Yeast nucleic acid detection

Abstract

Nucleic acid primers and probes are provided for nucleic acid sequence amplification and detection of yeast target nucleic acids. The target nucleic acids include a Nakaseomyces glabratus target nucleic acid, a Pichia kudriavzevii target nucleic acid, a Candida tropicalis target nucleic acid, a Candida albicans target nucleic acid, a 5 Candida parapsilosis target nucleic acid and / or a Candida dubliniensis target nucleic acid. Also provided are methods of detecting, preventing and / or treating yeast treating or preventing yeast colonization, infection or infestation.

Description

[0001] TITLE

[0002] YEAST NUCLEIC ACID DETECTION TECHNICAL FIELD THIS INVENTION relates to nucleic acid sequence amplification. More particularly, this invention relates to nucleic acid sequence amplification and detection of yeast nucleic acids.

[0003] BACKGROUND

[0004] Yeast are single-celled microorganisms of Kingdom Fungi, which contain many important pathogens that cause candidiasis. The yeast species most commonly associated with invasive candidiasis include Nakaseomyces glabratus [formerly Candida glabrata), Pichia kudriavzevii [formerly Candida krusei]), Candida albicans, Candida parapsilosis and Candida tropicalis, which are all listed on the WHO's inaugural fungal priority pathogens list and contribute to 50-90% of all cases of candidiasis in humans.

[0005] N. glabratus (formerly C. glabrata) is a haploid yeast that is a commensal of human mucosal tissues, but can cause intractable invasive infections, particularly in people with underlying risk factors such as immunodeficiency, diabetes, or those who have received broad-spectrum antibiotics or chemotherapy. N. glabratus is often the second or third most common cause of candidiasis as an opportunistic pathogen. N. glabratus infections can affect the urogenital tractor even cause systemic infections by entrance of the fungal cells in the bloodstream (Candidemia), especially prevalent in immunocompromised patients. Infection is associated with high mortality rates and resistance to azole and echinocandin drugs is common, although N. glabratus remains mostly susceptible to amphotericin and flucytosine. However, the incidence of the disease and antifungal resistance is increasing, with these increases associated with the use of antifungal agents.

[0006] P. kudriavzevii (formerly Candida krusei) is a budding yeast that historically has been used as a fermentative yeast and de-bittering agent in chocolate production. However, P. kudriavzevii has more recently emerged as a fungal nosocomial pathogen in the immunocompromised and those with hematological malignancies. P. kudriavzevii has natural resistance to fluconazole, a common antifungal agent, and is often found in patients who have had prior fluconazole exposure, thus making prophylactic fluconazole use potentially problematic. Mortality due to P. kudriavzevii is much higher than the more common C. albicans.

[0007] C. albicans is an opportunistic pathogenic yeast that is a common member of the human gut flora, but can become pathogenic, particularly in immunocompromised individuals. Candidiasis is, for example, often observed in HIV-infected patients. C. albicans is also the most common fungal species isolated from biofilms. There are relatively few drugs that can successfully treat C. albicans-associated candidiasis, but some common treatments include amphotericin B, echinocandin, or fluconazole for systemic infections, nystatin for oral and oesophageal infections, and clotrimazole for skin and genital yeast infections.

[0008] C. tropicalis is a common yeast pathogen in neutropenic hosts, in whom it may spread through the bloodstream to peripheral organs, and is also common in people with chronic lung diseases and leukaemia. This fungus has a high mortality rate in candidiasis cases (up to 60% in adults and 40% in children), and is associated with high rates of triazole resistance. Treatment options for invasive disease cases include amphotericin B, echinocandins, or extended-spectrum triazole antifungals, due to currently low reported resistance rates.

[0009] C. parapsilosis has become a significant cause of sepsis and of wound and tissue infections in immunocompromised humans. Unlike C. albicans and C. tropicalis, C. parapsilosis is not an obligate human pathogen, having been isolated from non-human sources such as domestic animals, insects and soil. C. parapsilosis is also a normal human commensal and is one of the most common fungi isolated from human hands. Immunocompromised individuals and surgical patients, particularly those undergoing surgery of the gastrointestinal tract, are at high risk for infection with C. parapsilosis. Amphotericin B and fluconazole are often used in the treatment of C. parapsilosis infection.

[0010] Although not listed as a WHO fungal priority pathogen, Candida dubliniensis can cause rare cases of pneumonia and pleural infections that present with diverse, non-specific symptoms and can be rapidly fatal. Like other fungi, C. dubliniensis has a propensity for causing, or being associated with, opportunistic infections in people with immunosuppression of who are critically ill. This yeast is also the most common cause of oropharyngeal candidiasis in people with HIV, and has a high affinity for the cystic fibrosis airways, where it has been associated with declining lung function. C. dubliniensis commonly co-occurs with C. albicans, but these two species are impossible to differentiate based on phenotype alone. This phenotypic similarity suggests that numerous C. dubliniensis infections may have been misclassified as C. albicans, leading to underreporting of this opportunistic pathogen. Molecular methods that can accurately differentiate these two species thus has important clinical and epidemiological utility.

[0011] SUMMARY

[0012] The present invention has at least partly arisen from a recognition of the need to provide improved detection of pathogenic yeasts. Accordingly, the invention is broadly directed to detection of Nakaseomyces glabratus, Pichia kudriavzevii, Candida tropicalis, Candida albicans, Candida pcirapsilosis and / or Candida dubliniensis by nucleic acid sequence amplification. Detection may also include hybridization with respective probes specific for Nakaseomyces glabratus, Pichia kudriavzevii, Candida tropicalis, Candida albicans, Candida parapsilosis and / or Candida dubliniensis.

[0013] An aspect of the invention provides an isolated nucleic acid comprising, consisting essentially of, or consisting of a nucleotide sequence selected from the group consisting of:

[0014] (i) 5'-TTTCCTCGATTGTAAGTCAGGGAATC-3' (SEQ ID NO:1)

[0015] (ii) 5'-TTCCAGCAAAATGTATAGCCCAGTC -3' (SEQ ID NO:2)

[0016] (iii) 5'-AAGGATCTTCATTTCCTAGAGAGAGTATATTT-3' (SEQ ID NO:3) (iv) 5'-AAGGATCTTCATTTCCTAGAGAGAGTA-3' (SEQ ID NO:4)

[0017] (v) 5'-AGCAGAATGTCTTGCGTATAGTGAA-3' (SEQ ID NO:5)

[0018] (vi) 5'-CCTCTAATACAACCACCAACCGT-3' (SEQ ID NO:6)

[0019] (vii) 5'-ACCAGTAAACACACCACCATCTG-3' (SEQ ID NO:7)

[0020] (viii) 5'-CACCAGTAAACACACCACCATCT-3' (SEQ ID NO:8)

[0021] (ix) 5'-GCATTATTACTCCCATTTGAGGAACG-3' (SEQ ID NO:9)

[0022] (x) 5'-TTAGGATTTGCCACAAGGAACACTA-3' (SEQ ID NO:10) (xi) 5'-TGAATTTATCCACATCATCATAGCACC-3' (SEQ ID NO:11)

[0023] (xii) 5'-TCTTGCTAGTACCATTTGTCCAATACT-3' (SEQ ID NO:12)

[0024] (xiii) 5'-GAGTTGTTGGGAATTGTGGTCGA-3' (SEQ ID NO:13)

[0025] (xiv) 5'-CAACCTCAATCATCTCAAAAGCACA-3' (SEQ ID NO: 14)

[0026] (xv) 5'-GGTAGACCACTAATAGGTTGTATTCCA-3' (SEQ ID NO:15)

[0027] (xvi) 5'-TTTGTGCCCGTTATTGTATGCTAAG-3' (SEQ ID NO:16)

[0028] (xvii) 5'-TGTCAAACAGTGTCAACTGGATACA-3' (SEQ ID NO:17)

[0029] (xviii) 5'-TTTGTCAAACAGTGTCAACTGGATACA-3' (SEQ ID NO:18)

[0030] (xix) 5'-GCTCCTCATGCCATTACATTTACAG-3' (SEQ ID NO:19)

[0031] (xx) 5'-TGTTTGCTGGAGTTGTGCCAG-3' (SEQ ID NO:20)

[0032] (xxi) 5'-AGTCATACTACAAGCCTGTCAACC-3' (SEQ ID NO:21)

[0033] (xxil) 5'-AGTCATACTACAAGMCTGTCAACC-3' (SEQ ID NO:22)

[0034] (xxiii) 5'- TTTCCTCGATTGTAAGTCAGGGAATCCTGTGAAGGATCTTCATTTCCTAGA GAGAGTATATTTAGACTGGGCTATACATTTTGCTGGAA-3' (SEQ ID NO:23)

[0035] (xxiv) 5'- AGCAGAATGTCTTGCGTATAGTGAACACCAGTAAACACACCACCATCTGC GAGCTCTCGTACGAGTCTCCCATTATAGACGTGTCCATGAATAGGAAACG GTTGGTGGTTGTATTAGAGG-3' (SEQ ID NO:24)

[0036] (xxv) 5'- GCATTATTACTCCCATTTGAGGAACGGCGGGCCAACCATCTGCTGAATTT ATCCACATCATCATAGCACCATTGTTAATGAAAAATTCAACACATAGTGTT CCTTGTGGCAAATCCTAA-3' (SEQ ID NO:25)

[0037] (xxvi) 5'- TCTWGCTAGTACCATTTGTCCAATACTTTATAAAGTTGTTGTTTCATTCAA ACCCAACCTCAATCATCTCAAAAGCACATTTTTTTGAGATTTCGACCACAA TTSCCAACAACTC-3' (SEQ ID NO:26)

[0038] (xxvii) 5'- GGTAGACCACTAATAGGTTGTATTCCAAGTTTCAAAAGTATCAAGTGGAT CTGTTTGTCAAACAGTGTCAACTGGATACACACATTGTAGCAAGGCCCTG CAATAAGACCCAATCATTATGATCTGCCTGCAATACAAAATTCCTTAGCAT ACAATAACGGGCACAAA-3' (SEQ ID NO:27)

[0039] (xxviii) 5'- GCTCCTCATGCCATTACATTTACAGATAGTCATACTACAAGMCTGTCAACC CCATATGAAAAAAAAACTTCTTACAAACCAGTTCACGTTGCAACTGGCAC AACTCCAGCAAACA-3' (SEQ ID NO:28)

[0040] (xxix) a nucleotide sequence complementary to any of the nucleotide sequences in (i)-(xxviii);

[0041] (xxx) a nucleotide sequence having at least 70% nucleotide sequence to any of the nucleotide sequences in (i)-(xxix); and

[0042] (xxxi) a fragment of any one of the isolated nucleic acids of (i)-(xxx).

[0043] Suitably, the isolated nucleic acids comprising nucleotide sequences according to SEQ ID NOS: 1, 2, 5, 6, 9, 10, 12, 13, 15, 16, 19 and 20 are primers for nucleic acid sequence amplification.

[0044] Suitably, the isolated nucleic acids comprising the nucleotide sequences of SEQ ID NOS: 1 and 2 are respective forward and reverse primers suitable for nucleic acid sequence amplification of a Nakaseomyces glabratus target nucleic acid. Preferably, nucleic acid sequence amplification produces an 87 bp amplification product (SEQ ID NO:23).

[0045] Suitably, the isolated nucleic acids comprising the nucleotide sequences of SEQ ID NOS: 5 and 6 are respective forward and reverse primers suitable for nucleic acid sequence amplification of a Pichia kudriavzevii target nucleic acid. Preferably, nucleic acid sequence amplification produces a 120 bp amplification product (SEQ ID NO:24).

[0046] Suitably, the isolated nucleic acids comprising the nucleotide sequences of SEQ ID NOS: 9 and 10 are respective forward and reverse primers suitable for nucleic acid sequence amplification of a Candida tropicalis target nucleic acid. Preferably, nucleic acid sequence amplification produces a 119bp amplification product (SEQ ID NO:25).

[0047] Suitably, the isolated nucleic acids comprising the nucleotide sequences of SEQ ID NOS:12 and 13 are respective forward and reverse primers suitable for nucleic acid sequence amplification of a Candida albicans target nucleic acid. Preferably, nucleic acid sequence amplification produces a 115bp amplification product (SEQ ID NO:26).

[0048] Suitably, the isolated nucleic acids comprising the nucleotide sequences of SEQ ID NOS: 15 and 16 are respective forward and reverse primers suitable for nucleic acid sequence amplification of a Candida parapsilosis target nucleic acid. Preferably, nucleic acid sequence amplification produces a 168bp amplification product (SEQ ID NO:25).

[0049] Suitably, the isolated nucleic acids comprising the nucleotide sequences of SEQ ID NOS: 19 and 2.0 are respective forward and reverse primers suitable for nucleic acid sequence amplification of a Candida dubliniensis target nucleic acid. Preferably, nucleic acid sequence amplification produces a 115bp amplification product (SEQ ID NO:28).

[0050] According to the foregoing, the N. glabratus target nucleic acid encodes the Sip3p transcription cofactor in ATCC 2001 chr J (GVI51_J07799, 822329..822600); the P. kudriavzevii target nucleic acid encodes Chain A, Autophagy-related protein 18 in CY902 chromosome I (unannotated, 1184190..1184309); the C. tropicalis target nucleic acid is an unannotated region in MYA-3404 chr 3 (CP047871.1, 1205838..1205956); the C. albicans target nucleic acid is an unannotated region in SC5314 chr 1 (CP017623.1, 1562242..1562356); the C. parapsilosis target nucleic acid is CDC317 annotated contig 005504 (HE605202.1, 1028..1195) uncharacterised locus; and the C. dubliniensis target nucleic acid is CD36 chr 1 (FM992688.1, 1596149..1596263) uncharacterised locus.

[0051] Suitably, the isolated nucleic acids comprising nucleotide sequences according to SEQ ID NOS: 3, 4, 7, 8, 11, 14, 17, 18,21 and 22. are probes.

[0052] A probe comprising the nucleotide sequence set forth in SEQ ID NOS:3 and / or 4 is capable of hybridizing to an amplification product produced by the primers according to SEQ ID NOS:1 and 2.

[0053] A probe comprising the nucleotide sequence set forth in SEQ ID NO:7 and / or 8 is capable of hybridizing to an amplification product produced by the primers according to SEQ ID NOS:5 and 6. A probe comprising the nucleotide sequence set forth in SEQ ID NO:11 is capable of hybridizing to an amplification product produced by the primers according to SEQ ID NOS:9 and 10.

[0054] A probe comprising the nucleotide sequence set forth in SEQ ID NO:14 is capable of hybridizing to an amplification product produced by the primers according to SEQ ID NOS:12 and 13.

[0055] A probe comprising the nucleotide sequence set forth in SEQ ID NO:17 or SEQ ID NO:18 is capable of hybridizing to an amplification product produced by the primers according to SEQ ID NOS:15 and 16.

[0056] A probe comprising the nucleotide sequence set forth in SEQ ID NO:21 and / or SEQ ID NO:22 is capable of hybridizing to an amplification product produced by the primers according to SEQ ID NOS:19 and 20.

[0057] Suitably, the probes of SEQ ID NOS:3 and 4 are respectively capable of hybridizing to the amplification products of SEQ IS NO:23; the probes of SEQ ID NOS:7 and 8 are respectively capable of hybridizing to the amplification product of SEQ ID NO:24; the probe of SEQ ID NO: 11 is capable of hybridizing to the amplification product of SEQ IS NO:25 the probe of SEQ ID NO:14 is capable of hybridizing to the amplification product of SEQ IS NO:26; the probes of SEQ ID NOS:17 and 18 are respectively capable of hybridizing to the amplification product of SEQ ID NO:27' and / or the probes of SEQ ID NOS:21 and 22 are respectively capable of hybridizing to the amplification product of SEQ ID NO:28.

[0058] In an embodiment, the probes of SEQ ID NOS:3, 4, 7, 8, 11, 14, 17, 18, 21 and 22 are labelled, preferably with one or more fluorophores and / or quenchers. Nonlimiting examples include:

[0059] (A) SEQ ID NO:3 - 5'-FAM or 5'-CAL Fluor Gold 540 and 3'-BHQ-l;

[0060] (B) SEQ ID NO:4 - 5'-FAM and 3'-BHQ-1;

[0061] (C) SEQ ID NO:7 - 5'-FAM and 3'-BHQ-1;

[0062] ( D) SEQ ID NO:8 - 5'-FAM and 3'-BHQ-l;

[0063] (E) SEQ ID NO:11 - 5'-FAM and 3'-BHQ-1;

[0064] (F) SEQ ID NO:14 ■ S'-CAL Fluor Gold 540 and 3'-BHQ-l or 5' -CAL Fluor Red 610 and 3'-BHQ-2;

[0065] (G) SEQ ID NO:17 - 5'-FAM and 3'-BHQ-1; (H) SEQ ID NO:18 - 5'-FAM and 3'-BHQ-1;

[0066] (I) SEQ ID NO:21 - 5'-CAL Fluor Gold 540 and 3'-BHQ-l or Quasar 670 and 3'- BHQ-2 and;

[0067] (J) SEQ ID NO22 ■ 5'-HEX and 3'-BHQ-l.

[0068] In embodiments where SEQ ID NOS 1 and 2 are used in "singleplex" assays, 5'-FAM is a preferred label.

[0069] Another aspect of the invention provides a method of amplifying a yeast target nucleic acid with one or more primers according to the aforementioned aspect, or comprising a nucleotide sequence at least 70% identical thereto or a fragment thereof, to thereby produce an amplification product.

[0070] A further aspect of the invention provides a method of detecting the presence of absence of a yeast nucleic acid, said method including the step of subjecting a nucleic acid sample to nucleic acid sequence amplification with one or more primers according to the aforementioned aspect, or comprising a nucleotide sequence at least 70% identical thereto or a fragment thereof, wherein the presence of an amplification product indicates that the yeast nucleic acid is present in said sample and an absence of an amplification product indicates that the yeast nucleic acid is absent from said sample.

[0071] A related aspect of the invention provides a method of detecting the presence of absence of a yeast target nucleic acid, said method including the step of subjecting a nucleic acid sample to nucleic acid sequence amplification with primers to thereby produce one or more amplification products that respectively comprise, consist essentially of, or consist of a nucleotide sequence having at least 70% nucleotide sequence identity to any one of SEQ ID NOS:23-28, or a fragment thereof, wherein the presence of an amplification product indicates that the yeast target nucleic acid is present in said sample and an absence of an amplification product indicates that the yeast target nucleic acid is absent from said sample.

[0072] Suitably, the yeast is Nakaseomyces glabratus, Pichia kudriavzevii, Candida tropicalis, Candida albicans, Candida parapsiiosis and / or Candida dubliniensis.

[0073] The aforementioned nucleic acid sequence amplification methods may be performed with one, two, three, four, five or six primer pairs in a single nucleic acid sequence amplification reaction, referred to herein as "singleplex", "duplex" or "multiplex" reactions. Preferably, the methods are performed as singleplex or duplex reactions.

[0074] In an embodiment, the methods of the aforementioned aspects further include the step of hybridizing one or more probes to the amplification product(s) to facilitate detection of the amplification product(s). Preferably, the probes comprise nucleotide sequences set forth in any one of SEQ ID NOS: 3, 4, 7, 8, 11, 1417, 18, 21 and / or 22 as hereinbefore described. Preferably, the probes are labelled with a fluorophore and a quencher as hereinbefore described according to the first-mentioned aspect.

[0075] In particular embodiments, according to the methods of the aforementioned aspects, the one or more amplification products comprise respective nucleotide sequences selected from the group consisting of SEQ ID NOS:19-24, a nucleotide sequence at least 70% identical thereto or a fragment of these.

[0076] A still further aspect of the invention provides a method of treating or preventing yeast colonization, infection or infestation, said method including the steps of detecting the presence of a yeast nucleic acid according to the method of any of the aforementioned aspects and; treating yeast infection or infestation to thereby at least partly prevent, remove, eliminate or otherwise alleviate the yeast colonization, infection or infestation.

[0077] Suitably, the yeast is Nakaseomyces glabratus, Pichia kudriavzevii, Candida tropicalis, Candida albicans, Candida parapsi osis and / or Candida dubliniensis.

[0078] Another further aspect of the invention provides a method of determining the ability of one or more primers to amplify a nucleic acid, said method including the step of comparing the respective nucleotide sequences of one or more primers according to the first-mentioned aspect, with a nucleotide sequence of the nucleic acid to thereby determine the ability of the one or more primers to amplify the nucleic acid.

[0079] Preferably, the step of comparing the respective nucleotide sequences of the one or more primers with the nucleotide sequence of the nucleic acid is performed in silico. A yet still further aspect of the invention provides a kit comprising one or more isolated nucleic acid primers or probes respectively comprising nucleotide sequences according to SEQ ID NOS:1-22, or respectively comprising nucleotide sequences at least 70% identical thereto or a fragment thereof, and optionally one or more other components selected from the group consisting of:

[0080] (a) a thermostable DNA polymerase

[0081] (b) a reverse transcriptase; and

[0082] (c) one or more detection reagents.

[0083] Preferably, the probe(s) of SEQ ID NOS:3, 4, 7, 8, 11, 14, 17, 18, 21 and 22 are labelled with a fluorophore and a quencher as hereinbefore described according to the first-mentioned aspect.

[0084] Throughout this specification, unless the context suggests otherwise, "comprises" and "comprising" are used inclusively rather than exclusively, so that a stated integer or group of integers may include one or more other non-stated integers or groups of integers.

[0085] In the context of the present invention, by "consists essentially of" is meant that an isolated nucleic acid may consist of a stated nucleotide sequence together with one, two or three additional non-stated nucleotides within the stated nucleotide sequence and / or at a 5' or 3' end of the stated nucleotide sequence.

[0086] It will also be appreciated that the indefinite articles “a" and “an" are not to be read as singular indefinite articles or as otherwise excluding more than one or more than a single subject to which the indefinite article refers. For example, "a" primer includes one primer, one or more primers or a plurality of primers.

[0087] The term "about" is used herein to refer to a tolerance or variation in a stated amount. The tolerance or variation may be no more than ± 10%, ± 9%, ± 8%, ± 7%, ± 6%, ± 5%, ± 4%, ± 3%, ± 2% or ± 1% of a stated amount.

[0088] BRIEF DESCRIPTION OF THE FIGURES

[0089] Reference is made to non-limiting figures wherein:

[0090] FIG. 1A shows N. glabratus PCR assay performance on N. glabratus template using probe SEQ NO:4. Amplification line (HEX)=W. glabratus. Template=N. glabratus SCHI0137.M.1. No-template controls were run but are not visible due to lack of detectable signal. This assay was run in duplex format using SEQ IDs NO:1, NO:2, NO:4, NO:5, NO:6, and NO:8.

[0091] FIG. IB shows W. glabratus PCR assay performance on W. glabratus template using probe SEQ NO:3. Amplification line (HEX)=N. glabratus. Template=N. glabratus SCHI0137. M.1. No-template controls were run but are not visible due to lack of detectable signal. This assay was run in duplex format using SEQ IDs NO:1, NO:2, NO:3, NO:5, NO:6, and NO:7.

[0092] FIG. 2A shows P. kudriavzevii assay performance on P. kudriavzevii template using probe SEQ NO:8. Amplification line (FAM)=P. kudriavzevii. Template=P. kudriavzevii SCHI0087. S.155. No-template controls were run but are not visible due to lack of detectable signal. This assay was run in duplex format using SEQ IDs NO:1, NO:2, NO:4, NO:5, NO:6, and NO:8.

[0093] FIG. 2B shows P. kudriavzevii assay performance on P. kudriavzevii template using probe SEQ NO:7. Left amplification line (FAM)=P. kudriavzevii. Right amplification line (HEX)=background fluorescence, showing low-level 'pull-up' fluorescence for this probe (i.e. SEQ ID NO:3). Template=P. kudriavzevii SCHI0087. S.155. No-template controls were run but are not visible due to lack of detectable signal. This assay was run in duplex format using SEQ IDs NO:1, NO:2, NO:3, NO:5, NO:6, and NO:7. This 'pull-up' phenomenon does not occur when using the duplex format comprising SEQ IDs NO:1, NO:2, NO:4, NO:5, NO:6, and NO:8 (Fig.

[0094] 2A).

[0095] FIG. 2C shows the same as FIG. 2B, except not in log scale, to show the very low fluorescence in the HEX channel (flat line) relative to the FAM channel (curved amplification line).

[0096] FIG. 3A shows duplex PCR amplifications using a 1:1 template mix (each at 8ng / pL) of P. kudriavzevii SCHI0087. S.155 (right amplification curves) and N. glabratus SCH 10137. M.l (left amplification curves) with N. glabratus probe SEQ ID NO:4 (HEX labelled) and P. kudriavzevii probe SEQ ID NO:8 (FAM labelled) and BioRad's SsoAdvanced Universal Probes Supermix.

[0097] FIG. 3B shows the same duplex PCR amplifications described in FIG. 3A, except using PCR Biosystems' qPCRBIO Probe Mix (no-ROX). FIG 4 shows C. tropicalis PCR assay performance on C. tropicalis template. Amplification line (FAM)=C. tropicalis. Template=C. tropicalis SCHI0087. S.149. No¬ template controls were run but are not visible due to lack of detectable signal. This assay was run in duplex format using SEQ IDs NO:9, NO:10, NO:11, NO:12, NO:13, and NO:14.

[0098] FIG. 5 shows C. albicans PCR assay performance on C. albicans template. Amplification line (CAL Fluor Red 610)=C. albicans. Template=C. albicans SCHI0087. S.110. No-template controls were run but are not visible due to lack of detectable signal. This assay was run in duplex format using SEQ. IDs NO:9, NO:10, NO:11, NO:12, NO:13, and NO:14.

[0099] FIG. 6A shows duplex PCR amplifications using a 1:1 template mix (each at 8ng / pL) of C. tropicalis SCHI0087. S.149 and C. albicans SCHI0087. S.110 with C. tropicalis probe SEQ ID NO:11 (right amplification curves) and C. albicans probe SEQ ID NO:14 (left amplification curves) and Bio-Rad's SsoAdvanced Universal Probes Supermix.

[0100] FIG. 6B shows the same duplex PCR amplifications described in FIG. 6A, except using PCR Biosystems' qPCRBIO Probe Mix (no-ROX).

[0101] FIG. 7A shows C. parapsilosis PCR assay performance on C. parapsilosis template using probe SEQ NO:17. Amplification line (FAM)=C. parapsilosis. Template=C. parapsilosis SCHI0155. S.1. No-template controls were run but are not visible due to lack of detectable signal. This assay was run in duplex format using SEQ IDs NO:15, NO:16, NO:17, NO:19, NO:20, and NO:21.

[0102] FIG. 7B shows C. parapsilosis PCR assay performance on C. parapsilosis template using probe SEQ NO:18. Amplification line (FAM)=C. parapsilosis. Template=C. parapsilosis SCHI0155. S.1. No-template controls were run but are not visible due to lack of detectable signal. This assay was run in duplex format using SEQ IDs NO:15, NO:16, NO:18, NO:19, NO:20, and NO:22.

[0103] FIG. 8A shows C. dubliniensis PCR assay performance on C. dubliniensis template using probe SEQ NO:21. Amplification line (Quasar 670)=C. dubliniensis. Template=C. dubliniensis SCHI0087. S.54. No-template controls were run but are not visible due to lack of detectable signal. This assay was run in duplex format using SEQ IDs NO:15, NO:16, NO:17, NO:19, NO:20, and NO:21. FIG. 8B shows C. dubliniensis PCR assay performance on C. dubliniensis template using probe SEQ NO:22. Amplification line (HEX)=C. dubliniensis. Template=C. dubliniensis SCHI0087. S.54. No-template controls were run but are not visible due to lack of detectable signal.

[0104] FIG. 9A shows duplex PCR amplifications using a 1:1 template mix (each at 8ng / pL) of C. dubliniensis SCHI0002. S.11 and C. parapsilosis SCHI0152. S.1 with C. dubliniensis probe SEQ ID NO:22 (lower [at endpoint] amplification curves) and C. parapsilosis probe SEQ ID NO:18 (higher [at endpoint] amplification curves) and Bio¬ Rad's SsoAdvanced Universal Probes Supermix.

[0105] FIG. 9B shows the same duplex PCR amplifications described in FIG. 9A, except using PCR Biosystems' qPCRBIO Probe Mix (no-ROX).

[0106] DETAILED DESCRIPTION

[0107] The present invention provides primers and methods for detection of Nakaseomyces glabratus, Pichia kudriavzevii, Candida tropicalis, Candida albicans, Candida parapsilosis and / or Candida dubliniensis yeast by nucleic acid sequence amplification and probes which hybridize to Nakaseomyces glabratus, Pichia kudriavzevii, Candida tropicalis, Candida albicans, Candida parapsilosis and / or Candida dubliniensis amplification products.

[0108] An aspect of the invention provides an isolated nucleic acid comprising, consisting essentially of, or consisting of

[0109] a nucleotide sequence selected from the group consisting of:

[0110] (i) any one of SEQ ID NOS: 1-28;

[0111] (ii) a nucleotide sequence complementary to any of the nucleotide sequences in (ii);

[0112] (iii) a nucleotide sequence having at least 70% nucleotide sequence to any of the nucleotide sequences in (i) or (ii); and

[0113] (iv) a fragment of the isolated nucleic acids of any one of (i)-(iii).

[0114] As generally used herein, the term "yeast" refers to single-celled eukaryotic microorganisms of the Kingdom Fungi phylum Ascomycota. Particular yeasts contemplated by the present invention are pathogenic yeast, or yeast associated with pathogenicity, inclusive of genera such as Nakaseomyces, Candida and Pichia. Non-limiting examples include pathogens of humans such as Nakaseomyces glabratus, Pichia kudriavzevii, Candida tropicalis, Candida albicans, Candida parapsilosis and / or Candida dubliniensis.

[0115] For the purposes of this invention, by "isolated” is meant material that has been removed from its natural state or otherwise been subjected to human manipulation. Isolated material may be partly, substantially or essentially free from, or depleted of, components that normally accompany it in its natural state. Isolated material may be in native, chemical synthetic or recombinant form. In some embodiments, isolated material may be in enriched, partially purified or purified form.

[0116] The term "nucleic acid’ as used herein designates single- or double-stranded DNA and RNA. DNA includes genomic DNA and cDNA. RNA includes mRNA, RNA, RNAi, siRNA, cRNA, and autocatalytic RNA. Nucleic acids may also be DNA-RNA hybrids. A nucleic acid comprises a nucleotide sequence which typically includes nucleotides that comprise an A, G, C, T or U base. However, nucleotide sequences may include other bases such as inosine, thiouridine, thiothymidine, methylcytosine, methylinosine and methyladenosine, although without limitation thereto. It will also be appreciated that some of SEQ ID NOS:l-28 include nucleotide degeneracies which are indicated by standard IUPAC codes. The term "nucleic acid" also include a reverse complement nucleotide sequence of any one of SEQ ID NOS:l-28.

[0117] As used herein, a “polynucleotide" is a nucleic acid having eighty (80) or more contiguous nucleotides, while an "oligonucleotide" has less than eighty (80) contiguous nucleotides, A "probe" may be a single or double-stranded oligonucleotide or polynucleotide, preferably labelled for the purpose of detecting at least partly complementary nucleotide sequences by probe hybridization, for example. A "primer" is usually a single-stranded oligonucleotide, preferably having 15-50 contiguous nucleotides, which is capable of annealing to a complementary nucleic acid "template" and being extended in a template-dependent fashion by the action of a DNA polymerase such as Taq polymerase, RNA-dependent DNA polymerase or Sequenase™. In the context of the present invention, a "template nucleic acid" is a yeast "target nucleic acid" subjected to nucleic acid amplification. In a particular embodiment, the N. glabratus target nucleic acid encodes the Sip3p transcription cofactor in ATCC 2001 chr J (GVI51_J07799, 822329..822600); the P. kudriavzevii target nucleic acid encodes Chain A, Autophagy-related protein 18 in CY902 chromosome I (unannotated, 1184190..1184309); the C. tropicalis target nucleic acid is an unannotated region in MYA-3404 chr 3 (CP047871.1, 1205838..1205956); the C. albicans target nucleic acid is an unannotated region in SC5314 chr 1 (CP017623.1, 1562242..1562356); the C. parapsilosis target nucleic acid is CDC317 annotated contig 005504 (HE605202.1, 1028..1195) uncharacterised locus; and the target C. dubliniensis nucleic acid is CD36 chr 1 (FM992688.1, 1596149..1596263) uncharacterised locus.

[0118] Suitably, the isolated nucleic acids comprising the nucleotide sequences of SEQ ID NOS: 1 and 2 are respective forward and reverse primers suitable for nucleic acid sequence amplification of a Nakaseomyces glabratus target nucleic acid whereby nucleic acid sequence amplification produces an 87 bp amplification product (SEQ ID NO:23).

[0119] Suitably, the isolated nucleic acids comprising the nucleotide sequences of SEQ ID NOS: 5 and 6 are respective forward and reverse primers suitable for nucleic acid sequence amplification of a Pichia kudriavzevii target nucleic acid, whereby nucleic acid sequence amplification produces a 120 bp amplification product (SEQ ID NO:24).

[0120] Suitably, the isolated nucleic acids comprising the nucleotide sequences of SEQ ID NOS: 9 and 10 are respective forward and reverse primers suitable for nucleic acid sequence amplification of a Candida tropicalis target nucleic acid, whereby nucleic acid sequence amplification produces a 119bp amplification product (SEQ ID NO:25).

[0121] Suitably, the isolated nucleic acids comprising the nucleotide sequences of SEQ ID NOS:12 and 13 are respective forward and reverse primers suitable for nucleic acid sequence amplification of a Candida albicans target nucleic acid, whereby nucleic acid sequence amplification produces a 115bp amplification product (SEQ ID NO:26).

[0122] Suitably, the isolated nucleic acids comprising the nucleotide sequences of SEQ ID NOS: 15 and 16 are respective forward and reverse primers suitable for nucleic acid sequence amplification of a Candida parapsilosis target nucleic acid, whereby nucleic acid sequence amplification produces a 168bp amplification product (SEQ ID NO:27).

[0123] Suitably, the isolated nucleic acids comprising the nucleotide sequences of SEQ ID NOS: 19 and 20 are respective forward and reverse primers suitable for nucleic acid sequence amplification of a Candida dubliniensis target nucleic acid, whereby nucleic acid sequence amplification produces a 115bp amplification product (SEQ ID NO:28).

[0124] A probe comprising the nucleotide sequence set forth in SEQ ID NO:3 and / or SEQ ID NO:4 is capable of hybridizing to an amplification product produced by the primers according to SEQ ID NOS:1 and 2.

[0125] A probe comprising the nucleotide sequence set forth in SEQ ID NO:7 and / or SEQ ID NO:8 is capable of hybridizing to an amplification product produced by the primers according to SEQ ID NOS:5 and 6.

[0126] A probe comprising the nucleotide sequence set forth in SEQ ID NO:11 is capable of hybridizing to an amplification product produced by the primers according to SEQ ID NOS:9 and 10.

[0127] A probe comprising the nucleotide sequence set forth in SEQ ID NO:14 is capable of hybridizing to an amplification product produced by the primers according to SEQ ID NOS:12 and 13.

[0128] A probe comprising the nucleotide sequence set forth in SEQ ID NO:17 and / or SEQ ID NO:18 is capable of hybridizing to an amplification product produced by the primers according to SEQ ID NOS:15 and 16.

[0129] A probe comprising the nucleotide sequence set forth in SEQ ID NO:21 and / or SEQ ID NO:22 is capable of hybridizing to an amplification product produced by the primers according to SEQ ID NOS:19 and 20.

[0130] In some embodiments, the primer and / or probe may be labelled. In some embodiments, the label comprises a fluorophore.

[0131] Non-limiting examples of fluorophores include FAM, HEX, CAL Fluor Gold 540, CAL Fluor Orange 560, Quasar 670, CAL Fluor Red 610, TET, TAMRA, Texas Red, Bodipy, Rhodamine, Cy3, Cy5, LCRed and ROX fluorophores. Fluorescent probe hybridization may include fluorophore-quencher systems and / or donor-acceptor fluorophore systems. Non-limiting examples include Black Hole Quenchers, Molecular Beacons, Scorpions, Fluorescence resonance energy transfer (FRET), bioluminescence resonance energy transfer, (BRET) and TaqMan probe systems, although without limitation thereto.

[0132] A preferred quencher is a Black Hole Quencher such as BHQ-1 and / or BHQ-2. Preferably, the probes according to SEQ ID NOS:3, 4, 7, 8, 11, 14, 17, 18, 21 and / or 22 are labelled with a fluorophore and a quencher.

[0133] Non-limiting examples include:

[0134] (A) SEQ ID NO:3 - 5'-FAM or 5'-CAL Fluor Gold 540 and 3'-BHQ-1;

[0135] (B) SEQ ID NO:4 - 5'-FAM and 3'-BHQ-1;

[0136] (C) SEQ ID NO:7 - 5'-FAM and 3'-BHQ-1;

[0137] (D) SEQ ID NO:8 - 5'-FAM and 3'-BHQ-1;

[0138] (E) SEQ ID NO:11 - 5'-FAM and 3'-BHQ-1;

[0139] (F) SEQ ID NO:14 - 5'-CAL Fluor Gold 540 and 3'-BHQ-1 or 5'-CAL Fluor Red 610 and 3'-BHQ-2;

[0140] (G) SEQ ID NO:17 - 5'-FAM and 3'-BHQ-1;

[0141] (H) SEQ ID NO:18 - 5'-FAM and 3'-BHQ-1;

[0142] (I) SEQ ID NO:21 - 5'-CAL Fluor Gold 540 and 3'-BHQ-1 or 5'-Quasar 670 and 3'-BHQ-2 and;

[0143] (J) SEQ ID NO:22 - 5'-HEX and 3'-BHQ-1.

[0144] In embodiments where SEQ ID NOS 1 and 2 are used in "singleplex" assays, FAM is a preferred label.

[0145] Also contemplated are variants of the nucleotide sequences disclosed herein such as primer variants and / or variants of the probes or amplification products disclosed herein.

[0146] Accordingly, a variant nucleotide sequence may have at least 70%, 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98% or 99% nucleotide sequence identity to any one of SEQ ID NOS: 1-28.

[0147] The term "sequence identity" is used herein in its broadest sense to include the number of exact nucleotide matches having regard to an appropriate alignment using a standard algorithm, having regard to the extent that sequences are identical over a "comparison window". Thus, a "percentage of sequence identity" is calculated by comparing two optimally aligned sequences over the comparison window, determining the number of positions at which the identical nucleic acid base (e.g., A, T, C, G, I) occurs in both sequences to yield the number of matched positions, dividing the number of matched positions by the total number of positions in the window of comparison (i.e., the window size), and multiplying the result by 100 to yield the percentage of sequence identity. For example, "sequence identity" may be understood to mean the "match percentage" calculated by the DNASIS computer program (Version 2.5 for windows; available from Hitachi Software engineering Co., Ltd., South San Francisco, California, USA).

[0148] With reference to the foregoing, a "comparison window" refers to a conceptual segment of typically 6, 9 or 12 contiguous residues that is compared to a reference sequence. The comparison window may comprise additions or deletions {i.e., gaps) of about 20% or less as compared to the reference sequence for optimal alignment of the respective sequences. Optimal alignment of sequences for aligning a comparison window may be conducted by computerised implementations of algorithms (Geneworks program by Intelligenetics; GAP, BESTFIT, FASTA, and TFASTA in the Wisconsin Genetics Software Package Release 7.0, Genetics Computer Group, 575 Science Drive Madison, Wl, USA, incorporated herein by reference) or by inspection and the best alignment (i.e. resulting in the highest percentage homology over the comparison window) generated by any of the various methods selected.

[0149] Reference is also made to the BLAST family of algorithms which uses the method of Altschul et al. (1990) J. Mol. Biol. 215: 405-410), the PSI-BLAST algorithm (Nucl. Acids Res. (1997) 25 3389-3402), FASTA (which uses the method of Pearson and Lipman (1988) PNAS USA 85: 2444-2448), the Smith- Waterman algorithm (Smith and Waterman (1981) J. Mol Biol. 147: 195-197), ortheTBLASTN program, of Altschul et al. (1990) supra.

[0150] A detailed discussion of sequence analysis can be found in Unit 19.3 of CURRENT PROTOCOLS IN MOLECULAR BIOLOGY Eds. Ausubel et al. (John Wiley & Sons Inc NY, 1995-2015).

[0151] As used herein a "fragment" of an isolated nucleic acid disclosed herein is a portion, segment or sub-sequence thereof which may comprise at least about 10 contiguous nucleotides and up to about 167 contiguous nucleotides. This includes about 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95, 100, 110, 115, 120, 125, 130, 135, 140, 145, 150, 155, 160 and 165 contiguous nucleotides, or any range between these stated values.

[0152] Another aspect of the invention provides a method of amplifying a yeast target nucleic acid with one or more primers that respectively comprise, consist essentially of, or consist of the nucleotide sequences of any one or more of SEQ ID NOS: 1,2, 5, 6, 9, 10, 12, 13, 15, 16, 19 and / or 20, or that respectively comprise, consist essentially of, or consist of a nucleotide sequence having at least 70% nucleotide sequence identity to any one of SEQ. ID NOS: 1,2, 5, 6, 9, 10, 12, 13, 15, 16, 19 and / or 20, or a fragment thereof, to thereby produce an amplification product.

[0153] A further aspect of the invention provides a method of detecting the presence of absence of a yeast nucleic acid, said method including the step of subjecting a nucleic acid sample to nucleic acid sequence amplification with primers respectively comprising, consisting essentially of, or consisting of nucleotide sequences set forth in any one of more of SEQ. ID NOS: 1,2, 5, 6, 9, 10, 12, 13, 15, 16, 19 and / or 20, or that respectively comprise, consist essentially of, or consist of a nucleotide sequence having at least 70% nucleotide sequence identity to any one of SEQ ID NOS: 1,2, 5, 6, 9, 10, 12, 13, 15, 16, 19 and / or 20, or a fragment thereof, wherein the presence of an amplification product indicates that the yeast nucleic acid is present in said sample and an absence of an amplification product indicates that the yeast nucleic acid is absent from said sample.

[0154] A related aspect provides a method of detecting the presence of absence of a yeast target nucleic acid, said method including the step of subjecting a nucleic acid sample to nucleic acid sequence amplification with primers to thereby produce one or more amplification products that respectively comprise, consist essentially of, or consist of a nucleotide sequence having at least 70% nucleotide sequence identity to any one of SEQ ID NOS:23-28, or a fragment thereof, wherein the presence of an amplification product indicates that the yeast target nucleic acid is present in said sample and an absence of an amplification product indicates that the yeast target nucleic acid is absent from said sample. As generally used herein "nucleic acid amplification" refers to techniques that typically include repeated cycles of annealing one or more primers to a "template" or "target" nucleotide sequence under appropriate conditions and using a DNA polymerase to synthesize a nucleotide sequence complementary to the fungal target, thereby "amplifying" the target nucleotide sequence.

[0155] Nucleic acid amplification techniques are well known to the skilled addressee, and include thermal and isothermal amplification techniques, including but not limited to: polymerase chain reaction (PCR); strand displacement amplification (SDA); rolling circle replication (RCR); nucleic acid sequence-based amplification (NASBA), Q-β replicase amplification; helicase-dependent amplification (HDA); loop-mediated isothermal amplification (LAMP); nicking enzyme amplification reaction (NEAR) and recombinase polymerase amplification (RPA), although without limitation thereto.

[0156] PCR includes quantitative and semi-quantitative PCR (e.g. qPCR and competitive PCR), real-time PCR, allele-specific PCR, methylation-specific PCR, asymmetric PCR, nested PCR, multiplex PCR, touch-down PCR, digital PCR, droplet digital PCR, and other variations and modifications to "basic" PCR amplification.

[0157] Typically, nucleic acid sequence amplification occurs at elevated temperatures that require the use of a "thermostable" DNA polymerase that retains polymerase activity notwithstanding repeated cycles of amplification at these elevated temperatures. Non-limiting examples of thermostable DNA polymerases include Taq, KOD, Pab (Isis™), Pfu, Pst (Deep Vent™), Pwo, Tbr, Tea, Tfi, Tfl, Tfu, Tgo, TH (Vent™), Tma (UlTma™), Tne and Tth, although without limitation thereto. A nonlimiting review of the different thermostable polymerases and their properties and uses is provided by Spibida et al., 2017, J. Appl. Genet. 58: 133 and Terpe, 2013, Appl. Microbiol. Biotechnol. 97 10243.

[0158] Typically, nucleic acid amplification proceeds using multiple cycles of three sequential steps:

[0159] 1. Denaturation of the template or fungal target nucleotide sequence into single strands (or at least sufficient to enable annealing with primers) at an elevated temperature typically between about 92-98°C.

[0160] 2. Annealing one or more primers to the single strands at about 50-65°C. 3. Elongation of the annealed primers using a DNA polymerase, typically at about 60-85°C.

[0161] Generally, steps 1-3 are repeated over about 15-60 cycles.

[0162] Preferred nucleotide sequence amplification conditions are provided hereinafter in the Examples.

[0163] The aforementioned nucleic acid sequence amplification methods may be performed with one, two, three, four or five primer pairs in a single nucleic acid sequence amplification reaction. Preferably, the nucleic acid sequence amplification methods may be performed with one or two primer pairs in a single nucleic acid sequence amplification reaction.

[0164] As generally used herein, an "amplification product" or an "amplicon" refers to a nucleic acid product generated by a nucleic acid amplification technique such as described herein. Non-limiting examples of amplification products are set forth in SEQ ID NOS:19-24.

[0165] Suitably, the N. glabratus nucleic acid encodes the Sip3p transcription cofactor in ATCC 2001 chr J (GVI51_J07799, 822329..822600), whereby nucleic acid sequence amplification with respective forward and reverse primers according to SEQ ID NOS:1 and 2 produces an 87 bp amplification product (SEQ ID NO:23); the P. kudriavzevii nucleic acid encodes Chain A, Autophagy-related protein 18 in CY902 chromosome I (unannotated, 1184190..1184309), whereby nucleic acid sequence amplification with respective forward and reverse primers according to SEQ ID NOS:5 and 6 produces a 120 bp amplification product (SEQ ID NO:24); the C. tropicalis nucleic acid is an unannotated region in MYA-3404 chr 3 (CP047871.1, 1205838..1205956), whereby nucleic acid sequence amplification with respective forward and reverse primers according to SEQ ID NOS:9 and 10 produces a 119bp amplification product (SEQ ID NO:25); the C. albicans nucleic acid is an unannotated region in SC5314 chr 1 (CP017623.1, 1562242..1562356) whereby nucleic acid sequence amplification with respective forward and reverse primers according to SEQ ID NOS:12 and 13 produces a 115bp amplification product(SEQ ID NO:26); the C. parapsilosis nucleic acid is CDC317 annotated contig 005504 (HE605202.1, 1028..1195) uncharacterised locus, whereby nucleic acid sequence amplification with respective forward and reverse primers according to SEQ ID NOS:15 and 16 produces a 168bp amplification product (SEQ ID NO:27); and the C. dubliniensis nucleic acid is CD36 chr 1 (FM992688.1, 1596149..1596263) uncharacterised locus whereby nucleic acid sequence amplification with respective forward and reverse primers according to SEQ ID NOS:19 and 20 produces a 115bp amplification product (SEQ ID NO:28).

[0166] Suitably, the amplification product may be detected by direct nucleotide sequencing, agarose gel electrophoresis, detection of a fluorophore bound by the amplification product (e.g. an intercalating fluorophore such as SYBR Green I or II), hybridization of the amplification product with a probe inclusive of Northern and Southern blotting, dot blotting, slot blotting and nucleic acid arrays such as microarrays, use of labelled primers that are incorporated into the amplification product or a combination of any of these.

[0167] In a preferred embodiment, the amplification product is detected by probe hybridization. Nucleic acid probe hybridization typically includes hybridizing a nucleotide sequence of a probe to an amplification product under appropriate conditions, whereby the hybridized probe-amplification product "complex" is detected. Detection may occur in "real time" as the nucleic acid sequence amplification progresses.

[0168] According to the methods disclosed herein: a probe comprising the nucleotide sequence set forth in SEQ ID NO:3 and / or SEQ ID NO:4 hybridizes to an amplification product produced by the primers according to SEQ ID NOS:1 and 2; a probe comprising the nucleotide sequence set forth in SEQ ID NO:7 and / or SEQ ID NO:8 hybridizes to an amplification product produced by the primers according to SEQ ID NOS:5 and 6; a probe comprising the nucleotide sequence set forth in SEQ ID NO:11 hybridizes to an amplification product produced by the primers according to SEQ ID NOS:9 and 10; a probe comprising the nucleotide sequence set forth in SEQ ID NO:14 hybridizes to an amplification product produced by the primers according to SEQ ID NOS:12 and 13; a probe comprising the nucleotide sequence set forth in SEQ ID NO:17 and / or SEQ ID NO:18 hybridizes to an amplification product produced by the primers according to SEQ ID NOS:15 and 16; and a probe comprising the nucleotide sequence set forth in SEQ ID NO:21 and / or SEQ ID NO:22 hybridizes to an amplification product produced by the primers according to SEQ ID NOS:21 and 22. Suitably, the probes of SEQ ID NOS:3 and 4 are respectively capable of hybridizing to the amplification products of SEQ IS NO:23; the probes of SEQ ID NOS:7 and 8 are respectively capable of hybridizing to the amplification product of SEQ. ID NO:24; the probe of SEQ ID NO: 11 is capable of hybridizing to the amplification product of SEQ IS NO:25 the probe of SEQ ID NO:14 is capable of hybridizing to the amplification product of SEQ IS NO:26; the probes of SEQ ID NOS:17 and 18 are respectively capable of hybridizing to the amplification product of SEQ ID NO:27' and / or the probes of SEQ ID NOS:21 and 22 are respectively capable of hybridizing to the amplification product of SEQ ID NO:28.

[0169] In an embodiment, the probes of SEQ ID NOS: 3, 4, 7, 8, 11, 14, 17, 18, 21 and / or 22 are labelled, preferably with one or more fluorophores and / or quenchers. Non-limiting examples include:

[0170] (A) SEQ ID NO:3 - 5'-FAM or 5'-CAL Fluor Gold 540 and 3'-BHQ-l;

[0171] (B) SEQ ID NO:4 - 5'-FAM and 3'-BHQ-1;

[0172] (C) SEQ ID NO:7 - 5'-FAM and 3'-BHQ-1;

[0173] (D) SEQ ID NO:8 - 5'-FAM and 3'-BHQ-1;

[0174] (E) SEQ ID NO:11 - 5'-FAM and 3'-BHQ-1;

[0175] (F) SEQ ID NO:14 - 5'-CAL Fluor Gold 540 and 3'-BHQ-l or 5'-CAL Fluor Red 610 and 3'-BHQ-2;

[0176] (G) SEQ ID NO:17 - 5'-FAM and 3'-BHQ-1;

[0177] (H) SEQ ID NO:18 - 5'-FAM and 3'-BHQ-1;

[0178] (I) SEQ ID NO:21 - 5' -CAL Fluor Gold 540 and 3'-BHQ-l or 5'-Quasar 670 and 3'-BHQ-2 and;

[0179] (J) SEQ ID NO22 - 5'-HEX and BHQ-1.

[0180] In embodiments where SEQ ID NOS 1 and 2 are used in "singleplex" assays, FAM is a preferred label.

[0181] Detection of N. glabratus, P. kudriavzevii, C. tropicalis, C. albicans, C. parapsilosis and / or C. dubliniensis yeasts as disclosed herein may be performed on a sample obtained from an animal, plant, foodstuff, beverage, air, water, soil, equipment, utensils or any other potential source of yeast nucleic acids. Thus samples may be clinical, veterinary or environmental samples, although without limitation thereto. The presence or absence of yeast nucleic acids is an indicator of whether or not yeast organisms are, or have been, present in the sample source.

[0182] Preferably, methods that are performed with two or more different primer pairs ("duplex reactions") are used with purified DNA samples, whereas methods performed with a single primer pair ("singleplex reactions") may be used with clinical, environmental and / or veterinary samples to enable the detection of target yeast species in these complex samples.

[0183] Animals may include any species or other taxa that are susceptible to yeast infection, including mammals, amphibians, reptiles, fish and avians. Mammals include humans, livestock and domestic pets, although without limitation thereto. By way of example in relation to non-human animals, C. albicans, C. parapsiiosis, C. tropicalis may be pathogenic in domestic mammals (e.g. cats, cattle, dogs, horses, rodents, rabbits) and also wild mammals, avians (e.g. poultry and pet birds kept in captivity), captive reptiles, amphibians, and aquacultured fish C. dubliniensis, N. glabratus, P. kudriavzevii are more common in pet mammals (e.g dogs and cats).

[0184] In a particular embodiment, the animal is a human.

[0185] N. glabratus is a commensal of human mucosal tissues, causing difficult-to-treat invasive infections, particularly in patients with underlying conditions such as immunodeficiency, diabetes, or those who have received broad-spectrum antibiotics or chemotherapy. Infections caused by N. glabratus can affect the urogenital tract or even cause systemic infections by entrance of the fungal cells in the bloodstream (Candidemia), especially prevalent in immunocompromised patients. Infection is associated with high mortality rates and resistance to azole and echinocandin drugs is common, although N. glabratus remains mostly susceptible to amphotericin and flucytosine.

[0186] P. kudriavzevii has emerged as a fungal nosocomial pathogen primarily found in the immunocompromised and those with hematological malignancies. P. kudriavzevii has natural resistance to fluconazole, a standard antifungal agent and is often found in patients who have had prior fluconazole exposure, thus making prophylactic use of fluconazole potentially problematic. Mortality due to P. kudriavzevii is much higher than the more common C. albicans. C. albicans is an opportunistic pathogenic yeast of the human gut flora which can become pathogenic, particularly in immunocompromised individuals. Candidiasis is, for example, often observed in HIV-infected patients. There are relatively few drugs that can successfully treat candidiasis resulting from C. albicans infection, but some common treatments include amphotericin B, echinocandin, or fluconazole for systemic infections, nystatin for oral and oesophageal infections and clotrimazole for skin and genital yeast infections.

[0187] C. tropicalis is a common yeast pathogen in neutropenic hosts, in whom it may spread through the bloodstream to peripheral organs. For invasive disease, treatments include amphotericin B, echinocandins, or extended-spectrum triazole antifungals.

[0188] C. parapsilosis has become a significant cause of sepsis and of wound and tissue infections in immunocompromised humans. Unlike C. albicans and C. tropicalis, C. parapsilosis is not an obligate human pathogen, having been isolated from non-human sources such as domestic animals, insects and soil. C. parapsilosis is also a normal human commensal and it is one of the fungi most frequently isolated from human hands. Immunocompromised individuals and surgical patients, particularly those undergoing surgery of the gastrointestinal tract, are at high risk for infection with C. parapsilosis. amphotericin B and fluconazole are often used in the treatment of C. parapsilosis infection.

[0189] Candida dubliniensis commonly co-occurs with C. albicans, but these two species are impossible to differentiate based on phenotype alone.

[0190] Accordingly, a still further aspect of the invention provides a method of treating or preventing yeast colonization, infection or infestation, said method including the steps of detecting the presence of a yeast target nucleic acid according to the method of any of the aforementioned aspects and; treating the yeast colonization, infection or infestation to thereby at least partly prevent, remove, eliminate or otherwise alleviate the yeast colonization, infection or infestation.

[0191] According to this aspect, yeast may be detected in an animal or plant sample and then treated with an antifungal agent to thereby at least partly prevent, remove, eliminate or otherwise alleviate the yeast infection or infestation. In humans and other animals, antifungal agents can include polyenes (e.g., amphotericin B and nystatin), azoles including both imidazoles and triazoles (e.g ketoconazole, econazole, parconazole, fluconazole, itraconazole, isavuconazole, voriconazole, clotrimazole, tioconazole, posaconazole, miconazole), allylamines (e.g. terbinafine) and echinocandins, although without limitation thereto.

[0192] Selection and administration of the appropriate antifungal agent will be well within the ordinary practice of those skilled in the art of antifungal treatment.

[0193] Another further aspect of the invention provides a method of determining the ability of one or more primers to amplify a nucleic acid, said method including the step of comparing the respective nucleotide sequences of one or more primers according to SEQ ID NOS: 1, 2, 5, 6, 8, 9, 10, 12, 13, 15, 16, 19 and / or 20 with a nucleotide sequence of the nucleic acid to thereby determine the ability of the one or more primers to amplify the nucleic acid.

[0194] In an embodiment, the step of comparing the respective nucleotide sequences of one or more primers according to SEQ ID NOS: 1, 2, 5, 6, 8, 9, 10, 12, 13, 15, 16, 19 and / or 20 with the nucleotide sequence of the nucleic acid is performed in silico.

[0195] The method of this aspect is performed to predict whether or not the primers according SEQ ID NOS: 1, 2, 5, 6, 8, 9, 10, 12, 13, 15, 16, 19 and / or 20 will amplify a nucleic acid, such as a yeast target nucleic acid as previously described and / or amplify other "non-specific" nucleic acids, such as from other yeasts, fungi or other organisms. Accordingly, the method may provide a prediction of the expected specificity and / or sensitivity of nucleic acid sequence amplification with the primers according to SEQ ID NOS: 1, 2, 5, 6, 8, 9, 10, 12, 13, 15, 16, 19 and / or 20. Non-limiting examples of this method are described in the Examples with particular reference to Tables 1-4.

[0196] A still further aspect of the invention provides a kit comprising one or more isolated nucleic acid primers and probes comprising respective nucleotide sequences selected from SEQ ID NOS:1-22, or comprising respective nucleotide sequences at least 70% identical thereto or a fragment thereof, and optionally one or more other components selected from the group consisting of:

[0197] (a) a reverse transcriptase; (b) a thermostable DNA polymerase; and

[0198] (c) one or more detection reagents.

[0199] Preferably, the probes are labelled with a fluorophore and a quencher. Nonlimiting examples of probe fluorophore / quencher combinations are:

[0200] (A) SEQ ID NO:3 - 5'-FAM or 5'-CAL Fluor Gold 540 and 3'-BHQ-l;

[0201] (B) SEQ. ID NO:4 - 5'-FAM and 3'-BHQ-l;

[0202] (C) SEQ ID NO:7 - 5'-FAM and 3'-BHQ-1;

[0203] (D) SEQ ID NO:8 - 5'-FAM and 3'-BHQ-l:

[0204] (E) SEQ ID NO:11 - 5'-FAM and 3'-BHQ-1;

[0205] (F) SEQ ID NO:14 - 5'-CAL Fluor Gold 540 and 3'-BHQ-l or 5' -CAL Fluor Red 610 and 3'-BHQ-2;

[0206] (G) SEQ ID NO:17 - 5'-FAM and 3'-BHQ-1;

[0207] (H) SEQ ID NO:18 - 5'-FAM and 3'-BHQ-1;

[0208] (I) SEQ ID NO:21 - 5'-CAL Fluor Gold 540 and 3'-BHQ-1 or 5'-Quasar 670 and 3'-BHQ-2 and;

[0209] (J) SEQ ID NO:22 - 5'-HEX and 3'-BHQ-1.

[0210] The kit may further comprise instructions for use, one or more buffers, reaction reagents and / or other components for use in nucleic acid amplification and / or detection.

[0211] The primers, detection reagents and / or kits and method of use described herein are suitable for use in detecting yeast nucleic acids.

[0212] So that the invention may be fully understood and put into practical effect, reference is made to the following non-limiting Examples.

[0213] EXAMPLES

[0214] Example 1: Yeast Preliminary genomic analysis Prior to assay design, a yeast phylogeny was reconstructed using the Universal Fungal Core Genes (UFCG) tool (https: / / pubmed.ncbi. nlm.nih.gov / 3627195 / )to identify and correct taxonomic errors (i.e. genus and / or species misassignments), and to identify poor-quality genomes (Tables 1-4), the latter of which were discarded from further assessment. Following genome data cleaning, highly specific species-level yeast signatures were identified using the coverageBED function of BEDTools (https: / / pubmed.ncbi.nlm.nih.gov / 201110278 / ) which is wrapped in the comparative genomics tool, SPANDx v4.0.1 (https: / / pubmed.ncbi.nlm.nih.gov / 25201145 / ). This coverageBED assessment across all Candida, Nakaseomyces, and Pichia genomes was leveraged for candidate genetic signature identification by examining 1kb locus presence-absence windows to identify species-specific loci that were 100% present across the target species, but highly variable or absent in non-target species.

[0215] DNA preparation

[0216] All fungal isolates examined in vitro (Table 5) were first subcultured onto Sabouraud dextrose agar (BD Difco, Macquarie Park, NSW, Australia) to obtain single colonies. Purified cultures were directly picked using an inoculation loop. Fungal cells were incubated in 0.03 U chitinase (Sigma-Aldrich, Bayswater, VIC, Australia), 10 U achromopeptidase (Sigma-Aldrich), and 200 pL enzymatic lysis buffer (1.2% Triton-X [Bio-Rad Laboratories, South Granville, NSW, Australia) and 2X Tris-EDTA [Sigma- Aldrich], pH=8.0) at 37°C for 2 hours. For some cultures, bead beating was undertaken using a 50:50 mix of 0.1 and 0.5mm zirconia beads to assist with cellular lysis. All DNA extractions were carried out using the DNeasy Blood & Tissue kit Gram- Positive Bacteria protocol (Qiagen, Clayton, VIC, Australia), and DNA quantification was undertaken using the Qubit High Sensitivity dsDNA quantitation kit and Qubit 3 or 4 instrumentation (Invitrogen, Seventeen Mile Rocks, QLD, Australia). DNA extractions exceeding 20ng / pL were diluted to ~0.1-lng / pL to ensure reproducible qPCR amplification.

[0217] Example 2 - N. glabratus and P. kudriavzevii singleplex assays

[0218] Singleplex PCR conditions for N. glabratus assay

[0219] PCR conditions for each of 8 reactions performed as singleplex reactions were as follows: Primers

[0220] Nglabratus-f TTTCCTCGATTGTAAGTCAGGGAATC (SEQ ID NO: 1) Nglabratus-R TTCCAGCAAAATGTATAGCCCAGTC (SEQ ID NO:2)

[0221] Probe

[0222] Nglabratus-probe 5'-FAM-AAGGATCTTCATTTCCTAGAGAGAGTATATTT-3'-BHQ-1 (SEQ ID NO:3)

[0223] Reagents Initial Conc. Final conc. Vol per rxn

[0224]

[0225] Bio-Rad SsoAdvanced Universal Probes Supermix 2.0 X 1.0 X 2.500

[0226] Nglabratus-’F 20.0 pM 0.400 pM 0.100

[0227] Nglabratus-R 20.0 pM 0.400 pM 0.100

[0228] Ng la bra tus -probe 20.0 |1M 0.400 pM 0.100

[0229] * Reaction volume: 4.00 *all volumes are in pl

[0230] Thermal cycling for purified cultures was performed as follows:

[0231] Activation / denaturation: 2 min @95°C

[0232] 45 cycles of:

[0233] Denaturation: 5 sec @95°C

[0234] Annealing: 15 sec @60°C Thermal cycling for clinical specimens was performed as follows:

[0235] Activation / denaturation: 2 min @95°C

[0236] 45 cycles of:

[0237] Denaturation: 5 sec @95°C

[0238] Annealing: 15 sec @60°C

[0239] Extension: 15 sec @ 72°C

[0240] Expected amplification product

[0241] 5'- TTTCCTCGATTGTAAGTCAGGGAATCCTGTGAAGGATCTTCATTTCCTAGAGAGAGTATATTTAGAC TGGGCTATACATTTTGCTGGAA-3' (SEQ ID NO:23)

[0242] Singleplex PCR conditions for P. kudriavzevii assay

[0243] PCR conditions for each of 9 reactions performed as singleplex reactions were as follows:

[0244] Primers

[0245] Pkudriavzevii-F AGCAGAATGTCTTGCGTATAGTGAA (SEQ ID NO: 5) Pkudriavzevii-R CCTCTAATACAACCACCAACCGT (SEQ ID NO: 6)

[0246] Probe

[0247] Pkudriavzevii-probe 5'-FAM-ACCAGTAAACACACCACCATCTG-3'-BHQ-l (SEQ ID NO: 7)

[0248] Reagents Initial Cone. Final cone. Vol per rxn

[0249] H2O - - 1.163 - 1.163

[0250] Bio-Rad SsoAdvanced Universal 2.0 X 1.0 X 2.500 Probes Supermix

[0251] Pkudriavzevii-F 20.0 |1M 0.350 pM 0.088 Pkudriavzevii-R 20.0 pM 0.350 pM 0.088

[0252] Pkudriavzevii probe 20.0 pM 0.650 pM 0.163

[0253] ^Reaction volume: 4.00

[0254] all volumes are in ul.

[0255] Thermal cycling for purified cultures was performed as follows:

[0256] Activation / denaturation: 2 min @95°C

[0257] 45 cycles of:

[0258] Denaturation: 5 sec @95°C

[0259] Annealing: 15 sec @60°C

[0260] Thermal cycling for clinical specimens was performed as follows:

[0261] Activation / denaturation: 2 min @95°C

[0262] 45 cycles of:

[0263] Denaturation: 5 sec @95°C

[0264] Annealing: 15 sec @60°C

[0265] Extension: 15 sec @ 72°C

[0266] Expected amplification product

[0267] AGCAGAATGTCTTGCGTATAGTGAACACCAGTAAACACACCACCATCTGCGAGCTCTCGTACGAGT CTCCCATTATAGACGTGTCCATGAAT. AGGAAACGGTTGGTGGTTGT. ATTAGAGG-3' (SEQ ID O:24)

[0268] The results of these PCRs are shown in FIGS 2A and 2B.

[0269] Duplex PCR conditions for / V. glabratus and P. kud avzew assays PCR conditions for each of 10 reactions performed as duplex reactions were as follows:

[0270] Primers

[0271] Nglabratus-F TTTCCTCGATTGTAAGTCAGGGAATC (SEQ ID NO: 1) Nglabratus-R TTCCAGCAAAATGTATAGCCCAGTC (SEQ ID NO:2) Pkudriavzevii-P AGCAGAATGTCTTGCGTATAGTGAA (SEQ ID NO: 5) Pkudriavzevii-R CCTCTAATACAACCACCAACCGT SEQ ID NO: 6)

[0272] Probes

[0273] Nglabratus-probe CAL Fluor Gold 540-AAGGATCTTCATTTCCTAGAGAGAGTATATTT- BHQ-1 (SEQ ID NO:4)

[0274] Pkudriavzevii-probe 5'-FAM-ACCAGTAAACACACCACCATCTG-3'-BHQ-l (SEQ ID NO: 7)

[0275] Pkudriavzevii-probe 5'-FAM-CACCAGTAAACACACCACCATCT-3'-BHQ-l (SEQ ID NO: 8)

[0276] Reagents Initial Cone. Final cone. Vol per ran

[0277] H2O ■■ - 0.863 - 0.863

[0278] Bio-Rad SsoAdvanced Universal Probes Supermix 2.0 X 1.000 X 2.500

[0279] Nglabratus- 20.0 |1M 0.400 |1M 0.100

[0280] Nglabratus-R 20.0. M 0.400 jjtM 0.100

[0281] Nglabratus-probe 20.0 p. M 0.400 [iM 0.100

[0282] Pkudriavzevii -F 20.0 nM 0.350 pM 0.088

[0283] Pkudriavzevii -R 20.0 |1M 0.350 |1M 0.088 Pkudriavzevii -probe 20.0 nM 0.650 pI 0.163

[0284] *Reaction volume: 4.00

[0285] *all volumes are in p, L

[0286] Thermal cycling for purified cultures was performed as follows:

[0287] Activation / denaturation: 2 min @95°C

[0288] 45 cycles of:

[0289] Denaturation: 5 sec @95°C

[0290] Annealing: 15 sec @60°C

[0291] Thermal cycling for clinical specimens was performed as follows:

[0292] Activation / denaturation: 2 min @95°C

[0293] 45 cycles of:

[0294] Denaturation: 5 sec @95°C

[0295] Annealing: 15 sec @60°C

[0296] Extension: 15 sec @ 72°C

[0297] Expected amplification products

[0298] 5'- TTTCCTCGATTGTAAGTCAGGGAATCCTGTGAAGGATCTTCATH'CCTAGAGAGAGTATATTTAGAC TGGGCTATACATTTTGCTGGAA-3' (SEQ. ID NO:23)

[0299] 5'- AGCAGAATGTCTTGCGTATAGTGAACACCAGTAAACACACCACCATCTGCGAGCTCTCGTACGAGT CTCCCATTATAGACGTGTCCATGAATAGGAAACGGTTGGTGGTTGTATTAGAGG-3' (SEQ ID NO:24)

[0300] Duplex reactions with N. glabratus template are shown in FIG. 1.

[0301] Duplex reactions with P. kudriavzevii template are shown in FIG. 2. Duplex PCR amplifications using P. kudriavzevii SCHI0087. S.155 and N. glabratus SCHI0137. M.1 as templates are shown in FIG. 3. In FIG. 3B, this duplex PCR amplification was also performed using PCR Biosystems' qPCRBIO Probe Mix (no-ROX) instead of BioRad's SSo Advanced Universal Probes Supermix.

[0302] Example 3 - C. albicans and C. tmpicalis PCR assays

[0303] Duplex PCR conditions for C. albicans and C. tmpicalis assays

[0304] PCR conditions for each of 9 reactions performed as duplex reactions were as follows:

[0305] Primers

[0306] Ctropicalis-R GCATTATTACTCCCATTTGAGGAACG (SEO. ID NO: 9) Ctropicalis-R TTAGGATTTGCCACAAGGAACACTA (SEQ ID N0:10)

[0307] Calbicans-F TCTTGCTAGTACCATTTGTCCAATACT (SEQ ID NO:12) Calbicans-R GAGTTGTTGGGAATTGTGGTCGA (SEQ. ID NO:13)

[0308] Probes

[0309] Ctropicalis-probe 5'-FAM-TGAATTTATCCACATCATCATAGCACC-3'-BHQ-l (SEQ ID NO:11)

[0310] Calbicans-probe 5'-CAL Fluor Gold 540-C. A. ACCTC. A. ATCATCTCAAAAGCACA-3'- BHQ-2 (SEQ ID NO:14)

[0311] Reagents Initial Cone. Final cone. Vol per rxn

[0312] H2O 0.913 0.913

[0313] Bio-Rad SsoAdvanced Universal Probes Supermix 2.0 X 1.000 X 2.500 Calbicans- 20.0 |1M 0.400 pI 0.100

[0314] Calbicans-R 20.0 p. M 0.400 |1M 0.100

[0315] Calbicans-probe 20.0. M 0.300 pM 0.075

[0316] Ctrapicaiis-F 20.0 pM 0.350 pM 0.088

[0317] Ctropicalis-R 20.0 pM 0.350 pM 0.088

[0318] Ctropicalis-probe 20.0 pM 0.550 pM 0.138

[0319] Reaction volume: 4.00 all volumes are in j L

[0320] Thermal cycling for purified cultures was performed as follows:

[0321] Activation / denaturation: 2 min @95°C

[0322] 45 cycles of:

[0323] Denaturation: 5 sec @95°C

[0324] Annealing: 15 sec @60°C

[0325] Thermal cycling for clinical specimens was performed as follows:

[0326] Activation / denaturation: 2 min @95°C

[0327] 45 cycles of:

[0328] Denaturation: 5 sec @95°C

[0329] Annealing: 15 sec @60°C

[0330] Extension: 15 sec @ 72°C

[0331] Expected amplification products

[0332] 5'- G CATTATTACTCCCATTTG AG G AACG GCGG GCCAACCATCTG CTG AATTTATCCACATCATCATAG C ACCATTGTTAATGAAAAATTCAACACATAGTGTTCCTTGTGGCAAATCCTAA-3' (SEQ ID NO:25) 5'- TCTWGCTAGTACCATTTGTCCAATACTTTATAAAGTTGTTGTTTCATTCAAACCCAACCTCAATCATC TCAAAAGCACATTTTTTTGAGATTTCGACCACAATTSCCAACAACTC- 3' (SEQ ID NO:26)

[0333] The results of these PCR assays are shown in FIGS 4, 5 and 6A. In FIG. 6B;amplification of FIG. 6A was performed using PCR Biosystems' qPCRBIO Probe Mix (no-ROX) instead of BioRad's SSo Advanced Universal Probes Supermix.

[0334] Example 4 - C. p&i'&ps los s and C. dubliniensis assays

[0335] Duplex PCR conditions for C. pamps losis and C. dubliniensis assay

[0336] Primers

[0337] Cparapsilosis-'F GGTAGACCACTAATAGGTTGTATTCCA (SEQ ID N0:15) Cparapsilosis-R TTTGTGCCCGTTATTGTATGCTAAG (SEQ ID N0:16) Cdubliniensis-F GCTCCTCATGCCAITACATTTACAG (SEQ ID N0:19) Cdubiiniensis- TGTTTGCTGGAGTTGTGCCAG (SEQ ID N0:20)

[0338] Probes

[0339] Cpa rapsilosis-probe 5'-FAM-TGTCAAACAGTGTCAACTGGATACA-3'-BHQ-l (SEQID NO:17) Cd a bi in iensis- probe 5'-CAL Fluor Gold 540-AGTCATACTACAAGCCTGTCAACC-3'-BHQ-l (SEQ ID NO:21)

[0340] Cdubliniensis- probe 5'-HEX-ITTGTCAAACAGTGTCAACTGGATACA-3'-BHQ-l (SEQ ID NO:22)

[0341] PCR conditions for each of 51 reactions performed as duplex reactions were as follows: Reagents Initial Cone. Final cone. Vol per rxn

[0342] H20 0.950 - 0.950

[0343] Sso Advanced Uni Probes / lix 2.0 X 1.000 X 2.500

[0344] Cparapsilosis-F 20.0 p. M 0.350 |1M 0.088

[0345] Cparapsilosis-R 20.0. M 0.350 jjtM 0.088

[0346] Cpa rapsilosis-probe 20.0 |1M 0.600 pM 0.150

[0347] Cdubliniensis-F 20.0 nM 0.300 pI 0.088

[0348] Cdubliniensis-R 20.0 p. M 0.300 pM 0.075

[0349] Cd u bl in iensis- robe 20.0. M 0.300 pM 0.075

[0350] ^Reaction volume: 4.00 *all volumes are i;

[0351]

[0352] Thermal cycling for purified cultures was performed as follows:

[0353] Activation / denaturation: 2 min @95°C

[0354] 45 cycles of:

[0355] Denaturation: 5 sec @95°C

[0356] Annealing: 15 sec @60°C

[0357] Thermal cycling for clinical specimens was performed as follows:

[0358] Activation / denaturation: 2 min @95°C

[0359] 45 cycles of:

[0360] Denaturation: 5 sec @95°C

[0361] Annealing: 15 sec @60°C

[0362] Extension: 15 sec @ 72°C Expected amplification products

[0363] 5'- GGTAGACCACTAATAGGTTGTATTCCAAGTTTCAAAAGTATCAAGTGGATCTGTTTGTCAAACAGTG TCAACTGGATACACACATTGTAGCAAGGCCCTGCAATAAGACCCAATCATTATGATCTGCCTGCAAT ACAAAATTCCTTAGCATACAATAACGGGCACAAA-3' (SEQ ID O:27)

[0364] 5'- GCTCCTCATGCCATTACATTTACAGATAGTCATACTACAAGMCTGTCAACCCCATATGAAAAAAAAA CTTCTTACAAACCAGTTCACGTTGCAACTGGCACAACTCCAGCAAACA-3' (SEQ ID NO:28)

[0365] Further duplex PCR amplifications were performed essentially as above using C. dubliniensis SCHI0002. S.11 at 8ng / uL and C. parapsilosis SCHI0152. S.1 at 8ng / uL as templates. C. dubliniensis probe SEQ ID NO:22 was HEX labelled and C. parapsilosis probe SEQ ID NO:18 was FAM labelled.

[0366] The results of these PCRs are shown in FIGS 7-9. The duplex PCR amplification shown in FIG. 9A was also performed using PCR Biosystems' qPCRBIO Probe Mix (no-ROX) instead of BioRad's SSo Advanced Universal Probes Supermix (FIG. 9B).

[0367] Example 5 - qPCR analysis

[0368] All qPCRs were undertaken using the CFX96 Touch Real-Time PCR Detection System (Bio-Rad), SsoAdvanced Universal Probes Supermix (Bio-Rad), and IpL template DNA. All reactions were conducted in Bio-Rad optical-grade plasticware and at 5pL total reaction volume. Thermocycling conditions for all assays for purified cultures were: 95°C for 2 min, followed by 45 cycles of 95°C for 5 sec, and 60°C for 15 sec. An additional 15 sec step at 72°C was included when clinical specimen testing was undertaken. Optimised oligonucleotide concentrations for each assay are shown in the aforementioned PCR conditions, under the 'Final cone.' column. A positive control (Table 5), and no-template controls, were included on each run. All samples were run in duplicate unless otherwise specified.

[0369] Table 5 summarises the qPCR results obtained in vitro across a fungal isolate DNA panel for all assays. These results were obtained from the assays when run in duplex format. Where amplification was detected, the cycles-to-threshold values for each isolate are shown. This in vitro testing identified 100% sensitivity and specificity for each of the six tested assays.

[0370] Throughout the specification the aim has been to describe the preferred embodiments of the invention without limiting the invention to any one embodiment or specific collection of features. It will therefore be appreciated by those of skill in the art that, in light of the instant disclosure, various modifications and changes can be made in the particular embodiments exemplified without departing from the scope of the present invention.

[0371] All computer programs, algorithms, patent and scientific literature referred to herein is incorporated herein by reference. Table 1

[0372] Amplifies with

[0373] N. glabratus

[0374] Strain genome Accession no. assay in silico Comments Nakaseomyces glabratus 16.0452821 GCA_026539665.1 N / A 1 Nakaseomyces glabratus 16.6793165 GCA_026539985.1 N / A 1 Nakaseomyces glabratus CAS08-0629 SRR8697473 No 2 Nakaseomyces glabratus CG48b SRR12825218 No 3 Nakaseomyces glabratus CG48h SRR12825216 No 3 Nakaseomyces glabratus CG185 SRR12825233 No 3 Cylindricascospora (form. Nakaseomyces) bacillisporus CBS_7720 GCA_001046975.1 No 4 Cylindricascospora (form. Nakaseomyces) bacillisporus NRRL Y-17846 GCA_030573655.1 No 4 Oligophagozyma (formerly Nakaseomyces) castellii CBS_4332 GCA_001046935.1 No 4 Oligophagozyma (formerly Nakaseomyces) castellii NRRL Y-17070 GCA_030556345.1 No 4 Nakaseomyces bracarensis CBS 10154 GCA_001077315.1 No

[0375] Nakaseomyces bracarensis NRRL Y-48270 GCA_030569875.1 No

[0376] Nakaseomyces bracarensis F7312 GCA_037041435.1 No

[0377] Nakaseomyces delphensis CBS 2170 GCA_001039675.1 No

[0378] Nakaseomyces delphensis NRRL Y-2379 GCA_030570895.1 No

[0379] Nakaseomyces kungkrabaensis CBS 10927 GCA_030556385.1 No

[0380] Nakaseomyces nivariensis CBS 9983 GCA_001046915.1 No

[0381] Nakaseomyces nivariensis JHU_Cniv_vl GCA_017309295.1 No

[0382] Nakaseomyces nivariensis NRRL Y-48269 GCA_030569795.1 No

[0383] Nakaseomyces uthaithaninus 180604 SRR16974279 No

[0384] Nakaseomyces uthaithaninus CBS 10932 GCA_030564085.1 No

[0385] Nakaseomyces sp. ABEgB8 SRR7007755 No

[0386] Nakaseomyces sp. nov. ABEgT12 SRR7007756 No

[0387] Nakaseomyces sp. UFMG-CM-Y6046 SRR16974450 No

[0388] Nakaseomyces glabratus 040 GCA_004332455.1 Yes

[0389] Nakaseomyces glabratus 044 GCA_004332445.1 Yes

[0390] Nakaseomyces glabratus 15.0122390 SRR14381442 Yes

[0391] Nakaseomyces glabratus 15.0126243 SRR14381467 Yes

[0392] Nakaseomyces glabratus 15.0126255 SRR14381463 Yes

[0393] Nakaseomyces glabratus 15.0126687 SRR14381471 Yes

[0394] Nakaseomyces glabratus 15.0452362 SRR14381468 Yes

[0395] Nakaseomyces glabratus 15.0455335 SRR14381462 Yes

[0396] Nakaseomyces glabratus 15.0456221 SRR14381461 Yes

[0397] Nakaseomyces glabratus 15.0456299 SRR14381460 Yes

[0398] Nakaseomyces glabratus 15.6104001 SRR14381457 Yes

[0399] Nakaseomyces glabratus 15.6110831 SRR14381456 Yes

[0400] Nakaseomyces glabratus 16.0107051 SRR14381445 Yes

[0401] Nakaseomyces glabratus 16.0117315 SRR14381459 Yes

[0402] Nakaseomyces glabratus 16.0127919 SRR14381455 Yes

[0403] Nakaseomyces glabratus 16.0128373 SRR14381454 Yes

[0404] Nakaseomyces glabratus 16.0128490 SRR14381453 Yes

[0405] Nakaseomyces glabratus 16.0451008 SRR14381465 Yes

[0406] Nakaseomyces glabratus 16.0452821 SRR14381444 Yes Nakaseomyces glabratus 16.0453380 SRR14381446 Yes Nakaseomyces glabratus 16.0453570 SRR14381452 Yes Nakaseomyces glabratus 16.6527805 SRR14381466 Yes Nakaseomyces glabratus 16.6616386 SRR14381450 Yes Nakaseomyces glabratus 16.6715852 SRR14381469 Yes Nakaseomyces glabratus 16.6715916 SRR14381447 Yes Nakaseomyces glabratus 16.6793165 SRR14381458 Yes Nakaseomyces glabratus 17.0102191 SRR14381449 Yes Nakaseomyces glabratus 17.0450193 SRR14381448 Yes Nakaseomyces glabratus 17.6506958 SRR14381451 Yes Nakaseomyces glabratus 17.6715877 SRR14381470 Yes Nakaseomyces glabratus 17.6789296 SRR14381443 Yes Nakaseomyces glabratus 1A GCA_001466525.1 Yes Nakaseomyces glabratus 1B GCA_001466535.1 Yes Nakaseomyces glabratus 2018-F1-188-B ERR4669757 Yes Nakaseomyces glabratus 2018-F5-18 ERR4669779 Yes Nakaseomyces glabratus 2018-F6-160 ERR4669782 Yes Nakaseomyces glabratus 2018-F6-162 ERR4669784 Yes Nakaseomyces glabratus 2018-F6-164 ERR4669786 Yes Nakaseomyces glabratus 2018-F6-165 ERR4669787 Yes Nakaseomyces glabratus 2018-F6-167 ERR4669789 Yes Nakaseomyces glabratus 2A GCA_001466635.1 Yes Nakaseomyces glabratus 2B GCA_001466575.1 Yes Nakaseomyces glabratus 3A GCA_001466685.1 Yes Nakaseomyces glabratus 3B GCA_001466565.1 Yes Nakaseomyces glabratus 50570 SRR14844980 Yes Nakaseomyces glabratus 67367 SRR14844979 Yes Nakaseomyces glabratus 73281 SRR14844978 Yes Nakaseomyces glabratus AR-0314 SRR25168788 Yes Nakaseomyces glabratus AR-0316 SRR25168783 Yes Nakaseomyces glabratus AR-0318 SRR25168787 Yes Nakaseomyces glabratus AR-0319 SRR25168785 Yes Nakaseomyces glabratus AR-0320 SRR25168791 Yes Nakaseomyces glabratus AR-0322 SRR25168784 Yes Nakaseomyces glabratus AR-0323 SRR25168792 Yes Nakaseomyces glabratus AR-0324 SRR25168789 Yes Nakaseomyces glabratus AR-0325 SRR25168786 Yes Nakaseomyces glabratus AR-0326 SRR25168790 Yes Nakaseomyces glabratus AR-0327 SRR25177397 Yes Nakaseomyces glabratus AR-0328 SRR25177394 Yes Nakaseomyces glabratus AR-0329 SRR25177398 Yes Nakaseomyces glabratus AR-0330 SRR25177402 Yes Nakaseomyces glabratus AR-0581 SRR25169060 Yes Nakaseomyces glabratus AR-0582 SRR25169059 Yes Nakaseomyces glabratus AR-0583 SRR25169057 Yes Nakaseomyces glabratus AR-0584 SRR25169056 Yes Nakaseomyces glabratus AR-0585 SRR25169058 Yes Nakaseomyces glabratus ATCC 2001 GCA_010111755.1 Yes Nakaseomyces glabratus ATCC2001_CBS138 SRR10717941 Yes Nakaseomyces glabratus B1514 GCA_023629155.1 Yes Nakaseomyces glabratus BG2 GCA_014217725.1 Yes Nakaseomyces glabratus BG2_11B_AinF SRR15498445 Yes Nakaseomyces glabratus BG2_11B_ANI SRR15498429 Yes Nakaseomyces glabratus BG2_11B_FinA SRR15498477 Yes Nakaseomyces glabratus BG2_11B_FLZ SRR15498478 Yes Nakaseomyces glabratus BG2_11B_YPD SRR15498489 Yes Nakaseomyces glabratus BG2_11H_AinF SRR15498492 Yes Nakaseomyces glabratus BG2_11H_ANI SRR15498443 Yes Nakaseomyces glabratus BG2_11H_FinA SRR15498475 Yes Nakaseomyces glabratus BG2_11H_FLZ SRR15498461 Yes Nakaseomyces glabratus BG2_SRA_WT SRR15498448 Yes Nakaseomyces glabratus BG3993 GCA_020450195.1 Yes Nakaseomyces glabratus BG3994 GCA_020450265.1 Yes Nakaseomyces glabratus BG3995 GCA_020450215.1 Yes Nakaseomyces glabratus BG3996 GCA_020450235.1 Yes Nakaseomyces glabratus C10471 SRR23984272 Yes Nakaseomyces glabratus C10472 SRR23984271 Yes Nakaseomyces glabratus C10473 SRR23984270 Yes Nakaseomyces glabratus C10474 SRR23984269 Yes Nakaseomyces glabratus C10534 SRR23984268 Yes Nakaseomyces glabratus C10535 SRR23984264 Yes Nakaseomyces glabratus C10536 SRR23984267 Yes Nakaseomyces glabratus C10537 SRR23984266 Yes Nakaseomyces glabratus C10538 SRR23984265 Yes Nakaseomyces glabratus C10539 SRR23984263 Yes Nakaseomyces glabratus CAGL1256 SRR13441714 Yes Nakaseomyces glabratus CAGL1875 SRR13441715 Yes Nakaseomyces glabratus CAS08-0016 SRR14180811 Yes Nakaseomyces glabratus CAS08-0027 SRR14180815 Yes Nakaseomyces glabratus CAS08-0069 SRR8697397 Yes Nakaseomyces glabratus CAS08-0123 SRR8697376 Yes Nakaseomyces glabratus CAS08-0136 SRR8697452 Yes Nakaseomyces glabratus CAS08-0154 SRR8697454 Yes Nakaseomyces glabratus CAS08-0173 SRR8697407 Yes Nakaseomyces glabratus CAS08-0190 SRR8697408 Yes Nakaseomyces glabratus CAS08-0209 SRR8697364 Yes Nakaseomyces glabratus CAS08-0222 SRR8697383 Yes Nakaseomyces glabratus CAS08-0248 SRR8697402 Yes Nakaseomyces glabratus CAS08-0313 SRR8697403 Yes Nakaseomyces glabratus CAS08-0317 SRR8697474 Yes Nakaseomyces glabratus CAS08-0343 SRR8697334 Yes Nakaseomyces glabratus CAS08-0407 SRR8697439 Yes Nakaseomyces glabratus CAS08-0423 SRR8697347 Yes Nakaseomyces glabratus CAS08-0425 SRR14180816 Yes Nakaseomyces glabratus CAS08-0456 SRR8697379 Yes Nakaseomyces glabratus CAS08-0458 SRR8697393 Yes Nakaseomyces glabratus CAS08-0482 SRR8697333 Yes Nakaseomyces glabratus CAS08-0525 SRR8697425 Yes Nakaseomyces glabratus CAS08-0557 SRR8697366 Yes Nakaseomyces glabratus CAS08-0559 SRR8697483 Yes Nakaseomyces glabratus CAS08-0569 SRR8697493 Yes Nakaseomyces glabratus CAS08-0616 SRR8697343 Yes Nakaseomyces glabratus CAS08-0627 SRR8697427 Yes Nakaseomyces glabratus CAS08-0647 SRR8697428 Yes Nakaseomyces glabratus CAS08-0725 SRR8697475 Yes Nakaseomyces glabratus CAS09-0751 SRR8697308 Yes Nakaseomyces glabratus CAS09-0811 SRR8697466 Yes Nakaseomyces glabratus CAS09-0818 SRR8697461 Yes Nakaseomyces glabratus CAS09-0872 SRR8697309 Yes Nakaseomyces glabratus CAS09-0901 SRR8697302 Yes Nakaseomyces glabratus CAS09-0930 SRR8697412 Yes Nakaseomyces glabratus CAS09-0993 SRR8697424 Yes Nakaseomyces glabratus CAS09-0997 SRR8697372 Yes Nakaseomyces glabratus CAS09-1021 SRR8697327 Yes Nakaseomyces glabratus CAS09-1091 SRR8697328 Yes Nakaseomyces glabratus CAS09-1195 SRR8697367 Yes Nakaseomyces glabratus CAS09-1352 SRR8697492 Yes Nakaseomyces glabratus CAS09-1450 SRR8697441 Yes Nakaseomyces glabratus CAS09-1513 SRR8697489 Yes Nakaseomyces glabratus CAS09-1601 SRR8697387 Yes Nakaseomyces glabratus CAS09-1622 SRR8697371 Yes Nakaseomyces glabratus CAS09-1798 SRR8697346 Yes Nakaseomyces glabratus CAS09-1809 SRR8697354 Yes Nakaseomyces glabratus CAS10-1832 SRR8697494 Yes Nakaseomyces glabratus CAS10-1862 SRR8697396 Yes Nakaseomyces glabratus CAS10-1893 SRR8697384 Yes Nakaseomyces glabratus CAS10-1895 SRR8697480 Yes Nakaseomyces glabratus CAS10-1919 SRR8697490 Yes Nakaseomyces glabratus CAS10-1922 SRR8697414 Yes Nakaseomyces glabratus CAS10-1929 SRR8697270 Yes Nakaseomyces glabratus CAS10-1964 SRR8697267 Yes Nakaseomyces glabratus CAS10-1984 SRR8697458 Yes Nakaseomyces glabratus CAS10-1986 SRR8697394 Yes Nakaseomyces glabratus CAS10-2006 SRR8697395 Yes Nakaseomyces glabratus CAS10-2124 SRR8697271 Yes Nakaseomyces glabratus CAS10-2289 SRR8697352 Yes Nakaseomyces glabratus CAS10-2308 SRR8697365 Yes Nakaseomyces glabratus CAS10-2449 SRR8697479 Yes Nakaseomyces glabratus CAS10-2472 SRR8697434 Yes Nakaseomyces glabratus CAS10-2476 SRR8697449 Yes Nakaseomyces glabratus CAS10-2478 SRR8697382 Yes Nakaseomyces glabratus CAS10-2533 SRR8697377 Yes Nakaseomyces glabratus CAS10-2547 SRR8697433 Yes Nakaseomyces glabratus CAS10-2558 SRR8697411 Yes Nakaseomyces glabratus CAS10-2559 SRR8697443 Yes Nakaseomyces glabratus CAS10-2592 SRR8697363 Yes Nakaseomyces glabratus CAS10-2626 SRR8697410 Yes Nakaseomyces glabratus CAS10-2717 SRR8697272 Yes Nakaseomyces glabratus CAS10-2720 SRR8697321 Yes Nakaseomyces glabratus CAS10-2732 SRR8697349 Yes Nakaseomyces glabratus CAS11-2802 SRR8697436 Yes Nakaseomyces glabratus CAS11-2817 SRR8697465 Yes Nakaseomyces glabratus CAS11-2831 SRR8697447 Yes Nakaseomyces glabratus CAS11-2834 SRR8697457 Yes Nakaseomyces glabratus CAS11-2853 SRR8697276 Yes Nakaseomyces glabratus CAS11-2870 SRR8697374 Yes Nakaseomyces glabratus CAS11-2931 SRR8697368 Yes Nakaseomyces glabratus CAS11-2934 SRR8697341 Yes Nakaseomyces glabratus CAS11-2972 SRR8697342 Yes Nakaseomyces glabratus CAS11-2978 SRR8697340 Yes Nakaseomyces glabratus CAS11-2985 SRR8697472 Yes Nakaseomyces glabratus CAS11-3018 SRR8697462 Yes Nakaseomyces glabratus CAS11-3122 SRR8697467 Yes Nakaseomyces glabratus CAS11-3129 SRR8697269 Yes Nakaseomyces glabratus CAS11-3232 SRR8697344 Yes Nakaseomyces glabratus CAS11-3233 SRR8697456 Yes Nakaseomyces glabratus CAS11-3234 SRR8697463 Yes Nakaseomyces glabratus CAS11-3247 SRR8697437 Yes Nakaseomyces glabratus CAS11-3264 SRR8697331 Yes Nakaseomyces glabratus CAS11-3345 SRR8697320 Yes Nakaseomyces glabratus CAS11-3395 SRR8697476 Yes Nakaseomyces glabratus CAS11-3433 SRR8697401 Yes Nakaseomyces glabratus CAS11-3481 SRR8697369 Yes Nakaseomyces glabratus CAS11-3482 SRR8697375 Yes Nakaseomyces glabratus CAS12-3674 SRR8697350 Yes Nakaseomyces glabratus CAS12-3684 SRR8697392 Yes Nakaseomyces glabratus CAS12-3690 SRR8697405 Yes Nakaseomyces glabratus CAS12-3692 SRR8697429 Yes Nakaseomyces glabratus CAS12-3708 SRR8697477 Yes Nakaseomyces glabratus CAS12-3737 SRR8697300 Yes Nakaseomyces glabratus CAS12-3739 SRR8697419 Yes Nakaseomyces glabratus CAS12-3900 SRR8697481 Yes Nakaseomyces glabratus CAS12-3946 SRR8697358 Yes Nakaseomyces glabratus CAS12-3951 SRR8697353 Yes Nakaseomyces glabratus CAS12-3980 SRR8697448 Yes Nakaseomyces glabratus CAS12-3983 SRR8697404 Yes Nakaseomyces glabratus CAS12-3998 SRR8697362 Yes Nakaseomyces glabratus CAS12-4046 SRR8697471 Yes Nakaseomyces glabratus CAS12-4099 SRR8697389 Yes Nakaseomyces glabratus CAS12-4162 SRR8697399 Yes Nakaseomyces glabratus CAS12-4163 SRR8697453 Yes Nakaseomyces glabratus CAS12-4176 SRR8697264 Yes Nakaseomyces glabratus CAS12-4212 SRR8697275 Yes Nakaseomyces glabratus CAS12-4215 SRR8697426 Yes Nakaseomyces glabratus CAS12-4242 SRR8697361 Yes Nakaseomyces glabratus CAS12-4323 SRR8697451 Yes Nakaseomyces glabratus CAS12-4380 SRR8697317 Yes Nakaseomyces glabratus CAS12-4440 SRR8697303 Yes Nakaseomyces glabratus CAS13-4493 SRR8697325 Yes Nakaseomyces glabratus CAS13-4530 SRR8697440 Yes Nakaseomyces glabratus CAS13-4641 SRR8697417 Yes Nakaseomyces glabratus CAS13-4648 SRR8697335 Yes Nakaseomyces glabratus CAS13-4735 SRR8697388 Yes Nakaseomyces glabratus CAS13-4776 SRR8697373 Yes Nakaseomyces glabratus CAS13-4777 SRR8697322 Yes Nakaseomyces glabratus CAS13-4778 SRR8697273 Yes Nakaseomyces glabratus CAS13-4791 SRR8697360 Yes Nakaseomyces glabratus CAS13-4813 SRR8697323 Yes Nakaseomyces glabratus CAS13-4841 SRR8697386 Yes Nakaseomyces glabratus CAS13-4862 SRR8697378 Yes Nakaseomyces glabratus CAS13-4878 SRR8697398 Yes Nakaseomyces glabratus CAS13-4970 SRR8697416 Yes Nakaseomyces glabratus CAS13-5030 SRR8697406 Yes Nakaseomyces glabratus CAS13-5037 SRR8697277 Yes Nakaseomyces glabratus CAS13-5109 SRR8697345 Yes Nakaseomyces glabratus CAS13-5134 SRR8697307 Yes Nakaseomyces glabratus CAS13-5140 SRR8697464 Yes Nakaseomyces glabratus CAS13-5158 SRR8697488 Yes Nakaseomyces glabratus CAS13-5210 SRR8697444 Yes Nakaseomyces glabratus CAS13-5237 SRR8697418 Yes Nakaseomyces glabratus CAS13-5239 SRR8697324 Yes Nakaseomyces glabratus CAS13-5245 SRR8697445 Yes Nakaseomyces glabratus CAS14-5293 SRR8697478 Yes Nakaseomyces glabratus CAS14-5468 SRR8697381 Yes Nakaseomyces glabratus CAS14-5492 SRR8697430 Yes Nakaseomyces glabratus CAS14-5518 SRR8697413 Yes Nakaseomyces glabratus CAS14-5563 SRR8697482 Yes Nakaseomyces glabratus CAS14-5589 SRR8697319 Yes Nakaseomyces glabratus CAS14-5591 SRR8697468 Yes Nakaseomyces glabratus CAS14-5647 SRR8697491 Yes Nakaseomyces glabratus CAS14-5770 SRR8697356 Yes Nakaseomyces glabratus CAS14-5851 SRR8697329 Yes Nakaseomyces glabratus CAS14-5908 SRR8697355 Yes Nakaseomyces glabratus CAS14-5982 SRR8697351 Yes Nakaseomyces glabratus CAS14-5984 SRR8697422 Yes Nakaseomyces glabratus CAS14-5989 SRR8697370 Yes Nakaseomyces glabratus CAS14-6054 SRR8697326 Yes Nakaseomyces glabratus CAS15-6171 SRR8697469 Yes Nakaseomyces glabratus CAS15-6184 SRR8697495 Yes Nakaseomyces glabratus CAS15-6272 SRR8697301 Yes Nakaseomyces glabratus CAS15-6297 SRR8697460 Yes Nakaseomyces glabratus CAS15-6327 SRR8697304 Yes Nakaseomyces glabratus CAS15-6394 SRR8697390 Yes Nakaseomyces glabratus CAS15-6404 SRR8697409 Yes Nakaseomyces glabratus CAS15-6472 SRR8697450 Yes Nakaseomyces glabratus CAS15-6569 SRR8697336 Yes Nakaseomyces glabratus CAS15-6616 SRR8697306 Yes Nakaseomyces glabratus CAS15-6673 SRR8697330 Yes Nakaseomyces glabratus CAS15-6682 SRR8697438 Yes Nakaseomyces glabratus CAS15-6812 SRR8697455 Yes Nakaseomyces glabratus CAS15-6814 SRR8697446 Yes Nakaseomyces glabratus CAS16-6915 SRR8697332 Yes Nakaseomyces glabratus CAS16-6927 SRR8697338 Yes Nakaseomyces glabratus CAS16-6951 SRR8697357 Yes Nakaseomyces glabratus CAS16-7048 SRR8697359 Yes Nakaseomyces glabratus CAS16-7105 SRR8697421 Yes Nakaseomyces glabratus CAS16-7155 SRR8697299 Yes Nakaseomyces glabratus CAS16-7332 SRR8697470 Yes Nakaseomyces glabratus CAS16-7378 SRR8697268 Yes Nakaseomyces glabratus CAS16-7380 SRR8697431 Yes Nakaseomyces glabratus CAS16-7403 SRR8697266 Yes Nakaseomyces glabratus CAS16-7419 SRR8697385 Yes Nakaseomyces glabratus CAS16-7440 SRR8697339 Yes Nakaseomyces glabratus CAS16-7442 SRR8697337 Yes Nakaseomyces glabratus CAS16-7574 SRR8697442 Yes Nakaseomyces glabratus CAS16-7575 SRR8697432 Yes Nakaseomyces glabratus CAS16-7596 SRR8697274 Yes Nakaseomyces glabratus CAS17-7689 SRR8697400 Yes Nakaseomyces glabratus CAS17-7704 SRR8697423 Yes Nakaseomyces glabratus CAS17-7712 SRR8697348 Yes Nakaseomyces glabratus CAS17-7728 SRR8697435 Yes Nakaseomyces glabratus CAS17-7768 SRR8697278 Yes Nakaseomyces glabratus CBS138 GCA_000002545.2 Yes Nakaseomyces glabratus CBS138_9F_AinF SRR15498495 Yes Nakaseomyces glabratus CBS138_9F_ANI SRR15498425 Yes Nakaseomyces glabratus CBS138_9F_FinA SRR15498474 Yes Nakaseomyces glabratus CBS138_9F_FLZ SRR15498497 Yes Nakaseomyces glabratus CBS138_9F_YPD SRR15498479 Yes Nakaseomyces glabratus CBS138_9H_AinF SRR15498485 Yes Nakaseomyces glabratus CBS138_9H_ANI SRR15498449 Yes Nakaseomyces glabratus CBS138_9H_FinA SRR15498483 Yes Nakaseomyces glabratus CBS138_9H_FLZ SRR15498471 Yes Nakaseomyces glabratus CBS138_SRA_WT SRR15498470 Yes Nakaseomyces glabratus CCTCC_M202019 GCA_000497105.2 Yes Nakaseomyces glabratus CG107a SRR12825230 Yes Nakaseomyces glabratus CG107b SRR12825229 Yes Nakaseomyces glabratus CG121 SRR12825250 Yes Nakaseomyces glabratus CG124 SRR12825249 Yes Nakaseomyces glabratus CG127 SRR12825248 Yes Nakaseomyces glabratus CG133 SRR12825247 Yes Nakaseomyces glabratus CG134 SRR12825246 Yes Nakaseomyces glabratus CG144 SRR12825245 Yes Nakaseomyces glabratus CG146 SRR12825244 Yes Nakaseomyces glabratus CG150 SRR12825242 Yes Nakaseomyces glabratus CG151 SRR12825241 Yes Nakaseomyces glabratus CG153 SRR12825240 Yes Nakaseomyces glabratus CG157 SRR12825239 Yes Nakaseomyces glabratus CG162 SRR12825238 Yes Nakaseomyces glabratus CG166 SRR12825237 Yes Nakaseomyces glabratus CG167 SRR12825236 Yes Nakaseomyces glabratus CG174 SRR12825235 Yes Nakaseomyces glabratus CG181 SRR12825234 Yes Nakaseomyces glabratus CG18a SRR12825228 Yes Nakaseomyces glabratus CG18b SRR12825227 Yes Nakaseomyces glabratus CG191a SRR12825226 Yes Nakaseomyces glabratus CG191b SRR12825225 Yes Nakaseomyces glabratus CG191c SRR12825224 Yes Nakaseomyces glabratus CG191d SRR12825223 Yes Nakaseomyces glabratus CG191e SRR12825222 Yes Nakaseomyces glabratus CG191f SRR12825220 Yes Nakaseomyces glabratus CG203 SRR12825231 Yes Nakaseomyces glabratus CG42 SRR12825243 Yes Nakaseomyces glabratus CG43 SRR12825232 Yes Nakaseomyces glabratus CG46 SRR12825221 Yes Nakaseomyces glabratus CG48a SRR12825219 Yes Nakaseomyces glabratus CG48f SRR12825217 Yes Nakaseomyces glabratus CG52 SRR12825210 Yes Nakaseomyces glabratus CG53 SRR12825199 Yes Nakaseomyces glabratus CG54 SRR12825196 Yes Nakaseomyces glabratus CG55 SRR12825195 Yes Nakaseomyces glabratus CG68a SRR12825215 Yes Nakaseomyces glabratus CG69b SRR12825214 Yes Nakaseomyces glabratus CG84f SRR12825213 Yes Nakaseomyces glabratus CG84g SRR12825212 Yes Nakaseomyces glabratus CG84h SRR12825211 Yes Nakaseomyces glabratus CG93a SRR12825209 Yes Nakaseomyces glabratus CG93b SRR12825208 Yes Nakaseomyces glabratus CG93c SRR12825207 Yes Nakaseomyces glabratus CG93d SRR12825206 Yes Nakaseomyces glabratus CG93e SRR12825205 Yes Nakaseomyces glabratus CG93h SRR12825204 Yes Nakaseomyces glabratus CG93i SRR12825203 Yes Nakaseomyces glabratus CG93j SRR12825202 Yes Nakaseomyces glabratus CG93k SRR12825201 Yes Nakaseomyces glabratus CG97a SRR12825200 Yes Nakaseomyces glabratus CG97b SRR12825198 Yes Nakaseomyces glabratus CG97c SRR12825197 Yes Nakaseomyces glabratus CLIB_3042 ERR9706985 Yes Nakaseomyces glabratus CST34_2A_AinF SRR15498503 Yes Nakaseomyces glabratus CST34_2A_ANI SRR15498440 Yes Nakaseomyces glabratus CST34_2A_ANIFLZ SRR15498473 Yes Nakaseomyces glabratus CST34_2A_FinA SRR15498430 Yes Nakaseomyces glabratus CST34_2A_FLZ SRR15498458 Yes Nakaseomyces glabratus CST34_2A_YPD SRR15498463 Yes Nakaseomyces glabratus CST34_2C_FinA SRR15498462 Yes Nakaseomyces glabratus CST34_2C_FLZ SRR15498499 Yes Nakaseomyces glabratus CST34_2G_AinF SRR15498457 Yes Nakaseomyces glabratus CST34_2G_ANI SRR15498451 Yes Nakaseomyces glabratus CST34_2G_ANIFLZ SRR15498467 Yes Nakaseomyces glabratus CST78_4G_FinA SRR15498434 Yes Nakaseomyces glabratus CST78_4G_FLZ SRR15498496 Yes Nakaseomyces glabratus CST78_4G_YPD SRR15498493 Yes Nakaseomyces glabratus DPK305 SRR14180810 Yes Nakaseomyces glabratus DPK762 SRR14180814 Yes Nakaseomyces glabratus DPL1021 SRR14180813 Yes Nakaseomyces glabratus DPL245 SRR14180812 Yes Nakaseomyces glabratus DSY562 GCA_002219185.1 Yes Nakaseomyces glabratus DSY565 GCA_002219195.1 Yes Nakaseomyces glabratus EB0911_3B_AinF SRR15498488 Yes Nakaseomyces glabratus EB0911_3B_ANI SRR15498427 Yes Nakaseomyces glabratus EB0911_3B_FinA SRR15498452 Yes Nakaseomyces glabratus EB0911_3B_FLZ SRR15498436 Yes Nakaseomyces glabratus EB0911_3B_YPD SRR15498472 Yes Nakaseomyces glabratus EB0911_3H_AinF SRR15498494 Yes Nakaseomyces glabratus EB0911_3H_ANI SRR15498464 Yes Nakaseomyces glabratus EB0911_3H_FinA SRR15498444 Yes Nakaseomyces glabratus EB0911_3H_FLZ SRR15498484 Yes Nakaseomyces glabratus EB0911Sto SRR15498487 Yes Nakaseomyces glabratus EF1620_7B_AinF SRR15498460 Yes Nakaseomyces glabratus EF1620_7B_ANI SRR15498456 Yes Nakaseomyces glabratus EF1620_7B_FinA SRR15498480 Yes Nakaseomyces glabratus EF1620_7B_FLZ SRR15498439 Yes Nakaseomyces glabratus EF1620_7D_AinF SRR15498438 Yes Nakaseomyces glabratus EF1620_7D_ANI SRR15498482 Yes Nakaseomyces glabratus EF1620_7D_ANIFLZ SRR15498466 Yes Nakaseomyces glabratus EF1620_7D_FinA SRR15498500 Yes Nakaseomyces glabratus EF1620_7D_FLZ SRR15498476 Yes Nakaseomyces glabratus EF1620_7D_YPD SRR15498465 Yes Nakaseomyces glabratus EF1620_7F_AinF SRR15498432 Yes Nakaseomyces glabratus EF1620_7F_ANI SRR15498431 Yes Nakaseomyces glabratus EF1620_7F_ANIFLZ SRR15498490 Yes Nakaseomyces glabratus ERZ1036435.336 GCA_937870575.1 Yes Nakaseomyces glabratus F15_8G_FinA SRR15498455 Yes Nakaseomyces glabratus F15_8G_FLZ SRR15498447 Yes Nakaseomyces glabratus F15_8G_YPD SRR15498423 Yes Nakaseomyces glabratus F15_SRA_WT SRR15498486 Yes Nakaseomyces glabratus FFUL887 GCA_900166875.1 Yes Nakaseomyces glabratus G_18_l SRR19696157 Yes Nakaseomyces glabratus G_18_2 SRR19696149 Yes Nakaseomyces glabratus G_18_3 SRR19696147 Yes Nakaseomyces glabratus G_18_4 SRR19696146 Yes Nakaseomyces glabratus G_18_5 SRR19696145 Yes Nakaseomyces glabratus G_18_6 SRR19696144 Yes Nakaseomyces glabratus G_18_7 SRR19696143 Yes Nakaseomyces glabratus G_18_8 SRR19696142 Yes Nakaseomyces glabratus G_18_9 SRR19696141 Yes Nakaseomyces glabratus G_18_10 SRR19696156 Yes Nakaseomyces glabratus G_18_11 SRR19696155 Yes Nakaseomyces glabratus G_18_12 SRR19696154 Yes Nakaseomyces glabratus G_18_13 SRR19696153 Yes Nakaseomyces glabratus G_18_14 SRR19696152 Yes Nakaseomyces glabratus G_18_15 SRR19696151 Yes Nakaseomyces glabratus G_18_16 SRR19696150 Yes Nakaseomyces glabratus G_19_1 SRR19696140 Yes Nakaseomyces glabratus G_19_2 SRR19696092 Yes Nakaseomyces glabratus G_19_3 SRR19696088 Yes Nakaseomyces glabratus G_19_4 SRR19696087 Yes Nakaseomyces glabratus G_19_5 SRR19696086 Yes Nakaseomyces glabratus G_19_6 SRR19696085 Yes Nakaseomyces glabratus G_19_7 SRR19696084 Yes Nakaseomyces glabratus G_19_8 SRR19696083 Yes Nakaseomyces glabratus G_19_9 SRR19696082 Yes Nakaseomyces glabratus G_19_10 SRR19696139 Yes Nakaseomyces glabratus G_19_11 SRR19696138 Yes Nakaseomyces glabratus G_19_12 SRR19696136 Yes Nakaseomyces glabratus G_19_13 SRR19696135 Yes Nakaseomyces glabratus G_19_14 SRR19696134 Yes Nakaseomyces glabratus G_19_15 SRR19696133 Yes Nakaseomyces glabratus G_19_16 SRR19696132 Yes Nakaseomyces glabratus G_19_17 SRR19696131 Yes Nakaseomyces glabratus G_19_18 SRR19696130 Yes Nakaseomyces glabratus G_19_19 SRR19696093 Yes Nakaseomyces glabratus G_19_20 SRR19696091 Yes Nakaseomyces glabratus G_19_21 SRR19696089 Yes Nakaseomyces glabratus G_20_1 SRR19696081 Yes Nakaseomyces glabratus G_20_2 SRR19696074 Yes Nakaseomyces glabratus G_20_3 SRR19696073 Yes Nakaseomyces glabratus G_20_4 SRR19696072 Yes Nakaseomyces glabratus G_20_6 SRR19696071 Yes Nakaseomyces glabratus G_20_7 SRR19696070 Yes Nakaseomyces glabratus G_20_8 SRR19696069 Yes Nakaseomyces glabratus G_20_9 SRR19696265 Yes Nakaseomyces glabratus G_20_10 SRR19696080 Yes Nakaseomyces glabratus G_20_11 SRR19696078 Yes Nakaseomyces glabratus G_20_12 SRR19696077 Yes Nakaseomyces glabratus G_20_13 SRR19696076 Yes Nakaseomyces glabratus G_20_14 SRR19696075 Yes Nakaseomyces glabratus G_21_1A SRR19696264 Yes Nakaseomyces glabratus G_21_2 SRR19696263 Yes Nakaseomyces glabratus G_21_3 SRR19696262 Yes Nakaseomyces glabratus G_21_4 SRR19696261 Yes Nakaseomyces glabratus G_21_5A SRR19696260 Yes Nakaseomyces glabratus IHEM_26304 SRR10725597 Yes Nakaseomyces glabratus IHEM_9556 SRR10725599 Yes Nakaseomyces glabratus ISTA29 SRR13648885 Yes Nakaseomyces glabratus ISTB218 SRR13648884 Yes Nakaseomyces glabratus M12_5F_AinF SRR15498501 Yes Nakaseomyces glabratus M12_5F_ANI SRR15498481 Yes Nakaseomyces glabratus M12_5F_FinA SRR15498437 Yes Nakaseomyces glabratus M12_5F_YPD SRR15498450 Yes Nakaseomyces glabratus M12_5H_AinF SRR15498426 Yes Nakaseomyces glabratus M12_5H_ANI SRR15498453 Yes Nakaseomyces glabratus M12_5H_FinA SRR15498433 Yes Nakaseomyces glabratus M12_5H_FLZ SRR15498446 Yes Nakaseomyces glabratus M1215 GCA_023629135.1 Yes Nakaseomyces glabratus M6 GCA_025766415.1 Yes Nakaseomyces glabratus ML72254 SRR17163935 Yes Nakaseomyces glabratus MY07 GCA_022702475.2 Yes Nakaseomyces glabratus NRRL Y-65 SRR16974273 Yes Nakaseomyces glabratus OL152 GCA_004332465.1 Yes Nakaseomyces glabratus P35_10E_AinF SRR15498498 Yes Nakaseomyces glabratus P35_10E_ANI SRR15498454 Yes Nakaseomyces glabratus P35_10E_ANIFLZ SRR15498441 Yes Nakaseomyces glabratus P35_10E_FinA SRR15498468 Yes Nakaseomyces glabratus P35_10E_FLZ SRR15498435 Yes Nakaseomyces glabratus P35_10E_YPD SRR15498469 Yes Nakaseomyces glabratus P35_10G_AinF SRR15498428 Yes Nakaseomyces glabratus P35_10G_ANI SRR15498459 Yes Nakaseomyces glabratus P35_10G_ANIFLZ SRR15498424 Yes Nakaseomyces glabratus P35_10G_FinA SRR15498491 Yes Nakaseomyces glabratus P35_10G_FLZ SRR15498442 Yes Nakaseomyces glabratus P35-2 GCA_025331905.1 Yes Nakaseomyces glabratus PK3901 GCA_025503555.1 Yes Nakaseomyces glabratus PUJ-HUSI_916 SRR13441713 Yes Nakaseomyces glabratus SCHI0137. M.1 GCA_044592685.1 Yes Nakaseomyces glabratus SP1643 SRR11235429 Yes Nakaseomyces glabratus SP2320 SRR112354280 Yes Nakaseomyces glabratus SP2659 SRR11235427 Yes Nakaseomyces glabratus SP2982 SRR112354228 Yes Nakaseomyces glabratus SP3003 SRR11235421 Yes Nakaseomyces glabratus SP3046 SRR11235424 Yes Nakaseomyces glabratus SP3417 SRR11235426 Yes Nakaseomyces glabratus SP3439 SRR11235423 Yes Nakaseomyces glabratus SP3689 SRR11235425 Yes Nakaseomyces glabratus UAB047-W10D4 GCA 002259835.1 Yes Nakaseomyces glabratus UHNDB10192 GCA_021498375.1 Yes Nakaseomyces glabratus UHNDB8982 GCA_021498365.1 Yes Nakaseomyces glabratus UHNDB9150 GCA_021498435.1 Yes Nakaseomyces glabratus UHNDB9664 GCA 021498385.1 Yes Nakaseomyces glabratus Unknown SRR24010543 Yes 1Omitted due to poor genome assembly quality upon BLAST analysis

[0407] 2Reassigned to Candida parapsilosis based on BLAST analysis of assembled genome 3Reassigned to Candida albicans based on BLAST analysis of assembled genome

[0408] 4No BLAST hit generated against N. glabratus ATCC 2001 (GenBank ref: CP048127.1) Table 2

[0409] Amplifies with

[0410] P. kudriavzevii assay

[0411] Strain genome _ Accession no. in silico Comments Pichia kudriavzevii 1418 SRR18739952 No 1 Pichia kudriavzevii 1434 SRR18739963 No 1 Pichia kudriavzevii 1786 SRR18739962 No 2 Pichia kudriavzevii 20058T SRR18739960 No 3 Pichia kudriavzevii 706K SRR18739956 No 1 Pichia kudriavzevii MAG SRR18739950 No 2 Pichia kudriavzevii K_20_1 SRR19696123 Yes 4 Pichia kudriavzevii K_20_3 SRR19696121 Yes 4 Pichia kudriavzevii NRRL Y-10940 GCA_032595395.1 No 5 Ambrosiozyma (form. Pichia) ambrosiae NRRL Y-7524 GCA 003705185.2 No

[0412] Ambrosiozyma (form. Pichia) angophorae NRRL Y-7118 SRR16974435 No

[0413] Ambrosiozyma (form. Pichia) cicatricosa NRRL Y-17594T SRR16974258 No

[0414] Ambrosiozyma (form. Pichia crossotarsi) monospora JCM

[0415] 7599 GCA_001599995.1 No

[0416] Ambrosiozyma (form. Pichia crossotarsi) monospora (form.

[0417] P. crossotarsi) NBRC 1965 GCA 030267765.1 No

[0418] Ambrosiozyma (form. Pichia crossotarsi) monospora NRRLY- 1484 GCA_030579955.1 No

[0419] Ambrosiozyma (form. Pichia crossotarsi) monospora NRRL Y- 6732 GCA_030557395.1 No

[0420] Barnettozyma (form. Pichia) hawaiiensis NRRL Y-27270 SRR6476004 No

[0421] Barnettozyma (form. Pichia) populi NRRL Y-12728 GCA 003706705.3 No

[0422] Barnettozyma (form. Pichia) salicaria NRRL Y-6780 SRR6476007 No

[0423] Barnettozyma (form. Pichia) wickerhamii NRRL Y-2435 GCA_030574915.1 No

[0424] [Candida awuae] CBS 11011 GCA_030563725.1 No

[0425] [Candida] phayaonensis (form. Pichia) NBRC 108868 GCA_030558305.1 No

[0426] [Candida sylvatica] NRRL Y-7777 GCA_018344475.1 No

[0427] Debaryomycetaceae sp. (form. Pichia dianae) ZIM 2471 GCA 031081905.1 No

[0428] Hyphopichia heimii (form. Pichia) NRRLY-7502 SRR6475888 No

[0429] Komagataella (form. Pichia) pastoris ATCC 28485

[0430] Komagataella phaffii (form. Pichia pastoris) GS115 ERR1294016 No

[0431] Komagataella phaffii (form. Pichia pastoris) X-33 SRR8244566 No

[0432] Komagataella phaffii (form. Pichia pastoris) Y-11430 SRR8244569 No

[0433] Komagataella phaffii (form. Pichia pastoris) Y-12729 SRR8244568 No

[0434] Komagataella phaffii (form. Pichia pastoris) Y-17741 SRR8244572 No

[0435] Komagataella phaffii (form. Pichia pastoris) Y-48123 SRR8244565 No

[0436] Komagataella phaffii (form. Pichia pastoris) Y-48124 SRR8244563 No

[0437] Komagataella phaffii (form. Pichia pastoris) Y-7556 SRR8244570 No

[0438] Komagataella phaffii (form. Pichia pastoris) YB-378 SRR8244571 No

[0439] Komagataella phaffii (form. Pichia pastoris) YB-4289 SRR8244564 No

[0440] Komagataella phaffii (form. Pichia pastoris) YB-4290 SRR8244567 No

[0441] Millerozyma (form. Pichia) farinosa CBS 7064 ERR331065 No

[0442] Ogataea (form. Pichia) kodamae NRRL Y-17234 SRR6475947 No

[0443] Ogataea polymorpha (form. Pichia angusta)

[0444] NCYC_495_leul.l SRR3926512 No

[0445] Ogataea (form. Pichia) trehaloabstinens NRRL Y-27595 SRR6475949 No Phaffomyces (form. Pichia) antillensis NRRL Y-12881 SRR6475928 No Phaffomyces (form. Pichia) opuntiae NRRL Y-11707 SRR6475931 No Phaffomyces thermotolerans (form. Pichia opuntiae var.

[0446] thermotolerans) NRRL Y-11709 SRR6475929 No Pichia cabralensis CBS 11679 GCA_030569495.1 No Pichia cactophila CBS6926 GCA_039700745.1 No Pichia cactophila NRRL Y-10963 GCA_030563965.1 No Pichia californica 05-03-06 2 SRR4451748 No Pichia californica NRRL Y-27254 SRR16988878 No Pichia californica Olga-1 GCA_016584265.1 No Pichia californica Olga-2 GCA_016584195.1 No Pichia californica SB-116 SRR12998347 No Pichia californica SB-48 SRR12998349 No Pichia cephalocereana NRRL Y-17225 SRR16988745 No Pichia deserticola NRRL Y-12918 SRR16974570 No Pichia dianae ZIM_2471 SRR23868668 No Pichia eremophila NRRL Y-17224 SRR16974569 No Pichia ethanolica M2 GCA_001649435.1 No Pichia ethanolica NRRL Y-12615 SRR16974620 No Pichia exigua NRRL Y-10920 SRR6476036 No Pichia fermentans CDLB. YE03 GCA_035610515.1 No Pichia fermentans CLIB_258 ERR9707013 No Pichia fermentans CLIB_742 ERR9707004 No Pichia fermentans F124-03 GCA_037041205.1 No Pichia fermentans F5206 GCA_037041255.1 No Pichia fermentans fo / MP / 02 GCA_003339355.1 No Pichia fermentans KUST-G0103 GCA 030770065.1 No Pichia fermentans NCYC_562 GCA_029581925.1 No Pichia fermentans NRRL Y-1619 GCA_030570255.1 No Pichia fermentans PG124-03 GCA_037041515.1 No Pichia fermentans R5424 GCA_037041755.1 No Pichia gijzenarium CBS 15024 SRR16988796 No Pichia heedii NRRL Y- 10967 SRR6476037 No Pichia inconspicua 1282 SRR8506595 No Pichia inconspicua 14ANR23920 SRR8506591 No Pichia inconspicua 9_16 SRR8506590 No Pichia inconspicua CBS180 SRR8506592 No Pichia inconspicua Cll SRR8506589 No Pichia inconspicua CNM_CL6867 SRR8506594 No Pichia inconspicua IUM_96-0030 SRR8506593 No Pichia inconspicua LL867 SRR8506588 No Pichia inconspicua M1218 GCA_025531555.1 No Pichia inconspicua M126-08 GCA_037040195.1 No Pichia inconspicua NRRL Y-2029 SRR16989006 No Pichia inconspicua R1214 GCA_025531545.1 No Pichia inconspicua UCSC_1590 SRR8506597 No Pichia insulana CBS 11169 SRR16989225 No Pichia kluyveri 246 ERR10466323 No Pichia kluyveri APC 11.10 B GCA 030062975.1 No Pichia kluyveri B7036 GCA 023629335.1 No Pichia kluyveri CBA6002 GCA 003327685.1 No Pichia kluyveri CLIB_1225 ERR9707000 No Pichia kluyveri DX115-08 GCA_037040945.1 No Pichia kluyveri NCYC 246 GCA_947297755.1 No Pichia kluyveri NRRLY-11519 GCA_030564505.1 No Pichia kluyveri PK-24 GCA 037102705.1 No Pichia kluyveri UCDFST 05-608 GCA 029581865.1 No Pichia kluyveri X31-10 GCA_019096115.1 No Pichia manshurica B7015 GCA_025531645.1 No Pichia manshurica F8012 GCA_025531675.1 No Pichia manshurica KUST-F1705 GCA 030769885.1 No Pichia manshurica NRRL Y-27978 GCA 030220005.1 No Pichia manshurica NRRL Y-27978T SRR16988916 No Pichia manshurica P8010B GCA_025531675.1 No Pichia manshurica Y1106A GCA_025531695.1 No Pichia manshurica YM63 DRR1733476 No Pichia membranifaciens KS47-1 DRR063217 No Pichia membranifaciens NRRLY-2026 SRR427192 No Pichia nakasei CLIB_976 ERR9707006 No Pichia nakasei CLIB_1057 ERR9707005 No Pichia nakasei NRRL Y-7686 SRR6476039 No Pichia nanzhaoensis CBS 15346 SRR16988813 No Pichia norvegensis NRRL CBS 1922 GCA_003339405.1 No Pichia norvegensis NRRLY-7687 SRR16974589 No Pichia occidentalis ApC GCA_030142935.1 No Pichia occidentalis CLIB_3294 ERR9706983 No Pichia occidentalis F2013-T GCA 023629695.1 No Pichia occidentalis NRRL Y-6545 GCA_029581965.1 No Pichia occidentalis NRRL Y-7552 SRR6476041 No Pichia occidentalis NRRL YB-3389 GCA_029581915.1 No Pichia occidentalis PH4030B GCA_023629655.1 No Pichia occidentalis UCDFST_75-63 GCA 029581985.1 No Pichia paraexigua CBS 15237 SRR16988819 No Pichia pseudocactophila CBS6929 GCA 039700735.1 No Pichia pseudocactophila NRRLY-17239 SRR16974517 No Pichia pseudolambica NRRL Y-17318T SRR16989190 No Pichia rugopelliculosa NRRLY-17079 SRR16974621 No Pichia scutulata NRRLY-71663 GCA_030560845.1 No Pichia sp. F1605B GCA_025531535.1 No Pichia sp. KDB-2024a UFMG-CM-Y531 GCA_040437495.1 No Pichia sp. NRRL Y-12824 GCA_030560915.1 No Pichia sp. NRRL Y-12827 GCA_030570315.1 No Pichia sp. NRRL Y-12830 GCA_030564565.1 No Pichia sp. NRRL Y-27259 GCA 030563085.1 No Pichia sp. NRRL YB-4149 GCA_030556025.1 No Pichia sp. NRRL Y-27261 SRR16989226 No Pichia sp. VM-2024a CBS16747 GCA_040938015.1 No Pichia sp. yHKS168 SRR16974491 No Pichia sp. yHMH446 SRR16974481 No Pichia sp. P1605 GCA_025531595.1 No Pichia sp. P7020A GCA_025531525.1 No Pichia sp. PH4023 GCA_025531575.1 No Pichia sp. PX1907B GCA_025531625.1 No Pichia sporocuriosa NRRL Y-27347 GCA_030574275.1 No Pichia terricola 0621 SRR19224207 No Pichia terricola M2015 GCA 023629215.1 No Pichia terricola M2019 GCA 023629275.1 No Pichia terricola NRRL YB-4310 SRR6475882 No Pichia terricola P1002 GCA 023629295.1 No Pichia terricola P2008 GCA 023629235.1 No Pichia terricola PG8608 GCA_037041655.1 No Pichia terricola WJL-G4 GCA_022701355.1 No Pichia thaimueangensis NBRC 101967 GCA_030569635.1 No Saturnispora saitoi (form. Pichia) NRRL Y-6671 SRR6476045 No Yamadazyma nakazawae (form. Pichia) NRRL Y-7903 SRR6475911 No Yamadazyma philogaea (form. Pichia) NRRL Y-7813 SRR6475886 No Pichia kudriavzevii K_19_l SRR19696126 Yes Pichia kudriavzevii K_19_2 SRR19696125 Yes Pichia kudriavzevii K_20_2 SRR19696122 Yes Pichia kudriavzevii 1163J SRR18739957 Yes Pichia kudriavzevii 1173 SRR18739949 Yes Pichia kudriavzevii 1286 SRR18739955 Yes Pichia kudriavzevii 129 SRR5047288 Yes Pichia kudriavzevii 1372J SRR18739958 Yes Pichia kudriavzevii 1715 SRR18739953 Yes Pichia kudriavzevii 19 SRR18739951 Yes Pichia kudriavzevii 2018-F1-211 ERR4669761 Yes Pichia kudriavzevii 2018-F1-342 ERR4669763 Yes Pichia kudriavzevii 2018-F1-51 ERR4669796 Yes Pichia kudriavzevii 2018-F1-52 ERR4669748 Yes Pichia kudriavzevii 2018-F6-163 ERR4669785 Yes Pichia kudriavzevii 2018-F6-166 ERR4669788 Yes Pichia kudriavzevii 2018-F7-71 ERR4669792 Yes Pichia kudriavzevii 8110516017 ERR7438463 Yes Pichia kudriavzevii AC-490 SRR24010555 Yes Pichia kudriavzevii ASCUSDY21 GCA_019843505.1 Yes Pichia kudriavzevii ATCC SRR18739964 Yes Pichia kudriavzevii B036 SRR18739959 Yes Pichia kudriavzevii B114-03 GCA_037040875.1 Yes Pichia kudriavzevii B143 GCA_018105815.1 Yes Pichia kudriavzevii B67 SRR18739961 Yes Pichia kudriavzevii B7027 GCA_023629575.1 Yes Pichia kudriavzevii C-AR1 SRR6977192 Yes Pichia kudriavzevii CBS573 GCA_003054445.1 Yes Pichia kudriavzevii CBS5147 GCA_003054405.1 Yes Pichia kudriavzevii C-IT1 SRR6977184 Yes Pichia kudriavzevii Ckrusei653 SRR5638311 Yes Pichia kudriavzevii CLIB1212 ERR9706989 Yes Pichia kudriavzevii CLIB3242 ERR9706997 Yes Pichia kudriavzevii C-LK1 SRR6977179 Yes Pichia kudriavzevii CTeuk-1743 GCA_029290715.1 Yes Pichia kudriavzevii CY902 SRR8982851 Yes Pichia kudriavzevii D108-B21 GCA_023629435.1 Yes Pichia kudriavzevii E-GH1 SRR6977168 Yes Pichia kudriavzevii E-JP2 SRR6977173 Yes Pichia kudriavzevii E-JP3 SRR6977172 Yes Pichia kudriavzevii E-UK1 SRR6977174 Yes Pichia kudriavzevii E-WI1 SRR6977177 Yes Pichia kudriavzevii F2008 GCA_023629555.1 Yes Pichia kudriavzevii F2020 GCA 023629635.1 Yes Pichia kudriavzevii F4008B GCA_023629535.1 Yes Pichia kudriavzevii HJ2 GCA_030264835.1 Yes Pichia kudriavzevii IO_001 SRR10402504 Yes Pichia kudriavzevii IO_002 SRR10402503 Yes Pichia kudriavzevii IO_003 SRR10402505 Yes Pichia kudriavzevii IO_004 SRR10403089 Yes Pichia kudriavzevii IO_005 SRR10403306 Yes Pichia kudriavzevii IO_006 SRR10405243 Yes Pichia kudriavzevii IO_007 SRR10405280 Yes Pichia kudriavzevii IO_008 SRR10405281 Yes Pichia kudriavzevii IO_009 SRR10405248 Yes Pichia kudriavzevii IO_010 SRR10405284 Yes Pichia kudriavzevii IO_011 SRR10405288 Yes Pichia kudriavzevii IO_012 SRR10405289 Yes Pichia kudriavzevii IO_013 SRR10406141 Yes Pichia kudriavzevii IO_015 SRR10406146 Yes Pichia kudriavzevii IO_016 SRR10406152 Yes Pichia kudriavzevii IO_017 SRR10406155 Yes Pichia kudriavzevii IO_018 SRR10406144 Yes Pichia kudriavzevii IO_019 SRR10406150 Yes Pichia kudriavzevii IO_020 SRR10406145 Yes Pichia kudriavzevii IO_021 SRR10406154 Yes Pichia kudriavzevii IO_022 SRR10406153 Yes Pichia kudriavzevii IO_023 SRR10406143 Yes Pichia kudriavzevii IO_024 SRR10406151 Yes Pichia kudriavzevii IO_025 SRR10406142 Yes Pichia kudriavzevii IO_026 SRR10406156 Yes Pichia kudriavzevii IO_027 SRR10406184 Yes Pichia kudriavzevii IO_028 SRR10406183 Yes Pichia kudriavzevii IO_029 SRR10406182 Yes Pichia kudriavzevii IO_030 SRR10406157 Yes Pichia kudriavzevii IO_031 SRR10413694 Yes Pichia kudriavzevii IO_032 SRR10413692 Yes Pichia kudriavzevii IO_033 SRR10413717 Yes Pichia kudriavzevii IO_034 SRR10413690 Yes Pichia kudriavzevii IO_036 SRR10413689 Yes Pichia kudriavzevii IO_037 SRR10413916 Yes Pichia kudriavzevii IO_038 SRR10413716 Yes Pichia kudriavzevii IO_039 SRR10413691 Yes Pichia kudriavzevii IO_040 SRR10413693 Yes Pichia kudriavzevii IO_041 SRR10414461 Yes Pichia kudriavzevii IO_042 SRR10414468 Yes Pichia kudriavzevii IO_043 SRR10414466 Yes Pichia kudriavzevii IO_044 SRR10414465 Yes Pichia kudriavzevii IO_045 SRR10414467 Yes Pichia kudriavzevii IO_048 SRR10415356 Yes Pichia kudriavzevii IO_050 SRR10415359 Yes Pichia kudriavzevii IO_051 SRR10415357 Yes Pichia kudriavzevii IO_052 SRR10415354 Yes Pichia kudriavzevii IO_054 SRR10415358 Yes Pichia kudriavzevii IO_055 SRR10415355 Yes Pichia kudriavzevii IO143 SRR10846470 Yes Pichia kudriavzevii IO144 SRR10845531 Yes Pichia kudriavzevii IO145 SRR10849312 Yes Pichia kudriavzevii IO146 SRR10846617 Yes Pichia kudriavzevii IO147 SRR10904160 Yes Pichia kudriavzevii IO148 SRR10846469 Yes Pichia kudriavzevii IO149 SRR10849311 Yes Pichia kudriavzevii IO150 SRR10904153 Yes Pichia kudriavzevii IO151 SRR10846471 Yes Pichia kudriavzevii IO152 SRR10845523 Yes Pichia kudriavzevii IO153 SRR10846320 Yes Pichia kudriavzevii IO154 SRR10849313 Yes Pichia kudriavzevii IO155 SRR10846314 Yes Pichia kudriavzevii IO156 SRR10845555 Yes Pichia kudriavzevii IO157 SRR10846322 Yes Pichia kudriavzevii IO158 SRR10846323 Yes Pichia kudriavzevii IO159 SRR10846614 Yes Pichia kudriavzevii IO160 SRR10848433 Yes Pichia kudriavzevii K_19_3 SRR19696124 Yes Pichia kudriavzevii K_20_3 SRR19696121 Yes Pichia kudriavzevii KCKM 1001 GCA_021233995.1 Yes Pichia kudriavzevii KMBL5774 SRR5044099 Yes Pichia kudriavzevii M1110A GCA_023629315.1 Yes Pichia kudriavzevii M12 GCA 000286515.1 Yes Pichia kudriavzevii M2002 GCA_023629595.1 Yes Pichia kudriavzevii M7004B GCA 023629375.1 Yes Pichia kudriavzevii MCFLIP SRR18739954 Yes Pichia kudriavzevii NBRC1279 GCA_018402865.1 Yes Pichia kudriavzevii NBRC1664 GCA_018402885.1 Yes Pichia kudriavzevii NCYC 2658 GCA_029581905.1 Yes

[0447] Pichia kudriavzevii NG7 GCA 002798095.1 Yes

[0448] Pichia kudriavzevii NRRL YB-373 GCA_032595495.1 Yes

[0449] Pichia kudriavzevii NRRL YB-431 GCA_032595415.1 Yes

[0450] Pichia kudriavzevii NRRL YB-592 GCA_032595455.1 Yes

[0451] Pichia kudriavzevii NRRL YB-615 GCA_032595435.1 Yes

[0452] Pichia kudriavzevii NRRL Y-5396 SRR6476038 Yes

[0453] Pichia kudriavzevii Nuruk GCA_021014275.1 Yes

[0454] Pichia kudriavzevii P40-11 GCA_023629495.1 Yes

[0455] Pichia kudriavzevii P7006C GCA_023629475.1 Yes

[0456] Pichia kudriavzevii PB2809B GCA_025503585.1 Yes

[0457] Pichia kudriavzevii PM40-15 GCA_023629415.1 Yes

[0458] Pichia kudriavzevii S40-07 GCA_023629455.1 Yes

[0459] Pichia kudriavzevii SCHI0087. S.155 GCA_044592665.1 Yes

[0460] Pichia kudriavzevii SCHI0131. M.3 GCA_032359565.1 Yes

[0461] Pichia kudriavzevii SD108 GCA_000764455.1 Yes

[0462] Pichia kudriavzevii SJP GCA 003033855.1 Yes

[0463] Pichia kudriavzevii VPCI_1381-P-18 ERR7438469 Yes

[0464] Pichia kudriavzevii VPCI_1390-P-18 ERR7438470 Yes

[0465] Pichia kudriavzevii VPCI_1461-P-18 ERR7438471 Yes

[0466] Pichia kudriavzevii VPCI_1483-P-18 ERR7438466 Yes

[0467] Pichia kudriavzevii VPCI_1596-P-18 ERR7438467 Yes

[0468] Pichia kudriavzevii VPCI_1734-P-18 ERR7438468 Yes

[0469] Pichia kudriavzevii VPCI_494-P-18 ERR7438464 Yes

[0470] Pichia kudriavzevii VPCI_506-P-15 ERR7438465 Yes

[0471] Pichia kudriavzevii X2809A GCA_023629515.1 Yes

[0472] Pichia kudriavzevii Y114-04A GCA_037042275.1 Yes

[0473] Pichia kudriavzevii Y1104A GCA_023629615.1 Yes

[0474] Pichia kudriavzevii Y4012 GCA_023629355.1 Yes

[0475] Pichia kudriavzevii Y5A GCA_025503635.1 Yes

[0476] Pichia kudriavzevii YFJ002-604 ERR7438462 Yes Saturnispora (form. Pichia) saitoi NRRL Y-6671 SRR6476045 No Yamadazyma (form. Pichia) nakazawae NRRLY-7903 SRR6475911 No Yamadazyma (form. Pichia) philogaea NRRL Y-7813 SRR6475886 No

[0477] IReassigned to Candida parapsilosis based on BLAST analysis of assembled genome

[0478] 2Reassigned to Candida orthopsilosis based on BLAST analysis of assembled genome

[0479] 3Reassigned to Wickerhamomyces anomalus based on BLAST analysis of assembled genome

[0480] 4~50% reads have a SNP in the probe-binding region; non-fatal issue that would cause delayed amplification in this strain by ~1 PCR cycle

[0481] 5Truncated sequence at P. kudriavzevii amplicon site - suspect assembly issues (unfortunately no raw reads are available to redo the assembly) Table 3

[0482] Amplifies with Amplifies with C. albicans assay C. tropicalis assay Strain genome Accession no. in silico? in silico? Comments Candida albicans P102-02A1 GCA_037040415.1 No No 1 Candida africana CEC4854 GCA_019359905.1 Yes No 2 Candidozyma auris (form. Candida auris) Nevada PHL1 SRR23921795.1 Yes No 2 Nakaseomyces glabratus CG185 SRR12825233.1 Yes No 2 Nakaseomyces glabratus CG48b SRR12825218.1 Yes No 2 Nakaseomyces glabratus CG48h SRR12825216 Yes No 2 Nakaseomyces glabratus CAS14-5851 SRR8697329 Yes No 3 Nakaseomyces glabratus ISTA29 SRR13648885 Yes No 3 Candida dubliniensis CSPO SRR10977169 No Yes 4 Candida sp. LDI48194 GCA_001005365.1 No Yes 4 Candida sp. LN1 GCA_019280635.1 No Yes 4 Nakaseomyces glabratus PUJ-HUSI 916 SRR13441713 No Yes 5 Candida albicans CP-117-0 SRR14902727.1 No No 6 Candida albicans CP-117-P SRR14902726.1 No No 6 Candida albicans CP-121-C SRR14902725.1 No No 6 Candida albicans CP-121-D SRR14902724.1 No No 6 Candida albicans CP-121-E SRR14902723.1 No No 6 Candida albicans CP-121-F SRR14902722.1 No No 6 Candida albicans CP-121-G SRR14902721.1 No No 6 Candida albicans CP-121-H SRR14902719.1 No No 6 Candida albicans CP-121-1 SRR14902718.1 No No 6 Candida albicans CP-121-J SRR14902717.1 No No 6 Candida albicans CP-121-K SRR14902716.1 No No 6 Candida albicans CP-121-L SRR14902715.1 No No 6 Candida albicans CP-121-M SRR14902714.1 No No 6 Candida albicans CP-122-A SRR14902713.1 No No 6 Candida albicans CP-122-B SRR14902712.1 No No 6 [Candida] aaseri 2786 GCA_947297635.1 No No [Candida] awuae CBS 11011 GCA_030563725.1 No No [Candida] montana NRRLY-17326 GCA_003706615.3 No No [Candida] norvegica NRRLY-17660T GCA_030567455.1 No No [Candida] phayaonensis NBRC 108868 GCA_030558305.1 No No [Candida] pseudocylindracea CBS 10854T GCA_030558265.1 No No [Candida] qinlingensis CBS 9768 GCA_030563005.1 No No [Candida] sanyaensis CBS 12637 GCA_030562985.1 No No [Candida] sanyaensis UCD423 GCA_911254575.1 No No [Candida] sanyiensis CBS 10592 GCA_030569275.1 No No [Candida] silvatica NRRLY-7777 GCA_018344475.1 No No Ambrosiozyma kashinagicola (form. Candida kashinagacola) JCM 15019 GCA_001599075.1 No No Ambrosiozyma llanquihuensis (form. Candida llanquihuensis) NRRL Y-17657 GCA_030568425.1 No No Ambrosiozyma pseudovanderkliftii (form. Candida

[0483]

[0484] pseudovanderkliftii) NRRL Y-63632 GCA_003707895.3 No No Ambrosiozyma va nderkl iftii (form. C. vanderkliftii)

[0485] No No NRRL Y-63633 GCA 003705225.2

[0486] Candida albicans 101 GCA_041438225.1 Yes No Candida albicans 12C GCA_000773845.1 Yes No Candida albicans 19F GCA_000775445.1 Yes No Candida albicans 2018-F1-187 ERR4669755.1 Yes No Candida albicans 2018-F1-189 ERR4669758.1 Yes No Candida albicans 2018-F1-190 ERR4669759.1 Yes No Candida albicans 2018-F1-192 ERR4669760.1 Yes No Candida albicans 2018-F1-251 ERR4669762.1 Yes No Candida albicans 2018-F1-53 ERR4669797.1 Yes No Candida albicans 2018-F1-54 ERR4669749.1 Yes No Candida albicans 2018-F1-55 ERR4669750.1 Yes No Candida albicans 2018-F1-56 ERR4669798.1 Yes No Candida albicans 2018-F1-59 ERR4669751.1 Yes No Candida albicans 2018-F1-60 ERR4669752.1 Yes No Candida albicans 2018-F1-61 ERR4669753.1 Yes No Candida albicans 2018-F1-62 ERR4669754.2 Yes No Candida albicans 2018-F2-256 ERR4669764.1 Yes No Candida albicans 2018-F2-279 ERR4669765.1 Yes No Candida albicans 2018-F4-29 ERR4669767.1 Yes No Candida albicans 2018-F4-30 ERR4669768.1 Yes No Candida albicans 2018-F4-31 ERR4669769.1 Yes No Candida albicans 2018-F4-32 ERR4669770.1 Yes No Candida albicans 2018-F4-33 ERR4669771.1 Yes No Candida albicans 2018-F4-34 ERR4669772.1 Yes No Candida albicans 2018-F4-35 ERR4669773.1 Yes No Candida albicans 2018-F4-36 ERR4669774.2 Yes No Candida albicans 2018-F4-37 ERR4669775.1 Yes No Candida albicans 2018-F4-38 ERR4669776.1 Yes No Candida albicans 2018-F4-39 ERR4669777.1 Yes No Candida albicans 2018-F4-6 ERR4669766.1 Yes No Candida albicans 2018-F5-47 ERR4669781.1 Yes No Candida albicans 2018-F8-28 ERR4669793.1 Yes No Candida albicans 2018-F9-31 ERR4669794.1 Yes No Candida albicans 3153A GCA_000447595.1 Yes No Candida albicans A_18_1 SRR19696268.1 Yes No Candida albicans A_18_2 SRR19696137.1 Yes No Candida albicans A_18_3 SRR19696090.1 Yes No Candida albicans A_18_4 SRR19696079.1 Yes No Candida albicans A_18_5 SRR19696266.1 Yes No Candida albicans A_18_6 SRR19696255.1 Yes No Candida albicans A_18_7 SRR19696244.1 Yes No Candida albicans A_18_8 SRR19696233.1 Yes No Candida albicans A_18_9 SRR19696222.1 Yes No Candida albicans A_18_10 SRR19696267.1 Yes No Candida albicans A_18_11 SRR19696120.1 Yes No Candida albicans A_18_12 SRR19696109.1 Yes No Candida albicans A_18_13 SRR19696098.1 Yes No Candida albicans A_18_14 SRR19696159.1 Yes No Candida albicans A_18_15 SRR19696148.1 Yes No Candida albicans A_19_1 SRR19696211.1 Yes No Candida albicans A_19_2 SRR19696114.1 Yes No Candida albicans A_19_3 SRR19696113.2 Yes No Candida albicans A_19_4 SRR19696112.1 Yes No Candida albicans A_19_5 SRR19696111.1 Yes No Candida albicans A_19_6 SRR19696110.1 Yes No Candida albicans A_19_7 SRR19696108.1 Yes No Candida albicans A_19_8 SRR19696107.1 Yes No Candida albicans A_19_9 SRR19696106.1 Yes No Candida albicans A_19_10 SRR19696200.2 Yes No Candida albicans A_19_11 SRR19696189.1 Yes No Candida albicans A_19_12 SRR19696178.1 Yes No Candida albicans A_19_13 SRR19696167.1 Yes No Candida albicans A_19_14 SRR19696119.1 Yes No Candida albicans A_19_15 SRR19696118.1 Yes No Candida albicans A_19_16 SRR19696117.1 Yes No Candida albicans A_19_17 SRR19696116.1 Yes No Candida albicans A_19_18 SRR19696115.1 Yes No Candida albicans A_20_1 SRR19696105.1 Yes No Candida albicans A_20_2 SRR19696104.1 Yes No Candida albicans A_20_3 SRR19696103.1 Yes No Candida albicans A_20_4 SRR19696102.1 Yes No Candida albicans A_20_5 SRR19696101.1 Yes No Candida albicans A_20_6 SRR19696100.1 Yes No Candida albicans A_20_7 SRR19696099.1 Yes No Candida albicans A_21_1 SRR19696097.1 Yes No Candida albicans A_21_2 SRR19696096.1 Yes No Candida albicans A_21_3 SRR19696095.1 Yes No Candida albicans A20 GCA_000447575.1 Yes No Candida albicans A48 GCA_000447535.1 Yes No Candida albicans A67 GCA_000447515.1 Yes No Candida albicans A84 GCA_000447635.1 Yes No Candida albicans A92 GCA_000447475.1 Yes No Candida albicans A123 GCA_000447455.1 Yes No Candida albicans A155 GCA_000447615.1 Yes No Candida albicans A203 GCA_000447495.1 Yes No Candida albicans ade2_mutant-rep5 SRR18717080.1 Yes No Candida albicans ATCC 10231 GCA_002276455.1 Yes No Candida albicans ATCC 11651 GCA_015345795.1 Yes No Candida albicans ATCC 18804 GCA_015344825.1 Yes No Candida albicans ATCC 28121 GCA_015345685.1 Yes No Candida albicans ATCC 36802 GCA_029931725.1 Yes No Candida albicans ATCC 38289 GCA_015344895.1 Yes No Candida albicans ATCC 64124 GCA_015345625.1 Yes No Candida albicans ATCC 76485 GCA_015345605.1 Yes No Candida albicans ATCC 90029 GCA_015344855.1 Yes No Candida albicans ATCC 90819 GCA_015345785.1 Yes No Candida albicans ATCC 96901 GCA_015345665.1 Yes No Candida albicans ATCC MYA-1023 GCA_015345725.1 Yes No Candida albicans ATCC MYA-2876 GCA_015345745.1 Yes No Candida albicans ATCC MYA-427 GCA_015345835.1 Yes No Candida albicans ATCC MYA-574 GCA_015345815.1 Yes No Candida albicans BL007 GCA_029931695.1 Yes No Candida albicans BL077 GCA_029931675.1 Yes No Candida albicans BL152 GCA_029931665.1 Yes No Candida albicans BL167 GCA_029931635.1 Yes No Candida albicans BL266 GCA_029931615.1 Yes No Candida albicans BL273 GCA_029931595.1 Yes No Candida albicans BL288 GCA_029931585.1 Yes No Candida albicans BL296 GCA_029931525.1 Yes No Candida albicans BL300 GCA_029931495.1 Yes No Candida albicans BWP17 SRR26148731.1 Yes No Candida albicans CA-34-AB SRR14902704.2 Yes No Candida albicans CA-34-AC SRR14902703.1 Yes No Candida albicans CA-34-AD SRR14902720.1 Yes No Candida albicans CA-35-AF SRR14902711.1 Yes No Candida albicans CA-35-AG SRR14902710.1 Yes No Candida albicans CA-371-W SRR14902709.1 Yes No Candida albicans CA-371-X SRR14902708.1 Yes No Candida albicans CA-371-Y SRR14902707.1 Yes No Candida albicans CA-371-Z SRR14902706.1 Yes No Candida albicans CA-50-S SRR14902705.1 Yes No Candida albicans CA-50-T SRR14902702.1 Yes No Candida albicans Ca529L GCA 000691765.2 Yes No Candida albicans Ca6 GCA_000784695.1 Yes No Candida albicans CA-66-Q SRR14902701.1 Yes No Candida albicans CA-66-R SRR14902728.1 Yes No Candida albicans CA77 GCA_016906495.1 Yes No Candida albicans CHN1 GCA_017309835.1 Yes No Candida albicans D9-M2 GCA_023623395.1 Yes No Candida albicans F1308A GCA_025766345.1 Yes No Candida albicans F133-05 GCA_037041175.1 Yes No Candida albicans F2612A GCA_023623405.1 Yes No Candida albicans F4410 GCA_025766575.1 Yes No Candida albicans FDAARGOS_656 GCA_014049485.1 Yes No Candida albicans FLC-1 T1 GCA 028532805.1 Yes No Candida albicans FLC-1 T2 GCA 028532825.1 Yes No Candida albicans GC75 GCA_000773735.1 Yes No Candida albicans H892 GCA_011037055.1 Yes No Candida albicans HM1 GCA_029931505.1 Yes No Candida albicans JMN70 GCA_029931515.1 Yes No Candida albicans L26 GCA_000775455.1 Yes No Candida albicans M110-09 GCA_037039665.1 Yes No Candida albicans M6 GCA_025766415.1 Yes No Candida albicans NCYC 4144 GCA 005890765.1 No Candida albicans NCYC 4145 GCA 005890775.1 Yes No Candida albicans NCYC 4146 GCA_005890745.1 Yes No Candida albicans NCYC 597 ERR2708442.2 Yes No Candida albicans NRRL Y-12983 GCA_030582515.1 Yes No Candida albicans P132-04 GCA_037040515.1 Yes No Candida albicans P133-08 GCA_037040595.1 Yes No Candida albicans P34048 GCA_000775465.1 Yes No Candida albicans P37005 GCA_000773745.1 Yes No Candida albicans P37037 GCA 000773825.1 Yes No Candida albicans P37039 GCA_000784515.1 Yes No Candida albicans P57055 GCA_000775505.1 Yes No Candida albicans P57072 GCA 000773805.1 Yes No Candida albicans P60002 GCA_000784525.1 Yes No Candida albicans P75010 GCA_000784575.1 Yes No Candida albicans P75016 GCA_000784595.1 Yes No Candida albicans P75063 GCA_000775525.1 Yes No Candida albicans P76055 GCA_000784505.1 Yes No Candida albicans P76067 GCA_000784495.1 Yes No Candida albicans P78042 GCA_000784615.1 Yes No Candida albicans P78048 GCA 000773725.1 Yes No Candida albicans P87 GCA_000774085.1 Yes No Candida albicans P94015 GCA_000773755.1 Yes No Candida albicans PG4419B GCA_025766515.1 Yes No Candida albicans PYS4401 GCA_025767555.1 Yes No Candida albicans R1306 GCA_025766535.1 Yes No Candida albicans R1910 GCA_023623345.1 Yes No Candida albicans R2109 GCA_025766435.1 Yes No Candida albicans R2109-2 GCA_025766595.1 Yes No Candida albicans SC5314 GCA 032688725.1 Yes No Candida albicans SCHI0007. S.7 GCA_044592555.1 Yes No Candida albicans SCHI0007. S.8 GCA_044592615.1 Yes No Candida albicans SCHI0007. S.9 GCA_044592855.1 Yes No Candida albicans SCHI0076. S.3 GCA_044592765.1 Yes No Candida albicans SP_CRL_000G1 GCA_004026255.1 Yes No Candida albicans T1_Fluc_5 ERR6565669.1 Yes No Candida albicans T1_ND_5 ERR6565670.1 Yes No Candida albicans T1_NE_5 ERR6565671.1 Yes No Candida albicans TIMM 1768 GCA_003454735.1 Yes No Candida albicans UAB012-W3D5 GCA 002259805.1 Yes No Candida albicans UAB012-W7D4 GCA 002259875.1 Yes No Candida albicans UAB040-W3D3 GCA 002259885.1 Yes No Candida albicans UAB090-W2D7 GCA 002259865.1 Yes No Candida albicans UCSD GCA_014825715.1 Yes No Candida albicans WO-1 GCA_000149445.2 Yes No Candida albicans Y1318A GCA_025766445.1 Yes No Candida albicans Y1501A GCA_025766475.1 Yes No Candida blackwelliae CBS 10843 GCA_030579015.1 No No Candida bohioensis NRRL Y-27737 GCA_030582535.1 No No Candida buenavistaensis NRRL Y-27734 GCA_030582575.1 No No Candida chauliodis NRRL Y-27909 GCA_030557115.1 No No Candida coleopterorum CBS 14180 GCA_030565325.1 No No Candida corydali NRRLY-27910 GCA_003706475.3 No No Candida dubliniensis 107c_v4 SRR23500672 No No Candida dubliniensis 120 SRR23500782 No No Candida dubliniensis 122 SRR23500778 No No Candida dubliniensis 130 SRR23500625 No No Candida dubliniensis 134 SRR23500623 No No Candida dubliniensis 137c_v8 SRR23500689 No No Candida dubliniensis 138 SRR23500688 No No Candida dubliniensis 142 SRR23500681 No No Candida dubliniensis 156 SRR23500622 No No Candida dubliniensis 163 SRR23500621 No No Candida dubliniensis 164 SRR23500760 No No Candida dubliniensis 173 SRR23500619 No No Candida dubliniensis 174 SRR23500618 No No Candida dubliniensis 20 SRR23500682 No No Candida dubliniensis 2018-F4-40 ERR4669778 No No Candida dubliniensis 2018-F5-21 ERR4669780 No No Candida dubliniensis 2018-F6-161 ERR4669783 No No Candida dubliniensis 26 SRR23500718 No No Candida dubliniensis 26c_v8 SRR23500717 No No Candida dubliniensis 29 SRR23500716 No No Candida dubliniensis 34 SRR23500742 No No Candida dubliniensis 41 SRR23500643 No No Candida dubliniensis 56 SRR23500641 No No Candida dubliniensis 58 SRR23500640 No No Candida dubliniensis 60-13 SRR10977170 No No Candida dubliniensis 63 SRR23500594 No No Candida dubliniensis 67 SRR23500584 No No Candida dubliniensis 68 SRR23500543 No No Candida dubliniensis 7 SRR23500582 No No Candida dubliniensis 77 SRR23500532 No No Candida dubliniensis 82 SRR23500637 No No Candida dubliniensis 83 SRR23500529 No No Candida dubliniensis 85 SRR23500526 No No Candida dubliniensis 85c_v8 SRR23500519 No No Candida dubliniensis 86 SRR23500636 No No Candida dubliniensis 93 SRR23500518 No No Candida dubliniensis 94 SRR23500635 No No Candida dubliniensis CBS 7987 SRR25636127 No No Candida dubliniensis CD36 GCA_000026945.1 No No Candida dubliniensis D_18_1 SRR19696162 No No Candida dubliniensis D_20_1 SRR19696161 No No Candida dubliniensis D_20_2 SRR19696160 No No Candida dubliniensis D_21_1 SRR19696158 No No Candida dubliniensis NRRL Y-17841 GCA_030568755.1 No No Candida dubliniensis SC19-5644 SRR16959009 No No Candida dubliniensis Unknown SRR24010548 No No Candida dubliniensis UOB319 GCA_000647555.1 No No Candida dubliniensis UOB320 GCA_000647575.1 No No Candida frijolesensis NRRL Y-48060 GCA_030557105.1 No No Candida gigantensis NRRL Y-27736 GCA 030557085.1 No No Candida jiufengensis CBS 10846 GCA_024610255.1 No No Candida labiduridarum NRRLY-27940 GCA_030572435.1 No No Candida maltosa NRRL Y-17677 GCA 030582995.1 No No Candida maltosa Xu316 GCA_000344705.1 No No Candida margitis CBS 14175 GCA_024628925.1 No No Candida metapsilosis 2015_Cm 1 GCA_032715015.1 No No Candida metapsilosis 2015_Cm 2 GCA_032714915.1 No No Candida metapsilosis 2015_Cm 3 GCA_032714875.1 No No Candida metapsilosis 2015_Cm 4 GCA_032714855.1 No No Candida metapsilosis 414 GCA_019178775.1 No No Candida metapsilosis ATCC 96143 GCA_008904905.1 No No Candida metapsilosis BP57 GCA_017655625.1 No No Candida metapsilosis CANME GCA_900069165.1 No No Candida metapsilosis NRRL Y-48470 GCA 030582635.1 No No Candida morakotiae NRRL Y-48708 GCA_030564645.1 No No Candida neerlandica NRRLY-27057 GCA_030557875.1 No No Candida orthopsilosis AY2 GCA_000304155.1 No No Candida orthopsilosis Co 90-125 GCA 000315875.1 No No Candida orthopsilosis MCO456 GCA 900002835.2 No No Candida orthopsilosis NRRL Y-48468 GCA_030573215.1 No No Candida orthopsilosis P6004 GCA_025767575.1 No No Candida orthopsilosis SY36 GCA 026650225.1 No No Candida oxycetoniae CBS 10844 GCA_023343755.2 No No Candida parablackwelliae CBS 15228 GCA_030585045.1 No No Candida parachauliodes CBS 13928 GCA_030565145.1 No No Candida parapsilosis 2015_Cp GCA_032714795.1 No No Candida parapsilosis 2NR911 GCA 025767985.1 No No Candida parapsilosis 90-137 GCA_011316035.2 No No Candida parapsilosis ATCC 22019 GCA_903989565.1 No No Candida parapsilosis CBS 1954 GCA_900004165.1 No No Candida parapsilosis CBS 6318 GCA 000982555.2 No No Candida parapsilosis CBS 7157 GCA_963989715.1 No No Candida parapsilosis CDC317 GCA 036288975.1 No No Candida parapsilosis CLIB 214 GCA_903989565.1 No No Candida parapsilosis DE0235 GCA_008764215.1 No No Candida parapsilosis DX7810 GCA_037041095.1 No No Candida parapsilosis DX8911S GCA_037041125.1 No No Candida parapsilosis F3313A GCA 025767885.1 No No Candida parapsilosis FDAARGOS 650 GCA_014049585.1 No No Candida parapsilosis FDAARGOS 651 GCA_014049455.1 No No Candida parapsilosis FDAARGOS_652 GCA_014049445.1 No No Candida parapsilosis FDAARGOS_653 GCA_014049495.1 No No Candida parapsilosis GAI GCA 000982675.1 No No Candida parapsilosis HMC1 GCA 037952885.1 No No Candida parapsilosis M1113B GCA_025767915.1 No No Candida parapsilosis M6001A GCA_025767995.1 No No Candida parapsilosis M8011 GCA_025531885.1 No No Candida parapsilosis NCYC4289 GCA_947184175.1 No No Candida parapsilosis NCYC4418 GCA_964199955.1 No No Candida parapsilosis NRRLY-12969 GCA 030568775.1 No No Candida parapsilosis P7305 GCA_037040715.1 No No Candida parapsilosis PDH3311A-2 GCA_025767775.1 No No Candida parapsilosis PF3403 GCA 025767895.1 No No Candida parapsilosis PH3406 GCA_025767715.1 No No Candida parapsilosis PK4602 GCA 025768005.1 No No Candida parapsilosis PYS009A GCA_025768045.1 No No Candida parapsilosis R40-03 GCA_025767905.1 No No Candida parapsilosis SCHI0087. S.94 GCA_044592815.1 No No Candida parapsilosis SCHI0087. S.52 GCA_044589525.1 No No Candida parapsilosis SCHI0087. S.60 GCA_044589505.1 No No Candida parapsilosis SCHI0106. M.1 GCA_044592705.1 No No Candida parapsilosis SCHI0152. S.1 GCA_032357095.1 No No Candida parapsilosis SCHI0155. M.1 GCA_044592725.1 No No Candida parapsilosis USM026 GCA_016735825.1 No No Candida parapsilosis USM039K GCA_016735785.1 No No Candida parapsilosis VP_08 GCA_041430605.1 No No Candida parapsilosis Y4602 GCA 025767825.1 No No Candida pseudojiufengensis CBS 10847 GCA_024610245.1 No No Candida pseudoviswanathii (nom. inval.) CBS 13916 GCA_030565125.1 No No Candida sakaeoensis (nom. inval.) CBS 12318 GCA_030569255.1 No No Candida sojae GF41 GCA_001442715.1 No No Candida sojae NCYC-2607 GCA_013177575.1 No No Candida sojae NRRLY-17909 GCA_030582655.1 No No Candida sp. PB3310 GCA_023629255.1 No No Candida sp. Y128-05C GCA_037042315.1 No No Candida sp. Y128-05E GCA_037042355.1 No No Candida tetrigidarum NRRL Y-48142 GCA_030557055.1 No No Candida theae 20191146 GCA_013368535.1 No No Candida theae CBS 12239 GCA_024610275.1 No No Candida tropicalis 121 GCA 000633855.1 No Yes Candida tropicalis 2015_Ct 1 GCA_032714815.1 No Yes Candida tropicalis 2015_Ct 2 GCA_032714735.1 No Yes Candida tropicalis 2016_Ct 1 GCA_032714675.1 No Yes Candida tropicalis 2016_Ct 2 GCA_032714655.1 No Yes Candida tropicalis 2016_Ct 3 GCA_032714595.1 No Yes Candida tropicalis C4 GCA 021019025.2 No Yes Candida tropicalis CBW-2 GCA_024220415.1 No Yes Candida tropicalis F1002B GCA 025767625.1 No Yes Candida tropicalis IBUN-090-03567 GCA 019239295.1 No Yes Candida tropicalis M3302A GCA 025767605.1 No Yes Candida tropicalis MYA-3404 GCA_013177555.1 No Yes Candida tropicalis MYCT-0121-2021 GCA_032356845.1 No Yes Candida tropicalis MYCT-0618-2021 GCA 032356825.1 No Yes Candida tropicalis MYCT-0644-2021 GCA_032356485.1 No Yes Candida tropicalis MYCT-0816-2021 GCA_032356445.1 No Yes Candida tropicalis MYCT-0825-2021 GCA_035078395.1 No Yes Candida tropicalis MYCT-0997-2021 GCA_035078465.1 No Yes Candida tropicalis MYCT-1000-2021 GCA 032356805.1 No Yes Candida tropicalis MYCT-1211-2021 GCA 032356785.1 No Yes Candida tropicalis MYCT-1368-2021 GCA_032356765.1 No Yes Candida tropicalis MYCT-1405-2021 GCA 032356725.1 No Yes Candida tropicalis MYCT-1531-2021 GCA 032356705.1 No Yes Candida tropicalis MYCT-1551-2021 GCA 032356685.1 No Yes Candida tropicalis MYCT-1733-2021 GCA_032356745.1 No Yes Candida tropicalis MYCT-1862-2021 GCA_035078345.1 No Yes Candida tropicalis MYCT-1956-2021 GCA_032356665.1 No Yes Candida tropicalis MYCT-1957-2021 GCA_032356585.1 No Yes Candida tropicalis MYCT-2012-2020 GCA 032356865.1 No Yes Candida tropicalis MYCT-2043-2021 GCA_032356565.1 No Yes Candida tropicalis MYCT-2085-2021 GCA_032356605.1 No Yes Candida tropicalis MYCT-2184-2021 GCA_032356505.1 No Yes Candida tropicalis MYCT-2250-2020 GCA 032356885.1 No Yes Candida tropicalis MYCT-2423-2021 GCA_032356545.1 No Yes Candida tropicalis MYCT-2728-2021 GCA_032356465.1 No Yes Candida tropicalis MYCT-2752-2021 GCA 035078385.1 No Yes Candida tropicalis NRRL Y-12968 GCA_030582595.1 No Yes Candida tropicalis PF3311 GCA_025767665.1 No Yes Candida tropicalis PL3312 GCA 025767685.1 No Yes Candida tropicalis PNY GCA 023373895.1 No Yes Candida tropicalis SCHI0074. S.1 GCA_044592505.1 No Yes Candida tropicalis U1179 GCA 021018955.2 No Yes Candida tropicalis U1360 GCA 021018935.2 No Yes Candida tropicalis U873 GCA 021018975.2 No Yes Candida tropicalis Y1033A GCA 023623695.1 No Yes Candida tropicalis Y3313 GCA_023623415.1 No Yes Candida tropicalis Y6604 GCA_002864075.1 No Yes Candida tropicalis Z68 GCA_029582195.1 No Yes Candida tropicalis ZD-3 GCA_022984785.1 No Yes Candida verbasci CBS 12699 GCA_947670415.1 No No Candida viswanathii ATCC 20962 GCA 003327735.1 No No Candida viswanathii NRRL Y-6660 GCA 030566835.1 No No Candida xiaguanensis CBS 13923 GCA_030555925.1 No No Candidozyma auris (form. Candida auris) 12 GCA_027886145.1 No No Candidozyma auris (form. Candida auris) 15 GCA 027563775.1 No No Candidozyma auris (form. Candida auris) 16B25 GCA_947395165.1 No No Candidozyma auris (form. Candida auris) 20 GCA_027563635.1 No No Candidozyma auris (form. Candida auris) 20-1498 GCA_037074745.1 No No Candidozyma auris (form. Candida auris) 20-15 GCA_025430785.1 No No Candidozyma auris (form. Candida auris) 20-16 GCA_025430635.1 No No Candidozyma auris (form. Candida auris) 20-17 GCA_025430405.1 No No Candidozyma auris (form. Candida auris) 20-19 GCA_025430385.1 No No Candidozyma auris (form. Candida auris) 20-20 GCA_025430355.1 No No Candidozyma auris (form. Candida auris) 20-21 GCA_025430335.1 No No Candidozyma auris (form. Candida auris) 20-22 GCA_025430195.1 No No Candidozyma auris (form. Candida auris) 20-23 GCA_025429895.1 No No Candidozyma auris (form. Candida auris) 20-25 GCA_025429885.1 No No Candidozyma auris (form. Candida auris) 20-26 GCA_025429755.1 No No Candidozyma auris (form. Candida auris) 20-27 GCA_025429745.1 No No Candidozyma auris (form. Candida auris) 20-28 GCA_025429735.1 No No Candidozyma auris (form. Candida auris) 20-32 GCA_025429595.1 No No Candidozyma auris (form. Candida auris) 20-34 GCA_025429525.1 No No Candidozyma auris (form. Candida auris) 204 GCA_027563725.1 No No Candidozyma auris (form. Candida auris) 208 GCA_027563715.1 No No Candidozyma auris (form. Candida auris) 21 GCA_027563645.1 No No Candidozyma auris (form. Candida auris) 214 GCA 008729175.1 No No Candidozyma auris (form. Candida auris) 28 GCA_027563705.1 No No Candidozyma auris (form. Candida auris) 288 GCA 008729165.1 No No Candidozyma auris (form. Candida auris) 367 GCA 008729205.1 No No Candidozyma auris (form. Candida auris) 394-1 GCA 008729195.1 No No Candidozyma auris (form. Candida auris) 394-2 GCA 008729255.1 No No Candidozyma auris (form. Candida auris) 394-3 GCA 008729275.1 No No Candidozyma auris (form. Candida auris) 470140 GCA_031463115.1 No No Candidozyma auris (form. Candida auris) 6684 GCA_001189475.1 No No Candidozyma auris (form. Candida auris) Al GCA_014217455.1 No No Candidozyma auris (form. Candida auris) B11103 GCA_031359945.2 No No Candidozyma auris (form. Candida auris) B11205 GCA_016772135.1 No No Candidozyma auris (form. Candida auris) B11220 GCA 003013715.2 No No Candidozyma auris (form. Candida auris) B11221 GCA_031357565.2 No No Candidozyma auris (form. Candida auris) B11243 GCA_003014415.1 No No Candidozyma auris (form. Candida auris) B11244 GCA 031357835.2 No No Candidozyma auris (form. Candida auris) B11245 GCA_008275145.1 No No Candidozyma auris (form. Candida auris) B11809 GCA_016495685.1 No No Candidozyma auris (form. Candida auris) B12037 GCA_016772215.1 No No Candidozyma auris (form. Candida auris) B12043 GCA_016495645.1 No No Candidozyma auris (form. Candida auris) B12342 GCA_016772155.1 No No Candidozyma auris (form. Candida auris) B12631 GCA_016772195.1 No No Candidozyma auris (form. Candida auris) B13463 GCA_016495665.1 No No Candidozyma auris (form. Candida auris) B13916 GCA 016772235.1 No No Candidozyma auris (form. Candida auris) B17721 GCA_016772175.1 No No Candidozyma auris (form. Candida auris) B8441 GCA_002759435.3 No No Candidozyma auris (form. Candida auris) BA01 GCA 039852025.1 No No Candidozyma auris (form. Candida auris) BJCA001 GCA_018831645.1 No No Candidozyma auris (form. Candida auris) BJCA002 GCA 018902005.1 No No Candidozyma auris (form. Candida auris) BJCA003 GCA_024706545.1 No No Candidozyma auris (form. Candida auris) CA HTX1 GCA 009602605.1 No No Candidozyma auris (form. Candida auris) CA01 GCA 032888605.1 No No Candidozyma auris (form. Candida auris) CA1LBN GCA_019039595.1 No No Candidozyma auris (form. Candida auris) CA2LBN GCA_019039775.1 No No Candidozyma auris (form. Candida auris) CA4LBN GCA_019039735.1 No No Candidozyma auris (form. Candida auris) CA5LBN GCA_019039755.1 No No Candidozyma auris (form. Candida auris) CA6LBN GCA_019039715.1 No No Candidozyma auris (form. Candida auris) CA7LBN GCA_019332045.1 No No Candidozyma auris (form. Candida auris) CA8LBN GCA_019039635.1 No No Candidozyma auris (form. Candida auris) CA9LBN GCA_019039695.1 No No Candidozyma auris (form. Candida auris) CA10LBN GCA_019039615.1 No No Candidozyma auris (form. Candida auris) CA11LBN GCA_019039575.1 No No Candidozyma auris (form. Candida auris) CA12LBN GCA_019039675.1 No No Candidozyma auris (form. Candida auris) CA13LBN GCA_019039475.1 No No Candidozyma auris (form. Candida auris) CA14LBN GCA_019039515.1 No No Candidozyma auris (form. Candida auris) CA15LBN GCA_019039395.1 No No Candidozyma auris (form. Candida auris) CA16LBN GCA_019039655.1 No No Candidozyma auris (form. Candida auris) CA17LBN GCA_019039535.1 No No Candidozyma auris (form. Candida auris) CA18LBN GCA_019039555.1 No No Candidozyma auris (form. Candida auris) CA19LBN GCA_019039375.1 No No Candidozyma auris (form. Candida auris) CA20LBN GCA_019039295.1 No No Candidozyma auris (form. Candida auris) CA21LBN GCA_019039275.1 No No Candidozyma auris (form. Candida auris) CA22LBN GCA_019039435.1 No No Candidozyma auris (form. Candida auris) CA23LBN GCA_019039495.1 No No Candidozyma auris (form. Candida auris) CA24LBN GCA_019039455.1 No No Candidozyma auris (form. Candida auris) CA25LBN GCA_019039415.1 No No Candidozyma auris (form. Candida auris) CA26LBN GCA_019039355.1 No No Candidozyma auris (form. Candida auris) CA27LBN GCA_019039335.1 No No Candidozyma auris (form. Candida auris) CA28LBN GCA_019039315.1 No No Candidozyma auris (form. Candida auris) CA29LBN GCA_019039235.1 No No Candidozyma auris (form. Candida auris) CA197 GCA_035772375.1 No No Candidozyma auris (form. Candida auris) CA204 GCA_035772305.1 No No Candidozyma auris (form. Candida auris) CA205 GCA_035772365.1 No No Candidozyma auris (form. Candida auris) CA208 GCA_035772345.1 No No Candidozyma auris (form. Candida auris) CA210 GCA_035772265.1 No No Candidozyma auris (form. Candida auris) CA211 GCA_035772125.1 No No Candidozyma auris (form. Candida auris) CA212 GCA_035772165.1 No No Candidozyma auris (form. Candida auris) CA213 GCA_035772105.1 No No Candidozyma auris (form. Candida auris) CA217 GCA_035772185.1 No No Candidozyma auris (form. Candida auris) CA218 GCA_035772215.1 No No Candidozyma auris (form. Candida auris) CA219 GCA_035771935.1 No No Candidozyma auris (form. Candida auris) CA220 GCA_035771965.1 No No Candidozyma auris (form. Candida auris) CA221 GCA_035771895.1 No No Candidozyma auris (form. Candida auris) CA222 GCA 035771885.1 No No Candidozyma auris (form. Candida auris) CA224 GCA_035771925.1 No No Candidozyma auris (form. Candida auris) CA227 GCA_035771725.1 No No Candidozyma auris (form. Candida auris) CA229 GCA_035771705.1 No No Candidozyma auris (form. Candida auris) CA230 GCA_035771765.1 No No Candidozyma auris (form. Candida auris) CA231 GCA_035771735.1 No No Candidozyma auris (form. Candida auris) CA232 GCA_035771715.1 No No Candidozyma auris (form. Candida auris) CA-AM1 GCA_014673535.1 No No Candidozyma auris (form. Candida auris) cama02 GCA_032694445.1 No No Candidozyma auris (form. Candida auris) cama03 GCA_032694655.1 No No Candidozyma auris (form. Candida auris) cama04 GCA_032694695.1 No No Candidozyma auris (form. Candida auris) cama05 GCA_032694685.1 No No Candidozyma auris (form. Candida auris) cama06 GCA_032694745.1 No No Candidozyma auris (form. Candida auris) cama07 GCA_032694645.1 No No Candidozyma auris (form. Candida auris) cama08 GCA_032694485.1 No No Candidozyma auris (form. Candida auris) cama09 GCA_032694465.1 No No Candidozyma auris (form. Candida auris) cama10 GCA_032694475.1 No No Candidozyma auris (form. Candida auris) Case 1 GCA_035582645.1 No No Candidozyma auris (form. Candida auris) Case 2 GCA_035582625.1 No No Candidozyma auris (form. Candida auris) Case 3 GCA_035582605.1 No No Candidozyma auris (form. Candida auris) Case 4 GCA_035582585.1 No No Candidozyma auris (form. Candida auris) Case 5 GCA_035582555.1 No No Candidozyma auris (form. Candida auris) Case 6 GCA_035582525.1 No No Candidozyma auris (form. Candida auris) Case 7 GCA_035582505.1 No No Candidozyma auris (form. Candida auris) CAU924 / 6920 GCA_019968925.1 No No Candidozyma auris (form. Candida auris) CBS 10913 GCA_030581515.1 No No Candidozyma auris (form. Candida auris) F0083 GCA_032367535.1 No No Candidozyma auris (form. Candida auris) F1580 GCA_032714025.1 No No Candidozyma auris (form. Candida auris) F3485 GCA 032715285.1 No No Candidozyma auris (form. Candida auris) FG_GE10 GCA_014638765.1 No No Candidozyma auris (form. Candida auris) FG_GE2 GCA_014638955.1 No No Candidozyma auris (form. Candida auris) FG_GE3 GCA_014638965.1 No No Candidozyma auris (form. Candida auris) FG_GE4 GCA_014638895.1 No No Candidozyma auris (form. Candida auris) FG_GE5 GCA_014638885.1 No No Candidozyma auris (form. Candida auris) FG_GE6 GCA_014638875.1 No No Candidozyma auris (form. Candida auris) FG_GE7 GCA_014638825.1 No No Candidozyma auris (form. Candida auris) FG_GE8 GCA_014638805.1 No No Candidozyma auris (form. Candida auris) FG_GE9 GCA_014638775.1 No No Candidozyma auris (form. Candida auris) GZ0008 GCA_035220645.1 No No Candidozyma auris (form. Candida auris) 1-172 GCA_027563765.1 No No Candidozyma auris (form. Candida auris) IFRC2087 GCA_016809505.1 No No Candidozyma auris (form. Candida auris) JCM 15448 GCA 007168705.1 No No Candidozyma auris (form. Candida auris) L1537 / 2020 GCA 020809265.1 No No Candidozyma auris (form. Candida auris) LMDM 1219 GCA_041381755.1 No No Candidozyma auris (form. Candida auris) LOM GCA_005234155.1 No No Candidozyma auris (form. Candida auris) MRSN101498 GCA_029619135.1 No No Candidozyma auris (form. Candida auris) MRSN455051 GCA 029618695.1 No No Candidozyma auris (form. Candida auris) MRSN455060 GCA 029618685.1 No No Candidozyma auris (form. Candida auris) MRSN455079 GCA_029619145.1 No No Candidozyma auris (form. Candida auris) MRSN456999 GCA 029618675.1 No No Candidozyma auris (form. Candida auris) PL1 GCA_026743825.1 No No Candidozyma auris (form. Candida auris) PUSH159 GCA_041381095.1 No No Candidozyma auris (form. Candida auris) RCPF-1821 GCA_004287075.1 No No Candidozyma auris (form. Candida auris)

[0487] No SCHI0087. S.148 GCA_044589395.1 °

[0488] Candidozyma auris (form. Candida auris) SCO_240 GCA_041026505.1 No No Candidozyma auris (form. Candida auris) SCO_242 GCA_041026585.1 No No Candidozyma auris (form. Candida auris) SCO_248 GCA_041026605.1 No No Candidozyma auris (form. Candida auris) SCO_266 GCA_041026525.1 No No Candidozyma auris (form. Candida auris) SCO_267 GCA_041026465.1 No No Candidozyma auris (form. Candida auris) SCO_275 GCA_041026565.1 No No Candidozyma auris (form. Candida auris) SCO_276 GCA_041026545.1 No No Candidozyma auris (form. Candida auris) SCO_279 GCA_041026485.1 No No Candidozyma auris (form. Candida auris) VPCI

[0489] No 479 / P / 13 GCA_001049995.1 °

[0490] 1Genome has 4736 contigs - suspect assembly quality issues. Unfortunately no raw reads to verify

[0491] 2Reassigned to Candida albicans based on BLAST analysis of assembled genome

[0492] 3Genome is 22Mbp, which is ~10Mbp larger than the N. glabratus genome. Contains a substantial number of C. albicans contigs according to Kraken2, including our amplicon region

[0493] 4Reassigned to Candida tropicalis based on BLAST analysis of assembled genome

[0494] SGenome is 26Mbp, which is ~14Mbp larger than the N. glabratus genome. Contains a substantial number of C. tropicalis contigs according to BLAST, including our amplicon region

[0495] 6Reassigned to Candida parapsilosis based on BLAST analysis of assembled genome Table 4

[0496] Amplifies with C.

[0497] Amplifies with C. dubliniensis parapsilosis assay assay in Strain genome Accession no. in silico? silica? Comments Nakaseomyces glabratus G_21_4 SRR19696261 Yes No | Candida dubliniensis 68 SRR23500543 No Yes 2 Pichia kudriavzevii 1418 SRR18739952 Yes No 3 Pichia kudriavzevii 1434 SRR18739963 Yes No 3 Pichia kudriavzevii 706K SRR18739956 Yes No 3 Nakaseomyces glabratus CAS08-0629 SRR8697473.1 Yes No 3 [Candida] aaseri 2786 GCA_947297635.1 No No [Candida] awuae CBS 11011 GCA_030563725.1 No No [Candida] montana NRRL Y- 17326 GCA_003706615.3 No No [Candida] norvegica NRRL Y- 17660T GCA_030567455.1 No No [Candida] phayaonensis NBRC 108868 GCA_030558305.1 No No [Candida] pseudocylindracea CBS 10854T GCA_030558265.1 No No [Candida] qinlingensis CBS 9768 GCA_030563005.1 No No [Candida] sanyaensis CBS 12637 GCA_030562985.1 No No [Candida] sanyaensis UCD423 GCA_911254575.1 No No [Candida] sanyiensis CBS 10592 GCA_030569275.1 No No [Candida] silvatica NRRL Y-7777 GCA_018344475.1 No No Ambrosiozyma ambrosiae (form. P. ambrosiae) NRRLY- No No 7524 GCA_003705185.2 Ambrosiozyma angophorae (form. P. angophorae) NRRL Y- No No 7118 GCA_030567475.1 Ambrosiozyma cicatricosa (form. P. cicatricosa) NRRLY- No No 17594T GCA_030579935.1 Ambrosiozyma kamigamensis NRRL Y-63629 GCA_030572235.1 No No Ambrosiozyma kashinagicola (form. C. kashinagacola) JCM No No 15019 GCA_001599075.1 Ambrosiozyma llanquihuensis (form. C. Ilanquihuensis)

[0498] No No NRRL Y-17657 GCA_030568425.1 Ambrosiozyma maleeae NRRL Y-63635 GCA_003706635.2 No No Ambrosiozyma monospora (form. P. crossotarsi) JCM 7599 GCA_001599995.1 No No Ambrosiozyma monospora (form. P. crossotarsi) NBRC No No 1965 GCA_030267765.1 Ambrosiozyma monospora (form. P. crossotarsi) NRRL Y- No No 1484 GCA_030579955.1 Ambrosiozyma neoplatypodis NRRL Y-63630 GCA_030567495.1 No No Ambrosiozyma oregonensis NRRLY-6106 GCA_003706315.3 No No Ambrosiozyma philentoma NRRLY-7523 GCA_003707675.3 No No Ambrosiozyma platypodis (form. P. ambrosiae) NRRL Y- No No 6732 GCA_030557395.1 Ambrosiozyma pseudovanderkliftii (form. C.

[0499] No No pseudovanderkliftii) NRRLY-63632 GCA_003707895.3 Ambrosiozyma vanderkliftii (form. C. vanderkliftii) NRRL Y- No No 63633 GCA_003705225.2 Barnettozyma botsteinii 1118 GCA_020280145.1 No No Barnettozyma californica NRRLY-17395 GCA_003706655.3 No No Barnettozyma californica PB4207 GCA_023627795.1 No No Barnettozyma californica UCD09 GCA_003123585.1 No No

[0500]

[0501] Barnettozyma californica UCD89 GCA_003123885.1 No No Barnettozyma hawaiiensis (form. P. hawaiiensis) NRRL Y- No No 27270 GCA_003706665.2

[0502] Barnettozyma populi (form. P. populi) NRRL Y-12728 GCA_003706705.3 No No Barnettozyma pratensis NRRL Y-12696 GCA_003707865.2 No No Barnettozyma salicaria (form. P. salicaria) NRRL Y-6780 GCA_003707885.2 No No Barnettozyma siamensis CBS 13392 GCA_030579035.1 No No Barnettozyma sp. UFMG-CM-Y5996 GCA_030580455.1 No No Barnettozyma sucrosica CBS 11512 GCA_030558145.1 No No Barnettozyma vustinii CBS 11554 GCA_030569515.1 No No Barnettozyma wickerhamii (form. P. wickerhamii) NRRL Y- No No 2435 GCA_030574915.1

[0503] Candida africana CEC4854 GCA_019359905.1 No No Candida albicans 101 GCA_041438225.1 No No Candida albicans 12C GCA_000773845.1 No No Candida albicans 19F GCA_000775445.1 No No Candida albicans 2018-F1-187 ERR4669755.1 No No Candida albicans 2018-F1-189 ERR4669758.1 No No Candida albicans 2018-F1-190 ERR4669759.1 No No Candida albicans 2018-F1-192 ERR4669760.1 No No Candida albicans 2018-F1-251 ERR4669762.1 No No Candida albicans 2018-F1-53 ERR4669797.1 No No Candida albicans 2018-F1-54 ERR4669749.1 No No Candida albicans 2018-F1-55 ERR4669750.1 No No Candida albicans 2018-F1-56 ERR4669798.1 No No Candida albicans 2018-F1-59 ERR4669751.1 No No Candida albicans 2018-F1-60 ERR4669752.1 No No Candida albicans 2018-F1-61 ERR4669753.1 No No Candida albicans 2018-F1-62 ERR4669754.2 No No Candida albicans 2018-F2-256 ERR4669764.1 No No Candida albicans 2018-F2-279 ERR4669765.1 No No Candida albicans 2018-F4-29 ERR4669767.1 No No Candida albicans 2018-F4-30 ERR4669768.1 No No Candida albicans 2018-F4-31 ERR4669769.1 No No Candida albicans 2018-F4-32 ERR4669770.1 No No Candida albicans 2018-F4-33 ERR4669771.1 No No Candida albicans 2018-F4-34 ERR4669772.1 No No Candida albicans 2018-F4-35 ERR4669773.1 No No Candida albicans 2018-F4-36 ERR4669774.2 No No Candida albicans 2018-F4-37 ERR4669775.1 No No Candida albicans 2018-F4-38 ERR4669776.1 No No Candida albicans 2018-F4-39 ERR4669777.1 No No Candida albicans 2018-F4-6 ERR4669766.1 No No Candida albicans 2018-F5-47 ERR4669781.1 No No Candida albicans 2018-F8-28 ERR4669793.1 No No Candida albicans 2018-F9-31 ERR4669794.1 No No Candida albicans 3153A GCA_000447595.1 No No Candida albicans A_18_1 SRR19696268.1 No No Candida albicans A_18_10 SRR19696267.1 No No Candida albicans A_18_11 SRR19696120.1 No No Candida albicans A_18_12 SRR19696109.1 No No Candida albicans A_18_13 SRR19696098.1 No No Candida albicans A_18_14 SRR19696159.1 No No Candida albicans A_18_15 SRR19696148.1 No No Candida albicans A_18_2 SRR19696137.1 No No Candida albicans A_18_3 SRR19696090.1 No No Candida albicans A_18_4 SRR19696079.1 No No Candida albicans A_18_5 SRR19696266.1 No No Candida albicans A_18_6 SRR19696255.1 No No Candida albicans A_18_7 SRR19696244.1 No No Candida albicans A_18_8 SRR19696233.1 No No Candida albicans A_18_9 SRR19696222.1 No No Candida albicans A_19_1 SRR19696211.1 No No Candida albicans A_19_10 SRR19696200.2 No No Candida albicans A_19_11 SRR19696189.1 No No Candida albicans A_19_12 SRR19696178.1 No No Candida albicans A_19_13 SRR19696167.1 No No Candida albicans A_19_14 SRR19696119.1 No No Candida albicans A_19_15 SRR19696118.1 No No Candida albicans A_19_16 SRR19696117.1 No No Candida albicans A_19_17 SRR19696116.1 No No Candida albicans A_19_18 SRR19696115.1 No No Candida albicans A_19_2 SRR19696114.1 No No Candida albicans A_19_3 SRR19696113.2 No No Candida albicans A_19_4 SRR19696112.1 No No Candida albicans A_19_5 SRR19696111.1 No No Candida albicans A_19_6 SRR19696110.1 No No Candida albicans A_19_7 SRR19696108.1 No No Candida albicans A_19_8 SRR19696107.1 No No Candida albicans A_19_9 SRR19696106.1 No No Candida albicans A_20_1 SRR19696105.1 No No Candida albicans A_20_2 SRR19696104.1 No No Candida albicans A_20_3 SRR19696103.1 No No Candida albicans A_20_4 SRR19696102.1 No No Candida albicans A_20_5 SRR19696101.1 No No Candida albicans A_20_6 SRR19696100.1 No No Candida albicans A_20_7 SRR19696099.1 No No Candida albicans A_21_1 SRR19696097.1 No No Candida albicans A_21_2 SRR19696096.1 No No Candida albicans A_21_3 SRR19696095.1 No No Candida albicans A123 GCA_000447455.1 No No Candida albicans A155 GCA_000447615.1 No No Candida albicans A20 GCA_000447575.1 No No Candida albicans A203 GCA_000447495.1 No No Candida albicans A48 GCA_000447535.1 No No Candida albicans A67 GCA_000447515.1 No No Candida albicans A84 GCA_000447635.1 No No Candida albicans A92 GCA_000447475.1 No No Candida albicans ade2_mutant-rep5 SRR18717080.1 No No Candida albicans ATCC 10231 GCA_002276455.1 No No Candida albicans ATCC 11651 GCA_015345795.1 No No Candida albicans ATCC 18804 GCA_015344825.1 No No Candida albicans ATCC 28121 GCA_015345685.1 No No Candida albicans ATCC 36802 GCA_029931725.1 No No Candida albicans ATCC 38289 GCA_015344895.1 No No Candida albicans ATCC 64124 GCA_015345625.1 No No Candida albicans ATCC 76485 GCA_015345605.1 No No Candida albicans ATCC 90029 GCA_015344855.1 No No Candida albicans ATCC 90819 GCA_015345785.1 No No Candida albicans ATCC 96901 GCA_015345665.1 No No Candida albicans ATCC MYA-1023 GCA_015345725.1 No No Candida albicans ATCC MYA-2876 GCA_015345745.1 No No Candida albicans ATCC MYA-427 GCA_015345835.1 No No Candida albicans ATCC MYA-574 GCA_015345815.1 No No Candida albicans BL007 GCA_029931695.1 No No Candida albicans BL077 GCA_029931675.1 No No Candida albicans BL152 GCA_029931665.1 No No Candida albicans BL167 GCA_029931635.1 No No Candida albicans BL266 GCA_029931615.1 No No Candida albicans BL273 GCA_029931595.1 No No Candida albicans BL288 GCA_029931585.1 No No Candida albicans BL296 GCA_029931525.1 No No Candida albicans BL300 GCA_029931495.1 No No Candida albicans BWP17 SRR26148731.1 No No Candida albicans CA-34-AB SRR14902704.2 No No Candida albicans CA-34-AC SRR14902703.1 No No Candida albicans CA-34-AD SRR14902720.1 No No Candida albicans CA-35-AF SRR14902711.1 No No Candida albicans CA-35-AG SRR14902710.1 No No Candida albicans CA-371-W SRR14902709.1 No No Candida albicans CA-371-X SRR14902708.1 No No Candida albicans CA-371-Y SRR14902707.1 No No Candida albicans CA-371-Z SRR14902706.1 No No Candida albicans CA-50-S SRR14902705.1 No No Candida albicans CA-50-T SRR14902702.1 No No Candida albicans Ca529L GCA_000691765.2 No No Candida albicans Ca6 GCA_000784695.1 No No Candida albicans CA-66-Q SRR14902701.1 No No Candida albicans CA-66-R SRR14902728.1 No No Candida albicans CA77 GCA_016906495.1 No No Nakaseomyces glabratus CG185 SRR12825233.1 No No4Nakaseomyces glabratus CG48b SRR12825218.1 No No 4 Nakaseomyces glabratus CG84h SRR12825216.1 No No 4 Candida albicans CHN1 GCA_017309835.1 No No Candida albicans D9-M2 GCA_023623395.1 No No Candida albicans F1308A GCA_025766345.1 No No Candida albicans F133-05 GCA_037041175.1 No No Candida albicans F2612A GCA_023623405.1 No No Candida albicans F4410 GCA_025766575.1 No No Candida albicans FDAARGOS_656 GCA_014049485.1 No No Candida albicans FLC-1 T1 GCA_028532805.1 No No Candida albicans FLC-1 T2 GCA_028532825.1 No No Candida albicans GC75 GCA_000773735.1 No No Candida albicans H892 GCA_011037055.1 No No Candida albicans HM1 GCA_029931505.1 No No Candida albicans JMN70 GCA_029931515.1 No No Candida albicans L26 GCA_000775455.1 No No Candida albicans M110-09 GCA_037039665.1 No No Candida albicans M6 GCA_025766415.1 No No Candida albicans NCYC 4144 GCA_005890765.1 No No Candida albicans NCYC 4145 GCA_005890775.1 No No Candida albicans NCYC 4146 GCA_005890745.1 No No Candida albicans NCYC 597 ERR2708442.2 No No Candida albicans NRRL Y-12983 GCA_030582515.1 No No Candida albicans P102-02A1 GCA_037040415.1 No No Candida albicans P132-04 GCA_037040515.1 No No Candida albicans P133-08 GCA_037040595.1 No No Candida albicans P34048 GCA_000775465.1 No No Candida albicans P37005 GCA_000773745.1 No No Candida albicans P37037 GCA_000773825.1 No No Candida albicans P37039 GCA_000784515.1 No No Candida albicans P57055 GCA_000775505.1 No No Candida albicans P57072 GCA_000773805.1 No No Candida albicans P60002 GCA_000784525.1 No No Candida albicans P75010 GCA_000784575.1 No No Candida albicans P75016 GCA_000784595.1 No No Candida albicans P75063 GCA_000775525.1 No No Candida albicans P76055 GCA_000784505.1 No No Candida albicans P76067 GCA_000784495.1 No No Candida albicans P78042 GCA_000784615.1 No No Candida albicans P78048 GCA_000773725.1 No No Candida albicans P87 GCA_000774085.1 No No Candida albicans P94015 GCA_000773755.1 No No Candida albicans PG4419B GCA_025766515.1 No No Candida albicans PYS4401 GCA_025767555.1 No No Candida albicans R1306 GCA_025766535.1 No No Candida albicans R1910 GCA_023623345.1 No No Candida albicans R2109 GCA_025766435.1 No No Candida albicans R2109-2 GCA_025766595.1 No No Candida albicans SC5314 GCA_032688725.1 No No Candida albicans SCHI0007. S.7 GCA_044592555.1 No No Candida albicans SCHI0007. S.8 GCA_044592615.1 No No Candida albicans SCHI0007. S.9 GCA_044592855.1 No No Candida albicans SCHI0076. S.3 GCA_044592765.1 No No Candida albicans SP_CRL_000G1 GCA_004026255.1 No No Candida albicans T1_Fluc_5 ERR6565669.1 No No Candida albicans T1_ND_5 ERR6565670.1 No No Candida albicans T1_NE_5 ERR6565671.1 No No Candida albicans TIMM 1768 GCA_003454735.1 No No Candida albicans UAB012-W3D5 GCA_002259805.1 No No Candida albicans UAB012-W7D4 GCA_002259875.1 No No Candida albicans UAB040-W3D3 GCA_002259885.1 No No Candida albicans UAB090-W2D7 GCA_002259865.1 No No Candida albicans UCSD GCA_014825715.1 No No Candida albicans WO-1 GCA_000149445.2 No No Candida albicans Y1318A GCA_025766445.1 No No Candida albicans Y1501A GCA_025766475.1 No No Candida blackwelliae CBS 10843 GCA_030579015.1 No No Candida bohioensis NRRLY-27737 GCA_030582535.1 No No Candida buenavistaensis NRRLY-27734 GCA_030582575.1 No No Candida chauliodis NRRL Y- 27909 GCA_030557115.1 No No Candida coleopterorum CBS 14180 GCA_030565325.1 No No Candida corydali NRRL Y-27910 GCA_003706475.3 No No Candida dubliniensis 107c_v4 SRR23500672 No Yes Candida dubliniensis 120 SRR23500782 No Yes Candida dubliniensis 122 SRR23500778 No Yes Candida dubliniensis 130 SRR23500625 No Yes Candida dubliniensis 134 SRR23500623 No Yes Candida dubliniensis 137c_v8 SRR23500689 No Yes Candida dubliniensis 138 SRR23500688 No Yes Candida dubliniensis 142 SRR23500681 No Yes Candida dubliniensis 156 SRR23500622 No Yes Candida dubliniensis 163 SRR23500621 No Yes Candida dubliniensis 164 SRR23500760 No Yes Candida dubliniensis 173 SRR23500619 No Yes Candida dubliniensis 174 SRR23500618 No Yes Candida dubliniensis 20 SRR23500682 No Yes Candida dubliniensis 2018-F4-40 ERR4669778 No Yes Candida dubliniensis 2018-F5-21 ERR4669780 No Yes Candida dubliniensis 2018-F6-161 ERR4669783 No Yes Candida dubliniensis 26 SRR23500718 No Yes Candida dubliniensis 26c_v8 SRR23500717 No Yes Candida dubliniensis 29 SRR23500716 No Yes Candida dubliniensis 34 SRR23500742 No Yes Candida dubliniensis 41 SRR23500643 No Yes Candida dubliniensis 56 SRR23500641 No Yes Candida dubliniensis 58 SRR23500640 No Yes Candida dubliniensis 60-13 SRR10977170 No Yes Candida dubliniensis 63 SRR23500594 No Yes Candida dubliniensis 67 SRR23500584 No Yes Candida dubliniensis 7 SRR23500582 No Yes Candida dubliniensis 77 SRR23500532 No Yes Candida dubliniensis 82 SRR23500637 No Yes Candida dubliniensis 83 SRR23500529 No Yes Candida dubliniensis 85 SRR23500526 No Yes Candida dubliniensis 85c_v8 SRR23500519 No Yes Candida dubliniensis 86 SRR23500636 No Yes Candida dubliniensis 93 SRR23500518 No Yes Candida dubliniensis 94 SRR23500635 No Yes Candida dubliniensis CBS 7987 SRR25636127 No Yes Candida dubliniensis CD36 GCA_000026945.1 No Yes Candida dubliniensis D_18_1 SRR19696162 No Yes Candida dubliniensis D_20_1 SRR19696161 No Yes Candida dubliniensis D_20_2 SRR19696160 No Yes Candida dubliniensis D_21_1 SRR19696158 No Yes Candida dubliniensis NRRL Y-17841 GCA_030568755.1 No Yes Candida dubliniensis NRRL Y-17841 SRR16974338 No Yes Candida dubliniensis SC19-5644 SRR16959009 No Yes Candida dubliniensis Unknown SRR24010548 No Yes Candida dubliniensis UOB319 GCA_000647555.1 No Yes Candida dubliniensis UOB320 GCA_000647575.1 No Yes Candida frijolesensis NRRL Y-48060 GCA_030557105.1 No No Candida gigantensis NRRLY-27736 GCA_030557085.1 No No Candida jiufengensis CBS 10846 GCA_024610255.1 No No Candida labiduridarum NRRL Y-27940 GCA_030572435.1 No No Candida maltosa NRRL Y-17677 GCA_030582995.1 No No Candida maltosa Xu316 GCA_000344705.1 No No Candida margitis CBS 14175 GCA_024628925.1 No No Candida metapsilosis 2015_Cm 1 GCA_032715015.1 No No Candida metapsilosis 2015_Cm 2 GCA_032714915.1 No No Candida metapsilosis 2015_Cm 3 GCA_032714875.1 No No Candida metapsilosis 2015_Cm 4 GCA_032714855.1 No No Candida metapsilosis 414 GCA_019178775.1 No No Candida metapsilosis ATCC 96143 GCA_008904905.1 No No Candida metapsilosis BP57 GCA_017655625.1 No No Candida metapsilosis CANME GCA_900069165.1 No No Candida metapsilosis NRRL Y-48470 GCA_030582635.1 No No Candida morakotiae NRRLY-48708 GCA_030564645.1 No No Candida neerlandica NRRL Y-27057 GCA_030557875.1 No No Pichia kudriavzevii 1786 SRR18739962 No No Candida orthopsilosis AY2 GCA_000304155.1 No No Candida orthopsilosis Co 90-125 GCA_000315875.1 No No Pichia kudriavzevii MAG SRR18739950 No No Candida orthopsilosis MCO456 GCA_900002835.2 No No Candida orthopsilosis NRRLY-48468 GCA_030573215.1 No No Candida orthopsilosis P6004 GCA_025767575.1 No No Candida orthopsilosis SY36 GCA_026650225.1 No No Candida oxycetoniae CBS 10844 GCA_023343755.2 No No Candida parablackwelliae CBS 15228 GCA_030585045.1 No No Candida parachauliodes CBS 13928 GCA_030565145.1 No No Candida parapsilosis 2015_Cp GCA_032714795.1 Yes No Candida parapsilosis 2NR911 GCA_025767985.1 Yes No Candida parapsilosis 90-137 GCA_011316035.2 Yes No Candida parapsilosis ATCC 22019 GCA_903989565.1 Yes No Candida parapsilosis CBS 1954 GCA_900004165.1 Yes No Candida parapsilosis CBS 6318 GCA_000982555.2 Yes No Candida parapsilosis CBS 7157 GCA_963989715.1 Yes No Candida parapsilosis CDC317 GCA_036288975.1 Yes No Candida parapsilosis CLIB 214 GCA_903989565.1 Yes No Candida parapsilosis CP-117-O SRR14902727.1 Yes No6Candida parapsilosis CP-117-P SRR14902726.1 Yes No6Candida parapsilosis CP-121-C SRR14902725.1 Yes No6Candida parapsilosis CP-121-D SRR14902724.1 Yes No6Candida parapsilosis CP-121-E SRR14902723.1 Yes No6Candida parapsilosis CP-121-F SRR14902722.1 Yes No6Candida parapsilosis CP-121-G SRR14902721.1 Yes No6Candida parapsilosis CP-121-H SRR14902719.1 Yes No6Candida parapsilosis CP-121-1 SRR14902718.1 Yes No6Candida parapsilosis CP-121-J SRR14902717.1 Yes No6Candida parapsilosis CP-121-K SRR14902716.1 Yes No6Candida parapsilosis CP-121-L SRR14902715.1 Yes No6Candida parapsilosis CP-121-M SRR14902714.1 Yes No6Candida parapsilosis CP-122-A SRR14902713.1 Yes No6Candida parapsilosis CP-122-B SRR14902712.1 Yes No6Candida parapsilosis DE0235 GCA_008764215.1 Yes No Candida parapsilosis DX7810 GCA_037041095.1 Yes No Candida parapsilosis DX8911S GCA_037041125.1 Yes No Candida parapsilosis F3313A GCA_025767885.1 Yes No Candida parapsilosis FDAARGOS_650 GCA_014049585.1 Yes No Candida parapsilosis FDAARGOS_651 GCA_014049455.1 Yes No Candida parapsilosis FDAARGOS_652 GCA_014049445.1 Yes No Candida parapsilosis FDAARGOS_653 GCA_014049495.1 Yes No Candida parapsilosis GAI GCA_000982675.1 Yes No Candida parapsilosis HMC1 GCA_037952885.1 Yes No Candida parapsilosis M1113B GCA_025767915.1 Yes No Candida parapsilosis M6001A GCA_025767995.1 Yes No Candida parapsilosis M8011 GCA_025531885.1 Yes No Candida parapsilosis NCYC 4289 GCA_947184175.1 Yes No Candida parapsilosis NCYC 4418 GCA_964199955.1 Yes No Candida parapsilosis NRRL Y-12969 GCA_030568775.1 Yes No Candida parapsilosis P7305 GCA_037040715.1 Yes No Candida parapsilosis PDH3311A-2 GCA_025767775.1 Yes No Candida parapsilosis PF3403 GCA_025767895.1 Yes No Candida parapsilosis PH3406 GCA_025767715.1 Yes No Candida parapsilosis PK4602 GCA_025768005.1 Yes No Candida parapsilosis PYS009A GCA_025768045.1 Yes No Candida parapsilosis R40-03 GCA_025767905.1 Yes No Candida parapsilosis SCHI0087. S.94 GCA_044592815.1 No No Candida parapsilosis SCHI0087. S.52 GCA_044589525.1 No No Candida parapsilosis SCHI0087. S.60 GCA_044589505.1 No No Candida parapsilosis SCHI0106. M.1 GCA_044592705.1 No No Candida parapsilosis SCHI0152. S.1 GCA_032357095.1 No No Candida parapsilosis SCHI0155. M.1 GCA_044592725.1 No No Candida parapsilosis USM026 GCA_016735825.1 Yes No Candida parapsilosis USM039K GCA_016735785.1 Yes No Candida parapsilosis VP_08 GCA_041430605.1 Yes No Candida parapsilosis Y4602 GCA_025767825.1 Yes No Candida pseudojiufengensis CBS 10847 GCA_024610245.1 No No Candida pseudoviswanathii (nom. inval.) CBS 13916 GCA_030565125.1 No No Candida sakaeoensis (nom. inval.) CBS 12318 GCA_030569255.1 No No Candida sojae GF41 GCA_001442715.1 No No Candida sojae NCYC-2607 GCA_013177575.1 No No Candida sojae NRRL Y-17909 GCA_030582655.1 No No Candida sp. LDI48194 GCA_001005365.1 No No Candida sp. LN1 GCA_019280635.1 No No Candida sp. PB3310 GCA_023629255.1 No No Candida sp. Y128-05C GCA_037042315.1 No No Candida sp. Y128-05E GCA_037042355.1 No No Candida tetrigidarum NRRLY-48142 GCA_030557055.1 No No Candida theae 20191146 GCA_013368535.1 No No Candida theae CBS 12239 GCA_024610275.1 No No Candida tropicalis 121 GCA_000633855.1 No No Candida tropicalis 2015_Ct 1 GCA_032714815.1 No No Candida tropicalis 2015_Ct 2 GCA_032714735.1 No No Candida tropicalis 2016_Ct 1 GCA_032714675.1 No No Candida tropicalis 2016_Ct 2 GCA_032714655.1 No No Candida tropicalis 2016_Ct 3 GCA_032714595.1 No No Candida tropicalis C4 GCA_021019025.2 No No Candida tropicalis CBW-2 GCA_024220415.1 No No Candida tropicalis CSPO SRR10977169 No No 7 Candida tropicalis F1002B GCA_025767625.1 No No Candida tropicalis IBUN-090-03567 GCA_019239295.1 No No Candida tropicalis M3302A GCA_025767605.1 No No Candida tropicalis MYA-3404 GCA_013177555.1 No No Candida tropicalis MYCT-0121-2021 GCA_032356845.1 No No Candida tropicalis MYCT-0618-2021 GCA_032356825.1 No No Candida tropicalis MYCT-0644-2021 GCA_032356485.1 No No Candida tropicalis MYCT-0816-2021 GCA_032356445.1 No No Candida tropicalis MYCT-0825-2021 GCA_035078395.1 No No Candida tropicalis MYCT-0997-2021 GCA_035078465.1 No No Candida tropicalis MYCT-1000-2021 GCA_032356805.1 No No Candida tropicalis MYCT-1211-2021 GCA_032356785.1 No No Candida tropicalis MYCT-1368-2021 GCA_032356765.1 No No Candida tropicalis MYCT-1405-2021 GCA_032356725.1 No No Candida tropicalis MYCT-1531-2021 GCA_032356705.1 No No Candida tropicalis MYCT-1551-2021 GCA_032356685.1 No No Candida tropicalis MYCT-1733-2021 GCA_032356745.1 No No Candida tropicalis MYCT-1862-2021 GCA_035078345.1 No No Candida tropicalis MYCT-1956-2021 GCA_032356665.1 No No Candida tropicalis MYCT-1957-2021 GCA_032356585.1 No No Candida tropicalis MYCT-2012-2020 GCA_032356865.1 No No Candida tropicalis MYCT-2043-2021 GCA_032356565.1 No No Candida tropicalis MYCT-2085-2021 GCA_032356605.1 No No Candida tropicalis MYCT-2184-2021 GCA_032356505.1 No No Candida tropicalis MYCT-2250-2020 GCA_032356885.1 No No Candida tropicalis MYCT-2423-2021 GCA_032356545.1 No No Candida tropicalis MYCT-2728-2021 GCA_032356465.1 No No Candida tropicalis MYCT-2752-2021 GCA_035078385.1 No No Candida tropicalis NRRLY-12968 GCA_030582595.1 No No Candida tropicalis PF3311 GCA_025767665.1 No No Candida tropicalis PL3312 GCA_025767685.1 No No Candida tropicalis PNY GCA_023373895.1 No No Candida tropicalis SCHI0074. S.1 GCA_044592505.1 No Yes Candida tropicalis U1179 GCA_021018955.2 No No Candida tropicalis U1360 GCA_021018935.2 No No Candida tropicalis U873 GCA_021018975.2 No No Candida tropicalis Y1033A GCA_023623695.1 No No Candida tropicalis Y3313 GCA_023623415.1 No No Candida tropicalis Y6604 GCA_002864075.1 No No Candida tropicalis Z68 GCA_029582195.1 No No Candida tropicalis ZD-3 GCA_022984785.1 No No Candida verbasci CBS 12699 GCA_947670415.1 No No Candida viswanathii ATCC 20962 GCA_003327735.1 No No Candida viswanathii NRRL Y-6660 GCA_030566835.1 No No Candida xiaguanensis CBS 13923 GCA_030555925.1 No No Candidozyma auris (form. Candida auris) Nevada PHL1 SRR23921795.1 No No Candidozyma auris (form. Candida auris) SCHI0087. S.148 GCA_044589395.1 No No Candidozyma auris (form. Candida auris) 12 GCA_027886145.1 No No Candidozyma auris (form. Candida auris) 15 GCA_027563775.1 No No Candidozyma auris (form. Candida auris) 16B25 GCA_947395165.1 No No Candidozyma auris (form. Candida auris) 20 GCA_027563635.1 No No Candidozyma auris (form. Candida auris) 20-1498 GCA_037074745.1 No No Candidozyma auris (form. Candida auris) 20-15 GCA_025430785.1 No No Candidozyma auris (form. Candida auris) 20-16 GCA_025430635.1 No No Candidozyma auris (form. Candida auris) 20-17 GCA_025430405.1 No No Candidozyma auris (form. Candida auris) 20-19 GCA_025430385.1 No No Candidozyma auris (form. Candida auris) 20-20 GCA_025430355.1 No No Candidozyma auris (form. Candida auris) 20-21 GCA_025430335.1 No No Candidozyma auris (form. Candida auris) 20-22 GCA_025430195.1 No No Candidozyma auris (form. Candida auris) 20-23 GCA_025429895.1 No No Candidozyma auris (form. Candida auris) 20-25 GCA_025429885.1 No No Candidozyma auris (form. Candida auris) 20-26 GCA_025429755.1 No No Candidozyma auris (form. Candida auris) 20-27 GCA_025429745.1 No No Candidozyma auris (form. Candida auris) 20-28 GCA_025429735.1 No No Candidozyma auris (form. Candida auris) 20-32 GCA_025429595.1 No No Candidozyma auris (form. Candida auris) 20-34 GCA_025429525.1 No No Candidozyma auris (form. Candida auris) 204 GCA_027563725.1 No No Candidozyma auris (form. Candida auris) 208 GCA_027563715.1 No No Candidozyma auris (form. Candida auris) 21 GCA_027563645.1 No No Candidozyma auris (form. Candida auris) 214 GCA_008729175.1 No No Candidozyma auris (form. Candida auris) 28 GCA_027563705.1 No No Candidozyma auris (form. Candida auris) 288 GCA_008729165.1 No No Candidozyma auris (form. Candida auris) 367 GCA_008729205.1 No No Candidozyma auris (form. Candida auris) 394-1 GCA_008729195.1 No No Candidozyma auris (form. Candida auris) 394-2 GCA_008729255.1 No No Candidozyma auris (form. Candida auris) 394-3 GCA_008729275.1 No No Candidozyma auris (form. Candida auris) 470140 GCA_031463115.1 No No Candidozyma auris (form. Candida auris) 6684 GCA_001189475.1 No No Candidozyma auris (form. Candida auris) Al GCA_014217455.1 No No Candidozyma auris (form. Candida auris) B11103 GCA_031359945.2 No No Candidozyma auris (form. Candida auris) B11205 GCA_016772135.1 No No Candidozyma auris (form. Candida auris) B11220 GCA_003013715.2 No No Candidozyma auris (form. Candida auris) B11221 GCA_031357565.2 No No Candidozyma auris (form. Candida auris) B11243 GCA_003014415.1 No No Candidozyma auris (form. Candida auris) B11244 GCA_031357835.2 No No Candidozyma auris (form. Candida auris) B11245 GCA_008275145.1 No No Candidozyma auris (form. Candida auris) B11809 GCA_016495685.1 No No Candidozyma auris (form. Candida auris) B12037 GCA_016772215.1 No No Candidozyma auris (form. Candida auris) B12043 GCA_016495645.1 No No Candidozyma auris (form. Candida auris) B12342 GCA_016772155.1 No No Candidozyma auris (form. Candida auris) B12631 GCA_016772195.1 No No Candidozyma auris (form. Candida auris) B13463 GCA_016495665.1 No No Candidozyma auris (form. Candida auris) B13916 GCA_016772235.1 No No Candidozyma auris (form. Candida auris) B17721 GCA_016772175.1 No No Candidozyma auris (form. Candida auris) B8441 GCA_002759435.3 No No Candidozyma auris (form. Candida auris) BA01 GCA_039852025.1 No No Candidozyma auris (form. Candida auris) BJCA001 GCA_018831645.1 No No Candidozyma auris (form. Candida auris) BJCA002 GCA_018902005.1 No No Candidozyma auris (form. Candida auris) BJCA003 GCA_024706545.1 No No Candidozyma auris (form. Candida auris) CA HTX1 GCA_009602605.1 No No Candidozyma auris (form. Candida auris) CA01 GCA_032888605.1 No No Candidozyma auris (form. Candida auris) CA10LBN GCA_019039615.1 No No Candidozyma auris (form. Candida auris) CA11LBN GCA_019039575.1 No No Candidozyma auris (form. Candida auris) CA12LBN GCA_019039675.1 No No Candidozyma auris (form. Candida auris) CA13LBN GCA_019039475.1 No No Candidozyma auris (form. Candida auris) CA14LBN GCA_019039515.1 No No Candidozyma auris (form. Candida auris) CA15LBN GCA_019039395.1 No No Candidozyma auris (form. Candida auris) CA16LBN GCA_019039655.1 No No Candidozyma auris (form. Candida auris) CA17LBN GCA_019039535.1 No No Candidozyma auris (form. Candida auris) CA18LBN GCA_019039555.1 No No Candidozyma auris (form. Candida auris) CA197 GCA_035772375.1 No No Candidozyma auris (form. Candida auris) CA19LBN GCA_019039375.1 No No Candidozyma auris (form. Candida auris) CA1LBN GCA_019039595.1 No No Candidozyma auris (form. Candida auris) CA204 GCA_035772305.1 No No Candidozyma auris (form. Candida auris) CA205 GCA_035772365.1 No No Candidozyma auris (form. Candida auris) CA208 GCA_035772345.1 No No Candidozyma auris (form. Candida auris) CA20LBN GCA_019039295.1 No No Candidozyma auris (form. Candida auris) CA210 GCA_035772265.1 No No Candidozyma auris (form. Candida auris) CA211 GCA_035772125.1 No No Candidozyma auris (form. Candida auris) CA212 GCA_035772165.1 No No Candidozyma auris (form. Candida auris) CA213 GCA_035772105.1 No No Candidozyma auris (form. Candida auris) CA217 GCA_035772185.1 No No Candidozyma auris (form. Candida auris) CA218 GCA_035772215.1 No No Candidozyma auris (form. Candida auris) CA219 GCA_035771935.1 No No Candidozyma auris (form. Candida auris) CA21LBN GCA_019039275.1 No No Candidozyma auris (form. Candida auris) CA220 GCA_035771965.1 No No Candidozyma auris (form. Candida auris) CA221 GCA_035771895.1 No No Candidozyma auris (form. Candida auris) CA222 GCA_035771885.1 No No Candidozyma auris (form. Candida auris) CA224 GCA_035771925.1 No No Candidozyma auris (form. Candida auris) CA227 GCA_035771725.1 No No Candidozyma auris (form. Candida auris) CA229 GCA_035771705.1 No No Candidozyma auris (form. Candida auris) CA22LBN GCA_019039435.1 No No Candidozyma auris (form. Candida auris) CA230 GCA_035771765.1 No No Candidozyma auris (form. Candida auris) CA231 GCA_035771735.1 No No Candidozyma auris (form. Candida auris) CA232 GCA_035771715.1 No No Candidozyma auris (form. Candida auris) CA23LBN GCA_019039495.1 No No Candidozyma auris (form. Candida auris) CA24LBN GCA_019039455.1 No No Candidozyma auris (form. Candida auris) CA25LBN GCA_019039415.1 No No Candidozyma auris (form. Candida auris) CA26LBN GCA_019039355.1 No No Candidozyma auris (form. Candida auris) CA27LBN GCA_019039335.1 No No Candidozyma auris (form. Candida auris) CA28LBN GCA_019039315.1 No No Candidozyma auris (form. Candida auris) CA29LBN GCA_019039235.1 No No Candidozyma auris (form. Candida auris) CA2LBN GCA_019039775.1 No No Candidozyma auris (form. Candida auris) CA4LBN GCA_019039735.1 No No Candidozyma auris (form. Candida auris) CA5LBN GCA_019039755.1 No No Candidozyma auris (form. Candida auris) CA6LBN GCA_019039715.1 No No Candidozyma auris (form. Candida auris) CA7LBN GCA_019332045.1 No No Candidozyma auris (form. Candida auris) CA8LBN GCA_019039635.1 No No Candidozyma auris (form. Candida auris) CA9LBN GCA_019039695.1 No No Candidozyma auris (form. Candida auris) CA-AM1 GCA_014673535.1 No No Candidozyma auris (form. Candida auris) cama02 GCA_032694445.1 No No Candidozyma auris (form. Candida auris) cama03 GCA_032694655.1 No No Candidozyma auris (form. Candida auris) cama04 GCA_032694695.1 No No Candidozyma auris (form. Candida auris) cama05 GCA_032694685.1 No No Candidozyma auris (form. Candida auris) cama06 GCA_032694745.1 No No Candidozyma auris (form. Candida auris) cama07 GCA_032694645.1 No No Candidozyma auris (form. Candida auris) cama08 GCA_032694485.1 No No Candidozyma auris (form. Candida auris) cama09 GCA_032694465.1 No No Candidozyma auris (form. Candida auris) cama10 GCA_032694475.1 No No Candidozyma auris (form. Candida auris) Case 1 GCA_035582645.1 No No Candidozyma auris (form. Candida auris) Case 2 GCA_035582625.1 No No Candidozyma auris (form. Candida auris) Case 3 GCA_035582605.1 No No Candidozyma auris (form. Candida auris) Case 4 GCA_035582585.1 No No Candidozyma auris (form. Candida auris) Case 5 GCA_035582555.1 No No Candidozyma auris (form. Candida auris) Case 6 GCA_035582525.1 No No Candidozyma auris (form. Candida auris) Case 7 GCA_035582505.1 No No Candidozyma auris (form. Candida auris) CAU924 / 6920 GCA_019968925.1 No No Candidozyma auris (form. Candida auris) CBS 10913 GCA_030581515.1 No No Candidozyma auris (form. Candida auris) F0083 GCA_032367535.1 No No Candidozyma auris (form. Candida auris) F1580 GCA_032714025.1 No No Candidozyma auris (form. Candida auris) F3485 GCA_032715285.1 No No Candidozyma auris (form. Candida auris) FG_GE10 GCA_014638765.1 No No Candidozyma auris (form. Candida auris) FG_GE2 GCA_014638955.1 No No Candidozyma auris (form. Candida auris) FG_GE3 GCA_014638965.1 No No Candidozyma auris (form. Candida auris) FG_GE4 GCA_014638895.1 No No Candidozyma auris (form. Candida auris) FG_GE5 GCA_014638885.1 No No Candidozyma auris (form. Candida auris) FG_GE6 GCA_014638875.1 No No Candidozyma auris (form. Candida auris) FG_GE7 GCA_014638825.1 No No Candidozyma auris (form. Candida auris) FG_GE8 GCA_014638805.1 No No Candidozyma auris (form. Candida auris) FG_GE9 GCA_014638775.1 No No Candidozyma auris (form. Candida auris) GZ0008 GCA_035220645.1 No No Candidozyma auris (form. Candida auris) 1-172 GCA_027563765.1 No No Candidozyma auris (form. Candida auris) IFRC2087 GCA_016809505.1 No No Candidozyma auris (form. Candida auris) JCM 15448 GCA_007168705.1 No No Candidozyma auris (form. Candida auris) L1537 / 2020 GCA_020809265.1 No No Candidozyma auris (form. Candida auris) LMDM 1219 GCA_041381755.1 No No Candidozyma auris (form. Candida auris) LOM GCA_005234155.1 No No Candidozyma auris (form. Candida auris) MRSN101498 GCA_029619135.1 No No Candidozyma auris (form. Candida auris) MRSN455051 GCA_029618695.1 No No Candidozyma auris (form. Candida auris) MRSN455060 GCA_029618685.1 No No Candidozyma auris (form. Candida auris) MRSN455079 GCA_029619145.1 No No Candidozyma auris (form. Candida auris) MRSN456999 GCA_029618675.1 No No Candidozyma auris (form. Candida auris) PL1 GCA_026743825.1 No No Candidozyma auris (form. Candida auris) PUSH159 GCA_041381095.1 No No Candidozyma auris (form. Candida auris) RCPF-1821 GCA_004287075.1 No No Candidozyma auris (form. Candida auris) SCHI0087. S.148 GCA_044589395.1 No No Candidozyma auris (form. Candida auris) SCO_240 GCA_041026505.1 No No Candidozyma auris (form. Candida auris) SCO_242 GCA_041026585.1 No No Candidozyma auris (form. Candida auris) SCO_248 GCA_041026605.1 No No Candidozyma auris (form. Candida auris) SCO_266 GCA_041026525.1 No No Candidozyma auris (form. Candida auris) SCO_267 GCA_041026465.1 No No Candidozyma auris (form. Candida auris) SCO_275 GCA_041026565.1 No No Candidozyma auris (form. Candida auris) SCO_276 GCA_041026545.1 No No Candidozyma auris (form. Candida auris) SCO_279 GCA_041026485.1 No No Candidozyma auris (form. Candida auris) VPCI 479 / P / 13 GCA_001049995.1 No No Debaryomycetaceae sp. (form. P. dianae) ZIM 2471 GCA_031081905.1 No No Hyphopichia heimii (form. P. heimii) NRRL Y-7502 SRR6475888 No No Komagataella pastoris (form. P. pastoris) ATCC 28485 GCA_001708105.1 No No Komagataella phaffii (form. P. pastoris) GS115 ERR1294016 No No Komagataella phaffii (form. P. pastoris) X-33 SRR8244566 No No Komagataella phaffii (form. P. pastoris) Y-11430 SRR8244569 No No Komagataella phaffii (form. P. pastoris) Y-12729 SRR8244568 No No Komagataella phaffii (form. P. pastoris) Y-17741 SRR8244572 No No Komagataella phaffii (form. P. pastoris) Y-48123 SRR8244565 No No Komagataella phaffii (form. P. pastoris) Y-48124 SRR8244563 No No Komagataella phaffii (form. P. pastoris) Y-7556 SRR8244570 No No Komagataella phaffii (form. P. pastoris) YB-378 SRR8244571 No No Komagataella phaffii (form. P. pastoris) YB-4289 SRR8244564 No No Komagataella phaffii (form. P. pastoris) YB-4290 SRR8244567 No No Millerozyma farinosa (form. P. farinosa) CBS 7064 ERR331065 No No Cylindricascospora (form. Nakaseomyces) bacillisporus CBS

[0504] No No 7720 GCA_001046975.1

[0505] Cylindricascospora (form. Nakaseomyces) bacillisporus

[0506] No No NRRL Y-17846 GCA_030573655.1

[0507] Nakaseomyces bracarensis CBS 10154 GCA_001077315.1 No No Nakaseomyces bracarensis F7312 GCA_037041435.1 No No Nakaseomyces bracarensis NRRL Y-48270 GCA_030569875.1 No No Oligophagozyma (formerly Nakaseomyces) castellii CBS

[0508] No No 4332 GCA_001046935.1

[0509] Oligophagozyma (formerly Nakaseomyces) castellii NRRL Y- No No 17070 GCA_030556345.1

[0510] Nakaseomyces delphensis CBS 2170 GCA_001039675.1 No No Nakaseomyces delphensis NRRL Y-2379 GCA_030570895.1 No No Nakaseomyces glabratus 040 SRR8953803 No No Nakaseomyces glabratus 044 SRR8953802 No No Nakaseomyces glabratus 15.0126687 SRR14381471 No No Nakaseomyces glabratus 15-0122390 SRR14381442 No No Nakaseomyces glabratus 15-0126243 SRR14381467 No No Nakaseomyces glabratus 15-0126255 SRR14381463 No No Nakaseomyces glabratus 15-0126687 SRR14381471 No No Nakaseomyces glabratus 15-0452362 SRR14381468 No No Nakaseomyces glabratus 15-0455335 SRR14381462 No No Nakaseomyces glabratus 15-0456221 SRR14381461 No No Nakaseomyces glabratus 15-0456299 SRR14381460 No No Nakaseomyces glabratus 15-6104001 SRR14381457 No No Nakaseomyces glabratus 15-6110831 SRR14381456 No No Nakaseomyces glabratus 16.0107051 SRR14381445 No No Nakaseomyces glabratus 16.0452821 SRR14381444 No No Nakaseomyces glabratus 16.0453380 SRR14381446 No No Nakaseomyces glabratus 16.6715916 SRR14381447 No No Nakaseomyces glabratus 16.6793165 SRR14381458 No No Nakaseomyces glabratus 16-0107051 SRR14381445 No No Nakaseomyces glabratus 16-0117315 SRR14381459 No No Nakaseomyces glabratus 16-0127919 SRR14381455 No No Nakaseomyces glabratus 16-0128373 SRR14381454 No No Nakaseomyces glabratus 16-0128490 SRR14381453 No No Nakaseomyces glabratus 16-0451008 SRR14381465 No No Nakaseomyces glabratus 16-0452821 SRR14381444 No No Nakaseomyces glabratus 16-0453380 SRR14381446 No No Nakaseomyces glabratus 16-0453570 SRR14381452 No No Nakaseomyces glabratus 16-6527805 SRR14381466 No No Nakaseomyces glabratus 16-6616386 SRR14381450 No No Nakaseomyces glabratus 16-6715852 SRR14381469 No No Nakaseomyces glabratus 16-6715916 SRR14381447 No No Nakaseomyces glabratus 16-6793165 SRR14381458 No No Nakaseomyces glabratus 17.6506958 SRR14381451 No No Nakaseomyces glabratus 17.6715877 SRR14381470 No No Nakaseomyces glabratus 17.6789296 SRR14381443 No No Nakaseomyces glabratus 17-0102191 SRR14381449 No No Nakaseomyces glabratus 17-0450193 SRR14381448 No No Nakaseomyces glabratus 17-6506958 SRR14381451 No No Nakaseomyces glabratus 17-6715877 SRR14381470 No No Nakaseomyces glabratus 17-6789296 SRR14381443 No No Nakaseomyces glabratus 1A GCA_001466525.1 No No Nakaseomyces glabratus 1B GCA_001466535.1 No No Nakaseomyces glabratus 2018-F1-188-B ERR4669757 No No Nakaseomyces glabratus 2018-F5-18 ERR4669779 No No Nakaseomyces glabratus 2018-F6-160 ERR4669782 No No Nakaseomyces glabratus 2018-F6-162 ERR4669784 No No Nakaseomyces glabratus 2018-F6-164 ERR4669786 No No Nakaseomyces glabratus 2018-F6-165 ERR4669787 No No Nakaseomyces glabratus 2018-F6-167 ERR4669789 No No Nakaseomyces glabratus 2A GCA_001466635.1 No No Nakaseomyces glabratus 2B GCA_001466575.1 No No Nakaseomyces glabratus 3A GCA_001466685.1 No No Nakaseomyces glabratus 3B GCA_001466565.1 No No Nakaseomyces glabratus 50570 SRR14844980 No No Nakaseomyces glabratus 67367 SRR14844979 No No Nakaseomyces glabratus 73281 SRR14844978 No No Nakaseomyces glabratus AR-0314 SRR25168788 No No Nakaseomyces glabratus AR-0316 SRR25168783 No No Nakaseomyces glabratus AR-0318 SRR25168787 No No Nakaseomyces glabratus AR-0319 SRR25168785 No No Nakaseomyces glabratus AR-0320 SRR25168791 No No Nakaseomyces glabratus AR-0322 SRR25168784 No No Nakaseomyces glabratus AR-0323 SRR25168792 No No Nakaseomyces glabratus AR-0324 SRR25168789 No No Nakaseomyces glabratus AR-0325 SRR25168786 No No Nakaseomyces glabratus AR-0326 SRR25168790 No No Nakaseomyces glabratus AR-0327 SRR25177397 No No Nakaseomyces glabratus AR-0328 SRR25177394 No No Nakaseomyces glabratus AR-0329 SRR25177398 No No Nakaseomyces glabratus AR-0330 SRR25177402 No No Nakaseomyces glabratus AR-0581 SRR25169060 No No Nakaseomyces glabratus AR-0582 SRR25169059 No No Nakaseomyces glabratus AR-0583 SRR25169057 No No Nakaseomyces glabratus AR-0584 SRR25169056 No No Nakaseomyces glabratus AR-0585 SRR25169058 No No Nakaseomyces glabratus ATCC 2001 GCA_010111755.1 No No Nakaseomyces glabratus ATCC2001 CBS138 SRR10717941 No No Nakaseomyces glabratus B1514 GCA_010111755.1 No No Nakaseomyces glabratus BG2 SRR10877325 No No Nakaseomyces glabratus BG2 11B AinF SRR15498445 No No Nakaseomyces glabratus BG2 11B ANI SRR15498429 No No Nakaseomyces glabratus BG2 11B FinA SRR15498477 No No Nakaseomyces glabratus BG2 11B FLZ SRR15498478 No No Nakaseomyces glabratus BG2 11B YPD SRR15498489 No No Nakaseomyces glabratus BG2 11H AinF SRR15498492 No No Nakaseomyces glabratus BG2 11H ANI SRR15498443 No No Nakaseomyces glabratus BG2 11H FinA SRR15498475 No No Nakaseomyces glabratus BG2 11H FLZ SRR15498461 No No Nakaseomyces glabratus BG2 SRA WT SRR15498448 No No Nakaseomyces glabratus BG3993 GCA_020450195.1 No No Nakaseomyces glabratus BG3994 GCA_020450265.1 No No Nakaseomyces glabratus BG3995 GCA_020450215.1 No No Nakaseomyces glabratus BG3996 GCA_020450235.1 No No Nakaseomyces glabratus C10471 SRR23984272 No No Nakaseomyces glabratus C10472 SRR23984271 No No Nakaseomyces glabratus C10473 SRR23984270 No No Nakaseomyces glabratus C10474 SRR23984269 No No Nakaseomyces glabratus C10534 SRR23984268 No No Nakaseomyces glabratus C10535 SRR23984264 No No Nakaseomyces glabratus C10536 SRR23984267 No No Nakaseomyces glabratus C10537 SRR23984266 No No Nakaseomyces glabratus C10538 SRR23984265 No No Nakaseomyces glabratus C10539 SRR23984263 No No Nakaseomyces glabratus CAGL1256 SRR13441714 No No Nakaseomyces glabratus CAGL1875 SRR13441715 No No Nakaseomyces glabratus CAS08-0016 SRR14180811 No No Nakaseomyces glabratus CAS08-0027 SRR14180815 No No Nakaseomyces glabratus CAS08-0069 SRR8697397 No No Nakaseomyces glabratus CAS08-0123 SRR8697376 No No Nakaseomyces glabratus CAS08-0136 SRR8697452 No No Nakaseomyces glabratus CAS08-0154 SRR8697454 No No Nakaseomyces glabratus CAS08-0173 SRR8697407 No No Nakaseomyces glabratus CAS08-0190 SRR8697408 No No Nakaseomyces glabratus CAS08-0209 SRR8697364 No No Nakaseomyces glabratus CAS08-0222 SRR8697383 No No Nakaseomyces glabratus CAS08-0248 SRR8697402 No No Nakaseomyces glabratus CAS08-0313 SRR8697403 No No Nakaseomyces glabratus CAS08-0317 SRR8697474 No No Nakaseomyces glabratus CAS08-0343 SRR8697334 No No Nakaseomyces glabratus CAS08-0407 SRR8697439 No No Nakaseomyces glabratus CAS08-0423 SRR8697347 No No N

[0511]

[0512] akaseomyces glabratus CAS08-0425 SRR14180816 No No Nakaseomyces glabratus CAS08-0456 SRR8697379 No No Nakaseomyces glabratus CAS08-0458 SRR8697393 No No Nakaseomyces glabratus CAS08-0482 SRR8697333 No No Nakaseomyces glabratus CAS08-0525 SRR8697425 No No Nakaseomyces glabratus CAS08-0557 SRR8697366 No No Nakaseomyces glabratus CAS08-0559 SRR8697483 No No Nakaseomyces glabratus CAS08-0569 SRR8697493 No No Nakaseomyces glabratus CAS08-0616 SRR8697343 No No Nakaseomyces glabratus CAS08-0627 SRR8697427 No No Nakaseomyces glabratus CAS08-0647 SRR8697428 No No Nakaseomyces glabratus CAS08-0725 SRR8697475 No No Nakaseomyces glabratus CAS09-0751 SRR8697308 No No Nakaseomyces glabratus CAS09-0811 SRR8697466 No No Nakaseomyces glabratus CAS09-0818 SRR8697461 No No Nakaseomyces glabratus CAS09-0872 SRR8697309 No No Nakaseomyces glabratus CAS09-0901 SRR8697302 No No Nakaseomyces glabratus CAS09-0930 SRR8697412 No No Nakaseomyces glabratus CAS09-0993 SRR8697424 No No Nakaseomyces glabratus CAS09-0997 SRR8697372 No No Nakaseomyces glabratus CAS09-1021 SRR8697327 No No Nakaseomyces glabratus CAS09-1091 SRR8697328 No No Nakaseomyces glabratus CAS09-1195 SRR8697367 No No Nakaseomyces glabratus CAS09-1352 SRR8697492 No No Nakaseomyces glabratus CAS09-1450 SRR8697441 No No Nakaseomyces glabratus CAS09-1513 SRR8697489 No No Nakaseomyces glabratus CAS09-1601 SRR8697387 No No Nakaseomyces glabratus CAS09-1622 SRR8697371 No No Nakaseomyces glabratus CAS09-1798 SRR8697346 No No Nakaseomyces glabratus CAS09-1809 SRR8697354 No No Nakaseomyces glabratus CAS10- 1832 SRR8697494 No No Nakaseomyces glabratus CAS10- 1862 SRR8697396 No No Nakaseomyces glabratus CAS10- 1893 SRR8697384 No No Nakaseomyces glabratus CAS10-1895 SRR8697480 No No Nakaseomyces glabratus CAS10- 1919 SRR8697490 No No Nakaseomyces glabratus CAS10- 1922 SRR8697414 No No Nakaseomyces glabratus CAS10- 1929 SRR8697270 No No Nakaseomyces glabratus CAS10- 1964 SRR8697267 No No Nakaseomyces glabratus CAS10-1984 SRR8697458 No No Nakaseomyces glabratus CAS10- 1986 SRR8697394 No No Nakaseomyces glabratus CAS10- 2006 SRR8697395 No No Nakaseomyces glabratus CAS10-2124 SRR8697271 No No Nakaseomyces glabratus CAS10-2289 SRR8697352 No No Nakaseomyces glabratus CAS10-2308 SRR8697365 No No Nakaseomyces glabratus CAS10- 2449 SRR8697479 No No Nakaseomyces glabratus CAS10- 2472 SRR8697434 No No Nakaseomyces glabratus CAS10- 2476 SRR8697449 No No Nakaseomyces glabratus CAS10- 2478 SRR8697382 No No Nakaseomyces glabratus CAS10-2533 SRR8697377 No No Nakaseomyces glabratus CAS10-2547 SRR8697433 No No Nakaseomyces glabratus CAS10-2558 SRR8697411 No No Nakaseomyces glabratus CAS10-2559 SRR8697443 No No Nakaseomyces glabratus CAS10-2592 SRR8697363 No No Nakaseomyces glabratus CAS10-2626 SRR8697410 No No Nakaseomyces glabratus CAS10- 2717 SRR8697272 No No Nakaseomyces glabratus CAS10- 2720 SRR8697321 No No Nakaseomyces glabratus CAS10-2732 SRR8697349 No No Nakaseomyces glabratus CAS11- 2802 SRR8697436 No No Nakaseomyces glabratus CAS11- 2817 SRR8697465 No No Nakaseomyces glabratus CAS11- 2831 SRR8697447 No No Nakaseomyces glabratus CAS11- 2834 SRR8697457 No No Nakaseomyces glabratus CAS11-2853 SRR8697276 No No Nakaseomyces glabratus CAS11- 2870 SRR8697374 No No Nakaseomyces glabratus CAS11- 2931 SRR8697368 No No Nakaseomyces glabratus CAS11- 2934 SRR8697341 No No Nakaseomyces glabratus CAS11- 2972 SRR8697342 No No Nakaseomyces glabratus CAS11-2978 SRR8697340 No No Nakaseomyces glabratus CAS11- 2985 SRR8697472 No No Nakaseomyces glabratus CAS11-3018 SRR8697462 No No Nakaseomyces glabratus CAS11-3122 SRR8697467 No No Nakaseomyces glabratus CAS11-3129 SRR8697269 No No Nakaseomyces glabratus CAS11-3232 SRR8697344 No No Nakaseomyces glabratus CAS11-3233 SRR8697456 No No Nakaseomyces glabratus CAS11-3234 SRR8697463 No No Nakaseomyces glabratus CAS11-3247 SRR8697437 No No Nakaseomyces glabratus CAS11-3264 SRR8697331 No No Nakaseomyces glabratus CAS11-3345 SRR8697320 No No Nakaseomyces glabratus CAS11-3395 SRR8697476 No No Nakaseomyces glabratus CAS11-3433 SRR8697401 No No Nakaseomyces glabratus CAS11-3481 SRR8697369 No No Nakaseomyces glabratus CAS11-3482 SRR8697375 No No Nakaseomyces glabratus CAS12-3674 SRR8697350 No No Nakaseomyces glabratus CAS12-3684 SRR8697392 No No Nakaseomyces glabratus CAS12-3690 SRR8697405 No No Nakaseomyces glabratus CAS12-3692 SRR8697429 No No Nakaseomyces glabratus CAS12-3708 SRR8697477 No No Nakaseomyces glabratus CAS12-3737 SRR8697300 No No Nakaseomyces glabratus CAS12-3739 SRR8697419 No No Nakaseomyces glabratus CAS12-3900 SRR8697481 No No Nakaseomyces glabratus CAS12-3946 SRR8697358 No No Nakaseomyces glabratus CAS12-3951 SRR8697353 No No Nakaseomyces glabratus CAS12-3980 SRR8697448 No No Nakaseomyces glabratus CAS12-3983 SRR8697404 No No Nakaseomyces glabratus CAS12-3998 SRR8697362 No No Nakaseomyces glabratus CAS12-4046 SRR8697471 No No Nakaseomyces glabratus CAS12-4099 SRR8697389 No No Nakaseomyces glabratus CAS12-4162 SRR8697399 No No Nakaseomyces glabratus CAS12-4163 SRR8697453 No No Nakaseomyces glabratus CAS12-4176 SRR8697264 No No Nakaseomyces glabratus CAS12-4212 SRR8697275 No No Nakaseomyces glabratus CAS12-4215 SRR8697426 No No Nakaseomyces glabratus CAS12-4242 SRR8697361 No No Nakaseomyces glabratus CAS12-4323 SRR8697451 No No Nakaseomyces glabratus CAS12-4380 SRR8697317 No No Nakaseomyces glabratus CAS12-4440 SRR8697303 No No Nakaseomyces glabratus CAS13-4493 SRR8697325 No No Nakaseomyces glabratus CAS13-4530 SRR8697440 No No Nakaseomyces glabratus CAS13-4641 SRR8697417 No No Nakaseomyces glabratus CAS13-4648 SRR8697335 No No Nakaseomyces glabratus CAS13-4735 SRR8697388 No No Nakaseomyces glabratus CAS13-4776 SRR8697373 No No Nakaseomyces glabratus CAS13-4777 SRR8697322 No No Nakaseomyces glabratus CAS13-4778 SRR8697273 No No Nakaseomyces glabratus CAS13-4791 SRR8697360 No No Nakaseomyces glabratus CAS13-4813 SRR8697323 No No Nakaseomyces glabratus CAS13-4841 SRR8697386 No No Nakaseomyces glabratus CAS13-4862 SRR8697378 No No Nakaseomyces glabratus CAS13-4878 SRR8697398 No No Nakaseomyces glabratus CAS13-4970 SRR8697416 No No Nakaseomyces glabratus CAS13-5030 SRR8697406 No No Nakaseomyces glabratus CAS13-5037 SRR8697277 No No Nakaseomyces glabratus CAS13-5109 SRR8697345 No No Nakaseomyces glabratus CAS13-5134 SRR8697307 No No Nakaseomyces glabratus CAS13-5140 SRR8697464 No No Nakaseomyces glabratus CAS13-5158 SRR8697488 No No Nakaseomyces glabratus CAS13-5210 SRR8697444 No No Nakaseomyces glabratus CAS13-5237 SRR8697418 No No Nakaseomyces glabratus CAS13-5239 SRR8697324 No No Nakaseomyces glabratus CAS13-5245 SRR8697445 No No Nakaseomyces glabratus CAS14-5293 SRR8697478 No No Nakaseomyces glabratus CAS14-5468 SRR8697381 No No Nakaseomyces glabratus CAS14-5492 SRR8697430 No No Nakaseomyces glabratus CAS14-5518 SRR8697413 No No Nakaseomyces glabratus CAS14-5563 SRR8697482 No No Nakaseomyces glabratus CAS14-5589 SRR8697319 No No Nakaseomyces glabratus CAS14-5591 SRR8697468 No No Nakaseomyces glabratus CAS14-5647 SRR8697491 No No Nakaseomyces glabratus CAS14-5770 SRR8697356 No No Nakaseomyces glabratus CAS14-5851 SRR8697329 No No Nakaseomyces glabratus CAS14-5908 SRR8697355 No No Nakaseomyces glabratus CAS14-5982 SRR8697351 No No Nakaseomyces glabratus CAS14-5984 SRR8697422 No No Nakaseomyces glabratus CAS14-5989 SRR8697370 No No Nakaseomyces glabratus CAS14-6054 SRR8697326 No No Nakaseomyces glabratus CAS15-6171 SRR8697469 No No Nakaseomyces glabratus CAS15-6184 SRR8697495 No No Nakaseomyces glabratus CAS15-6272 SRR8697301 No No Nakaseomyces glabratus CAS15-6297 SRR8697460 No No Nakaseomyces glabratus CAS15-6327 SRR8697304 No No Nakaseomyces glabratus CAS15-6394 SRR8697390 No No Nakaseomyces glabratus CAS15-6404 SRR8697409 No No Nakaseomyces glabratus CAS15-6472 SRR8697450 No No Nakaseomyces glabratus CAS15-6569 SRR8697336 No No Nakaseomyces glabratus CAS15-6616 SRR86973O6 No No Nakaseomyces glabratus CAS15-6673 SRR869733O No No Nakaseomyces glabratus CAS15-6682 SRR8697438 No No Nakaseomyces glabratus CAS15-6812 SRR8697455 No No Nakaseomyces glabratus CAS15-6814 SRR8697446 No No Nakaseomyces glabratus CAS16-6915 SRR8697332 No No Nakaseomyces glabratus CAS16-6927 SRR8697338 No No Nakaseomyces glabratus CAS16-6951 SRR8697357 No No Nakaseomyces glabratus CAS16-7048 SRR8697359 No No Nakaseomyces glabratus CAS16-7105 SRR8697421 No No Nakaseomyces glabratus CAS16-7155 SRR8697299 No No Nakaseomyces glabratus CAS16-7332 SRR8697470 No No Nakaseomyces glabratus CAS16-7378 SRR8697268 No No Nakaseomyces glabratus CAS16-7380 SRR8697431 No No Nakaseomyces glabratus CAS16-7403 SRR8697266 No No Nakaseomyces glabratus CAS16-7419 SRR8697385 No No Nakaseomyces glabratus CAS16-7440 SRR8697339 No No Nakaseomyces glabratus CAS16-7442 SRR8697337 No No Nakaseomyces glabratus CAS16-7574 SRR8697442 No No Nakaseomyces glabratus CAS16-7575 SRR8697432 No No Nakaseomyces glabratus CAS16-7596 SRR8697274 No No Nakaseomyces glabratus CAS17-7689 SRR8697400 No No Nakaseomyces glabratus CAS17-7704 SRR8697423 No No Nakaseomyces glabratus CAS17-7712 SRR8697348 No No Nakaseomyces glabratus CAS17-7728 SRR8697435 No No Nakaseomyces glabratus CAS17-7768 SRR8697278 No No Nakaseomyces glabratus CBS138 GCA_000002545.2 No No Nakaseomyces glabratus CBS1389F AinF SRR15498495 No No Nakaseomyces glabratus CBS1389F ANI SRR15498425 No No Nakaseomyces glabratus CBS1389F FinA SRR15498474 No No Nakaseomyces glabratus CBS1389F FLZ SRR15498497 No No Nakaseomyces glabratus CBS1389F YPD SRR15498479 No No Nakaseomyces glabratus CBS1389H AinF SRR15498485 No No Nakaseomyces glabratus CBS1389H ANI SRR15498449 No No Nakaseomyces glabratus CBS1389H FinA SRR15498483 No No Nakaseomyces glabratus CBS1389H FLZ SRR15498471 No No Nakaseomyces glabratus CBS138 SRA WT SRR15498470 No No Nakaseomyces glabratus CCTCC M202019 GCA_000497105.2 No No Nakaseomyces glabratus CG107a SRR1282523O No No Nakaseomyces glabratus CG107b SRR12825229 No No Nakaseomyces glabratus CG121 SRR1282525O No Nakaseomyces glabratus CG124 SRR12825249 No Nakaseomyces glabratus CG127 SRR12825248 No Nakaseomyces glabratus CG133 SRR12825247 No Nakaseomyces glabratus CG134 SRR12825246 No Nakaseomyces glabratus CG144 SRR12825245 No Nakaseomyces glabratus CG146 SRR12825244 No Nakaseomyces glabratus CG150 SRR12825242 No Nakaseomyces glabratus CG151 SRR12825241 No Nakaseomyces glabratus CG153 SRR1282524O No Nakaseomyces glabratus CG157 SRR12825239 No Nakaseomyces glabratus CG162 SRR12825238 No Nakaseomyces glabratus CG166 SRR12825237 No Nakaseomyces glabratus CG167 SRR12825236 No Nakaseomyces glabratus CG174 SRR12825235 No Nakaseomyces glabratus CG181 SRR12825234 No Nakaseomyces glabratus CG18a SRR12825228 No Nakaseomyces glabratus CG18b SRR12825227 No Nakaseomyces glabratus CG191a SRR12825226 No Nakaseomyces glabratus CG191b SRR12825225 No Nakaseomyces glabratus CG191c SRR12825224 No Nakaseomyces glabratus CG191d SRR12825223 No Nakaseomyces glabratus CG191e SRR12825222 No Nakaseomyces glabratus CG191f SRR1282522O No Nakaseomyces glabratus CG203 SRR12825231 No Nakaseomyces glabratus CG42 SRR12825243 No Nakaseomyces glabratus CG43 SRR12825232 No Nakaseomyces glabratus CG46 SRR12825221 No Nakaseomyces glabratus CG48a SRR12825219 No Nakaseomyces glabratus CG48f SRR12825217 No Nakaseomyces glabratus CG48h SRR12825216 No Nakaseomyces glabratus CG52 SRR1282521O No Nakaseomyces glabratus CG53 SRR12825199 No Nakaseomyces glabratus CG54 SRR12825196 No Nakaseomyces glabratus CG55 SRR12825195 No Nakaseomyces glabratus CG68a SRR12825215 No Nakaseomyces glabratus CG69b SRR12825214 No Nakaseomyces glabratus CG84f SRR12825213 No Nakaseomyces glabratus CG84g SRR12825212 No Nakaseomyces glabratus CG93a SRR128252O9 No Nakaseomyces glabratus CG93b SRR128252O8 No Nakaseomyces glabratus CG93c SRR128252O7 No Nakaseomyces glabratus CG93d SRR128252O6 No Nakaseomyces glabratus CG93e SRR128252O5 No Nakaseomyces glabratus CG93h SRR128252O4 No Nakaseomyces glabratus CG93i SRR128252O3 No Nakaseomyces glabratus CG93j SRR128252O2 No Nakaseomyces glabratus CG93k SRR128252O1 No Nakaseomyces glabratus CG97a SRR12825200 No No Nakaseomyces glabratus CG97b SRR12825198 No No Nakaseomyces glabratus CG97c SRR12825197 No No Nakaseomyces glabratus CLIB 3042 ERR9706985 No No Nakaseomyces glabratus CST342A AinF SRR15498503 No No Nakaseomyces glabratus CST342A ANI SRR15498440 No No Nakaseomyces glabratus CST342A ANIFLZ SRR15498473 No No Nakaseomyces glabratus CST342A FinA SRR15498430 No No Nakaseomyces glabratus CST342A FLZ SRR15498458 No No Nakaseomyces glabratus CST342A YPD SRR15498463 No No Nakaseomyces glabratus CST342C FinA SRR15498462 No No Nakaseomyces glabratus CST342C FLZ SRR15498499 No No Nakaseomyces glabratus CST342G AinF SRR15498457 No No Nakaseomyces glabratus CST342G ANI SRR15498451 No No Nakaseomyces glabratus CST342G ANIFLZ SRR15498467 No No Nakaseomyces glabratus CST784G FinA SRR15498434 No No Nakaseomyces glabratus CST784G FLZ SRR15498496 No No Nakaseomyces glabratus CST784G YPD SRR15498493 No No Nakaseomyces glabratus DPK305 SRR14180810 No No Nakaseomyces glabratus DPK762 SRR14180814 No No Nakaseomyces glabratus DPL1021 SRR14180813 No No Nakaseomyces glabratus DPL245 SRR14180812 No No Nakaseomyces glabratus DSY562 GCA_002219185.1 No No Nakaseomyces glabratus DSY565 GCA_002219195.1 No No Nakaseomyces glabratus EB09113B AinF SRR15498488 No No Nakaseomyces glabratus EB09113B ANI SRR15498427 No No Nakaseomyces glabratus EB09113B FinA SRR15498452 No No Nakaseomyces glabratus EB09113B FLZ SRR15498436 No No Nakaseomyces glabratus EB09113B YPD SRR15498472 No No Nakaseomyces glabratus EB09113H AinF SRR15498494 No No Nakaseomyces glabratus EB09113H ANI SRR15498464 No No Nakaseomyces glabratus EB09113H FinA SRR15498444 No No Nakaseomyces glabratus EB09113H FLZ SRR15498484 No No Nakaseomyces glabratus EB0911Sto SRR15498487 No No Nakaseomyces glabratus EF16207B AinF SRR15498460 No No Nakaseomyces glabratus EF16207B ANI SRR15498456 No No Nakaseomyces glabratus EF16207B FinA SRR15498480 No No Nakaseomyces glabratus EF16207B FLZ SRR15498439 No No Nakaseomyces glabratus EF16207D AinF SRR15498438 No No Nakaseomyces glabratus EF16207D ANI SRR15498482 No No Nakaseomyces glabratus EF16207D ANIFLZ SRR15498466 No No Nakaseomyces glabratus EF16207D FinA SRR15498500 No No Nakaseomyces glabratus EF16207D FLZ SRR15498476 No No Nakaseomyces glabratus EF16207D YPD SRR15498465 No No Nakaseomyces glabratus EF16207F AinF SRR15498432 No No Nakaseomyces glabratus EF16207F ANI SRR15498431 No No Nakaseomyces glabratus EF16207F ANIFLZ SRR1549849O No No Nakaseomyces glabratus ERZ1036435.336 GCA_937870575.1 No No Nakaseomyces glabratus F158G FinA SRR15498455 No No Nakaseomyces glabratus F158G FLZ SRR15498447 No No Nakaseomyces glabratus F158G YPD SRR15498423 No No Nakaseomyces glabratus F15 SRA WT SRR15498486 No No Nakaseomyces glabratus FFUL887 GCA_900166875.1 No No Nakaseomyces glabratus G_18_l SRR19696157 No No Nakaseomyces glabratus G_18_10 SRR19696156 No No Nakaseomyces glabratus G_18_11 SRR19696155 No No Nakaseomyces glabratus G_18_12 SRR19696154 No No Nakaseomyces glabratus G_18_13 SRR19696153 No No Nakaseomyces glabratus G_18_14 SRR19696152 No No Nakaseomyces glabratus G_18_15 SRR19696151 No No Nakaseomyces glabratus G_18_16 SRR1969615O No No Nakaseomyces glabratus G_18_2 SRR19696149 No No Nakaseomyces glabratus G_18_3 SRR19696147 No No Nakaseomyces glabratus G_18_4 SRR19696146 No No Nakaseomyces glabratus G_18_5 SRR19696145 No No Nakaseomyces glabratus G_18_6 SRR19696144 No No Nakaseomyces glabratus G_18_7 SRR19696143 No No Nakaseomyces glabratus G_18_8 SRR19696142 No No Nakaseomyces glabratus G_18_9 SRR19696141 No No Nakaseomyces glabratus G_19_1 SRR19696140 No No Nakaseomyces glabratus G_19_10 SRR19696139 No No Nakaseomyces glabratus G_19_11 SRR19696138 No No Nakaseomyces glabratus G_19_12 SRR19696136 No No Nakaseomyces glabratus G_19_13 SRR19696135 No No Nakaseomyces glabratus G_19_14 SRR19696134 No No Nakaseomyces glabratus G_19_15 SRR19696133 No No Nakaseomyces glabratus G_19_16 SRR19696132 No No Nakaseomyces glabratus G_19_17 SRR19696131 No No Nakaseomyces glabratus G_19_18 SRR1969613O No No Nakaseomyces glabratus G_19_19 SRR19696O93 No No Nakaseomyces glabratus G_19_2 SRR19696O92 No No Nakaseomyces glabratus G_19_20 SRR19696O91 No No Nakaseomyces glabratus G_19_21 SRR19696O89 No No Nakaseomyces glabratus G_19_3 SRR19696O88 No No Nakaseomyces glabratus G_19_4 SRR19696087 No No Nakaseomyces glabratus G_19_5 SRR19696O86 No No Nakaseomyces glabratus G_19_6 SRR19696O85 No No Nakaseomyces glabratus G_19_7 SRR19696084 No No Nakaseomyces glabratus G_19_8 SRR19696O83 No No Nakaseomyces glabratus G_19_9 SRR19696O82 No No Nakaseomyces glabratus G_20_l SRR19696O81 No No Nakaseomyces glabratus G_20_10 SRR19696O8O No No Nakaseomyces glabratus G_20_11 SRR19696078 No No Nakaseomyces glabratus G_20_12 SRR19696077 No No Nakaseomyces glabratus G_20_13 SRR19696O76 No No Nakaseomyces glabratus G_20_14 SRR19696O75 No No Nakaseomyces glabratus G_20_2 SRR19696074 No No Nakaseomyces glabratus G_20_3 SRR19696073 No No Nakaseomyces glabratus G_20_4 SRR19696072 No No Nakaseomyces glabratus G_20_6 SRR19696071 No No Nakaseomyces glabratus G_20_7 SRR19696070 No No Nakaseomyces glabratus G_20_8 SRR19696069 No No Nakaseomyces glabratus G_20_9 SRR19696265 No No Nakaseomyces glabratus G_21_1A SRR19696264 No No Nakaseomyces glabratus G_21_2 SRR19696263 No No Nakaseomyces glabratus G_21_3 SRR19696262 No No Nakaseomyces glabratus G_21_5A SRR19696260 No No Nakaseomyces glabratus IHEM 26304 SRR10725597 No No Nakaseomyces glabratus IHEM 9556 SRR10725599 No No Nakaseomyces glabratus ISTA29 SRR13648885 No No Nakaseomyces glabratus ISTB218 SRR13648884 No No Nakaseomyces glabratus M12 5F AinF SRR15498501 No No Nakaseomyces glabratus M12 5F ANI SRR15498481 No No Nakaseomyces glabratus M12 5F FinA SRR15498437 No No Nakaseomyces glabratus M12 5F YPD SRR15498450 No No Nakaseomyces glabratus M12 5H AinF SRR15498426 No No Nakaseomyces glabratus M12 5H ANI SRR15498453 No No Nakaseomyces glabratus M12 5H FinA SRR15498433 No No Nakaseomyces glabratus M12 5H FLZ SRR15498446 No No Nakaseomyces glabratus M1215 GCA_023629135.1 No No Nakaseomyces glabratus M6 GCA_025766415.1 No No Nakaseomyces glabratus ML72254 SRR17163935 No No Nakaseomyces glabratus MY07 GCA_022702475.2 No No Nakaseomyces glabratus NRRLY-65 SRR16974273 No No Nakaseomyces glabratus OL152 GCA_004332465.1 No No Nakaseomyces glabratus P35 10E AinF SRR15498498 No No Nakaseomyces glabratus P35 10E ANI SRR15498454 No No Nakaseomyces glabratus P35 10E ANIFLZ SRR15498441 No No Nakaseomyces glabratus P35 10E FinA SRR15498468 No No Nakaseomyces glabratus P35 10E FLZ SRR15498435 No No Nakaseomyces glabratus P35 10E YPD SRR15498469 No No Nakaseomyces glabratus P35 10G AinF SRR15498428 No No Nakaseomyces glabratus P35 10G ANI SRR15498459 No No Nakaseomyces glabratus P35 10G ANIFLZ SRR15498424 No No Nakaseomyces glabratus P35 10G FinA SRR15498491 No No Nakaseomyces glabratus P35 10G FLZ SRR15498442 No No Nakaseomyces glabratus P35-2 GCA_025331905.1 No No Nakaseomyces glabratus PK3901 GCA_025503555.1 No No Nakaseomyces glabratus PUJ-HUSI 916 SRR13441713 No No Nakaseomyces glabratus SCHI0137. M.1 GCA_044592685.1 No No Nakaseomyces glabratus SP1643 SRR11235429 No No Nakaseomyces glabratus SP2320 SRR11235428 No No Nakaseomyces glabratus SP2659 SRR11235427 No No Nakaseomyces glabratus SP2982 SRR11235422 No No Nakaseomyces glabratus SP3003 SRR11235421 No No Nakaseomyces glabratus SP3046 SRR11235424 No No Nakaseomyces glabratus SP3417 SRR11235426 No No Nakaseomyces glabratus SP3439 SRR11235423 No No Nakaseomyces glabratus SP3689 SRR11235425 No No Nakaseomyces glabratus UAB047-W10D4 GCA_002259835.1 No No Nakaseomyces glabratus UHNDB10192 GCA_021498375.1 No No Nakaseomyces glabratus UHNDB8982 GCA_021498365.1 No No Nakaseomyces glabratus UHNDB9150 GCA_021498435.1 No No Nakaseomyces glabratus UHNDB9664 GCA_021498385.1 No No Nakaseomyces glabratus Unknown SRR24010543 No No Nakaseomyces kungkrabaensis CBS 10927 GCA_030556385.1 No No Nakaseomyces nivariensis CBS 9983 GCA_001046915.1 No No Nakaseomyces nivariensis J HU Cniv vl GCA_017309295.1 No No Nakaseomyces nivariensis NRRL Y-48269 GCA_030569795.1 No No Nakaseomyces sp ABEgB8 SRR7007755 No No Nakaseomyces sp nov ABEgT12 SRR7007756 No No Nakaseomyces sp UFMG-CM-Y6046 SRR16974450 No No Nakaseomyces uthaithaninus 180604 SRR16974279 No No Nakaseomyces uthaithaninus CBS 10932 GCA_030564085.1 No No Ogataea kodamae (form. P. kodamae) NRRL Y-17234 SRR6475947 No No Ogataea polymorpha (form. P. angusta) NCYC 495 leul.l SRR3926512 No No Ogataea trehaloabstinens (form. P. trehaloabstinens) NRRL

[0513] No No Y-27595 SRR6475949

[0514] Phaffomyces antillensis (form. P. antillensis) NRRL Y-12881 SRR6475928 No No Phaffomyces opuntiae (form. P. opuntiae) NRRL Y-11707 SRR6475931 No No Phaffomyces thermotolerans (form. P. opuntiae var.

[0515] No No thermotolerans) NRRLY-11709 SRR6475929

[0516] Pichia cabralensis (form. C. cabralensis) CBS 11679 GCA_030569495.1 No No Pichia cactophila CBS 6926 GCA_039700745.1 No No Pichia cactophila NRRL Y-10963 GCA_030563965.1 No No Pichia californica (form. C. californica) 05-03-06(2) SRR4451748 No No Pichia californica (form. C. californica) NRRLY-27254 GCA_030574395.1 No No Pichia californica (form. C. californica) Olga-1 GCA_016584265.1 No No Pichia californica (form. C. californica) Olga-2 GCA_016584195.1 No No Pichia californica (form. C. californica) SB-116 SRR12998347 No No Pichia californica (form. C. californica) SB-48 SRR12998349 No No Pichia cephalocereana NRRL Y-17225 GCA_030565065.1 No No Pichia deserticola NRRLY-12918 GCA_030570135.1 No No Pichia eremophila NRRL Y-17224 GCA_030579415.1 No No Pichia ethanolica (form. C. ethanolica) M2 GCA_001649435.1 No No Pichia ethanolica (form. C. ethanolica) NRRLY-12615 GCA_030570435.1 No No Pichia exigua NRRL Y-10920 GCA_030570195.1 No No Pichia fermentans CDLB. YE03 GCA_035610515.1 No No Pichia fermentans CUB 258 ERR9707013 No No Pichia fermentans CUB 742 ERR9707004 No No Pichia fermentans F124-03 GCA_037041205.1 No No Pichia fermentans F5206 GCA_037041255.1 No No Pichia fermentans fo / MP / 02 GCA_003339355.1 No No Pichia fermentans KUST-G0103 GCA_030770065.1 No No Pichia fermentans NCYC 562 GCA_029581925.1 No No Pichia fermentans NRRLY-1619 GCA_030570255.1 No No Pichia fermentans PG 124-03 GCA_037041515.1 No No Pichia fermentans R5424 GCA_037041755.1 No No Pichia gijzeniarum CBS 15024 GCA_030556995.1 No No Pichia heedii NRRL Y-10967 GCA_030579495.1 No No Pichia inconspicua (form. C. inconspicua) 1282 SRR8506595 No No Pichia inconspicua (form. C. inconspicua) 14ANR23920 SRR8506591 No No Pichia inconspicua (form. C. inconspicua) 9_16 SRR8506590 No No Pichia inconspicua (form. C. inconspicua) CBS 180 GCA_004931855.1 No No Pichia inconspicua (form. C. inconspicua) Cll SRR8506589 No No Pichia inconspicua (form. C. inconspicua) CNM_CL6867 SRR8506594 No No Pichia inconspicua (form. C. inconspicua) IUM_96-0030 SRR8506593 No No Pichia inconspicua (form. C. inconspicua) LL867 SRR8506588 No No Pichia inconspicua (form. C. inconspicua) M1218 GCA_025531555.1 No No Pichia inconspicua (form. C. inconspicua) M126-08 GCA_037040195.1 No No Pichia inconspicua (form. C. inconspicua) NRRL Y-2029 GCA_030569135.1 No No Pichia inconspicua (form. C. inconspicua) R1214 GCA_025531545.1 No No Pichia inconspicua (form. C. inconspicua) UCSC_1590 SRR8506597 No No Pichia insulana CBS 11169 GCA_030558495.1 No No Pichia kluyveri 246 GCA_947297755.1 No No Pichia kluyveri APC 11.10 B GCA_030062975.1 No No Pichia kluyveri B7036 GCA_023629335.1 No No Pichia kluyveri CBA6002 GCA_003327685.1 No No Pichia kluyveri CUB 1225 ERR9707000 No No Pichia kluyveri DX115-08 GCA_037040945.1 No No Pichia kluyveri NCYC 246 ERR10466323 No No Pichia kluyveri NRRL Y-11519 GCA_030564505.1 No No Pichia kluyveri PK-24 GCA_037102705.1 No No Pichia kluyveri UCDFST 05-608 GCA_029581865.1 No No Pichia kluyveri X31-10 GCA_019096115.1 No No Pichia kudriavzevii 1163J SRR18739957 No No Pichia kudriavzevii 1173 SRR18739949 No No Pichia kudriavzevii 1286 SRR18739955 No No Pichia kudriavzevii 129 GCF_001983325.1 No No Pichia kudriavzevii 1372J SRR18739958 No No Pichia kudriavzevii 1715 SRR18739953 No No Pichia kudriavzevii 19 SRR18739951 No No Pichia kudriavzevii 2018-F1-211 ERR4669761 No No Pichia kudriavzevii 2018-F1-342 ERR4669763 No No Pichia kudriavzevii 2018-F1-51 ERR4669796 No No Pichia kudriavzevii 2018-F1-52 ERR4669748 No No Pichia kudriavzevii 2018-F6-163 ERR4669785 No No Pichia kudriavzevii 2018-F6-166 ERR4669788 No No Pichia kudriavzevii 2018-F7-71 ERR4669792 No No Pichia kudriavzevii 8110516017 ERR7438463 No No Pichia kudriavzevii AC-490 SRR24010555 No No Pichia kudriavzevii ASCUSDY21 GCA_019843505.1 No No Pichia kudriavzevii ATCC SRR18739964 No No Pichia kudriavzevii B036 SRR18739959 No No Pichia kudriavzevii B114-03 GCA_037040875.1 No No Pichia kudriavzevii B143 GCA_018105815.1 No No Pichia kudriavzevii B67 SRR18739961 No No Pichia kudriavzevii B7027 GCA_023629575.1 No No Pichia kudriavzevii C-AR1 SRR6977192 No No Pichia kudriavzevii CBS5147 GCA_003054405.1 No No Pichia kudriavzevii CBS573 GCA_003054445.1 No No Pichia kudriavzevii C-IT1 SRR6977184 No No Pichia kudriavzevii Ckrusei653 GCA_002166775.1 No No Pichia kudriavzevii CLIB1212 ERR9706989 No No Pichia kudriavzevii CLIB3242 ERR9706997 No No Pichia kudriavzevii C-LK1 SRR6977179 No No Pichia kudriavzevii CTeuk-1743 GCA_029290715.1 No No Pichia kudriavzevii CY902 GCA_014873065.1 No No Pichia kudriavzevii D108-B21 GCA_023629435.1 No No Pichia kudriavzevii E-GH1 SRR6977168 No No Pichia kudriavzevii E-JP2 SRR6977173 No No Pichia kudriavzevii E-JP3 SRR6977172 No No Pichia kudriavzevii E-UK1 SRR6977174 No No Pichia kudriavzevii E-WIl SRR6977177 No No Pichia kudriavzevii F2008 GCA_023629555.1 No No Pichia kudriavzevii F2020 GCA_023629635.1 No No Pichia kudriavzevii F4008B GCA_023629535.1 No No Pichia kudriavzevii HJ2 GCA_030264835.1 No No Pichia kudriavzevii 10001 SRR10402504 No No Pichia kudriavzevii 10002 SRR10402503 No No Pichia kudriavzevii 10003 SRR10402505 No No Pichia kudriavzevii 10004 SRR10403089 No No Pichia kudriavzevii 10005 SRR10403306 No No Pichia kudriavzevii 10006 SRR10405243 No No Pichia kudriavzevii 10007 SRR10405280 No No Pichia kudriavzevii 10008 SRR10405281 No No Pichia kudriavzevii 10009 SRR10405248 No No Pichia kudriavzevii 10010 SRR10405284 No No Pichia kudriavzevii 10011 SRR10405288 No No Pichia kudriavzevii 10012 SRR10405289 No No Pichia kudriavzevii 10013 SRR10406141 No No Pichia kudriavzevii 10015 SRR10406146 No No Pichia kudriavzevii 10016 SRR10406152 No No Pichia kudriavzevii 10017 SRR10406155 No No Pichia kudriavzevii 10018 SRR10406144 No No Pichia kudriavzevii 10019 SRR10406150 No No Pichia kudriavzevii 10020 SRR10406145 No No Pichia kudriavzevii 10021 SRR10406154 No No Pichia kudriavzevii 10022 SRR10406153 No No Pichia kudriavzevii 10023 SRR10406143 No Pichia kudriavzevii 10024 SRR10406151 No Pichia kudriavzevii 10025 SRR10406142 No Pichia kudriavzevii 10026 SRR10406156 No Pichia kudriavzevii 10027 SRR10406184 No Pichia kudriavzevii 10028 SRR10406183 No Pichia kudriavzevii 10029 SRR10406182 No Pichia kudriavzevii 10030 SRR10406157 No Pichia kudriavzevii 10031 SRR10413694 No Pichia kudriavzevii 10032 SRR10413692 No Pichia kudriavzevii 10033 SRR10413717 No Pichia kudriavzevii 10034 SRR10413690 No Pichia kudriavzevii 10036 SRR10413689 No Pichia kudriavzevii 10037 SRR10413916 No Pichia kudriavzevii 10038 SRR10413716 No Pichia kudriavzevii 10039 SRR10413691 No Pichia kudriavzevii 10040 SRR10413693 No Pichia kudriavzevii 10041 SRR10414461 No Pichia kudriavzevii 10042 SRR10414468 No Pichia kudriavzevii 10043 SRR10414466 No Pichia kudriavzevii 10044 SRR10414465 No Pichia kudriavzevii 10045 SRR10414467 No Pichia kudriavzevii 10048 SRR10415356 No Pichia kudriavzevii 10050 SRR10415359 No Pichia kudriavzevii 10051 SRR10415357 No Pichia kudriavzevii 10052 SRR10415354 No Pichia kudriavzevii 10054 SRR10415358 No Pichia kudriavzevii 10055 SRR10415355 No Pichia kudriavzevii 10143 SRR10846470 No Pichia kudriavzevii 10144 SRR10845531 No Pichia kudriavzevii 10145 SRR10849312 No Pichia kudriavzevii 10146 SRR10846617 No Pichia kudriavzevii 10147 SRR10904160 No Pichia kudriavzevii 10148 SRR10846469 No Pichia kudriavzevii 10149 SRR10849311 No Pichia kudriavzevii 10150 SRR10904153 No Pichia kudriavzevii 10151 SRR10846471 No Pichia kudriavzevii 10152 SRR10845523 No Pichia kudriavzevii 10153 SRR10846320 No Pichia kudriavzevii 10154 SRR10849313 No Pichia kudriavzevii 10155 SRR10846314 No Pichia kudriavzevii 10156 SRR10845555 No Pichia kudriavzevii 10157 SRR10846322 No Pichia kudriavzevii 10158 SRR10846323 No Pichia kudriavzevii 10159 SRR10846614 No Pichia kudriavzevii 10160 SRR10848433 No Pichia kudriavzevii K 19 1 SRR19696126 No Pichia kudriavzevii K 192 SRR19696125 No Pichia kudriavzevii K 193 SRR19696124 No No Pichia kudriavzevii K 201 SRR19696123 No No Pichia kudriavzevii K 202 SRR19696122 No No Pichia kudriavzevii K 203 SRR19696121 No No Pichia kudriavzevii KCKM 1001 GCA_021233995.1 No No Pichia kudriavzevii KMBL5774 GCA_001879685.1 No No Pichia kudriavzevii M1110A GCA_023629315.1 No No Pichia kudriavzevii M12 GCA_000286515.1 No No Pichia kudriavzevii M2002 GCA_023629595.1 No No Pichia kudriavzevii M-3 GCA_031179465.1 No No Pichia kudriavzevii M7004B GCA_023629375.1 No No Pichia kudriavzevii MCFLIP SRR18739954 No No Pichia kudriavzevii NBRC1279 GCA_018402865.1 No No Pichia kudriavzevii NBRC1664 GCA_018402885.1 No No Pichia kudriavzevii NCYC 2658 GCA_029581905.1 No No Pichia kudriavzevii NG7 GCA_002798095.1 No No Pichia kudriavzevii NRRL Y- 10940 GCA_032595395.1 No No Pichia kudriavzevii NRRL Y-5396 GCA_003705515.2 No No Pichia kudriavzevii NRRL YB-373 GCA_032595495.1 No No Pichia kudriavzevii NRRL YB-431 GCA_032595415.1 No No Pichia kudriavzevii NRRL YB-592 GCA_032595455.1 No No Pichia kudriavzevii NRRL YB-615 GCA_032595435.1 No No Pichia kudriavzevii Nuruk GCA_021014275.1 No No Pichia kudriavzevii P40-11 GCA_023629495.1 No No Pichia kudriavzevii P7006C GCA_023629475.1 No No Pichia kudriavzevii PB2809B GCA_025503585.1 No No Pichia kudriavzevii PM40-15 GCA_023629415.1 No No Pichia kudriavzevii S40-07 GCA_023629455.1 No No Pichia kudriavzevii SCHI0087. S.155 GCA_044592665.1 No No Pichia kudriavzevii SCHI0131. M.3 GCA_032359565.1 No No Pichia kudriavzevii SD108 GCA_000764455.1 No No Pichia kudriavzevii SJP GCA_003033855.1 No No Pichia kudriavzevii VPCI 1381-P-18 ERR7438469 No No Pichia kudriavzevii VPCI 1390-P-18 ERR7438470 No No Pichia kudriavzevii VPCI 1461-P-18 ERR7438471 No No Pichia kudriavzevii VPCI 1483-P-18 ERR7438466 No No Pichia kudriavzevii VPCI 1596-P-18 ERR7438467 No No Pichia kudriavzevii VPCI 1734-P-18 ERR7438468 No No Pichia kudriavzevii VPCI 494-P-18 ERR7438464 No No Pichia kudriavzevii VPCI 506-P-15 ERR7438465 No No Pichia kudriavzevii X2809A GCA_023629515.1 No No Pichia kudriavzevii Y1104A GCA_023629615.1 No No Pichia kudriavzevii Y114-04A GCA_037042275.1 No No Pichia kudriavzevii Y4012 GCA_023629355.1 No No Pichia kudriavzevii Y5A GCA_025503635.1 No No Pichia kudriavzevii YFJ002-604 ERR7438462 No No Pichia manshurica B7015 GCA_025531645.1 No No Pichia manshurica F8012 GCA_025531675.1 No No Pichia manshurica KUST-F1705 GCA_030769885.1 No No Pichia manshurica NRRL Y-27978 GCA_030220005.1 No No Pichia manshurica NRRL Y-27978T GCA_030578975.1 No No Pichia manshurica P8010B GCA_025531665.1 No No Pichia manshurica Y1106A GCA_025531695.1 No No Pichia manshurica YM63 GCA_005406165.1 No No Pichia membranifaciens KS47-1 GCA_001950575.1 No No Pichia membranifaciens NCYC 55 GCA_031021045.1 No No Pichia membranifaciens NRRLY-2026 GCA_001661235.1 No No Pichia nakasei CLIB 1057 ERR9707005 No No Pichia nakasei CLIB 976 ERR9707006 No No Pichia nakasei NRRL Y-7686 GCA_003705495.1 No No Pichia nanzhaoensis CBS 15346 GCA_030573035.1 No No Pichia norvegensis CBS 1922 GCA_003339405.1 No No Pichia norvegensis NRRLY-7687 GCA_030570285.1 No No Pichia occidentalis ApC GCA_030142935.1 No No Pichia occidentalis CLIB 3294 ERR9706983 No No Pichia occidentalis F2013-T GCA_023629695.1 No No Pichia occidentalis NRRLY-6545 GCA_029581965.1 No No Pichia occidentalis NRRLY-7552 GCA_030579475.1 No No Pichia occidentalis NRRLYB-3389 GCA_029581915.1 No No Pichia occidentalis PH4030B GCA_023629655.1 No No Pichia occidentalis UCDFST 75-63 GCA_029581985.1 No No Pichia paraexigua CBS 15237 GCA_030573095.1 No No Pichia pseudocactophila CBS 6929 GCA_039700735.1 No No Pichia pseudocactophila NRRL Y-17239 GCA_030580035.1 No No Pichia pseudolambica (form. C. pseudolambica) NRRLY- No No 17318T GCA_030569595.1

[0517] Pichia rugopelliculosa (form. C. rugopelliculosa) NRRL Y- No No 17079 GCA_030570335.1

[0518] Pichia scutulata NRRL Y-7663 GCA_030560845.1 No No Pichia sp. F1605B GCA_025531535.1 No No Pichia sp. KDB- 2024a GCA_040437495.1 No No Pichia sp. NRRLY-12824 GCA_030560915.1 No No Pichia sp. NRRLY-12827 GCA_030570315.1 No No Pichia sp. NRRLY-12830 GCA_030564565.1 No No Pichia sp. NRRLY-27259 GCA_030563085.1 No No Pichia sp. NRRLY-27261 GCA_030569835.1 No No Pichia sp. NRRLYB-4149 GCA_030556025.1 No No Pichia sp. P1605 GCA_025531595.1 No No Pichia sp. P7020A GCA_025531525.1 No No Pichia sp. PH4023 GCA_025531575.1 No No Pichia sp. PX1907B GCA_025531625.1 No No Pichia sp. VM-2024a GCA_040938015.1 No No Pichia sp. yHKS168 GCA_030571145.1 No No Pichia sp. yHMH446 GCA_030555725.1 No No Pichia sporocuriosa NRRL Y-27347 GCA_030574275.1 No No Pichia terricola 0621 GCA_030168465.1 No No Pichia terricola M2015 GCA_023629215.1 No No Pichia terricola M2019 GCA_023629275.1 No No Pichia terricola NRRL YB-4310 GCA_030581875.1 No No Pichia terricola P1002 GCA_023629295.1 No No Pichia terricola P2008 GCA_023629235.1 No No Pichia terricola PG8608 GCA_037041655.1 No No Pichia terricola WJL-G4 GCA_022701355.1 No No Pichia thaimueangensis (form. C. thaimueangensis) NBRC

[0519] No No 101967 GCA_030569635.1

[0520] Saturnispora saitoi (form. P. saitoi) NRRL Y-6671 SRR6476045 No No Pichia kudriavzevii 20058T SRR18739960 No No 8 Yamadazyma nakazawae (form. P. nakazawae) NRRL Y-7903 SRR6475911 No No Yamadazyma philogaea (form. P. philogaea) NRRL Y-7813 SRR6475886 No No

[0521] 1C. parapsilosis amplicon is within a small assembly contig (801bp). This contig has 100% identity to other C. parapsilosis strains, but not any N. glabratus strains. Genome is also ~4Mbp larger than other N. glabratus, suggesting contamination with other species

[0522] 2Low coverage data for this clinical strain at the amplicon site. ~50% reads have a single bp SNP in the probe-binding region, and ~50% match our probe. May be a gene duplication

[0523] 3Reassigned to Candida parapsilosis based on BLAST analysis of assembled genome

[0524] 4Reassigned to Candida albicans based on BLAST analysis of assembled genome

[0525] 5Reassigned to Candida orthopsilosis based on BLAST analysis of assembled genome

[0526] 6Incorrectly assigned as Candida albicans

[0527] 7Incorrectly assigned as Candida dubliniensis

[0528] 8Reassigned to Wickerhamomyces anomalus based on BLAST analysis of assembled genome Table 5: In vitro q PCR amplification of yeast and filamentous fungal DNA with N. glabratus, P. kudriavzevii, C. albicans, C. dubliniensis, C. parapsilosis, and C. tropicalis assays

[0529]

[0530]

[0531]

[0532] 87. S.1 Cryptococcus neoformans NA NA NA NA NA NA

[0533] 87. S.119 Curvularia lunata NA NA NA NA NA NA

[0534] 87. S.114 Curvularia mebaldsii NA NA NA NA NA NA

[0535] 87. S.115 Curvularia platzii NA NA NA NA NA NA

[0536] 87. S.134 Diaporthe batatas NA NA NA NA NA NA

[0537] 87. S.126 Diaporthe charlesworthii NA NA NA NA NA NA

[0538] 87. S.121 Diplodia africana NA NA NA NA NA NA

[0539] 87. S.120 Elsinoe australis NA NA NA NA NA NA

[0540] 87. S.8 Exophiala spinifera NA NA NA NA NA NA

[0541] 181. S.3d Fungus NA NA NA NA NA NA

[0542] 181. S.1e Penicillium oxalicum NA NA NA NA NA NA

[0543] 87. S.128 Fungus NA NA NA NA NA NA

[0544] 87. S.18 Fungus NA NA NA NA NA NA

[0545] 87. S.46 Fungus NA NA NA NA NA NA

[0546] 87. S.135 Neocosmospora falciformis NA NA NA NA NA NA

[0547]

[0548] 87. S.118 Fusarium gibbosum NA NA NA NA NA NA 87. S.143 Gaeumannomyces graminicola NA NA NA NA NA NA

[0549] 87. S.154 Geotrichum candidum NA NA NA NA NA NA

[0550] 76.S.1 Hanseniaspora opuntiae NA NA NA NA NA NA

[0551] 87. S.137 Linnemania elongata NA NA NA NA NA NA

[0552] 87. S.7 Lomentospora prolificans NA NA NA NA NA NA

[0553] 87. S.133 Meira argovae NA NA NA NA NA NA

[0554] 87. S.147 Metarhizium rileyi NA NA NA NA NA NA

[0555] 87. S.124 Microthia havanensis NA NA NA NA NA NA

[0556] 137. M.1 Nakaseomyces glabratus Yes - confirmed N. glabratus 20.14 NA NA NA NA NA

[0557] 39.5.2 Nakaseomyces glabratus 24.52 NA NA NA NA NA

[0558] 74.5.2 Nakaseomyces glabratus 25.85 NA NA NA NA NA

[0559] 87. S.12 Nakaseomyces glabratus 21.61 NA NA NA NA NA

[0560]

[0561] *The N. glabratus SEQ ID NO:3 probe (using SEQ NO:1 and NO:2 primers), when run in duplex with P. kudriavzevii SEQ ID NO:7 probe (using SEQ ID NO:5 and NO:6 primers), generates low-level pull-up in P. kudriavzevii isolates that can be safely ignored. This pull-up does not occur when using the N. glabratus-P. kudriavzevii duplex format comprising SEQ IDs NO: 1, NO:2, NO:4, NO:5, NO:6, and NO:8.

Claims

CLAIMS1. An isolated nucleic acid comprising, consisting essentially of, or consisting of a nucleotide sequence selected from the group consisting of:(i) any one of SEQ ID NOS: 1-28;(ii) a nucleotide sequence complementary to any of the nucleotide sequences in (ii);(iii) a nucleotide sequence having at least 70% nucleotide sequence to any of the nucleotide sequences in (i) or (ii); and(iv) a fragment of the isolated nucleic acid of any one of (i)-(iii).

2. The isolated nucleic acid of Claim 1, wherein the isolated nucleic acids of SEQ, ID NOS: SEQ ID NOS: 1, 2, 5, 6, 9, 10, 12, 13, 15, 16, 19 and 20 are primers for nucleic acid sequence amplification of yeast target nucleic acids,3. The isolated nucleic acid of Claim 1 or Claim 2, wherein the yeast target nucleic acid includes: a Nakaseomyces glabratus target nucleic acid; a Pichia kudriavzevii target nucleic acid; a Candida tropicalis target nucleic acid; a Candida aibicans target nucleic acid; a Candida parapsilosis target nucleic acid; and / or a Candida dubiiniensis target nucleic acid.

4. The isolated nucleic acid of Claim 1 or Claim 2 or Claim 3, wherein: the nucleotide sequences of SEQ ID NOS: 1 and 2 are respective forward and reverse primers suitable for nucleic acid sequence amplification of a Nakaseomyces glabratus target nucleic acid; the nucleotide sequences of SEQ ID NOS: 5 and 6 are respective forward and reverse primers suitable for nucleic acid sequence amplification of a Pichia kudriavzevii target nucleic acid; the nucleotide sequences of SEQ ID NOS: 9 and 10 are respective forward and reverse primers suitable for nucleic acid sequence amplification of a Candida tropicalis target nucleic acid; the nucleotide sequences of SEQ ID NOS:12 and 13 are respective forward and reverse primers suitable for nucleic acid sequence amplification of a Candida albicans target nucleic acid; the nucleotide sequences of SEQ ID NOS: 15 and 16 are respective forward and reverse primers suitable for nucleic acid sequence amplification of a Candida parapsilosis target nucleic acid; and the nucleotide sequences of SEQ ID NOS: 19 and 20 are respective forward and reverse primers suitable tornucleic acid sequence amplification of a Candida dubliniensis target nucleic acid.

5. The isolated nucleic acid of Claim 4, wherein the N. glabratus target nucleic acid encodes the Sip3p transcription cofactor in ATCC 2001 chr J (GI51_J07799, 822329..822600); the P. kudriavzevii target nucleic acid encodes Chain A, Autophagy-related protein 18 in CY902 chromosome I (unannotated, 1184190..1184309); the C. tropicalis target nucleic add is an unannotated region in MYA-3404 chr 3 (CP047871.1, 1205838..1205956); the C. albicans target nucleic acid is an unannotated region in SC5314 chr 1 (CP017623.1, 1562242..1562356); the C. parapsilosis target nucleic acid is CDC317 annotated contig 005504 (HE605202.1, 1028,.1195) uncharacterised locus; and the C. dubliniensis target nucleic acid is CD36 chr 1 (FM992688.1, 1596149..1596263) uncharacterised locus.

6. The isolated nucleic acid of any preceding claim, wherein the isolated nucleic acids of SEQ ID NOS: 3, 4, 7, 8, 11, 14, 17, 18, 21 and 22 are probes.

7. The isolated nucleic acid of Claim 6, wherein: probes comprising the nucleotide sequences of SEQ ID NOS: 3 and / or 4 are capable of hybridizing to an amplification product produced by nucleic acid sequence amplification with primers according to SEQ ID NOS: 1 and 2; probes comprising the nucleotide sequences of SEQ ID NOS: 7 and / or 8 are capable of hybridizing to an amplification product produced by nucleic acid sequence amplification with primers according to SEQ ID NOS: 5 and 6; a probe comprising the nucleotide sequence of SEQ ID NO: 11 is capable of hybridizing to an amplification product produced by nucleic acid sequence amplification with primers according to SEQ ID NOS: 9 and 10; a probe comprising the nucleotide sequence of SEQ ID NO: 14 is capable of hybridizing to an amplification product produced by nucleic acid sequence amplification with primers according to SEQ ID NOS: 12 and 13; probes comprising the nucleotide sequences of SEQ ID NOS: 17 and / or 18 are capable of hybridizing to an amplification product produced by nucleic acid sequence amplification with primers according to SEQ ID NOS: 15 and 16; and / or probes comprising the nucleotide sequences of SEQ ID NOS: 21 and / or 22 are capable of hybridizingto an amplification product produced by nucleic acid sequence amplification with primers according to SEQ ID NOS: 19 and 20.

8. The isolated nucleic acid of Claim 6 or Claim 7, wherein: probes comprising respective nucleotide sequences SEQ ID NOS: 3 and 4 can each hybridize to an amplification product comprising the nucleotide sequence set forth in SEQ ID NO:23; probes comprising respective nucleotide sequences set forth in SEQ ID NOS: 7 and 8 can each hybridize to an amplification product comprising the nucleotide sequence set forth in SEQ ID NO:24; a probe comprising a nucleotide sequence set forth in SEQ ID NO:11 can hybridize to an amplification product comprising the nucleotide sequence set forth in SEQ ID NO:25; a probe comprising a nucleotide sequence set forth in SEQ ID NO:14 can hybridize to an amplification product comprising the nucleotide sequence set forth in SEQ ID NO:26; probes comprising respective nucleotide sequences SEQ ID NOS: 17 and 18 can each hybridize to an amplification product comprising the nucleotide sequence set forth in SEQ ID NO:27; and / or probes comprising respective nucleotide sequences set forth in SEQ ID NOS: 21 and 22 can each hybridize to an amplification product comprising the nucleotide sequence set forth in SEQ ID NO:28.

9. The isolated nucleic acid of any one of Claims 6-8, wherein the probes are labelled.

10. The isolated nucleic acid of Claim 9, wherein the probes are labelled as follows:(A) SEQ ID NO:3 - 5’-FAM or 5'-CAL Fluor Gold 540 and 3’-BHQ-l;(B) SEQ ID NO:4 - 5'-FAM and 3'-BHQ-1;(C) SEQ ID NO:7 - 5'-FAM and 3'-BHQ-1;( D) SEQ ID NO:8 - 5'-FAM and 3'-BHQ-l;(E) SEQ ID NO:11 - 5'-FAM and 3'-BHQ-1;(F) SEQ ID NO:14 - 5'-CAL Fluor Gold 540 and 3'-BHQ-l or 5' -CAL Fluor Red 610 and 3' -BHQ-2;(G) SEQ ID NO:17 - 5'-FAM and 3'-BHQ-1;(H) SEQ ID NO:18 - 5'-FAM and 3'-BHQ-1;Ill( ) SEQ ID NO:21 - 5'-CAL Fluor Gold 540 and 3'-BHQ-l or 5'-Quasar 670 and 3'-BHQ-2 and / or;(J) SEQ ID NO:22 - 5'-HEX and 3'-BHQ-1.

11. A method of amplifying a yeast target nucleic acid with one or more primers that respectively comprise, consist essentially of, or consist of the nucleotide sequences of any one or more of SEQ. ID NOS: 1,2, 5, 6, 9, 10, 12, 13, 15, 16, 19 and / or 20, or that respectively comprise, consist essentially of, or consist of a nucleotide sequence having at least 70% nucleotide sequence identity to any one of SEQ ID NOS: 1,2, 5, 6, 9, 10, 12, 13, 15, 16, 19 and / or 20, or a fragment thereof, to thereby produce an amplification product.

12. A method of detecting the presence of absence of a yeast target nucleic acid, said method including the step of subjecting a nucleic acid sample to nucleic acid sequence amplification with primers respectively comprising, consisting essentially of, or consisting of nucleotide sequences set forth in any one of more of SEQ ID NOS: 1,2, 5, 6, 9, 10, 12, 13, 15, 16, 19 and / or 20, or that respectively comprise, consist essentially of, or consist of a nucleotide sequence having at least 70% nucleotide sequence identity to any one of SEQ ID NOS: 1,2, 5, 6, 9, 10, 12, 13, 15, 16, 19 and / or 20, or a fragment thereof, wherein the presence of an amplification product indicates that the yeast target nucleic acid is present in said sample and an absence of an amplification product indicates that the yeast target nucleic acid is absent from said sample.

13. The method of Claim 11 or Claim 12, wherein the yeast target nucleic acid includes: a Nakaseomyc.es glabratus target nucleic acid; a Pichia kudriavzevii target nucleic acid; a Candida tropicalis target nucleic acid; a Candida albicans target nucleic acid; a Candida parapsilosis target nucleic acid; and / or a Candida dubliniensis target nucleic acid.

14. The method of Claim 13, wherein the N. glabratus target nucleic acid encodes the Sip3p transcription cofactor in ATCC 2001 chr J (GVI51_J07799, 822329..822600); the P. kudriavzevii target nucleic acid encodes Chain A, Autophagy-related protein 18 in CY902 chromosome I (unannotated, 1184190..1184309); the C. tropicalis target nucleic acid is an unannotatedregion in MYA-3404 chr 3 (CP047871.1, 1205838..1205956); the C. albicans target nucleic acid is an unannotated region in SC5314 chr 1 (CP017623.1, 1562242..1562356); the C. parapsilosis target nucleic acid is CDC317 annotated contig 005504 (HE605202.1, 1028..1195) uncharacterised locus; and the C. dubliniensis target nucleic acid is CD36 chr 1 (FM992688.1, 1596149..1596263) uncharacterised locus.

15. A method of detecting the presence of absence of a yeast target nucleic acid, said method including the step of subjecting a nucleic acid sample to nucleic acid sequence amplification with primers to thereby produce one or more amplification products that respectively comprise, consist essentially of, or consist of a nucleotide sequence having at least 70% nucleotide sequence identity to any one of SEQ ID NOS:23-28, or a fragment thereof, wherein the presence of an amplification product indicates that the yeast target nucleic acid is present in said sample and an absence of an amplification product indicates that the yeast target nucleic acid is absent from said sample.

16. The method of any one of Claims 10-15, including the step of hybridizing one or more probes to one or more of the respective amplification products.

17. The method of Claim 16, wherein the probes comprise nucleotide sequences selected from the group consisting of SEQ ID NOS: 3, 4, 7, 8, 11, 14, 17, 18, 21 and / or 22.

18. The method of Claim 17, wherein: probes comprising the nucleotide sequences of SEQ. ID NOS: 3 and / or 4 hybridize to an amplification product produced by nucleic acid sequence amplification with primers according to SEQ, ID NOS: 1 and 2; probes comprising the nucleotide sequences of SEQ. ID NOS: 7 and / or 8 hybridize to an amplification product produced by nucleic acid sequence amplification with primers according to SEQ ID NOS: 5 and 6; a probe comprising the nucleotide sequence of SEQ ID NO: 11 hybridizes to an amplification product produced by nucleic acid sequence amplification with primers according to SEQ ID NOS: 9 and 10; a probe comprising the nucleotide sequence of SEQ ID NO: 14 hybridizes to an amplification product produced by nucleic acid sequence amplification with primers according to SEQ ID NOS: 12 and 13; probes comprising the nucleotide sequences of SEQ ID NOS: 17and / or 18 hybridize to an amplification product produced by nucleic acid sequence amplification with primers according to SEQ ID NOS: 15 and 16; and / or probes comprising the nucleotide sequences of SEQ ID NOS: 21 and / or 22 hybridize to an amplification product produced by nucleic acid sequence amplification with primers according to SEQ ID NOS: 19 and 20.

19. The isolated nucleic acid of Claim 17 or Claim 18, wherein: probes comprising respective nucleotide sequences SEQ ID NOS: 3 and / or 4 hybridize to an amplification product comprising the nucleotide sequence set forth in SEQ ID NO:23; probes comprising respective nucleotide sequences set forth in SEQ ID NOS: 7 and / or 8 hybridizes to an amplification product comprising the nucleotide sequence set forth in SEQ ID NO:24; a probe comprising a nucleotide sequence set forth in SEQ ID NO:11 hybridizes to an amplification product comprising the nucleotide sequence set forth in SEQ ID NO:25; a probe comprising a nucleotide sequence set forth in SEQ ID NO:14 hybridizes to an amplification product comprising the nucleotide sequence set forth in SEQ ID NO:26; probes comprising respective nucleotide sequences SEQ ID NOS: 17 and / or 18 hybridize to an amplification product comprising the nucleotide sequence set forth in SEQ ID NO:27; and / or probes comprising nucleotide sequences set forth in SEQ ID NOS: 21 and / or 22 hybridize to an amplification product comprising the nucleotide sequence set forth in SEQ ID NO:28.20 The method of any one of Claims 16-19, wherein the probes are labelled.

21. The method of Claim 20 wherein the probes are labelled as follows:(A) SEQ ID NO:3 - 5'-FAM or 5'-CAL Fluor Gold 540 and 3'-BHQ-l;(B) SEQ ID NO:4 - 5'-FAM and 3'-BHQ-1;(C) SEQ ID NO:7 - 5'-FAM and 3'-BHQ-1;(D) SEQ ID NO:8 - 5'-FAM and 3'-BHQ-1;(E) SEQ ID NO:11 - 5'-FAM and 3'-BHQ-1;(F) SEQ ID NO:14 - 5'-CAL Fluor Gold 540 and 3'-BHQ-l or 5'-CAL Fluor Red 610 and 3'-BHQ-2;(G) SEQ ID NO:17 - 5'-FAM and 3'-BHQ-1;(H) SEQ ID NO:18 - 5'-FAM and 3'-BHQ-1;( ) SEQ ID NO:21 - 5'-CAL Fluor Gold 540 and 3'-BHQ-l or 5'-Quasar 670 and 3'-BHQ-2 and / or;(J) SEQ ID NO:22 - 5'-HEX and 3'-BHQ-1.

22. A method of treating or preventing yeast colonization, infection or infestation, said method including the steps of detecting the presence of a yeast nucleic acid according to the method of any one of Claims 10-21 and; treating the yeast infection or infestation to thereby at least partly prevent, remove, eliminate or otherwise alleviate the yeast colonization, infection or infestation,23. A method of determining the ability of one or more primers to amplify a nucleic acid, said method including the step of comparing the respective nucleotide sequences of one or more primers respectively comprising, consisting essentially of, or consisting of nucleotide sequences set forth in any one of more of SEQ. ID NOS: 1,2, 5, 6, 9, 10, 12, 13, 15, 16, 19 and / or 20, or that respectively comprise, consist essentially of, or consist of a nucleotide sequence having at least 70% nucleotide sequence identity to any one of SEQ ID NOS: 1,2, 5, 6, 9, 10, 12, 13, 15, 16, 19 and / or 20, or a fragment thereof, with a nucleotide sequence of the nucleic acid to thereby determine the ability of the one or more primers to amplify the nucleic acid.

24. The method of Claim 23, wherein the step of comparing the respective nucleotide sequences of the one or more primers with the nucleotide sequence of the nucleic acid is performed in silico.

25. A kit comprising one or more isolated nucleic acid primers or probes comprising nucleotide sequences according to SEQ ID NOS:l-28, or comprising a nucleotide sequence at least 70% identical thereto or a fragment thereof, and optionally one or more other components selected from the group consisting of: a thermostable DNA polymerase; a reverse transcriptase; and one or more detection reagents.

26. The kit of Claim 25, wherein the probes are labelled as follows:(A) SEQ ID NO:3 - 5'-FAM or 5'-CAL Fluor Gold 540 and 3'-BHQ-l;(B) SEQ ID NO:4 - 5'-FAM and 3'-BHQ-1;(C) SEQ ID NO:7 - 5'-FAM and 3'-BHQ-1;(D) SEQ ID N0:8 - 5'-FAM and 3'-BHQ-1;(E) SEQ. ID NO:11 - 5'-FAM and 3'-BHQ-l;(F) SEQ ID N0:14 - 5'-CAL Fluor Gold 540 and 3'-BHQ-l or 5'-CAL Fluor Red 610 and 3'-BHQ-2;(G) SEQ ID NO:17 - 5'-FAM and 3'-BHQ-l;(H) SEQ ID NO:18 - 5'-FAM and 3'-BHQ-l;( ) SEQ ID NO:21 - 5'-CAL Fluor Gold 540 and 3'-BHQ-l or 5'-Quasar 670 and 3'-BHQ-2 and / or;(J) SEQ ID NO:22 - 5'-HEX and 3'-BHQ-1.

27. The kit of Claim 25 or Claim 26, when used according to the method of any one of Claims 10-24.

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