Automatic loading device and biological tissue transfer and loading device

Abstract

Provided in the present application is an automatic loading device, comprising: one or more negative pressure ports and a negative pressure device. The one or more negative pressure ports are provided on one wall of the automatic loading device, and are connected to liquid or air to be suctioned via one or more cannulas. The negative pressure device fills the one or more cannulas with the liquid or air via the one or more negative pressure ports. The device improves the filling efficiency of cannulas and reduces the complexity of cannulas loading. The automatic loading device can be used for the transfer and loading of biological tissues, in which one or more carrying rods carrying biological tissues are held by clamps.

Description

Automated filling device and biological tissue transfer and filling device TECHNICAL FIELD

[0001] The utility model relates to biological engineering technical field especially, relate to an automated filling device and biological tissue transfer and filling device. BACKGROUND

[0002] Although the freezing technology, especially the vitrification freezing technology has achieved remarkable success in the field of human medicine and is widely used in reproductive medicine, its application in the livestock industry has been greatly limited. This limitation is mainly affected by factors such as cost, professional knowledge and ranch environment. Vitrification freezing requires expensive equipment and professional knowledge, which may be impractical for the livestock industry. Specifically, the embryo technician needs to manually fill one or more segments of culture solution into the straw, which requires meticulous training and a professional laboratory environment. In the ranch environment, there is usually a lack of personnel with these professional skills, so it is difficult to apply this technology.

[0003] In the prior art, embryo transfer requires skilled embryo technicians to operate, and the embryo needs to be transferred to the recipient cow as soon as possible after thawing. In the poor ranch environment, manually filling liquid in the straw not only slows down the speed, but also cannot realize the uniformity of filling capacity and filling position, and cannot guarantee aseptic operation, which will lead to a decrease in operation success rate. In addition, manual embryo transfer and straw filling operation is complicated, inefficient and prone to errors.

[0004] UTILITY MODEL CONTENTS

[0005] The main purpose of the utility model is to provide an automated filling device and biological tissue transfer and filling device to solve the problem of complex manual liquid filling operation in the straw and low efficiency in the prior art.

[0006] According to the embodiment of the utility model, an automated filling device is provided, which comprises: one or more negative pressure ports, the one or more negative pressure ports are arranged on one wall of the automated filling device, and the one or more negative pressure ports are connected with the liquid or air to be sucked through one or more cannulas; and a negative pressure device, which fills the liquid or air into the one or more cannulas through the one or more negative pressure ports.

[0007] Among them, each cannula includes multiple segments of liquid, and the multiple segments of liquid are separated by air.

[0008] Among them, at least one segment of liquid in the multiple segments of liquid contains biological tissue.

[0009] Among them, the biological tissue includes embryo or ovum.

[0010] The number of the multiple sections of liquid in each sleeve is equal.

[0011] The number of the one or more negative pressure ports is 1-3.

[0012] The negative pressure device is an injection pump, a peristaltic pump, a plunger pump, a diaphragm pump, a vacuum pump, a centrifugal pump, a vane pump or a piston pump.

[0013] The liquid is a culture solution, a transplantation solution or an operation solution.

[0014] The automatic filling device further comprises a heating table arranged on the upper surface of the automatic filling device, and the heating table comprises a surface for placing a dish container and a surface for placing a container containing liquid.

[0015] The automatic filling device further comprises a stirring device arranged below the container containing liquid, and the stirring device is a magnetic stirrer.

[0016] According to the embodiment of the utility model, the biological tissue transfer and filling device is further provided, it includes: automatic filling device, the automatic filling device includes one or more negative pressure ports and negative pressure device, one or more negative pressure ports are connected with one or more sleeves;Clamp, the clamp holds one or more load bars with biological tissue;Wherein, the automatic filling device, the clamp and the load bar are used in combination to complete the transfer and filling of biological tissue.

[0017] The biological tissue transfer and filling device comprises a filling state and a transfer state, when the biological tissue transfer device is in the filling state, the one or more negative pressure ports are connected with the liquid or air to be sucked through one or more sleeves, and the negative pressure device fills the liquid or air into the one or more sleeves through the one or more negative pressure ports;When the biological tissue transfer and filling device is in the transfer state, the end of the one or more load bars and the end of the one or more sleeves are opposite, the clamp drives the one or more load bars to move axially, so that the biological tissue carried in the one or more load bars is transferred to the corresponding one or more sleeves.

[0018] The biological tissue comprises an embryo or an ovum.

[0019] According to the technical scheme of the utility model, the one or more negative pressure ports are arranged, the liquid or air to be sucked is communicated with the negative pressure ports through the multiple sleeves, and the negative pressure device automatically fills the liquid or air to be sucked into the sleeves through the negative pressure ports, so that the filling efficiency of the sleeves is improved and the complexity of the filled sleeves is reduced without complex professional knowledge and fine operation of an embryo teacher. BRIEF DESCRIPTION OF DRAWINGS

[0020] Fig. 1 is a schematic diagram of an automated filling device according to embodiments of the present application;

[0021] Fig. 2 is a schematic diagram of the internal structure of an automated filling device according to embodiments of the present application;

[0022] Fig. 3A is a schematic diagram of a biological tissue transfer device according to embodiments of the present application;

[0023] Fig. 3B is an enlarged view of a portion of Fig. 3A. DETAILED DESCRIPTION

[0024] In order to make the objectives, technical solutions and advantages of the present application clearer, the technical solutions of the present application will be described below in detail with reference to specific embodiments of the present application and corresponding drawings. Obviously, the described embodiments are only some of the embodiments of the present application, rather than all the embodiments. Based on the embodiments in the present application, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the scope of protection of the present application.

[0025] The technical solutions provided by the embodiments of the present application will be described in detail below with reference to the drawings.

[0026] An automated filling device is provided according to embodiments of the present application, which has a closed housing. In combination with reference to Figs. 1 and 2, the automated filling device 10 includes one or more negative pressure ports 11 provided on one wall of the housing, a negative pressure device 12 provided in the housing, and one or more cannulas (not shown in the drawings). The one or more negative pressure ports 11 are provided on any wall of the housing, and the number of negative pressure ports 11 can be specifically 1-3. One end of the one or more cannulas is in communication with the negative pressure port 11, and the other end of the one or more cannulas is in communication with an external test tube or air, the external test tube carrying a liquid to be aspirated. The negative pressure device 12 is in communication with the negative pressure port 11, and the negative pressure device 12 aspirates the liquid or air in the external test tube by negative pressure and fills the liquid or air into the cannula to form one or more sections of liquid in the cannula. Specifically, the negative pressure device 12 can include at least one pump structure, which can be one or more of a syringe pump, a peristaltic pump, a plunger pump, a diaphragm pump, a vacuum pump, a centrifugal pump, a vane pump, or a piston pump.

[0027] In some embodiments of the present application, the number of negative pressure ports 11 is three, and therefore the negative pressure device 12 can include one or three pump structures. When the negative pressure device 12 includes one pump structure, the pump structure can be connected to the three negative pressure ports 11 through a multi-way joint. When the negative pressure device 12 includes three pump structures, the three pump structures are respectively connected to the three negative pressure ports 11. A support frame for supporting the multi-way joint can be further arranged below the multi-way joint. In other embodiments of the present application, the number of negative pressure ports 11 can also be one, and the negative pressure device 12 includes one pump structure. The negative pressure port 11 can be connected to the plurality of cannulas through a multi-way joint.

[0028] Referring to FIG. 1, the automated filling device 10 further includes a heating platform 13 arranged on the upper surface of the housing of the automated filling device 10. The heating platform 13 includes a horizontal surface 131 and a liquid container 132 (e.g., a beaker) for containing liquid. A heating device or heating sheet (not shown) is arranged below the heating platform 13. The heating device can heat liquid materials such as solutions required for embryo transfer and / or oil for thawing. The liquid materials can be placed on the surface 131 by a dish container and / or placed in the liquid container 132. A temperature sensor (not shown) can be arranged on the heating platform 13 to maintain a constant temperature of the heating platform for a long time. A stirring device 14, which can be a magnetic stirrer, can be arranged below the liquid container 132. The stirring device 14 can stir the liquid materials in the container while the heating device heats the liquid materials to avoid uneven heating of the liquid materials.

[0029] Further, an electric control device, which can be a circuit board, can be arranged in the automated filling device 10. The electric control device can adjust the suction speed of each section of liquid, the suction volume of each section of liquid, the heating temperature of the heating platform, and the rotation speed of the stirring device according to actual needs. A display screen for displaying the above parameters and control buttons for adjusting the above parameters can be arranged on the upper surface of the automated filling device 10.

[0030] In actual use, the automated filling device 10 is first connected to external test tubes through the cannulas. The automated filling device 10 sucks the liquid in the external test tubes into the cannulas to form a section of liquid in each cannula. Then, the automated filling device 10 is connected to gas through the cannulas. The automated filling device 10 sucks the gas and fills it into the cannulas to form a section of gas in each cannula. The above operations are repeated to interval fill multiple sections of liquid in the cannulas, and the sections of liquid are separated by gas, which can be air. Further, the number of sections of liquid in each cannula is equal, which can be three or five sections of liquid. At least one section of liquid in each cannula contains biological tissue.

[0031] According to the embodiment of the utility model, the automatic filling device and the clamp are electrically connected, and the biological tissue is transferred to the target animal body through the negative pressure device, so that the biological tissue is transferred to the target animal body in a simple and convenient manner. Specifically, in combination with FIGS. 3A and 3B, the biological tissue transfer and filling device comprises: an automatic filling device 10 and a clamp 20. Since the structure of the automatic filling device 10 has been described in detail above, it will not be described here again. The clamp 20 comprises: a first clamping part 21 and a second clamping part 22, and the first clamping part 21 can move axially relative to the second clamping part 22. One or more carrier rods 30 are clamped in the first clamping part 21, and one or more cannulas 40 are clamped in the second clamping part 22. The front end of the carrier rod 30 carries the biological tissue to be transferred, which can be an embryo or an ovum, etc. The negative pressure port 11 of the automatic filling device 10 is communicated with one end of the cannula 40 through a connecting pipe, and the automatic filling device 10 is also electrically connected with the clamp 20.

[0032] The biological tissue transfer device comprises a filling state and a transfer state. When the biological tissue transfer device is in the filling state, the other end of the cannula 40 is communicated with an external test tube or air, and the negative pressure device 12 fills liquid or air into the cannula 40 through the negative pressure port 11. When the biological tissue transfer device is in the transfer state, the other end of the cannula 40 is arranged opposite to the front end of the carrier rod 30. The automatic filling device 10 controls the axial movement of the first clamping part 21, and the first clamping part 21 drives the axial movement of the carrier rod 30, so that the front end of the carrier rod 30 is butted or contacted with the cannula 40. The biological tissue in the carrier rod 30 is transferred to a section of liquid in the cannula 40.

[0033] In actual use, first, 3 sections of liquid are filled in the cannula through the negative pressure device, and then the biological tissue is transferred from the front end of the carrier rod to the appropriate position in the cannula. Specifically, the biological tissue can be located in any section of liquid, so that at least one section of liquid in the cannula contains the biological tissue. Finally, 2 sections of liquid are filled in the cannula through the negative pressure device, so that 5 sections of liquid are formed in the cannula, so as to facilitate the subsequent transfer of the biological tissue to the target animal body.

[0034] According to the embodiment of the present application, by arranging the negative pressure device and the negative pressure port communicated with the negative pressure device, the negative pressure port is communicated with the liquid or air to be sucked through the cannula, and the negative pressure device automatically fills or fills the liquid to be sucked into the cannula, so that at least one section of liquid is formed in the cannula. Using the device does not require complex professional knowledge and delicate operation, effectively improves the filling efficiency of the cannula and reduces the operation complexity of the filled cannula. Further, by arranging the heating device and the stirring device in the automatic filling device, other operation devices required for biological tissue transfer are not required to be prepared additionally, and the working efficiency is further improved.

[0035] While the present disclosure has been described in detail with respect to particular embodiments, it will be understood that various changes and modifications can be made therein without departing from the spirit and scope of the embodiments. Accordingly, it is intended that the present application cover all such modifications and changes as fall within the scope of the application.

Claims

1. An automated filling device, characterized in that, The device comprises: one or more negative pressure ports arranged on a wall of the automated filling device, the one or more negative pressure ports being connected to the liquid or air to be sucked through one or more cannulas; a negative pressure device for filling the liquid or air into the one or more cannulas through the one or more negative pressure ports.

2. The automated filling device of claim 1, wherein, Each cannula contains multiple sections of liquid, and the multiple sections of liquid are separated by air.

3. The automated filling device of claim 2, wherein, At least one of the multiple sections of liquid contains biological tissue.

4. The automated filling device of claim 3, wherein, The biological tissue includes an embryo or an ovum.

5. The automated filling device of claim 1, wherein, Each cannula contains multiple sections of liquid, and the number of the multiple sections of liquid in each cannula is equal.

6. The automated filling device of claim 1, wherein, The number of the one or more negative pressure ports is 1-3.

7. The automated filling device of claim 1, wherein, The negative pressure device is an injection pump, a peristaltic pump, a plunger pump, a diaphragm pump, a vacuum pump, a centrifugal pump, a vane pump, or a piston pump.

8. The automated filling device of claim 1, wherein, The liquid is a culture solution, a transplantation solution, or an operation solution.

9. The automated filling apparatus of claim 1, wherein, The device further comprises: a heating table arranged on the upper surface of the automated filling device, the heating table comprising a surface for placing a dish container and a surface for placing a container containing liquid.

10. The automated filling apparatus of claim 9, wherein, The device further comprises: a stirring device arranged below the container containing liquid, the stirring device being a magnetic stirrer.

11. A biological tissue transfer and packing device, characterized by, The device comprises: an automated filling device comprising one or more negative pressure ports and a negative pressure device, the one or more negative pressure ports being connected to one or more cannulas; a clamp for clamping one or more carrier rods carrying biological tissue; wherein the automated filling device, the clamp, and the carrier rod are used in combination to complete the transfer and filling of biological tissue.

12. The biological tissue transfer and packing device according to claim 11, wherein, The biological tissue transfer and filling device comprises a filling state and a transfer state. When the biological tissue transfer and filling device is in the filling state, the one or more negative pressure ports are connected to the liquid or air to be sucked through one or more cannulas, and the negative pressure device fills the liquid or air into the one or more cannulas through the one or more negative pressure ports. When the biological tissue transfer and filling device is in the transfer state, the ends of the one or more carrier rods and the ends of the one or more cannulas are opposite to each other, and the clamp drives the one or more carrier rods to move axially, so that the biological tissue carried in the one or more carrier rods is transferred into the corresponding one or more cannulas.

13. The biological tissue transfer and packing device of claim 11, wherein, The biological tissue includes an embryo or an ovum.

Images