Method for evaluating skin temperature change according to application of skin care device

Abstract

The present invention relates to a method for evaluating a skin temperature change according to the application of a skin care device and, more specifically, to a method for evaluating a skin temperature change according to the application of a skin care device, the method comprising the steps of: separating skin tissue, attaching the skin tissue to a board, and then fixing an L-shaped probe connected to a thermometer; and applying the skin care device, measuring skin temperature, and then analyzing the measured skin temperature data. The evaluation method of the present invention can use the L-shaped probe to accurately check the actual depth of energy penetration in the skin, the increase and decrease in temperature in the skin, the maintenance time of temperature in the skin, and the like after applying the skin care device.

Description

Method for evaluating skin temperature changes according to the application of skin beauty devices

[0001] The present invention relates to a method for evaluating skin temperature changes due to application of a skin beauty device, and more particularly, to a method for evaluating skin temperature changes due to application of a skin beauty device, comprising the steps of: separating skin tissue, attaching it to a board, and then fixing an L-shaped probe connected to a thermometer; and measuring skin temperature after application of the skin beauty device, and then analyzing the measured skin temperature data.

[0002] The skin is a complex tissue that covers the surface of the human body and performs various functions, including temperature regulation, sensation, and excretion. It is a crucial organ that protects the internal tissues and maintains homeostasis. Human skin is largely composed of the epidermis and the dermis. The epidermis is stratified into the basement membrane, spinous layer, granular layer, and stratum corneum, and multiple layers of finally differentiated dead cells accumulate on the skin surface, performing a protective barrier function. The dermis is composed of the papillary dermis, which is rich in fibroblasts and microvessels, and the reticular dermis, which is rich in thick collagen fibers.

[0003] The skin care market is expanding, driven by the expansion of the anti-aging market and rising consumer interest and spending. In addition to anti-aging cosmetics, standalone skin care devices utilizing vibration, infrared, ultrasound, radiofrequency, and lasers are widely used to maximize skin care effects. These devices range from expensive, large-scale devices used in hospitals and clinics to low-cost, compact devices for personal use.

[0004] In the anti-aging market in particular, a major trend is favoring non-invasive or minimally invasive devices to minimize treatment costs, recovery time, and side effects. In line with this trend, many lifting lasers and home-use beauty devices are being developed. Most of these devices work by irradiating the skin with ultrasound or high-frequency lasers, generating heat in the dermis and inducing immediate contraction.

[0005] However, this method has the disadvantage of being difficult to accurately determine whether the desired penetration depth into the skin is reached, whether the desired temperature is reached within the desired time, and how long that temperature lasts.

[0006]

[0007] Accordingly, the inventors of the present invention completed the present invention by establishing a skin temperature change evaluation method that confirms the effectiveness of various skin beauty devices by measuring temperature changes not only in the epidermal layer but also within the skin (dermis layer, subcutaneous fat layer).

[0008] The problem to be solved by the present invention is to provide a method for evaluating skin temperature changes due to application of a skin beauty device, including the steps of separating skin tissue, attaching it to a board, and then fixing an L-shaped probe connected to a thermometer; and the steps of measuring skin temperature after applying a skin beauty device, and then analyzing the measured skin temperature data.

[0009] In order to solve the above problem, the present invention provides a method for evaluating skin temperature change according to application of a skin beauty device, including the steps of: separating skin tissue and attaching it to a board; fixing an L-shaped probe connected to a thermometer to the separated skin tissue; and applying a skin beauty device to the epidermis of the separated skin tissue, then measuring skin temperature by the L-shaped probe and analyzing the measured skin temperature data through an analysis unit of the thermometer.

[0010] The above skin tissue may be one or more types of skin tissue selected from the group consisting of mouse, rat, pig, and human.

[0011] The above board may be made of one or more materials selected from the group consisting of cork, plastic, silicone, rubber, wood, metal and acrylic.

[0012] The above-mentioned L-type probes can be 2 to 32 in number.

[0013] The above skin beauty device may be a medical device or a home beauty device.

[0014] The evaluation method of the present invention can accurately determine the actual depth of energy arrival in the skin, the increase and decrease in temperature within the skin, and the temperature retention time within the skin after application of a skin beauty device by using an L-shaped probe. In addition, since it can improve the accuracy of safety and efficacy evaluation of skin beauty devices, it can be usefully utilized in the pre-screening stage before clinical trials after skin beauty device development, and since it can provide accurate data on temperature changes to provide reliability, it can increase the usability of patents, papers, and skin beauty device advertisements.

[0015] Figure 1 shows the removal site of the skin tissue layer according to the skin temperature measurement site.

[0016] Figure 2 shows the fixed position of the L-shaped probe according to the skin temperature measurement site.

[0017] Figure 3 schematically illustrates a series of steps for fixing an L-shaped probe, applying a skin beauty device, measuring skin temperature by the L-shaped probe, and analyzing the measured skin temperature data through the analysis unit of the thermometer.

[0018] Figure 4 shows the insertion surface (A) and the penetration surface (B) when measuring the temperature within the dermal layer using a straight probe.

[0019] Figure 5 shows that the predicted scheme and the actual temperature measurement range are different when using a linear probe.

[0020] Figure 6 shows a cross-section when measuring temperature within the dermal layer using an L-shaped probe.

[0021] Figure 7 shows that the predicted plan and the actual confirmed temperature measurement range are consistent when using an L-shaped probe.

[0022] Figure 8 shows the results of comparing temperature measurement accuracy according to probe shape (I-shaped and L-shaped).

[0023] Figure 9A shows the results of an experiment conducted at 2.5 J, Figure 9B shows the results of an experiment conducted at 4.0 J, and Figure 9C shows the temperature change according to the shot for 2.5 and 4.0 J.

[0024] Figure 10 shows the results when the number of probes is changed, and the number N on the horizontal axis represents the number of probes.

[0025] The present invention constructs a skin temperature change evaluation method for confirming the effectiveness of various skin beauty devices by measuring temperature changes in the depth of the epidermal layer within the skin rather than the skin surface, as well as the dermal layer and subcutaneous fat layer, and experimentally verifies this.

[0026] Accordingly, the present invention relates to a method for evaluating skin temperature changes due to application of a skin beauty device, comprising, in one aspect, a step of separating skin tissue and attaching it to a board; a step of fixing an L-shaped probe connected to a thermometer to the separated skin tissue; and a step of applying a skin beauty device to the epidermis of the separated skin tissue, and then measuring skin temperature by the L-shaped probe and analyzing the measured skin temperature data through an analysis unit of the thermometer.

[0027] In the present invention, the step of separating the skin tissue and attaching it to the board may include flattening the separated skin tissue, placing it on the floor, and attaching a board under the skin tissue to support it so that it does not slip. The board may be made of cork, plastic, silicone, rubber, wood, metal, or acrylic, and is preferably a cork board, but is not limited thereto. In one embodiment of the present invention, the skin tissue is separated and attached to the cork board.

[0028] In the present invention, the skin tissue may be skin tissue isolated from a mouse, rat, pig, or human, but is not limited thereto. In one embodiment of the present invention, mouse skin, pig skin, or cadaver skin was used.

[0029] The separated skin tissue can be obtained at a depth of up to 150 um to the epidermis, up to 5 mm to the dermis, and up to about 5 cm to the subcutaneous fat layer, and preferably at a depth of 3 to 5 cm. In one embodiment of the present invention, the skin tissue was obtained at a depth of 5 cm and used. The separated skin tissue can be used after being washed, and distilled water, saline solution, or PBS (phosphate buffer saline) can be used for washing, and preferably PBS can be used.

[0030] After washing the separated skin tissue, a preprocessing step may be performed. The preprocessing step includes removing the subcutaneous fat layer. Depending on the area where actual temperature measurement is required, the subcutaneous fat layer may or may not be removed. For example, if only temperature measurement up to the epidermal layer is required, the dermis and subcutaneous fat layers may be removed. If the skin temperature within the dermis layer is to be measured, the subcutaneous fat layer may be removed. If the temperature measurement of the subcutaneous fat layer is required, the subcutaneous fat layer may not be removed (see Fig. 1).

[0031] In the present invention, in the step of fixing an L-shaped probe connected to a thermometer to the separated skin tissue, the L-shaped probe may be fixed to at least one selected from the group consisting of an epidermal layer, a dermal layer, and a subcutaneous fat layer of the separated skin tissue. In this case, if the dermal layer and the subcutaneous fat layer are removed in the preprocessing step, the L-shaped probe may be fixed to the epidermal layer, and if the subcutaneous fat layer is removed, the L-shaped probe may be fixed to the dermal layer. In addition, if the subcutaneous fat layer is not removed, the L-shaped probe may be fixed to the subcutaneous fat layer (see Fig. 2).

[0032] In the case of conventional straight probes, due to the fluid nature of skin tissue, they did not penetrate consistently to the desired penetration depth, resulting in differences in temperature measurement values. On the other hand, the L-shaped probe, due to its unique curved structure, can be accurately positioned at the desired depth of the skin, and it was confirmed that this enables stable temperature measurement at the target location during temperature measurement (see Example 1).

[0033] In the present invention, after applying a skin beauty device to the epidermis of the separated skin tissue, the step of measuring skin temperature using the L-shaped probe and analyzing the measured skin temperature data through the analysis unit of the thermometer is a sequential step. The measured skin temperature data may be analyzed and recorded through the analysis unit of the thermometer at least once every two seconds. In addition, the analysis may be performed after extension conversion through the software of the thermometer analysis unit during application of the skin beauty device. In one embodiment of the present invention, OMEGA software was used.

[0034] The above-mentioned skin beauty device may include not only medical devices used in hospitals and the like, but also home beauty devices that can be used at home. Specifically, it may be a radiofrequency (RF) device, an ultrasound device, such as a high intensity focused ultrasound (HIFU) device, or a laser device, but is not limited thereto. In one embodiment of the present invention, an experiment was conducted using a radiofrequency device.

[0035] The above L-shaped probe can be used to measure skin temperature, and at least two probes can be used to accurately measure skin temperature changes. If the number of probes exceeds 32, temperature measurement and temperature analysis through the analysis unit of the thermometer in the subsequent step may become inaccurate, so the number of L-shaped probes is preferably 2 to 32, and more preferably 2 to 8. In one embodiment of the present invention, experiments were conducted with 1, 2, 4, and 8 probes, and it was confirmed that while single-probe measurement is easily influenced by external factors, in the case of 2 to 8 probes, the error of the measurement value can be reduced and reliable data can be provided (see Example 3).

[0036]

[0037] Hereinafter, the composition and effects of the present invention will be described in more detail through examples. These examples are intended solely to illustrate the present invention, and the scope of the present invention is not limited by these examples.

[0038]

[0039] <Experimental Method>

[0040] 1. Skin tissue separation and pretreatment

[0041] Cadaver skin was used for the experiment, and skin measuring 5 x 5 x 5 cm (width x length x tissue depth) was obtained for each individual and used in the experiment.

[0042] The acquired skin was washed at least three times with PBS before use. Depending on the area of ​​the acquired skin that required temperature measurement, the subcutaneous fat layer was removed or not. Figure 1 shows the areas of skin tissue removed according to the area of ​​skin temperature measurement. For example, if only temperature measurement up to the epidermis was required, the dermis and subcutaneous fat layers were removed. If the skin temperature within the dermis layer was measured, the subcutaneous fat layer was removed. If the temperature measurement in the subcutaneous fat layer was required, the subcutaneous fat layer was not removed.

[0043]

[0044] 2. Fixing the L-shaped probe to the separated skin tissue

[0045] After pretreatment, the temperature under the skin was adjusted to approximately 30 to 35℃ using a warmer, and then two or more L-shaped probes (Thermocoupler K type, Teflon® Coating) connected to a digital thermometer (digital thermometer, OMEGA) were inserted into the skin at the desired depth of 2 x 2 x 0.2 cm (width x length x tissue depth) from the dermis to the epidermis at the same depth. Figure 2 shows the fixing positions of the L-shaped probes according to the skin temperature measurement site.

[0046]

[0047] 3. Skin temperature measurement and skin temperature analysis after applying the skin beauty device

[0048] After that, the skin temperature was measured by the L-shaped probe, and if it was determined that the temperature was stably recorded for more than 1 minute through the analysis unit of the digital thermometer, the skin beauty device was applied. Fig. 3 schematically shows a series of steps of fixing the L-shaped probe, applying the skin beauty device, measuring the skin temperature by the L-shaped probe, and analyzing the measured skin temperature data through the analysis unit of the thermometer.

[0049] Skin beauty devices are not limited to any specific category, as long as they are used on the skin, such as radiofrequency (RF) devices and high-intensity focused ultrasound (HIFU) devices, and there are no specific restrictions on the shape of the device. Furthermore, they can be directly or indirectly irradiated onto the skin. For the RF device, experiments were conducted with the same time interval and shots, but with different energies of 2.5 and 4.0 J.

[0050] When skin temperature is measured by the N-type probe, the measured skin temperature data is analyzed and recorded once every two seconds through the thermometer's analysis unit. Specifically, the extension is converted and then analyzed through the thermometer analysis unit's software (OMEGA software).

[0051]

[0052] [Example 1] Analysis experiment on the difference in temperature measurement range and temperature measurement accuracy according to probe shape

[0053] Fig. 4 shows the insertion surface (A) and the penetration surface (B) when measuring the temperature within the dermis using a straight probe, and it was confirmed that there was a difference between the expected plan (scheme) and the actual temperature measurement range when using a straight probe (Fig. 5). Fig. 6 shows a cross-section when measuring the temperature within the dermis using an L-shaped probe, and it was confirmed that the expected plan and the actual temperature measurement range were consistent when using an L-shaped probe (Fig. 7).

[0054]

[0055] In addition, the temperature measurement accuracy according to the probe shape is compared and shown in Fig. 8. As a result, in the case of the straight probe, it did not penetrate to the desired penetration depth consistently due to the fluid nature of the skin tissue, which resulted in differences in the temperature measurement values. On the other hand, in the case of the L-shaped probe, it was confirmed that it could be accurately positioned at the desired depth of the skin due to its unique curved structure, and this enabled stable temperature measurement at the target location during temperature measurement.

[0056]

[0057] [Example 2] Experiment on measuring internal human skin temperature using a skin beauty device

[0058] Figure 9A shows the results of an experiment conducted at 2.5 J, Figure 9B shows the results of an experiment conducted at 4.0 J, and Figure 9C shows the temperature change according to the shot for 2.5 and 4.0 J.

[0059] As a result, it was confirmed that the dermal temperature was maintained for more than 10 seconds before measuring the dermal temperature after applying high frequency, and that different graphs appeared depending on the high frequency conditions. Specifically, when 2.5 J was applied, it was confirmed that the average skin temperature was maintained constant, and in particular, it was maintained at the original dermal temperature of 28.3 to 29 ℃. On the other hand, when 4.0 J was applied, it was confirmed that the average skin temperature gradually increased.

[0060]

[0061] [Example 3] Temperature measurement experiment according to the number of probes

[0062] Figure 10 shows the results when the number of probes is changed, and the number N on the horizontal axis represents the number of probes.

[0063] As a result, it was confirmed that while single probe measurements are easily influenced by external factors, multiple probe measurements of 2 to 8 can provide reliable data by reducing errors by simultaneously measuring temperatures at multiple points, collecting data from various locations, and calculating average values.

[0064]

[0065] The present invention has demonstrated that skin temperature changes can be accurately assessed even under different skin care device conditions. This demonstrates that temperature changes occurring in various layers of the skin can be accurately and simultaneously measured using a variety of skin care devices.

Claims

1. Step of separating skin tissue and attaching it to the board; A step of fixing an L-shaped probe connected to a thermometer to the separated skin tissue; and A method for evaluating skin temperature changes due to application of a skin beauty device, comprising the steps of applying a skin beauty device to the epidermis of the separated skin tissue, measuring skin temperature by the L-shaped probe, and analyzing the measured skin temperature data through an analysis unit of the thermometer.

2. A method for evaluating skin temperature changes due to application of a skin beauty device, characterized in that in paragraph 1, the skin tissue is at least one type of skin tissue selected from the group consisting of mouse, rat, pig, and human.

3. A method for evaluating skin temperature change according to application of a skin beauty device, characterized in that in paragraph 1, the board is made of at least one material selected from the group consisting of cork, plastic, silicone, rubber, wood, metal, and acrylic.

4. A method for evaluating skin temperature changes according to application of a skin beauty device, characterized in that the number of the L-shaped probes in the first paragraph is 2 to 32.

5. A method for evaluating skin temperature changes according to application of a skin beauty device, characterized in that the skin beauty device in paragraph 1 is a medical device or a home beauty device.

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