Gas detection sensor comprising aminated graphene and peptide, analysis device, and gas analysis method

Abstract

The present invention relates to a gas detection sensor comprising two or more gas detection segments, a gas analysis device, and a gas analysis method using same. The type of gas included in mixed gas can be analyzed using the two or more gas detection segments, and the gas can be analyzed with excellent sensitivity and selectivity through vector signaling and imaging steps.

Description

Gas sensing sensor, analysis device, and gas analysis method comprising aminized graphene and peptide

[0001] The present invention relates to a gas detection sensor, a gas analysis device, and a gas analysis method.

[0002]

[0003] In modern society, the importance of gas analysis devices and methods is increasingly emphasized in various fields, such as air pollution, industrial process safety, environmental monitoring, and medical diagnosis. These gas analysis devices are used to precisely monitor environmental changes or selectively detect specific compounds, playing a crucial role in detecting changes in hazardous gas concentrations, tracking chemical reactions, and real-time diagnosis.

[0004] However, existing gas analysis devices and methods have limitations in detecting minute concentrations of gas or lack selectivity in distinguishing individual gas types within mixed gases; they often operate stably only under specific temperature or humidity conditions, or their sensor performance is frequently sensitive to changes in the external environment.

[0005] Accordingly, there is a need to develop new gas analysis technologies that have high gas recognition sensitivity, exhibit selective reactions to specific compounds, and can operate stably under various environmental conditions.

[0006] Recently, research is actively underway to solve these problems by utilizing new materials such as aminized graphene and peptide-based sensors.

[0007] These new materials have the advantage of superior selectivity and sensitivity compared to existing metal oxide-based technologies, as well as stable operation at room temperature.

[0008] The present invention aims to solve the technical problem of overcoming the limitations of existing gas analysis devices and methods and providing a gas detection sensor with high sensitivity and selectivity and a gas analysis method based thereon.

[0009] [Prior Art Literature]

[0010] [Patent Literature]

[0011] (Patent Document 1) Korean Registered Patent Publication No. 10-1191543 (October 15, 2012)

[0012]

[0013] The objective of the present invention to solve the problems of the prior art is to provide a gas detection sensor, a gas analysis device, and a gas analysis method.

[0014] Specifically, a gas detection sensor and a gas analysis device including the same include two or more gas detection segments, and a gas analysis method using the same is intended to enable the analysis of the type of gas included in the mixed gas through vector signaling and imaging processes.

[0015] However, the problems that the present invention aims to solve are not limited to those mentioned above, and other problems not mentioned but intended to be solved will be clearly understood by those skilled in the art to which the present invention belongs from the content to be described below.

[0016]

[0017] One aspect of the present invention is an insulating substrate (1);

[0018] Strip electrode (2); and

[0019] It includes at least 2 gas detection segments (0), and

[0020] The strip electrode (2) is formed on the insulating substrate (1), and

[0021] The above at least two gas sensing segments (0) are formed on the insulating substrate (1) and the strip electrode (2).

[0022] It provides a gas detection sensor.

[0023] Another aspect of the present invention is a method for manufacturing a gas detection sensor,

[0024] A step of depositing the strip electrode (2) on the insulating substrate (1);

[0025] A step of applying a suspension containing the aminized graphene onto the insulating substrate (1) and the strip electrode (2); and

[0026] The method comprises the step of manufacturing at least two gas sensing segments (0) by binding the above peptide to the above aminated graphene.

[0027] A method for manufacturing a gas detection sensor is provided.

[0028] Another aspect of the present invention is that the gas detection sensor comprises the above-mentioned gas detection sensor.

[0029] Provides a gas analysis device.

[0030] Another aspect of the present invention is a first step of collecting respective measurement values ​​from at least two gas detection segments (0) of a gas detection sensor according to any one of claims 1 to 16 before and after gas inflow;

[0031] A second step of filtering to exclude non-informative values ​​from each of the above measurements;

[0032] A third step of vectorizing the distribution of the filtered measurements across the entire strip electrode (2) below at least two gas detection segments (0);

[0033] A fourth step of converting the distribution of the filtered measurements vectorized above into vector data; and

[0034] A fifth step of plotting clusters representing the vector data of the gas in a reduced artificial space of features and quantifying the characteristics of the gas using Mahalanobis metrics;

[0035] The above-mentioned fourth step is performed by Principal Component Analysis (PCA), Linear Discriminant Analysis (LDA), correlation analysis, artificial neural network methods, or a combination thereof,

[0036] Provides a gas analysis method.

[0037]

[0038] According to the gas detection sensor, gas analysis device, and gas analysis method of the present invention, it is possible to analyze what type of gas is mixed in a mixed gas with excellent sensitivity and selectivity, and the gas analysis device can be operated stably at room temperature.

[0039]

[0040] FIGS. 1(a) and FIGS. 1(b) are a photograph and a plan view of a gas detection sensor, respectively, and FIGS. 1(c) shows a cross-section cut vertically along line AA of FIGS. 1(b).

[0041] Figure 2 is a schematic diagram of the process for manufacturing aminized graphene powder.

[0042] Figure 3 illustrates the process of preparing a suspension containing aminated graphene, applying it onto an insulating substrate and a strip electrode, and binding a peptide to the aminated graphene.

[0043] FIGS. 4(a) to 4(c) are electron microscope photographs of cross-sections of the first gas sensing segment (5) to the third gas sensing segment (7) manufactured in Manufacturing Example 2, respectively.

[0044] Figure 5 is a graph of amine graphene observed using X-ray Photoelectron Spectroscopy (XPS).

[0045] Figures 6(a) and 6(b) are graphs of the nitrogen 1s and carbon 1s spectra of Figure 5, respectively, observed at high resolution using X-ray photoelectron spectroscopy (XPS).

[0046] FIGS. 7(a) to 7(c) are graphs of the first gas detection segment (5) to the third gas detection segment (7), respectively, observed using X-ray photoelectron spectroscopy (XPS).

[0047] FIGS. 8(a) to 8(c) are graphs showing resistance values ​​according to exposure time when the first gas detection segment (5) to the third gas detection segment (7) are exposed to methanol, respectively.

[0048] FIGS. 9(a) to 9(c) are graphs showing resistance values ​​according to exposure time when the first gas detection segment (5) to the third gas detection segment (7) are exposed to ethanol, respectively.

[0049] FIGS. 10(a) to FIGS. 10(c) are graphs showing resistance values ​​according to exposure time when the first gas detection segment (5) to the third gas detection segment (7) are exposed to acetone, respectively.

[0050] FIGS. 11(a) to FIGS. 11(c) are graphs showing resistance values ​​according to exposure time when the first gas detection segment (5) to the third gas detection segment (7) are exposed to ammonia.

[0051] FIGS. 12(a) to 12(c) are graphs showing resistance values ​​according to exposure time when the first gas detection segment (5) to the third gas detection segment (7) are exposed to water vapor.

[0052] FIG. 13 is a graph showing the current according to the voltage when the first gas detection segment (5) to the third gas detection segment (7) are exposed to dry air at 25°C.

[0053] FIG. 14 is a schematic diagram of a device connected to a gas analysis device of Example 1 that can introduce a mixed gas.

[0054] FIGS. 15(a) to 15(c) are graphs measuring the chemical resistance response according to exposure time when the first gas sensing segment (5) to the third gas sensing segment (7) are exposed to methanol, respectively.

[0055] FIGS. 16(a) to 16(c) are graphs measuring the chemical resistance response according to exposure time when the first gas sensing segment (5) to the third gas sensing segment (7) are exposed to ethanol, respectively.

[0056] FIGS. 17(a) to 17(c) are graphs measuring the chemical resistance response according to exposure time when the first gas sensing segment (5) to the third gas sensing segment (7) are exposed to acetone, respectively.

[0057] FIGS. 18(a) to 18(c) are graphs measuring the chemical resistance response according to exposure time when the first gas sensing segment (5) to the third gas sensing segment (7) are exposed to ammonia, respectively.

[0058] FIGS. 19(a) to 19(c) are graphs measuring the chemical resistance response according to exposure time when the first gas sensing segment (5) to the third gas sensing segment (7) are exposed to water vapor, respectively.

[0059] Figure 20 is a graph showing a mixed gas of air, methanol (CH3OH), ethanol (C2H5OH), acetone (CH3COCH3), ammonia (NH3), and water vapor (H2O) in three dimensions after vector signaling and three-dimensional imaging using a gas analysis device.

[0060]

[0061] Hereinafter, the operation and effects of the invention will be described in more detail through specific embodiments and drawings. However, these embodiments are merely examples of the invention and do not define the scope of the invention.

[0062] Prior to this, terms and words used in this specification and claims should not be interpreted as being limited to their ordinary or dictionary meanings, but should be interpreted in a meaning and concept consistent with the technical spirit of the invention, based on the principle that the inventor can appropriately define the concept of the terms to best describe his invention.

[0063] Therefore, it should be understood that the configuration of the embodiments described in this specification is merely one of the most preferred embodiments of the present invention and does not represent all of the technical ideas of the present invention, and that various equivalents and modifications that can replace them may exist at the time of filing this application.

[0064] In this specification, singular expressions include plural expressions unless the context clearly indicates otherwise. In this specification, terms such as “comprising,” “comprising,” or “having” are intended to specify the existence of the implemented features, numbers, steps, components, or combinations thereof, and should be understood as not precluding the existence or addition of one or more other features, numbers, steps, components, or combinations thereof.

[0065] Where various parameters in this specification are given as an enumeration of ranges, preferred ranges, preferred upper limits, and preferred lower limits, it should be understood that any pair of any upper range limit or preferred value and any lower range limit or preferred value specifically discloses all ranges formed by any pair of any upper range limit or preferred value, regardless of whether the range is disclosed separately.

[0066] Where a range of numerical values ​​is mentioned in this specification, unless otherwise described, the range is intended to include its endpoint and all integers and fractions within the range.

[0067] The scope of the present invention is not intended to be limited to specific values ​​mentioned when defining the scope.

[0068] In the present specification, "a to b" and "a~b" indicating numerical ranges are defined as ≥a and ≤b.

[0069] Embodiments of the present invention have been described in detail below, but the present invention is not limited thereto.

[0070]

[0071] A gas detection sensor according to one aspect of the present invention may include an insulating substrate (1), a strip electrode (2), and at least two gas detection segments (0), wherein the strip electrode (2) is formed on the insulating substrate (1), and the at least two gas detection segments (0) may be formed on the insulating substrate (1) and the strip electrode (2).

[0072] For example, the at least two gas sensing segments (0) may be formed from the insulating substrate (1) between the strip electrodes (2), may fill the space between the strip electrodes, and may be formed in a shape that covers the space between the strip electrodes (2) and the strip electrodes.

[0073] Additionally, each of the at least two gas detection segments (0) may be adjacent to each other.

[0074] At this time, the strip electrode (2) may be formed horizontally on the insulating substrate (1), and the at least two gas sensing segments (0) may be formed at the center of the insulating substrate (1) and the strip electrode (2), and each of the at least two gas sensing segments (0) may be formed vertically adjacent to each other.

[0075] In one embodiment, the insulating substrate (1) may include quartz, glass, ceramic, silicon oxide, or a combination thereof.

[0076] For example, the insulating substrate (1) may mean a silicon substrate, and may additionally include a silicon oxide layer on the insulating substrate (1).

[0077] In one embodiment, the width of the strip electrode (2) may be 1 μm to 100 μm.

[0078] For example, the width of the strip electrode (2) may be 10 μm to 90 μm, 20 μm to 80 μm, 30 μm to 70 μm, or 40 μm to 60 μm.

[0079] If the width of the strip electrode (2) exceeds the range of the present invention, it may not be sufficient to produce a stable and identifiable change in the resistance of at least two gas sensing segments (0).

[0080] If the width of the strip electrode (2) is less than that of the present invention, the current is not sufficiently supplied to the gas detection segment, so the resistance that changes when detecting gas may not be measured.

[0081] In one embodiment, the thickness of the strip electrode (2) may be 50 nm to 1,000 nm.

[0082] For example, the thickness of the strip electrode (2) may be 60 nm to 800 nm, 70 nm to 600 nm, 80 nm to 500 nm, 85 nm to 400 nm, 90 nm to 300 nm, or 95 nm to 200 nm.

[0083] If the thickness of the strip electrode (2) exceeds that of the present invention, the gas sensing segment formed on the strip electrode (2) may not be stably deposited.

[0084] If the thickness of the strip electrode (2) is lower than that of the present invention, the current is not sufficiently supplied to the gas detection segment, so the resistance that changes when detecting gas may not be measured.

[0085] In one embodiment, the number of strip electrodes (2) is 2 or more, and the distance between the strip electrodes (2) may be 1 μm to 100 μm.

[0086] For example, the number of the strip electrodes (2) may be 2 or more and 100 or less, 5 or more and 90 or less, 10 or more and 80 or less, 20 or more and 70 or less, 25 or more and 60 or less, 30 or more and 55 or less, or 35 or more and 50 or less.

[0087] For example, the distance between the strip electrodes (2) may be 10 μm to 90 μm, 20 μm to 80 μm, 30 μm to 70 μm, or 40 μm to 60 μm.

[0088] If the number of the strip electrodes (2) exceeds the present invention, the size of the gas sensing segment formed on the strip electrodes (2) may be formed excessively large, and the gas sensing segment may be damaged by heat as it approaches the heater (4).

[0089] If the number of the strip electrodes (2) is less than that of the present invention, the current is not sufficiently supplied to the gas detection segment, so the resistance that changes when detecting gas may not be measured.

[0090] If the distance between the strip electrodes (2) exceeds the present invention, the current is not sufficiently supplied to the gas detection segment, so the resistance changing when detecting gas may not be measured.

[0091] If the distance between the strip electrodes (2) is lower than the present invention, the distance between the strip electrodes (2) may be too close, and there may be a risk of short circuit between the electrodes.

[0092] In one embodiment, the at least two gas sensing segments (0) may include aminated graphene, and based on the total atomic percentage of the aminated graphene, 100 atomic percentages, the atomic percentage of the amine may be 7 atomic percentages or more and 20 atomic percentages or less.

[0093] For example, based on a total atomic percentage of 100 atomic percentages of the aminated graphene, the atomic percentage of the amine may be 7.5 atomic percentages or more and 18 atomic percentages or less, 8.0 atomic percentages or more and 16 atomic percentages or less, 8.5 atomic percentages or more and 14 atomic percentages or less, 8.8 atomic percentages or more and 13 atomic percentages or less, or 9.0 atomic percentages or more and 12 atomic percentages or less.

[0094] If the atomic percentage of amine is below the range of the present invention, a sufficient number of peptides cannot be immobilized, so the change in resistance and selectivity of gas detection may be significantly reduced.

[0095] If the atomic percentage of amine exceeds the range of the present invention, the resistance of the aminated graphene may become too high to measure due to induced disruption of the graphene layer.

[0096] In one embodiment, the aminated graphene can be combined with a peptide.

[0097] In the present invention, a peptide may mean a combination of amino acids, and the peptide may mean that the N-terminus (-NH2) and C-terminus (-COOH) of the amino acids are connected by a peptide bond.

[0098] At this time, the above amino acids are cysteine ​​(C), isoleucine (I), histidine (H), asparagine (N), proline (P), arginine (R), glutamine (Q), valine (V), phenylalanine (F), tryptophan (W), serine (S), aspartic acid (D), alanine (A), tyrosine (Y), leucine (L), methionine (M), lysine (K), glycine (G), glutamic acid (E), pyrrolysine (O), selenocysteine ​​(U), It may be hydroxyproline or a combination thereof.

[0099] In one embodiment, the peptide is Cysteine-Arginine-Glutamine-Valine-Phenylalanine (CRQVF), Cysteine-Isoleucine-Histidine-Asparagine-Proline (CIHNP), Tryptophan-Histidine-Valine-Serine-Cysteine ​​(WHVSC), Aspartic acid-Serine-Tryptophan-Alanine-Alanine-Aspartic acid-Isoleucine-Proline (DSWAADIP), Aspartic acid-Asparagine-Proline-Isoleucine-Glutamine-Alanine-Valine-Proline (DNPIQAVP), Tryptophan-Histidine-Tyrosine-Glutamine-Arginine-Proline-Leucine-Methionine-Proline-Valine-Serine-Isoleucine (WHYQRPLMPVSI), Lysine-Serine-Aspartic acid-Serine-Cysteine ​​(KSDSC), Leucine-Glycine-Phenylalanine-Aspartic Acid-Cysteine ​​(LGFDC),Asparagine-Glutamine-Leucine-Serine-Asparagine-Leucine-Serine-Phenylalanine-Serine-Aspartic acid-Leucine-Cysteine ​​(NQLSNLSFSDLC), Valine-Phenylalanine-Serine-Isoleucine-Leucine-Serine-Proline-Leucine-Proline-Leucine-Isoleucine-Isoleucine-Proline-Phenylalanine-Valine-Cysteine ​​(Valine-Phenylalanine-Serine-Isoleucine-Leucine-Serine-Proline-Leucine-Proline-Leucine-Proline-Isoleucine-Isoleucine-Proline-Phenylalanine-Valine-Cysteine, It may include VFSILSPLPLIIPFVC), cysteine-isoleucine-glutamine-proline-valine (CIQPV), or a combination thereof.

[0100] For example, the peptide may be cysteine-arginine-glutamine-valine-phenylalanine (CRQVF), cysteine-isoleucine-histidine-asparagine-proline (CIHNP), tryptophan-histidine-valine-serine-cysteine ​​(WHVSC), or a combination thereof.

[0101] In one embodiment, the C-terminus of the peptide may be covalently bonded to the aminated graphene.

[0102] The carboxyl group of the above peptide can be combined with the aminated graphene via a highly reactive O-acylisourea, and the O-acylisourea can be generated by carbodiimide activation.

[0103] For example, the C-terminus of the proline in the cysteine-isoleucine-histidine-asparagine-proline peptide can covalently bond with the aminated graphene.

[0104] In one embodiment, the peptides included in each of the gas sensing segments of the at least two gas sensing segments (0) may be the same or different from each other.

[0105] In one embodiment, the at least two gas sensing segments (0) may include a first gas sensing segment (5), a second gas sensing segment (6), and a third gas sensing segment (7), wherein the peptide of the first gas sensing segment (5) comprises Cysteine-Arginine-Glutamine-Valine-Phenylalanine (CRQVF), the peptide of the second gas sensing segment (6) comprises Cysteine-Isoleucine-Histidine-Asparagine-Proline (CIHNP), and the peptide of the third gas sensing segment (7) comprises Tryptophan-Histidine-Valine-Serine-Cysteine ​​(WHVSC). It can be included.

[0106] At this time, the first gas detection segment (5), the second gas detection segment (6), and the third gas detection segment (7) may be adjacent.

[0107] For example, the first gas detection segment (5), the second gas detection segment (6), and the third gas detection segment (7) may be formed on the insulating substrate (1) and the strip electrode (2), and when the strip electrode (2) is formed horizontally on the insulating substrate (1), the first gas detection segment (5), the second gas detection segment (6), and the third gas detection segment (7) may be formed vertically adjacent to the center of the insulating substrate (1) and the strip electrode (2).

[0108] At this time, the first gas detection segment (5) may be formed at the top of the vertical direction, the second gas detection segment (6) may be formed adjacent to the first gas detection segment (5) and at the bottom of the first gas detection segment (5), and the third gas detection segment (7) may be formed at the bottom of the second gas detection segment (6).

[0109] For example, the first gas sensing segment (5) may have a peptide composed of cysteine-arginine-glutamine-valine-phenylalanine (CRQVF) covalently bonded to aminated graphene, the second gas sensing segment (6) may have a peptide composed of cysteine-isoleucine-histidine-asparagine-proline (CIHNP) covalently bonded to aminated graphene, and the third gas sensing segment (7) may have a peptide composed of tryptophan-histidine-valine-serine-cysteine ​​(WHVSC) covalently bonded to aminated graphene.

[0110] In one embodiment, the gas detection sensor can detect methanol (CH3OH), ethanol (C2H5OH), acetone (CH3COCH3), ammonia (NH3), water vapor (H2O), acetonitrile, ethyl acetate, nonanal, octanal, terpin-4-ol, ethylbutanoate, ethyl octanoate, hexane, trinitrotoluene, or a combination thereof.

[0111] The peptide included in the above gas detection sensor may exhibit a chemical resistance response upon gas exposure.

[0112] The peptide included in the above gas detection sensor can cause a chemical reaction when exposed to gas, and the resistance value may change as a type of chemical resistance response.

[0113] By measuring this chemical resistance response, the presence of gas can be detected in real time, and the type of gas can be distinguished by checking the chemical resistance response according to the type of peptide.

[0114] In one embodiment, the thickness of the at least two gas sensing segments (0) may be 5 nm to 100 nm.

[0115] For example, the thickness of the at least two gas sensing segments (0) may be 6 nm to 80 nm, 7 nm to 60 nm, or 8 nm to 50 nm.

[0116] If the thickness of at least two gas sensing segments (0) is lower than the range of the present invention, the space between the surface of the strip electrode (2) and the strip electrode may not be uniformly coated with aminated graphene with immobilized peptide, and the electrical resistance of the gas sensing segments (0) may be higher than 100 MΩ.

[0117] If the thickness of at least two gas sensing segments (0) exceeds the range of the present invention, the accessibility of the vapor of the analysis gas or analysis substance to the lower layer (volume) of the material in direct contact with the strip electrode (2) is reduced, and the sensitivity of the sensor may be significantly reduced.

[0118] In one embodiment, the average thickness of each of the at least two gas detection segments (0) may be the same or different.

[0119] When the average thickness of each of the at least two gas detection segments (0) is different, the chemical resistance value that changes due to gas is increased, and the accuracy of gas analysis may be higher compared to when the average thickness of the gas detection segments is the same.

[0120] For example, the average thickness of the first gas detection segment (5) to the third gas detection segment (7) may be 41 nm, 12 nm, and 28 nm, respectively.

[0121] In one embodiment, the thickness of each gas sensing segment may not be uniform.

[0122] For example, each of the above gas sensing segments may be in a slanted shape with one side gradually becoming thicker, or in an irregular shape.

[0123] In one embodiment, the ratio of the minimum thickness to the average thickness of each of the at least two gas detection segments (0) (the minimum thickness of each gas detection segment: the average thickness of each detection segment) may be 0.9 to 0.95:1, and the ratio of the maximum thickness to the average thickness of each of the at least two gas detection segments (0) (the maximum thickness of each gas detection segment: the average thickness of each detection segment) may be 1.05 to 1.1:1.

[0124] For example, the ratio of the minimum thickness and the average thickness of each of the at least two gas detection segments (0) (minimum thickness of each gas detection segment: average thickness of each detection segment) may be 0.91:1, 0.92:1, 0.93:1, or 0.94:1.

[0125] For example, the ratio of the maximum thickness to the average thickness of each of the at least two gas detection segments (0) (maximum thickness of each gas detection segment: average thickness of each detection segment) may be 1.06:1, 1.07:1, 1.08:1, or 1.09:1.

[0126] If the ratio of the maximum thickness to the average thickness of each of the above gas sensing segments exceeds the range of the present invention, it may be difficult to achieve uniform deposition of the gas sensing segments.

[0127] If the ratio of the maximum thickness to the average thickness of each of the above gas sensing segments falls below the range of the present invention, the difference in chemical resistance response between gas sensing segments is insufficient for accurate analysis through vector data processing, and thus the selectivity of the sensor may be significantly reduced.

[0128] In one embodiment, based on 100% of the total area of ​​the insulating substrate (1), the area of ​​each of the at least two gas sensing segments (0) may be 8% or more and 35% or less.

[0129] For example, based on 100% of the total area of ​​the insulating substrate (1), the area of ​​each of the at least two gas sensing segments (0) may be 8.5% to 30%, 9% to 25%, 9.5% to 25%, or 10% to 25%.

[0130] For example, based on 100% of the total area of ​​the insulating substrate (1), the sum of the areas of the first gas detection segment (5) to the third gas detection segment (7) may be 15% to 45%, 20% to 40%, 25% to 38%, or 28% to 35%.

[0131] If the area of ​​each of the at least two gas sensing segments (0) exceeds the range of the present invention, the difference in chemical resistance response between each gas sensing segment is not sufficient for accurate analysis through vector data processing, and the selectivity of the sensor may be significantly reduced.

[0132] In one embodiment, the upper, lower, or a combination thereof of the insulating substrate (1) may additionally include a temperature resistor (3), a heater (4), or a combination thereof.

[0133] The temperature of the gas detection sensor can be maintained at 20°C to 30°C using the above-mentioned temperature resistor (3), heater (4), or a combination thereof, and the temperature range of 20°C to 30°C may correspond to the temperature range in which the gas detection sensor operates.

[0134] Sensor power consumption can be minimized within the temperature range of the present invention, and it can be used even in flammable environments.

[0135] In one embodiment, the gas detection sensor may include a strip electrode (2) in the horizontal direction of the center of an insulating substrate (1) in the shape of a square or rectangle, and may include a pair of temperature resistors (3) and heaters (4) at each of the vertical edges of the strip electrode (2), and may include a first gas detection segment (5), a second gas detection segment (6), and a third gas detection segment (7) on the insulating substrate (1) and the strip electrode (2).

[0136] In one embodiment, the gas detection sensor may be shown in the form of FIG. 1, but is not limited thereto.

[0137] FIG. 1(a) may be a photograph of a gas detection sensor of one embodiment, and FIG. 1(b) may be a plan view of a gas detection sensor of one embodiment.

[0138] Figure 1(c) may show a cross-section cut perpendicularly along line AA of Figure 1(b).

[0139] A method for manufacturing a gas sensing sensor according to another aspect of the present invention may include the steps of: depositing the strip electrode (2) on the insulating substrate (1); applying a suspension containing the aminated graphene onto the insulating substrate (1) and the strip electrode (2); and bonding the peptide to the aminated graphene to manufacture the at least two gas sensing segments (0).

[0140] The strip electrode (2) may be made of a material including gold, platinum, or a combination thereof, and the strip electrode (2) may be deposited on the insulating substrate (1) by cathode sputtering, magnetron sputtering, ion beam deposition, thermal evaporation, or a combination thereof.

[0141] The above-mentioned suspension containing aminized graphene can be prepared by adding aminized graphene powder to alcohol.

[0142] The suspension containing the aminized graphene can be applied in the form of an aerosol onto the insulating substrate (1) and the strip electrode (2).

[0143] The above aerosol form can be applied using an airbrush, spray application, electrospray application, or a combination thereof.

[0144] In order to bind the above peptide to the aminated graphene, a solution containing the above peptide can be dropped onto the insulating substrate (1) coated with the aminated graphene and the strip electrode (2).

[0145] For example, the solution containing the peptide may be a mixture of 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC), 2-(N-morpholino)ethanesulfonic acid (MES), and the peptide, and the solution containing the peptide may be dropped onto the insulating substrate (1) and the strip electrode (2) coated with the aminated graphene using a pipette, syringe, burette, dropper, or a combination thereof, and then the peptide may be bonded to the aminated graphene through a drying process.

[0146] In one embodiment, the method for manufacturing the gas detection sensor may further include the step of forming a temperature resistor (3), a heater (4), or a combination thereof on the insulating substrate (1).

[0147] The above temperature resistor (3), heater (4), or combination thereof can form a pattern using a photomask, lithography, or a combination thereof after depositing gold, platinum, or a combination thereof on the insulating substrate (1).

[0148] A gas analysis device according to another aspect of the present invention may include the gas detection sensor.

[0149] The above gas detection sensor may be connected to a connector, and power required to drive the gas detection sensor and the heater (4) of the gas detection sensor may be provided using the connector, and the gas detection sensor may be connected to a multimeter and a PID controller (Proportional-Integral-Derivative Controller) using the connector.

[0150] The above multimeter can calculate the measurement value of the gas detection sensor and can maintain the temperature of the gas detection sensor through the PID controller.

[0151] In addition, the gas analysis device can be connected to a computer, and real-time monitoring can be performed through the computer.

[0152] For example, the gas analysis device can be connected to the computer using methods such as USB (Universal Serial Bus), Wi-Fi, Bluetooth, Ethernet, or a serial port (RS232), and through the monitoring, the presence of gas leakage, the type of leaked gas, and the concentration of the gas can be observed in real time.

[0153] A gas analysis method according to another aspect of the present invention may include: a first step of collecting each measurement value from at least two gas detection segments (0) of a gas detection sensor according to any one of claims 1 to 16 before and after gas inflow; a second step of filtering to exclude non-informative values ​​from each measurement value; a third step of vectorizing the distribution of the filtered measurement value across the entire strip electrode (2) below the at least two gas detection segments (0); a fourth step of converting the vectorized distribution of the filtered measurement value into vector data; and a fifth step of plotting a cluster representing the vector data of the gas in a reduced artificial space of features and quantifying the characteristics of the gas using Mahalanobis metrics.

[0154] The above non-informative value may be, for example, an infinite resistance value due to a malfunction of the strip electrode (2).

[0155] Meanwhile, the above-mentioned fourth step may be performed by Principal Component Analysis (PCA), Linear Discriminant Analysis (LDA), correlation analysis, artificial neural network methods, or a combination thereof, in order to distinguish and identify vectors collected from each different gas that is an analyte.

[0156]

[0157] Hereinafter, the operation and effects of the invention will be explained in more detail through specific embodiments of the invention. However, these embodiments are merely presented as examples of the invention and do not define the scope of the invention.

[0158]

[0159] [Preparation Example 1] Preparation of Aminized Graphene Powder

[0160] Aminated graphene was prepared using a liquid phase modification method, and a schematic diagram of the suspension preparation, heating, and washing process for obtaining aminated graphene is shown in Fig. 2.

[0161] A brown, homogeneous suspension was prepared by adding graphene oxide at a ratio of 5 g / ml to a solution of 75 g of urea mixed with 200 ml of formamide.

[0162] The above suspension was heated to 180°C using a Coblo heater and fed into a mechanical top-drive mixer, where it was mixed at a speed of 200 rpm for 108 hours while maintaining the temperature.

[0163] After cooling the mixture to room temperature for 20 minutes, it was washed using a Shott filter with a pore size of 16 μm. At this time, the pressure inside the filter was set to 50 Pa using a vacuum system (VACUUBRAND PC 3001 VARIO).

[0164] The generated precipitate was washed with a 3% hydrochloric acid solution until the wash water became colorless, and then washed three additional times with 25 ml of isopropyl alcohol.

[0165] The washed precipitate was dried at room temperature for 40 minutes to produce aminated graphene powder.

[0166]

[0167] [Preparation Example 2] Fabrication of a gas detection sensor

[0168] A gas detection sensor was manufactured in the following way.

[0169] A silicon oxide layer with a thickness of 370 nm was formed on a silicon insulating substrate (1) with a size of 9 x 10 mm and a thickness of 700 μm, and 39 strip electrodes (2) made of gold material with a thickness of about 100 nm and a width of 50 μm were deposited in parallel on the silicon oxide layer using a metal magnetron sputtering technique. At this time, the distance between the strip electrodes (2) was 50 μm.

[0170] Afterwards, a gold material was deposited on the edge of the insulating substrate (1) using a metal magnetron sputtering technique, and then a temperature resistor (3) and a heater (4) were formed using a lithography method.

[0171] At this time, the temperature resistor (3) was manufactured in the form of a film with a thickness of about 100 nm and a line width of 20 μm, and the heater (4) was manufactured in the form of a fine pattern (meander pattern) with a line width of 50 μm.

[0172] Afterwards, a solution containing aminized graphene was applied as an aerosol to an insulating substrate (1) on which a strip electrode (2), a temperature resistor (3), and a heater (4) were deposited, thereby forming a gas sensing segment on the strip electrode (2) and the insulating substrate (1).

[0173] A schematic diagram of the process of preparing and applying a suspension containing aminated graphene and binding a peptide to the aminated graphene is shown in Fig. 3.

[0174] The suspension containing the above-mentioned aminized graphene was prepared as follows.

[0175] A suspension was prepared by adding the aminated graphene powder prepared in Preparation Example 1 to isopropyl alcohol at a ratio of 10 μg / ml, and treated in an ultrasonic cleaner for 30 minutes at an ultrasonic frequency of 35 kHz and an oscillator output of 70 W.

[0176] The ultrasonically treated suspension was mixed using a magnetic stirrer at 200 rpm for 20 minutes.

[0177] A metal mask with a rectangular window (slit) of size 6 mm x 1.7 mm was fixed at a distance of 0.5 mm from the surface of the insulating substrate (1).

[0178] After fixing the airbrush (JAS 1142) at a distance of 150 mm from the surface of the mask, it was connected to a compressor including a drying system.

[0179] The temperature of the insulating substrate (1) was set to 170°C and raised, and a suspension containing aminized graphene was applied with dry purified air at a carrier gas pressure of 2 atm and a flow rate of 1 L / min to form a first gas sensing segment (5).

[0180] Then, the mask was moved 1.7 mm perpendicular to the parallel electrode to form the second gas detection segment (6) in the same way as the first gas detection segment (5) was formed.

[0181] Then, the mask was moved 1.7 mm in the same direction (perpendicular to the parallel electrode) to form the third gas detection segment (7) in the same way.

[0182] At this time, the suspension consumed to manufacture the first gas detection segment (5), the second gas detection segment (6), and the third gas detection segment (7) was 7.7 ml, 15.3 ml, and 11.6 ml, respectively.

[0183] Afterwards, the insulating substrate (1) having formed the gas sensing segment was naturally dried at room temperature for 2 hours, then heated at 80°C for 60 minutes to completely remove the residual solvent to produce an insulating substrate (1) with deposited aminated graphene, and then a peptide was bonded to the aminated graphene.

[0184] After fixing the rectangular mask again on the surface of the first gas sensing segment (5), the first peptide was combined.

[0185] The mixture used for the first peptide bond was prepared by mixing 1.0 mg of 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC) and 90 μl of 2-(N-morpholino)ethanesulfonic acid (MES) at a speed of 180 rpm until dissolved, then adding the first peptide at a concentration of 0.1 μg / μl and homogenizing by 20 pipettings.

[0186] After applying 75 μl of the mixture used for the first peptide bond to the surface of the first gas sensing segment (5), the reaction was carried out at room temperature for 2 hours. After the reaction, the excess solution was removed with a pipette, washed with a mixture of Tween 20 and PBS (Phosphate Buffered Saline), and then dried in the air.

[0187] Each mixture prepared by mixing the second peptide and the third peptide in the same way was combined with the second peptide and the third peptide in the same way as the first peptide was combined with the aminated graphene containing the second gas sensing segment (6) and the third gas sensing segment (7).

[0188] The above first to third peptides are shown in Table 1 below.

[0189] Sequence 1 Peptide Cysteine-Arginine-Glutamine-Valine-Phenylalanine (CRQVF) 2 Peptide Cysteine-Isoleucine-Histidine-Asparagine-Proline (CIHNP) 3 Peptide Tryptophan-Histidine-Valine-Serine-Cysteine ​​(WHVSC)

[0190] A photograph, a top view, and a left side view of the gas detection sensor manufactured in this way are shown in FIGS. 1(a) to 1(c), respectively.

[0191] In FIG. 1(b) and FIG. 1(c), 1 to 7 respectively represent an insulating substrate, a strip electrode, a temperature resistor, a heater, a first gas sensing segment, a second gas sensing segment, and a third gas sensing segment.

[0192]

[0193] [Example 1] Preparation of a gas analyzer

[0194] The gas detection sensor manufactured according to Manufacturing Example 2 was placed inside a stainless steel chamber (48) and its position was fixed using a 50-pin ceramic holder, and an intake pipe for gas intake and an exhaust pipe for gas exhaust were formed in the chamber.

[0195] The above 50-pin ceramic holder is rectangular in shape and can transmit current to the strip electrode (2) through gold-plated contact pads, and is manufactured so that an Erni SMC connector (pin spacing 1.27 mm) is connected to the bottom.

[0196] The Erni SMC connector of the gas detection sensor was assembled using ultrasonic welding (WEST Bond 747677E, USA) with a micro wire (diameter 38 μm) so that it could be exposed to the outside of the chamber, and then the chamber was sealed.

[0197] Power required to drive the gas detection sensor and the heater (4) of the gas detection sensor was provided using the above connector, and a gas analysis device was manufactured by connecting a multimeter and a PID controller (Proportional-Integral-Derivative Controller) for measuring the measurement value.

[0198]

[0199] [Evaluation Example 1] Verification of the thickness of the gas detection segment

[0200] The thickness of the gas sensing segment prepared according to Preparation Example 2 was confirmed using a scanning electron microscope (Scanning Electron Microscope, JEOL JSM-7001F).

[0201] Photos taken of the sides of the first gas detection segment (5) to the third gas detection segment (7) using a scanning electron microscope are shown in FIGS. 4(a) to 4(c), and it was confirmed that the average thickness of the first gas detection segment (5) to the third gas detection segment (7) was 41 nm, 12 nm, and 28 nm, respectively.

[0202]

[0203] [Evaluation Example 2] Verification of the surface area of ​​the gas detection segment

[0204] As a result of confirming the surface areas of the first gas detection segment (5) to the third gas detection segment (7) through specific surface area analysis (Brunauer-Emmett-Teller, BET), the average specific surface area of ​​the first gas detection segment (5) to the third gas detection segment (7) is 300 m 2 It was confirmed as / g.

[0205] The average specific surface area of ​​the first gas detection segment (5) to the third gas detection segment (7) is 50 m 2 / g to 500 m 2 It can be / g.

[0206] For example, 100 m 2 / g to 450 m 2 / g, 150 m 2 / g to 400 m 2 / g or 200 m 2 / g to 350 m 2 It can be / g.

[0207]

[0208] [Evaluation Example 3] Confirmation of the number of aminized graphene atoms and the binding ratio of peptides

[0209] The ratio of nitrogen atoms in aminated graphene was determined using X-ray Photoelectron Spectroscopy (XPS, Thermo Fisher ESCALAB 250Xi XPS system).

[0210] First, using the integral values ​​of the carbon (C) 1s (24), oxygen (O) 1s (25), and nitrogen (N) 1s (26) spectrum lines of the XPS graph of the amine graphene shown in Fig. 5, the number of nitrogen atoms among the total atoms of graphene and amine was calculated and found to be 13.21 atomic%.

[0211] Among these, the ratio of polymeric amines was checked to determine the atomic percentage of amines bonded to graphene.

[0212] High-resolution decomposition analysis of the nitrogen 1s and carbon 1s lines of the XPS graph above is shown in FIG. 6(a) and FIG. 6(b), and as a result of the decomposition analysis of the amine (28) and CN (33) spectrum lines, the proportion of the polymer amine group was confirmed to be 69.6%.

[0213] Through this, it was confirmed that the number of amine atoms bonded to graphene was 9.2 atomic percent, based on 100% of the total atoms of graphene and amine.

[0214] In addition, graphs of the first gas detection segment (5) to the third gas detection segment (7) measured using X-ray photoelectron spectroscopy are shown in FIGS. 7(a) to 7(c). In the XPS measurement graph of the first gas detection segment (5) to the third gas detection segment (7), sulfur (S) 2p (27) spectrum lines could be identified, and through this, it was found that peptides were bound to the amine graphene.

[0215] It was confirmed that the ratio of peptides bound to the amine graphene among the first to third peptides introduced was 47%, 44%, and 51%, respectively.

[0216]

[0217] [Evaluation Example 4] Verification of resistance change in gas sensing segment

[0218] Changes in resistance values ​​according to exposure time to methanol (CH3OH), ethanol (C2H5OH), acetone (CH3COCH3), ammonia (NH3), and water vapor (H2O) of the first gas detection segment (5) to the third gas detection segment (7) of the gas detection sensor prepared according to Preparation Example 2 were measured and are shown in FIGS. 8(a) to FIGS. 12(c), respectively.

[0219] Through this, it was confirmed that the resistance value of each segment increased when exposed to gas, and that the resistance value increased significantly as the exposure time increased.

[0220]

[0221] [Evaluation Example 5] Verification of current ratio to voltage of gas detection segment

[0222] The current according to the voltage was measured when the first gas detection segment (5) to the third gas detection segment (7) of the gas detection sensor manufactured according to Manufacturing Example 2 was exposed to dry air at 25°C, and this is shown in FIG. 13.

[0223] As a result of the measurement, it was confirmed that the ratio of current to voltage of each gas sensing segment was in the form of a straight line with a constant slope, which may mean that there is no potential barrier in the contact between the gas sensing segment and the strip electrode (2).

[0224]

[0225] [Evaluation Example 6] Verification of chemical resistance response according to gas concentration

[0226] A device capable of introducing a mixed gas was additionally connected to the gas analysis device of Example 1 to measure the chemical resistance response according to the gas concentration, and a schematic diagram of the device capable of introducing a mixed gas to the gas analysis device is shown in FIG. 14.

[0227] Specifically, the air compressed by the air compressor (37) is dried through a dryer (adyabatic type, 38), and then divided by a pressure regulator (39) into air going to a multiplexer (44) and air going to a flow meter (40), and connected using an Ethernet protocol.

[0228] A portion of the air directed toward the flow meter was mixed again with air dried by passing through a bubbler (41) with distilled water, and the air mixed again was mixed with the air directed toward the multiplexer and analyzed by a gas analysis device.

[0229] The air transferred to the multiplexer is sequentially mixed with ammonia, ethanol, acetone, methanol, and water vapor by the bubbler (43), passes through the electromagnetic valve (47), and is then mixed with the air that was directed to the flow meter and analyzed by the gas analysis device.

[0230] Meanwhile, the shaft (46) having a support disc for tube compression is a device for precisely controlling the supply of analytes from the bubbler.

[0231] Chemical resistance responses according to gas concentrations of the first gas sensing segment (5) to the third gas sensing segment (7) were measured for methanol (CH3OH), ethanol (C2H5OH), acetone (CH3COCH3), ammonia (NH3), and water vapor (H2O).

[0232] The chemical resistance response was measured in the range of each gas concentration from 0.5 to 10 kppm.

[0233] As a result of the measurement, it was confirmed that the chemical resistance response (S) changes with gas concentration (C) as an exponential function (or power law) of the form of Equation 1 below, and the range of α values ​​below was measured and shown in Table 2 below.

[0234]

[0235] <Equation 1>

[0236] Chemical resistance response (S) = Gas concentration (C) α

[0237]

[0238] α Value 1st Gas Detecting Segment 2nd Gas Detecting Segment 3rd Gas Detecting Segment Methanol 0.442±0.026 0.467±0.025 0.484±0.021 Ethanol 0.440±0.033 0.568±0.017 0.592±0.043 Acetone 0.470±0.497 0.524±0.075 0.505±0.031 Ammonia 0.489±0.020 0.463±0.021 0.517±0.030 Water Vapor 0.543±0.027 0.537±0.022 0.518±0.029

[0239] Graphs of the chemical resistance response according to the respective gas concentrations for methanol (CH3OH), ethanol (C2H5OH), acetone (CH3COCH3), ammonia (NH3), and water vapor (H2O) within the range of α values ​​for each gas are shown in the form of linear functions in FIGS. 15 to 19.

[0240] According to Evaluation Example 6, it was confirmed that the chemical resistance response appears in the form of an exponential function depending on the gas concentration, and that the first gas sensing segment (5) to the third gas sensing segment (7) have different ranges of α values ​​for each gas.

[0241]

[0242] [Evaluation Example 7] Confirmation of Gas Type in Mixed Gas

[0243] A gas mixture of air, methanol (CH3OH), ethanol (C2H5OH), acetone (CH3COCH3), ammonia (NH3), and water vapor (H2O) at a concentration of 4,000 ppm was introduced into the gas analysis device of Example 1.

[0244] After measuring the chemical resistance response of the first gas detection segment (5) to the third gas detection segment (7) for the mixed gas, the measured values ​​were pre-processed with a logarithm and then converted into vector signals.

[0245] The signaled vector was processed using pattern recognition technology through Linear Discriminant Analysis (LDA), and various analysis items in the recorded vector data were selectively distinguished to create a 3D image, which is shown in Fig. 20.

[0246] Specifically, raw multisensor signals exposed to each gas were represented as classes, and the maximum ratios between and within-class variations were estimated and transmitted to a reduced artificial space of features (LDA space).

[0247] The dimension of this LDA space is equal to (N-1), where N is the number of classes (i.e., the number of each gas being measured).

[0248] In the experiment, the following multi-sensor signals were processed.

[0249] Data recorded for air, methanol, ethanol, ammonia, acetone, and humidity (N=6);

[0250] Accordingly, a 5-dimensional LDA space was constructed based on the processed data.

[0251] Given the generated LDA space, the gravity center of each class was determined, and the uniqueness of multi-sensor data associated with each gas was quantified by further distancing using the Mahalanobis matrix.

[0252] In other words, it was confirmed that the types of mixed gases can be distinguished through vector signaling and linear discriminant analysis.

[0253] The foregoing description of the present invention is for illustrative purposes only, and those skilled in the art will understand that other specific forms can be easily modified without altering the technical spirit or essential features of the present invention. Therefore, the embodiments described above should be understood as illustrative in all respects and not restrictive.

[0254] The scope of the present invention is defined by the claims set forth below rather than by the detailed description above, and all modifications or variations derived from the meaning and scope of the claims and equivalent concepts thereof should be interpreted as being included within the scope of the present invention.

[0255]

[0256] [Explanation of the symbol]

[0257] 0: 2 or more gas detection segments

[0258] 1: Insulating substrate

[0259] 2: Strip electrode

[0260] 3: Thermoresistor

[0261] 4: Heater

[0262] 5: 1st gas detection segment

[0263] 6: Second gas detection segment

[0264] 7: Third gas detection segment

[0265] 8: Step to prepare the suspension (mixture of formamide, urea, and graphene oxide)

[0266] 9: Heating step of the suspension

[0267] 10: Suspension washing step using a shot filter

[0268] 11: Step for preparing a suspension with added aminized graphene powder

[0269] 12: Ultrasonic cleaning step

[0270] 13: Mixing step using a magnetic stirrer

[0271] 14: Gas sensing segment formation (deposition) step

[0272] 15: Room temperature natural drying step

[0273] 16: Heating step

[0274] 17: Mask fixation step

[0275] 18: Step for preparing a mixture of 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC) and 2-(N-morpholino)ethanesulfonic acid (MES)

[0276] 19: Peptide addition to the mixture and homogenization step

[0277] 20: Step of applying the mixture used for peptide bonding

[0278] 21: Excess solution removal step

[0279] 22: Cleaning step

[0280] 23: Drying stage

[0281] 24: C 1s peak

[0282] 25: O 1s peak

[0283] 26: N 1s peak

[0284] 27: S 2p peak

[0285] 28: Primary amine peak

[0286] 29: Pyridine nitrogen peak

[0287] 30: Substituted nitrogen peak

[0288] 31: Pyridine N-oxide peak

[0289] 32: C=C bonding peak

[0290] 33: CN binding peak

[0291] 34: C-OH bonding peak

[0292] 35: C=O bonding peak

[0293] 36: COOH coupling peak

[0294] 37: Air Compressor

[0295] 38: Dryer (Adiabatic type)

[0296] 39: Pressure regulator reducer

[0297] 40: Flow meter

[0298] 41: Bubbler

[0299] 42: Needle valve

[0300] 43: Bubbler

[0301] 44: Multiplexer

[0302] 45: Electric motor

[0303] 46: Shaft with support disc for tube crimping

[0304] 47: Electromagnetic valve (3-way)

[0305] 48: Chamber

[0306] 49: Electromagnetic shielding box

[0307] 50: Power supply

[0308] 51: Commercial Humidity and Gas Analyzer

[0309] 52: Multimeter

[0310] 53: PID controller

[0311] 54: Power supply (for heater)

[0312] 55: Personal computer

[0313]

[0314] According to the gas detection sensor, gas analysis device, and gas analysis method of the present invention, it is possible to analyze what type of gas is mixed in a mixed gas with excellent sensitivity and selectivity, and the gas analysis device can be operated stably at room temperature.

Claims

1. Insulating substrate (1); Strip electrode (2); and It includes at least 2 gas detection segments (0), and The strip electrode (2) is formed on the insulating substrate (1), and The above at least two gas sensing segments (0) are formed on the insulating substrate (1) and the strip electrode (2). Gas detection sensor.

2. In Paragraph 1, The insulating substrate (1) comprises quartz, glass, ceramic, silicon oxide, or a combination thereof. Gas detection sensor.

3. In Paragraph 1, The width of the strip electrode (2) is 1 μm to 100 μm, Gas detection sensor.

4. In Paragraph 1, The thickness of the strip electrode (2) is 50 nm to 1,000 nm, Gas detection sensor.

5. In Paragraph 1, The number of the strip electrodes (2) is 2 or more, and The distance between the strip electrodes (2) is 1 μm to 100 μm, Gas detection sensor.

6. In Paragraph 1, The above at least two gas sensing segments (0) comprise aminized graphene, and Based on a total atomic percentage of 100 atomic percentages of the above-mentioned aminized graphene, the atomic percentage of the amine is 7 atomic percentages or more and 20 atomic percentages or less, Gas detection sensor.

7. In Paragraph 6, The above-mentioned amine graphene combines with a peptide, Gas detection sensor.

8. In Paragraph 7, The above peptides are Cysteine-Isoleucine-Histidine-Asparagine-Proline (CIHNP), Cysteine-Arginine-Glutamine-Valine-Phenylalanine (CRQVF), Tryptophan-Histidine-Valine-Serine-Cysteine ​​(WHVSC), and Aspartic acid-Serine-Tryptophan-Alanine-Alanine-Aspartic acid-Isoleucine-Proline (DSWAADIP). Aspartic acid-Asparagine-Proline-Isoleucine-Glutamine-Alanine-Valine-Proline (DNPIQAVP), Tryptophan-Histidine-Tyrosine-Glutamine-Arginine-Proline-Leucine-Methionine-Proline-Valine-Serine-Isoleucine (WHYQRPLMPVSI), Lysine-Serine-Aspartic acid-Serine-Cysteine ​​(KSDSC), Leucine-Glycine-Phenylalanine-Aspartic Acid-Cysteine ​​(LGFDC),Asparagine-Glutamine-Leucine-Serine-Asparagine-Leucine-Serine-Phenylalanine-Serine-Aspartic acid-Leucine-Cysteine ​​(NQLSNLSFSDLC), Valine-Phenylalanine-Serine-Isoleucine-Leucine-Serine-Proline-Leucine-Proline-Leucine-Isoleucine-Isoleucine-Proline-Phenylalanine-Valine-Cysteine ​​(Valine-Phenylalanine-Serine-Isoleucine-Leucine-Serine-Proline-Leucine-Proline-Leucine-Proline-Isoleucine-Isoleucine-Proline-Phenylalanine-Valine-Cysteine, VFSILSPLPLIIPFVC), cysteine-isoleucine-glutamine-proline-valine (CIQPV), or a combination thereof, comprising Gas detection sensor.

9. In Paragraph 7, The peptides included in each of the gas sensing segments of the at least two gas sensing segments (0) are different from each other. Gas detection sensor.

10. In Paragraph 8, The above at least two gas detection segments (0) include a first gas detection segment (5), a second gas detection segment (6), and a third gas detection segment (7), and The peptide of the first gas sensing segment (5) comprises cysteine-arginine-glutamine-valine-phenylalanine (CRQVF), and The peptide of the second gas sensing segment (6) comprises cysteine-isoleucine-histidine-asparagine-proline (CIHNP), and The peptide of the third gas sensing segment (7) comprises tryptophan-histidine-valine-serine-cysteine ​​(WHVSC). Gas detection sensor.

11. In Paragraph 1, Detecting methanol (CH3OH), ethanol (C2H5OH), acetone (CH3COCH3), ammonia (NH3), water vapor (H2O), acetonitrile, ethyl acetate, nonanal, octanal, terpin-4-ol, ethylbutanoate, ethyl octanoate, hexane, trinitrotoluene, or combinations thereof, Gas detection sensor.

12. In Paragraph 1, The thickness of at least two gas sensing segments (0) is 5 nm to 100 nm, Gas detection sensor.

13. In Paragraph 1, The average thickness of each of the at least two gas detection segments (0) is the same or different. Gas detection sensor.

14. In Paragraph 1, The ratio of the minimum thickness to the average thickness of each of the at least two gas detection segments (0) (minimum thickness of each gas detection segment: average thickness of each detection segment) is 0.9 to 0.95:1, and The ratio of the maximum thickness to the average thickness of each of the at least two gas detection segments (0) (maximum thickness of each gas detection segment: average thickness of each detection segment) is 1.05 to 1.1:1, Gas detection sensor.

15. In Paragraph 1, Based on 100% of the total area of ​​the insulating substrate (1), the area of ​​each of the at least two gas sensing segments (0) is 8% or more and 35% or less, Gas detection sensor.

16. In Paragraph 1, A temperature resistor (3), a heater (4), or a combination thereof additionally comprising the upper, lower, or a combination thereof on the insulating substrate (1), Gas detection sensor.

17. A method for manufacturing a gas detection sensor according to any one of claims 1 to 16, A step of depositing the strip electrode (2) on the insulating substrate (1); A step of applying a suspension containing the aminized graphene onto the insulating substrate (1) and the strip electrode (2); and The method comprises the step of manufacturing at least two gas sensing segments (0) by binding the above peptide to the above aminated graphene. Method for manufacturing a gas detection sensor.

18. In Paragraph 17, The method further comprises the step of forming a temperature resistor (3), a heater (4), or a combination thereof on the insulating substrate (1). Method for manufacturing a gas detection sensor.

19. A gas detection sensor comprising any one of paragraphs 1 to 16, Gas analysis device.

20. A first step of collecting respective measurement values ​​from at least two gas detection segments (0) of a gas detection sensor according to any one of claims 1 to 16 before and after gas inflow; A second step of filtering to exclude non-informative values ​​from each of the above measurements; A third step of vectorizing the distribution of the filtered measurements across the entire strip electrode (2) below at least two gas detection segments (0); A fourth step of converting the distribution of the filtered measurements vectorized above into vector data; and A fifth step of plotting clusters representing the vector data of the gas in a reduced artificial space of features and quantifying the characteristics of the gas using Mahalanobis metrics; The above-mentioned fourth step is performed by Principal Component Analysis (PCA), Linear Discriminant Analysis (LDA), correlation analysis, artificial neural network methods, or a combination thereof, Gas analysis method.

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