Synergistic pesticidal composition comprising insecticides and fungicide

Abstract

The present invention relates to synergistic pesticidal composition for the protection of various crops, plants against pests and plant diseases including leaf hoppers, Brown plant hopper (Nilparvata lugens), White backed plant hoppers (Sogatella furcifera), Green Leaf Hopper, whitefly, jassids, aphids, thrips, blast disease.

Description

[0001] SYNERGISTIC PESTICIDAL COMPOSITION COMPRISING INSECTICIDES AND FUNGICIDE

[0002] FIELD OF THE INVENTION

[0003] The present invention relates to synergistic pesticidal composition for the protection of various crops, plants against pests and plant diseases including leaf hoppers, Brown plant hopper (Nilparvata lugens), White backed plant hoppers (Sogatella furcifera), Green Leaf Hopper, whitefly, jassids, aphids, thrips, blast disease). More particularly, the present invention relates to a synergistic pesticidal composition comprising A) compound of Formula I, chemically known as ( lE)-N-[(6-chloro-3-pyridinyl)methyl]-N-ethyl-N'-methyl-2-nitro- 1 , 1 -ethenediamine or its agrochemically acceptable salts, esters and derivatives; B) at least one insecticidal compound selected from Dinotefuran and Pymetrozine or its agrochemically acceptable salts, esters and derivatives; and C) Isoprothiolane or its agrochemically acceptable salts, esters and derivatives for synergistic and effective control of various pests and plant diseases including leaf hoppers, Brown plant hopper (Nilparvata lugens), White backed plant hoppers (Sogatella furcifera), Green Leaf Hopper, whitefly, jassids, aphids, thrips, blast disease. The present invention also relates to a process for preparation of such pesticidal composition for synergistic and efficacious control of various pests including and plant diseases including leaf hoppers, Brown plant hopper (Nilparvata lugens), White backed plant hoppers (Sogatella furcifera), Green Leaf Hopper, whitefly, jassids, aphids, thrips, blast disease.

[0004] BACKGROUND OF THE INVENTION

[0005] Combination of pesticides are used to broaden the spectrum of control of pests and to improve the pest control with synergistic effect, reduce dosage, thereby reducing environmental impact, to broaden the spectrum of control, decrease chances of development and management of resistance and to enhance residual control so lesser the number of sprays for crop protections and minimizing the pesticidal load in ecosystem. The combination of pesticides at times demonstrate an additive or synergistic effect that results in an improved control on the pests.

[0006] Combination of insecticides and fungicides are used to broaden the spectrum of control of insect and fungal pests, reduce dosage, thereby reducing environmental impact, and decrease chances of development of resistance. The combination of insecticides and fungicides results in an improved control of the pests. Damage to seeds from insect and fungal pests is another major concern for agriculturalist. There are various diseases such as seed rot etc. which reduce germination rates and cause considerable decrease in yield. Treating the crops with insecticidal and fungicidal combinations helps reduce damage from both fungal diseases and insect pests. Another advantage of treating crops or specific plant propagation material is the improvement in germination rates, increased yield and improved plant health.

[0007] When fungicide resistance results from modification of a single major gene, pathogen subpopulations are either sensitive or highly resistant to the pesticide. Resistance in this case is seen as complete loss of disease control that cannot be regained by using higher rates or more frequent fungicide applications. This type of resistance is commonly referred to as "qualitative resistance." The control of phytopathogenic fungi and insect pests, which occur at the same time and at different stages of pest cycle, is of great economic importance since fungal and insect growth on plants or on parts of plants inhibits production of foliage, fruit or seed, and the overall quality of a cultivated crop.

[0008] The strobilurin, or Qol fungicides are extremely useful in controlling a broad spectrum of common vegetable pathogens. All strobilurin fungicides inhibit fungal respiration by binding to the cytochrome b complex III at the Q0 site in mitochondrial respiration. Simply said, the fungicide works by inhibiting the fungi’s ability undergo normal respiration. The strobilurin chemistries have a very specific target site, or mode-of-action (MOA). Although highly effective, fungicide chemistries like those in strobilurin fungicides with a very specific MOA, are susceptible to fungicide resistance development by some fungi. Therefore, proper fungicides rotations are necessary when fungicides with specific MOA’s are used in fungicide programs for controlling important diseases.

[0009] Hence, it is the need of the art to combine different systemic fungicide with preventive and curative action and which also act as plant growth regulator (PGR) to be used with specific insecticides for synergistic and efficacious control of various pests such as insect pests and fungi. PGRs help in increasing secretion of plant hormones and enzymes thereby activating root activity and promoting assimilation and translocation. These act as chemical messengers that facilitate intracellular communication. PGRs affect growth and differentiation of plants. More specifically, various PGRs can, for example stimulate seed germination, induce flowering, darken leaf coloring, minimize lodging of cereals, change the rate of plant growth, and modify the timing and efficiency of fruiting.

[0010] There are many pesticidal composition comprising fungicides and insecticides known in the art. There is however a need for improvement of such pesticidal compositions which are synergistic and equally effective, protective and having curative action to control various fungal diseases and insect pests. Single active compositions used over a long period of time has resulted in resistance. On account of such resistance development, more concentrated formulations are required to be applied resulting in various adverse effects on plant health and crop yield. With the onset of resistance to certain pests, there is a need in the art for a combination of actives that decreases chances of resistance and improves the spectrum of disease and pest control with improved crop yield and plant health.

[0011] There are many combinations of insecticides with fungicide known in the art, Reference can be made to CN 108522525 which discloses application of a composition containing Pyraclostrobin and Isoprothiolane, and relates to application of such composition in reducing or preventing infection of plants, plant propagation materials and plant organs, soil, materials or spaces grown later and storage objects by sigatoka or northern leaf blight fungus.

[0012] Further, reference can be made to CN102119687 which discloses a pesticidal composition containing Fluazinam and Isoprothiolane and application thereof, wherein the active ingredients of the bactericidal composition consist of the Fluazinam and the Isoprothiolane; and the mass ratio of the Fluazinam to the Isoprothiolane is 30: 1-1: 20.

[0013] Further, accordingly, there exists a need for combining fungicides and insecticides belonging to different chemical classes and showing different mechanism of action and useful for synergistic and effective control of various pests and diseases including leaf hoppers, Brown plant hopper (Nilparvata lugens), White backed plant hoppers (Sogatella furcifera), Green Leaf Hopper, whitefly, jassids, aphids, thrips, blast disease etc., at all stages of life cycle of the insect pests and fungi. The composition of the present invention is thus synergistic, reduces amount of pesticide used, is environmentally friendly, easy to formulate and is safe to use.

[0014] A compound of Formula I chemically known as (lE)-N-[(6-chloro-3-pyridinyl)methyl]-N-ethyl- N'-methyl-2-nitro- 1 , 1 -ethenediamine belongs from nitromethylene class. It acts as systemic insecticide with translaminar activity, stomach and contact action affecting insects nervous system and hence acts as nicotinic acetylcholine receptor (nAChR) competitive modulator. The chemical structure of compound formula I is provided below:

[0015] Dinotefuran is a nitroguanidine insecticide with characteristics of high efficiency, good root systemic property and the like, is widely used on crops such as rice, wheat, vegetables, fruit trees, tea leaves, cotton, tobacco and the like, is mainly used for preventing and control of pests such as aphids, bemisia tabaci, thrips, leafhoppers, liriomyza, leaf bees, mole crickets, coriaria, grain weevils, beetles, water wax insects, cockroaches. Dinotefuran mainly acts on an insect nervous system, enables the insects to be excited abnormally by combining with acetylcholine receptors, and finally causes general spasm and paralysis death. The chemical structure of compound Dinotefuran is provided below:

[0016]

[0017] Pymetrozine is a pyridine azomethine insecticide, and is widely used for controlling pests such as trialeurodes vaporariorum, leafhopper, cotton aphid and the like in crops due to excellent insecticidal effect. Pymetrozine has selective, neural inhibition of feeding behavior that eventually starves insect. It has chordotonal organ TRPV channel modulator. The chemical structure of compound Pymetrozine is provided below:

[0018] Isoprothiolane is a fungicide and a PGR used to control a range of rice pests such as Rice stem rot, Fusarium leaf spot, Planthoppers and diseases including Pyricularia oryzae, Helminthosporium sigmoideum and Fusarium nivale. The IUPAC name of Isoprothiolane is diisopropyl 1,3- dithiolan-2-ylidenemalonate and CAS name is bis( 1 -methylethyl) l,3-dithiolan-2- ylidenepropanedioate. It belongs from dithiolane class and is a systemic fungicide with curative and protective action. This compound is absorbed by the leaves and roots and translocate acropetally and basipetally. It has strong systemic activity and can be absorbed by each part of the crop / plant including paddy and accumulated to leaf tissues, thereby exerting the desired effect. It mainly prevents and treat rice blast having certain control effect on rice sheath blight disease, sclerotinia rot and bacterial leaf blight. The chemical structure of Isoprothiolane is provided below:

[0019] The present invention thus provides a synergistic pesticidal composition comprising A) compound of Formula I, chemically known as(lE)-N-[(6-chloro-3-pyridinyl)methyl]-N-ethyl-N'-methyl-2- nitro- 1,1 -ethenediamine or its agrochemically acceptable salts, esters and derivatives; B) at least one insecticidal compound selected from Dinotefuran and Pymetrozine or its agrochemically acceptable salts, esters and derivatives; and C) Isoprothiolane or its agrochemically acceptable salts, esters and derivatives for synergistic and effective control of various pests and plant diseases including leaf hoppers, Brown plant hopper (Nilparvata lugens), White backed plant hoppers (Sogatella furcifera), Green Leaf Hopper, whitefly, jassids, aphids, thrips, blast disease.

[0020] OBJECTIVES AND ADVANTAGES OF THE INVENTION

[0021] Accordingly, the main objective of the present invention is to provide a synergistic pesticidal composition for synergistic and effective control of various pests including fungi, insect pests and plant diseases including leafhoppers, Brown plant hopper (Nilparvata lugens), White backed plant hoppers (Sogatella furcifera), Green Leaf Hopper, whitefly, jassids, aphids, thrips, blast disease.

[0022] It is another objective of the present invention to provide improved, stable and ready to use pesticidal composition having synergistic and superior properties compared to prior art formulations or mixtures or compositions.

[0023] It is yet another objective of the present invention to provide a pesticidal composition for synergistic and effective control of various types of insects, fungi and plant diseases including brown plant hopper, whiteflies, jassids, aphids, thrips, white backed plant hopper, green leaf hopper and leaf folder, bugs, rice ear headed bugs, stem borer, sheath blight, leaf blast, powdery mildew, Anthracnose / Fruit Rot, frog eye leaf spot (Cercospora)& alternaria leaf spot, tikka disease, alternaria leaf blight, turcicum leaf blight, sigatoka leaf spot, anthracnose, purple blotch, early blight, late blight, early blight, septoria leaf spot, frog eye leaf spot, alternaria blight, powdery mildew, cercospora leaf spot, rust, collar rot, stem rot, loose smut, seed and seedling rot disease, glume discoloration (dirty panicle).

[0024] Yet another objective of the present invention is to provide a process for the preparation of a stable synergistic pesticidal composition comprising A) compound of Formula I, chemically known as(lE)-N-[(6-chloro-3-pyridinyl)methyl]-N-ethyl-N'-methyl-2-nitro- 1,1 -ethenediamine or its agrochemically acceptable salts, esters and derivatives; B) at least one insecticidal compound selected from Dinotefuran and Pymetrozine or its agrochemically acceptable salts, esters and derivatives; and C) Isoprothiolane or its agrochemically acceptable salts, esters and derivatives

[0025] Yet another objective of the present invention is to provide a stable synergistic pesticidal composition that promotes plant health, growth and increases plant, crop yield in the field.

[0026] Another objective of the present invention is to provide one shot application of novel and effective synergistic pesticidal composition for killing / controlling highly resistant pests - insect pests, fungi and plant diseases including brown plant hopper, whitefly, jassids, aphids, thrips, blast disease, early blight.

[0027] It is another objective of the present invention to provide a novel and effective synergistic pesticidal composition which is environmentally safe, possesses broad spectrum bio-efficacy, is non-phytotoxic.

[0028] It is yet another objective of the present invention to provide a novel and effective synergistic pesticidal composition, which has improved stability during the desired shelf life at various temperature and pH conditions. It is another objective of the present invention to provide a novel and effective stable synergistic pesticidal composition, which is economically more beneficial. of the Present Invention:

[0029] 1. The synergistic pesticidal composition of the present invention results in significantly lower incidence of Blast disease and BPH pest infestation in paddy crop as compared to the binary combinations, solo compositions and untreated check.

[0030] 2. The synergistic pesticidal composition of the present invention is significantly superior from its individual product in reducing the disease incidence in paddy crop.

[0031] 3. The synergistic pesticidal composition of the present invention provides improved grain yield as compared to the untreated control and binary and solo treatments applied in the paddy crop. Also, found economical with higher Cost: Benefit Ratio.

[0032] 4. The synergistic pesticidal composition shows no adverse impact on natural enemies and no phytotoxicity effect on paddy crop.

[0033] The synergistic pesticidal composition of present invention is highly effective in controlling disease and insect pest and increasing production of paddy at lower doses of the products in the combination, thus the composition of present invention is cost effective and safer to the environment.

[0034] SUMMARY OF THE INVENTION

[0035] Accordingly, the present invention relates to a synergistic pesticidal composition comprising: A) compound of Formula I, chemically known as(lE)-N-[(6-chloro-3-pyridinyl)methyl]-N-ethyl-N'- methyl-2-nitro- 1,1 -ethenediamine or its agrochemically acceptable salts, esters and derivatives; B) at least one insecticidal compound selected from Dinotefuran and Pymetrozine or its agrochemically acceptable salts, esters and derivatives; and C) Isoprothiolane or its agrochemically acceptable salts, esters and derivatives for synergistic and effective control of various pests and plant diseases including leaf hoppers, Brown plant hopper (Nilparvata lugens), White backed plant hoppers (Sogatella furcifera), Green Leaf Hopper, whitefly, jassids, aphids, thrips, blast disease. In one embodiment of the present invention, the synergistic pesticidal composition comprises: a) Compound of Formula I i.e. (lE)-N-[(6-chloro-3-pyridinyl)methyl]-N-ethyl-N'-methyl-2- nitro- 1,1 -ethenediamine or its agrochemically acceptable salts, esters and derivatives; b) at least one insecticidal compound selected from Dinotefuran and Pymetrozine or its agrochemically acceptable salts, esters and derivatives and c) Isoprothiolane or its agrochemically acceptable salts, esters and derivatives.

[0036] In yet another embodiment of the present invention, the synergistic pesticidal composition comprises: a) Compound of Formula I i.e. (lE)-N-[(6-chloro-3-pyridinyl)methyl]-N-ethyl-N'-methyl-2- nitro- 1,1 -ethenediamine, or its agrochemically acceptable salts, esters and derivatives; b) at least one insecticidal compound selected from Dinotefuran and Pymetrozine or its agrochemically acceptable salts, esters and derivatives; c) Isoprothiolane or its agrochemically acceptable salts, esters and derivatives. d) One or more agrochemically acceptable excipients.

[0037] In another embodiment of the present invention, the synergistic pesticidal composition comprises: a) Compound of Formula I i.e. (lE)-N-[(6-chloro-3-pyridinyl)methyl]-N-ethyl-N'-methyl-2- nitro- 1,1 -ethenediamine, or its agrochemically acceptable salts, esters and derivatives in the range of 0.1-80% by weight of the composition. b) at least one insecticidal compound selected from Dinotefuran and Pymetrozine or its agrochemically acceptable salts, esters and derivatives in the range of 0.1-80% by weight of the composition. c) Isoprothiolane or its agrochemically acceptable salts, esters and derivatives in the range of 0.1-80% by weight of the composition. d) One or more agrochemically acceptable excipients.

[0038] In one another preferred embodiment of the present invention, the synergistic pesticidal composition comprises: a) Compound of Formula I i.e. (lE)-N-[(6-chloro-3-pyridinyl)methyl]-N-ethyl-N'-methyl-2- nitro- 1,1 -ethenediamine, or its agrochemically acceptable salts, esters and derivatives in the range of 0.1-80% by weight of the composition. b) Dinotefuran or its agrochemically acceptable salts, esters and derivatives in the range of 0.1-80% by weight of the composition. c) Isoprothiolane or its agrochemically acceptable salts, esters and derivatives in the range of 0.1-80% by weight of the composition. d) One or more agrochemically acceptable excipients.

[0039] In one another embodiment of the present invention, the synergistic pesticidal composition comprises: a) Compound of Formula I i.e. (lE)-N-[(6-chloro-3-pyridinyl)methyl]-N-ethyl-N'-methyl-2- nitro- 1,1 -ethenediamine, or its agrochemically acceptable salts, esters and derivatives in the range of 0.1-80% by weight of the composition. b) Pymetrozine or its agrochemically acceptable salts, esters and derivatives in the range of 0.1-80% by weight of the composition. c) Isoprothiolane or its agrochemically acceptable salts, esters and derivatives in the range of 0.1-80% by weight of the composition. d) One or more agrochemically acceptable excipients.

[0040] In one more preferred embodiment of the present invention, Emulsifiable concentrate pesticidal composition comprising: a) Compound of Formula I i.e. (lE)-N-[(6-chloro-3-pyridinyl)methyl]-N-ethyl-N'-methyl-2- nitro- 1,1 -ethenediamine, or its agrochemically acceptable salts, esters and derivatives in the range of 0.1-50% by weight of the composition. b) at least one insecticidal compound selected from Dinotefuran and Pymetrozine or its agrochemically acceptable salts, esters and derivatives in the range of 0.1-50% by weight of the composition. c) Isoprothiolane or its agrochemically acceptable salts, esters and derivatives in the range of 0.1-50% by weight of the composition. In one more preferred embodiment of the present invention, Emulsifiable concentrate pesticidal composition comprising: a) Compound of Formula I i.e. (lE)-N-[(6-chloro-3-pyridinyl)methyl]-N-ethyl-N'-methyl-2- nitro- 1,1 -ethenediamine, or its agrochemically acceptable salts, esters and derivatives in the range of 10 % by weight of the composition. b) Dinotefuran or its agrochemically acceptable salts, esters and derivatives in the range of 2.3% by weight of the composition.

[0041] Isoprothiolane or its agrochemically acceptable salts, esters and derivatives in the range of 20 % by weight of the composition.

[0042] In one more preferred embodiment of the present invention, Emulsifiable concentrate pesticidal composition comprising: a) Compound of Formula I i.e. (lE)-N-[(6-chloro-3-pyridinyl)methyl]-N-ethyl-N'-methyl-2- nitro- 1,1 -ethenediamine, or its agrochemically acceptable salts, esters and derivatives in the range of 10 % by weight of the composition. b) Pymetrozine or its agrochemically acceptable salts, esters and derivatives in the range of 12% by weight of the composition. c) Isoprothiolane or its agrochemically acceptable salts, esters and derivatives in the range of 20 % by weight of the composition.

[0043] In one more preferred embodiment of the present invention, Suspo-emulsion pesticidal composition comprising: a) Compound of Formula I i.e. (lE)-N-[(6-chloro-3-pyridinyl)methyl]-N-ethyl-N'-methyl-2- nitro- 1,1 -ethenediamine, or its agrochemically acceptable salts, esters and derivatives in the range of 10 % by weight of the composition. b) Dinotefuran or its agrochemically acceptable salts, esters and derivatives in the range of 2.3% by weight of the composition. c) Isoprothiolane or its agrochemically acceptable salts, esters and derivatives in the range of 20 % by weight of the composition.

[0044] In one more preferred embodiment of the present invention, Suspo-emulsion pesticidal composition comprising: a) Compound of Formula I i.e. (lE)-N-[(6-chloro-3-pyridinyl)methyl]-N-ethyl-N'-methyl-2- nitro- 1,1 -ethenediamine, or its agrochemically acceptable salts, esters and derivatives in the range of 10 % by weight of the composition. b) Pymetrozine or its agrochemically acceptable salts, esters and derivatives in the range of 12% by weight of the composition. c) Isoprothiolane or its agrochemically acceptable salts, esters and derivatives in the range of 20 % by weight of the composition.

[0045] In one embodiment of the present invention, the present pesticidal composition is stable and has enhanced shelf life.

[0046] DETAILED DESCRIPTION OF THE INVENTION

[0047] Discussed below are some representative embodiments of the present invention. The invention in its broader aspects is not limited to the specific details and representative methods.

[0048] All technical and scientific terms used herein have the same meanings as commonly understood by someone ordinarily skilled in the art to which the present subject matter belongs.

[0049] The terminology used, in the present disclosure, is only for the purpose of explaining a particular embodiment and such terminology shall not be considered to limit the scope of the present disclosure. As used in the present disclosure, the forms "a,” "an," and "the" may be intended to include the plural forms as well, unless the context clearly suggests otherwise. Thus, for example, reference to a composition containing “a compound” includes a mixture of two or more compounds. It should also be noted that the term “or” is generally employed in its sense including “and / or” unless the content clearly dictates otherwise. The terms "comprises," "comprising," “including,” and “having,” are open ended transitional phrases and therefore specify the presence of stated features, integers, steps, operations, elements, but do not forbid the presence or addition of one or more other features, integers, steps, operations, elements, components, and / or groups thereof. The particular order of steps disclosed in the method and process of the present disclosure is not to be construed as necessarily requiring their performance as described or illustrated. It is also to be understood that additional or alternative steps may be employed. The term “active ingredient” (a.i.) or “active agent” used herein refers to that component of the composition responsible for control or killing of pest and disease control.

[0050] The term “crop” shall include a multitude of desired crop plants or an individual crop plant growing at a locus.

[0051] The term “synergistic”, as used herein, refers to the combined action of two or more active agents blended together and administered conjointly that is greater than the sum of their individual effects.

[0052] “Bioactive amounts” as mentioned herein means that amount which, when applied for treatment of crops, is sufficient to give effect in such treatment.

[0053] In an embodiment, the treatment may occur before sowing of the plant propagation material so that the sown material has been pre-treated with the combination. In particular, seed coating or seed pelleting are preferred in the treatment of the combinations according to the invention. As a result of the treatment, the active ingredients in the combination are adhered on to the seed and therefore available for pest and / or disease control.

[0054] The term “control” means to inhibit the ability of pests to survive, grow, feed and / or reproduce, or to limit the pests related damage or loss in crop plants. To “control” pests may or may not mean killing the insects although, it preferably means killing the pests.

[0055] The term “health of a plant” or “plant health” is defined as a condition of the plant and / or its products. As a result of the improved health; yield, plant vigour, quality and tolerance to abiotic or biotic stress is increased. As a result, the health of a plant is increased even in the absence of pest pressure.

[0056] In another embodiment, the present invention, provides a stable synergistic pesticidal composition for treatment of plant propagation materials such seeds, seedlings; and plants / or crops; preferably seeds. Furthermore, both in the presence and absence of biotic or abiotic stress factors, the health of a plant improves after treatment with the pesticidal composition. The above identified indicators for the health condition of a plant may be interdependent or they may result from each other. An increase in plant vigor for example result in an increased yield and / or tolerance to abiotic or biotic stress.

[0057] In another preferred embodiment of the invention, the yield of the treated plants is significantly improved.

[0058] In another preferred embodiment of the invention, the yield of the plants treated according to the method of the invention, is increased synergistically.

[0059] The “increased yield” of a plant, in particular for an agricultural, silvicultural and / or horticultural plant means that the yield of a product of the respective plant is increased by a measurable amount over the yield of the same product of the plant produced under the same conditions, but without the application of the present invention composition.

[0060] Increased yield can be characterized, among others, by the following improved properties of the plant like increased plant weight, increased plant height, increased biomass such as higher overall fresh weight (FW), increased number of flowers per plant, higher grain yield, more tillers or side shoots (branches), larger leaves, increased shoot growth, increased protein content, increased oil content, increased starch content, increased pigment content, increased leaf index. According to the present invention, the yield is increased as compared to the untreated control plants or plants treated with pesticides compositions other than the composition of the present invention.

[0061] The term “benefit: cost” (B:C) refers to a ratio used in a cost-benefit analysis to summarize the overall relationship between the relative costs and benefits of a proposed composition. If a treatment has a benefit-cost ratio greater than 1.0, the treatment is expected to deliver a positive and better outcome. A further indicator for the condition of the plant is the plant vigour. The plant vigour manifests in several aspects such as the general visual appearance. Improved plant vigour can be characterized, among others, by the following improved properties of the plant: improved vitality of the plant, improved plant growth, improved plant development, improved visual appearance, improved plant stand (less plant verse / lodging), improved emergence, enhanced root growth and / or more developed root system, enhanced nodulation, in particular rhizobia nodulation, bigger leaf blade, bigger size, increased plant weight, increased plant height, increased tiller number, increased number of side shoots, increased number of flowers per plant, increased shoot growth, increased root growth (extensive root system), increased yield when grown on poor soils or unfavourable climate, enhanced photosynthetic activity (e.g. based on increased stomatai conductance and / or increased CO2 assimilation rate), increased stomatai conductance, increased CO2 assimilation rate, enhanced pigment content (e.g. chlorophyll content), earlier flowering, earlier fruiting, earlier and improved germination, earlier grain maturity, improved self-defence mechanisms, improved stress tolerance and resistance of the plants against biotic and abiotic stress factors such as fungi, bacteria, viruses, insects, heat stress, cold stress, drought stress, UV stress and / or salt stress, less non-productive tillers, less dead basal leaves, less input needed (such as fertilizers or water), greener leaves, complete maturation under shortened vegetation periods, less fertilizers needed, less seeds needed, easier harvesting, faster and more uniform ripening, longer shelf-life, longer panicles, delay of senescence, stronger and / or more productive tillers, better extractability of ingredients, improved quality of seeds (for being seeded in the following seasons for seed production), better nitrogen uptake, improved reproduction, reduced production of ethylene and / or the inhibition of its reception by the plant.

[0062] Treatment of plants or crops with the pesticidal composition of the present invention, improves the plant vigour. In another embodiment of the invention, the plant vigour of the plants treated with the composition of the present invention increases synergistically.

[0063] The improvement of the plant vigour according to the present invention particularly means that the improvement of any one or several or all of the above mentioned plant characteristics are improved. Another indicator for the condition of the plant is the “quality” of a plant and / or its products.

[0064] In another preferred embodiment of the invention, the quality of the treated plant is increased.

[0065] In another preferred embodiment of the invention, the quality of the plants treated according to the method of the invention, is increased synergistically.

[0066] According to the present invention, enhanced quality means that certain plant characteristics such as the content or composition of certain ingredients, are increased or improved by a measurable or noticeable amount over the same factor of the plant produced under the same conditions. Improved properties of the plant or its product, can characterise improved quality like increased nutrient content, increased protein content, increased content of fatty acids, increased metabolite content, increased carotenoid content, increased sugar content, increased amount of essential amino acids, improved nutrient composition, improved protein composition, improved composition of fatty acids, improved metabolite composition, improved carotenoid composition, improved sugar composition, improved amino acids composition, improved or optimal fruit colour, improved leaf colour, higher storage capacity, higher process ability of the harvested products.

[0067] Another indicator for the condition of the plant is the plant’s tolerance or resistance to biotic and / or abiotic stress factors. Biotic and abiotic stress, especially over long periods of time can have harmful effects on plants. Biotic stress is caused by living organisms while abiotic stress is caused for example by environmental extremes.

[0068] One indicator for the condition of the plant is the yield. “Yield” is to be understood as any plant product of economic value that is produced by the plant such as grains, fruits, vegetables, nuts, grains, seeds, wood (e.g. in the case of silviculture plants) or even flowers (e.g. in the case of gardening plants, ornamentals). The plant products may in addition be further utilized and / or processed after harvesting. As per one embodiment, the active pesticidal compound as mentioned above to be used in the synergistic composition of the present invention, can be in the form of base or any salt form or ester form of the active which is known in the art.

[0069] Variations or modifications to the composition of this invention, within the scope of the invention, may occur to those skilled in the art upon reviewing the disclosure herein. Such variations or modifications are well within the spirit of this invention.

[0070] It has been observed that the composition of present invention not only has enhances bio-efficacy and provides synergistic effect but also gives control of pests and diseases caused by insect pests and fungi for a longer duration as compared to solo and binary formulations of insecticides and fungicides.

[0071] In accordance with an embodiment of the present invention, there is provided a synergistic pesticidal composition comprising active ingredients present in the weight % range as given below:

[0072] In one embodiment of the present invention, the synergistic pesticidal composition comprises: a) Compound of Formula I, i.e. (lE)-N-[(6-chloro-3-pyridinyl)methyl]-N-ethyl-N'-methyl-2- nitro- 1,1 -ethenediamine, or its agrochemically acceptable salts, esters and derivatives; b) at least one insecticidal compound selected from Dinotefuran and Pymetrozine or its agrochemically acceptable salts, esters and derivatives; c) Isoprothiolane or its agrochemically acceptable salts, esters and derivatives. d) One or more agrochemically acceptable excipients. In yet another embodiment of the present invention, the synergistic pesticidal composition comprises: a) Compound of Formula I, i.e. (lE)-N-[(6-chloro-3-pyridinyl)methyl]-N-ethyl-N'-methyl-2- nitro- 1,1 -ethenediamine, or its agrochemically acceptable salts, esters and derivatives in the range of 0.1-80% by weight of the composition. b) at least one insecticidal compound selected from Dinotefuran and Pymetrozine or its agrochemically acceptable salts, esters and derivatives in the range of 0.1-80% by weight of the composition. c) Isoprothiolane or its agrochemically acceptable salts, esters and derivatives in the range of 0.1-80% by weight of the composition. d) One or more agrochemically acceptable excipients.

[0073] In one another preferred embodiment of the present invention, the synergistic pesticidal composition comprises: a) Compound of Formula I i.e. (lE)-N-[(6-chloro-3-pyridinyl)methyl]-N-ethyl-N'-methyl-2- nitro- 1,1 -ethenediamine, or its agrochemically acceptable salts, esters and derivatives in the range of 0.1-80% by weight of the composition. b) Dinotefuran or its agrochemically acceptable salts, esters and derivatives in the range of 0.1-80% by weight of the composition. c) Isoprothiolane or its agrochemically acceptable salts, esters and derivatives in the range of 0.1-80% by weight of the composition. d) One or more agrochemically acceptable excipients.

[0074] In one another embodiment of the present invention, the synergistic pesticidal composition comprises: a) Compound of Formula I i.e. (lE)-N-[(6-chloro-3-pyridinyl)methyl]-N-ethyl-N'-methyl-2- nitro- 1,1 -ethenediamine, or its agrochemically acceptable salts, esters and derivatives in the range of 0.1-80% by weight of the composition. b) Pymetrozine or its agrochemically acceptable salts, esters and derivatives in the range of 0.1-80% by weight of the composition. c) Isoprothiolane or its agrochemically acceptable salts, esters and derivatives in the range of 0.1-80% by weight of the composition. d) One or more agrochemically acceptable excipients.

[0075] In one more preferred embodiment of the present invention, Emulsifiable concentrate pesticidal composition comprising: a) Compound of Formula I i.e. (lE)-N-[(6-chloro-3-pyridinyl)methyl]-N-ethyl-N'-methyl-2- nitro- 1,1 -ethenediamine, or its agrochemically acceptable salts, esters and derivatives in the range of 0.1-50% by weight of the composition. b) at least one insecticidal compound selected from Dinotefuran and Pymetrozine or its agrochemically acceptable salts, esters and derivatives in the range of 0.1-50% by weight of the composition. c) Isoprothiolane or its agrochemically acceptable salts, esters and derivatives in the range of 0.1-50% by weight of the composition.

[0076] In one more preferred embodiment of the present invention, Emulsifiable concentrate pesticidal composition comprising: a) Compound of Formula I i.e. (lE)-N-[(6-chloro-3-pyridinyl)methyl]-N-ethyl-N'-methyl-2- nitro- 1,1 -ethenediamine, or its agrochemically acceptable salts, esters and derivatives in the range of 10 % by weight of the composition. b) Dinotefuran or its agrochemically acceptable salts, esters and derivatives in the range of 2.3% by weight of the composition. c) Isoprothiolane or its agrochemically acceptable salts, esters and derivatives in the range of 20 % by weight of the composition.

[0077] In one more preferred embodiment of the present invention, Emulsifiable concentrate pesticidal composition comprising: a) Compound of Formula I i.e. (lE)-N-[(6-chloro-3-pyridinyl)methyl]-N-ethyl-N'-methyl-2- nitro- 1,1 -ethenediamine, or its agrochemically acceptable salts, esters and derivatives in the range of 10 % by weight of the composition. b) Pymetrozine or its agrochemically acceptable salts, esters and derivatives in the range of 12% by weight of the composition. c) Isoprothiolane or its agrochemically acceptable salts, esters and derivatives in the range of 20 % by weight of the composition.

[0078] In one more preferred embodiment of the present invention, Suspo-emulsion pesticidal composition comprising: a) Compound of Formula I i.e. (lE)-N-[(6-chloro-3-pyridinyl)methyl]-N-ethyl-N'-methyl-2- nitro- 1,1 -ethenediamine, or its agrochemically acceptable salts, esters and derivatives in the range of 10 % by weight of the composition. b) Dinotefuran or its agrochemically acceptable salts, esters and derivatives in the range of 2.3% by weight of the composition. c) Isoprothiolane or its agrochemically acceptable salts, esters and derivatives in the range of 20 % by weight of the composition.

[0079] In one more preferred embodiment of the present invention, Suspo-emulsion pesticidal composition comprising: a) Compound of Formula I i.e. (lE)-N-[(6-chloro-3-pyridinyl)methyl]-N-ethyl-N'-methyl-2- nitro- 1,1 -ethenediamine, or its agrochemically acceptable salts, esters and derivatives in the range of 10 % by weight of the composition; b) Pymetrozine or its agrochemically acceptable salts, esters and derivatives in the range of 12% by weight of the composition; and c) Isoprothiolane or its agrochemically acceptable salts, esters and derivatives in the range of 20 % by weight of the composition.

[0080] In one another embodiment of the present invention, agrochemically acceptable excipients are selected from the group comprising but not limited to emulsifiers, solvents, surfactant / dispersing agent, anti-freezing agent, anti-foaming agent, wetting agent, antimicrobial / anti-bacterial agent, thickening agent / thickener, fillers, adjuvants or mixtures thereof. Examples of emulsifiers used herein include but not limited to ionic emulsifier, non-ionic emulsifier or Castor oil or its ethoxylates, tall oil or its ethoxylates, Calcium alkyl benzene sulfonate or mixtures thereof and present in the range of 0.1 -20% by weight of the composition.

[0081] Examples of solvent used herein include but not limited to dimethyl formamide, heavy aromatic hydrocarbon, C-9 aromatic naptha solvent, naptha solvent, C-9 solvent, N methyl pyrollidone, Di methyl sulfoxide, N- alcohol, alkyl amide, vegetable oil, mineral oil, aromatic solvents, water or mixtures thereof and present in the range of 0.1-90% by weight of the composition.

[0082] Examples of surfactants / dispersing agent used herein include but not limited to Tristyrenated phenol ethoxylate, Polyarylphenyl ether phosphate, calcium alkyl benzene sulphonate, tristyryl phenol, ethylated / Acrylic Copolymer / Ethoxylated Tristryl phenol Sulphate, Naphthalene sulfonic acid, sodium salt condensate with formaldehyde, Poly methyl methacrylate, Ethoxylated oleyl cetyl alcohol, Polyalkelene glycol ether, Tristyrenated phenol ethoxylate, Ethoxylated Fatty alcohol, sodium polycarboxylate, sodium lignosulphonates, calcium ligno sulphate, sodium salt of lignosulphonc acid, Poly aryl phenyl ether phosphate, Tristyrylphenol Ethoxylate Amine salt of phosphate, tristyryl phenol ethylate, Acrylic Copolymer, Ethoxylated Tristryl phenol Sulphate, Naphthalene sulfonic acid, sodium salt condensate with formaldehyde, Ethoxylated oleyl cetyl alcohol, Polyalkelene glycol, naphthalene sulphonate formaldehyde condensates, tristyrylphenolethoxylate phosphate, sulfonated aromatic polymer, sodium salt, ethoxylated fatty alcohol, alkyl ethoxylates, EO-PO block copolymers, and graft copolymers or mixtures thereof and present in the range of 0.1-15% by weight of the composition.

[0083] Examples of anti-freezing agent used herein include but not limited to glycols, monoethylene glycol, diethylene glycol, polyethylene glycols, methoxypolyethylene glycols, propylene glycol, polypropylene glycols, polybutylene glycols, glycerine, ethylene glycol and mixtures thereof and present in the range of 0.1-10% by weight of the composition.

[0084] Examples of antifoaming agent used herein include but not limited to silicon emulsion based antifoam agents, Siloxane polyalkyleneoxide, trisiloxane ethoxylates, Polydimethyl siloxane and mixtures thereof and present in the range of 0.1-10% by weight of the composition. Examples of wetting agent used herein include but not limited to Sodium salt of sulphated product, sodium lauryl sulphate, sodium dioctylsulphosuccinate, Tristyrylphenolethoxylate non-ionic emulsifier, mixture of non-ionic surfactants & Alkoxylated Alcohol, Block copolymer, alkyl phenol ethoxylates, and aliphatic alcohol ethoxylates, their salts and mixtures thereof and present in the range of 0.1-10% by weight of the composition.

[0085] Examples of antimicrobial / anti-bacterial agent used herein include but not limited to propionic acid and its sodium salt; sorbic acid and its sodium or potassium salts; benzoic acid and its sodium salt, hydroxy benzoic acid sodium salt, methyl p-hydroxy benzoate, and biocide such as sodium benzoate, sodium o-phenyl phenate, l,2-benzisothiazoline-3-one, 2-methyl-4-isothiazolin-3-one, 5-chloro-2-methyl-4-isothiazolin-3-one, potassium sorbate, parahydroxy benzoates, Benzisothiazolin-3-one, Formaldehyde or mixtures thereof and present in the range of 0.01-10% by weight of the composition.

[0086] Examples of thickening agent used herein include but not limited to montmorillonite, bentonite clay, magnesium aluminium silicate, attapulgite, natural extracts of seeds and seaweeds, guar gum, locust bean gum, carrageenam, xanthan gum, alginates, polysaccharides, methyl cellulose, carboxymethyl cellulose (CMC), hydroxyethyl cellulose (HEC), bentonite clay, modified starches, poly acrylates, polyvinyl alcohol and polyethylene oxide or mixtures thereof and present in the range of 0.1-10% by weight of the composition.

[0087] Examples of filler (s) used herein include but not limited to Silicon Dioxide, bentonite clay, china clay, precipitated silica, kaolin, talcum powder, starch, urea formaldehyde resin and diatomaceous earth and mixtures thereof and present in the range of 0.1-90% by weight of the composition.

[0088] Examples of adjuvants used herein include but not limited to Silicone Ethoxylated Oil, Polyvinyl Pyrrolidone, Polyvinyl Alcohol or mixtures thereof and present in the range of 0.1-10% by weight of the composition.

[0089] In one another embodiment, the pesticidal composition of present invention can be applied to a plant / crop / soil by spraying, rubbing, atomizing, irrigating, evaporating, dusting, fogging, broadcasting, pouring, mist blowing, soil mixing, foaming, painting, spreading-on, drenching, dipping or drip irrigation, etc.

[0090] In yet another embodiment of the present invention, the present pesticidal composition can be applied to various crops and plants including Rice (Paddy), Cotton, Brinjal, Chilli, Ground nut (Seed Treatment), Tomato, cucurbits, lettuce, cabbage, carrots, cruciferous, Soybean, Cotton, Corn, Banana, Chilli, Bitter gourd, Grape, Apple, Onion, Cucumber, Potatoes, Grapes, Mango, pepper, Pomegranate, Black gram, Cumin, Green gram, Soybean, Coffee, Tea, Maize, Chilli, barley, oats, sorghum, sunflowers, turf and forage, sugar beet, beans, peas and ornamentals.

[0091] In yet another embodiment of the present invention, the present pesticidal composition provides synergistic and effective control of various pests and plant diseases including leaf hoppers, Brown plant hopper (Nilparvata lugens), White backed plant hoppers (Sogatella furcifera), Green Leaf Hopper, whitefly, jassids, aphids, thrips, blast disease.

[0092] The present invention also relates to a process for preparation of such pesticidal composition for synergistic and efficacious control of various pests and plant diseases including leaf hoppers, Brown plant hopper (Nilparvata lugens), White backed plant hoppers (Sogatella furcifera), Green Leaf Hopper, whitefly, jassids, aphids, thrips, blast disease.

[0093] In yet another embodiment of the present invention, the present synergistic pesticidal composition can be formulated as one or more of Capsule suspension (CS), Dispersible concentrate (DC), Dustable powder (DP), Powder for dry seed treatment (DS), Emulsifiable concentrate (EC), Emulsifiable granule (EG), Emulsion water-in-oil (EO), Emulsifiable powder (EP), Emulsion for seed treatment (ES), Emulsion oil-in-water (EW), Flowable concentrate for seed treatment (FS), Granules (GR), Micro-emulsion (ME), Oil-dispersion (OD), Oil miscible flowable concentrate (OF), Oil miscible liquid (OL), Oil dispersible powder (OP), Suspension concentrate (SC), Suspension concentrate for direct application (SD), Suspo-emulsion (SE), Water soluble granule (SG), Soluble concentrate (SL), Spreading oil (SO), Water soluble powder (SP), Water soluble tablet (ST), Ultra-low volume (ULV) suspension, Tablet (TB), Ultra-low volume (ULV) liquid, Water dispersible granules (WG), Wettable powder (WP), Water dispersible powder for slurry seed treatment (WS), Water dispersible tablet (WT), a mixed formulation of CS and SC (ZC) or a mixed formulation of CS and SE (ZE) or a mixed formulation of CS and EW (ZW). In one more preferred embodiment of the present invention, the synergistic pesticidal composition can be formulated as Emulsifiable concentrate or Suspo-emulsion formulation.

[0094] The synergistic pesticidal composition of the present invention are effective in controlling broad spectrum of pests in various plants / crops including but not limited to brown plant hopper, whiteflies, jassids, aphids, thrips, white backed plant hopper, green leaf hopper and leaf folder, bugs, rice ear headed bugs, stem borer, sheath blight, leaf blast, powdery mildew, Anthracnose / Fruit Rot, frog eye leaf spot (Cercospora)& alternaria leaf spot, tikka disease, alternaria leaf blight, turcicum leaf blight, sigatoka leaf spot, anthracnose, purple blotch, early blight, late blight, early blight, septoria leaf spot, frog eye leaf spot, alternaria blight, powdery mildew, cercospora leaf spot, rust, collar rot, stem rot, loose smut, seed and seedling rot disease, glume discoloration (dirty panicle), fodder beet, oil seed rape, pansy.

[0095] In further embodiment of the present invention, the synergistic pesticidal composition of the present invention further comprises one or more additional components including but not limited to e.g., protective colloids, adhesives, thixotropic agents, penetration agents, stabilisers, sequestering agents. More generally, the active materials can be combined with any solid or liquid carrier / additive, which complies with usual formulation techniques. An adjuvant used in the present invention is any material that is added to an agrochemical formulation to enhance or modify the performance of the formulation. An adjuvants used in the present invention to make it a safer to ecological environmental, having low toxicity and having no phytotoxicity effects on any part of the plant.The synergistic pesticidal composition of the present invention can also comprise or may be applied together and / or sequentially with further active compounds. These further compounds can be selected from fertilizers or micronutrient donors or microorganisms or other preparations that influence plant growth, such as inoculants (e.g. a strain of nitrogen -fixing bacteria), and plant inducers.

[0096] In an embodiment, the composition of the present invention may be combined with at least another active ingredient such as those selected from but not limited to herbicide, insecticide, fungicide, biological agent, plant growth activator, fertilizers or combinations thereof. In one embodiment, the composition according to the present invention acts synergistically to control pests and diseases in various crops.

[0097] Through the synergistic pesticidal composition of present invention, the number of applications to control wide range of pests appearing at the same time is minimised. The composition of present invention is highly safe to the user and to the environment. The composition offers the user a single homogenous application eliminating the need for tank mix. The composition also is cost-effective as it provides much greater simultaneous control and can be used in a variety of crops with a broader spectrum of protection.

[0098] In particular, it has now been found, surprisingly that, for example, the pesticidal activity of the composition of present invention, compared with the pesticidal activity of individual components, is synergistic. In fact, various advantageous properties associated with the compositions according to the invention, include but are not limited to: a broadening of the spectrum of pesticidal activity to other pests, for example to resistant strains; a reduction in the rate of application of the active ingredients; adequate control of the pests at a rate of application at which the individual compounds are not very effective, advantageous behaviour during formulating and / or upon application, improved stability, improved crop characteristics including crop yields, more developed root system, increase in plant health including height, improved plant vigor, less plant verse (lodging), and other advantages familiar to a person skilled in the art.

[0099] In an embodiment, the present invention provides a process for preparing the present novel synergistic pesticidal composition which may be modified accordingly by any person skilled in the art based on the knowledge of the manufacturing the formulation. However, all such variations and modifications are still covered by the scope of present invention

[0100] Although this invention has been disclosed in the context of certain preferred embodiments and examples, it will be understood by those skilled in the art that the present invention extends beyond the specifically disclosed embodiments to other alternative embodiments and / or uses of the invention and obvious modifications and equivalents thereof. Thus, from the foregoing description, it will be apparent to one of ordinary skill in the art that many changes and modifications can be made thereto without departing from the spirit or scope of the invention as set forth herein.

[0101] In order that the present invention may be more readily understood, reference will now be made, by way of example, to the following description. It will be understood that the specification and examples are illustrative but not limitative of the present invention and that other embodiments within the spirit and scope of the invention will suggest themselves to those skilled in the art. Other embodiments can be practiced that are also within the scope of the present invention. The following illustrations of examples are intended to illustrate a stable synergistic fungicidal composition, but in no way limit the scope of the present invention.

[0102] EXAMPLES

[0103] Representative chemical composition examples of synergistic pesticidal composition of the present invention, are provided as follows:

[0104] Compound of Formula I, referred herein pertains to (lE)-N-[(6-chloro-3-pyridinyl)methyl]-N- ethyl-N'-methyl-2-nitro- 1,1 -ethenediamine, or its agrochemically acceptable salts, esters and derivatives.

[0105] Example 1

[0106] Table 1

[0107]

[0108] Example 2

[0109] Compound of Formula 1 10% + DINOTEFURAN 2.3% + ISOPROTHIOLANE 20% EC

[0110] Manufacturing Process:

[0111] 1. Charged Solvent C9 into a clean and dry reactor followed with stirring .

[0112] 2. Further added technical of Isoprothiolane and mixed well to dissolve and get a clear solution.

[0113] 3. Further added solvent Dimethylformamide (DMF) and mixed well.

[0114] 4. Further added technical of compound of Formula I and technical of Dinotefuran and mixed well to dissolve.

[0115] 5. Further added non anionic emulsifier and mixed well to form an emulsifiable concentrate (EC).

[0116] 6. Filtered the above mentioned solution through sparkler filter and collect the filtrate into another clean and dry vessel.

[0117] 7. Pack the formulation in QA approved containers.

[0118] Stability Data :

[0119] Compound of Formula I 10% + Dinotefuran 2.3% + Isoprothiolane 20% EC

[0120]

[0121] Example 3

[0122] Compound of Formula 1 10% ISOPROTHIOLANE 20% + + PYMETROZINE 12% SE

[0123] Manufacturing Process:

[0124] Isoprothiolane EW phase a) Into a clean and dry reactor (Reactor-01) charged solvent Naptha and stirred. b) Added Isoprothiolane technical and mixed well to dissolve to get a clear solution. c) In another clean and dry vessel (Reactor-02) charged D M Water and added Tristyrenated phenol ethoxylate, Calcium alkylbenzene sulphonate, polyvinyl alcohol, Polydimethylsiloxane, and Propylene Glycol and mixed well to dissolve. d) Added the material from Reactor-01 to Reactor-02 under high speed mixing and then analysed the above Isoprothiolane EW to be used for further process.

[0125] B] Py metrozine and Compound of Formula I Mill base a) Into a clean and dry reactor (Reactor-03) charged D M water and stirred well. b) Added Propylene Glycol, Polydimethylsiloxane, 1 ,2-Benz-iso-thiozolane, Sodium Lignosulphonate, Sodium salt of lignosulphonic acid, Polymethylmethacrylate and A compound of Formula I and Pymetrozine technical. c) Mixed well to form a homogeneous slurry. d) Allowed to pass this slurry through bead mill to achieve a desired particle size. e) Further analyse the above prepared analysis, to be used for further process.

[0126] Compound of Formula 1 10% + Isoprothiolane 20% + Pymetrozine 12% SE a) Mixed both Isoprothiolane EW phase and Pymetrozine and Compound of Formula I Millbase. b) To this added xanthan gum and mixed well. c) Further, packed the formulation in QA approved containers.

[0127] 5

[0128] Stability Data and Specification 0 BIO-EFFICACY DATA Evaluation of synergistic effect of pesticidal composition of the present invention

[0129] A synergistic effect of two or more products exists whenever the action of an active ingredient combination is greater than the sum of the actions of the individual components. Synergism was calculated by using Colby’s method (Weeds, vol. 15 No. 1 (Jan 1967), pp. 20-2.

[0130] The synergistic action expected for a given combination of two active components can be calculated as follows:

[0131] XY

[0132] E = (X + Y) -

[0133] 100

[0134] The synergistic action expected for a given combination of three active components can be calculated as follows:

[0135] (XY+YZ+XZ) (XYZ)

[0136] E = (X + Y + Z) - - + -

[0137] 100 10000

[0138] Where:

[0139] E represents expected percentage of pesticidal control for the combination of the two or three actives ingredients at defined doses (for example equal to x, y and z, respectively).

[0140] X is the percentage of pesticidal control observed by the compound (Compound of Formula I) at a defined dose (equal to x).

[0141] Y is the percentage of pesticidal control observed by the compound (Pymetrozine / Dinotefuran) at a defined dose (equal to y).

[0142] Z is the percentage of pesticidal control observed by the compound (Isoprothiolane) at a defined dose (equal to z).

[0143] When the percentage of pesticidal control observed for the combination is greater than the expected percentage, there is a synergism effect.

[0144] Observed control (%)

[0145] Ratio = -

[0146] Expected control (%)

[0147] Ratio of O / E > 1 , synergism observed The present invention is illustrated by way of examples, the examples are meant for illustrative purposes and should not be construed as limiting.

[0148] Experiment for Synergistic activity of compositon of present invention:

[0149] Example 1- Details of experiment on paddy crop: Field experiment for synergistic activity of Compound of Formula I, Pymetrozine, Isoprothiolane for the control of Brown Plant Hopper (BPH) and Blast disease in paddy crop was conducted at farmer field, Karnal (Haryana).

[0150] To evaluate the synergistic effect, paddy (Variety: CSR 30) crop was sown in 5 m x 5 m plots maintaining 20 cm x 20 cm plant distance with three replications and in Randomized Block Design (RBD). The treatments were applied as foliar spray in the form of SE formulation @ 1250 ml / ha.

[0151] Each active component was applied twice in different ternary and binary combinations. Solo components were also taken for comparison. The treatment details are as under:

[0152] The treatments T1 to T28 were used as foliar application whereas no treatment was done for T29 control treatment. Two applications at 15 days interval were done of all treatments on the appearance of Insect and disease at experimental plots. Observations for Blast disease (causal organism Magnaporthe oryzae) was recorded at before spray, 14 days after 1stand 2ndapplication by observing 3 leave on 10 random plants per plot and per cent disease incidence was calculated. The observations for BPH (Nilaparvata lugens) population at before spray, 7 & 14 days after 1stand 2ndapplication on 10 random hills per plot. The per cent reduction in disease incidence and BPH population was calculated over control. Based on the per cent reduction at 14 days after second spray in disease incidence and BPH population the Expected control was calculated and compared with Observed control. The results of the trial have been presented here under in Table 1.

[0153] Table 1: Synergistic effect of combination product of the present invention (Compound of

[0154] Formula I + Pymetrozine + Isoprothiolane) against Blast disease and BPH in paddy crop

[0155] It is clearly evident from the data shown in above Table 1 for per cent Blast disease control and per cent BPH pest control in paddy crop that the ternary combination composition of the present invention comprising Compound of Formula I, Pymetrozine, Isopro thiolane i.e. T1 to T4 are synergistic for both disease and pest control with > 1 Colby’s Ratio. The binary combination of two insecticides (Compound of Formula I + Pymetrozine) T5 to T8 is compatible but not effective to control blast disease being insecticides. The binary combination of one fungicide and one insecticide (Compound of Formula I + Isoprothiolane and Pymetrozine + Isoprothiolane) T9 to T16 are less effective to control Blast disease and for BPH pest control as compared to ternary combination of Compound of Formula I + Pymetrozine + Isoprothiolane T1 to T4.

[0156] Example 2- Details of experiment on paddy crop:

[0157] Field experiment for synergistic activity of Compound of Formula I, Pymetrozine, Isoprothiolane for the control of Brown Plant Hopper (BPH) and Blast disease in paddy crop was conducted at farmer field, Karnal (Haryana). To evaluate the synergistic effect, paddy (Variety: CSR 30) crop was sown in 5 m x 5 m plots maintaining 20 cm x 20 cm plant distance with three replications and in Randomized Block Design (RBD) The treatments were applied as foliar spray in the form of EC formulation @ 1250 ml / ha. Each active component was applied twice in different ternary and binary combinations. Solo components were also taken for comparison. The treatment details are as under:

[0158] The treatments T1 to T28 were used as foliar application whereas no treatment was done for T29 control treatment. Two applications at 15 days interval were done of all treatments on the appearance of Insect and disease at experimental plots. Observations for Blast disease (causal organism Magnaporthe oryzae) was recorded at before spray, 14 days after 1stand 2ndapplication by observing 3 leave on 10 random plants per plot and per cent disease incidence was calculated. The observations for BPH (Nilaparvata lugens) population at before spray, 7 & 14 days after 1st and 2ndapplication on 10 random hills per plot. The per cent reduction in disease incidence and BPH population was calculated over control. Based on the per cent reduction at 14 days after second spray in disease incidence and BPH population the Expected control was calculated and compared with Observed control. The results of the trial have been presented here under in Table 2.

[0159] Table 2: Synergistic effect of combination product of the present invention (Compound of Formula I + Pymetrozine + Isoprothiolane) against Blast disease and BPH in paddy crop

[0160] It is clearly evident from the data shown in above Table 2 for per cent Blast disease control and per cent BPH pest control in paddy crop that the ternary combination composition of the present invention comprising Compound of Formula I, Pymetrozine, Isopro thiolane i.e. T1 to T4 are synergistic for both disease and pest control with > 1 Colby’s Ratio. The binary combination of two insecticides (Compound of Formula I + Pymetrozine) T5 to T8 is compatible but synergistic effect shown is low for the control of BPH, but not effective to control blast disease being insecticides. The binary combination of one fungicide and one insecticide (Compound of Formula I + Isoprothiolane and Pymetrozine + Isoprothiolane) T9 to T16 are less effective to control Blast disease and for BPH pest control as compared to ternary combination of Compound of Formula I + Pymetrozine + Isoprothiolane T1 to T4.

[0161] Example 3- Details of experiment on paddy crop: Field experiment for synergistic activity of Compound of Formula I, Dinotefuran, Isoprothiolane for the control of BPH pest and Blast disease in paddy crop was at farmer field, Karnal (Haryana).

[0162] To evaluate the synergistic effect, paddy (Variety: CSR 30) crop was sown in 5 m x 5 m plots maintaining 20 cm x 20 cm plant distance with three replications and in Randomized Block Design (RBD). The treatments were applied as foliar spray in the form of EC formulation @ 1500 ml / ha.

[0163] Each active component was applied twice in different ternary and binary combinations. Solo components were also taken for comparison. The treatment details are as under:

[0164] The treatments T1 to T28 were used as foliar application whereas no treatment was done for T29 control treatment. Two applications at 15 days interval were done of all treatments on the appearance of Insect and disease at experimental plots. Observations for Blast disease (causal organism Magnaporthe oryzae) was recorded at before spray, 14 days after 1stand 2ndapplication by observing 3 leave on 10 random plants per plot and per cent disease incidence was calculated. The observations for BPH (Nilaparvata lugens) population at before spray, 7 & 14 days after 1stand 2ndapplication on 10 random hills per plot. The per cent reduction at 14 days after second spray in disease incidence and BPH population was calculated over control. Based on the per cent reduction in disease incidence and BPH population the Expected control was calculated and compared with Observed control. The results of the trial have been presented here under in Table 3.

[0165] Table 3: Synergistic effect of combination product of the present invention (Compound of Formula I + Dinotefuran + Isoprothiolane) against Blast disease and BPH in paddy crop

[0166] It is clearly evident from the data shown in above Table 3 for per cent Blast disease control and per cent BPH pest control in paddy crop that the ternary combination composition of the present invention comprising Compound of Formula I, Dinotefuran, Isoprothiolane T1 to T4 are synergistic for both disease and pest control with > 1 Colby’s Ratio. The binary combination of two insecticides (Compound of Formula I + Dinotefuran) T5 to T8 is compatible but synergistic effect shown is low for the control of BPH, but not effective to control blast disease being insecticides. The binary combination of one fungicide and one insecticide (Compound of Formula I + Isoprothiolane and Dinotefuran + Isoprothiolane) T9 to T16 are less effective to control Blast disease and for BPH pest control as compared to ternary combination of Compound of Formula I + Dinotefuran + Isoprothiolane T1 to T4.

[0167] Experiment for Bio-efficacy evaluation of composition of present invention:

[0168] For the ternary and binary combination based on Compound of Formula I, Pymetrozine / Dinotefuran and Isoprothiolane evaluation under field conditions, the basic test composition as Compound of Formula I 10% + Pymetrozine 12% + Isoprothiolane 20% SE, Compound of Formula I 10% + Pymetrozine 12% + Isoprothiolane 20% EC and Compound of Formula I 10% + Dinotefuran 2.3% + Isoprothiolane 20% EC were manufactured specifically by the inventor. For ternary combination, Compound of Formula I 10% + Pymetrozine 12% / Dinotefuran 2.3% + Isoprothiolane 20% EC / SE was used and for binary combinations, Compound of Formula I 10% + Isoprothiolane 20% SE / EC and Pymetrozine 12% / Dinotefuran 2.3% + Isoprothiolane 20% SE / EC were used. The solo formulations of Compound of Formula I 10% EC / SE, Pymetrozine 12% / Dinotefuran 2.3% EC / SE and Isoprothiolane 20% EC / SE were used for comparison. The other formulations namely Pymetrozine 50 % WG and Isoprothiolane 40 % EC available in the market were also used for comparison.

[0169] Example 4: Evaluation of the combination product of the present invention on paddy

[0170] The synergistic composition of the present invention Compound of Formula I 10% + Pymetrozine 12% + Isoprothiolane 20% SE was evaluated on paddy crop variety CSR 30 at a farmer field, Kaithal. The crop sowing was done in Randomized Block Design (RBD) with three replications maintaining a distance of 20 cm x 20 cm between plants and rows.

[0171] The observations were recorded for the following objectives.

[0172] Objectives:

[0173] 1. Bio-efficacy evaluation against Blast disease on paddy crop

[0174] 2. Bio-efficacy evaluation against BPH on paddy crop

[0175] 3. Bio-efficacy evaluation based on per cent reduction in Blast disease control

[0176] 4. Bio-efficacy evaluation based on percent reduction in BPH population

[0177] 5. Bio-efficacy evaluation based on crop yield.

[0178] 6. Phytotoxicity evaluation on paddy crop

[0179] 7. Economics of treatments based on Cost: Benefit Ratio Treatment details:

[0180] Methodology: The treatments T1 to T17 and T19 were used as foliar application whereas no treatment was done for T18 i.e., control treatment. All treatments were applied at the time of initiation of pest in the experimental plots.

[0181] The observations for Blast disease (causal organism Magnaporthe oryzae) were recorded at before spray and 14 days after 1st& 2ndspray on three leaves per plant on 10 random plants per plot. The observations for BPH (Nilaparvata lugens) population at before spray and 7 & 14 days after 1st& 2ndspray on 10 random hills per plot. The per cent reduction in disease incidence and BPH population was also calculated over control. The crop yield was recorded at crop harvest. The observations for phytotoxicity on paddy crop at 1, 3, 7, 10 and 14 days after 1stspray were also recorded. Based on treatment application cost, market price of produce and net profit, the Cost:

[0182] Benefit Ratio was calculated for the economics of ternary combination treatments. The data were subjected to statistical analysis of variance. Results are presented in Table 4, 5, 6 and 7.

[0183] Results:

[0184] Table 4: Field bio-efficacy evaluation of Compound of Formula 1 10% + Pymetrozine 12% + Isoprothiolane 20% SE against blast of paddy crop

[0185] Figures in parentheses are angular transformed values; NS= Non significant

[0186] Table 5: Field bio-efficacy evaluation of Compound of Formula 1 10% + Pymetrozine 12% + Isoprothiolane 20% SE against BPH of paddy crop

[0187]

[0188] Figures in parentheses are square root transformed values; NS= Non significant

[0189] Table 6: Field bio-efficacy evaluation of Compound of Formula 1 10% + Pymetrozine 12% + Isoprothiolane 20% SE based on grain yield and Cost Benefit ratio of paddy crop.

[0190] DAS - Days after crop sowing Table 7: Phytotoxicity evaluation of Compound of Formula 1 10% + Pymetrozine 12% + Isoprothiolane 20% SE on paddy crop

[0191] From the example, it can be clearly seen that the combination of the present invention is superior for controlling the Blast disease (caused by Magnaporthe oryzae) at 14 days after 1st& 2ndspray (Table 4) and for controlling BPH Nilaparvata lugens) at 7 & 14 days after 1st& 2ndspray (Table 5) in paddy crop as compared to the other pesticides such as Pymetrozine 50 % WG and Isoprothiolane 40% EC available in the market. The yield of paddy crop also improved in the ternary combination of Compound of Formula I 10% + Pymetrozine 12% + Isoprothiolane 20% SE as compared to binary and solo compositions (Table 6). The Cost: Benefit Ratio revealed that ternary composition of Compound of Formula I 10% + Pymetrozine 12% + Isoprothiolane 20% SE @ 1250 ml / ha was most economical with higher Cost: Benefit Ratio as compared to other treatments (Table 6).

[0192] The disclosed compsoiton of Compound of Formula I 10% + Pymetrozine 12% + Isoprothiolane 20% SE @ 950ml, 1250 ml, 1600ml and 2500 ml / ha further showed no phytotoxicity in the paddy crop even after 1, 3, 7, 10 and 14 days after 1stspray (Table 7).

[0193] It is evident from the above data that the composition of the present invention i.e., Compound of Formula I 10% + Pymetrozine 12% + Isoprothiolane 20% SE resulted in good control of Blast disease and BPH in paddy with higher respective yields as compared to the reference standard products (binary compositions and solo products). The Cost: Benefit Ratio also showed that ternary composition of Compound of Formula I 10% + Pymetrozine 12% + Isoprothiolane 20% SE @ 1250 ml / ha was most economical with higher Cost: Benefit Ratio as compared to other binary and solo treatments. Further, the composition of present invention is resulted synergistic.

[0194] Example 5: Evaluation of the composition of the present invention on paddy

[0195] The synergistic composition of the present invention Compound of Formula I 10% + Pymetrozine 12% + Isoprothiolane 20% EC was evaluated on paddy crop variety CSR 30 at a farmer field, Kaithal. The crop sowing was done in Randomized Block Design (RBD) with three replications maintaining a distance of 20 cm x 20 cm between plants and rows.

[0196] The observations were recorded for the following objectives.

[0197] Objectives:

[0198] 1. Bio-efficacy evaluation against Blast disease on paddy crop

[0199] 2. Bio-efficacy evaluation against BPH on paddy crop

[0200] 3. Bio-efficacy evaluation based on per cent reduction in Blast disease control

[0201] 4. Bio-efficacy evaluation based on percent reduction in BPH population

[0202] 5. Bio-efficacy evaluation based on crop yield. 6. Phytotoxicity evaluation on paddy crop

[0203] 7. Economics of treatments based on Cost: Benefit Ratio

[0204] Treatment details: Methodology:

[0205] The treatments T1 to T17 and T19 were used as foliar application whereas no treatment was done for T18 i.e., control treatment. All treatments were applied at the time of initiation of pest in the experimental plots.

[0206] The observations for Blast disease (causal organism Magnaporthe oryzae) were recorded at before spray and 14 days after 1st& 2ndspray on three leaves per plant on 10 random plants per plot. The observations for BPH (Nilaparvata lugens) population at before spray and 7 & 14 days after 1st& 2ndspray on 10 random hills per plot. The per cent reduction in disease incidence and BPH population was also calculated over control. The crop yield was recorded at crop harvest. The observations for phytotoxicity on paddy crop at 1, 3, 7, 10 and 14 days after 1stspray were also recorded. Based on treatment application cost, market price of produce and net profit, the Cost: Benefit Ratio was calculated for the economics of ternary combination treatments. The data were subjected to statistical analysis of variance. Results are presented in Table 8, 9, 10, and 11. Results:

[0207] Table 8: Field bio-efficacy evaluation of Compound of Formula 1 10% + Pymetrozine 12% + Isoprothiolane 20% EC against blast of paddy crop

[0208] Figures in parentheses are angular transformed values; NS= Non significant

[0209] Table 9: Field bio-efficacy evaluation of Compound of Formula 1 10% + Pymetrozine 12% + Isoprothiolane 20% EC against BPH of paddy crop

[0210] Figures in parentheses are square root transformed values; NS= Non significant

[0211] Table 10: Field bio-efficacy evaluation of Compound of Formula 1 10% + Pymetrozine 12% + Isoprothiolane 20% EC based on grain yield and Cost Benefit ratio of paddy crop.

[0212] DAS - Days after crop sowing

[0213] Table 11: Phytotoxicity evaluation of Compound of Formula 1 10% + Pymetrozine 12% + Isoprothiolane 20% EC on paddy crop

[0214] From the example, it can be clearly seen that the combination of the present invention is superior for controlling the Blast disease (caused by Magnaporthe oryzae) at 14 days after 1st& 2ndspray (Table 8) and for controlling BPH (Nilaparvata lugens) at 7 & 14 days after 1st& 2ndspray (Table 9) in paddy crop as compared to the other pesticides such as Pymetrozine 50 % WG and Isoprothiolane 40% EC available in the market. The yield of paddy crop also improved in the ternary combination of Compound of Formula I 10% + Pymetrozine 12% + Isoprothiolane 20% EC as compared to binary and solo compositions (Table 10). The Cost: Benefit Ratio revealed that ternary composition of Compound of Formula I 10% + Pymetrozine 12% + Isoprothiolane 20% EC @ 1250 ml / ha was most economical with higher Cost: Benefit Ratio as compared to other treatments (Table 10).

[0215] The disclosed composition of Compound of Formula I 10% + Pymetrozine 12% + Isoprothiolane 20% EC @ 950ml, 1250 ml, 1600ml and 2500 ml / ha further showed no phytotoxicity in the paddy crop even after 1, 3, 7, 10 and 14 days after 1stspray (Table 11).

[0216] It is evident from the above data that the composition of the present invention i.e., Compound of Formula I 10% + Pymetrozine 12% + Isoprothiolane 20% EC resulted in good control of Blast disease and BPH in paddy with higher respective yields as compared to the reference standard products (binary compositions and solo products). The Cost: Benefit Ratio also showed that ternary combination Compound of Formula I 10% + Pymetrozine 12% + Isoprothiolane 20% EC @ 1250 ml / ha was most economical with higher Cost: Benefit Ratio as compared to other binary and solo treatments. Further, the composition of present invention is resulted synergistic.

[0217] Example 6: Evaluation of the combination product of the present invention on paddy

[0218] The synergistic composition of the present invention Compound of Formula I 10% + Dinotefuran 2.3% + Isoprothiolane 20% EC was evaluated on paddy crop variety CSR 30 at a farmer field, Karnal. The crop sowing was done in Randomized Block Design (RBD) with three replications maintaining a distance of 20 cm x 20 cm between plants and rows.

[0219] The observations were recorded for the following objectives.

[0220] Objectives:

[0221] 1. Bio-efficacy evaluation against Blast disease on paddy crop

[0222] 2. Bio-efficacy evaluation against BPH on paddy crop

[0223] 3. Bio-efficacy evaluation based on per cent reduction in Blast disease control

[0224] 4. Bio-efficacy evaluation based on percent reduction in BPH population

[0225] 5. Bio-efficacy evaluation based on crop yield.

[0226] 6. Phytotoxicity evaluation on paddy crop

[0227] 7. Economics of treatments based on Cost: Benefit Ratio

[0228] Treatment details:

[0229] Methodology: The treatments T1 to T17 and T19 were used as foliar application whereas no treatment was done for T18 i.e., control treatment. All treatments were applied at the time of initiation of pest in the experimental plots.

[0230] The observations for Blast disease (causal organism Magnaporthe oryzae) were recorded at before spray and 14 days after 1st& 2ndspray on three leaves per plant on 10 random plants per plot. The observations for BPH (Nilaparvata lugens) population at before spray and 7 & 14 days after 1st& 2ndspray on 10 random hills per plot. The per cent reduction in disease incidence and BPH population was also calculated over control. The crop yield was recorded at crop harvest. The observations for phytotoxicity on paddy crop at 1, 3, 7, 10 and 14 days after 1stspray were also recorded. Based on treatment application cost, market price of produce and net profit, the Cost:

[0231] Benefit Ratio was calculated for the economics of ternary combination treatments. The data were subjected to statistical analysis of variance. Results are presented in Table 12, 13, 14 and 15.

[0232] Results:

[0233] Table 12: Field bio-efficacy evaluation of Compound of Formula 1 10% + Dinotefuran 2.3% + Isopro thiolane 20% EC against blast of paddy crop

[0234] Figures in parentheses are angular transformed values; NS= Non significant

[0235] Table 13: Field bio-efficacy evaluation of Compound of Formula 1 10% + Dinotefuran 2.3% + Isoprothiolane 20% EC against BPH of paddy crop

[0236] Figures in parentheses are square root transformed values; NS= Non significant

[0237] Table 14: Field bio-efficacy evaluation of Compound of Formula 1 10% + Dinotefuran 2.3% + Isoprothiolane 20% EC based on grain yield and Cost Benefit ratio of paddy crop.

[0238] DAS - Days after crop sowing

[0239] Table 15: Phytotoxicity evaluation of Compound of Formula 1 10% + Dinotefuran 2.3% + Isoprothiolane 20% EC on paddy crop

[0240] From the example, it can be clearly seen that the combination of the present invention is superior for controlling the Blast disease (caused by Magnaporthe oryzae) at 14 days after 1st& 2ndspray (Table 12) and for controlling BPH Nilaparvata lugens) at 7 & 14 days after 1st& 2ndspray (Table 13) in paddy crop as compared to the other pesticides such as Dinotefuran 20 % SG and Isoprothiolane 40% EC available in the market. The yield of paddy crop also improved in the ternary combination of Compound of Formula I 10% + Dinotefuran 2.3% + Isoprothiolane 20% EC as compared to binary and solo compositions (Table 14). The Cost: Benefit Ratio revealed that ternary composition of Compound of Formula I 10% + Dinotefuran 2.3% + Isoprothiolane 20% EC @ 1500 ml / ha was most economical with higher Cost: Benefit Ratio as compared to other treatments (Table 14). The disclosed composition of Compound of Formula I 10% + Dinotefuran 2.3% + Isoprothiolane 20% EC @ 1125ml, 1500 ml, 1875 ml and 3000 ml / ha further showed no phytotoxicity in the paddy crop even after 1, 3, 7, 10 and 14 days after 1stspray (Table 15).

[0241] It is evident from the above data that the composition of the present invention i.e., Compound of Formula I 10% + Dinotefuran 2.3% + Isoprothiolane 20% EC resulted in good control of Blast disease and BPH in paddy with higher respective yields as compared to the reference standard products (binary compositions and solo products). The Cost: Benefit Ratio also showed that ternary composition of Formula I 10% + Dinotefuran 2.3% + Isoprothiolane 20% EC @ 1500 ml / ha was most economical with higher Cost: Benefit Ratio as compared to other binary and solo treatments. Further, the composition of present invention is resulted synergistic.

[0242] From the above investigations, it will be observed that numerous modifications and variations can be effectuated without departing from the true spirit and scope of the novel concepts of the present invention. It is to be understood that no limitations with respect to the specific embodiments illustrated is intended or should be inferred. It should be understood that all such modifications and improvements have been deleted herein for the sake of conciseness and readability but are properly within the scope of the following claims. From the above investigations, it will be observed that numerous modifications and variations can be effectuated without departing from the true spirit and scope of the novel concepts of the present invention. It is to be understood that no limitations with respect to the specific embodiments illustrated is intended or should be inferred. It should be understood that all such modifications and improvements have been deleted herein for the sake of conciseness and readability but are properly within the scope of the following claims.

[0243] It is to be understood that the present invention is susceptible to modifications, changes and adaptations by those skilled in the art. Such modifications, changes, adaptations are intended to be within the scope of the present invention.

Claims

I CLAIM

1. A synergistic pesticidal composition comprising: a) Compound of Formula I i.e. (lE)-N-[(6-chloro-3-pyridinyl)methyl]-N-ethyl-N'-methyl-2- nitro- 1,1 -ethenediamine or its agrochemically acceptable salts, esters and derivatives; b) at least one insecticidal compound selected from Dinotefuran and Pymetrozine or its agrochemically acceptable salts, esters and derivatives and c) Isoprothiolane or its agrochemically acceptable salts, esters and derivatives.

2. The synergistic pesticidal composition as claimed in claim 1, wherein composition comprises: a) Compound of Formula I i.e. (lE)-N-[(6-chloro-3-pyridinyl)methyl]-N-ethyl-N'-methyl-2- nitro- 1,1 -ethenediamine, or its agrochemically acceptable salts, esters and derivatives in the range of 0.1-40% by weight of the composition; b) at least one insecticidal compound selected from Dinotefuran and Pymetrozine or its agrochemically acceptable salts, esters and derivatives in the range of 0.1-20% by weight of the composition and c) Isoprothiolane or its agrochemically acceptable salts, esters and derivatives in the range of 0.1-40% by weight of the composition.

3. The synergistic pesticidal composition as claimed in claim 1-2, which further comprises one or more agrochemically acceptable excipients.

4. The synergistic pesticidal composition as claimed in claim 3, wherein the agrochemically acceptable excipients are selected from the group comprising emulsifiers, solvents, surfactant / dispersing agent, anti-freezing agent, anti-foaming agent, wetting agent,antimicrobial / anti-bacterial agent, thickening agent / thickener, fillers, adjuvants or mixtures thereof.

5. The synergistic pesticidal composition as claimed in claim 4, wherein the emulsifiers are selected from the group comprising ionic emulsifier, non-ionic emulsifier or Castor oil or its ethoxylates, tall oil or its ethoxylates, Calcium alkyl benzene sulfonate or mixtures thereof and present in the range of 0.1-20% by weight of the composition.

6. The synergistic pesticidal composition as claimed in claim 4, wherein the solvent is selected from the group comprising dimethyl formamide, heavy aromatic hydrocarbon, C-9 aromatic naptha solvent, naptha solvent, C-9 solvent, N methyl pyrollidone, Di methyl sulfoxide, N- alcohol, alkyl amide, vegetable oil, mineral oil, aromatic solvents, water or mixtures thereof and present in the range of 0.1-90% by weight of the composition.

7. The synergistic pesticidal composition as claimed in claim 4, wherein the surfactants / dispersing agent is selected from the group comprising Tristyrenated phenol ethoxylate, Polyarylphenyl ether phosphate, calcium alkyl benzene sulphonate, tristyryl phenol, ethylated / Acrylic Copolymer / Ethoxylated Tristryl phenol Sulphate, Naphthalene sulfonic acid, sodium salt condensate with formaldehyde, Poly methyl methacrylate, Ethoxylated oleyl cetyl alcohol, Polyalkelene glycol ether, Tristyrenated phenol ethoxylate, Ethoxylated Fatty alcohol, sodium polycarboxylate, sodium lignosulphonates, calcium ligno sulphate, sodium salt of lignosulphonc acid, Poly aryl phenyl ether phosphate, Tristyrylphenol Ethoxylate Amine salt of phosphate, tristyryl phenol ethylate, Acrylic Copolymer, Ethoxylated Tristryl phenol Sulphate, Naphthalene sulfonic acid, sodium salt condensate with formaldehyde, Ethoxylated oleyl cetyl alcohol, Polyalkelene glycol, naphthalene sulphonate formaldehyde condensates, tristyrylphenolethoxylate phosphate, sulfonated aromatic polymer, sodium salt, ethoxylated fatty alcohol, alkyl ethoxylates, EO-PO block copolymers, and graft copolymers or mixtures thereof and present in the range of 0.1-15% by weight of the composition.

8. The synergistic pesticidal composition as claimed in claim 4, wherein the anti-freezing agent is selected from the group comprising glycols, monoethylene glycol, diethylene glycol, polyethylene glycols, methoxypolyethylene glycols, propylene glycol, polypropylene glycols, polybutylene glycols, glycerine, ethylene glycol and mixtures thereof and present in the range of 0.1-10% by weight of the composition.

9. The synergistic pesticidal composition as claimed in claim 4, wherein the antifoaming agent is selected form the group comprising silicon emulsion based anti-foam agents, Siloxane poly alkyleneoxide, trisiloxane ethoxylates, Polydimethyl siloxane and mixtures thereof and present in the range of 0.1-10% by weight of the composition.

10. The synergistic pesticidal composition as claimed in claim 4, wherein the wetting agent is selected form the group comprising Sodium salt of sulphated product, sodium lauryl sulphate, sodium dioctylsulphosuccinate, Tristyrylphenolethoxylate non-ionic emulsifier, mixture of non-ionic surfactants & Alkoxy lated Alcohol, Block copolymer, alkyl phenol ethoxylates, and aliphatic alcohol ethoxylates, their salts and mixtures thereof and present in the range of 0.1-10% by weight of the composition.

11. The synergistic pesticidal composition as claimed in claim 4, wherein the antimicrobial / anti- bacterial agent is selected from the group comprising propionic acid and its sodium salt; sorbic acid and its sodium or potassium salts; benzoic acid and its sodium salt, hydroxy benzoic acid sodium salt, methyl p-hydroxy benzoate, and biocide such as sodium benzoate, sodium o-phenyl phenate, l,2-benzisothiazoline-3-one, 2-methyl-4-isothiazolin-3-one, 5-chloro-2-methyl-4- isothiazolin-3-one, potassium sorbate, parahydroxy benzoates, Benzisothiazolin-3-one, Formaldehyde or mixtures thereof and present in the range of 0.01-10% by weight of the composition.

12. The synergistic pesticidal composition as claimed in claim 4, wherein the thickening agent is selected from the group comprising montmorillonite, bentonite clay, magnesium aluminium silicate, attapulgite, natural extracts of seeds and seaweeds, guar gum, locust bean gum, carrageenam, xanthan gum, alginates, polysaccharides, methyl cellulose, carboxymethyl cellulose (CMC), hydroxyethyl cellulose (HEC), bentonite clay, modified starches, polyacrylates, polyvinyl alcohol and polyethylene oxide or mixtures thereof and present in the range of 0.1-10% by weight of the composition.

13. The synergistic pesticidal composition as claimed in claim 4, wherein the filler is selected from the group comprising Silicon Dioxide, bentonite clay, china clay, precipitated silica, kaolin, talcum powder, starch, urea formaldehyde resin and diatomaceous earth and mixtures thereof and present in the range of 0.1-90% by weight of the composition.

14. The synergistic pesticidal composition as claimed in claim 4, wherein the adjuvants are selected from group comprising Silicone Ethoxylated Oil, Polyvinyl Pyrrolidone, Polyvinyl Alcohol or mixtures thereof and present in the range of 0.1-10% by weight of the composition.

15. The synergistic pesticidal composition as claimed in claim 1, wherein the composition is formulated as Emulsifiable concentrate (EC), Micro-emulsion (ME), Capsule suspension (CS), Dispersible concentrate (DC), Dustable powder (DP), Powder for dry seed treatment (DS), Emulsifiable granule (EG), Emulsion water-in-oil (EO), Emulsifiable powder (EP), Emulsion for seed treatment (ES), Emulsion oil-in-water (EW), Flowable concentrate for seed treatment (FS), Granules (GR), Micro-emulsion (ME), Oil-dispersion (OD), Oil 20 miscible flowable concentrate (OF), Oil miscible liquid (OL), Oil dispersible powder (OP), Suspension concentrate (SC), Suspension concentrate for direct application (SD), Suspo-emulsion (SE), Water soluble granule (SG), Soluble concentrate (SL), Spreading oil (SO), Water soluble powder (SP), Water soluble tablet (ST), Ultra-low volume (ULV) suspension, Tablet (TB), Ultra-low volume (ULV) liquid,Water dispersible granules (WG), Wettable powder (WP), Water dispersible powder for slurry seed treatment (WS), Water dispersible tablet (WT), a mixed formulation of CS and SC (ZC) or a mixed formulation of CS and SE (ZE) and a mixed formulation of CS and EW (ZW).

16. A synergistic Emulsifiable concentrate pesticidal composition comprising: a) Compound of Formula I i.e. (lE)-N-[(6-chloro-3-pyridinyl)methyl]-N-ethyl-N'-methyl-2- nitro- 1,1 -ethenediamine, or its agrochemically acceptable salts, esters and derivatives in the range of 0.1-80% by weight of the composition. b) at least one insecticidal compound selected from Dinotefuran and Pymetrozine or its agrochemically acceptable salts, esters and derivatives in the range of 0.1-80% by weight of the composition. c) Isoprothiolane or its agrochemically acceptable salts, esters and derivatives in the range of 0.1-80% by weight of the composition.

17. A synergistic Suspo-emulsion pesticidal composition comprising: a) Compound of Formula I i.e. (lE)-N-[(6-chloro-3-pyridinyl)methyl]-N-ethyl-N'-methyl-2- nitro- 1,1 -ethenediamine, or its agrochemically acceptable salts, esters and derivatives in the range of 0.1-80% by weight of the composition. b) at least one insecticidal compound selected from Dinotefuran and Pymetrozine or its agrochemically acceptable salts, esters and derivatives in the range of 0.1-80% by weight of the composition. c) Isoprothiolane or its agrochemically acceptable salts, esters and derivatives in the range of 0.1-80% by weight of the composition.

18. The synergistic pesticidal composition as claimed in claim 16 and 17, whichfurther comprises one or more agrochemically acceptable excipients.

19. The synergistic pesticidal composition as claimed in claim 1-17 wherein the use of said composition exhibits improvement in plant health, vigour and yield.

20. A kit-of-parts comprising a plurality of components as claimed in claim 1 , wherein said plurality of components comprises a) Compound of Formula I i.e. (lE)-N-[(6-chloro-3-pyridinyl)methyl]-N-ethyl-N'-methyl-2- nitro- 1,1 -ethenediamine or its agrochemically acceptable salts, esters and derivatives; b) at least one insecticidal compound selected from Dinotefuran and Pymetrozine or its agrochemically acceptable salts, esters and derivatives and c) Isoprothiolane or its agrochemically acceptable salts, esters and derivatives.

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