Method for preparing seaweed dietary fiber by compound enzyme method and use

Abstract

A method for preparing a seaweed dietary fiber by a compound enzyme method and a use. In the method, seaweed is treated with an alkali, washed with water, and filtered to remove impurities, so as to obtain pre-treated seaweed; after being bleached and acidified, the pre-treated seaweed is washed with water and filtered to remove impurities, so as to obtain acidified seaweed; and a compound enzyme is added to the acidified seaweed, a reaction is carried out for 3-6 h at a temperature of 40-60°C and a pH of 6.0-8.0 so as to carry out enzymolysis on an insoluble fiber, then after enzyme inactivation treatment, drying and crushing are carried out. The method has the characteristics of high production efficiency and high raw material utilization rate; and the resulting product can be used as a colloidal additive, a thickening stabilizer or a dietary fiber supplement.

Description

A method for preparing seaweed dietary fiber using a composite enzyme method and its application Technical Field

[0001] The present invention relates to the technical field of seaweed dietary fiber, in particular to a method for preparing dietary fiber using a composite enzyme method and application thereof. Background Art

[0002] Seaweed is a general term for marine algae, including kelp, laver, wakame, and agar-agar. Common marine algae can be divided into red algae, brown algae, and green algae. Seaweed is rich in dietary fiber, seaweed polysaccharides, mannitol, amino acids, protein, vitamins, and trace elements such as potassium, iron, calcium, phosphorus, iodine, selenium, and cobalt. Dietary fiber is an edible plant ingredient that is not broken down by endogenous enzymes or digested and absorbed in the small intestine, but is partially or completely fermented in the large intestine. It has various physiological functions, including laxative effects, blood sugar regulation, and lipid reduction. Seaweed polysaccharides accelerate excretion and reduce the retention and absorption of harmful substances, helping to treat constipation, detoxify, enhance beauty, and prevent colorectal cancer. Mannitol is a potent diuretic used in medicine to reduce intracranial and intraocular pressure, and is used as a kidney medicine, dehydration medication, and sugar substitute. Therefore, seaweed, as a food, has benefits such as preventing constipation, detoxifying, enhancing beauty, and preventing colorectal cancer.

[0003] Seaweed contains a large amount of dietary fiber, which has become the "seventh nutrient" after sugar, protein, fat, water, minerals and vitamins. However, there are few reports on the industrial production of seaweed dietary fiber in China. Most of the production is done using terrestrial plant resources. Therefore, it is of great significance to expand the development and application of seaweed dietary fiber resources.

[0004] The processing methods for agar and carrageenan, thickening and stabilizing food additives, generally extract only the soluble hydrophilic colloid fraction (soluble dietary fiber), leaving the 30-40% or more insoluble dietary fiber contained in the seaweed completely discarded as waste. Because the extract is highly viscous, a large amount of perlite powder must be added as a filter aid during filtration. Consequently, the insoluble dietary fiber in the seaweed, when mixed with the perlite powder, becomes polluting sludge, destined for landfill or storage, unusable. This results in severe environmental pollution and a heavy economic burden for businesses.

[0005] Conventional seaweed fiber on the market is generally made by physically crushing the seaweed after it is dried. Although this method retains most of the nutrients in the seaweed, it contains a lot of impurities, has a rough taste, and is almost insoluble in water, making it unsuitable for addition to various food products.

[0006] Patent publication number CN105326057A discloses a method for preparing seaweed dietary fiber, characterized by: 1) treating Gracilaria algae with alkali using a high-temperature dilute alkali method; 2) acidifying the alkali-treated Gracilaria algae with hydrochloric acid or sulfuric acid; 3) extracting soluble dietary fiber and filter residue from the acidified Gracilaria algae, and performing a functional activation treatment on the soluble dietary fiber to prepare highly active Gracilaria dietary fiber; 4) extracting insoluble dietary fiber from the filter residue in step 3), and further bio-transforming it to prepare highly active Gracilaria dietary fiber.

[0007] However, the above method still has some shortcomings. After acidification, the seaweed is extracted and the soluble dietary fiber and the residue are separated. However, due to the lack of a gelatin boiling step, the soluble dietary fiber and agar remain in the intact algal cells. At the same time, the separation of insoluble dietary fiber mostly uses the plate filter press method, and the residue processing is complicated and tedious. Technical issues

[0008] The present invention aims to provide a method for producing dietary fiber using a composite enzyme method, which has the advantages of being environmentally friendly, having high production efficiency and high raw material utilization rate. The method adopts an enzymatic hydrolysis method to decompose and prepare seaweed fiber, thereby reducing energy consumption, maximizing the utilization of seaweed, and generating substantially no solid waste. Technical Solutions

[0009] To achieve the first objective above, the present application provides the following technical solution: a method for preparing seaweed dietary fiber using a composite enzyme method, comprising the following steps:

[0010] S1. Alkali treatment: The washed seaweed is immersed in an alkaline solution for alkaline treatment, and the alkaline solution is recovered after treatment;

[0011] S2. Washing: Soak the alkaline-treated seaweed in tap water until it is fully soaked, then drain and repeat the process until the wash solution becomes neutral.

[0012] S3. Bleaching: Soak the seaweed in a sodium hypochlorite solution for bleaching;

[0013] S4. Washing: Rinse the bleached seaweed with tap water until no noticeable odor remains.

[0014] S5. Acidification: The seaweed obtained in step S4 is immersed in an oxalic acid solution for acidification;

[0015] S6. Washing: Soak and wash the acidified seaweed in tap water, repeatedly washing until neutral;

[0016] S7. Grinding and homogenizing: Grinding the washed and acidified seaweed;

[0017] S8. Enzymatic hydrolysis: Add a certain amount of clean water, heat to the enzymatic hydrolysis temperature, then add the complex enzyme for enzymatic hydrolysis. After the enzymatic hydrolysis is completed, heat to inactivate;

[0018] S9. Drying and pulverizing: Drying and pulverizing the enzymatic hydrolyzate obtained in step S8 into powder.

[0019] More preferably, the alkaline solution in step S1 is one or both of NaOH and KOH, with a mass fraction of 1% to 10%, and the heating temperature is 50 to 85 degrees Celsius for 3 to 5 hours.

[0020] More preferably, the concentration of oxalic acid used in step S5 is 0.02%-1%.

[0021] More preferably, in step S7, the washed acidified algae are ground and crushed into a slurry.

[0022] More preferably, the amount of the complex enzyme added in step S8 is 1.5%-7% wt% on a dry basis.

[0023] More preferably, the complex enzyme in step S8 is one or more combinations of cellulase, pectinase, hemicellulase, endoglucanase, xylanase, papain and neutral protease; the enzymatic hydrolysis temperature is 40-60 degrees, and the pH is 4.0-8.0.

[0024] More preferably, the seaweed raw material is any one or more of Gracilaria, Laminaria or Eucheuma. Beneficial effects

[0025] The present invention provides an application of seaweed dietary fiber. The seaweed dietary fiber of the present application can replace colloidal additives such as agar and carrageenan. As a common food raw material, it can be used in health meal replacement products such as dairy products and meat products. It has the advantages of clean label, thickening, stability and low production cost.

[0026] The present invention provides application of the obtained seaweed dietary fiber as a thickening stabilizer.

[0027] The present invention provides application of the obtained seaweed dietary fiber as a dietary fiber supplement.

[0028] The present invention provides a method for preparing seaweed dietary fiber using a bio-enzymatic method, which has the advantages of being environmentally friendly, having high production efficiency, and high raw material utilization. Traditional seaweed processing techniques, such as the process for preparing agar from Gracilaria, mainly include alkali treatment, bleaching and acidification, gelatin boiling, filtration pressing, drying and crushing. Large amounts of acid and alkali are used, energy consumption is high, and a large amount of seaweed nutrients are wasted during the gelatin boiling process. At the same time, the amount of waste generated by the filtration residue mixed with the filter aid is large. The present invention uses enzymatic hydrolysis to decompose and prepare seaweed fiber, reducing energy consumption while maximizing the utilization of seaweed and generating substantially no solid waste. The method of the present invention has the characteristics of high production efficiency and high raw material utilization.

[0029] The seaweed dietary fiber obtained by the invention has the advantages of being rich in marine mineral elements, high in dietary fiber content, stable in colloid properties and low in cost. Modes for Carrying Out the Invention

[0030] The following examples further illustrate the present application in detail.

[0031] Example 1

[0032] S1 alkali treatment: Gracilaria was added to tap water, soaked and washed to remove the accompanying sediment and shell impurities; the washed seaweed was added 5% wt of sodium hydroxide solution, heated to 85 degrees and stirred for 4 hours;

[0033] S2. Washing: Soak the algae after alkali treatment in tap water, drain, and repeat this process until the washing solution becomes neutral. Then soak in clean water for 30 minutes.

[0034] S3. Bleaching: Immerse the algae in a 1% wt sodium hypochlorite solution at room temperature and stir for 30 min.

[0035] S4. Washing: Rinse the bleached algae with tap water until no noticeable odor remains.

[0036] S5. Acidification: Immerse the washed algae in a 1% wt oxalic acid solution at room temperature with stirring for 30 min.

[0037] S6. Washing: Soak the acidified algae in tap water and wash repeatedly until the water is neutral.

[0038] S7. Grinding and homogenizing: Add the cleaned, acidified algae to a grinder and grind until no visible particles remain.

[0039] S8. Enzymatic hydrolysis: Add a certain amount of water, heat to 50 degrees, then add 1.5% cellulase and 1.5% pectinase, stir and react for 3 hours, then add 1.5% papain and react for 3 hours. After the enzymatic hydrolysis is completed, heat to 95 degrees to inactivate it;

[0040] S9. Drying and pulverizing: The enzymatic hydrolyzate was dried at 60 degrees for one day, and pulverized into 60 mesh size by a pulverizer to obtain seaweed dietary fiber with a yield of 27.0%.

[0041] Example 2

[0042] S1 alkali treatment: Wash Eucheuma, add 7% wt potassium hydroxide solution, heat to 50 degrees, and stir for 5 hours;

[0043] S2. Washing: Soak the algae after alkali treatment in tap water, drain, and repeat this process until the washing solution becomes neutral. Then soak in clean water for 30 minutes.

[0044] S3. Bleaching: Soak the algae in 1% wt sodium hypochlorite solution at room temperature and stir for 30 minutes.

[0045] S4. Washing: Rinse the bleached algae with tap water until no noticeable odor remains.

[0046] S5. Acidification: Immerse the washed algae in a 1% wt oxalic acid solution at room temperature with stirring for 30 min.

[0047] S6. Washing: Soak the acidified algae in tap water and wash repeatedly until the water is neutral.

[0048] S7. Grind and homogenize: Add the cleaned, acidified algae to a grinder and grind until no obvious particles remain.

[0049] S8. Enzymolysis: Add a certain amount of water to make the material-liquid ratio 1:50, heat to 50 degrees, add 1% cellulase, stir and react for 3 hours, and heat to 95 degrees to inactivate after the enzymolysis is completed:

[0050] S9. Drying and pulverizing: The enzymatic hydrolyzate was dried at 60 degrees for one day, and pulverized into 60 mesh size by a pulverizer to obtain seaweed dietary fiber with a yield of 36.09%.

[0051] Example 3

[0052] The difference between this example and Example 1 is that the oxalic acid concentration in step (5) is 0.05% wt, and the enzymes used in the enzymatic hydrolysis in step (8) are 1.5% endo-β-glucanase, 1.5% pectinase and 1.5% papain, with a yield of 28.0%.

[0053] Example 4

[0054] The difference between this example and Example 3 is that the enzymes used in the enzymatic hydrolysis in step (8) are 3% cellulase, 3% pectinase and 1.5% papain, and the yield is 28.4%.

[0055] Example 5

[0056] The difference between this example and Example 1 is that the concentration of oxalic acid in step (5) is 0.05% wt and the yield is 32.5%.

[0057] Example 6

[0058] The difference between this embodiment and embodiment 1 is that the concentration of sodium hypochlorite in step (3) is 0.5% wt and the yield is 32.4%.

[0059] Example 7

[0060] The difference between this example and Example 1 is that the concentration of oxalic acid in step (5) is 0.025% wt and the yield is 28.8%.

[0061] Example 8

[0062] The difference between this example and Example 2 is that the enzyme used in step (6) is 1% cellulase, the enzymatic hydrolysis temperature is 60 degrees Celsius, and the yield is 39.9%.

[0063] Example 9

[0064] The difference between this example and Example 2 is that the material-liquid ratio during enzymatic hydrolysis in step (6) is 1:100, and the yield is 39.6%.

[0065] Example 10

[0066] The difference between this embodiment and embodiment 2 is that the concentration of potassium hydroxide in step (1) is 8% wt, and the yield is 38.2%.

[0067] Comparative Example 1

[0068] Comparative Example 1 is agar produced in Indonesia, and its production process is a traditional process, and the specific steps are as follows:

[0069] S1. Alkali treatment: Gracilaria raw material was added to tap water, soaked and washed to remove the accompanying sediment and shell impurities; the washed seaweed was immersed in an alkaline solution for alkaline treatment, and the alkali solution was recovered after treatment;

[0070] S2. Washing: Soak the algae after alkali treatment in tap water, drain, and repeat until the washing solution becomes neutral.

[0071] S3. Bleaching: Soak the algae in a sodium hypochlorite solution for 30 minutes.

[0072] S4. Washing: Rinse the bleached algae with tap water until no noticeable odor remains.

[0073] S5. Acidification: Immerse the cleaned algae in oxalic acid solution for 30 minutes.

[0074] S6. Washing: Soak the acidified algae in tap water and wash repeatedly until the water is neutral.

[0075] S7. Boiling the colloid: Boil the acidified algae for 1-2 hours to dissolve the colloid;

[0076] S8. Filtration of the gel: Filter the gel solution and the solid residue, and cool the upper solution to the gel;

[0077] S9. Drying and pulverization: The gel was compressed and dehydrated, then dried and pulverized to obtain agar powder with a yield of 12.8%;

[0078] Comparative Example 2

[0079] Comparative Example 2 is refined carrageenan produced in Indonesia, and its production process is a traditional process, and the specific steps are as follows:

[0080] S1. Alkali treatment: The Eucheuma raw material was added to tap water, soaked and washed to remove the accompanying mud and shell impurities; the washed seaweed was immersed in an alkaline solution for alkaline treatment, and the lye was recovered after treatment;

[0081] S2. Washing: Soak the algae after alkali treatment in tap water, drain, and repeat until the washing solution becomes neutral.

[0082] S3. Bleaching: Soak the algae in a sodium hypochlorite solution for 30 minutes.

[0083] S4. Washing: Rinse the bleached algae with tap water until no noticeable odor remains.

[0084] S5. Boiling the colloid: Boil the acidified algae for 1-2 hours to dissolve the colloid;

[0085] S6. Filtration of the gel: Filter the gel solution and the solid residue, and cool the upper solution to the gel;

[0086] S7. Drying and pulverization: The gel was compressed and dehydrated, then dried and pulverized to obtain refined carrageenan powder with a yield of 19.3%.

[0087] Comparative Example 3

[0088] Comparative Example 3 is crude carrageenan produced in Indonesia, and its production process is a traditional process, and the specific steps are as follows:

[0089] S1. Alkali treatment: The Eucheuma raw material was added to tap water, soaked and washed to remove the accompanying mud and shell impurities; the washed seaweed was immersed in an alkaline solution for alkaline treatment, and the alkali solution was recovered after treatment;

[0090] S2. Washing: Soak the algae after alkali treatment in tap water, drain, and repeat until the washing solution becomes neutral.

[0091] S3. Bleaching: Soak the algae in a sodium hypochlorite solution for 30 minutes.

[0092] S4. Washing: Rinse the bleached algae with tap water until no noticeable odor remains.

[0093] S5. Drying and pulverizing: Drying and pulverizing to obtain crude carrageenan powder with a yield of 33.7%.

[0094] The samples were prepared by the methods in the examples and comparative examples, and their properties were tested according to the following methods. The test results are shown in Table 1.

[0095] Table 1 Performance test table of products in embodiments and comparative examples

[0096] Item Gel strength g / cm3 Hardness g Elasticity Chewability Recovery force Cohesion Viscosity g·s Expansion force mL / g Example 1 12 0.5747 2.378 0.985 21.2488 0.168 0.2896 9.416 Example 2 2 6 2.578 445 5.10 10.99 1343 8.81 0.32 0.76 546 9.932 10.1 Example 3 2 13.01 839 38.838 0.845 115 8.29 0.62 0.249 2 53.1318.1Example 4140.7591476.170.589493.7761.2680.1973333.2248.3Example 5221.4213490.6310.8651023.2310.5420.254278.4837.3Example 6197.7941802.2190.778561.1180.6370.242297.6656.9Example 7254.6325 203.2980.8971550.510.4560.267344.1317.8Example 8298.5433781.5260.9913064.1130.3370.785338.75911Example 9270.9923686.4760.993109.4120.350.835339.9279.7Example 10257.3261652.9960.848898.3160. 5670.461535.86110.2Comparative Example 11155.7988441.8550.996446.710.3870.756243.6397.5Comparative Example 21185.976433.6830.9855395.4840.2540.826311.91511.4Comparative Example 3514.0137681.1390.9917311.8540.3880.944158.5998.7

[0097] Texture Property Measurement: The test method used was Texture Profile Analysis (TPA). The test conditions were: pre-test speed 0.5 mm / s, post-test speed 1.0 mm / s, and stroke 8 mm. Test indicators included hardness, elasticity, cohesiveness, and chewability. Hardness refers to the peak pressure during the probe's initial penetration of the sample; elasticity refers to the ratio of the sample's recovered height during the second compression to the initial deformation; cohesiveness refers to the negative area between the initial compression curve reaching zero force and the beginning of the second compression curve. Its unit is the product of force and time. Chewability represents the energy required to masticate a solid sample to a stable state for swallowing. It is related to hardness, cohesiveness, and elasticity, and is numerically equal to the product of hardness, cohesiveness, and elasticity. Cohesiveness is the relative resistance to deformation after the initial compression compared to the deformation after the second compression. It is expressed on the curve as the ratio of the positive work done during the two compressions and represents the internal adhesive strength of the sample. Resilience refers to the ratio of the elastic energy released by the sample during the return process of the initial compression to the energy consumed by the probe during compression.

[0098] Swelling force: Place 0.5 g of dry sample in a graduated cylinder and measure its volume. Then, add 20°C water, dilute to 10 mL, and shake well. Then, place the sample at 20°C for 24 hours, measure the volume of the wet sample in the graduated cylinder again, and calculate the swelling force. Swelling force (mL / g) = (volume of the expanded sample - volume of the dry sample) / sample weight.

[0099] Determination of dietary fiber content: The detection method is based on GB5009.88-2014 "Determination of dietary fiber in food" to determine total dietary fiber TDF and insoluble dietary fiber IDF. The soluble dietary fiber SDF content is obtained according to: TDF=SDF+IDF.

[0100] From the appearance of the obtained seaweed dietary fiber, its whiteness is close to that of refined carrageenan and agar, and it is a white powder, which is better than crude carrageenan and does not have the fishy smell of seaweed. The colloid after gelation is also white or light yellow, and its transparency is not as good as refined carrageenan, but similar to agar, which is better than the yellow-brown color of crude carrageenan and does not have the peculiar smell of crude carrageenan. This is because the insoluble dietary fiber in the obtained seaweed dietary fiber destroys the neat spatial structure of the colloid during gelation and reduces the content of the curd polysaccharide. However, on the other hand, the soluble dietary fiber and insoluble dietary fiber in the seaweed dietary fiber bring different physiological functions to carrageenan or agar. For example, the adhesion of the soluble dietary fiber can form a restrictive barrier in the small intestine, delaying the absorption of triglycerides and serum cholesterol, while also reducing bile and cholesterol concentrations, preventing gallstone formation. Insoluble dietary fiber can promote intestinal peristalsis, and can also wrap up excess sugar, fat and other digested and absorbed waste in the intestine and excrete them together with the intestine, reducing the chance of excess sugar, fat, etc. coming into contact with the intestinal mucosa, reducing the absorption of excess calories, and is more meaningful to health.

[0101] Combining the texture data of the embodiment and the comparative example in Table 1, it can be seen that the texture characteristics of the seaweed dietary fiber prepared by the method of the present application are quite different from those of traditional carrageenan and agar. Its hardness is significantly lower than that of agar and carrageenan, and its gel strength is between 100 and 300 g / cm 3 The gelling ability of the carrageenan is broad, and the viscosity is generally greater than that of carrageenan and agar. Elasticity is comparable to that of traditional agar-carrageenan. While each has its own strengths and weaknesses in resilience, most examples exhibit greater resilience than traditional carrageenan and agar. Expansion force is slightly lower than that of refined carrageenan, with some examples exhibiting greater strength than agar.

[0102] Combined with the above results, it can be shown that the seaweed dietary fiber obtained by this method not only has the colloidal properties of traditional carrageenan and agar, but also has the physical and chemical properties of soluble dietary fiber and insoluble dietary fiber, and has health benefits.

[0103] The powdered product obtained in the examples of the present invention contains a water-soluble hydrophilic colloid component (60-75%), which has the composition and gel properties of conventionally processed carrageenan and agar, making it suitable for use as a thickening and stabilizing agent. It also contains insoluble dietary fiber (20-30%), making it suitable as a dietary fiber supplement for various foods such as yogurt, meat products, weight loss meal replacement powders, soft candies, and nutritional supplements for the elderly. The resulting seaweed dietary fiber not only possesses the colloidal properties of traditional carrageenan and agar, but also possesses the physical and chemical properties of both soluble and insoluble dietary fibers, demonstrating certain health benefits.

[0104] The ratio of soluble dietary fiber to insoluble dietary fiber in the product obtained in the embodiment of the present invention is 2-4:1, which meets the optimal ratio of soluble dietary fiber to insoluble dietary fiber required for daily human digestion and absorption.

[0105] The product obtained in the embodiment of the present invention is white or milky white powder, and substantially retains a large amount of rich nutrients and trace elements contained in the original seaweed.

[0106] The yield of the powder product obtained in the embodiment of the present invention can reach 27.0%-32.4%. The yield of the powder product obtained in step 10) using Eucheuma as the raw material can reach 33.9%-39.9%.

[0107] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions and improvements made within the spirit and principles of the present invention should be included in the scope of protection of the present invention.

Claims

1. A method for preparing seaweed dietary fiber by a composite enzyme method, characterized in that: The following steps are involved: S1. Alkali treatment: immersing the washed seaweed in an alkaline solution for alkaline treatment, and recovering the alkaline solution after treatment; S2. Washing: Soak the alkaline-treated seaweed in tap water, drain the water, and repeat until the washing liquid is neutral; S3. Bleaching: soaking the seaweed in a sodium hypochlorite solution for bleaching; S4. Washing: Rinse the bleached seaweed with tap water until there is no obvious odor; S5. Acidification: The seaweed obtained in step S4 is immersed in an oxalic acid solution for acidification; S6. Washing: Soak and wash the acidified seaweed in tap water, and wash repeatedly until it becomes neutral; S7. Grinding and homogenizing: Grinding the washed and acidified seaweed; S8. Enzymolysis: add a certain amount of clean water, heat to the enzymolysis temperature, add compound enzyme for enzymolysis, and heat to inactivate after the enzymolysis is completed; S9. Drying and crushing: Drying and crushing the enzymatic hydrolyzate obtained in step S8 into powder.

2. The method for preparing seaweed dietary fiber by a composite enzyme method according to claim 1, characterized in that: The alkaline solution in step S1 is one or both of NaOH and KOH, with a mass fraction of 1%-10%, a heating temperature of 50-85 degrees, and a heating time of 3-5 hours.

3. The method for preparing seaweed dietary fiber by a composite enzyme method according to claim 1, characterized in that: The concentration of oxalic acid used in step S5 is 0.02%-1%.

4. The method for preparing seaweed dietary fiber by a composite enzyme method according to claim 1, characterized in that: In step S7, the washed acidified algae are ground and pulverized into a slurry.

5. The method for preparing seaweed dietary fiber by a composite enzyme method according to claim 1, characterized in that: The complex enzyme in step S8 is one or more combinations of cellulase, pectinase, hemicellulase, endoglucanase, xylanase, papain and neutral protease; the enzymolysis temperature is 40-60 degrees, and the pH is 4.0-8.

0.

6. The method for preparing seaweed dietary fiber by a composite enzyme method according to claim 1, characterized in that: The amount of the complex enzyme added in step S8 is 1.5%-7% wt on a dry basis.

7. The method for preparing seaweed dietary fiber by a composite enzyme method according to claim 1, characterized in that: The seaweed raw material is any one or more of Gracilaria, Laminaria japonica or Eucheuma truncatula.

8. Use of the seaweed dietary fiber prepared by the method according to claim 1 as a colloid additive.

9. Use of the seaweed dietary fiber prepared by the method according to claim 1 as a thickening stabilizer.

10. Use of the seaweed dietary fiber prepared by the method according to claim 1 as a dietary fiber supplement.