Method for constructing brain glioma organoids capable of preserving immune microenvironment
By using a specific ratio of culture medium and matrix gel-culture medium mixture, combined with a recombinant basement membrane, a glioma organoid model capable of preserving immune cells for a long period of time was constructed. This solves the problem of the inability to effectively preserve the tumor immune microenvironment in existing technologies, and enables more efficient research on cell activity and the tumor microenvironment.
WO2026114065A1PCT designated stage Publication Date: 2026-06-04BEIJING NEUROSURGICAL INST +1
Patent Information
- Authority / Receiving Office
- WO · WO
- Patent Type
- Applications
- Current Assignee / Owner
- BEIJING NEUROSURGICAL INST
- Filing Date
- 2025-11-19
- Publication Date
- 2026-06-04
Smart Images
Figure CN2025136156_04062026_PF_FP_ABST
Abstract
The present invention provides a method for constructing brain glioma organoids capable of preserving an immune microenvironment. The culture medium, culture system and method provided by the present invention overcome the shortages that, with the prolonging of culture time, a large number of tumor immune cells are lost and the tumor immune microenvironment is greatly damaged in current glioblastoma organoid models, and can more completely preserve the tumor microenvironment of glioblastoma for a longer time, and greatly retain the heterogeneity of organoid tissue. The present invention is suitable for constructing a new generation of glioblastoma organoid models, and provides more reliable research tools for tumor microenvironment research of glioblastoma, tumorigenesis mechanism research, drug efficacy screening, and the development and application of disease models.
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