Method and kit for extracting DNA from complex samples
A PEG-based DNA extraction method for plant samples addresses speed, contamination, and cost issues by replacing alcoholic and chaotropic buffers, ensuring efficient and cost-effective high-throughput DNA extraction.
WO2026146177A1PCT designated stage Publication Date: 2026-07-09IST INNUSCREEN GMBH
Patent Information
- Authority / Receiving Office
- WO · WO
- Patent Type
- Applications
- Current Assignee / Owner
- IST INNUSCREEN GMBH
- Filing Date
- 2025-12-30
- Publication Date
- 2026-07-09
Smart Images
Figure EP2025089181_09072026_PF_FP_ABST
Abstract
The invention relates to a method and a kit for extracting DNA from biological samples after optionally homogenizing and lysing the samples, the method having the steps of: a) binding the DNA to a solid phase by means of a binding buffer, b) washing, multiple times, the DNA bound to the particles using a washing buffer, c) drying the particles, and d) the eluting the DNA, wherein neither the binding buffer nor the washing buffer comprises combustible aliphatic alcoholic components or any chaotropic salts, the invention being characterized in that both the binding buffer and the washing buffer have a combination of water-soluble polymers, preferably PEG, and water-soluble salts as active components.
Need to check novelty before this filing date? Find Prior Art