Production of a compound comprising n-acetylglucosamine-beta-1,6-galactose-beta-1,4-glucose or n-acetylglucosamine-beta-1,6-galactose-beta-1,4-n-acetylglucosamine
The method of using UDP-GlcNAc and beta-1,6-N-acetylglucosaminyltransferase to form beta-1,6-glycosidic linkages addresses the challenge of producing N-acetylglucosamine-beta-1,6-galactose-beta-1,4-Zi-Z2 compounds efficiently, achieving high yields suitable for industrial use.
Patent Information
- Application Number
- PCT/EP2025/084717
- Authority / Receiving Office
- WO · WO
- Patent Type
- Applications
- Current Assignee / Owner
- Priority Date
- 2024-11-29
- Filing Date
- 2025-11-28
- Publication Date
- 2026-06-04
AI Technical Summary
The challenge lies in efficiently producing a compound comprising N-acetylglucosamine-beta-1,6-galactose-beta-1,4-Zi-Z2, where Zi is glucose or N-acetylglucosamine, and Z2 is absent or a saccharide, particularly due to the difficulty in introducing a beta-1,6-branch on growing saccharides or saccharides bound to peptides, proteins, or lipids.
A method involving the use of UDP-N-acetylglucosamine (UDP-GlcNAc) and a beta-1,6-N-acetylglucosaminyltransferase to transfer N-acetylglucosamine to a galactose-beta-1,4-Zi-Z2 acceptor, forming a beta-1,6-glycosidic linkage, optionally with additional glycosyltransferases, in controlled conditions such as temperature and pH, to produce the desired compound.
This method enables the production of the compound in high yields, reaching up to 100 g/L, suitable for industrial applications, using cells like Escherichia coli expressing the beta-1,6-N-acetylglucosaminyltransferase.