Oligonucleotides, kits, and methods for amplifying a signal

The use of a signal amplifier template (SAT) with invader and insulator domains in a kit enhances nucleic acid detection by improving signal amplification and sensitivity, addressing the limitations of existing methods with complex instrumentation and low signal sensitivity.

WO2026112548A1PCT designated stage Publication Date: 2026-05-28SIGNAL BIOSCIENCE INC
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Patent Information

Authority / Receiving Office
WO · WO
Patent Type
Applications
Current Assignee / Owner
Filing Date
2025-11-22
Publication Date
2026-05-28

AI Technical Summary

Technical Problem

Existing nucleic acid detection methods, such as real-time PCR and Invader assays, face challenges with low signal sensitivity, high costs, and complex instrumentation due to low hybridization efficiency, background signals, and variable 3' base complications in signal oligonucleotides, necessitating improved methods for faster and more reliable detection.

Method used

A kit and method using a signal amplifier template (SAT) with invader and insulator domains, along with a reporter probe, to generate and amplify signal oligonucleotides, enhancing signal-to-noise ratio and simplifying detection protocols.

Benefits of technology

The method significantly improves real-time and digital PCR performance by increasing signal amplification, offering faster, more sensitive, and cost-effective nucleic acid detection with simplified instrumentation.

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Abstract

Provided herein are methods, uses, compositions, and kits that can include signal amplifier templates, invader domains, insulator domains, reporter probes, amplification probes, signal controllers, and / or signal converters. Such methods, uses, compositions, and kits can be used, for example, to amplify one or more signals, detect one or more signal oligonucleotides, and / or detect one or more target nucleic acids.
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Citation Information

Patent Citations

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    US4683202A

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