Compositions and methods for enriching photoreceptors
By employing JAM-B, CD26, and CD133 binding agents to enrich photoreceptor cells from mixed populations, the method addresses yield and purity challenges, achieving high-purity photoreceptor cell grafts for treating ocular disorders.
Patent Information
- Application Number
- PCT/EP2025/082614
- Authority / Receiving Office
- WO · WO
- Patent Type
- Applications
- Current Assignee / Owner
- Priority Date
- 2024-11-11
- Filing Date
- 2025-11-11
- Publication Date
- 2026-05-15
AI Technical Summary
Current methods for producing functional photoreceptor cells, particularly cone photoreceptors, from stem cell-derived retinal organoids face challenges in yield and purity, and the isolation of photoreceptors from mixed cell populations is hindered by the presence of non-photoreceptor cells, which can affect graft survival and integration.
The use of JAM-B, CD26, and CD133 binding agents, such as antibodies or nanobodies, to enrich photoreceptor and photoreceptor precursor cells from mixed populations through techniques like FACS and MACS, allowing for the separation of these cells based on specific cell surface markers.
This method achieves a significant enrichment of photoreceptor cells, with populations comprising at least 80-95% purity, enhancing the viability and integration potential of grafts for treating ocular disorders like IRDs, AMD, and GA.