Cosmetic composition comprising organoid culture medium

Abstract

The present invention relates to a cosmetic composition comprising an organoid culture medium. The cosmetic composition according to one aspect has excellent effects of anti-aging, moisturizing activity, hair growth promotion, hair root strengthening and the like by comprising an organoid culture medium, and thus the cosmetic composition according to one aspect can be effectively used in skin condition improvement and scalp improvement.

Description

Cosmetic composition comprising organoid culture medium

[0001] It relates to a cosmetic composition.

[0002] An organoid is an organ-specific cell aggregate created by re-aggregating or recombining cells isolated from stem cells or organ-derived cells using a three-dimensional culture method. Organoids can be utilized in two ways: as disease models leveraging their similarity to actual organs, and as regenerative therapies leveraging their tissue regeneration capabilities.

[0003] Meanwhile, organoid culture medium, a common medium used to differentiate organoids, has limited utility. Furthermore, no studies have been conducted on the application of organoid culture medium to skin cells to improve skin condition, strengthen hair roots, or promote hair growth.

[0004] One aspect is to provide a composition comprising an organoid culture medium.

[0005] Another aspect is to provide a cosmetic composition for improving skin condition comprising an organoid culture fluid.

[0006] Another aspect is to provide a health functional food composition for improving skin condition, which includes an organoid culture fluid.

[0007] Another aspect is to provide a cosmetic composition for strengthening hair roots comprising an organoid culture solution.

[0008] Another aspect is to provide a cosmetic composition for preventing hair loss or promoting hair growth, comprising an organoid culture.

[0009] Another aspect provides the use of organoid cultures for the preparation of cosmetic compositions for improving skin condition.

[0010] Another aspect provides the use of organoid culture fluid for the preparation of a health functional food composition for improving skin condition.

[0011] Another aspect provides the use of organoid cultures for the preparation of cosmetic compositions for strengthening hair roots.

[0012] Another aspect provides the use of an organoid culture for the preparation of a cosmetic composition for preventing hair loss or promoting hair growth.

[0013] Another aspect provides a method for improving a skin condition comprising administering an effective amount of an organoid culture to a subject in need thereof.

[0014] Another aspect provides a method for strengthening hair roots comprising administering an effective amount of an organoid culture to a subject in need thereof.

[0015] Another aspect provides a method for preventing hair loss or promoting hair growth comprising administering an effective amount of an organoid culture to a subject in need thereof.

[0016] One aspect provides a composition comprising an organoid culture medium.

[0017] In this specification, the term "organoid culture medium" may be used interchangeably with "organoid culture supernatant", "organoid conditioned medium" or "organoid culture medium", and may mean the entire medium including the organoids, their metabolites, extra nutrients, etc., obtained by culturing the cells isolated from stem cells or organ-derived cells for a certain period of time in a medium capable of supplying nutrients so that the cells can grow and aggregate or recombine in three dimensions in vitro.

[0018] In one specific example, the organoid culture medium may include at least one component selected from the group consisting of GLUTAMATE 1, B-27, N2, 2-Mercaptoethanol, and Normocin in the basic medium.

[0019] The above basic medium may be Advanced D-MEM / F-12, Neurobasal, or a mixture thereof.

[0020] Additionally, the organoid culture medium may refer to a culture medium obtained by culturing the cells and removing organoids.

[0021] Meanwhile, the liquid from which organoids have been removed from the culture medium is also called a "supernatant." It can be obtained by allowing the culture medium to stand for a certain period of time and taking only the liquid from the upper layer excluding the portion that has settled to the lower layer, removing organoids through filtration, or centrifuging the culture medium to remove the sediment at the bottom and taking only the liquid at the upper layer.

[0022] The above stem cells are cells with self-renewal, differentiation, and self-organization functions, and may be, for example, adult stem cells (ASCs), embryonic stem cells (ESCs), or induced pluripotent stem cells (iPSCs). In addition, cells isolated from the organ-derived cells may be, for example, blood, skin tissue, adipose tissue, tonsils, etc.

[0023] In one specific example, the organoid culture may be obtained before culturing cells isolated from stem cells or organ-derived cells.

[0024] In another specific example, the organoid culture may be obtained after culturing cells isolated from stem cells or organ-derived cells. The organoid culture obtained after culturing the cells may contain metabolites of the organoids. Furthermore, these metabolites may exhibit synergistic effects in improving skin condition, strengthening hair roots, preventing hair loss, or promoting hair growth.

[0025] In one embodiment, it was confirmed that the expression of moisturizing factors (AQP3 and HAS3) increased in human epidermal cells treated with organoid cultures obtained before or after culturing cells isolated from stem cells or organ-derived cells, and the expression of skin elasticity and wrinkle-related factors (COL1A1 and FBN1) increased in human fibroblasts. In addition, in another embodiment, it was confirmed that the proliferation of dermal papilla cells was induced in the organoid cultures, and the expression of hair growth-related factors (VEGF, FGF7, and HGF) increased.

[0026] Accordingly, the composition according to one aspect may have effects that improve skin condition, such as anti-aging, wrinkle improvement, moisturizing, strengthening the skin barrier, or improving elasticity. Furthermore, the composition may have effects that strengthen hair roots, prevent hair loss, or promote hair growth.

[0027] The term "skin aging" in this specification refers to a general term for tangible and intangible changes that occur in the skin with age, such as thinning of the epidermis, decreases in the number of cells or blood vessels in the dermis, DNA damage repair ability, cell turnover cycle, wound healing, skin barrier function, moisture retention in the epidermis, sweat secretion, sebum secretion, vitamin D production, physical damage defense, chemical removal ability, immune response, sensory function, and temperature regulation.

[0028] The above fermented product may be used for improving skin aging caused by exogenous or endogenous factors. The exogenous factors refer to various external factors, such as ultraviolet rays (light), and the endogenous factors are also referred to as chronological factors and mainly refer to factors that occur over time. In other words, the above skin aging includes not only premature aging symptoms induced by external stimuli such as ultraviolet rays, pollution, cigarette smoke, and chemicals, but also natural aging phenomena that occur due to a decrease in skin cell proliferation with age, and is a concept that includes wrinkles, loss of elasticity, sagging skin, and dryness. In addition, wrinkles include stimuli caused by changes in internal and external factors that change the components that make up the skin tissue, thereby causing wrinkles.

[0029] The aforementioned aging may be photoaging. The term "photoaging" refers to a phenomenon caused by external environmental factors, the most common of which is ultraviolet rays. Ultraviolet rays cause damage to biological components, including the activation of proteolytic enzymes, chain scission of substrate proteins, and abnormal cross-linking. The repetition of these mechanisms leads to visible skin aging.

[0030] As used herein, the term "wrinkle" refers to a state in which skin loses elasticity and becomes loose, such as when the skin folds. Pigmentation refers to a condition in which the amount of pigment in the body is abnormal or where pigment appears abnormally, such as when freckles or spots appear. The term "prevention or improvement of skin wrinkles" may refer to any action that prevents or improves wrinkles by suppressing the expression of factors related to wrinkles, or increases the total amount of collagen.

[0031] The above “strengthening the skin barrier” may refer to any action that enhances the function of the skin barrier, which is located at the outermost part of the skin and prevents moisture and nutrient loss.

[0032] The above “skin moisturizing” may mean any action that maintains skin moisture or prevents moisture loss.

[0033] The above “skin regeneration” may mean any action that replenishes a part of the skin that has been lost or damaged, or promotes the proliferation and movement of skin cells.

[0034] The composition is comprised of 0.001 wt% to 80 wt%, for example, 0.01 wt% to 60 wt%, 0.01 wt% to 40 wt%, 0.01 wt% to 30 wt%, 0.01 wt% to 20 wt%, 0.01 wt% to 10 wt%, 0.01 wt% to 5 wt%, 0.05 wt% to 60 wt%, 0.05 wt% to 40 wt%, 0.05 wt% to 30 wt%, 0.05 wt% to 20 wt%, 0.05 wt% to 10 wt%, 0.05 wt% to 5 wt%, 0.1 wt% to 60 wt%, 0.1 wt% to 40 wt%, 0.1 wt% to 30 wt%, 0.1 wt% to 20 The organoid culture solution may be included in an amount of 0.1 wt% to 10 wt%, or 0.1 wt% to 5 wt%. In this case, if the content of the organoid culture solution is less than the above range, there is a problem in that the skin condition improvement effect, such as anti-aging and moisturizing, or hair root strengthening effect is not sufficiently exerted.

[0035] In this specification, the term "included as an active ingredient" means that the organoid culture solution of this specification is added to an extent that can exhibit the above-mentioned effect, and includes formulation in various forms by adding various components as auxiliary components for drug delivery and stabilization, etc.

[0036] In one specific example, the composition may be a cosmetic composition.

[0037] The above cosmetic composition may have, for example, a cosmetic formulation of a softening toner, a nourishing toner, a massage cream, a nourishing cream, an essence, a pack, a gel, an ampoule, or a skin adhesive type.

[0038] The ingredients included in the above cosmetic composition may include ingredients commonly used in cosmetic compositions in addition to the composition as an active ingredient, and may include, for example, conventional auxiliary agents and carriers such as stabilizers, solubilizers, vitamins, pigments, and fragrances.

[0039] In another embodiment, the composition may be a pharmaceutical composition.

[0040] The pharmaceutical composition may additionally comprise a pharmaceutically acceptable diluent or carrier. The diluent may be lactose, corn starch, soybean oil, microcrystalline cellulose, or mannitol, and the lubricant may be magnesium stearate, talc, or a combination thereof. The carrier may be an excipient, a disintegrant, a binder, a glidant, or a combination thereof. The excipient may be microcrystalline cellulose, lactose, low-substituted hydroxycellulose, or a combination thereof. The disintegrant may be calcium carboxymethylcellulose, sodium starch glycolate, calcium dihydrogen phosphate anhydrous, or a combination thereof. The binder may be polyvinylpyrrolidone, low-substituted hydroxypropylcellulose, hydroxypropylcellulose, or a combination thereof. The lubricant may be magnesium stearate, silicon dioxide, talc, or a combination thereof.

[0041] The pharmaceutical composition may be formulated as an oral or parenteral dosage form. The oral dosage form may be a granule, powder, liquid, tablet, capsule, dry syrup, or a combination thereof. The parenteral dosage form may be an injection.

[0042] In another specific example, the composition may be a composition for external use in the skin.

[0043] The above-mentioned external preparation for skin may be a cream, gel, ointment, skin emulsifier, skin suspension, transdermal patch, drug-containing bandage, lotion, or a combination thereof. The above-mentioned external preparation for skin may be appropriately mixed with ingredients commonly used in external preparations for skin such as cosmetics or medicines, such as aqueous ingredients, oily ingredients, powder ingredients, alcohols, moisturizers, thickeners, UV absorbers, whitening agents, preservatives, antioxidants, surfactants, fragrances, colorants, various skin nutrients, or a combination thereof, as needed. The above skin external preparation may also appropriately contain metal sequestrants such as disodium edetate, trisodium edetate, sodium citrate, sodium polyphosphate, sodium metaphosphate, and gluconic acid; caffeine, tannin, bellapamil, licorice extract, glabridin, hot water extract of the fruit of Kalin; various herbal medicines; drugs such as tocopherol acetate, glycyrrhizic acid, tranexamic acid and derivatives or salts thereof; vitamin C, magnesium ascorbic acid phosphate, ascorbic acid glucoside, arbutin, kojic acid, glucose, fructose, and trehalose; and the like.

[0044] In another specific example, the composition may be a health functional food composition.

[0045] The above health functional food composition can be used alone or in combination with other foods or food ingredients, and can be used appropriately according to a conventional method. The amount of the active ingredients mixed can be appropriately determined depending on the purpose of use (prevention, health, or therapeutic treatment). Generally, when manufacturing a food or beverage, the composition of the present specification can be added in an amount of 15 parts by weight or less based on the raw material. There is no particular limitation on the type of the health functional food. Among the types of health functional foods, the beverage composition can contain various flavoring agents or natural carbohydrates as additional ingredients, like conventional beverages. The natural carbohydrates include monosaccharides such as glucose and fructose, disaccharides such as maltose and sucrose, polysaccharides such as dextrin and cyclodextrin, and sugar alcohols such as xylitol, sorbitol, and erythritol. As a sweetener, a natural sweetener such as thaumatin and stevia extract, or a synthetic sweetener such as saccharin and aspartame can be used. The health food composition may also contain nutrients, vitamins, electrolytes, flavoring agents, coloring agents, pectic acid and its salts, alginic acid and its salts, organic acids, protective colloid thickeners, pH adjusters, stabilizers, preservatives, glycerin, alcohol, carbonating agents used in carbonated beverages, or combinations thereof. The health functional food composition may also contain fruit pulp for the production of natural fruit juice, fruit juice drinks, vegetable drinks, or combinations thereof.

[0046] Another aspect provides a method for preventing, improving, or treating a skin condition in a subject, comprising administering to the subject an effective amount of the composition or the organoid culture. The specific details of the skin condition are as described above.

[0047] As used herein, the terms "administering," "introducing," and "implanting" are used interchangeably and may refer to placement of a composition according to one embodiment into a subject by a method or route that results in at least partial localization of the composition to a desired site according to one embodiment.

[0048] Administration may be by any method known in the art. Administration may be administered directly to the subject by any means, including intravenous, intramuscular, oral, transdermal, mucosal, intranasal, intratracheal, or subcutaneous administration. Administration may be systemic or local.

[0049] The subject may be a mammal, such as a human, cow, horse, pig, dog, sheep, goat, or cat. The subject may be an object in need of skin beauty improvement, such as skin moisturization, skin barrier strengthening, or skin wrinkle improvement.

[0050] The above administration is 0.1 mg to 1,000 mg per day per individual of the composition according to one specific example, for example, 0.1 mg to 500 mg, 0.1 mg to 100 mg, 0.1 mg to 50 mg, 0.1 mg to 25 mg, 1 mg to 1,000 mg, 1 mg to 500 mg, 1 mg to 100 mg, 1 mg to 50 mg, 1 mg to 25 mg, 5 mg to 1,000 mg, 5 mg to 500 mg, 5 mg to 100 mg, 5 mg to 50 mg, 5 mg to 25 mg, 10 mg to 1,000 mg, 10 mg to 500 mg, 10 mg to It may be administered at 100 mg, 10 mg to 50 mg, or 10 mg to 25 mg. However, the dosage may be prescribed in various ways depending on factors such as the formulation method, administration method, patient's age, weight, sex, pathological condition, food, administration time, administration route, excretion rate, and reaction sensitivity, and a person skilled in the art can appropriately adjust the dosage by considering these factors. The number of administrations may be once a day or twice or more within the range of clinically acceptable side effects, and the administration site may be administered at one or more sites, and the total number of administration days may be from 1 to 30 days per treatment at intervals of 2 to 5 days daily or every day. If necessary, the same treatment may be repeated after an appropriate period. For animals other than humans, the same dosage per kg as for humans may be administered, or the dosage may be converted to the above dosage based on the volume ratio (e.g., average value) of the organs (heart, etc.) of the target animal and humans.

[0051] Another aspect provides a method for strengthening hair roots comprising administering an effective amount of the composition or the organoid culture to a subject in need thereof.

[0052] Another aspect provides a method for preventing hair loss or promoting hair growth, comprising administering an effective amount of the composition or the organoid culture to a subject in need thereof.

[0053] Another aspect provides the use of organoid cultures for the preparation of cosmetic compositions for improving skin condition.

[0054] Another aspect provides the use of organoid cultures for the preparation of health functional food compositions for improving skin conditions.

[0055] Another aspect provides the use of organoid cultures for the preparation of cosmetic compositions for strengthening hair roots.

[0056] Another aspect provides the use of an organoid culture for the preparation of a cosmetic composition for preventing hair loss or promoting hair growth.

[0057] Since the above method or use includes or utilizes the above-described composition or organoid culture solution as is, description of common contents between them is omitted to avoid excessive complexity of the present specification.

[0058] Organoid cultures according to the daily aspect have excellent anti-aging, moisturizing activity, and hair root strengthening effects, and can be usefully used to improve skin condition or scalp.

[0059] Figure 1 shows the effect of an organoid culture according to one specific example on the expression of AQP3.

[0060] Figure 2 shows the effect of an organoid culture medium according to one specific example on the expression of HAS3.

[0061] Figure 3 shows the effect of organoid culture according to one specific example on the expression of COL1A1.

[0062] Figure 4 shows the effect of an organoid culture medium according to one specific example on the expression of FBN.

[0063] Figure 5 shows the effect of an organoid culture medium according to one specific example on cell proliferation of mammary papilla cells.

[0064] Figure 6 shows the effect of an organoid culture medium according to one specific example on the expression of VEGF.

[0065] Figure 7 shows the effect of an organoid culture medium according to one specific example on the expression of FGF.

[0066] Hereinafter, preferred examples are presented to aid in understanding the present invention. However, the following examples are provided solely to facilitate a better understanding of the present invention, and the scope of the present invention is not limited by the following examples.

[0067]

[0068] [Example]

[0069] Example 1. Preparation of organoid culture medium (1)

[0070] Organoid culture medium was prepared according to the daily pattern. Specifically, organoid culture medium was prepared by mixing X100 GLUTAMATE 1, B-27 supplement, N2 supplement, 2-Mercaptoethanol, and Normocin with Advanced D-MEM / F-12 and Neurobasal medium.

[0071] Example 2. Preparation of organoid culture medium (2)

[0072] Organoid cultures were prepared according to the daily pattern. Specifically, hESC or iPSC cells were cultured in 500 μl of Example 1 in a 24-well plate, with the existing medium removed and replaced with fresh medium every three days, to prepare organoids. After culturing for 100 to 200 days, organoids that had developed hair were replaced with the Example 1 medium and cultured for an additional three days. Afterwards, the organoid cultures were harvested, and cells and debris were sedimented using a centrifuge, and only the supernatant was collected.

[0073]

[0074] [Experimental Example]

[0075] Experimental Example 1. Confirmation of improved skin moisturizing effect.

[0076] The skin moisturizing improvement effect of organoid culture according to the daily aspect was confirmed. Specifically, human epidermal cells (HaCaT) were cultured in DMEM (Dulbecco's modified Eagle's medium) containing 10% fetal bovine serum (FBS) and 1% penicillin, and the culture was performed for 24 hours in an incubator under the conditions of 37℃, 5% CO2, and 95% humidity. After removing the medium, DPBS was added, washed once, and replaced with medium without FBS. Then, Examples 1 and 2 were treated at various concentrations (10, 100, 1000, and 10000 ppm) and further cultured for 24 hours. Pure distilled water was used as a negative control, and a group with 1 μM retinoic acid was used as a positive control. Afterwards, cells from each medium were harvested, washed with chilled PBS, and RNA was isolated using an RNAprep kit (Monarch® Total RNAprep Kit, NEB, USA). RNA was quantified at 260 nm with a nanodrop, and 2 μg of RNA was used to synthesize cDNA in an amplifier (C1000 Thermal Cycler, Bio-Rad, USA). The synthesized cDNA was used as a template, and CyberGreen (SYBR Green supermix, Applied Biosystems, USA) was added together with primers and cDNA for the target genes AQP3 and HAS3, and real-time polymerase chain reaction was performed in a real-time PCR machine (Step One Plus, Applied Biosystems, USA). The real-time PCR reaction was performed by activating the polymerase at 94°C for 5 minutes, followed by 40 cycles of 95°C for 30 seconds, 60°C for 30 seconds, and 72°C for 30 seconds. The expression levels of AQP3 and HAS3 genes were finally analyzed through correction for the β-actin gene, and the results are shown in Figures 1 and 2.

[0077] Figure 1 shows the effect of an organoid culture according to one specific example on the expression of AQP3.

[0078] Figure 2 shows the effect of an organoid culture medium according to one specific example on the expression of HAS3.

[0079] As a result, as shown in FIGS. 1 and 2, it was confirmed that Example 2 increased the expression of AQP3 and HAS3 in a concentration-dependent manner. Specifically, it was confirmed that Example 2 significantly increased the expression of AQP3 compared to the positive control group at a concentration of 100 ppm or higher, and increased the expression of HAS3 compared to the positive control group at a concentration of 1000 ppm.

[0080] Therefore, the organoid culture according to one specific example is effective in moisturizing the skin by increasing the expression of AQP3 and HAS3.

[0081] Experimental Example 2. Confirmation of skin elasticity effect

[0082] The effects of organoid cultures on skin elasticity and wrinkle improvement according to the daily aspect were confirmed. Specifically, human dermal fibroblast cell line (Human dermal fibroblast, Hs68) was cultured in DMEM (Dulbecco's modified Eagle's medium) containing 10% fetal bovine serum (FBS) and 1% penicillin, and the culture was performed for 24 hours in an incubator under the conditions of 37℃, 5% CO2, and 95% humidity. After that, the medium was removed, DPBS was added, and 20 mJ / cm2 of UVB was irradiated or not. Immediately after UVB irradiation, DPBS was removed and replaced with medium without FBS, and then treated with Examples 1 and 2 at various concentrations (10, 100, 1000, and 10000 ppm) and further cultured for 24 hours. Pure distilled water was used as a negative control, and a group supplemented with 1 nM TGF-beta1 was used as a positive control. Afterwards, cells from each medium were harvested, washed with chilled PBS, and RNA was isolated using an RNAprep kit (Monarch® Total RNAprep Kit, NEB, USA). RNA was quantified at 260 nm with a nanodrop, and 2 μg of RNA was used to synthesize cDNA in an amplifier (C1000 Thermal Cycler, Bio-Rad, USA). The synthesized cDNA was used as a template, and CyberGreen (SYBR Green supermix, Applied Biosystems, USA) was added together with primers and cDNA for the target genes COL1A1 and FBN1, and real-time polymerase chain reaction was performed in a real-time PCR machine (Step One Plus, Applied Biosystems, USA). The real-time PCR reaction was performed by polymerase activation at 94°C for 5 minutes, followed by 40 cycles of 95°C for 30 seconds, 60°C for 30 seconds, and 72°C for 30 seconds.The expression levels of the COL1A1 and FBN1 genes were finally analyzed through correction for the β-actin gene, and the results are shown in Figures 3 and 4, respectively.

[0083] Figure 3 shows the effect of an organoid culture according to one specific example on the expression of COL1A1.

[0084] Figure 4 shows the effect of an organoid culture according to one specific example on the expression of FBN1.

[0085] As a result, as shown in FIGS. 3 and 4, it was confirmed that Examples 1 and 2 increased the expression of COL1A1 and FBN1 compared to the untreated control group.

[0086] Therefore, the organoid culture according to one specific example has an excellent effect on skin elasticity by increasing the expression of COL1A1 and FBN1, regardless of whether it is obtained before or after culturing the cells.

[0087] Experimental Example 3. Confirmation of Cell Proliferation

[0088] The cell proliferation effect of the organoid culture solution according to the daily aspect was confirmed. Specifically, 2x10 HFDPCs were seeded in a 96-well plate. 4After dividing into the number of cells, they were cultured in an incubator at 37°C and 5% CO2 for 24 hours. Afterwards, the medium was replaced with a medium containing nothing, and the organoid culture was treated and cultured for an additional 24 hours. Minoxidil was used as a positive control, and after 24 hours, the medium in each well was removed, washed once with Dulbecco's phosphate buffered saline (DPBS), and then replaced with a medium containing 500 μl of 0.5 mg / mL MTT tetrazolium (Sigma-aldrich, St. Louis, USA) per well, and reacted at 37°C for 4 hours. Afterwards, the absorbance was measured at 570 nm using Victor 3. 0.1 uM Minoxidil (MXD), known as a hair growth promoter, was set as a positive control, and the survival rate of cells treated with a medium containing nothing was set as a negative control, and the HFDPC proliferation ability was measured as a percentage of the average absorbance value.

[0089] Figure 5 shows the effect of an organoid culture medium according to one specific example on cell proliferation of mammary papilla cells.

[0090] As a result, as shown in Fig. 5, it was confirmed that Examples 1 and 2 promoted cell proliferation of breast papilla cells compared to the untreated control group. Specifically, it was confirmed that Examples 1 and 2 had a positive effect on cell proliferation of breast papilla cells similar to or greater than minoxidil.

[0091] Therefore, the organoid culture according to one specific example promotes cell proliferation of hair follicle cells, regardless of whether it is obtained before or after culturing the cells, and can be usefully used for promoting or improving hair growth or preventing, improving or treating hair loss.

[0092] Experimental Example 4. Confirmation of hair growth effect

[0093] The hair growth effect of organoid culture according to the daily pattern was confirmed. Specifically, human hair follicle duct cells (HFDPCs) were seeded in a 6-well plate at a density of 3x10 5 After dividing cells per well, they were cultured for 24 hours under cell culture conditions. After 24 hours, the medium was discarded, washed with PBS, and the cells were starved using medium that did not contain FBS. The next day, the medium was replaced with medium that did not contain FBS, 0.1 uM minoxidil, and the organoid culture medium of Example 1 or Example 2, and then cultured for an additional 24 hours. After this, RNA was isolated from the cells using the RNeasy Mini Kit (Qiagen), and RNA was quantified at 260 nm using a nanodrop. Then, cDNA was synthesized using an amplifier (C1000 Thermal Cycler, Bio-Rad, USA) using 2 μg of RNA each. The expression levels of the VEGF and FGF7 genes were finally evaluated by performing real-time polymerase chain reaction on a real-time PCR machine using a mixture of the synthesized cDNA, target proteins VEGF and FGF7, primers, and cyanine dye CyberGreen (SYBR Green supermis, Applied Biosystems, USA). The real-time PCR reaction was performed by activating the polymerase at 94°C for 5 minutes, followed by 40 cycles of 95°C for 30 seconds, 60°C for 30 seconds, and 72°C for 30 seconds. The expression levels of the VEGF and FGF genes were finally analyzed through correction for the β-actin gene, and the results are shown in Figs. 6 and 7, respectively.

[0094] Figure 6 shows the effect of an organoid culture medium according to one specific example on the expression of VEGF.

[0095] Figure 7 shows the effect of an organoid culture medium according to one specific example on the expression of FGF.

[0096]

[0097] The foregoing description of the present invention is provided for illustrative purposes only. Those skilled in the art will readily appreciate that the present invention can be readily modified into other specific forms without altering the technical spirit or essential characteristics of the present invention. Therefore, the embodiments described above should be understood as illustrative in all respects and not restrictive.

Claims

1. A cosmetic composition for improving skin condition containing an organoid culture solution.

2. A cosmetic composition for improving skin condition according to claim 1, wherein the organoid culture solution is obtained before culturing cells isolated from stem cells or organ-derived cells or after culturing the cells.

3. A cosmetic composition for improving skin condition according to claim 1, wherein the skin condition is anti-aging, wrinkle improvement, moisturizing improvement, skin barrier strengthening, or elasticity improvement.

4. A cosmetic composition for improving skin condition according to claim 3, wherein the aging is aging caused by ultraviolet rays.

5. A health functional food composition for improving skin condition containing an organoid culture solution.

6. A cosmetic composition for strengthening hair roots containing an organoid culture solution.

7. A cosmetic composition for preventing hair loss or promoting hair growth containing an organoid culture solution.

8. Use of organoid culture medium for the preparation of a cosmetic composition for improving skin condition.

9. Use of organoid culture medium for the manufacture of a health functional food composition for improving skin condition.

10. Use of organoid culture medium for preparing a cosmetic composition for strengthening hair roots.

11. Use of organoid culture medium for the manufacture of a cosmetic composition for preventing hair loss or promoting hair growth.

12. A method for improving a skin condition, comprising the step of administering an effective amount of an organoid culture solution to an individual in need thereof.

13. A method for strengthening hair roots, comprising the step of administering an effective amount of an organoid culture solution to an individual in need thereof.

14. A method for preventing hair loss or promoting hair growth, comprising a step of administering an effective amount of an organoid culture solution to an individual in need thereof.

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