Cell culture scaffold material, production method for cell culture scaffold material, cell-cultured food product, cell culture substrate, and cell culture method

Abstract

Provided is a cell culture scaffold material that can be suitably used for a cell-cultured food product and can improve adhesion between cells and the surface of a culture substrate. The cell culture scaffold material contains an alkali extract from coffee seeds. The method for producing the cell culture scaffold material comprises subjecting coffee seeds to alkali extraction, neutralizing the obtained alkali extract with an acid, and drying the neutralized product. Preferably, the alkali extract is brought into contact with the surface of the cell culture substrate and then neutralized with an acid to adsorb or fix the coffee seed-derived protein contained in the alkali extract to the surface of the cell culture substrate.

Claims

1. A cell culture scaffold material characterized by containing an alkaline extract of coffee seeds.

2. The cell culture scaffold material according to claim 1, characterized in that the coffee seeds are the residue after hot water extraction treatment of coffee seeds.

3. A method for producing a cell culture scaffold, characterized by subjecting coffee seeds to alkaline extraction, neutralizing the resulting alkaline extract with acid, and drying it.

4. A method for producing a cell culture scaffold according to claim 3, characterized in that the alkaline extract is brought into contact with the surface of the cell culture substrate, and then neutralized with an acid to adsorb or fix the coffee seed-derived protein contained in the alkaline extract onto the surface of the cell culture substrate.

5. The method for producing a cell culture scaffold according to claim 3 or 4, characterized in that the coffee seeds are the residue after hot water extraction treatment of coffee seeds.

6. A cell culture food characterized by comprising the cell culture scaffold material according to claim 1 or 2 and cultured cells.

7. A cell culture substrate characterized in that the cell culture scaffold material described in claim 1 or 2 is adsorbed or fixed to the surface.

8. A cell culture method characterized by culturing cells using the cell culture scaffold material described in claim 1 or 2, or the cell culture substrate described in claim 7.

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