Method and device for detecting a plurality of nucleic acids in parallel

By employing fluorescent dyes with different melting curve temperatures and molecular beacons, the method addresses the challenge of cross-talk in nucleic acid detection, enabling efficient and accurate multiplexed nucleic acid analysis.

WO2026125714A1PCT designated stage Publication Date: 2026-06-18ROBERT BOSCH GMBH
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Patent Information

Authority / Receiving Office
WO · WO
Patent Type
Applications
Current Assignee / Owner
ROBERT BOSCH GMBH
Filing Date
2025-12-12
Publication Date
2026-06-18

AI Technical Summary

⚠Technical Problem

Existing methods for simultaneously detecting multiple nucleic acids face challenges in distinguishing between nucleic acid-binding fluorescent dyes due to overlapping excitation and emission wavelengths, leading to cross-talk and reduced spectral distinguishability.

⚗Method used

Utilizing fluorescent dyes with distinct melting curve temperatures and molecular beacons with different hairpin structures to selectively excite and detect multiple nucleic acids, employing a method that involves a melting curve analysis to thermally exclude certain dyes based on their melting temperatures, thereby reducing cross-talk and allowing for more efficient multiplexing.

🎯Benefits of technology

Enables reliable and efficient detection of multiple nucleic acids by minimizing cross-talk and optimizing the use of optical channels, allowing for higher throughput in nucleic acid detection.

✦ Generated by Eureka AI based on patent content.

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Abstract

The invention relates to a method for detecting a plurality of nucleic acids in parallel. The method comprises carrying out an amplification (42 - 44) of the nucleic acids in the presence of a plurality of nucleic-acid-binding fluorescent dyes, and carrying out a melting curve analysis (52 - 55) of the amplification product with a first group of optical channels. A plurality of first fluorescent dyes have melting curve temperatures which are below the melting curve temperatures of a plurality of second fluorescent dyes. The invention also relates to a device for detecting a plurality of nucleic acids in parallel. The device is designed to carry out the steps of the method.
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