTGAACTTCTGCAGAAATTTGGACTGGATCTTCTTCCAGTTTTA GTGCAGGTAATTTTTTGCTGCATTTGCTACAAACAATGTTGATACCACCTGCTGTACGTCTAACTA TCCTTGCTTTCTTTCCAACAGATTTATGGTTCTAGTGTCAATGGTACTATTCGCCATAAATGTCTG TCAGTTATCGGAAAGTTGATGTATTTCAGCACTTCAGAAATGATTCAATCTCTAATTGGTGACACA AATATATCGAGGTATGCTGTTTATGATTAAAATTATGTTTTTCATGGCAGTGGGTGCAATGCAACT TCTTACGTTTATTTTTTTGCTATGTAGCTTCTTGGCTGGTGTATTGGCATGGAAAGATCCACAAGT CTTGGTTCCTGCTCTACAAGTTGCAGAAATTCTGATGGAAAAGCTTCCTGAAACATTCTCGAAAGT GTTTGTGAGGGAAGGGGTGGTTCATGCTGTAGATCAACTTGTCTTGGTTGGTAAACCTATTTCTCA TGCTTCACCTACTGATAAGGAAAATGACTGTGTGCCAGGATCTGCACGATCTAGGCGATATAGACG GCGAAGTAGTAATGCCAGTTCTGATGGAAATCAGTCAGAAGAGCTTAAGAATTCTGCGTCGGTTAA CGTAGGTGCAAACCATAATTCCCTAGATTCTCCTACCTCTAGCTTCTTGCTAAAGGAAACAGTTAG CTCCTGTGCCAAAGCATTCAAAGACAAGTACTTCCCGTCTGATGGCGGGGATCTCGATGTTGGAGT TACAGATGATCTCTTACATCTAAAGAATCTTTGCACGAAGCTAACTGCTAGTATAGATGAGCATAA AGTGAAAGGAAAGGGAAAATCCAAAGCCTCTGGGCCATGCCTTGGCGATTTTTCTGCTAGCAAGGA AGAATACTTGATTGGTGTCATTTCTGAGATACTTGGCGAGCTTAGCAAAGGGGATGGAGTCTCAAC TTTTGAGTTTATTGGCAGTGGTGTGGTTGCAGCTTTGCTTGACTATTTCTCTTGTGGGTACTTTTC CAAAGAGAAGATCTCCGAGGTTAATTTGCCCAAACTTCGCCAAGATGGGCTCAGAAGATTCAAAGC

[1204] TTTTCTAGAAGTCGCACTTCCGTCTGATGGTAATGAGGGAAAGATCCCTCCTATGACAGTTTTGAT

[1205] TCAGAAACTTCAAGATGCTTTATCTTCACTGGAACGCTTTCCCGTCGTCCTTAGTCATCCCTCAAG

[1206] GTCATTAGGTGGAAGTGCTCGTCTCTCATCTGGATTGAGTGCTTTGGCACATCCTTTAAAGTTGCG

[1207] ATTATGCCGTGCACCTGGAGAGAAGACACTACGTGATTACTCCTCCAATATTGTTCTTATAGATCC

[1208] ACTGGCAAGCATTGCAGCGGTAGAAGAATTTCTTTGGCCCCGAGTTCAACGGAGTGAATCTGGTAT

[1209] GAAGCATGCAGCGCCTGCTGGAAATACAGAGCCAGGCACGTTACCTAGCGGTGCTGGTGTTTCATC

[1210] ACCATCCTCGTCAACTCCGGCTTCCACCACACGTCGTCATTCTTCTAGATCTAGATCAGCAATTAA

[1211] CATAGGTGATACCTCAAAGAAAGAAGCTGTGCATGAGAAAGGTACCAGCTCATCTAAAGGAAAAGG

[1212] TAAAGGCGTTATGAAGCCCGTTCCAGCTGATAAGGGGCCTCAAACAAGGAGCAGTGCTCAAAGAAG

[1213] AGCTGTCCTTGACAAAGATACACATATGAAACCAGCTAGCGGAGACTCCAGCTCCGAGGTATGTTA

[1214] TTGTACAAAGTTTTGGATTACATGGTTGTTTAATTGTGTTGCATCATATGATATTTGTGGCGTGAT

[1215] CTGCTAATGCACCACTATCTTACCTTTATGCCTTGCAGGATGAGGAATTGGAAATAGCCCCTGTCG

[1216] ACATTGATGATGCCTTGGTGATTGAAGAGGATGACATGTCTGATGATGAAGATGATGATAATGACG

[1217] ATGTAAGTATTCCTTCATATTTGTTTTTTAACCAGTATGTACATTACACTCCGTAGTTATTTCTCT

[1218] TGCTAACAACATGAAAGATGCTACATTGCCAATAACGCTTGATAGCTTCCAAAATTTTGGATTAGT

[1219] TTGCCATGCTGAGACGAACTCATATTGAACATTTCCTTTTAGAATTGACAAAAATAATTACTAACT

[1220] TCATACCACTTCTCTTATTTATCTGGCTGCTATCGGTCTTAATTTCTACAATAACATTACAATCAT

[1221] ATCTATACATTCGTTTCCAGGTGTTGGATGACAGTCTTCCCATGTGCACGCCTGATAAAGTGCATG

[1222] ATGTAAAATTGGGGGACGCAGTGGATGATGACGGTGGTGCTGGTCTGGCCTCTAGCGGGCGACAGA

[1223] TGAATCCAGCTTCAGGTGGCACTAGTGGAGCAGCAGCAGCGAGGGGATCTGATTCTATTGATGCTG

[1224] GCATTGGGAATTCTTACGGTTCTAGGGGTGCACTCTCCTTTGCTGCTGCGGCAATGGCTGGGCTTG

[1225] GAGCTGCCAGTGGTAGAGGTATCAGGGGAAGTAGAGACCTGCATGGGCGTACCCTATATCGAAGTT

[1226] CCGACGAGCCCTCTAAGTTATTATTTACTGCAGGAGGAAAGCAACTTAGTAGGCATTTGACGATAT

[1227] ATCAGGCTGTGCAGCGACAACTTATGCTAGATGAAGACGATGATGAAAGGTTCGGTGGCAGCGATT

[1228] TCATGTCAAGTGATGGAAGCAGATTCAATGATATTTACACCATCATGTACCAGAGGCCGGAGAGTC

[1229] AAGCGAATAGGTTGTCTGTTGGTGCAGCAAGTTCTACCACACTATCAAAATCCACTAAATCTGCTA

[1230] CTGCCAATTCTAGCGTAGAATCTCAGTCATATAAGGCATCTCTTTTGGATAGTATCTTACAAGGGG

[1231] AGCTTCCGTGCGATCTTGAGAAGTCAAATTCTACTTATAATGTTCTGGCGCTGTTGCGTGTATTAG

[1232] AGGGGTTAAATCAGCTTGGCCCTCGGTTGAGAGCCCAAACTGTTTCTGATCGTTTCGCAGAGGGTA

[1233] AAATTACAAGTCTAGATGATCTGAAGACAACTGCTGCTGCTAAGATTTCTCATGAAGAATTCATCA

[1234] ATAGCAAACTTACACCCAAATTAGCTCGACAGATCCAGGATGCGCTTGCTTTGTGCAGTGGTAGTC

[1235] TTCCCTCTTGGTACTACCAGTTGACTAGAGCATGCCCGTTTTTGTTTCCGTTTCAGACCCGGAGAC

[1236] AGTATTTCTATTCAACTGCTTTTGGGTTGTCCCGCGCATTGAACCGCTTGCAGCAGCAGCAAGGTG

[1237] CTGACGGCAGTGGGTCTACAAATGAACGAGAGATGAGAATAGGGAGGTTGCAGCGCCAGAAAGTCC

[1238] GTGTATCCCGAAATAGAATATTAGATTCTGCTGCGAAAGTTATGGAGATGTATTCTAGCCAAAAAG

[1239] CTGTGCTTGAAGTAGAATATTTTGGTGAAGTTGGTACTGGTCTAGGCCCTACACTTGAGTTTTACA

[1240] CACTCCTAAGCCATGATCTACAAAAGGTTGCGCTTGGGATGTGGAGATCAAATTCTGGTGACATGG

[1241] TATCTATGCAAATTGATAGAGGTGAGATTGAAGACGGGAAATCATCCGCAGCTAGGGACAGAGATA

[1242] TAGTTCAGGCACCGCTTGGGTTGTTTCCTCGACCTTGGCCCTCTACAGCTGACGTATCTGAAGGTA

[1243] GTCGGTTTCATAAAGTCATTGAATATTTCCGGCTTTTAGAGGCGTGTGATGGCAAAAGCACTTCAA

[1244] GATGGACGGCTACTGGACGTCCCATTGAGTACAGCGTTTTATAAGCTTATTCTTGGTCAAGTGAGT

[1245] TTTTTTAACTTGGTTTGTTTAGTTGAGATTGAAATAGTTGGTTTTCATTGTTGTTTTATTTATTTA

[1246] TTTCCCCCTCTTATTATTTTTCTCGCTTAAGCAGGAGCTTGATTTGCATGATATCATATTATTTGA

[1247] TACCGAGCTTGGCAAGACTTTGCAAGAGTTTCGTGTTCTTGTTGGCCGCAAGCACTATCTGGAGGC

[1248] AGTAGGCGGTGACAATTGCAGCGCTGGTTCTGATTTATGTTTACGTGGATCCCGTATAGAAGATCT

[1249] CTGCTTGGACTTCACGCTTCCTGGCTATCCTGAATACATTCTGAGACCAGGAGATGAAATTGTACC

[1250] GTCTTATAGCCTACAACCCCACGTCTTACTTTTCTTTTAGTCCCTGTCCATGATTGCTGATGCCGT

[1251] GCTCTTCTGTTACAGGTTGATATGAATAGTCTTGAAGAGTATATATCCCTTGTCGTTGATGCTACT

[1252] GTCAAGAGAGGAGTTACCCGGCAGATTGAAGCCTTTAGATCTGGATTCAATCAGGTTAGCAGTTTC

[1253] GCACACTCTGCTGTCTCTCTCTCTCCCTCTCTCTCTCTTCATGTTGCCTGTGCCTTCTAAATAATA CCGAAAGTGTGGTTTCTGTTGATTAGAGACTTCTTCTGTTTTTGTTTTAGGCTGAGCTCAAATTTT

[1254] TGCCTGCATTTTTTATCTTTACAAAACGTCTTTGTTAATTCACATAATTCTCAGGTGTTTGACGTA

[1255] ACATCTCTACAAATATTCACCCCTTCCGAGCTGGACTATTTGCTGTGTGGTCGTAGAGAGTTGTGG

[1256] GAGGTGATTTTTCTACCTTTTTAGTTTTCCACACCCAGGAGACTTGAATCCCTTACATAGAATTTC

[1257] ATTTCTGAAACCTTTTATTTTTGCAGGCGGAGACTCTTGCTGAACATATCAAGTTTGATCACGGTT

[1258] ATACTGCTAAAAGTCCGGCAATCATTAATGTATGTCAATTTATCCGTTTATATTGAGCAATTAGAA

[1259] ACCATTTCGGTTTACAATCAGCTTTTGCTACTGCACTGGCGATTTTTTTCTGAGTCTTATTTTCAC CCTCACTGTAGTTACTGGAGATCATGGGAGAACTTACAGCGGATCAGCAGAGGGCTTTCTGCCAAT TTGTAACTGGAGCTCCTAGGCTTCCTCCTGGTGGGCTAGCTGTTTTGAATCCAAAGCTGACGATTG TGAGAAAGGTAAAAAATCTTTACTTAATATCTATCTATGCGTGGTCTATGGGCTTTTGGTCTTATT TTCCAAGATCAGACACGCATTGTGGTGTTTTATCAGATATAATATTGGCTCTTTGATTTCGTGAAG CTTTTCTGTAATGGTTCTCAATGGGCTTCTAAAATATTGTTGTCTGCAGCATTCATCAACCTCAAG TGCGGCAGCGAACGGAACAGGGGCTTCGGAGACGGCAGACGATGATCTTCCCAGCGTAATGACTTG CGCCAACTATCTTAAGCTCCCTCCTTATTCGACAAAGGTGAGTCATGTCACTTGTTGTCTTTGAGT CCATCATGTGTTCCGTGAAGTTGTTGTTTGTAAGCCTGAGGACTTGTTGTACCTGAAACAGGAAAT CATGTACAAGAAACTGCTCTACGCCATCAACGAAGGGCAAGGATCGTTCGACCTGTCATAAGCAGA GGCTCCAACTCTCGCTCTCTCCTCTCTCTCTCTCTCTTTGTACATTTACATCGGAAGACGCTGATT TTGATTTTGCTTTTCGGGTTTGAATGATCTGACAAAAGCCGAAGATGCCCCGGGAGACTTGTAATG AGGATATAGAGAATGTGTTGTCTGAAACCTTTGTGGATGTTTTGGTGCATTCCTCTTTGTCTCTTG CAGAAAGAAAAGCTATTTATTGTGTTGTAGATAAAGAAAGATGATAGGCTTATCTTCCCCTTTTAG ATTTCATTTGGTCAGTCAAAAACCATGGTAACTTTAAGTTGGATTACCAGCAAAAACTAGAATATG GACGGCACTTTTATTAAACCATTGAGTTATAAAGGTTAGCTTCAATATTTTTCCTTGTGCCTCCTC CCTCGTATGATTTCTCTCCCA

[1260] UPL3 -5 protein sequence ( 4145G>A s ingle base substitution resulting in a Cys l382Tyr substitution ) ( 2020-M2 - 895 Tarvense 152 99 ) ( SEQ ID NO : 58 ) :

[1261] METRSRKRAEATSTAPSSSSSSPPPPSGPTTRSKRARLSSSSSSSVAATTTAPSSSTRSRSSRSAA

[1262] AAATPMDTSTESSGFRRGGRGNRGNDNSNSDKGKEKEHDVRIRDRERERDRAREQLNMDAAAAAAA

[1263] ADDDDDNDSEDGNGGFMHPNMSSASSALQGLLRKLGAGLDDLLPSSGIGSASSSHLNGRMKKILSG

[1264] LRSEGEEGKQVEALTQLCEMLS IGTEDSLSTFSVDSFVPVLVGLLNHESNPDIMLLAARALTHLCD

[1265] VLPSSCAAVVHYGAVSCFVARLLTIEYMDLAEQSLQALKKI SQEHPTACLRAGALMAVLSYLDFFS

[1266] TGVQRVALSTAANMCKKLPSDASDYVMEAVPLLTNLLQYHDSKVLEYAS ICLTRIAEAFASHPEKL

[1267] DELCNHGLVTQAASLISTTNSGGGQASLGVSTYTGLIRLLSTCASGSPLGFRTLLLLGI SSILKDI

[1268] LSGPGVSANASVSPALSRPADQI FEIVNLANELLPPLPEGVISLPTSTNTLVKGSGQKKSSPSTSG

[1269] KQDDSPKVSPREKLLADQPELLQKFGLDLLPVLVQIYGSSVNGTIRHKCLSVIGKLMYFSTSEMIQ

[1270] SLIGDTNISS FLAGVLAWKDPQVLVPALQVAEILMEKLPET FSKVFVREGVVHAVDQLVLVGKPIS

[1271] HASPTDKENDCVPGSARSRRYRRRSSNASSDGNQSEELKNSASVNVGANHNSLDSPTSS FLLKETV

[1272] SSCAKAFKDKYFPSDGGDLDVGVTDDLLHLKNLCTKLTASIDEHKVKGKGKSKASGPCLGDFSASK

[1273] EEYLIGVISE ILGELSKGDGVSTFEFIGSGVVAALLDY FSCGY FSKEKI SEVNLPKLRQDGLRRFK

[1274] AFLEVALPSDGNEGKIPPMTVLIQKLQDALSSLERFPVVLSHPSRSLGGSARLSSGLSALAHPLKL

[1275] RLCRAPGEKTLRDYSSNIVLIDPLAS IAAVEEFLWPRVQRSESGMKHAAPAGNTEPGTLPSGAGVS

[1276] SPSSSTPASTTRRHSSRSRSAINIGDTSKKEAVHEKGTSSSKGKGKGVMKPVPADKGPQTRSSAQR

[1277] RAVLDKDTHMKPASGDSSSEVLDDSLPMCTPDKVHDVKLGDAVDDDGGAGLASSGRQMNPASGGTS

[1278] GAAAARGSDS IDAGIGNSYGSRGALS FAAAAMAGLGAASGRGIRGSRDLHGRTLYRSSDEPSKLLF

[1279] TAGGKQLSRHLTIYQAVQRQLMLDEDDDERFGGSDFMSSDGSRFNDIYT IMYQRPESQANRLSVGA

[1280] ASSTTLSKSTKSATANSSVESQSYKASLLDSILQGELPCDLEKSNSTYNVLALLRVLEGLNQLGPR

[1281] LRAQTVSDRFAEGKITSLDDLKTTAAAKISHEEFINSKLTPKLARQIQDALALCSGSLPSWYYQLT

[1282] RACPFLFPFQTRRQYFYSTAFGLSRALNRLQQQQGADGSGSTNEREMRIGRLQRQKVRVSRNRILD

[1283] SAAKVMEMYSSQKAVLEVEYFGEVGTGLGPTLEFYTLLSHDLQKVALGMWRSNSGDMVSMQIDRGE

[1284] IEDGKSSAARDRDIVQAPLGLFPRPWPSTADVSEGSRFHKVIEYFRLLGRVMAKALQDGRLLDVPL STAFYKLILGQELDLHDI ILFDTELGKTLQEFRVLVGRKHYLEAVGGDNCSAGSDLCLRGSRIEDL

[1285] CLDFTLPGYPEYILRPGDEIVDMNSLEEYISLVVDATVKRGVTRQIEAFRSGFNQVFDVTSLQIFT PSELDYLLCGRRELWEAETLAEHIKFDHGYTAKSPAIINLLEIMGELTADQQRAFCQFVTGAPRLP PGGLAVLNPKLTIVRKHSSTSSAAANGTGASETADDDLPSVMTCANYLKLPPYSTKEIMYKKLLYA

[1286] INEGQGSFDLS upl3- 6 nucleic acid sequence ( 28G>A single base substitution resulting in a GlulOLys substitution) ( 2020-M2-042 Tarvense 15299 ) ( SEQ ID NO : 59 ) :

[1287] CGATATCGTATGGAGCTTTAATGGAGCAAACGAATCACACGTAGCGGATGGATCCCCGTGTCCAGT TCTTGTCCCAAAAGCCCGAAGGAGCAGGGGAAACGGTATCGTATTCGCTTCATTCTATATTCTGTA CAAAAGCCCTTTTCTCCTCCCTTTTGTTTCATTCCCCCACTCGATTTAGGGTTTTCTTCTAATCCG GTGTGTAAAATTGAAGATCCTTCTTCTCTTCCTTGCTTCCCCCTCTCGATTCCGATCTCATCATCC CTTTTTGATTTCGAAAAATCGTTGATCAGCGTCACGGCTTCTCTATAGCTCCTCCGATCGGCTGGC AAATTAGGGTTTCGTGCGAGCTTAATTCGATCGTTGGACCTGATATGCGAGCGAGTGTCCGATCAG AATCTCTCTGATCGAGAGAGAGCAAATCCCACACTCGGGGTTTCTTCCTTTGTATGGAAACTCGGA GCCGCAAGCGGGCGAAGGCGACCTCAACTGCCCCATCTTCTTCTTCTTCTTCACCTCCTCCTCCCT CAGGCCCCACCACCCGCAGCAAACGCGCTCGTCTTTCCTCTTCCTCTTCCTCCTCTGTTGCCGCTA CCACCACTGCACCTTCCTCTTCCACCCGCTCTCGCTCTTCTCGCTCCGCCGCCGCCGCTGCTACTC CCATGGACACCTCCACCGAGTCTTCTGGATTTCGCCGAGGCGGACGAGGTAACAGGGGTAACGACA ACAGTAATTCTGACAAGGGCAAGGAGAAGGAGCACGACGTTAGGATTAGGGATAGAGAAAGAGAAA GAGACCGAGCCAGAGAACAACTCAACATGGATGCTGCCGCTGCTGCTGCCGCCGCTGACGACGACG ACGACAATGACAGCGAGGATGGCAACGGGGGTTTCATGCATCCCAACATGAGCTCCGCCAGCAGTG CTTTACAAGGTTTGCTGAGGAAGCTTGGAGCTGGCCTCGATGACTTGCTTCCTTCCTCTGGTATCG GCTCTGCTTCTTCTTCCCACTTAAATGGGAGGATGAAGAAGATACTCTCTGGCTTGCGCTCCGAAG GAGAAGAGGGAAAGCAGGTCGAGGCTTTAACCCAGCTATGCGAGATGCTATCCATTGGGACTGAAG ACTCATTAAGCACCTTCTCTGTTGATTCCTTCGTCCCTGTTCTCGTTGGTCTACTAAACCATGAAA GCAATCCCGACATAATGCTTCTTGCGGCCAGGGCTCTTACCCATCTATGTGATGTTTTGCCGTCTT CTTGTGCCGCCGTTGTACATTACGGCGCTGTTTCATGCTTTGTCGCCAGATTGCTAACCATCGAGT ACATGGACTTGGCCGAGCAGGTTGGCTATCCTATCAAAACTTTGAGATTTTGTTGATTTTTATTTA ATTCTAAATTCTGGTGTTTTAATTTGCTCAAGGATCAAGCTTGAAATTTGATTTGTATGCTTAATG TGTTTTACAACAGTCTCTGCAAGCTCTCAAAAAGATATCTCAGGAGCACCCAACTGCCTGTTTGCG AGCTGGCGCTCTGATGGCAGTGCTCTCATATCTTGATTTCTTCTCCACTGGAGTCCAGGTGTGTTA TGTTTGTAACTTTTTCATTTATGCTTTACTTACACCGTTCACGTGTATCTAATGCATCTGTTGCTT CGCTTCTTGTAGCGTGTAGCACTATCTACTGCTGCAAATATGTGCAAGAAATTACCTTCTGATGCA TCTGATTATGTTATGGAAGCTGTACCATTACTGACAAACCTACTTCAGTATCATGATTCGAAGGTA ACTATCCTTCTTTTTGTTGTGATATGGTACTATCTTGTTCCGCTCTTGCCTCAGTTTCCTCCATAA TTTTTTTCGTATGGTGGCAGGTTTTGGAATATGCTTCTATATGTCTGACTCGGATTGCTGAAGCATTTGCTTCGCACCCTGAGAAATTGGATGAATTATGCAACCATGGCCTTGTGACTCAAGCTGCGTCTC TTATTTCCACTACCAACTCAGGAGGTGGGCAAGCATCTCTGGGCGTTTCAACATACACGGTATGTG TAAATTCTTTTGTGCTTTGTGTATTTATCTTTATTCACAAGATGACATTTTCACCATATTGTGCGC AGGGGTTAATCCGATTACTTTCCACCTGTGCGAGCGGCTCACCTCTTGGATTCAGGACATTGCTTC TTCTCGGCATTAGTAGCATTCTTAAGGATATTCTGTCGGGTCCTGGTGTCTCTGCTAATGCATCTG TATCTCCAGCACTGAGCAGGCCCGCAGATCAGGTATTGATTTTACTTGTTGATATCATAATTGTTT TTTGTTCACTACCTGATTAAAAAATACTAGCTTTTGTATGAATGGTATACAGTGAAATGGTTAGGG ATCGATCACTTGGAGCTGGCATAGATTCTCTATTTCAGCTTTAGTTTCCTCATTTTTATTTGTTTG CCGTATTTTGCGTAGGTGTTTGTATAGCGGATAGATCATGATCTATGTTAAAAAGGTCTTTTGAGC TTTATTTATTTTGGAGGTCTTGTGTTAGAGGTGGTATAGAGAGTTAGAGGTTCATCAATGTGCTTT ATCTGGTTTATTTGTTGTGTAGCCCTTGGTTCGCTAATTCTTGAATTTATTCTTGTTTTCAGAAAT GTTTTGAATTATTACTTTTGTTTTGTATCTTAGATCTTTGAGATAGTCAACCTAGCGAATGAGCTC CTCCCTCCATTGCCAGAAGGAGTTATCTCCCTTCCTACTAGCACAAACACTTTGGTGAAAGGTTCA GGCCAAAAGAAATCTTCTCCAAGTACTTCAGGAAAACAAGACGATTCACCAAAAGTTTCACCTAGA

[1288] GAAAAATTACTTGCTGATCAACCTGAACTTCTGCAGAAATTTGGACTGGATCTTCTTCCAGTTTTA

[1289] GTGCAGGTAATTTTTTGCTGCATTTGCTACAAACAATGTTGATACCACCTGCTGTACGTCTAACTA

[1290] TCCTTGCTTTCTTTCCAACAGATTTATGGTTCTAGTGTCAATGGTACTATTCGCCATAAATGTCTG

[1291] TCAGTTATCGGAAAGTTGATGTATTTCAGCACTTCAGAAATGATTCAATCTCTAATTGGTGACACA

[1292] AATATATCGAGGTATGCTGTTTATGATTAAAATTATGTTTTTCATGGCAGTGGGTGCAATGCAACT

[1293] TCTTACGTTTATTTTTTTGCTATGTAGCTTCTTGGCTGGTGTATTGGCATGGAAAGATCCACAAGT

[1294] CTTGGTTCCTGCTCTACAAGTTGCAGAAATTCTGATGGAAAAGCTTCCTGAAACATTCTCGAAAGT

[1295] GTTTGTGAGGGAAGGGGTGGTTCATGCTGTAGATCAACTTGTCTTGGTTGGTAAACCTATTTCTCA

[1296] TGCTTCACCTACTGATAAGGAAAATGACTGTGTGCCAGGATCTGCACGATCTAGGCGATATAGACG

[1297] GCGAAGTAGTAATGCCAGTTCTGATGGAAATCAGTCAGAAGAGCTTAAGAATTCTGCGTCGGTTAA

[1298] CGTAGGTGCAAACCATAATTCCCTAGATTCTCCTACCTCTAGCTTCTTGCTAAAGGAAACAGTTAG

[1299] CTCCTGTGCCAAAGCATTCAAAGACAAGTACTTCCCGTCTGATGGCGGGGATCTCGATGTTGGAGT

[1300] TACAGATGATCTCTTACATCTAAAGAATCTTTGCACGAAGCTAACTGCTAGTATAGATGAGCATAA

[1301] AGTGAAAGGAAAGGGAAAATCCAAAGCCTCTGGGCCATGCCTTGGCGATTTTTCTGCTAGCAAGGA

[1302] AGAATACTTGATTGGTGTCATTTCTGAGATACTTGGCGAGCTTAGCAAAGGGGATGGAGTCTCAAC

[1303] TTTTGAGTTTATTGGCAGTGGTGTGGTTGCAGCTTTGCTTGACTATTTCTCTTGTGGGTACTTTTC

[1304] CAAAGAGAAGATCTCCGAGGTTAATTTGCCCAAACTTCGCCAAGATGGGCTCAGAAGATTCAAAGC

[1305] TTTTCTAGAAGTCGCACTTCCGTCTGATGGTAATGAGGGAAAGATCCCTCCTATGACAGTTTTGAT

[1306] TCAGAAACTTCAAGATGCTTTATCTTCACTGGAACGCTTTCCCGTCGTCCTTAGTCATCCCTCAAG

[1307] GTCATTAGGTGGAAGTGCTCGTCTCTCATCTGGATTGAGTGCTTTGGCACATCCTTTAAAGTTGCG

[1308] ATTATGCCGTGCACCTGGAGAGAAGACACTACGTGATTACTCCTCCAATATTGTTCTTATAGATCC

[1309] ACTGGCAAGCATTGCAGCGGTAGAAGAATTTCTTTGGCCCCGAGTTCAACGGAGTGAATCTGGTAT

[1310] GAAGCATGCAGCGCCTGCTGGAAATACAGAGCCAGGCACGTTACCTAGCGGTGCTGGTGTTTCATC

[1311] ACCATCCTCGTCAACTCCGGCTTCCACCACACGTCGTCATTCTTCTAGATCTAGATCAGCAATTAA

[1312] CATAGGTGATACCTCAAAGAAAGAAGCTGTGCATGAGAAAGGTACCAGCTCATCTAAAGGAAAAGG

[1313] TAAAGGCGTTATGAAGCCCGTTCCAGCTGATAAGGGGCCTCAAACAAGGAGCAGTGCTCAAAGAAG

[1314] AGCTGTCCTTGACAAAGATACACATATGAAACCAGCTAGCGGAGACTCCAGCTCCGAGGTATGTTA

[1315] TTGTACAAAGTTTTGGATTACATGGTTGTTTAATTGTGTTGCATCATATGATATTTGTGGCGTGAT

[1316] CTGCTAATGCACCACTATCTTACCTTTATGCCTTGCAGGATGAGGAATTGGAAATAGCCCCTGTCG

[1317] ACATTGATGATGCCTTGGTGATTGAAGAGGATGACATGTCTGATGATGAAGATGATGATAATGACG

[1318] ATGTAAGTATTCCTTCATATTTGTTTTTTAACCAGTATGTACATTACACTCCGTAGTTATTTCTCT

[1319] TGCTAACAACATGAAAGATGCTACATTGCCAATAACGCTTGATAGCTTCCAAAATTTTGGATTAGT

[1320] TTGCCATGCTGAGACGAACTCATATTGAACATTTCCTTTTAGAATTGACAAAAATAATTACTAACT

[1321] TCATACCACTTCTCTTATTTATCTGGCTGCTATCGGTCTTAATTTCTACAATAACATTACAATCAT

[1322] ATCTATACATTCGTTTCCAGGTGTTGGATGACAGTCTTCCCATGTGCACGCCTGATAAAGTGCATG

[1323] ATGTAAAATTGGGGGACGCAGTGGATGATGACGGTGGTGCTGGTCTGGCCTCTAGCGGGCGACAGA

[1324] TGAATCCAGCTTCAGGTGGCACTAGTGGAGCAGCAGCAGCGAGGGGATCTGATTCTATTGATGCTG

[1325] GCATTGGGAATTCTTACGGTTCTAGGGGTGCACTCTCCTTTGCTGCTGCGGCAATGGCTGGGCTTG

[1326] GAGCTGCCAGTGGTAGAGGTATCAGGGGAAGTAGAGACCTGCATGGGCGTACCCTATATCGAAGTT

[1327] CCGACGAGCCCTCTAAGTTATTATTTACTGCAGGAGGAAAGCAACTTAGTAGGCATTTGACGATAT

[1328] ATCAGGCTGTGCAGCGACAACTTATGCTAGATGAAGACGATGATGAAAGGTTCGGTGGCAGCGATT

[1329] TCATGTCAAGTGATGGAAGCAGATTCAATGATATTTACACCATCATGTACCAGAGGCCGGAGAGTC

[1330] AAGCGAATAGGTTGTCTGTTGGTGCAGCAAGTTCTACCACACTATCAAAATCCACTAAATCTGCTA

[1331] CTGCCAATTCTAGCGTAGAATCTCAGTCATATAAGGCATCTCTTTTGGATAGTATCTTACAAGGGG

[1332] AGCTTCCGTGCGATCTTGAGAAGTCAAATTCTACTTATAATGTTCTGGCGCTGTTGCGTGTATTAG

[1333] AGGGGTTAAATCAGCTTGGCCCTCGGTTGAGAGCCCAAACTGTTTCTGATCGTTTCGCAGAGGGTA

[1334] AAATTACAAGTCTAGATGATCTGAAGACAACTGCTGCTGCTAAGATTTCTCATGAAGAATTCATCA

[1335] ATAGCAAACTTACACCCAAATTAGCTCGACAGATCCAGGATGCGCTTGCTTTGTGCAGTGGTAGTC

[1336] TTCCCTCTTGGTGCTACCAGTTGACTAGAGCATGCCCGTTTTTGTTTCCGTTTCAGACCCGGAGAC

[1337] AGTATTTCTATTCAACTGCTTTTGGGTTGTCCCGCGCATTGAACCGCTTGCAGCAGCAGCAAGGTG CTGACGGCAGTGGGTCTACAAATGAACGAGAGATGAGAATAGGGAGGTTGCAGCGCCAGAAAGTCC

[1338] GTGTATCCCGAAATAGAATATTAGATTCTGCTGCGAAAGTTATGGAGATGTATTCTAGCCAAAAAG

[1339] CTGTGCTTGAAGTAGAATATTTTGGTGAAGTTGGTACTGGTCTAGGCCCTACACTTGAGTTTTACA

[1340] CACTCCTAAGCCATGATCTACAAAAGGTTGCGCTTGGGATGTGGAGATCAAATTCTGGTGACATGG

[1341] TATCTATGCAAATTGATAGAGGTGAGATTGAAGACGGGAAATCATCCGCAGCTAGGGACAGAGATA

[1342] TAGTTCAGGCACCGCTTGGGTTGTTTCCTCGACCTTGGCCCTCTACAGCTGACGTATCTGAAGGTA

[1343] GTCGGTTTCATAAAGTCATTGAATATTTCCGGCTTTTAGGGCGTGTGATGGCAAAAGCACTTCAAG

[1344] ATGGACGGCTACTGGACGTCCCATTGAGTACAGCGTTTTATAAGCTTATTCTTGGTCAAGTGAGTT

[1345] TTTTTAACTTGGTTTGTTTAGTTGAGATTGAAATAGTTGGTTTTCATTGTTGTTTTATTTATTTAT

[1346] TTCCCCCTCTTATTATTTTTCTCGCTTAAGCAGGAGCTTGATTTGCATGATATCATATTATTTGAT

[1347] ACCGAGCTTGGCAAGACTTTGCAAGAGTTTCGTGTTCTTGTTGGCCGCAAGCACTATCTGGAGGCA

[1348] GTAGGCGGTGACAATTGCAGCGCTGGTTCTGATTTATGTTTACGTGGATCCCGTATAGAAGATCTC

[1349] TGCTTGGACTTCACGCTTCCTGGCTATCCTGAATACATTCTGAGACCAGGAGATGAAATTGTACCG

[1350] TCTTATAGCCTACAACCCCACGTCTTACTTTTCTTTTAGTCCCTGTCCATGATTGCTGATGCCGTG

[1351] CTCTTCTGTTACAGGTTGATATGAATAGTCTTGAAGAGTATATATCCCTTGTCGTTGATGCTACTG

[1352] TCAAGAGAGGAGTTACCCGGCAGATTGAAGCCTTTAGATCTGGATTCAATCAGGTTAGCAGTTTCG

[1353] CAGACTCTGCTGTCTCTCTCTCTCCCTCTCTCTCTCTTCATGTTGCCTGTGGCTTCTAAATAATAC

[1354] CGAAAGTGTGGTTTCTGTTGATTAGAGACTTCTTCTGTTTTTGTTTTAGGCTGAGCTCAAATTTTT

[1355] GCCTGCATTTTTTATCTTTACAAAACGTCTTTGTTAATTCACATAATTCTCAGGTGTTTGACGTAA

[1356] CATCTCTACAAATATTCACCCCTTCCGAGCTGGACTATTTGCTGTGTGGTCGTAGAGAGTTGTGGG

[1357] AGGTGATTTTTCTACCTTTTTAGTTTTCCACACCCAGGAGACTTGAATCCCTTACATAGAATTTCA

[1358] TTTCTGAAACCTTTTATTTTTGCAGGCGGAGACTCTTGCTGAACATATCAAGTTTGATCACGGTTA

[1359] TACTGCTAAAAGTCCGGCAATCATTAATGTATGTCAATTTATCCGTTTATATTGAGCAATTAGAAA

[1360] CCATTTCGGTTTACAATCAGCTTTTGCTACTGCACTGGCGATTTTTTTCTGAGTCTTATTTTCACC

[1361] CTCACTGTAGTTACTGGAGATCATGGGAGAACTTACAGCGGATCAGCAGAGGGCTTTCTGCCAATT

[1362] TGTAACTGGAGCTCCTAGGCTTCCTCCTGGTGGGCTAGCTGTTTTGAATCCAAAGCTGACGATTGT

[1363] GAGAAAGGTAAAAAATCTTTACTTAATATCTATCTATGCGTGGTCTATGGGCTTTTGGTCTTATTT

[1364] TCCAAGATCAGACACGCATTGTGGTGTTTTATCAGATATAATATTGGCTCTTTGATTTCGTGAAGC

[1365] TTTTCTGTAATGGTTCTCAATGGGCTTCTAAAATATTGTTGTCTGCAGCATTCATCAACCTCAAGT

[1366] GCGGCAGCGAACGGAACAGGGGCTTCGGAGACGGCAGACGATGATCTTCCCAGCGTAATGACTTGC

[1367] GCCAACTATCTTAAGCTCCCTCCTTATTCGACAAAGGTGAGTCATGTCACTTGTTGTCTTTGAGTC

[1368] CATCATGTGTTCCGTGAAGTTGTTGTTTGTAAGCCTGAGGACTTGTTGTACCTGAAACAGGAAATC

[1369] ATGTACAAGAAACTGCTCTACGCCATCAACGAAGGGCAAGGATCGTTCGACCTGTCATAAGCAGAG GCTCCAACTCTCGCTCTCTCCTCTCTCTCTCTCTCTTTGTACATTTACATCGGAAGACGCTGATTT TGATTTTGCTTTTCGGGTTTGAATGATCTGACAAAAGCCGAAGATGCCCCGGGAGACTTGTAATGA

[1370] GGATATAGAGAATGTGTTGTCTGAAACCTTTGTGGATGTTTTGGTGCATTCCTCTTTGTCTCTTGC

[1371] AGAAAGAAAAGCTATTTATTGTGTTGTAGATAAAGAAAGATGATAGGCTTATCTTCCCCTTTTAGA TTTCATTTGGTCAGTCAAAAACCATGGTAACTTTAAGTTGGATTACCAGCAAAAACTAGAATATGG ACGGCACTTTTATTAAACCATTGAGTTATAAAGGTTAGCTTCAATATTTTTCCTTGTGCCTCCTCC

[1372] CTCGTATGATTTCTCTCCCA

[1373] UPL3-6 protein sequence ( 28G>A single base substitution resulting in a Glul OLys substitution) ( 2020-M2-042 Tarvense 15299 ) ( SEQ ID

[1374] NO : 60 ) :

[1375] METRSRKRAKATSTAPSSSSSSPPPPSGPTTRSKRARLSSSSSSSVAATTTAPSSSTRSRSSRSAA

[1376] AAATPMDTSTESSGFRRGGRGNRGNDNSNSDKGKEKEHDVRIRDRERERDRAREQLNMDAAAAAAA

[1377] ADDDDDNDSEDGNGGFMHPNMSSASSALQGLLRKLGAGLDDLLPSSGIGSASSSHLNGRMKKILSG

[1378] LRSEGEEGKQVEALTQLCEMLSIGTEDSLSTFSVDSFVPVLVGLLNHESNPDIMLLAARALTHLCD

[1379] VLPSSCAAVVHYGAVSCFVARLLTIEYMDLAEQSLQALKKISQEHPTACLRAGALMAVLSYLDFFS

[1380] TGVQRVALSTAANMCKKLPSDASDYVMEAVPLLTNLLQYHDSKVLEYASICLTRIAEAFASHPEKL

[1381] DELCNHGLVTQAASLISTTNSGGGQASLGVSTYTGLIRLLSTCASGSPLGFRTLLLLGISSILKDI LSGPGVSANASVSPALSRPADQI FEIVNLANELLPPLPEGVISLPTSTNTLVKGSGQKKSSPSTSG

[1382] KQDDSPKVSPREKLLADQPELLQKFGLDLLPVLVQIYGSSVNGTIRHKCLSVIGKLMYFSTSEMIQ

[1383] SLIGDTNISS FLAGVLAWKDPQVLVPALQVAEILMEKLPET FSKVFVREGVVHAVDQLVLVGKPIS

[1384] HASPTDKENDCVPGSARSRRYRRRSSNASSDGNQSEELKNSASVNVGANHNSLDSPTSS FLLKETV

[1385] SSCAKAFKDKYFPSDGGDLDVGVTDDLLHLKNLCTKLTASIDEHKVKGKGKSKASGPCLGDFSASK

[1386] EEYLIGVISE ILGELSKGDGVSTFEFIGSGVVAALLDY FSCGY FSKEKI SEVNLPKLRQDGLRRFK

[1387] AFLEVALPSDGNEGKIPPMTVLIQKLQDALSSLERFPVVLSHPSRSLGGSARLSSGLSALAHPLKL

[1388] RLCRAPGEKTLRDYSSNIVLIDPLAS IAAVEEFLWPRVQRSESGMKHAAPAGNTEPGTLPSGAGVS

[1389] SPSSSTPASTTRRHSSRSRSAINIGDTSKKEAVHEKGTSSSKGKGKGVMKPVPADKGPQTRSSAQR

[1390] RAVLDKDTHMKPASGDSSSEVLDDSLPMCTPDKVHDVKLGDAVDDDGGAGLASSGRQMNPASGGTS

[1391] GAAAARGSDS IDAGIGNSYGSRGALS FAAAAMAGLGAASGRGIRGSRDLHGRTLYRSSDEPSKLLF

[1392] TAGGKQLSRHLTIYQAVQRQLMLDEDDDERFGGSDFMSSDGSRFNDIYT IMYQRPESQANRLSVGA

[1393] ASSTTLSKSTKSATANSSVESQSYKASLLDSILQGELPCDLEKSNSTYNVLALLRVLEGLNQLGPR

[1394] LRAQTVSDRFAEGKITSLDDLKTTAAAKISHEEFINSKLTPKLARQIQDALALCSGSLPSWCYQLT

[1395] RACPFLFPFQTRRQYFYSTAFGLSRALNRLQQQQGADGSGSTNEREMRIGRLQRQKVRVSRNRILD

[1396] SAAKVMEMYSSQKAVLEVEYFGEVGTGLGPTLEFYTLLSHDLQKVALGMWRSNSGDMVSMQIDRGE

[1397] IEDGKSSAARDRDIVQAPLGLFPRPWPSTADVSEGSRFHKVIEYFRLLGRVMAKALQDGRLLDVPL

[1398] STAFYKLILGQELDLHDI ILFDTELGKTLQEFRVLVGRKHYLEAVGGDNCSAGSDLCLRGSRIEDL

[1399] CLDFTLPGYPEYILRPGDEIVDMNSLEEYI SLVVDATVKRGVTRQIEAFRSGFNQVFDVTSLQI FT

[1400] PSELDYLLCGRRELWEAETLAEHIKFDHGYTAKSPAI INLLEIMGELTADQQRAFCQFVTGAPRLP

[1401] PGGLAVLNPKLTIVRKHSSTSSAAANGTGASETADDDLPSVMTCANYLKLPPYSTKE IMYKKLLYA INEGQGSFDLS upl3- 7 nucleic acid sequence ( 5515G>A s ingle base substitution resulting in a Glul 839Lys substitution ) ( 2020 -M2- 005 Tarvense 15299 ) ( SEQ ID NO : 61 ) :

[1402] CGATATCGTATGGAGCTTTAATGGAGCAAACGAATCACACGTAGCGGATGGATCCCCGTGTCCAGT

[1403] TCTTGTCCCAAAAGCCCGAAGGAGCAGGGGAAACGGTATCGTATTCGCTTCATTCTATATTCTGTA

[1404] CAAAAGCCCTTTTCTCCTCCCTTTTGTTTCATTCCCCCACTCGATTTAGGGTTTTCTTCTAATCCG

[1405] GTGTGTAAAATTGAAGATCCTTCTTCTCTTCCTTGCTTCCCCCTCTCGATTCCGATCTCATCATCC

[1406] CTTTTTGATTTCGAAAAATCGTTGATCAGCGTCACGGCTTCTCTATAGCTCCTCCGATCGGCTGGC

[1407] AAATTAGGGTTTCGTGCGAGCTTAATTCGATCGTTGGACCTGATATGCGAGCGAGTGTCCGATCAG

[1408] AATCTCTCTGATCGAGAGAGAGCAAATCCCACACTCGGGGTTTCTTCCTTTGTATGGAAACTCGGA

[1409] GCCGCAAGCGGGCGGAGGCGACCTCAACTGCCCCATCTTCTTCTTCTTCTTCACCTCCTCCTCCCT

[1410] CAGGCCCCACCACCCGCAGCAAACGCGCTCGTCTTTCCTCTTCCTCTTCCTCCTCTGTTGCCGCTA

[1411] CCACCACTGCACCTTCCTCTTCCACCCGCTCTCGCTCTTCTCGCTCCGCCGCCGCCGCTGCTACTC

[1412] CCATGGACACCTCCACCGAGTCTTCTGGATTTCGCCGAGGCGGACGAGGTAACAGGGGTAACGACA

[1413] ACAGTAATTCTGACAAGGGCAAGGAGAAGGAGCACGACGTTAGGATTAGGGATAGAGAAAGAGAAA

[1414] GAGACCGAGCCAGAGAACAACTCAACATGGATGCTGCCGCTGCTGCTGCCGCCGCTGACGACGACG

[1415] ACGACAATGACAGCGAGGATGGCAACGGGGGTTTCATGCATCCCAACATGAGCTCCGCCAGCAGTG

[1416] CTTTACAAGGTTTGCTGAGGAAGCTTGGAGCTGGCCTCGATGACTTGCTTCCTTCCTCTGGTATCGGCTCTGCTTCTTCTTCCCACTTAAATGGGAGGATGAAGAAGATACTCTCTGGCTTGCGCTCCGAAGGAGAAGAGGGAAAGCAGGTCGAGGCTTTAACCCAGCTATGCGAGATGCTATCCATTGGGACTGAAG

[1417] ACTCATTAAGCACCTTCTCTGTTGATTCCTTCGTCCCTGTTCTCGTTGGTCTACTAAACCATGAAA

[1418] GCAATCCCGACATAATGCTTCTTGCGGCCAGGGCTCTTACCCATCTATGTGATGTTTTGCCGTCTT

[1419] CTTGTGCCGCCGTTGTACATTACGGCGCTGTTTCATGCTTTGTCGCCAGATTGCTAACCATCGAGT

[1420] ACATGGACTTGGCCGAGCAGGTTGGCTATCCTATCAAAACTTTGAGATTTTGTTGATTTTTATTTA

[1421] ATTCTAAATTCTGGTGTTTTAATTTGCTCAAGGATCAAGCTTGAAATTTGATTTGTATGCTTAATG

[1422] TGTTTTACAACAGTCTCTGCAAGCTCTCAAAAAGATATCTCAGGAGCACCCAACTGCCTGTTTGCG

[1423] AGCTGGCGCTCTGATGGCAGTGCTCTCATATCTTGATTTCTTCTCCACTGGAGTCCAGGTGTGTTA

[1424] TGTTTGTAACTTTTTCATTTATGCTTTACTTACACCCTTCACGTCTATCTAATGCATCTGTTGCTT CGCTTCTTGTAGCGTGTAGCACTATCTACTGCTGCAAATATGTGCAAGAAATTACCTTCTGATGCA TCTGATTATGTTATGGAAGCTGTACCATTACTGACAAACCTACTTCAGTATCATGATTCGAAGGTA ACTATCCTTCTTTTTGTTGTGATATGGTACTATCTTGTTCCGCTCTTGCCTCAGTTTCCTCCATAA TTTTTTTCGTATGGTGGCAGGTTTTGGAATATGCTTCTATATGTCTGACTCGGATTGCTGAAGCAT TTGCTTCGCACCCTGAGAAATTGGATGAATTATGCAACCATGGCCTTGTGACTCAAGCTGCGTCTC TTATTTCCACTACCAACTCAGGAGGTGGGCAAGCATCTCTGGGCGTTTCAACATACACGGTATGTG TAAATTCTTTTGTGCTTTGTGTATTTATCTTTATTCACAAGATGACATTTTCACCATATTGTGCGC AGGGGTTAATCCGATTACTTTCCACCTGTGCGAGCGGCTCACCTCTTGGATTCAGGACATTGCTTC TTCTCGGCATTAGTAGCATTCTTAAGGATATTCTGTCGGGTCCTGGTGTCTCTGCTAATGCATCTG TATCTCCAGCACTGAGCAGGCCCGCAGATCAGGTATTGATTTTACTTGTTGATATCATAATTGTTT TTTGTTCACTACCTGATTAAAAAATACTAGCTTTTGTATGAATGGTATACAGTGAAATGGTTAGGG ATCGATCACTTGGAGCTGGCATAGATTCTCTATTTCAGCTTTAGTTTCCTCATTTTTATTTGTTTG CCGTATTTTGCGTAGGTGTTTGTATAGCGGATAGATCATGATCTATGTTAAAAAGGTCTTTTGAGC TTTATTTATTTTGGAGGTCTTGTGTTAGAGGTGGTATAGAGAGTTAGAGGTTCATCAATGTGCTTT ATCTGGTTTATTTGTTGTGTAGCCCTTGGTTCGCTAATTCTTGAATTTATTCTTGTTTTCAGAAAT GTTTTGAATTATTACTTTTGTTTTGTATCTTAGATCTTTGAGATAGTCAACCTAGCGAATGAGCTC CTCCCTCCATTGCCAGAAGGAGTTATCTCCCTTCCTACTAGCACAAACACTTTGGTGAAAGGTTCA GGCCAAAAGAAATCTTCTCCAAGTACTTCAGGAAAACAAGACGATTCACCAAAAGTTTCACCTAGA GAAAAATTACTTGCTGATCAACCTGAACTTCTGCAGAAATTTGGACTGGATCTTCTTCCAGTTTTA GTGCAGGTAATTTTTTGCTGCATTTGCTACAAACAATGTTGATACCACCTGCTGTACGTCTAACTA TCCTTGCTTTCTTTCCAACAGATTTATGGTTCTAGTGTCAATGGTACTATTCGCCATAAATGTCTG TCAGTTATCGGAAAGTTGATGTATTTCAGCACTTCAGAAATGATTCAATCTCTAATTGGTGACACA AATATATCGAGGTATGCTGTTTATGATTAAAATTATGTTTTTCATGGCAGTGGGTGCAATGCAACT TCTTACGTTTATTTTTTTGCTATGTAGCTTCTTGGCTGGTGTATTGGCATGGAAAGATCCACAAGT CTTGGTTCCTGCTCTACAAGTTGCAGAAATTCTGATGGAAAAGCTTCCTGAAACATTCTCGAAAGT GTTTGTGAGGGAAGGGGTGGTTCATGCTGTAGATCAACTTGTCTTGGTTGGTAAACCTATTTCTCA TGCTTCACCTACTGATAAGGAAAATGACTGTGTGCCAGGATCTGCACGATCTAGGCGATATAGACG GCGAAGTAGTAATGCCAGTTCTGATGGAAATCAGTCAGAAGAGCTTAAGAATTCTGCGTCGGTTAA CGTAGGTGCAAACCATAATTCCCTAGATTCTCCTACCTCTAGCTTCTTGCTAAAGGAAACAGTTAG CTCCTGTGCCAAAGCATTCAAAGACAAGTACTTCCCGTCTGATGGCGGGGATCTCGATGTTGGAGT TACAGATGATCTCTTACATCTAAAGAATCTTTGCACGAAGCTAACTGCTAGTATAGATGAGCATAA AGTGAAAGGAAAGGGAAAATCCAAAGCCTCTGGGCCATGCCTTGGCGATTTTTCTGCTAGCAAGGA AGAATACTTGATTGGTGTCATTTCTGAGATACTTGGCGAGCTTAGCAAAGGGGATGGAGTCTCAAC TTTTGAGTTTATTGGCAGTGGTGTGGTTGCAGCTTTGCTTGACTATTTCTCTTGTGGGTACTTTTC CAAAGAGAAGATCTCCGAGGTTAATTTGCCCAAACTTCGCCAAGATGGGCTCAGAAGATTCAAAGC TTTTCTAGAAGTCGCACTTCCGTCTGATGGTAATGAGGGAAAGATCCCTCCTATGACAGTTTTGAT TCAGAAACTTCAAGATGCTTTATCTTCACTGGAACGCTTTCCCGTCGTCCTTAGTCATCCCTCAAG GTCATTAGGTGGAAGTGCTCGTCTCTCATCTGGATTGAGTGCTTTGGCACATCCTTTAAAGTTGCG ATTATGCCGTGCACCTGGAGAGAAGACACTACGTGATTACTCCTCCAATATTGTTCTTATAGATCC ACTGGCAAGCATTGCAGCGGTAGAAGAATTTCTTTGGCCCCGAGTTCAACGGAGTGAATCTGGTAT GAAGCATGCAGCGCCTGCTGGAAATACAGAGCCAGGCACGTTACCTAGCGGTGCTGGTGTTTCATC ACCATCCTCGTCAACTCCGGCTTCCACCACACGTCGTCATTCTTCTAGATCTAGATCAGCAATTAA CATAGGTGATACCTCAAAGAAAGAAGCTGTGCATGAGAAAGGTACCAGCTCATCTAAAGGAAAAGG TAAAGGCGTTATGAAGCCCGTTCCAGCTGATAAGGGGCCTCAAACAAGGAGCAGTGCTCAAAGAAG AGCTGTCCTTGACAAAGATACACATATGAAACCAGCTAGCGGAGACTCCAGCTCCGAGGTATGTTA TTGTACAAAGTTTTGGATTACATGGTTGTTTAATTGTGTTGCATCATATGATATTTGTGGCGTGAT CTGCTAATGCACCACTATCTTACCTTTATGCCTTGCAGGATGAGGAATTGGAAATAGCCCCTGTCG ACATTGATGATGCCTTGGTGATTGAAGAGGATGACATGTCTGATGATGAAGATGATGATAATGACG ATGTAAGTATTCCTTCATATTTGTTTTTTAACCAGTATGTACATTACACTCCGTAGTTATTTCTCT TGCTAACAACATGAAAGATGCTACATTGCCAATAACGCTTGATAGCTTCCAAAATTTTGGATTAGT TTGCCATGCTGAGACGAACTCATATTGAACATTTCCTTTTAGAATTGACAAAAATAATTACTAACT TCATACCACTTCTCTTATTTATCTGGCTGCTATCGGTCTTAATTTCTACZkATAACATTACAATCAT

[1425] ATCTATACATTCGTTTCCAGGTGTTGGATGACAGTCTTCCCATGTGCACGCCTGATAAAGTGCATG

[1426] ATGTAAAATTGGGGGACGCAGTGGATGATGACGGTGGTGCTGGTCTGGCCTCTAGCGGGCGACAGA

[1427] TGAATCCAGCTTCAGGTGGCACTAGTGGAGCAGCAGCAGCGAGGGGATCTGATTCTATTGATGCTG

[1428] GCATTGGGAATTCTTACGGTTCTAGGGGTGCACTCTCCTTTGCTGCTGCGGCAATGGCTGGGCTTG

[1429] GAGCTGCCAGTGGTAGAGGTATCAGGGGAAGTAGAGACCTGCATGGGCGTACCCTATATCGAAGTT

[1430] CCGACGAGCCCTCTAAGTTATTATTTACTGCAGGAGGAAAGCAACTTAGTAGGCATTTGACGATAT

[1431] ATCAGGCTGTGCAGCGACAACTTATGCTAGATGAAGACGATGATGAAAGGTTCGGTGGCAGCGATT

[1432] TCATGTCAAGTGATGGAAGCAGATTCAATGATATTTACACCATCATGTACCAGAGGCCGGAGAGTC

[1433] AAGCGAATAGGTTGTCTGTTGGTGCAGCAAGTTCTACCACACTATCAAAATCCACTAAATCTGCTA

[1434] CTGCCAATTCTAGCGTAGAATCTCAGTCATATAAGGCATCTCTTTTGGATAGTATCTTACAAGGGG

[1435] AGCTTCCGTGCGATCTTGAGAAGTCAAATTCTACTTATAATGTTCTGGCGCTGTTGCGTGTATTAG

[1436] AGGGGTTAAATCAGCTTGGCCCTCGGTTGAGAGCCCAAACTGTTTCTGATCGTTTCGCAGAGGGTA

[1437] AAATTACAAGTCTAGATGATCTGAAGACAACTGCTGCTGCTAAGATTTCTCATGAAGAATTCATCA

[1438] ATAGCAAACTTACACCCAAATTAGCTCGACAGATCCAGGATGCGCTTGCTTTGTGCAGTGGTAGTC

[1439] TTCCCTCTTGGTGCTACCAGTTGACTAGAGCATGCCCGTTTTTGTTTCCGTTTCAGACCCGGAGAC

[1440] AGTATTTCTATTCAACTGCTTTTGGGTTGTCCCGCGCATTGAACCGCTTGCAGCAGCAGCAAGGTG

[1441] CTGACGGCAGTGGGTCTACAAATGAACGAGAGATGAGAATAGGGAGGTTGCAGCGCCAGAAAGTCC

[1442] GTGTATCCCGAAATAGAATATTAGATTCTGCTGCGAAAGTTATGGAGATGTATTCTAGCCAAAAAG

[1443] CTGTGCTTGAAGTAGAATATTTTGGTGAAGTTGGTACTGGTCTAGGCCCTACACTTGAGTTTTACA

[1444] CACTCCTAAGCCATGATCTACAAAAGGTTGCGCTTGGGATGTGGAGATCAAATTCTGGTGACATGG

[1445] TATCTATGCAAATTGATAGAGGTGAGATTGAAGACGGGAAATCATCCGCAGCTAGGGACAGAGATA

[1446] TAGTTCAGGCACCGCTTGGGTTGTTTCCTCGACCTTGGCCCTCTACAGCTGACGTATCTGAAGGTA

[1447] GTCGGTTTCATAAAGTCATTGAATATTTCCGGCTTTTAGGGCGTGTGATGGCAAAAGCACTTCAAG

[1448] ATGGACGGCTACTGGACGTCCCATTGAGTACAGCGTTTTATAAGCTTATTCTTGGTCAAGTGAGTT

[1449] TTTTTAACTTGGTTTGTTTAGTTGAGATTGAAATAGTTGGTTTTCATTGTTGTTTTATTTATTTAT

[1450] TTCCCCCTCTTATTATTTTTCTCGCTTAAGCAGGAGCTTGATTTGCATGATATCATATTATTTGAT

[1451] ACCGAGCTTGGCAAGACTTTGCAAGAGTTTCGTGTTCTTGTTGGCCGCAAGCACTATCTGGAGGCA

[1452] GTAGGCGGTGACAATTGCAGCGCTGGTTCTGATTTATGTTTACGTGGATCCCGTATAGAAGATCTC

[1453] TGCTTGGACTTCACGCTTCCTGGCTATCCTGAATACATTCTGAGACCAGGAGATGAAATTGTACCG

[1454] TCTTATAGCCTACAACCCCACGTCTTACTTTTCTTTTAGTCCCTGTCCATGATTGCTGATGCCGTG

[1455] CTCTTCTGTTACAGGTTGATATGAATAGTCTTGAAGAGTATATATCCCTTGTCGTTGATGCTACTG

[1456] TCAAGAGAGGAGTTACCCGGCAGATTGAAGCCTTTAGATCTGGATTCAATCAGGTTAGCAGTTTCG

[1457] CAGACTCTGCTGTCTCTCTCTCTCCCTCTCTCTCTCTTCATGTTGCCTGTGGCTTCTAAATAATAC

[1458] CGAAAGTGTGGTTTCTGTTGATTAGAGACTTCTTCTGTTTTTGTTTTAGGCTGAGCTCAAATTTTT

[1459] GCCTGCATTTTTTATCTTTACAAAACGTCTTTGTTAATTCACATAATTCTCAGGTGTTTGACGTAA

[1460] CATCTCTACAAATATTCACCCCTTCCGAGCTGGACTATTTGCTGTGTGGTCGTAGAGAGTTGTGGG

[1461] AGGTGATTTTTCTACCTTTTTAGTTTTCCACACCCAGGAGACTTGAATCCCTTACATAGAATTTCA

[1462] TTTCTGAAACCTTTTATTTTTGCAGGCGGAGACTCTTGCTGAACATATCAAGTTTGATCACGGTTA

[1463] TACTGCTAAAAGTCCGGCAATCATTAATGTATGTCAATTTATCCGTTTATATTGAGCAATTAGAAA

[1464] CCATTTCGGTTTACAATCAGCTTTTGCTACTGCACTGGCGATTTTTTTCTGAGTCTTATTTTCACC

[1465] CTCACTGTAGTTACTGGAGATCATGGGAGAACTTACAGCGGATCAGCAGAGGGCTTTCTGCCAATT

[1466] TGTAACTGGAGCTCCTAGGCTTCCTCCTGGTGGGCTAGCTGTTTTGAATCCAAAGCTGACGATTGT

[1467] GAGAAAGGTAAAAAATCTTTACTTAATATCTATCTATGCGTGGTCTATGGGCTTTTGGTCTTATTT

[1468] TCCAAGATCAGACACGCATTGTGGTGTTTTATCAGATATAATATTGGCTCTTTGATTTCGTGAAGC

[1469] TTTTCTGTAATGGTTCTCAATGGGCTTCTAAAATATTGTTGTCTGCAGCATTCATCAACCTCAAGT

[1470] GCGGCAGCGAACGGAACAGGGGCTTCGGAGACGGCAGACGATGATCTTCCCAGCGTAATGACTTGC

[1471] GCCAACTATCTTAAGCTCCCTCCTTATTCGACAAAGGTGAGTCATGTCACTTGTTGTCTTTGAGTC

[1472] CATCATGTGTTCCGTGAAGTTGTTGTTTGTAAGCCTGAGGACTTGTTGTACCTGAAACAGAAAATC

[1473] ATGTACAAGAAACTGCTCTACGCCATCAACGAAGGGCAAGGATCGTTCGACCTGTCATAAGCAGAG

[1474] GCTCCAACTCTCGCTCTCTCCTCTCTCTCTCTCTCTTTGTACATTTACATCGGAAGACGCTGATTT TGATTTTGCTTTTCGGGTTTGAATGATCTGACAAAAGCCGAAGATGCCCCGGGAGACTTGTAATGA GGATATAGAGAATGTGTTGTCTGAAACCTTTGTGGATGTTTTGGTGCATTCCTCTTTGTCTCTTGC AGAAAGAAAAGCTATTTATTGTGTTGTAGATAAAGAAAGATGATAGGCTTATCTTCCCCTTTTAGA TTTCATTTGGTCAGTCAAAAACCATGGTAACTTTAAGTTGGATTACCAGCAAAAACTAGAATATGG ACGGCACTTTTATTAAACCATTGAGTTATAAAGGTTAGCTTCAATATTTTTCCTTGTGCCTCCTCC CTCGTATGATTTCTCTCCCA

[1475] UPL3 -7 protein sequence ( 5515G>A s ingle base substitution resulting in a Glul 839Lys substitution ) ( 2020-M2 - 005 Tarvense 152 99 ) ( SEQ ID NO : 62 ) :

[1476] METRSRKRAEATSTAPSSSSSSPPPPSGPTTRSKRARLSSSSSSSVAATTTAPSSSTRSRSSRSAA AAATPMDTSTESSGFRRGGRGNRGNDNSNSDKGKEKEHDVRIRDRERERDRAREQLNMDAAAAAAA ADDDDDNDSEDGNGGFMHPNMSSASSALQGLLRKLGAGLDDLLPSSGIGSASSSHLNGRMKKILSG LRSEGEEGKQVEALTQLCEMLS IGTEDSLSTFSVDSFVPVLVGLLNHESNPDIMLLAARALTHLCD VLPSSCAAVVHYGAVSCFVARLLTIEYMDLAEQSLQALKKI SQEHPTACLRAGALMAVLSYLDFFS TGVQRVALSTAANMCKKLPSDASDYVMEAVPLLTNLLQYHDSKVLEYAS ICLTRIAEAFASHPEKL DELCNHGLVTQAASLISTTNSGGGQASLGVSTYTGLIRLLSTCASGSPLGFRTLLLLGI SSILKDI LSGPGVSANASVSPALSRPADQI FEIVNLANELLPPLPEGVISLPTSTNTLVKGSGQKKSSPSTSG KQDDSPKVSPREKLLADQPELLQKFGLDLLPVLVQIYGSSVNGTIRHKCLSVIGKLMYFSTSEMIQ SLIGDTNISS FLAGVLAWKDPQVLVPALQVAEILMEKLPET FSKVFVREGVVHAVDQLVLVGKPIS HASPTDKENDCVPGSARSRRYRRRSSNASSDGNQSEELKNSASVNVGANHNSLDSPTSS FLLKETV SSCAKAFKDKYFPSDGGDLDVGVTDDLLHLKNLCTKLTASIDEHKVKGKGKSKASGPCLGDFSASK EEYLIGVISE ILGELSKGDGVSTFEFIGSGVVAALLDY FSCGY FSKEKI SEVNLPKLRQDGLRRFK AFLEVALPSDGNEGKIPPMTVLIQKLQDALSSLERFPVVLSHPSRSLGGSARLSSGLSALAHPLKL RLCRAPGEKTLRDYSSNIVLIDPLAS IAAVEEFLWPRVQRSESGMKHAAPAGNTEPGTLPSGAGVS SPSSSTPASTTRRHSSRSRSAINIGDTSKKEAVHEKGTSSSKGKGKGVMKPVPADKGPQTRSSAQR RAVLDKDTHMKPASGDSSSEVLDDSLPMCTPDKVHDVKLGDAVDDDGGAGLASSGRQMNPASGGTS GAAAARGSDS IDAGIGNSYGSRGALS FAAAAMAGLGAASGRGIRGSRDLHGRTLYRSSDEPSKLLF TAGGKQLSRHLTIYQAVQRQLMLDEDDDERFGGSDFMSSDGSRFNDIYT IMYQRPESQANRLSVGA ASSTTLSKSTKSATANSSVESQSYKASLLDSILQGELPCDLEKSNSTYNVLALLRVLEGLNQLGPR LRAQTVSDRFAEGKITSLDDLKTTAAAKISHEEFINSKLTPKLARQIQDALALCSGSLPSWCYQLT RACPFLFPFQTRRQYFYSTAFGLSRALNRLQQQQGADGSGSTNEREMRIGRLQRQKVRVSRNRILD SAAKVMEMYSSQKAVLEVEYFGEVGTGLGPTLEFYTLLSHDLQKVALGMWRSNSGDMVSMQIDRGE IEDGKSSAARDRDIVQAPLGLFPRPWPSTADVSEGSRFHKVIEYFRLLGRVMAKALQDGRLLDVPL STAFYKLILGQELDLHDI ILFDTELGKTLQEFRVLVGRKHYLEAVGGDNCSAGSDLCLRGSRIEDL CLDFTLPGYPEYILRPGDEIVDMNSLEEYI SLVVDATVKRGVTRQIEAFRSGFNQVFDVTSLQI FT PSELDYLLCGRRELWEAETLAEHIKFDHGYTAKSPAI INLLEIMGELTADQQRAFCQFVTGAPRLP PGGLAVLNPKLTIVRKHSSTSSAAANGTGASETADDDLPSVMTCANYLKLPPYSTKKIMYKKLLYA INEGQGSFDLS upl3-8 nucleic acid sequence ( 922G>A s ingle base substitution resulting in a Glu308Lys substitution ) ( 2019-M2 -102 Tarvense 15299 ) ( SEQ ID NO : 63 ) :

[1477] CGATATCGTATGGAGCTTTAATGGAGCAAACGAATCACACGTAGCGGATGGATCCCCGTGTCCAGT TCTTGTCCCAAAAGCCCGAAGGAGCAGGGGAAACGGTATCGTATTCGCTTCATTCTATATTCTGTA CAAAAGCCCTTTTCTCCTCCCTTTTGTTTCATTCCCCCACTCGATTTAGGGTTTTCTTCTAATCCG GTGTGTAAAATTGAAGATCCTTCTTCTCTTCCTTGCTTCCCCCTCTCGATTCCGATCTCATCATCC CTTTTTGATTTCGAAAAATCGTTGATCAGCGTCACGGCTTCTCTATAGCTCCTCCGATCGGCTGGC AAATTAGGGTTTCGTGCGAGCTTAATTCGATCGTTGGACCTGATATGCGAGCGAGTGTCCGATCAG AATCTCTCTGATCGAGAGAGAGCAAATCCCACACTCGGGGTTTCTTCCTTTGTATGGAAACTCGGA GCCGCAAGCGGGCGGAGGCGACCTCAACTGCCCCATCTTCTTCTTCTTCTTCACCTCCTCCTCCCT CAGGCCCCACCACCCGCAGCAAACGCGCTCGTCTTTCCTCTTCCTCTTCCTCCTCTGTTGCCGCTA CCACCACTGCACCTTCCTCTTCCACCCGCTCTCGCTCTTCTCGCTCCGCCGCCGCCGCTGCTACTC CCATGGACACCTCCACCGAGTCTTCTGGATTTCGCCGAGGCGGACGAGGTAACAGGGGTAACGACA ACAGTAATTCTGACAAGGGCAAGGAGAAGGAGCACGACGTTAGGATTAGGGATAGAGAAAGAGAAA GAGACCGAGCCAGAGAACAACTCAACATGGATGCTGCCGCTGCTGCTGCCGCCGCTGACGACGACG ACGACAATGACAGCGAGGATGGCAACGGGGGTTTCATGCATCCCAACATGAGCTCCGCCAGCAGTG CTTTACAAGGTTTGCTGAGGAAGCTTGGAGCTGGCCTCGATGACTTGCTTCCTTCCTCTGGTATCG GCTCTGCTTCTTCTTCCCACTTAAATGGGAGGATGAAGAAGATACTCTCTGGCTTGCGCTCCGAAG GAGAAGAGGGAAAGCAGGTCGAGGCTTTAACCCAGCTATGCGAGATGCTATCCATTGGGACTGAAG ACTCATTAAGCACCTTCTCTGTTGATTCCTTCGTCCCTGTTCTCGTTGGTCTACTAAACCATGAAA GCAATCCCGACATAATGCTTCTTGCGGCCAGGGCTCTTACCCATCTATGTGATGTTTTGCCGTCTT CTTGTGCCGCCGTTGTACATTACGGCGCTGTTTCATGCTTTGTCGCCAGATTGCTAACCATCGAGT ACATGGACTTGGCCGAGCAGGTTGGCTATCCTATCAAAACTTTGAGATTTTGTTGATTTTTATTTA ATTCTAAATTCTGGTGTTTTAATTTGCTCAAGGATCAAGCTTGAAATTTGATTTGTATGCTTAATG TGTTTTACAACAGTCTCTGCAAGCTCTCAAAAAGATATCTCAGAAGCACCCAACTGCCTGTTTGCG AGCTGGCGCTCTGATGGCAGTGCTCTCATATCTTGATTTCTTCTCCACTGGAGTCCAGGTGTGTTA TGTTTGTAACTTTTTCATTTATGCTTTACTTACACCGTTCACGTGTATCTAATGCATCTGTTGCTT CGCTTCTTGTAGCGTGTAGCACTATCTACTGCTGCAAATATGTGCAAGAAATTACCTTCTGATGCA TCTGATTATGTTATGGAAGCTGTACCATTACTGACAAACCTACTTCAGTATCATGATTCGAAGGTA ACTATCCTTCTTTTTGTTGTGATATGGTACTATCTTGTTCCGCTCTTGCCTCAGTTTCCTCCATAA TTTTTTTCGTATGGTGGCAGGTTTTGGAATATGCTTCTATATGTCTGACTCGGATTGCTGAAGCAT TTGCTTCGCACCCTGAGAAATTGGATGAATTATGCAACCATGGCCTTGTGACTCAAGCTGCGTCTC TTATTTCCACTACCAACTCAGGAGGTGGGCAAGCATCTCTGGGCGTTTCAACATACACGGTATGTG TAAATTCTTTTGTGCTTTGTGTATTTATCTTTATTCACAAGATGACATTTTCACCATATTGTGCGC AGGGGTTAATCCGATTACTTTCCACCTGTGCGAGCGGCTCACCTCTTGGATTCAGGACATTGCTTC TTCTCGGCATTAGTAGCATTCTTAAGGATATTCTGTCGGGTCCTGGTGTCTCTGCTAATGCATCTG TATCTCCAGCACTGAGCAGGCCCGCAGATCAGGTATTGATTTTACTTGTTGATATCATAATTGTTT TTTGTTCACTACCTGATTAAAAAATACTAGCTTTTGTATGAATGGTATACAGTGAAATGGTTAGGG ATCGATCACTTGGAGCTGGCATAGATTCTCTATTTCAGCTTTAGTTTCCTCATTTTTATTTGTTTG CCGTATTTTGCGTAGGTGTTTGTATAGCGGATAGATCATGATCTATGTTAAAAAGGTCTTTTGAGC TTTATTTATTTTGGAGGTCTTGTGTTAGAGGTGGTATAGAGAGTTAGAGGTTCATCAATGTGCTTT ATCTGGTTTATTTGTTGTGTAGCCCTTGGTTCGCTAATTCTTGAATTTATTCTTGTTTTCAGAAAT GTTTTGAATTATTACTTTTGTTTTGTATCTTAGATCTTTGAGATAGTCAACCTAGCGAATGAGCTC CTCCCTCCATTGCCAGAAGGAGTTATCTCCCTTCCTACTAGCACAAACACTTTGGTGAAAGGTTCA GGCCAAAAGAAATCTTCTCCAAGTACTTCAGGAAAACAAGACGATTCACCAAAAGTTTCACCTAGA GAAAAATTACTTGCTGATCAACCTGAACTTCTGCAGAAATTTGGACTGGATCTTCTTCCAGTTTTA GTGCAGGTAATTTTTTGCTGCATTTGCTACAAACAATGTTGATACCACCTGCTGTACGTCTAACTA TCCTTGCTTTCTTTCCAACAGATTTATGGTTCTAGTGTCAATGGTACTATTCGCCATAAATGTCTG TCAGTTATCGGAAAGTTGATGTATTTCAGCACTTCAGAAATGATTCAATCTCTAATTGGTGACACA AATATATCGAGGTATGCTGTTTATGATTAAAATTATGTTTTTCATGGCAGTGGGTGCAATGCAACT TCTTACGTTTATTTTTTTGCTATGTAGCTTCTTGGCTGGTGTATTGGCATGGAAAGATCCACAAGT CTTGGTTCCTGCTCTACAAGTTGCAGAAATTCTGATGGAAAAGCTTCCTGAAACATTCTCGAAAGT GTTTGTGAGGGAAGGGGTGGTTCATGCTGTAGATCAACTTGTCTTGGTTGGTAAACCTATTTCTCA TGCTTCACCTACTGATAAGGAAAATGACTGTGTGCCAGGATCTGCACGATCTAGGCGATATAGACG GCGAAGTAGTAATGCCAGTTCTGATGGAAATCAGTCAGAAGAGCTTAAGAATTCTGCGTCGGTTAA CGTAGGTGCAAACCATAATTCCCTAGATTCTCCTACCTCTAGCTTCTTGCTAAAGGAAACAGTTAG CTCCTGTGCCAAAGCATTCAAAGACAAGTACTTCCCGTCTGATGGCGGGGATCTCGATGTTGGAGT TACAGATGATCTCTTACATCTAAAGAATCTTTGCACGAAGCTAACTGCTAGTATAGATGAGCATAA AGTGAAAGGAAAGGGAAAATCCAAAGCCTCTGGGCCATGCCTTGGCGATTTTTCTGCTAGCAAGGA AGAATACTTGATTGGTGTCATTTCTGAGATACTTGGCGAGCTTAGCAAAGGGGATGGAGTCTCAAC TTTTGAGTTTATTGGCAGTGGTGTGGTTGCAGCTTTGCTTGACTATTTCTCTTGTGGGTACTTTTC CAAAGAGAAGATCTCCGAGGTTAATTTGCCCAAACTTCGCCAAGATGGGCTCAGAAGATTCAAAGC

[1478] TTTTCTAGAAGTCGCACTTCCGTCTGATGGTAATGAGGGAAAGATCCCTCCTATGACAGTTTTGAT

[1479] TCAGAAACTTCAAGATGCTTTATCTTCACTGGAACGCTTTCCCGTCGTCCTTAGTCATCCCTCAAG

[1480] GTCATTAGGTGGAAGTGCTCGTCTCTCATCTGGATTGAGTGCTTTGGCACATCCTTTAAAGTTGCG

[1481] ATTATGCCGTGCACCTGGAGAGAAGACACTACGTGATTACTCCTCCAATATTGTTCTTATAGATCC

[1482] ACTGGCAAGCATTGCAGCGGTAGAAGAATTTCTTTGGCCCCGAGTTCAACGGAGTGAATCTGGTAT

[1483] GAAGCATGCAGCGCCTGCTGGAAATACAGAGCCAGGCACGTTACCTAGCGGTGCTGGTGTTTCATC

[1484] ACCATCCTCGTCAACTCCGGCTTCCACCACACGTCGTCATTCTTCTAGATCTAGATCAGCAATTAA

[1485] CATAGGTGATACCTCAAAGAAAGAAGCTGTGCATGAGAAAGGTACCAGCTCATCTAAAGGAAAAGG

[1486] TAAAGGCGTTATGAAGCCCGTTCCAGCTGATAAGGGGCCTCAAACAAGGAGCAGTGCTCAAAGAAG

[1487] AGCTGTCCTTGACAAAGATACACATATGAAACCAGCTAGCGGAGACTCCAGCTCCGAGGTATGTTA

[1488] TTGTACAAAGTTTTGGATTACATGGTTGTTTAATTGTGTTGCATCATATGATATTTGTGGCGTGAT

[1489] CTGCTAATGCACCACTATCTTACCTTTATGCCTTGCAGGATGAGGAATTGGAAATAGCCCCTGTCG

[1490] ACATTGATGATGCCTTGGTGATTGAAGAGGATGACATGTCTGATGATGAAGATGATGATAATGACG

[1491] ATGTAAGTATTCCTTCATATTTGTTTTTTAACCAGTATGTACATTACACTCCGTAGTTATTTCTCT

[1492] TGCTAACAACATGAAAGATGCTACATTGCCAATAACGCTTGATAGCTTCCAAAATTTTGGATTAGT

[1493] TTGCCATGCTGAGACGAACTCATATTGAACATTTCCTTTTAGAATTGACAAAAATAATTACTAACT

[1494] TCATACCACTTCTCTTATTTATCTGGCTGCTATCGGTCTTAATTTCTACAATAACATTACAATCAT

[1495] ATCTATACATTCGTTTCCAGGTGTTGGATGACAGTCTTCCCATGTGCACGCCTGATAAAGTGCATG

[1496] ATGTAAAATTGGGGGACGCAGTGGATGATGACGGTGGTGCTGGTCTGGCCTCTAGCGGGCGACAGA

[1497] TGAATCCAGCTTCAGGTGGCACTAGTGGAGCAGCAGCAGCGAGGGGATCTGATTCTATTGATGCTG

[1498] GCATTGGGAATTCTTACGGTTCTAGGGGTGCACTCTCCTTTGCTGCTGCGGCAATGGCTGGGCTTG

[1499] GAGCTGCCAGTGGTAGAGGTATCAGGGGAAGTAGAGACCTGCATGGGCGTACCCTATATCGAAGTT

[1500] CCGACGAGCCCTCTAAGTTATTATTTACTGCAGGAGGAAAGCAACTTAGTAGGCATTTGACGATAT

[1501] ATCAGGCTGTGCAGCGACAACTTATGCTAGATGAAGACGATGATGAAAGGTTCGGTGGCAGCGATT

[1502] TCATGTCAAGTGATGGAAGCAGATTCAATGATATTTACACCATCATGTACCAGAGGCCGGAGAGTC

[1503] AAGCGAATAGGTTGTCTGTTGGTGCAGCAAGTTCTACCACACTATCAAAATCCACTAAATCTGCTA

[1504] CTGCCAATTCTAGCGTAGAATCTCAGTCATATAAGGCATCTCTTTTGGATAGTATCTTACAAGGGG

[1505] AGCTTCCGTGCGATCTTGAGAAGTCAAATTCTACTTATAATGTTCTGGCGCTGTTGCGTGTATTAG

[1506] AGGGGTTAAATCAGCTTGGCCCTCGGTTGAGAGCCCAAACTGTTTCTGATCGTTTCGCAGAGGGTA

[1507] AAATTACAAGTCTAGATGATCTGAAGACAACTGCTGCTGCTAAGATTTCTCATGAAGAATTCATCA

[1508] ATAGCAAACTTACACCCAAATTAGCTCGACAGATCCAGGATGCGCTTGCTTTGTGCAGTGGTAGTC

[1509] TTCCCTCTTGGTGCTACCAGTTGACTAGAGCATGCCCGTTTTTGTTTCCGTTTCAGACCCGGAGAC

[1510] AGTATTTCTATTCAACTGCTTTTGGGTTGTCCCGCGCATTGAACCGCTTGCAGCAGCAGCAAGGTG

[1511] CTGACGGCAGTGGGTCTACAAATGAACGAGAGATGAGAATAGGGAGGTTGCAGCGCCAGAAAGTCC

[1512] GTGTATCCCGAAATAGAATATTAGATTCTGCTGCGAAAGTTATGGAGATGTATTCTAGCCAAAAAG

[1513] CTGTGCTTGAAGTAGAATATTTTGGTGAAGTTGGTACTGGTCTAGGCCCTACACTTGAGTTTTACA

[1514] CACTCCTAAGCCATGATCTACAAAAGGTTGCGCTTGGGATGTGGAGATCAAATTCTGGTGACATGG

[1515] TATCTATGCAAATTGATAGAGGTGAGATTGAAGACGGGAAATCATCCGCAGCTAGGGACAGAGATA

[1516] TAGTTCAGGCACCGCTTGGGTTGTTTCCTCGACCTTGGCCCTCTACAGCTGACGTATCTGAAGGTA

[1517] GTCGGTTTCATAAAGTCATTGAATATTTCCGGCTTTTAGGGCGTGTGATGGCAAAAGCACTTCAAG

[1518] ATGGACGGCTACTGGACGTCCCATTGAGTACAGCGTTTTATAAGCTTATTCTTGGTCAAGTGAGTT

[1519] TTTTTAACTTGGTTTGTTTAGTTGAGATTGAAATAGTTGGTTTTCATTGTTGTTTTATTTATTTAT

[1520] TTCCCCCTCTTATTATTTTTCTCGCTTAAGCAGGAGCTTGATTTGCATGATATCATATTATTTGAT

[1521] ACCGAGCTTGGCAAGACTTTGCAAGAGTTTCGTGTTCTTGTTGGCCGCAAGCACTATCTGGAGGCA

[1522] GTAGGCGGTGACAATTGCAGCGCTGGTTCTGATTTATGTTTACGTGGATCCCGTATAGAAGATCTC

[1523] TGCTTGGACTTCACGCTTCCTGGCTATCCTGAATACATTCTGAGACCAGGAGATGAAATTGTACCG

[1524] TCTTATAGCCTACAACCCCACGTCTTACTTTTCTTTTAGTCCCTGTCCATGATTGCTGATGCCGTG

[1525] CTCTTCTGTTACAGGTTGATATGAATAGTCTTGAAGAGTATATATCCCTTGTCGTTGATGCTACTG

[1526] TCAAGAGAGGAGTTACCCGGCAGATTGAAGCCTTTAGATCTGGATTCAATCAGGTTAGCAGTTTCG

[1527] CAGACTCTGCTGTCTCTCTCTCTCCCTCTCTCTCTCTTCATGTTGCCTGTGGCTTCTAAATAATAC CGAAAGTGTGGTTTCTGTTGATTAGAGACTTCTTCTGTTTTTGTTTTAGGCTGAGCTCAAATTTTT GCCTGCATTTTTTATCTTTACAAAACGTCTTTGTTAATTCACATAATTCTCAGGTGTTTGACGTAA CATCTCTACAAATATTCACCCCTTCCGAGCTGGACTATTTGCTGTGTGGTCGTAGAGAGTTGTGGG AGGTGATTTTTCTACCTTTTTAGTTTTCCACACCCAGGAGACTTGAATCCCTTACATAGAATTTCA TTTCTGAAACCTTTTATTTTTGCAGGCGGAGACTCTTGCTGAACATATCAAGTTTGATCACGGTTA TACTGCTAAAAGTCCGGCAATCATTAATGTATGTCAATTTATCCGTTTATATTGAGCAATTAGAAA CCATTTCGGTTTACAATCAGCTTTTGCTACTGCACTGGCGATTTTTTTCTGAGTCTTATTTTCACC CTCACTGTAGTTACTGGAGATCATGGGAGAACTTACAGCGGATCAGCAGAGGGCTTTCTGCCAATT TGTAACTGGAGCTCCTAGGCTTCCTCCTGGTGGGCTAGCTGTTTTGAATCCAAAGCTGACGATTGT GAGAAAGGTAAAAAATCTTTACTTAATATCTATCTATGCGTGGTCTATGGGCTTTTGGTCTTATTT TCCAAGATCAGACACGCATTGTGGTGTTTTATCAGATATAATATTGGCTCTTTGATTTCGTGAAGC TTTTCTGTAATGGTTCTCAATGGGCTTCTAAAATATTGTTGTCTGCAGCATTCATCAACCTCAAGT GCGGCAGCGAACGGAACAGGGGCTTCGGAGACGGCAGACGATGATCTTCCCAGCGTAATGACTTGC GCCAACTATCTTAAGCTCCCTCCTTATTCGACAAAGGTGAGTCATGTCACTTGTTGTCTTTGAGTC CATCATGTGTTCCGTGAAGTTGTTGTTTGTAAGCCTGAGGACTTGTTGTACCTGAAACAGGAAATC ATGTACAAGAAACTGCTCTACGCCATCAACGAAGGGCAAGGATCGTTCGACCTGTCATAAGCAGAG GCTCCAACTCTCGCTCTCTCCTCTCTCTCTCTCTCTTTGTACATTTACATCGGAAGACGCTGATTT TGATTTTGCTTTTCGGGTTTGAATGATCTGACAAAAGCCGAAGATGCCCCGGGAGACTTGTAATGA GGATATAGAGAATGTGTTGTCTGAAACCTTTGTGGATGTTTTGGTGCATTCCTCTTTGTCTCTTGC AGAAAGAAAAGCTATTTATTGTGTTGTAGATAAAGAAAGATGATAGGCTTATCTTCCCCTTTTAGA TTTCATTTGGTCAGTCAAAAACCATGGTAACTTTAAGTTGGATTACCAGCAAAAACTAGAATATGG ACGGCACTTTTATTAAACCATTGAGTTATAAAGGTTAGCTTCAATATTTTTCCTTGTGCCTCCTCC CTCGTATGATTTCTCTCCCA

[1528] UPL3-8 protein sequence ( 922G>A single base substitution resulting in a Glu308Lys substitution) ( 2019-M2-102 Tarvense 15299 ) ( SEQ ID NO : 64 ) :

[1529] METRSRKRAEATSTAPSSSSSSPPPPSGPTTRSKRARLSSSSSSSVAATTTAPSSSTRSRSSRSAA

[1530] AAATPMDTSTESSGFRRGGRGNRGNDNSNSDKGKEKEHDVRIRDRERERDRAREQLNMDAAAAAAA

[1531] ADDDDDNDSEDGNGGFMHPNMSSASSALQGLLRKLGAGLDDLLPSSGIGSASSSHLNGRMKKILSG LRSEGEEGKQVEALTQLCEMLSIGTEDSLSTFSVDSFVPVLVGLLNHESNPDIMLLAARALTHLCD VLPSSCAAVVHYGAVSCFVARLLTIEYMDLAEQSLQALKKISQKHPTACLRAGALMAVLSYLDFFS TGVQRVALSTAANMCKKLPSDASDYVMEAVPLLTNLLQYHDSKVLEYASICLTRIAEAFASHPEKL DELCNHGLVTQAASLISTTNSGGGQASLGVSTYTGLIRLLSTCASGSPLGFRTLLLLGISSILKDI LSGPGVSANASVSPALSRPADQIFEIVNLANELLPPLPEGVISLPTSTNTLVKGSGQKKSSPSTSG KQDDSPKVSPREKLLADQPELLQKFGLDLLPVLVQIYGSSVNGTIRHKCLSVIGKLMYFSTSEMIQ SLIGDTNISSFLAGVLAWKDPQVLVPALQVAEILMEKLPETFSKVFVREGVVHAVDQLVLVGKPIS HASPTDKENDCVPGSARSRRYRRRSSNASSDGNQSEELKNSASVNVGANHNSLDSPTSSFLLKETV SSCAKAFKDKYFPSDGGDLDVGVTDDLLHLKNLCTKLTASIDEHKVKGKGKSKASGPCLGDFSASK EEYLIGVISEILGELSKGDGVSTFEFIGSGVVAALLDYFSCGYFSKEKISEVNLPKLRQDGLRRFK AFLEVALPSDGNEGKIPPMTVLIQKLQDALSSLERFPVVLSHPSRSLGGSARLSSGLSALAHPLKL RLCRAPGEKTLRDYSSNIVLIDPLASIAAVEEFLWPRVQRSESGMKHAAPAGNTEPGTLPSGAGVS SPSSSTPASTTRRHSSRSRSAINIGDTSKKEAVHEKGTSSSKGKGKGVMKPVPADKGPQTRSSAQR RAVLDKDTHMKPASGDSSSEVLDDSLPMCTPDKVHDVKLGDAVDDDGGAGLASSGRQMNPASGGTS GAAAARGSDSIDAGIGNSYGSRGALSFAAAAMAGLGAASGRGIRGSRDLHGRTLYRSSDEPSKLLF TAGGKQLSRHLTIYQAVQRQLMLDEDDDERFGGSDFMSSDGSRFNDIYTIMYQRPESQANRLSVGA ASSTTLSKSTKSATANSSVESQSYKASLLDSILQGELPCDLEKSNSTYNVLALLRVLEGLNQLGPR LRAQTVSDRFAEGKITSLDDLKTTAAAKISHEEFINSKLTPKLARQIQDALALCSGSLPSWCYQLT RACPFLFPFQTRRQYFYSTAFGLSRALNRLQQQQGADGSGSTNEREMRIGRLQRQKVRVSRNRILD SAAKVMEMYSSQKAVLEVEYFGEVGTGLGPTLEFYTLLSHDLQKVALGMWRSNSGDMVSMQIDRGE IEDGKSSAARDRDIVQAPLGLFPRPWPSTADVSEGSRFHKVIEYFRLLGRVMAKALQDGRLLDVPL STAFYKLILGQELDLHDI ILFDTELGKTLQEFRVLVGRKHYLEAVGGDNCSAGSDLCLRGSRIEDL

[1532] CLDFTLPGYPEYILRPGDEIVDMNSLEEYISLVVDATVKRGVTRQIEAFRSGFNQVFDVTSLQIFT PSELDYLLCGRRELWEAETLAEHIKFDHGYTAKSPAIINLLEIMGELTADQQRAFCQFVTGAPRLP PGGLAVLNPKLTIVRKHSSTSSAAANGTGASETADDDLPSVMTCANYLKLPPYSTKEIMYKKLLYA

[1533] INEGQGSFDLS upl3-4 nucleic acid sequence ( 4699G>A single base substitution resulting in a Glyl567Arg substitution ) ( 2020-M2-437 Tarvense 15299 ) ( SEQ ID NO : 65 ) :

[1534] CGATATCGTATGGAGCTTTAATGGAGCAAACGAATCACACGTAGCGGATGGATCCCCGTGTCCAGT TCTTGTCCCAAAAGCCCGAAGGAGCAGGGGAAACGGTATCGTATTCGCTTCATTCTATATTCTGTA CAAAAGCCCTTTTCTCCTCCCTTTTGTTTCATTCCCCCACTCGATTTAGGGTTTTCTTCTAATCCG GTGTGTAAAATTGAAGATCCTTCTTCTCTTCCTTGCTTCCCCCTCTCGATTCCGATCTCATCATCC CTTTTTGATTTCGAAAAATCGTTGATCAGCGTCACGGCTTCTCTATAGCTCCTCCGATCGGCTGGC AAATTAGGGTTTCGTGCGAGCTTAATTCGATCGTTGGACCTGATATGCGAGCGAGTGTCCGATCAG AATCTCTCTGATCGAGAGAGAGCAAATCCCACACTCGGGGTTTCTTCCTTTGTATGGAAACTCGGA GCCGCAAGCGGGCGGAGGCGACCTCAACTGCCCCATCTTCTTCTTCTTCTTCACCTCCTCCTCCCT CAGGCCCCACCACCCGCAGCAAACGCGCTCGTCTTTCCTCTTCCTCTTCCTCCTCTGTTGCCGCTA CCACCACTGCACCTTCCTCTTCCACCCGCTCTCGCTCTTCTCGCTCCGCCGCCGCCGCTGCTACTC CCATGGACACCTCCACCGAGTCTTCTGGATTTCGCCGAGGCGGACGAGGTAACAGGGGTAACGACA ACAGTAATTCTGACAAGGGCAAGGAGAAGGAGCACGACGTTAGGATTAGGGATAGAGAAAGAGAAA GAGACCGAGCCAGAGAACAACTCAACATGGATGCTGCCGCTGCTGCTGCCGCCGCTGACGACGACG ACGACAATGACAGCGAGGATGGCAACGGGGGTTTCATGCATCCCAACATGAGCTCCGCCAGCAGTG CTTTACAAGGTTTGCTGAGGAAGCTTGGAGCTGGCCTCGATGACTTGCTTCCTTCCTCTGGTATCG GCTCTGCTTCTTCTTCCCACTTAAATGGGAGGATGAAGAAGATACTCTCTGGCTTGCGCTCCGAAG GAGAAGAGGGAAAGCAGGTCGAGGCTTTAACCCAGCTATGCGAGATGCTATCCATTGGGACTGAAG ACTCATTAAGCACCTTCTCTGTTGATTCCTTCGTCCCTGTTCTCGTTGGTCTACTAAACCATGAAA GCAATCCCGACATAATGCTTCTTGCGGCCAGGGCTCTTACCCATCTATGTGATGTTTTGCCGTCTT CTTGTGCCGCCGTTGTACATTACGGCGCTGTTTCATGCTTTGTCGCCAGATTGCTAACCATCGAGT ACATGGACTTGGCCGAGCAGGTTGGCTATCCTATCAAAACTTTGAGATTTTGTTGATTTTTATTTA ATTCTAAATTCTGGTGTTTTAATTTGCTCAAGGATCAAGCTTGAAATTTGATTTGTATGCTTAATG TGTTTTACAACAGTCTCTGCAAGCTCTCAAAAAGATATCTCAGGAGCACCCAACTGCCTGTTTGCG AGCTGGCGCTCTGATGGCAGTGCTCTCATATCTTGATTTCTTCTCCACTGGAGTCCAGGTGTGTTA TGTTTGTAACTTTTTCATTTATGCTTTACTTACACCGTTCACGTGTATCTAATGCATCTGTTGCTT CGCTTCTTGTAGCGTGTAGCACTATCTACTGCTGCAAATATGTGCAAGAAATTACCTTCTGATGCA TCTGATTATGTTATGGAAGCTGTACCATTACTGACAAACCTACTTCAGTATCATGATTCGAAGGTA ACTATCCTTCTTTTTGTTGTGATATGGTACTATCTTGTTCCGCTCTTGCCTCAGTTTCCTCCATAA TTTTTTTCGTATGGTGGCAGGTTTTGGAATATGCTTCTATATGTCTGACTCGGATTGCTGAAGCATTTGCTTCGCACCCTGAGAAATTGGATGAATTATGCAACCATGGCCTTGTGACTCAAGCTGCGTCTC TTATTTCCACTACCAACTCAGGAGGTGGGCAAGCATCTCTGGGCGTTTCAACATACACGGTATGTG TAAATTCTTTTGTGCTTTGTGTATTTATCTTTATTCACAAGATGACATTTTCACCATATTGTGCGC AGGGGTTAATCCGATTACTTTCCACCTGTGCGAGCGGCTCACCTCTTGGATTCAGGACATTGCTTC TTCTCGGCATTAGTAGCATTCTTAAGGATATTCTGTCGGGTCCTGGTGTCTCTGCTAATGCATCTG TATCTCCAGCACTGAGCAGGCCCGCAGATCAGGTATTGATTTTACTTGTTGATATCATAATTGTTT TTTGTTCACTACCTGATTAAAAAATACTAGCTTTTGTATGAATGGTATACAGTGAAATGGTTAGGG ATCGATCACTTGGAGCTGGCATAGATTCTCTATTTCAGCTTTAGTTTCCTCATTTTTATTTGTTTG CCGTATTTTGCGTAGGTGTTTGTATAGCGGATAGATCATGATCTATGTTAAAAAGGTCTTTTGAGC TTTATTTATTTTGGAGGTCTTGTGTTAGAGGTGGTATAGAGAGTTAGAGGTTCATCAATGTGCTTT ATCTGGTTTATTTGTTGTGTAGCCCTTGGTTCGCTAATTCTTGAATTTATTCTTGTTTTCAGAAAT GTTTTGAATTATTACTTTTGTTTTGTATCTTAGATCTTTGAGATAGTCAACCTAGCGAATGAGCTC CTCCCTCCATTGCCAGAAGGAGTTATCTCCCTTCCTACTAGCACAAACACTTTGGTGAAAGGTTCA GGCCAAAAGAAATCTTCTCCAAGTACTTCAGGAAAACAAGACGATTCACCAAAAGTTTCACCTAGA

[1535] GAAAAATTACTTGCTGATCAACCTGAACTTCTGCAGAAATTTGGACTGGATCTTCTTCCAGTTTTA

[1536] GTGCAGGTAATTTTTTGCTGCATTTGCTACAAACAATGTTGATACCACCTGCTGTACGTCTAACTA

[1537] TCCTTGCTTTCTTTCCAACAGATTTATGGTTCTAGTGTCAATGGTACTATTCGCCATAAATGTCTG

[1538] TCAGTTATCGGAAAGTTGATGTATTTCAGCACTTCAGAAATGATTCAATCTCTAATTGGTGACACA

[1539] AATATATCGAGGTATGCTGTTTATGATTAAAATTATGTTTTTCATGGCAGTGGGTGCAATGCAACT

[1540] TCTTACGTTTATTTTTTTGCTATGTAGCTTCTTGGCTGGTGTATTGGCATGGAAAGATCCACAAGT

[1541] CTTGGTTCCTGCTCTACAAGTTGCAGAAATTCTGATGGAAAAGCTTCCTGAAACATTCTCGAAAGT

[1542] GTTTGTGAGGGAAGGGGTGGTTCATGCTGTAGATCAACTTGTCTTGGTTGGTAAACCTATTTCTCA

[1543] TGCTTCACCTACTGATAAGGAAAATGACTGTGTGCCAGGATCTGCACGATCTAGGCGATATAGACG

[1544] GCGAAGTAGTAATGCCAGTTCTGATGGAAATCAGTCAGAAGAGCTTAAGAATTCTGCGTCGGTTAA

[1545] CGTAGGTGCAAACCATAATTCCCTAGATTCTCCTACCTCTAGCTTCTTGCTAAAGGAAACAGTTAG

[1546] CTCCTGTGCCAAAGCATTCAAAGACAAGTACTTCCCGTCTGATGGCGGGGATCTCGATGTTGGAGT

[1547] TACAGATGATCTCTTACATCTAAAGAATCTTTGCACGAAGCTAACTGCTAGTATAGATGAGCATAA

[1548] AGTGAAAGGAAAGGGAAAATCCAAAGCCTCTGGGCCATGCCTTGGCGATTTTTCTGCTAGCAAGGA

[1549] AGAATACTTGATTGGTGTCATTTCTGAGATACTTGGCGAGCTTAGCAAAGGGGATGGAGTCTCAAC

[1550] TTTTGAGTTTATTGGCAGTGGTGTGGTTGCAGCTTTGCTTGACTATTTCTCTTGTGGGTACTTTTC

[1551] CAAAGAGAAGATCTCCGAGGTTAATTTGCCCAAACTTCGCCAAGATGGGCTCAGAAGATTCAAAGC

[1552] TTTTCTAGAAGTCGCACTTCCGTCTGATGGTAATGAGGGAAAGATCCCTCCTATGACAGTTTTGAT

[1553] TCAGAAACTTCAAGATGCTTTATCTTCACTGGAACGCTTTCCCGTCGTCCTTAGTCATCCCTCAAG

[1554] GTCATTAGGTGGAAGTGCTCGTCTCTCATCTGGATTGAGTGCTTTGGCACATCCTTTAAAGTTGCG

[1555] ATTATGCCGTGCACCTGGAGAGAAGACACTACGTGATTACTCCTCCAATATTGTTCTTATAGATCC

[1556] ACTGGCAAGCATTGCAGCGGTAGAAGAATTTCTTTGGCCCCGAGTTCAACGGAGTGAATCTGGTAT

[1557] GAAGCATGCAGCGCCTGCTGGAAATACAGAGCCAGGCACGTTACCTAGCGGTGCTGGTGTTTCATC

[1558] ACCATCCTCGTCAACTCCGGCTTCCACCACACGTCGTCATTCTTCTAGATCTAGATCAGCAATTAA

[1559] CATAGGTGATACCTCAAAGAAAGAAGCTGTGCATGAGAAAGGTACCAGCTCATCTAAAGGAAAAGG

[1560] TAAAGGCGTTATGAAGCCCGTTCCAGCTGATAAGGGGCCTCAAACAAGGAGCAGTGCTCAAAGAAG

[1561] AGCTGTCCTTGACAAAGATACACATATGAAACCAGCTAGCGGAGACTCCAGCTCCGAGGTATGTTA

[1562] TTGTACAAAGTTTTGGATTACATGGTTGTTTAATTGTGTTGCATCATATGATATTTGTGGCGTGAT

[1563] CTGCTAATGCACCACTATCTTACCTTTATGCCTTGCAGGATGAGGAATTGGAAATAGCCCCTGTCG

[1564] ACATTGATGATGCCTTGGTGATTGAAGAGGATGACATGTCTGATGATGAAGATGATGATAATGACG

[1565] ATGTAAGTATTCCTTCATATTTGTTTTTTAACCAGTATGTACATTACACTCCGTAGTTATTTCTCT

[1566] TGCTAACAACATGAAAGATGCTACATTGCCAATAACGCTTGATAGCTTCCAAAATTTTGGATTAGT

[1567] TTGCCATGCTGAGACGAACTCATATTGAACATTTCCTTTTAGAATTGACAAAAATAATTACTAACT

[1568] TCATACCACTTCTCTTATTTATCTGGCTGCTATCGGTCTTAATTTCTACAATAACATTACAATCAT

[1569] ATCTATACATTCGTTTCCAGGTGTTGGATGACAGTCTTCCCATGTGCACGCCTGATAAAGTGCATG

[1570] ATGTAAAATTGGGGGACGCAGTGGATGATGACGGTGGTGCTGGTCTGGCCTCTAGCGGGCGACAGA

[1571] TGAATCCAGCTTCAGGTGGCACTAGTGGAGCAGCAGCAGCGAGGGGATCTGATTCTATTGATGCTG

[1572] GCATTGGGAATTCTTACGGTTCTAGGGGTGCACTCTCCTTTGCTGCTGCGGCAATGGCTGGGCTTG

[1573] GAGCTGCCAGTGGTAGAGGTATCAGGGGAAGTAGAGACCTGCATGGGCGTACCCTATATCGAAGTT

[1574] CCGACGAGCCCTCTAAGTTATTATTTACTGCAGGAGGAAAGCAACTTAGTAGGCATTTGACGATAT

[1575] ATCAGGCTGTGCAGCGACAACTTATGCTAGATGAAGACGATGATGAAAGGTTCGGTGGCAGCGATT

[1576] TCATGTCAAGTGATGGAAGCAGATTCAATGATATTTACACCATCATGTACCAGAGGCCGGAGAGTC

[1577] AAGCGAATAGGTTGTCTGTTGGTGCAGCAAGTTCTACCACACTATCAAAATCCACTAAATCTGCTA

[1578] CTGCCAATTCTAGCGTAGAATCTCAGTCATATAAGGCATCTCTTTTGGATAGTATCTTACAAGGGG

[1579] AGCTTCCGTGCGATCTTGAGAAGTCAAATTCTACTTATAATGTTCTGGCGCTGTTGCGTGTATTAG

[1580] AGGGGTTAAATCAGCTTGGCCCTCGGTTGAGAGCCCAAACTGTTTCTGATCGTTTCGCAGAGGGTA

[1581] AAATTACAAGTCTAGATGATCTGAAGACAACTGCTGCTGCTAAGATTTCTCATGAAGAATTCATCA

[1582] ATAGCAAACTTACACCCAAATTAGCTCGACAGATCCAGGATGCGCTTGCTTTGTGCAGTGGTAGTC

[1583] TTCCCTCTTGGTGCTACCAGTTGACTAGAGCATGCCCGTTTTTGTTTCCGTTTCAGACCCGGAGAC

[1584] AGTATTTCTATTCAACTGCTTTTGGGTTGTCCCGCGCATTGAACCGCTTGCAGCAGCAGCAAGGTG CTGACGGCAGTGGGTCTACAAATGAACGAGAGATGAGAATAGGGAGGTTGCAGCGCCAGAAAGTCC GTGTATCCCGAAATAGAATATTAGATTCTGCTGCGAAAGTTATGGAGATGTATTCTAGCCAAAAAG CTGTGCTTGAAGTAGAATATTTTGGTGAAGTTGGTACTGGTCTAGGCCCTACACTTGAGTTTTACA CACTCCTAAGCCATGATCTACAAAAGGTTGCGCTTGGGATGTGGAGATCAAATTCTGGTGACATGG TATCTATGCAAATTGATAGAGGTGAGATTGAAGACGGGAAATCATCCGCAGCTAGGGACAGAGATA TAGTTCAGGCACCGCTTGGGTTGTTTCCTCGACCTTGGCCCTCTACAGCTGACGTATCTGAAGGTA GTCGGTTTCATAAAGTCATTGAATATTTCCGGCTTTTAAGGCGTGTGATGGCAAAAGCACTTCAAG ATGGACGGCTACTGGACGTCCCATTGAGTACAGCGTTTTATAAGCTTATTCTTGGTCAAGTGAGTT TTTTTAACTTGGTTTGTTTAGTTGAGATTGAAATAGTTGGTTTTCATTGTTGTTTTATTTATTTAT TTCCCCCTCTTATTATTTTTCTCGCTTAAGCAGGAGCTTGATTTGCATGATATCATATTATTTGAT ACCGAGCTTGGCAAGACTTTGCAAGAGTTTCGTGTTCTTGTTGGCCGCAAGCACTATCTGGAGGCA GTAGGCGGTGACAATTGCAGCGCTGGTTCTGATTTATGTTTACGTGGATCCCGTATAGAAGATCTC TGCTTGGACTTCACGCTTCCTGGCTATCCTGAATACATTCTGAGACCAGGAGATGAAATTGTACCG TCTTATAGCCTACAACCCCACGTCTTACTTTTCTTTTAGTCCCTGTCCATGATTGCTGATGCCGTG CTCTTCTGTTACAGGTTGATATGAATAGTCTTGAAGAGTATATATCCCTTGTCGTTGATGCTACTG TCAAGAGAGGAGTTACCCGGCAGATTGAAGCCTTTAGATCTGGATTCAATCAGGTTAGCAGTTTCG CAGACTCTGCTGTCTCTCTCTCTCCCTCTCTCTCTCTTCATGTTGCCTGTGGCTTCTAAATAATAC CGAAAGTGTGGTTTCTGTTGATTAGAGACTTCTTCTGTTTTTGTTTTAGGCTGAGCTCAAATTTTT GCCTGCATTTTTTATCTTTACAAAACGTCTTTGTTAATTCACATAATTCTCAGGTGTTTGACGTAA CATCTCTACAAATATTCACCCCTTCCGAGCTGGACTATTTGCTGTGTGGTCGTAGAGAGTTGTGGG AGGTGATTTTTCTACCTTTTTAGTTTTCCACACCCAGGAGACTTGAATCCCTTACATAGAATTTCA TTTCTGAAACCTTTTATTTTTGCAGGCGGAGACTCTTGCTGAACATATCAAGTTTGATCACGGTTA TACTGCTAAAAGTCCGGCAATCATTAATGTATGTCAATTTATCCGTTTATATTGAGCAATTAGAAA CCATTTCGGTTTACAATCAGCTTTTGCTACTGCACTGGCGATTTTTTTCTGAGTCTTATTTTCACC CTCACTGTAGTTACTGGAGATCATGGGAGAACTTACAGCGGATCAGCAGAGGGCTTTCTGCCAATT TGTAACTGGAGCTCCTAGGCTTCCTCCTGGTGGGCTAGCTGTTTTGAATCCAAAGCTGACGATTGT GAGAAAGGTAAAAAATCTTTACTTAATATCTATCTATGCGTGGTCTATGGGCTTTTGGTCTTATTT TCCAAGATCAGACACGCATTGTGGTGTTTTATCAGATATAATATTGGCTCTTTGATTTCGTGAAGC TTTTCTGTAATGGTTCTCAATGGGCTTCTAAAATATTGTTGTCTGCAGCATTCATCAACCTCAAGT GCGGCAGCGAACGGAACAGGGGCTTCGGAGACGGCAGACGATGATCTTCCCAGCGTAATGACTTGC GCCAACTATCTTAAGCTCCCTCCTTATTCGACAAAGGTGAGTCATGTCACTTGTTGTCTTTGAGTC CATCATGTGTTCCGTGAAGTTGTTGTTTGTAAGCCTGAGGACTTGTTGTACCTGAAACAGGAAATC ATGTACAAGAAACTGCTCTACGCCATCAACGAAGGGCAAGGATCGTTCGACCTGTCATAAGCAGAG GCTCCAACTCTCGCTCTCTCCTCTCTCTCTCTCTCTTTGTACATTTACATCGGAAGACGCTGATTT TGATTTTGCTTTTCGGGTTTGAATGATCTGACAAAAGCCGAAGATGCCCCGGGAGACTTGTAATGA GGATATAGAGAATGTGTTGTCTGAAACCTTTGTGGATGTTTTGGTGCATTCCTCTTTGTCTCTTGC AGAAAGAAAAGCTATTTATTGTGTTGTAGATAAAGAAAGATGATAGGCTTATCTTCCCCTTTTAGA TTTCATTTGGTCAGTCAAAAACCATGGTAACTTTAAGTTGGATTACCAGCAAAAACTAGAATATGG ACGGCACTTTTATTAAACCATTGAGTTATAAAGGTTAGCTTCAATATTTTTCCTTGTGCCTCCTCC CTCGTATGATTTCTCTCCCA

[1585] UPL3-4 protein sequence ( 4699G>A single base substitution resulting in a Glyl567Arg substitution) ( 2020-M2 -437 Tarvense 15299 ) (SEQ ID NO : 66 ) :

[1586] METRSRKRAEATSTAPSSSSSSPPPPSGPTTRSKRARLSSSSSSSVAATTTAPSSSTRSRSSRSAA

[1587] AAATPMDTSTESSGFRRGGRGNRGNDNSNSDKGKEKEHDVRIRDRERERDRAREQLNMDAAAAAAA ADDDDDNDSEDGNGGFMHPNMSSASSALQGLLRKLGAGLDDLLPSSGIGSASSSHLNGRMKKILSG LRSEGEEGKQVEALTQLCEMLSIGTEDSLSTFSVDSFVPVLVGLLNHESNPDIMLLAARALTHLCD VLPSSCAAVVHYGAVSCFVARLLTIEYMDLAEQSLQALKKISQEHPTACLRAGALMAVLSYLDFFS TGVQRVALSTAANMCKKLPSDASDYVMEAVPLLTNLLQYHDSKVLEYASICLTRIAEAFASHPEKL DELCNHGLVTQAASLISTTNSGGGQASLGVSTYTGLIRLLSTCASGSPLGFRTLLLLGISSILKDI LSGPGVSANASVSPALSRPADQI FEIVNLANELLPPLPEGVISLPTSTNTLVKGSGQKKSSPSTSG

[1588] KQDDSPKVSPREKLLADQPELLQKFGLDLLPVLVQIYGSSVNGTIRHKCLSVIGKLMYFSTSEMIQ

[1589] SLIGDTNISS FLAGVLAWKDPQVLVPALQVAEILMEKLPET FSKVFVREGVVHAVDQLVLVGKPIS

[1590] HASPTDKENDCVPGSARSRRYRRRSSNASSDGNQSEELKNSASVNVGANHNSLDSPTSS FLLKETV

[1591] SSCAKAFKDKYFPSDGGDLDVGVTDDLLHLKNLCTKLTASIDEHKVKGKGKSKASGPCLGDFSASK

[1592] EEYLIGVISE ILGELSKGDGVSTFEFIGSGVVAALLDY FSCGY FSKEKI SEVNLPKLRQDGLRRFK

[1593] AFLEVALPSDGNEGKIPPMTVLIQKLQDALSSLERFPVVLSHPSRSLGGSARLSSGLSALAHPLKL

[1594] RLCRAPGEKTLRDYSSNIVLIDPLAS IAAVEEFLWPRVQRSESGMKHAAPAGNTEPGTLPSGAGVS

[1595] SPSSSTPASTTRRHSSRSRSAINIGDTSKKEAVHEKGTSSSKGKGKGVMKPVPADKGPQTRSSAQR

[1596] RAVLDKDTHMKPASGDSSSEVLDDSLPMCTPDKVHDVKLGDAVDDDGGAGLASSGRQMNPASGGTS

[1597] GAAAARGSDS IDAGIGNSYGSRGALS FAAAAMAGLGAASGRGIRGSRDLHGRTLYRSSDEPSKLLF

[1598] TAGGKQLSRHLTIYQAVQRQLMLDEDDDERFGGSDFMSSDGSRFNDIYT IMYQRPESQANRLSVGA

[1599] ASSTTLSKSTKSATANSSVESQSYKASLLDSILQGELPCDLEKSNSTYNVLALLRVLEGLNQLGPR

[1600] LRAQTVSDRFAEGKITSLDDLKTTAAAKISHEEFINSKLTPKLARQIQDALALCSGSLPSWCYQLT

[1601] RACPFLFPFQTRRQYFYSTAFGLSRALNRLQQQQGADGSGSTNEREMRIGRLQRQKVRVSRNRILD

[1602] SAAKVMEMYSSQKAVLEVEYFGEVGTGLGPTLEFYTLLSHDLQKVALGMWRSNSGDMVSMQIDRGE

[1603] IEDGKSSAARDRDIVQAPLGLFPRPWPSTADVSEGSRFHKVIEYFRLLRRVMAKALQDGRLLDVPL

[1604] STAFYKLILGQELDLHDI ILFDTELGKTLQEFRVLVGRKHYLEAVGGDNCSAGSDLCLRGSRIEDL

[1605] CLDFTLPGYPEYILRPGDEIVDMNSLEEYI SLVVDATVKRGVTRQIEAFRSGFNQVFDVTSLQI FT

[1606] PSELDYLLCGRRELWEAETLAEHIKFDHGYTAKSPAI INLLEIMGELTADQQRAFCQFVTGAPRLP

[1607] PGGLAVLNPKLTIVRKHSSTSSAAANGTGASETADDDLPSVMTCANYLKLPPYSTKE IMYKKLLYA INEGQGSFDLS upl3-9 nucleic acid sequence ( 5341C>T s ingle base substitution resulting in a Leul781 Phe substitution ) ( 2019 M2 211 Tarvense 15299 ) ( SEQ ID NO : 67 ) :

[1608] CGATATCGTATGGAGCTTTAATGGAGCAAACGAATCACACGTAGCGGATGGATCCCCGTGTCCAGT

[1609] TCTTGTCCCAAAAGCCCGAAGGAGCAGGGGAAACGGTATCGTATTCGCTTCATTCTATATTCTGTA

[1610] CAAAAGCCCTTTTCTCCTCCCTTTTGTTTCATTCCCCCACTCGATTTAGGGTTTTCTTCTAATCCG

[1611] GTGTGTAAAATTGAAGATCCTTCTTCTCTTCCTTGCTTCCCCCTCTCGATTCCGATCTCATCATCC

[1612] CTTTTTGATTTCGAAAAATCGTTGATCAGCGTCACGGCTTCTCTATAGCTCCTCCGATCGGCTGGC

[1613] AAATTAGGGTTTCGTGCGAGCTTAATTCGATCGTTGGACCTGATATGCGAGCGAGTGTCCGATCAG

[1614] AATCTCTCTGATCGAGAGAGAGCAAATCCCACACTCGGGGTTTCTTCCTTTGTATGGAAACTCGGA

[1615] GCCGCAAGCGGGCGGAGGCGACCTCAACTGCCCCATCTTCTTCTTCTTCTTCACCTCCTCCTCCCT

[1616] CAGGCCCCACCACCCGCAGCAAACGCGCTCGTCTTTCCTCTTCCTCTTCCTCCTCTGTTGCCGCTA

[1617] CCACCACTGCACCTTCCTCTTCCACCCGCTCTCGCTCTTCTCGCTCCGCCGCCGCCGCTGCTACTC

[1618] CCATGGACACCTCCACCGAGTCTTCTGGATTTCGCCGAGGCGGACGAGGTAACAGGGGTAACGACA

[1619] ACAGTAATTCTGACAAGGGCAAGGAGAAGGAGCACGACGTTAGGATTAGGGATAGAGAAAGAGAAA

[1620] GAGACCGAGCCAGAGAACAACTCAACATGGATGCTGCCGCTGCTGCTGCCGCCGCTGACGACGACG

[1621] ACGACAATGACAGCGAGGATGGCAACGGGGGTTTCATGCATCCCAACATGAGCTCCGCCAGCAGTG

[1622] CTTTACAAGGTTTGCTGAGGAAGCTTGGAGCTGGCCTCGATGACTTGCTTCCTTCCTCTGGTATCGGCTCTGCTTCTTCTTCCCACTTAAATGGGAGGATGAAGAAGATACTCTCTGGCTTGCGCTCCGAAGGAGAAGAGGGAAAGCAGGTCGAGGCTTTAACCCAGCTATGCGAGATGCTATCCATTGGGACTGAAG

[1623] ACTCATTAAGCACCTTCTCTGTTGATTCCTTCGTCCCTGTTCTCGTTGGTCTACTAAACCATGAAA

[1624] GCAATCCCGACATAATGCTTCTTGCGGCCAGGGCTCTTACCCATCTATGTGATGTTTTGCCGTCTT

[1625] CTTGTGCCGCCGTTGTACATTACGGCGCTGTTTCATGCTTTGTCGCCAGATTGCTAACCATCGAGT

[1626] ACATGGACTTGGCCGAGCAGGTTGGCTATCCTATCAAAACTTTGAGATTTTGTTGATTTTTATTTA

[1627] ATTCTAAATTCTGGTGTTTTAATTTGCTCAAGGATCAAGCTTGAAATTTGATTTGTATGCTTAATG

[1628] TGTTTTACAACAGTCTCTGCAAGCTCTCAAAAAGATATCTCAGGAGCACCCAACTGCCTGTTTGCG

[1629] AGCTGGCGCTCTGATGGCAGTGCTCTCATATCTTGATTTCTTCTCCACTGGAGTCCAGGTGTGTTA

[1630] TGTTTGTAACTTTTTCATTTATGCTTTACTTACACCCTTCACGTCTATCTAATGCATCTGTTGCTT CGCTTCTTGTAGCGTGTAGCACTATCTACTGCTGCAAATATGTGCAAGAAATTACCTTCTGATGCA TCTGATTATGTTATGGAAGCTGTACCATTACTGACAAACCTACTTCAGTATCATGATTCGAAGGTA ACTATCCTTCTTTTTGTTGTGATATGGTACTATCTTGTTCCGCTCTTGCCTCAGTTTCCTCCATAA TTTTTTTCGTATGGTGGCAGGTTTTGGAATATGCTTCTATATGTCTGACTCGGATTGCTGAAGCAT TTGCTTCGCACCCTGAGAAATTGGATGAATTATGCAACCATGGCCTTGTGACTCAAGCTGCGTCTC TTATTTCCACTACCAACTCAGGAGGTGGGCAAGCATCTCTGGGCGTTTCAACATACACGGTATGTG TAAATTCTTTTGTGCTTTGTGTATTTATCTTTATTCACAAGATGACATTTTCACCATATTGTGCGC AGGGGTTAATCCGATTACTTTCCACCTGTGCGAGCGGCTCACCTCTTGGATTCAGGACATTGCTTC TTCTCGGCATTAGTAGCATTCTTAAGGATATTCTGTCGGGTCCTGGTGTCTCTGCTAATGCATCTG TATCTCCAGCACTGAGCAGGCCCGCAGATCAGGTATTGATTTTACTTGTTGATATCATAATTGTTT TTTGTTCACTACCTGATTAAAAAATACTAGCTTTTGTATGAATGGTATACAGTGAAATGGTTAGGG ATCGATCACTTGGAGCTGGCATAGATTCTCTATTTCAGCTTTAGTTTCCTCATTTTTATTTGTTTG CCGTATTTTGCGTAGGTGTTTGTATAGCGGATAGATCATGATCTATGTTAAAAAGGTCTTTTGAGC TTTATTTATTTTGGAGGTCTTGTGTTAGAGGTGGTATAGAGAGTTAGAGGTTCATCAATGTGCTTT ATCTGGTTTATTTGTTGTGTAGCCCTTGGTTCG...

Claims

WHAT IS CLAIMED IS:

1. A mutant oilseed plant exhibiting increased plant size, increased seed size, increased seed weight, increased seed oil content, and / or increased seed protein content, the mutant oilseed plant comprising: a loss-of- function mutation in a dal nucleic acid sequence, wherein the dal nucleic acid sequence has at least 95% sequence identity to the sequence shown in SEQ ID NO: 1; a loss-of- function mutation in a darl nucleic acid sequence, wherein the darl nucleic acid sequence has at least 95% sequence identity to the sequence shown in SEQ ID NO:3; a loss-of- function mutation in a upl3 nucleic acid sequence, wherein the upl3 nucleic acid sequence has at least 95% sequence identity to the sequence shown in SEQ ID NO:5; and / or a loss-of- function mutation in a da2 nucleic acid sequence, wherein the da2 nucleic acid sequence has at least 95% sequence identity to the sequence shown in SEQ ID NO: 7, wherein the mutant oilseed plant exhibits increased plant size, increased seed size, increased seed weight, increased seed oil content, and / or increased seed protein content relative to a corresponding oilseed plant lacking the loss-of-function mutation(s).

2. The mutant oilseed plant of claim 1, wherein the mutant dal nucleic acid sequence is selected from SEQ ID NOs: 9, 11, 13, 37, 39, 41, 21, 73, 77, 81, 83, 89, 93, 97, 107, and 111.

3. The mutant oilseed plant of claim 1 or 2, wherein the mutant darl nucleic acid sequence is selected from SEQ ID NOs: 15, 17, 19, 51, 47, 49, 53, 55, 22, 75, 79, 85, 87, 91, 95, 99, and 113.

4. The mutant oilseed plant of any one of claims 1, 2 or 3, wherein the mutant upl3 nucleic acid sequence is selected from SEQ ID NOs: 25, 27, 29, 65, 57, 59, 61, 63, 67, 69, 71, 101, 103, 105, 109, and 115.

5. The mutant oilseed plant of any one of claims 1, 2, 3 or 4, wherein the mutant da2 nucleic acid sequence is selected from SEQ ID NOs: 31, 33, 35, 43, 45, 117, 119, 121, 123, 125, 127, 129, 131, 133, and 135.

6. The mutant oilseed plant of any of the preceding claims, wherein the oilseed plant exhibits at least a 5% (e.g., at least 5% to 30%) increase in height and / or organ size (e.g., leaves, stems, roots, flowers, and / or seed pods) relative to an oilseed plant lacking the loss-of-function mutation(s).

7. The mutant oilseed plant of any of the preceding claims, wherein the oilseed plant exhibits at least a 10% (e.g., at least 10% to 60%) increase in seed size relative to an oilseed plant lacking the loss-of-function mutation(s).

8. The mutant oilseed plant of any of the preceding claims, wherein the oilseed plant exhibits at least a 10% (e.g., at least 10% to 60%) increase in seed weight relative to an oilseed plant lacking the loss-of-function mutation(s).

9. The mutant oilseed plant of any of the preceding claims, wherein the oilseed plant exhibits at least a 5% (e.g., at least 5% to 70%) increase in the amount of seed oil content relative to an oilseed plant lacking the loss-of-function mutation(s).

10. The mutant oilseed plant of any of the preceding claims, wherein the oilseed plant exhibits at least a 5% (e.g., at least 5% to 95%) increase in the amount of seed protein content relative to an oilseed plant lacking the loss-of-function mutation(s).

11. The mutant oilseed plant of any of the preceding claims, wherein the oilseed plant further comprises a mutation in transparent testa8 (tt8).

12. The mutant oilseed plant of any of the preceding claims, wherein the oilseed plant further comprises a mutation in a fatty acid elongase 1 (fael) nucleic acid.

13. The mutant oilseed plant of any of the preceding claims, wherein the oilseed plant further comprises a mutation in tt8 and mfael.

14. The mutant oilseed plant of any of the preceding claims, wherein the oilseed plant is selected from pennycress, rapeseed, soybean, sunflower, canola, flax, camelina, carinata, crambe, and lepidium plants.

15. The mutant oilseed plant of any of the preceding claims, wherein the mutation is selected from a point mutation, a deletion, an insertion, a duplication, and an inversion.

16. Seed produced by the oilseed plant of any of the preceding claims, wherein the seed comprises at least one of the loss-of-function mutation(s).

17. A method of making a mutant oilseed plant exhibiting increased plant size, increased seed size, increased seed weight, increased seed oil content, and / or increased seed protein content, the method comprising: mutating cells from an oilseed plant; regenerating mutant oilseed plants from the mutated cells; and selecting mutant oilseed plants comprising: a loss-of-function mutation in a dal nucleic acid sequence, wherein the dal nucleic acid sequence has at least 95% sequence identity to the sequence shown in SEQ ID NO:1; a loss-of-function mutation in a darl nucleic acid sequence, wherein the darl nucleic acid sequence has at least 95% sequence identity to the sequence shown in SEQ ID NO:3;a loss-of-fimction mutation in a upl3 nucleic acid sequence, wherein the upl3 nucleic acid sequence has at least 95% sequence identity to the sequence shown in SEQ ID NO: 5, and / or a loss-of-fiinction mutation in a da2 nucleic acid sequence, wherein the da2 nucleic acid sequence has at least 95% sequence identity to the sequence shown in SEQ ID NO: 7; thereby making an oilseed plant that exhibits increased plant size, increased seed size, increased seed weight, increased seed oil content, and / or increased seed protein content relative to a corresponding oilseed plant lacking the loss-of-function mutation(s).

18. The method of claim 18, wherein the loss-of-function mutations are introduced using EMS, CRISPR, TALEN, Zinc-finger nuclease, or a combination thereof.

19. The method of claim 18 or 19, wherein the mutation is selected from a point mutation, a deletion, an insertion, a duplication, and an inversion.

20. A method of planting the oilseed seed of claim 16.

21. The method of claim 20, wherein the oilseed seed can be planted at a depth of up to 1.5 inches.

22. A method for inhibiting the expression of dal, darl, upl3 and / or da2 in an oilseed plant, said method comprising: expressing at least one transgene encoding an inhibitory RNA molecule that inhibits expression of the dal, darl, upl3 and / or da2 in the oilseed plant, wherein the inhibitory RNA molecule comprises at least 25 consecutive nucleotides that have 90% or greater sequence identity to SEQ ID NO: 1, 3, 5, and / or 7, wherein expression of the dal, darl, upl3 and / or da2 is inhibited in the oilseed plant relative to an oilseed plant lacking the at least one transgene.

23. A method for producing a mutant oilseed plant, comprising the steps of:a) providing a first oilseed plant and a second oilseed plant, the first oilseed plant comprising a mutation in a dal, darl, upl3, and / or da2 nucleic acid sequence having a non-mutant sequence as set forth in SEQ ID NOs: 1, 3, 5, and / or 7, wherein the first oilseed plant exhibits a phenotype of increased seed size, increased seed oil content, and / or increased seed protein content and the second oilseed plant exhibits a desired phenotypic trait; b) crossing the first oilseed plant with the second oilseed plant to produce one or more Fi progeny plants; c) collecting seed produced by the Fi progeny plants; and d) germinating the seed to produce oilseed plants having a phenotype of increased plant size, increased seed size, increased seed weight, increased seed oil content, and / or increased seed protein content.

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