Combination of culture medium components

Abstract

The present disclosure provides a culture medium for cell culturing, the culture medium containing a combination of specific components. The present disclosure provides a culture medium which contains a combination of components selected from the group consisting of: (1) a halcinonide component; (2) an ALK4 / 7 inhibitor or a TGFβ receptor type-1 kinase inhibitor; (3) a BRD7 / 9 double degradation factor or a BRD7 / 9 binding molecule (degradation factor); (4) an LATS1 / 2 inhibitor, a PKA inhibitor, or an AKT1 inhibitor; (5) a CK1 / 2 inhibitor, an insulin receptor inhibitor, a Tyr kinase inhibitor, a PKA inhibitor or a PKC inhibitor, or an adenosine kinase inhibitor; (6) an E-selectin inhibitor, a VCAM1 inhibitor, or an ICAM1 inhibitor; and (7) an LATS1 inhibitor + an LATS2 inhibitor.

Description

Combinations of culture medium components

[0001] This disclosure relates to a culture medium for cells containing specific components (particularly various stem cells such as mesenchymal stem cells (MSCs)). This disclosure particularly concerns the composition and use of a culture medium suitable for the proliferation and maintenance of various stem cells, such as mesenchymal stem cells.

[0002] Cell culture typically requires a culture medium containing specific components. These components are essential for promoting cell proliferation, maintenance, and the expression of specific functions. However, conventional culture media may lack certain components necessary for optimizing cell proliferation. This disclosure provides an improved culture medium to address these issues.

[0003] In recent years, research into the development of pharmaceuticals and regenerative medicine utilizing living cells or tissues has been progressing and attracting attention. In particular, research into organ regeneration technologies using pluripotent stem cells such as ES cells and iPS cells is accelerating. On the other hand, cell therapy using somatic stem cells such as bone marrow stem cells utilizes the inherent function of somatic stem cells to repair tissue damaged by disease, and is attracting attention as a more feasible form of regenerative medicine, with research progressing accordingly.

[0004] Various stem cells, such as mesenchymal stem cells (hereinafter also referred to as MSCs), are pluripotent somatic stem cells that can usually be isolated from bone marrow, adipose tissue, umbilical cord, or peripheral blood, and have the ability to differentiate into many different types of cells.

[0005] As a result of various studies conducted by the inventors, it was discovered that high-quality mesenchymal stem cells can be obtained by treating cells, particularly mesenchymal stem cells, with at least one specific component, thus completing the present invention.

[0006] In other words, this disclosure relates to at least the following:

[0007] In this section, (1) to (7) are as follows: (1) Hallucinonides and the following hallucinonides (Here, R １ and R ２is independently selected from the group consisting of hydrogen, alkyl, alkoxy, hydroxy, and OR (where R is hydrogen or alkyl), or R １ and R ２ together form a ring, and R ３ is halo or hydrogen, and R ４ is halo (preferably chloro) or optionally substituted hydroxy), or a pharmaceutically acceptable salt or solvate thereof (2) ALK4 / 7 inhibitor or TGFβ receptor type 1 kinase inhibitor (3) BRD7 / 9 bifunctional degrader or BRD7 / 9 binding molecule (degrader) (4) LATS1 / 2 inhibitor, PKA inhibitor or AKT1 inhibitor (5) CK1 / 2 inhibitor, insulin receptor Tyr kinase inhibitor, PKA inhibitor or PKC inhibitor or adenosine kinase inhibitor (6) E-selectin inhibitor, VCAM1 inhibitor or ICAM1 inhibitor; and (7) Lats1 inhibitor + Lats2 inhibitor (either one can be inhibited)

[0008] (1') to (7') are as follows: (1') In (1), R １ and R ２ are independently selected from the group consisting of hydrogen, alkyl, alkoxy, hydroxy, and OR (where R is hydrogen or alkyl) (2') TGFβ receptor type 1 kinase inhibitor (3') BRD7 / 9 binding molecule (degrader) (4') AKT1 inhibitor (5') Adenosine kinase inhibitor (6') ICAM1 inhibitor; and (7') Lats1 inhibitor

[0009] (1") to (7") are as follows: (1") In (1), R １ and R ２These combine to form a ring: (2) ALK4 / 7 inhibitor (3) BRD7 / 9 dual degradation factor (4) LATS1 / 2 inhibitor or PKA inhibitor (5) CK1 / 2 inhibitor, insulin receptor Tyr kinase inhibitor, PKA inhibitor or PKC inhibitor; (6) E-selectin inhibitor or VCAM1 inhibitor; and (7) Lats2 inhibitor

[0010] (1A) to (7A) are as follows: (1A) or or a pharmaceutically acceptable salt or solvate thereof; (2A) TGFβ receptor type 1 kinase inhibitor which is also an ALK4 / 7 inhibitor; (3A) BRD7 / 9 binding molecule (degradation factor) which is also a BRD7 / 9 dual degradation factor; (4A) AKT1 inhibitor which is also a LATS1 / 2 inhibitor or a PKA inhibitor; (5A) Adenosine kinase inhibitor which is also a CK1 / 2 inhibitor, insulin receptor Tyr kinase inhibitor, PKA inhibitor or PKC inhibitor; (6A) ICAM1 inhibitor which is also an E-selectin inhibitor or a VCAM1 inhibitor; and (7A) Lats1 / 2 inhibitor (that inhibits both).

[0011] (1B) to (7B) are as follows: (1B) Hallucinonide or a pharmaceutically acceptable salt or solvate thereof (2B) AZ12601011 or a pharmaceutically acceptable salt or solvate thereof (3B) VZ185 or a pharmaceutically acceptable salt or solvate thereof (4B) A-674563 or a pharmaceutically acceptable salt or solvate thereof (5B) 5-iodotubercidin or a pharmaceutically acceptable salt or solvate thereof (6B) ICAM-1-IN-1 or a pharmaceutically acceptable salt or solvate thereof; and (7B) LATS-IN-1 or a pharmaceutically acceptable salt or solvate thereof

[0012] Examples including Form 1-2 (1) and (2): [Item 1-2-1] A culture medium including (1) and (2). [Item 1-2-2] A culture medium according to any of the above items, including (1') and (2'). [Item 1-2-3] A culture medium according to any one of the above items, including (1") and (2"). [Item 1-2-4] A culture medium according to any one of the above items, including (1A) and (2A). [Item 1-2-5] A culture medium according to any one of the above items, including (1B) and (2B). [Item 1-2-5A] A culture medium according to any one of the above items, including one or more of (1), (1'), (1"), (1A) and (1B), and one or more of (2), (2'), (2"), (2A) and (2B). [Item 1-2-6] A culture medium according to any one of the above items, for culturing various stem cells such as mesenchymal stem cells. [Item 1-2-6A] A culture medium according to any one of the above items, for culturing mesenchymal stem cells. [Item 1-2-6B] A serum-free culture medium according to any one of the above items. [Item 1-2-6C] A culture medium according to any one of the above items, which does not contain heterologous raw materials. [Item 1-2-7] A method for producing cells, comprising the step of culturing cells using the culture medium according to any one of the above items. [Item 1-2-8] The method according to any one of the above items, wherein the culturing step comprises culturing under conditions that satisfy at least one selected from the group consisting of a specific range of cell numbers, a specific container, and a specific temperature. [Item 1-2-8A] The range of cell numbers is such that MSCs are not seeded at low densities, preferably 500 cells / cm². ２ ~20000 cells / cm ２ That's good enough, and more preferably, 500-1000 / cm ２ ~10,000 / cm ２ For example, 2000 cells / cm² ２The method according to any one of the above items, although often seeded before or after. [Item 1-2-8B] The method according to any one of the above items, wherein the range of the cell number is a specific value such as 1500 cells / well during screening, and / or is carried out under conditions such as 4500 cells / well which are estimated to become confluent. [Item 1-2-8C] The method according to any one of the above items, wherein the specific container is a container of a suitable material (including with or without coating). [Item 1-2-8D] The method according to any one of the above items, wherein the specific temperature is a suitable temperature (such as 25-37°C or 30°C). [Item 1-2-9] A cell culture apparatus comprising a culture medium according to any one of the above items or means for providing the culture medium, a container capable of containing the culture medium, and means for providing conditions for culturing cells as necessary. [Item 1-2-10] A method for preserving cells, comprising the step of preserving cells using the culture medium according to any one of the above items. [Item 1-2-11] A composition for preserving cells, comprising the component described in any one of the above items. [Item 1-2-12] A cell-containing composition comprising the component described in any one of the above items and cells. [Item 1-2-13] Cells cultured using the component described in any one of the above items. [Item 1-2-13A] The cells described in any one of the above items, wherein the expression of at least one selected from the group consisting of CD44, CD73, CD90, and CD105 is not significantly reduced. [Item 1-2-13B] A cell population comprising the cells described in any of the above items, wherein the expression of at least one selected from the group consisting of CD44, CD73, CD90, and CD105 is not significantly reduced even after long-term passage. [Item 1-2-13C] The marker is at least one of CD44, CD73, CD90, and CD105, and at least one of these does not decrease. Typically, CD44 does not decrease, but the others often decrease, and it is a desirable and advantageous characteristic that CD73 and CD90, or CD44, CD90 and CD105, do not decrease, in any one of the above items, a cell or cell population.[Item 1-2-13D] Cells or cell populations according to any one of the above items, in which high expression of CD90 is maintained. [Item 1-2-13E] Cells or cell populations according to any one of the above items, in which the expression of at least one of the markers (preferably at least one of CD73, CD90, and CD105) is not significantly reduced when the number of passages is 5 or more, preferably 6 or more, and more preferably 7 or more. [Item 1-2-13F] Cells or cell populations according to any one of the above items, in which the expression of at least one of the markers (preferably at least one of CD73, CD90, and CD105) is not significantly reduced when the number of passages is 8 or more. [Item 1-2-13G] Cells or cell populations according to any one of the above items, in which differentiation ability is maintained when the number of passages is 5 or more, preferably 6 or more, and more preferably 7 or more. [Item 1-2-13H] Cells or cell populations according to any one of the above items, in which differentiation ability is maintained when the number of passages is 8 or more. [Item 1-2-13I] A cell or cell population according to any one of the above items, wherein the expression of CD90, CD44, and CD73 is maintained at 70% or more, usually 80% or more, preferably 90% or more, and more preferably 95% or more, respectively, before passage. [Item 1-2-13J] A cell or cell population according to any one of the above items, wherein the expression of CD105 is maintained at 30% or more, usually 40% or more, preferably 50% or more, and more preferably 70% or more, before passage. [Item 1-2-13AA] The cell according to Item 1-2-13, wherein the cell properties are improved (for example, cartilage differentiation ability is enhanced). [Item 1-2-13AB] A cell population containing about 50% or more, about 60% or more, about 70% or more, about 80% or more, about 90% or more, or more, of the cells according to any one of Items 1-2-13 to 1-13AA. [Item 1-2-14] A pharmaceutical product comprising cells described in any one of the above items. [Item 1-2-15] A method for differentiating cells, comprising the step of culturing cells obtained using a culture medium described in any one of the above items under conditions that induce differentiation. [Item 1-2-15A] The method according to any one of the above items, wherein the culture comprises culturing the cells after proliferation while observing a different differentiation medium.[Item 1-2-15B] The method according to any one of the above items, wherein the conditions under which the differentiation is induced also include differentiation conditions that are commonly used for MSCs. [Item 1-2-15C] The method according to any one of the above items, wherein the cells are less likely to become cartilage and / or have high cartilage differentiation potential. [Item 1-2-16] Differentiated cells obtained by differentiating stem cells by the method according to any one of the above items. [Item 1-2-17] A method for treating or preventing a subject, comprising the step of administering an effective amount of the cells or the pharmaceutical described in any one of the above items to a subject in need thereof. [Item 1-2-18] A method for quality control of stem cells, comprising determining whether they have a coefficient C correlated with the culture medium described in any one of the above items. [Item 1-2-18A] The method according to any one of the above items, wherein the coefficient C, in the case of cells, includes a coefficient relating to cell markers. [Item 1-2-18B] The method according to any one of the above items, wherein the cell marker includes common markers, such as cell markers related to proliferation. [Item 1-2-18C] The method according to any of the above items, wherein the cell marker includes markers described herein, for example, those that enhance differentiation ability or those that are expressed over a long period of time. [Item 1-2-18D] The method according to any of the above items, wherein the marker, in the case of a culture medium, includes information relating to the identification of the component itself. [Item 1-2-19] A system for culturing cells, comprising: 1) a cell culture section for containing cells intended for culture; 2) a medium supply section for providing a culture medium or raw materials according to any one of the above items; and 3) a cell culture condition adjustment section for adjusting culture conditions as necessary. [Item 1-2-20] A culture medium suitable for transporting cells containing culture medium components according to any one of the above items. [Item 1-2-21] A method for managing cell culture for cells suitable for culturing using a culture medium according to any one of the above items, comprising: 1) providing information relating to the culture medium for cells provided by the user; 2) assigning identifiable labels to the cells and the culture medium; and 3) managing whether the cells and the culture medium are compatible by referring to the labels.

[0013] [Item 1-2-22] The use of (1) and (2) described in Item 1-2-1 for culturing cells (preferably mesenchymal stem cells). [Item 1-2-23] The use of Item 1-2-22 for culturing cells (preferably mesenchymal stem cells), comprising the features of any one of Items 1-2-2 to 1-2-6C. [Item 1-2-24] A method for maintaining or improving the quality of cells (preferably mesenchymal stem cells) (preferably, the expression level of at least one selected from the group consisting of CD44, CD73, CD90 and CD105, and / or differentiation potential after long-term passage), comprising the step of contacting the cells in a culture medium containing the components described in Item 1-2-1. [Item 1-2-25] The method of Item 1-2-24, comprising the features of any one of Items 1-2-2 to 1-2-6C. [Item 1-2-26] Cells or cell populations described in any one of items 1-2-13 to 1-2-13AB, or differentiated cells or cell populations described in item 1-2-16, for use in treatment. [Item 1-2-27] Cells or cell populations described in any one of items 1-2-13 to 1-2-13AB, or differentiated cells or cell populations described in item 1-2-16, for use in regenerative medicine or in the treatment of cartilage diseases. [Item 1-2-28] Use of cells or cell populations described in any one of items 1-2-13 to 1-2-13AB, or differentiated cells or cell populations described in item 1-2-16, in the manufacture of drugs for regenerative medicine or in the treatment of cartilage diseases. [Item 1-2-29] A method for performing regenerative medicine or treating cartilage disease in a subject in need thereof, comprising the step of administering to the subject an effective amount of cells or cell populations described in any one of items 1-2-13 to 1-2-13AB, or differentiated cells or cell populations described in item 1-2-16.

[0014] Form 1-3 [Item 1-3-1] A culture medium comprising (1) and (3). [Item 1-3-2] A culture medium according to any of the above items, comprising (1') and (3'). [Item 1-3-3] A culture medium according to any one of the above items, comprising (1") and (3"). [Item 1-3-4] A culture medium according to any one of the above items, comprising (1A) and (3A). [Item 1-3-5] A culture medium according to any one of the above items, comprising (1B) and (3B). [Item 1-3-5A] A culture medium according to any one of the above items, comprising one or more of (1), (1'), (1"), (1A) and (1B), and one or more of (3), (3'), (3"), (3A) and (3B). [Item 1-3-6] A culture medium according to any one of the above items, for culturing various stem cells such as mesenchymal stem cells. [Item 1-3-6A] A culture medium according to any one of the above items, for culturing mesenchymal stem cells. [Item 1-3-6B] A serum-free culture medium according to any one of the above items. [Item 1-3-6C] A culture medium according to any one of the above items, which does not contain heterologous raw materials. [Item 1-3-7] A method for producing cells, comprising the step of culturing cells using the culture medium according to any one of the above items. [Item 1-3-8] The method according to any one of the above items, wherein the culturing step comprises culturing under conditions that satisfy at least one selected from the group consisting of a specific range of cell numbers, a specific container, and a specific temperature. [Item 1-3-8A] The range of cell numbers is such that MSCs are not seeded at low densities, preferably 500 cells / cm². ２ ~20000 cells / cm ２ That's good enough, and more preferably, 500-1000 / cm ２ ~10,000 / cm ２ For example, 2000 cells / cm² ２The method according to any one of the above items, although often seeded before or after. [Item 1-3-8B] The method according to any one of the above items, wherein the range of the cell number is a specific value such as 1500 cells / well at the time of screening, and / or is carried out under conditions such as 4500 cells / well which are estimated to become confluent. [Item 1-3-8C] The method according to any one of the above items, wherein the specific container is a container of a suitable material (including with or without coating). [Item 1-3-8D] The method according to any one of the above items, wherein the specific temperature is a suitable temperature (25-37°C, or 30°C, etc.). [Item 1-3-9] A cell culture apparatus comprising a culture medium according to any one of the above items or means for providing the culture medium, a container capable of containing the culture medium, and means for providing conditions for culturing cells as necessary. [Item 1-3-10] A method for preserving cells, comprising the step of preserving cells using the culture medium according to any one of the above items. [Item 1-3-11] A composition for preserving cells, comprising the component described in any one of the above items. [Item 1-3-12] A cell-containing composition comprising the component described in any one of the above items and cells. [Item 1-3-13] Cells cultured using the component described in any one of the above items. [Item 1-3-13A] The cells described in any one of the above items, wherein the expression of at least one selected from the group consisting of CD44, CD73, CD90, and CD105 is not significantly reduced. [Item 1-3-13B] A cell population comprising the cells described in any of the above items, wherein the expression of at least one selected from the group consisting of CD44, CD73, CD90, and CD105 is not significantly reduced even after long-term passage. [Item 1-3-13C] The marker is at least one of CD44, CD73, CD90, and CD105, and at least one of these does not decrease. Typically, CD44 does not decrease, but the others often decrease, and it is a desirable and advantageous characteristic that CD73 and CD90, or CD44, CD90 and CD105, do not decrease, in any one of the above items, a cell or cell population.[Item 1-3-13D] Cells or cell populations according to any one of the above items, in which high expression of CD90 is maintained. [Item 1-3-13E] Cells or cell populations according to any one of the above items, in which the expression of at least one of the markers (preferably at least one of CD73, CD90, and CD105) is not significantly reduced when the number of passages is 5 or more, preferably 6 or more, and more preferably 7 or more. [Item 1-3-13F] Cells or cell populations according to any one of the above items, in which the expression of at least one of the markers (preferably at least one of CD73, CD90, and CD105) is not significantly reduced when the number of passages is 8 or more. [Item 1-3-13G] Cells or cell populations according to any one of the above items, in which differentiation ability is maintained when the number of passages is 5 or more, preferably 6 or more, and more preferably 7 or more. [Item 1-3-13H] Cells or cell populations according to any one of the above items, in which differentiation ability is maintained when the number of passages is 8 or more. [Item 1-3-13I] A cell or cell population according to any one of the above items, wherein the expression of CD90, CD44, and CD73 is maintained at 70% or more, usually 80% or more, preferably 90% or more, and more preferably 95% or more, respectively, before passage. [Item 1-3-13J] A cell or cell population according to any one of the above items, wherein the expression of CD105 is maintained at 30% or more, usually 40% or more, preferably 50% or more, and more preferably 70% or more, before passage. [Item 1-3-13AA] The cell according to item 1-3-13, wherein the properties of the cell are improved (for example, the ability to differentiate into cartilage is enhanced). [Item 1-3-13AB] A cell population containing about 50% or more, about 60% or more, about 70% or more, about 80% or more, about 90% or more, or more, of the cells according to any one of items 1-3-13 to 1-13AA. [Item 1-3-14] A pharmaceutical product comprising cells described in any one of the above items. [Item 1-3-15] A method for differentiating cells, comprising the step of culturing cells obtained using a culture medium described in any one of the above items under conditions that induce differentiation. [Item 1-3-15A] The method according to any one of the above items, wherein the culture comprises culturing the cells after proliferation using a different differentiation medium while confirming the differentiation ability.[Item 1-3-15B] The method according to any one of the above items, wherein the conditions under which the differentiation is induced also include differentiation conditions that are commonly used for MSCs. [Item 1-3-15C] The method according to any one of the above items, wherein the cells are less likely to become cartilage and / or have high cartilage differentiation potential. [Item 1-3-16] Differentiated cells obtained by differentiating stem cells by the method according to any one of the above items. [Item 1-3-17] A method for treating or preventing a subject, comprising the step of administering an effective amount of the cells or the pharmaceutical described in any one of the above items to a subject in need thereof. [Item 1-3-18] A method for quality control of stem cells, comprising determining whether they have a coefficient C correlated with the culture medium described in any one of the above items. [Item 1-3-18A] The method according to any one of the above items, wherein the coefficient C, in the case of cells, includes a coefficient relating to cell markers. [Item 1-3-18B] The method according to any one of the above items, wherein the cell marker includes common markers, such as cell markers related to proliferation. [Item 1-3-18C] The method according to any of the above items, wherein the cell marker includes markers described herein, for example, those that enhance differentiation ability or those that are expressed over a long period of time. [Item 1-3-18D] The method according to any of the above items, wherein the marker, in the case of a culture medium, includes information relating to the identification of the component itself. [Item 1-3-19] A system for culturing cells, comprising: 1) a cell culture section for containing cells intended for culture; 2) a culture medium supply section for providing a culture medium or raw materials according to any one of the above items; and 3) a cell culture condition adjustment section for adjusting culture conditions as necessary. [Item 1-3-20] A culture medium suitable for transporting cells containing culture medium components according to any one of the above items. [Item 1-3-21] A method for managing cell culture for cells suitable for culturing using a culture medium according to any one of the above items, comprising: 1) providing information relating to the culture medium for cells provided by the user; 2) assigning identifiable labels to the cells and the culture medium; and 3) managing whether the cells and the culture medium are compatible by referring to the labels.

[0015] [Item 1-3-22] The use of (1) and (3) of Item 1-3-1 for culturing cells (preferably mesenchymal stem cells). [Item 1-3-23] The use of Item 1-3-22 for culturing cells (preferably mesenchymal stem cells), comprising the features of any one of Items 1-3-2 to 1-3-6C. [Item 1-3-24] A method for maintaining or improving the quality of cells (preferably mesenchymal stem cells) (preferably, the expression level of at least one selected from the group consisting of CD44, CD73, CD90 and CD105, and / or differentiation potential after long-term passage), comprising the step of contacting the cells in a culture medium containing the components of Item 1-3-1. [Item 1-3-25] The method of Item 1-3-24, comprising the features of any one of Items 1-3-2 to 1-3-6C. [Item 1-3-26] Cells or cell populations described in any one of items 1-3-13 to 1-3-13AB, or differentiated cells or cell populations described in item 1-3-16, for use in treatment. [Item 1-3-27] Cells or cell populations described in any one of items 1-3-13 to 1-3-13AB, or differentiated cells or cell populations described in item 1-3-16, for use in regenerative medicine or in the treatment of cartilage diseases. [Item 1-3-28] Use of cells or cell populations described in any one of items 1-3-13 to 1-3-13AB, or differentiated cells or cell populations described in item 1-3-16, in the manufacture of drugs for regenerative medicine or in the treatment of cartilage diseases. [Item 1-3-29] A method for performing regenerative medicine or treating cartilage disease in a subject in need thereof, comprising the step of administering to the subject an effective amount of cells or cell populations described in any one of items 1-3-13 to 1-3-13AB, or differentiated cells or cell populations described in item 1-3-16.

[0016] Form 1-4 [Item 1-4-1] A culture medium comprising (1) and (4). [Item 1-4-2] A culture medium according to any of the above items, comprising (1') and (4'). [Item 1-4-3] A culture medium according to any one of the above items, comprising (1") and (4"). [Item 1-4-4] A culture medium according to any one of the above items, comprising (1A) and (4A). [Item 1-4-5] A culture medium according to any one of the above items, comprising (1B) and (4B). [Item 1-4-5A] A culture medium according to any one of the above items, comprising one or more of (1), (1'), (1"), (1A) and (1B), and one or more of (4), (4'), (4"), (4A) and (4B). [Item 1-4-6] A culture medium according to any one of the above items, for culturing various stem cells such as mesenchymal stem cells. [Item 1-4-6A] A culture medium according to any one of the above items, for culturing mesenchymal stem cells. [Item 1-4-6B] A serum-free culture medium according to any one of the above items. [Item 1-4-6C] A culture medium according to any one of the above items, which does not contain heterologous raw materials. [Item 1-4-7] A method for producing cells, comprising the step of culturing cells using the culture medium according to any one of the above items. [Item 1-4-8] The method according to any one of the above items, wherein the culturing step comprises culturing under conditions that satisfy at least one selected from the group consisting of a specific range of cell numbers, a specific container, and a specific temperature. [Item 1-4-8A] The range of cell numbers is such that MSCs are not seeded at low densities, preferably 500 cells / cm². ２ ~20000 cells / cm ２ That's good enough, and more preferably, 500-1000 / cm ２ ~10,000 / cm ２ For example, 2000 cells / cm² ２The method according to any one of the above items, although often seeded before or after. [Item 1-4-8B] The method according to any one of the above items, wherein the range of the cell number is a specific value, such as 1500 cells / well, at the time of screening, and / or is carried out under conditions such as 4500 cells / well, which are estimated to become confluent. [Item 1-4-8C] The method according to any one of the above items, wherein the specific container is a container of a suitable material (including with or without coating). [Item 1-4-8D] The method according to any one of the above items, wherein the specific temperature is a suitable temperature (such as 25-37°C or 30°C). [Item 1-4-9] A cell culture apparatus comprising a culture medium according to any one of the above items or means for providing the culture medium, a container capable of containing the culture medium, and means for providing conditions for culturing cells as necessary. [Item 1-4-10] A method for preserving cells, comprising the step of preserving cells using the culture medium according to any one of the above items. [Item 1-4-11] A composition for preserving cells, comprising the component described in any one of the above items. [Item 1-4-12] A cell-containing composition comprising the component described in any one of the above items and cells. [Item 1-4-13] Cells cultured using the component described in any one of the above items. [Item 1-4-13A] The cells described in any one of the above items, wherein the expression of at least one selected from the group consisting of CD44, CD73, CD90, and CD105 is not significantly reduced. [Item 1-4-13B] A cell population comprising the cells described in any of the above items, wherein the expression of at least one selected from the group consisting of CD44, CD73, CD90, and CD105 is not significantly reduced even after long-term passage. [Item 1-4-13C] The marker is at least one of CD44, CD73, CD90, and CD105, and at least one of these does not decrease. Typically, CD44 does not decrease, but the others often decrease, and it is a desirable and advantageous characteristic that CD73 and CD90, or CD44, CD90 and CD105, do not decrease, in any one of the above items, a cell or cell population.[Item 1-4-13D] Cells or cell populations according to any one of the above items, in which high expression of CD90 is maintained. [Item 1-4-13E] Cells or cell populations according to any one of the above items, in which the expression of at least one of the markers (preferably at least one of CD73, CD90, and CD105) is not significantly reduced when the number of passages is 5 or more, preferably 6 or more, and more preferably 7 or more. [Item 1-4-13F] Cells or cell populations according to any one of the above items, in which the expression of at least one of the markers (preferably at least one of CD73, CD90, and CD105) is not significantly reduced when the number of passages is 8 or more. [Item 1-4-13G] Cells or cell populations according to any one of the above items, in which differentiation ability is maintained when the number of passages is 5 or more, preferably 6 or more, and more preferably 7 or more. [Item 1-4-13H] Cells or cell populations according to any one of the above items, in which differentiation ability is maintained when the number of passages is 8 or more. [Item 1-4-13I] A cell or cell population according to any one of the above items, wherein the expression of CD90, CD44, and CD73 is maintained at 70% or more, usually 80% or more, preferably 90% or more, and more preferably 95% or more, respectively, before passage. [Item 1-4-13J] A cell or cell population according to any one of the above items, wherein the expression of CD105 is maintained at 30% or more, usually 40% or more, preferably 50% or more, and more preferably 70% or more, before passage. [Item 1-4-13AA] The cell according to Item 1-4-13, wherein the cell properties are improved (for example, the ability to differentiate into cartilage is enhanced). [Item 1-4-13AB] A cell population containing about 50% or more, about 60% or more, about 70% or more, about 80% or more, about 90% or more, or more, of the cells according to any one of Items 1-4-13 to 1-4-13AA. [Item 1-4-14] A pharmaceutical product comprising cells described in any one of the above items. [Item 1-4-15] A method for differentiating cells, comprising the step of culturing cells obtained using a culture medium described in any one of the above items under conditions that induce differentiation. [Item 1-4-15A] The method according to any one of the above items, wherein the culture comprises culturing the cells after proliferation using a different differentiation medium while confirming the differentiation ability.[Item 1-4-15B] The method according to any one of the above items, wherein the conditions under which the differentiation is induced also include differentiation conditions that are commonly used for MSCs. [Item 1-4-15C] The method according to any one of the above items, wherein the cells are less likely to become cartilage and / or have high cartilage differentiation potential. [Item 1-4-16] Differentiated cells obtained by differentiating stem cells by the method according to any one of the above items. [Item 1-4-17] A method for treating or preventing a subject, comprising the step of administering an effective amount of the cells or the pharmaceutical described in any one of the above items to a subject in need thereof. [Item 1-4-18] A method for quality control of stem cells, comprising determining whether they have a coefficient C correlated with the culture medium described in any one of the above items. [Item 1-4-18A] The method according to any one of the above items, wherein the coefficient C, in the case of cells, includes a coefficient relating to cell markers. [Item 1-4-18B] The method according to any one of the above items, wherein the cell marker includes common markers, such as cell markers related to proliferation. [Item 1-4-18C] The method according to any of the above items, wherein the cell marker includes markers described herein, for example, those that enhance differentiation ability or those that are expressed over a long period of time. [Item 1-4-18D] The method according to any of the above items, wherein the marker, in the case of a culture medium, includes information relating to the identification of the component itself. [Item 1-4-19] A system for culturing cells, comprising: 1) a cell culture section for containing cells intended for culture; 2) a culture medium supply section for providing a culture medium or raw materials according to any of the above items; and 3) a cell culture condition adjustment section for adjusting culture conditions as necessary. [Item 1-4-20] A culture medium suitable for transporting cells containing culture medium components according to any of the above items. [Item 1-4-21] A method for managing cell culture for cells suitable for culturing using a culture medium according to any of the above items, comprising: 1) providing information relating to the culture medium for cells provided by the user; 2) assigning identifiable labels to the cells and the culture medium; and 3) managing whether the cells and the culture medium are compatible by referring to the labels.

[0017] [Item 1-4-22] The use of (1) and (4) of Item 1-4-1 for culturing cells (preferably mesenchymal stem cells). [Item 1-4-23] The use of Item 1-4-22 for culturing cells (preferably mesenchymal stem cells), comprising the features of any one of Items 1-4-2 to 1-4-6C. [Item 1-4-24] A method for maintaining or improving the quality of cells (preferably mesenchymal stem cells) (preferably, the expression level of at least one selected from the group consisting of CD44, CD73, CD90 and CD105, and / or differentiation potential after long-term passage), the method comprising the step of contacting the cells in a culture medium containing the components of Item 1-4-1. [Item 1-4-25] The method of Item 1-4-24, comprising the features of any one of Items 1-4-2 to 1-4-6C. [Item 1-4-26] Cells or cell populations described in any one of items 1-4-13 to 1-4-13AB, or differentiated cells or cell populations described in item 1-4-16, for use in treatment. [Item 1-4-27] Cells or cell populations described in any one of items 1-4-13 to 1-4-13AB, or differentiated cells or cell populations described in item 1-4-16, for use in regenerative medicine or in the treatment of cartilage diseases. [Item 1-4-28] Use of cells or cell populations described in any one of items 1-4-13 to 1-4-13AB, or differentiated cells or cell populations described in item 1-4-16, in the manufacture of drugs for regenerative medicine or in the treatment of cartilage diseases. [Item 1-4-29] A method for performing regenerative medicine or treating cartilage disease in a subject in need thereof, comprising the step of administering to the subject an effective amount of cells or cell populations described in any one of items 1-4-13 to 1-4-13AB, or differentiated cells or cell populations described in item 1-4-16.

[0018] Form 1-5 [Item 1-5-1] A culture medium comprising (1) and (5). [Item 1-5-2] A culture medium according to any of the above items, comprising (1') and (5'). [Item 1-5-3] A culture medium according to any one of the above items, comprising (1") and (5"). [Item 1-5-4] A culture medium according to any one of the above items, comprising (1A) and (5A). [Item 1-5-5] A culture medium according to any one of the above items, comprising (1B) and (5B). [Item 1-5-5A] A culture medium according to any one of the above items, comprising one or more of (1), (1'), (1"), (1A) and (1B), and one or more of (5), (5'), (5"), (5A) and (5B). [Item 1-5-6] A culture medium according to any one of the above items, for culturing various stem cells such as mesenchymal stem cells. [Item 1-5-6A] A culture medium according to any one of the above items, for culturing mesenchymal stem cells. [Item 1-5-6B] A serum-free culture medium according to any one of the above items. [Item 1-5-6C] A culture medium according to any one of the above items, which does not contain heterologous raw materials. [Item 1-5-7] A method for producing cells, comprising the step of culturing cells using the culture medium according to any one of the above items. [Item 1-5-8] The method according to any one of the above items, wherein the culturing step comprises culturing under conditions that satisfy at least one selected from the group consisting of a specific range of cell numbers, a specific container, and a specific temperature. [Item 1-5-8A] The range of cell numbers is such that MSCs are not seeded at low densities, preferably 500 cells / cm². ２ ~20000 cells / cm ２ That's good enough, and more preferably, 500-1000 / cm ２ ~10,000 / cm ２ For example, 2000 cells / cm² ２The method according to any one of the above items, although often seeded before or after. [Item 1-5-8B] The method according to any one of the above items, wherein the range of the cell number is a specific value, such as 1500 cells / well, at the time of screening, and / or is carried out under conditions such as 4500 cells / well, which are estimated to become confluent. [Item 1-5-8C] The method according to any one of the above items, wherein the specific container is a container of a suitable material (including with or without coating). [Item 1-5-8D] The method according to any one of the above items, wherein the specific temperature is a suitable temperature (such as 25-37°C or 30°C). [Item 1-5-9] A cell culture apparatus comprising a culture medium according to any one of the above items or means for providing the culture medium, a container capable of containing the culture medium, and means for providing conditions for culturing cells as necessary. [Item 1-5-10] A method for preserving cells, comprising the step of preserving cells using the culture medium according to any one of the above items. [Item 1-5-11] A composition for preserving cells, comprising the component described in any one of the above items. [Item 1-5-12] A cell-containing composition comprising the component described in any one of the above items and cells. [Item 1-5-13] Cells cultured using the component described in any one of the above items. [Item 1-5-13A] The cells described in any one of the above items, wherein the expression of at least one selected from the group consisting of CD44, CD73, CD90, and CD105 is not significantly reduced. [Item 1-5-13B] A cell population comprising the cells described in any of the above items, wherein the expression of at least one selected from the group consisting of CD44, CD73, CD90, and CD105 is not significantly reduced even after long-term passage. [Item 1-5-13C] The marker is at least one of CD44, CD73, CD90, and CD105, and at least one of these does not decrease. Typically, CD44 does not decrease, but the others often decrease, and it is a desirable and advantageous characteristic that CD73 and CD90, or CD44, CD90 and CD105, do not decrease, in any one of the above items, a cell or cell population.[Item 1-5-13D] Cells or cell populations according to any one of the above items, in which high expression of CD90 is maintained. [Item 1-5-13E] Cells or cell populations according to any one of the above items, in which the expression of at least one of the markers (preferably at least one of CD73, CD90, and CD105) is not significantly reduced when the number of passages is 5 or more, preferably 6 or more, and more preferably 7 or more. [Item 1-5-13F] Cells or cell populations according to any one of the above items, in which the expression of at least one of the markers (preferably at least one of CD73, CD90, and CD105) is not significantly reduced when the number of passages is 8 or more. [Item 1-5-13G] Cells or cell populations according to any one of the above items, in which differentiation ability is maintained when the number of passages is 5 or more, preferably 6 or more, and more preferably 7 or more. [Item 1-5-13H] Cells or cell populations according to any one of the above items, in which differentiation ability is maintained when the number of passages is 8 or more. [Item 1-5-13I] A cell or cell population according to any one of the above items, wherein the expression of CD90, CD44, and CD73 is maintained at 70% or more, usually 80% or more, preferably 90% or more, and more preferably 95% or more, respectively, before passage. [Item 1-5-13J] A cell or cell population according to any one of the above items, wherein the expression of CD105 is maintained at 30% or more, usually 40% or more, preferably 50% or more, and more preferably 70% or more, before passage. [Item 1-5-13AA] The cell according to item 1-5-13, wherein the cell properties are improved (for example, cartilage differentiation ability is enhanced). [Item 1-5-13AB] A cell population containing about 50% or more, about 60% or more, about 70% or more, about 80% or more, about 90% or more, or more of the cells according to any one of items 1-5-13 to 1-5-13AA. [Item 1-5-14] A pharmaceutical product comprising cells described in any one of the above items. [Item 1-5-15] A method for differentiating cells, comprising the step of culturing cells obtained using a culture medium described in any one of the above items under conditions that induce differentiation. [Item 1-5-15A] The method according to any one of the above items, wherein the culture comprises culturing the cells after proliferation using a different differentiation medium while confirming the differentiation ability.[Item 1-5-15B] The method according to any one of the above items, wherein the conditions under which the differentiation is induced also include differentiation conditions that are commonly used for MSCs. [Item 1-5-15C] The method according to any one of the above items, wherein the cells are less likely to become cartilage and / or have high cartilage differentiation potential. [Item 1-5-16] Differentiated cells obtained by differentiating stem cells by the method according to any one of the above items. [Item 1-5-17] A method for treating or preventing a subject, comprising the step of administering an effective amount of the cells or the pharmaceutical described in any one of the above items to a subject in need thereof. [Item 1-5-18] A method for quality control of stem cells, comprising determining whether they have a coefficient C correlated with the culture medium described in any one of the above items. [Item 1-5-18A] The method according to any one of the above items, wherein the coefficient C, in the case of cells, includes a coefficient relating to cell markers. [Item 1-5-18B] The method according to any one of the above items, wherein the cell marker includes common markers, such as cell markers related to proliferation. [Item 1-5-18C] The method according to any of the above items, wherein the cell marker includes markers described herein, for example, those that enhance differentiation ability or those that are expressed over a long period of time. [Item 1-5-18D] The method according to any of the above items, wherein the marker, in the case of a culture medium, includes information relating to the identification of the component itself. [Item 1-5-19] A system for culturing cells, comprising: 1) a cell culture section for containing cells intended for culture; 2) a medium supply section for providing a culture medium or raw materials according to any one of the above items; and 3) a cell culture condition adjustment section for adjusting culture conditions as necessary. [Item 1-5-20] A culture medium suitable for transporting cells containing the culture medium components according to any one of the above items. [Item 1-5-21] A method for managing cell culture for cells suitable for culturing using a culture medium according to any one of the above items, comprising: 1) providing information relating to the culture medium for cells provided by the user; 2) assigning identifiable labels to the cells and the culture medium; and 3) managing whether the cells and the culture medium are compatible by referring to the labels.

[0019] [Item 1-5-22] The use of (1) and (5) of Item 1-5-1 for culturing cells (preferably mesenchymal stem cells). [Item 1-5-23] The use of Item 1-5-22 for culturing cells (preferably mesenchymal stem cells), comprising the features of any one of Items 1-5-2 to 1-5-6C. [Item 1-5-24] A method for maintaining or improving the quality of cells (preferably mesenchymal stem cells) (preferably, the expression level of at least one selected from the group consisting of CD44, CD73, CD90 and CD105, and / or differentiation potential after long-term passage), comprising the step of contacting the cells in a culture medium containing the components of Item 1-5-1. [Item 1-5-25] The method of Item 1-5-24, comprising the features of any one of Items 1-5-2 to 1-5-6C. [Item 1-5-26] Cells or cell populations described in any one of items 1-5-13 to 1-5-13AB, or differentiated cells or cell populations described in item 1-5-16, for use in treatment. [Item 1-5-27] Cells or cell populations described in any one of items 1-5-13 to 1-5-13AB, or differentiated cells or cell populations described in item 1-5-16, for use in regenerative medicine or in the treatment of cartilage diseases. [Item 1-5-28] Use of cells or cell populations described in any one of items 1-5-13 to 1-5-13AB, or differentiated cells or cell populations described in item 1-5-16, in the manufacture of drugs for regenerative medicine or in the treatment of cartilage diseases. [Item 1-5-29] A method for performing regenerative medicine or treating cartilage disease in a subject in need thereof, comprising the step of administering to the subject an effective amount of cells or cell populations described in any one of items 1-5-13 to 1-5-13AB, or differentiated cells or cell populations described in item 1-5-16.

[0020] Form 1-6 [Item 1-6-1] A culture medium comprising (1) and (6). [Item 1-6-2] A culture medium according to any of the above items, comprising (1') and (6'). [Item 1-6-3] A culture medium according to any one of the above items, comprising (1") and (6"). [Item 1-6-4] A culture medium according to any one of the above items, comprising (1A) and (6A). [Item 1-6-5] A culture medium according to any one of the above items, comprising (1B) and (6B). [Item 1-6-6A] A culture medium according to any one of the above items, comprising one or more of (1), (1'), (1"), (1A) and (1B), and one or more of (6), (6'), (6"), (6A) and (6B). [Item 1-6-6] A culture medium according to any one of the above items, for culturing various stem cells such as mesenchymal stem cells. [Item 1-6-6A] A culture medium according to any one of the above items, for culturing mesenchymal stem cells. [Item 1-6-6B] A serum-free culture medium according to any one of the above items. [Item 1-6-6C] A culture medium according to any one of the above items, which does not contain heterologous raw materials. [Item 1-6-7] A method for producing cells, comprising the step of culturing cells using the culture medium according to any one of the above items. [Item 1-6-8] The method according to any one of the above items, wherein the culturing step comprises culturing under conditions that satisfy at least one selected from the group consisting of a specific range of cell numbers, a specific container, and a specific temperature. [Item 1-6-8A] The range of cell numbers is such that MSCs are not seeded at low densities, preferably 500 cells / cm². ２ ~20000 cells / cm ２ That's good enough, and more preferably, 500-1000 / cm ２ ~10,000 / cm ２ For example, 2000 cells / cm² ２The method according to any one of the above items, although often seeded before or after. [Item 1-6-8B] The method according to any one of the above items, wherein the range of the cell number is adopted at the time of screening, such as 1500 cells / well, and / or carried out under conditions such as 4500 cells / well which are estimated to become confluent. [Item 1-6-8C] The method according to any one of the above items, wherein the specific container is a container of a suitable material (including with or without coating). [Item 1-6-8D] The method according to any one of the above items, wherein the specific temperature is a suitable temperature (such as 25-37°C or 30°C). [Item 1-6-9] A cell culture apparatus comprising a culture medium according to any one of the above items or means for providing the culture medium, a container capable of containing the culture medium, and means for providing conditions for culturing cells as necessary. [Item 1-6-10] A method for preserving cells, comprising the step of preserving cells using the culture medium according to any one of the above items. [Item 1-6-11] A composition for preserving cells, comprising the component described in any one of the above items. [Item 1-6-12] A cell-containing composition comprising the component described in any one of the above items and cells. [Item 1-6-13] Cells cultured using the component described in any one of the above items. [Item 1-6-13A] The cells described in any one of the above items, wherein the expression of at least one selected from the group consisting of CD44, CD73, CD90, and CD105 is not significantly reduced. [Item 1-6-13B] A cell population comprising the cells described in any of the above items, wherein the expression of at least one selected from the group consisting of CD44, CD73, CD90, and CD105 is not significantly reduced even after long-term passage. [Item 1-6-13C] The marker is at least one of CD44, CD73, CD90, and CD105, and at least one of these does not decrease. Typically, CD44 does not decrease, but the others often decrease, and it is a desirable and advantageous characteristic that CD73 and CD90, or CD44, CD90 and CD105, do not decrease, in any one of the above items, a cell or cell population.[Item 1-6-13D] Cells or cell populations according to any one of the above items, in which high expression of CD90 is maintained. [Item 1-6-13E] Cells or cell populations according to any one of the above items, in which the expression of at least one of the markers (preferably at least one of CD73, CD90, and CD105) is not significantly reduced when the number of passages is 5 or more, preferably 6 or more, and more preferably 7 or more. [Item 1-6-13F] Cells or cell populations according to any one of the above items, in which the expression of at least one of the markers (preferably at least one of CD73, CD90, and CD105) is not significantly reduced when the number of passages is 8 or more. [Item 1-6-13G] Cells or cell populations according to any one of the above items, in which differentiation ability is maintained when the number of passages is 5 or more, preferably 6 or more, and more preferably 7 or more. [Item 1-6-13H] Cells or cell populations according to any one of the above items, in which differentiation ability is maintained when the number of passages is 8 or more. [Item 1-6-13I] A cell or cell population according to any one of the above items, wherein the expression of CD90, CD44, and CD73 is maintained at 70% or more, usually 80% or more, preferably 90% or more, and more preferably 95% or more, respectively, before passage. [Item 1-6-13J] A cell or cell population according to any one of the above items, wherein the expression of CD105 is maintained at 30% or more, usually 40% or more, preferably 50% or more, and more preferably 70% or more, before passage. [Item 1-6-13AA] The cell according to Item 1-6-13, wherein the properties of the cell are improved (for example, the ability to differentiate into cartilage is enhanced). [Item 1-6-13AB] A cell population containing about 50% or more, about 60% or more, about 70% or more, about 80% or more, about 90% or more, or more of the cells according to any one of Items 1-6-13 to 1-6-13AA. [Item 1-6-14] A pharmaceutical product comprising cells described in any one of the above items. [Item 1-6-15] A method for differentiating cells, comprising the step of culturing cells obtained using a culture medium described in any one of the above items under conditions that induce differentiation. [Item 1-6-15A] The method according to any one of the above items, wherein the culture comprises culturing the cells after proliferation using a different differentiation medium while confirming their differentiation ability.[Item 1-6-15B] The method according to any one of the above items, wherein the conditions under which the differentiation is induced also include differentiation conditions that are commonly used for MSCs. [Item 1-6-15C] The method according to any one of the above items, wherein the cells are less likely to become cartilage and / or have high cartilage differentiation potential. [Item 1-6-16] Differentiated cells obtained by differentiating stem cells by the method according to any one of the above items. [Item 1-6-17] A method for treating or preventing a subject, comprising the step of administering an effective amount of the cells or the pharmaceutical described in any one of the above items to a subject in need thereof. [Item 1-6-18] A method for quality control of stem cells, comprising determining whether they have a coefficient C correlated with the culture medium described in any one of the above items. [Item 1-6-18A] The method according to any one of the above items, wherein the coefficient C, in the case of cells, includes a coefficient relating to cell markers. [Item 1-6-18B] The method according to any one of the above items, wherein the cell marker includes common markers, such as cell markers related to proliferation. [Item 1-6-18C] The method according to any of the above items, wherein the cell marker includes markers described herein, such as those that enhance differentiation ability or those that are expressed over a long period of time. [Item 1-6-18D] The method according to any of the above items, wherein the marker, in the case of a culture medium, includes information relating to the identification of the component itself. [Item 1-6-19] A system for culturing cells, comprising: 1) a cell culture section for containing cells intended for culture; 2) a culture medium supply section for providing a culture medium or raw materials according to any of the above items; and 3) a cell culture condition adjustment section for adjusting culture conditions as necessary. [Item 1-6-20] A culture medium suitable for transporting cells containing culture medium components according to any of the above items. [Item 1-6-21] A method for managing cell culture for cells suitable for culturing using a culture medium according to any of the above items, comprising: 1) providing information relating to the culture medium for cells provided by the user; 2) assigning identifiable labels to the cells and the culture medium; and 3) managing whether the cells and the culture medium are compatible by referring to the labels.

[0021] [Item 1-6-22] The use of (1) and (6) described in Item 1-6-1 for culturing cells (preferably mesenchymal stem cells). [Item 1-6-23] The use of Item 1-6-22 for culturing cells (preferably mesenchymal stem cells), comprising the features of any one of Items 1-6-2 to 1-6-6C. [Item 1-6-24] A method for maintaining or improving the quality of cells (preferably mesenchymal stem cells) (preferably, at least one expression level selected from the group consisting of CD44, CD73, CD90 and CD105, and / or differentiation potential after long-term passage), comprising the step of contacting the cells in a culture medium containing the components described in Item 1-6-1. [Item 1-6-25] The method according to Item 1-6-24, comprising the features of any one of Items 1-6-2 to 1-6-6C. [Item 1-6-26] Cells or cell populations described in any one of items 1-6-13 to 1-6-13AB, or differentiated cells or cell populations described in item 1-6-16, for use in treatment. [Item 1-6-27] Cells or cell populations described in any one of items 1-6-13 to 1-6-13AB, or differentiated cells or cell populations described in item 1-6-16, for use in regenerative medicine or in the treatment of cartilage diseases. [Item 1-6-28] Use of cells or cell populations described in any one of items 1-6-13 to 1-6-13AB, or differentiated cells or cell populations described in item 1-6-16, in the manufacture of drugs for regenerative medicine or in the treatment of cartilage diseases. [Item 1-6-29] A method for performing regenerative medicine or treating cartilage disease in a subject in need thereof, comprising the step of administering to the subject an effective amount of cells or cell populations described in any one of items 1-6-13 to 1-6-13AB, or differentiated cells or cell populations described in item 1-6-16.

[0022] Form 1-7 [Item 1-7-1] A culture medium comprising (1) and (7). [Item 1-7-2] A culture medium according to any of the above items, comprising (1') and (7'). [Item 1-7-3] A culture medium according to any one of the above items, comprising (1") and (7"). [Item 1-7-4] A culture medium according to any one of the above items, comprising (1A) and (7A). [Item 1-7-5] A culture medium according to any one of the above items, comprising (1B) and (7B). [Item 1-7-5A] A culture medium according to any one of the above items, comprising one or more of (1), (1'), (1"), (1A) and (1B), and one or more of (7), (7'), (7"), (7A) and (7B). [Item 1-7-6] A culture medium according to any one of the above items, for culturing various stem cells such as mesenchymal stem cells. [Item 1-7-6] A culture medium according to any one of the above items, for culturing various stem cells such as mesenchymal stem cells. [Item 1-7-6A] A culture medium according to any one of the above items, for culturing mesenchymal stem cells. [Item 1-7-6B] A serum-free culture medium according to any one of the above items. [Item 1-7-6C] A culture medium according to any one of the above items, which does not contain heterologous raw materials. [Item 1-7-7] A method for producing cells, comprising the step of culturing cells using the culture medium according to any one of the above items. [Item 1-7-8] The method according to any one of the above items, wherein the culturing step comprises culturing under conditions that satisfy at least one selected from the group consisting of a specific range of cell numbers, a specific container, and a specific temperature. [Item 1-7-8A] The range of cell numbers is such that MSCs are not seeded at low density, preferably 500 cells / cm². ２ ~20000 cells / cm ２ That's good enough, and more preferably, 500-1000 / cm ２ ~10,000 / cm ２ For example, 2000 cells / cm² ２The method according to any one of the above items, although often seeded before or after. [Item 1-7-8B] The method according to any one of the above items, wherein the range of the cell number is a specific value such as 1500 cells / well at the time of screening, and / or is carried out under conditions such as 4500 cells / well which are estimated to become confluent. [Item 1-7-8C] The method according to any one of the above items, wherein the specific container is a container of a suitable material (including with or without coating). [Item 1-7-8D] The method according to any one of the above items, wherein the specific temperature is a suitable temperature (25-37°C, or 30°C, etc.). [Item 1-7-9] A cell culture apparatus comprising a culture medium according to any one of the above items or means for providing the culture medium, a container capable of containing the culture medium, and means for providing conditions for culturing cells as necessary. [Item 1-7-10] A method for preserving cells, comprising the step of preserving cells using the culture medium according to any one of the above items. [Item 1-7-11] A composition for preserving cells, comprising the component described in any one of the above items. [Item 1-7-12] A cell-containing composition comprising the component described in any one of the above items and cells. [Item 1-7-13] Cells cultured using the component described in any one of the above items. [Item 1-7-13A] The cells described in any one of the above items, wherein the expression of at least one selected from the group consisting of CD44, CD73, CD90, and CD105 is not significantly reduced. [Item 1-7-13B] A cell population comprising the cells described in any of the above items, wherein the expression of at least one selected from the group consisting of CD44, CD73, CD90, and CD105 is not significantly reduced even after long-term passage. [Item 1-7-13C] The marker is at least one of CD44, CD73, CD90, and CD105, and at least one of these does not decrease. Typically, CD44 does not decrease, but the others often decrease, and it is a desirable and advantageous characteristic that CD73 and CD90, or CD44, CD90 and CD105, do not decrease, in any one of the above items, a cell or cell population.[Item 1-7-13D] Cells or cell populations according to any one of the above items, in which high expression of CD90 is maintained. [Item 1-7-13E] Cells or cell populations according to any one of the above items, in which the expression of at least one of the markers (preferably at least one of CD73, CD90, and CD105) is not significantly reduced when the number of passages is 5 or more, preferably 6 or more, and more preferably 7 or more. [Item 1-7-13F] Cells or cell populations according to any one of the above items, in which the expression of at least one of the markers (preferably at least one of CD73, CD90, and CD105) is not significantly reduced when the number of passages is 8 or more. [Item 1-7-13G] Cells or cell populations according to any one of the above items, in which differentiation ability is maintained when the number of passages is 5 or more, preferably 6 or more, and more preferably 7 or more. [Item 1-7-13H] Cells or cell populations according to any one of the above items, in which differentiation ability is maintained when the number of passages is 8 or more. [Item 1-7-13I] A cell or cell population according to any one of the above items, wherein the expression of CD90, CD44, and CD73 is maintained at 70% or more, usually 80% or more, preferably 90% or more, and more preferably 95% or more, respectively, before passage. [Item 1-7-13J] A cell or cell population according to any one of the above items, wherein the expression of CD105 is maintained at 30% or more, usually 40% or more, preferably 50% or more, and more preferably 70% or more, before passage. [Item 1-7-13AA] The cell according to Item 1-7-13, wherein the cell properties are improved (for example, cartilage differentiation ability is enhanced). [Item 1-7-13AB] A cell population containing about 50% or more, about 60% or more, about 70% or more, about 80% or more, about 90% or more, or more of the cells according to any one of Items 1-7-13 to 1-7-13AA. [Item 1-7-14] A pharmaceutical product comprising cells described in any one of the above items. [Item 1-7-15] A method for differentiating cells, comprising the step of culturing cells obtained using a culture medium described in any one of the above items under conditions that induce differentiation. [Item 1-7-15A] The method according to any one of the above items, wherein the culture comprises culturing the cells after proliferation using a different differentiation medium while confirming their differentiation ability.[Item 1-7-15B] The method according to any one of the above items, wherein the conditions under which the differentiation is induced also include differentiation conditions that are commonly used for MSCs. [Item 1-7-15C] The method according to any one of the above items, wherein the cells are less likely to become cartilage and / or have high cartilage differentiation potential. [Item 1-7-16] Differentiated cells obtained by differentiating stem cells by the method according to any one of the above items. [Item 1-7-17] A method for treating or preventing a subject, comprising the step of administering an effective amount of the cells or the pharmaceutical described in any one of the above items to a subject in need thereof. [Item 1-7-18] A method for quality control of stem cells, comprising determining whether they have a coefficient C correlated with the culture medium described in any one of the above items. [Item 1-7-18A] The method according to any one of the above items, wherein the coefficient C, in the case of cells, includes a coefficient relating to cell markers. [Item 1-7-18B] The method according to any one of the above items, wherein the cell marker includes common markers, such as cell markers related to proliferation. [Item 1-7-18C] The method according to any of the above items, wherein the cell marker includes markers described herein, for example, those that enhance differentiation ability or those that are expressed over a long period of time. [Item 1-7-18D] The method according to any of the above items, wherein the marker, in the case of a culture medium, includes information relating to the identification of the component itself. [Item 1-7-19] A system for culturing cells, comprising: 1) a cell culture section for containing cells intended for culture; 2) a culture medium supply section for providing a culture medium or raw materials according to any one of the above items; and 3) a cell culture condition adjustment section for adjusting culture conditions as necessary. [Item 1-7-20] A culture medium suitable for transporting cells containing culture medium components according to any one of the above items. [Item 1-7-21] A method for managing cell culture for cells suitable for culturing using a culture medium according to any one of the above items, comprising: 1) providing information relating to the culture medium for cells provided by the user; 2) assigning identifiable labels to the cells and the culture medium; and 3) managing whether the cells and the culture medium are compatible by referring to the labels.

[0023] [Item 1-7-22] The use of (1) and (7) described in Item 1-7-1 for culturing cells (preferably mesenchymal stem cells). [Item 1-7-23] The use of Item 1-7-22 for culturing cells (preferably mesenchymal stem cells), comprising the features of any one of Items 1-7-2 to 1-7-6C. [Item 1-7-24] A method for maintaining or improving the quality of cells (preferably mesenchymal stem cells) (preferably, the expression level of at least one selected from the group consisting of CD44, CD73, CD90 and CD105, and / or differentiation potential after long-term passage), the method comprising the step of contacting the cells in a culture medium containing the components described in Item 1-7-1. [Item 1-7-25] The method according to Item 1-7-24, comprising the features of any one of Items 1-7-2 to 1-7-6C. [Item 1-7-26] Cells or cell populations described in any one of items 1-7-13 to 1-7-13AB, or differentiated cells or cell populations described in item 1-7-16, for use in treatment. [Item 1-7-27] Cells or cell populations described in any one of items 1-7-13 to 1-7-13AB, or differentiated cells or cell populations described in item 1-7-16, for use in regenerative medicine or in the treatment of cartilage diseases. [Item 1-7-28] Use of cells or cell populations described in any one of items 1-7-13 to 1-7-13AB, or differentiated cells or cell populations described in item 1-7-16, in the manufacture of drugs for regenerative medicine or in the treatment of cartilage diseases. [Item 1-7-29] A method for performing regenerative medicine or treating cartilage disease in a subject in need thereof, comprising the step of administering to the subject an effective amount of cells or cell populations described in any one of items 1-7-13 to 1-7-13AB, or differentiated cells or cell populations described in item 1-7-16.

[0024] Form 2-3 [Item 2-3-1] A culture medium comprising (2) and (3). [Item 2-3-2] A culture medium according to any of the above items, comprising (2') and (3'). [Item 2-3-3] A culture medium according to any one of the above items, comprising (2") and (3"). [Item 2-3-4] A culture medium according to any one of the above items, comprising (2A) and (3A). [Item 2-3-5] A culture medium according to any one of the above items, comprising (2B) and (3B). [Item 2-3-5A] A culture medium according to any one of the above items, comprising one or more of (2), (2'), (2"), (2A) and (2B), and one or more of (3), (3'), (3"), (3A) and (3B). [Item 2-3-6] A culture medium according to any one of the above items, for culturing various stem cells such as mesenchymal stem cells. [Item 2-3-6] A culture medium according to any one of the above items, for culturing various stem cells such as mesenchymal stem cells. [Item 2-3-6A] A culture medium according to any one of the above items, for culturing mesenchymal stem cells. [Item 2-3-6B] A serum-free culture medium according to any one of the above items. [Item 2-3-6C] A culture medium according to any one of the above items, which does not contain heterologous raw materials. [Item 2-3-7] A method for producing cells, comprising the step of culturing cells using the culture medium according to any one of the above items. [Item 2-3-8] The method according to any one of the above items, wherein the culturing step comprises culturing under conditions that satisfy at least one selected from the group consisting of a specific range of cell numbers, a specific container, and a specific temperature. [Item 2-3-8A] The range of cell numbers is such that MSCs are not seeded at low density, preferably 500 cells / cm². ２ ~20000 cells / cm ２ That's good enough, and more preferably, 500-1000 / cm ２ ~10,000 / cm ２ For example, 2000 cells / cm² ２The method according to any one of the above items, although often seeded before or after. [Item 2-3-8B] The method according to any one of the above items, wherein the range of the cell number is a specific value such as 1500 cells / well during screening, and / or is carried out under conditions such as 4500 cells / well which are estimated to become confluent. [Item 2-3-8C] The method according to any one of the above items, wherein the specific container is a container of a suitable material (including with or without coating). [Item 2-3-8D] The method according to any one of the above items, wherein the specific temperature is a suitable temperature (such as 25-37°C or 30°C). [Item 2-3-9] A cell culture apparatus comprising a culture medium according to any one of the above items or means for providing the culture medium, a container capable of containing the culture medium, and means for providing conditions for culturing cells as necessary. [Item 2-3-10] A method for preserving cells, comprising the step of preserving cells using the culture medium according to any one of the above items. [Item 2-3-11] A composition for preserving cells, comprising the component described in any one of the above items. [Item 2-3-12] A cell-containing composition comprising the component described in any one of the above items and cells. [Item 2-3-13] Cells cultured using the component described in any one of the above items. [Item 2-3-13A] The cells described in any one of the above items, wherein the expression of at least one selected from the group consisting of CD44, CD73, CD90, and CD105 is not significantly reduced. [Item 2-3-13B] A cell population comprising the cells described in any of the above items, wherein the expression of at least one selected from the group consisting of CD44, CD73, CD90, and CD105 is not significantly reduced even after long-term passage. [Item 2-3-13C] The marker is at least one of CD44, CD73, CD90, and CD105, and at least one of these does not decrease. Typically, CD44 does not decrease, but the others often decrease, and it is a desirable and advantageous characteristic that CD73 and CD90, or CD44, CD90 and CD105, do not decrease, in any one of the above items, a cell or cell population.[Item 2-3-13D] Cells or cell populations according to any one of the above items, in which high expression of CD90 is maintained. [Item 2-3-13E] Cells or cell populations according to any one of the above items, in which the expression of at least one of the markers (preferably at least one of CD73, CD90, and CD105) is not significantly reduced when the number of passages is 5 or more, preferably 6 or more, and more preferably 7 or more. [Item 2-3-13F] Cells or cell populations according to any one of the above items, in which the expression of at least one of the markers (preferably at least one of CD73, CD90, and CD105) is not significantly reduced when the number of passages is 8 or more. [Item 2-3-13G] Cells or cell populations according to any one of the above items, in which differentiation ability is maintained when the number of passages is 5 or more, preferably 6 or more, and more preferably 7 or more. [Item 2-3-13H] Cells or cell populations according to any one of the above items, in which differentiation ability is maintained when the number of passages is 8 or more. [Item 2-3-13I] A cell or cell population according to any one of the above items, wherein the expression of CD90, CD44, and CD73 is maintained at 70% or more, usually 80% or more, preferably 90% or more, and more preferably 95% or more, respectively, before passage. [Item 2-3-13J] A cell or cell population according to any one of the above items, wherein the expression of CD105 is maintained at 30% or more, usually 40% or more, preferably 50% or more, and more preferably 70% or more, before passage. [Item 2-3-13AA] The cell according to Item 2-3-13, wherein the cell properties are improved (for example, cartilage differentiation ability is enhanced). [Item 2-3-13AB] A cell population containing about 50% or more, about 60% or more, about 70% or more, about 80% or more, about 90% or more, or more, of the cells according to any one of Items 2-3-13 to 1-13AA. [Item 2-3-14] A pharmaceutical product comprising cells described in any one of the above items. [Item 2-3-15] A method for differentiating cells, comprising the step of culturing cells obtained using a culture medium described in any one of the above items under conditions that induce differentiation. [Item 2-3-15A] The method according to any one of the above items, wherein the culture comprises culturing the cells after proliferation using a different differentiation medium while confirming their differentiation ability.[Item 2-3-15B] The method according to any one of the above items, wherein the conditions under which the differentiation is induced also include differentiation conditions that are commonly used for MSCs. [Item 2-3-15C] The method according to any one of the above items, wherein the cells are less likely to become cartilage and / or have high cartilage differentiation potential. [Item 2-3-16] Differentiated cells obtained by differentiating stem cells by the method according to any one of the above items. [Item 2-3-17] A method for treating or preventing a subject, comprising the step of administering an effective amount of the cells or the pharmaceutical described in any one of the above items to a subject in need thereof. [Item 2-3-18] A method for quality control of stem cells, comprising determining whether they have a coefficient C correlated with the culture medium described in any one of the above items. [Item 2-3-18A] The method according to any one of the above items, wherein the coefficient C, in the case of cells, includes a coefficient relating to cell markers. [Item 2-3-18B] The method according to any one of the above items, wherein the cell marker includes common markers, such as cell markers related to proliferation. [Item 2-3-18C] The method according to any of the above items, wherein the cell marker includes markers described herein, for example, those that enhance differentiation ability or those that are expressed over a long period of time. [Item 2-3-18D] The method according to any of the above items, wherein the marker, in the case of a culture medium, includes information relating to the identification of the component itself. [Item 2-3-19] A system for culturing cells, comprising: 1) a cell culture section for containing cells intended for culture; 2) a medium supply section for providing a culture medium or raw materials according to any one of the above items; and 3) a cell culture condition adjustment section for adjusting culture conditions as necessary. [Item 2-3-20] A culture medium suitable for transporting cells containing culture medium components according to any one of the above items. [Item 2-3-21] A method for managing cell culture for cells suitable for culturing using a culture medium according to any one of the above items, comprising: 1) providing information relating to the culture medium for cells provided by the user; 2) assigning identifiable labels to the cells and the culture medium; and 3) managing whether the cells and the culture medium are compatible by referring to the labels.

[0025] [Item 2-3-22] The use of (2) and (3) of Item 2-3-1 for culturing cells (preferably mesenchymal stem cells). [Item 2-3-23] The use of Item 2-3-22 for culturing cells (preferably mesenchymal stem cells), comprising the features of any one of Items 2-3-2 to 2-3-6C. [Item 2-3-24] A method for maintaining or improving the quality of cells (preferably mesenchymal stem cells) (preferably, the expression level of at least one selected from the group consisting of CD44, CD73, CD90 and CD105, and / or differentiation potential after long-term passage), the method comprising the step of contacting the cells in a culture medium containing the components of Item 2-3-1. [Item 2-3-25] The method of Item 2-3-24, comprising the features of any one of Items 2-3-2 to 2-3-6C. [Item 2-3-26] Cells or cell populations described in any one of items 2-3-13 to 2-3-13AB, or differentiated cells or cell populations described in item 2-3-16, for use in treatment. [Item 2-3-27] Cells or cell populations described in any one of items 2-3-13 to 2-3-13AB, or differentiated cells or cell populations described in item 2-3-16, for use in regenerative medicine or in the treatment of cartilage diseases. [Item 2-3-28] Use of cells or cell populations described in any one of items 2-3-13 to 2-3-13AB, or differentiated cells or cell populations described in item 2-3-16, in the manufacture of drugs for regenerative medicine or in the treatment of cartilage diseases. [Item 2-3-29] A method for performing regenerative medicine or treating cartilage disease in a subject in need thereof, comprising the step of administering to the subject an effective amount of cells or cell populations described in any one of items 2-3-13 to 2-3-13AB, or differentiated cells or cell populations described in item 2-3-16.

[0026] Form 2-4 [Item 2-4-1] A culture medium comprising (2) and (4). [Item 2-4-2] A culture medium according to any of the above items, comprising (2') and (4'). [Item 2-4-3] A culture medium according to any one of the above items, comprising (2") and (4"). [Item 2-4-4] A culture medium according to any one of the above items, comprising (2A) and (4A). [Item 2-4-5] A culture medium according to any one of the above items, comprising (2B) and (4B). [Item 2-4-5A] A culture medium according to any one of the above items, comprising one or more of (2), (2'), (2"), (2A) and (2B), and one or more of (4), (4'), (4"), (4A) and (4B). [Item 2-4-6] A culture medium according to any one of the above items, for culturing various stem cells such as mesenchymal stem cells. [Item 2-4-6] A culture medium according to any one of the above items, for culturing various stem cells such as mesenchymal stem cells. [Item 2-4-6A] A culture medium according to any one of the above items, for culturing mesenchymal stem cells. [Item 2-4-6B] A serum-free culture medium according to any one of the above items. [Item 2-4-6C] A culture medium according to any one of the above items, which does not contain heterologous raw materials. [Item 2-4-7] A method for producing cells, comprising the step of culturing cells using the culture medium according to any one of the above items. [Item 2-4-8] The method according to any one of the above items, wherein the culturing step comprises culturing under conditions that satisfy at least one selected from the group consisting of a specific range of cell numbers, a specific container, and a specific temperature. [Item 2-4-8A] The range of cell numbers is such that MSCs are not seeded at low density, preferably 500 cells / cm². ２ ~20000 cells / cm ２ That's good enough, and more preferably, 500-1000 / cm ２ ~10,000 / cm ２ For example, 2000 cells / cm² ２The method according to any one of the above items, although often seeded before or after. [Item 2-4-8B] The method according to any one of the above items, wherein the range of the cell number is a specific value such as 1500 cells / well at the time of screening, and / or is carried out under conditions such as 4500 cells / well which are estimated to become confluent. [Item 2-4-8C] The method according to any one of the above items, wherein the specific container is a container of a suitable material (including with or without coating). [Item 2-4-8D] The method according to any one of the above items, wherein the specific temperature is a suitable temperature (25-37°C, or 30°C, etc.). [Item 2-4-9] A cell culture apparatus comprising a culture medium according to any one of the above items or means for providing the culture medium, a container capable of containing the culture medium, and means for providing conditions for culturing cells as necessary. [Item 2-4-10] A method for preserving cells, comprising the step of preserving cells using the culture medium according to any one of the above items. [Item 2-4-11] A composition for preserving cells, comprising the component described in any one of the above items. [Item 2-4-12] A cell-containing composition comprising the component described in any one of the above items and cells. [Item 2-4-13] Cells cultured using the component described in any one of the above items. [Item 2-4-13A] The cells described in any one of the above items, wherein the expression of at least one selected from the group consisting of CD44, CD73, CD90, and CD105 is not significantly reduced. [Item 2-4-13B] A cell population comprising the cells described in any of the above items, wherein the expression of at least one selected from the group consisting of CD44, CD73, CD90, and CD105 is not significantly reduced even after long-term passage. [Item 2-4-13C] The marker is at least one of CD44, CD73, CD90, and CD105, and at least one of these does not decrease. Typically, CD44 does not decrease, but the others often decrease, and it is a desirable and advantageous characteristic that CD73 and CD90, or CD44, CD90 and CD105, do not decrease, in any one of the above items, a cell or cell population.[Item 2-4-13D] Cells or cell populations according to any one of the above items, in which high expression of CD90 is maintained. [Item 2-4-13E] Cells or cell populations according to any one of the above items, in which the expression of at least one of the markers (preferably at least one of CD73, CD90, and CD105) is not significantly reduced when the number of passages is 5 or more, preferably 6 or more, and more preferably 7 or more. [Item 2-4-13F] Cells or cell populations according to any one of the above items, in which the expression of at least one of the markers (preferably at least one of CD73, CD90, and CD105) is not significantly reduced when the number of passages is 8 or more. [Item 2-4-13G] Cells or cell populations according to any one of the above items, in which differentiation ability is maintained when the number of passages is 5 or more, preferably 6 or more, and more preferably 7 or more. [Item 2-4-13H] Cells or cell populations according to any one of the above items, in which differentiation ability is maintained when the number of passages is 8 or more. [Item 2-4-13I] A cell or cell population according to any one of the above items, wherein the expression of CD90, CD44, and CD73 is maintained at 70% or more, usually 80% or more, preferably 90% or more, and more preferably 95% or more, respectively, before passage. [Item 2-4-13J] A cell or cell population according to any one of the above items, wherein the expression of CD105 is maintained at 30% or more, usually 40% or more, preferably 50% or more, and more preferably 70% or more, before passage. [Item 2-4-13AA] The cell according to item 2-4-13, wherein the cell properties are improved (for example, cartilage differentiation ability is enhanced). [Item 2-4-13AB] A cell population containing about 50% or more, about 60% or more, about 70% or more, about 80% or more, about 90% or more, or more of the cells according to any one of items 2-4-13 to 2-4-13AA. [Item 2-4-14] A pharmaceutical product comprising cells described in any one of the above items. [Item 2-4-15] A method for differentiating cells, comprising the step of culturing cells obtained using a culture medium described in any one of the above items under conditions that induce differentiation. [Item 2-4-15A] The method according to any one of the above items, wherein the culture comprises culturing the cells after proliferation using a different differentiation medium while confirming the differentiation ability.[Item 2-4-15B] The method according to any one of the above items, wherein the conditions under which the differentiation is induced also include differentiation conditions that are commonly used for MSCs. [Item 2-4-15C] The method according to any one of the above items, wherein the cells are less likely to become cartilage and / or have high cartilage differentiation potential. [Item 2-4-16] Differentiated cells obtained by differentiating stem cells by the method according to any one of the above items. [Item 2-4-17] A method for treating or preventing a subject, comprising the step of administering an effective amount of the cells or the pharmaceutical described in any one of the above items to a subject in need thereof. [Item 2-4-18] A method for quality control of stem cells, comprising determining whether they have a coefficient C correlated with the culture medium described in any one of the above items. [Item 2-4-18A] The method according to any one of the above items, wherein the coefficient C, in the case of cells, includes a coefficient relating to cell markers. [Item 2-4-18B] The method according to any one of the above items, wherein the cell marker includes common markers, such as cell markers associated with proliferation. [Item 2-4-18C] The method according to any of the above items, wherein the cell marker includes markers described herein, for example, those that enhance differentiation ability or those that exhibit long-term expression. [Item 2-4-18D] The method according to any of the above items, wherein the marker, in the case of a culture medium, includes information relating to the identification of the component itself. [Item 2-4-19] A system for culturing cells, comprising: 1) a cell culture section for containing cells intended for culture; 2) a culture medium supply section for providing a culture medium or its raw materials as described in any of the above items; and 3) a cell culture condition adjustment section for adjusting culture conditions as necessary. [Item 2-4-20] A culture medium suitable for transporting cells containing culture medium components as described in any of the above items. [Item 2-4-21] A method for managing cell culture for cells suitable for culturing using a culture medium as described in any of the above items, comprising: 1) providing information relating to the culture medium for cells provided by the user; 2) assigning identifiable labels to the cells and the culture medium; and 3) managing whether the cells and the culture medium are compatible by referring to the labels.

[0027] [Item 2-4-22] The use of (2) and (4) of Item 2-4-1 for culturing cells (preferably mesenchymal stem cells). [Item 2-4-23] The use of Item 2-4-22 for culturing cells (preferably mesenchymal stem cells), comprising the features of any one of Items 2-4-2 to 2-4-6C. [Item 2-4-24] A method for maintaining or improving the quality of cells (preferably mesenchymal stem cells) (preferably, the expression level of at least one selected from the group consisting of CD44, CD73, CD90 and CD105, and / or differentiation potential after long-term passage), comprising the step of contacting the cells in a culture medium containing the components of Item 2-4-1. [Item 2-4-25] The method of Item 2-4-24, comprising the features of any one of Items 2-4-2 to 2-4-6C. [Item 2-4-26] Cells or cell populations described in any one of items 2-4-13 to 2-4-13AB, or differentiated cells or cell populations described in item 2-4-16, for use in treatment. [Item 2-4-27] Cells or cell populations described in any one of items 2-4-13 to 2-4-13AB, or differentiated cells or cell populations described in item 2-4-16, for use in regenerative medicine or in the treatment of cartilage diseases. [Item 2-4-28] Use of cells or cell populations described in any one of items 2-4-13 to 2-4-13AB, or differentiated cells or cell populations described in item 2-4-16, in the manufacture of drugs for regenerative medicine or in the treatment of cartilage diseases. [Item 2-4-29] A method for performing regenerative medicine or treating cartilage disease in a subject in need thereof, comprising the step of administering to the subject an effective amount of cells or cell populations described in any one of items 2-4-13 to 2-4-13AB, or differentiated cells or cell populations described in item 2-4-16.

[0028] Form 2-5 [Item 2-5-1] A culture medium comprising (2) and (5). [Item 2-5-2] A culture medium according to any of the above items, comprising (2') and (5'). [Item 2-5-3] A culture medium according to any one of the above items, comprising (2") and (5"). [Item 2-5-4] A culture medium according to any one of the above items, comprising (2A) and (5A). [Item 2-5-5] A culture medium according to any one of the above items, comprising (2B) and (5B). [Item 2-5-5A] A culture medium according to any one of the above items, comprising one or more of (2), (2'), (2"), (2A) and (2B), and one or more of (5), (5'), (5"), (5A) and (5B). [Item 2-5-6] A culture medium according to any one of the above items, for culturing various stem cells such as mesenchymal stem cells. [Item 2-5-6A] A culture medium according to any one of the above items, for culturing mesenchymal stem cells. [Item 2-5-6B] A serum-free culture medium according to any one of the above items. [Item 2-5-6C] A culture medium according to any one of the above items, which does not contain heterologous raw materials. [Item 2-5-7] A method for producing cells, comprising the step of culturing cells using the culture medium according to any one of the above items. [Item 2-5-8] The method according to any one of the above items, wherein the culturing step comprises culturing under conditions that satisfy at least one selected from the group consisting of a specific range of cell numbers, a specific container, and a specific temperature. [Item 2-5-8A] The range of cell numbers is such that MSCs are not seeded at low densities, preferably 500 cells / cm². ２ ~20000 cells / cm ２ That's good enough, and more preferably, 500-1000 / cm ２ ~10,000 / cm ２ For example, 2000 cells / cm² ２[Item 2-5-8B] The method according to any one of the above items, wherein the seeding is often performed before and after the culture, but is not limited thereto. [Item 2-5-8B] The method according to any one of the above items, wherein the range of the cell number is adopted at the time of screening, such as 1500 cells / well, and / or performed under conditions such as 4500 cells / well, which are estimated to be confluent. [Item 2-5-8C] The method according to any one of the above items, wherein the specific container is a container of a suitable material (including with or without coating). [Item 2-5-8D] The method according to any one of the above items, wherein the specific temperature is a suitable temperature (such as 25-37°C or 30°C). [Item 2-5-9] A cell culture apparatus comprising a culture medium according to any one of the above items or means for providing the culture medium, a container capable of containing the culture medium, and means for providing conditions for culturing cells as necessary. [Item 2-5-10] A method for preserving cells, comprising the step of preserving cells using the culture medium according to any one of the above items. [Item 2-5-11] A composition for preserving cells, comprising the component described in any one of the above items. [Item 2-5-12] A cell-containing composition comprising the component described in any one of the above items and cells. [Item 2-5-13] Cells cultured using the component described in any one of the above items. [Item 2-5-13A] The cells described in any one of the above items, wherein the expression of at least one selected from the group consisting of CD44, CD73, CD90, and CD105 is not significantly reduced. [Item 2-5-13B] A cell population comprising the cells described in any of the above items, wherein the expression of at least one selected from the group consisting of CD44, CD73, CD90, and CD105 is not significantly reduced even after long-term passage. [Item 2-5-13C] The marker is at least one of CD44, CD73, CD90, and CD105, and at least one of these does not decrease. Typically, CD44 does not decrease, but the others often decrease, and it is a desirable and advantageous characteristic that CD73 and CD90, or CD44, CD90 and CD105, do not decrease, in any one of the above items, a cell or cell population.[Item 2-5-13D] Cells or cell populations according to any one of the above items, in which high expression of CD90 is maintained. [Item 2-5-13E] Cells or cell populations according to any one of the above items, in which the expression of at least one of the markers (preferably at least one of CD73, CD90, and CD105) is not significantly reduced when the number of passages is 5 or more, preferably 6 or more, and more preferably 7 or more. [Item 2-5-13F] Cells or cell populations according to any one of the above items, in which the expression of at least one of the markers (preferably at least one of CD73, CD90, and CD105) is not significantly reduced when the number of passages is 8 or more. [Item 2-5-13G] Cells or cell populations according to any one of the above items, in which differentiation ability is maintained when the number of passages is 5 or more, preferably 6 or more, and more preferably 7 or more. [Item 2-5-13H] Cells or cell populations according to any one of the above items, in which differentiation ability is maintained when the number of passages is 8 or more. [Item 2-5-13I] A cell or cell population according to any one of the above items, wherein the expression of CD90, CD44, and CD73 is maintained at 70% or more, usually 80% or more, preferably 90% or more, and more preferably 95% or more, respectively, before passage. [Item 2-5-13J] A cell or cell population according to any one of the above items, wherein the expression of CD105 is maintained at 30% or more, usually 40% or more, preferably 50% or more, and more preferably 70% or more, before passage. [Item 2-5-13AA] The cell according to item 2-5-13, wherein the cell properties are improved (for example, cartilage differentiation ability is enhanced). [Item 2-5-13AB] A cell population containing about 50% or more, about 60% or more, about 70% or more, about 80% or more, about 90% or more, or more of the cells according to any one of items 2-5-13 to 2-5-13AA. [Item 2-5-14] A pharmaceutical product comprising cells described in any one of the above items. [Item 2-5-15] A method for differentiating cells, comprising the step of culturing cells obtained using a culture medium described in any one of the above items under conditions that induce differentiation. [Item 2-5-15A] The method according to any one of the above items, wherein the culture comprises culturing the cells after proliferation using a different differentiation medium while confirming the differentiation ability.[Item 2-5-15B] The method according to any one of the above items, wherein the conditions under which the differentiation is induced also include differentiation conditions that are commonly used for MSCs. [Item 2-5-15C] The method according to any one of the above items, wherein the cells are less likely to become cartilage and / or have high cartilage differentiation potential. [Item 2-5-16] Differentiated cells obtained by differentiating stem cells by the method according to any one of the above items. [Item 2-5-17] A method for treating or preventing a subject, comprising the step of administering an effective amount of the cells or the pharmaceutical described in any one of the above items to a subject in need thereof. [Item 2-5-18] A method for quality control of stem cells, comprising determining whether they have a coefficient C correlated with the culture medium described in any one of the above items. [Item 2-5-18A] The method according to any one of the above items, wherein the coefficient C, in the case of cells, includes a coefficient relating to cell markers. [Item 2-5-18B] The method according to any one of the above items, wherein the cell marker includes common markers, such as cell markers associated with proliferation. [Item 2-5-18C] The method according to any of the above items, wherein the cell marker includes markers described herein, for example, those that enhance differentiation ability or those that are expressed over a long period of time. [Item 2-5-18D] The method according to any of the above items, wherein the marker, in the case of a culture medium, includes information relating to the identification of the component itself. [Item 2-5-19] A system for culturing cells, comprising: 1) a cell culture section for containing cells intended for culture; 2) a medium supply section for providing a culture medium or raw materials according to any of the above items; and 3) a cell culture condition adjustment section for adjusting culture conditions as necessary. [Item 2-5-20] A culture medium suitable for transporting cells containing culture medium components according to any of the above items. [Item 2-5-21] A method for managing cell culture for cells suitable for culturing using a culture medium according to any of the above items, comprising: 1) providing information relating to the culture medium for cells provided by the user; 2) assigning identifiable labels to the cells and the culture medium; and 3) managing whether the cells and the culture medium are compatible by referring to the labels.

[0029] [Item 2-5-22] The use of (2) and (5) described in Item 2-5-1 for culturing cells (preferably mesenchymal stem cells). [Item 2-5-23] The use of Item 2-5-22 for culturing cells (preferably mesenchymal stem cells), comprising the features of any one of Items 2-5-2 to 2-5-6C. [Item 2-5-24] A method for maintaining or improving the quality of cells (preferably mesenchymal stem cells) (preferably, the expression level of at least one selected from the group consisting of CD44, CD73, CD90 and CD105, and / or differentiation potential after long-term passage), comprising the step of contacting the cells in a culture medium containing the components described in Item 2-5-1. [Item 2-5-25] The method of Item 2-5-24, comprising the features of any one of Items 2-5-2 to 2-5-6C. [Item 2-5-26] Cells or cell populations described in any one of items 2-5-13 to 2-5-13AB, or differentiated cells or cell populations described in item 2-5-16, for use in treatment. [Item 2-5-27] Cells or cell populations described in any one of items 2-5-13 to 2-5-13AB, or differentiated cells or cell populations described in item 2-5-16, for use in regenerative medicine or in the treatment of cartilage diseases. [Item 2-5-28] Use of cells or cell populations described in any one of items 2-5-13 to 2-5-13AB, or differentiated cells or cell populations described in item 2-5-16, in the manufacture of drugs for regenerative medicine or in the treatment of cartilage diseases. [Item 2-5-29] A method for performing regenerative medicine or treating cartilage disease in a subject in need thereof, comprising the step of administering to the subject an effective amount of cells or cell populations described in any one of items 2-5-13 to 2-5-13AB, or differentiated cells or cell populations described in item 2-5-16.

[0030] Form 2-6 [Item 2-6-1] A culture medium comprising (2) and (6). [Item 2-6-2] A culture medium according to any of the above items, comprising (2') and (6'). [Item 2-6-3] A culture medium according to any one of the above items, comprising (2") and (6"). [Item 2-6-4] A culture medium according to any one of the above items, comprising (2A) and (6A). [Item 2-6-5] A culture medium according to any one of the above items, comprising (2B) and (6B). [Item 2-6-6A] A culture medium according to any one of the above items, comprising one or more of (2), (2'), (2"), (2A) and (2B), and one or more of (6), (6'), (6"), (6A) and (6B). [Item 2-6-6] A culture medium according to any one of the above items, for culturing various stem cells such as mesenchymal stem cells. [Item 2-6-6A] A culture medium according to any one of the above items, for culturing mesenchymal stem cells. [Item 2-6-6B] A serum-free culture medium according to any one of the above items. [Item 2-6-6C] A culture medium according to any one of the above items, which does not contain heterologous raw materials. [Item 2-6-7] A method for producing cells, comprising the step of culturing cells using the culture medium according to any one of the above items. [Item 2-6-8] The method according to any one of the above items, wherein the culturing step comprises culturing under conditions that satisfy at least one selected from the group consisting of a specific range of cell numbers, a specific container, and a specific temperature. [Item 2-6-8A] The range of cell numbers is such that MSCs are not seeded at low densities, preferably 500 cells / cm². ２ ~20000 cells / cm ２ That's good enough, and more preferably, 500-1000 / cm ２ ~10,000 / cm ２ For example, 2000 cells / cm² ２The method according to any one of the above items, although often seeded before or after. [Item 2-6-8B] The method according to any one of the above items, wherein the range of the cell number is adopted at the time of screening, such as 1500 cells / well, and / or carried out under conditions such as 4500 cells / well which are estimated to become confluent. [Item 2-6-8C] The method according to any one of the above items, wherein the specific container is a container of a suitable material (including with or without coating). [Item 2-6-8D] The method according to any one of the above items, wherein the specific temperature is a suitable temperature (such as 25-37°C or 30°C). [Item 2-6-9] A cell culture apparatus comprising a culture medium according to any one of the above items or means for providing the culture medium, a container capable of containing the culture medium, and means for providing conditions for culturing cells as necessary. [Item 2-6-10] A method for preserving cells, comprising the step of preserving cells using the culture medium according to any one of the above items. [Item 2-6-11] A composition for preserving cells, comprising the component described in any one of the above items. [Item 2-6-12] A cell-containing composition comprising the component described in any one of the above items and cells. [Item 2-6-13] Cells cultured using the component described in any one of the above items. [Item 2-6-13A] The cells described in any one of the above items, wherein the expression of at least one selected from the group consisting of CD44, CD73, CD90, and CD105 is not significantly reduced. [Item 2-6-13B] A cell population comprising the cells described in any of the above items, wherein the expression of at least one selected from the group consisting of CD44, CD73, CD90, and CD105 is not significantly reduced even after long-term passage. [Item 2-6-13C] The marker is at least one of CD44, CD73, CD90, and CD105, and at least one of these does not decrease. Typically, CD44 does not decrease, but the others often decrease, and it is a desirable and advantageous characteristic that CD73 and CD90, or CD44, CD90 and CD105, do not decrease, in any one of the above items, a cell or cell population.[Item 2-6-13D] Cells or cell populations according to any one of the above items, in which high expression of CD90 is maintained. [Item 2-6-13E] Cells or cell populations according to any one of the above items, in which the expression of at least one of the markers (preferably at least one of CD73, CD90, and CD105) is not significantly reduced when the number of passages is 5 or more, preferably 6 or more, and more preferably 7 or more. [Item 2-6-13F] Cells or cell populations according to any one of the above items, in which the expression of at least one of the markers (preferably at least one of CD73, CD90, and CD105) is not significantly reduced when the number of passages is 8 or more. [Item 2-6-13G] Cells or cell populations according to any one of the above items, in which differentiation ability is maintained when the number of passages is 5 or more, preferably 6 or more, and more preferably 7 or more. [Item 2-6-13H] Cells or cell populations according to any one of the above items, in which differentiation ability is maintained when the number of passages is 8 or more. [Item 2-6-13I] A cell or cell population according to any one of the above items, wherein the expression of CD90, CD44, and CD73 is maintained at 70% or more, usually 80% or more, preferably 90% or more, and more preferably 95% or more, respectively, before passage. [Item 2-6-13J] A cell or cell population according to any one of the above items, wherein the expression of CD105 is maintained at 30% or more, usually 40% or more, preferably 50% or more, and more preferably 70% or more, before passage. [Item 2-6-13AA] The cell according to item 2-6-13, wherein the cell properties are improved (for example, cartilage differentiation ability is enhanced). [Item 2-6-13AB] A cell population containing about 50% or more, about 60% or more, about 70% or more, about 80% or more, about 90% or more, or more of the cells according to any one of items 2-6-13 to 2-6-13AA. [Item 2-6-14] A pharmaceutical product comprising cells described in any one of the above items. [Item 2-6-15] A method for differentiating cells, comprising the step of culturing cells obtained using a culture medium described in any one of the above items under conditions that induce differentiation. [Item 2-6-15A] The method according to any one of the above items, wherein the culture comprises culturing the cells after proliferation using a different differentiation medium while confirming their differentiation ability.[Item 2-6-15B] The method according to any one of the above items, wherein the conditions under which the differentiation is induced also include differentiation conditions that are commonly used for MSCs. [Item 2-6-15C] The method according to any one of the above items, wherein the cells are less likely to become cartilage and / or have high cartilage differentiation potential. [Item 2-6-16] Differentiated cells obtained by differentiating stem cells by the method according to any one of the above items. [Item 2-6-17] A method for treating or preventing a subject, comprising the step of administering an effective amount of the cells or the pharmaceutical described in any one of the above items to a subject in need thereof. [Item 2-6-18] A method for quality control of stem cells, comprising determining whether they have a coefficient C correlated with the culture medium described in any one of the above items. [Item 2-6-18A] The method according to any one of the above items, wherein the coefficient C, in the case of cells, includes a coefficient relating to cell markers. [Item 2-6-18B] The method according to any one of the above items, wherein the cell marker includes common markers, such as cell markers related to proliferation. [Item 2-6-18C] The method according to any of the above items, wherein the cell marker includes markers described herein, for example, those that enhance differentiation ability or those that are expressed over a long period of time. [Item 2-6-18D] The method according to any of the above items, wherein the marker, in the case of a culture medium, includes information relating to the identification of the component itself. [Item 2-6-19] A system for culturing cells, comprising: 1) a cell culture section for containing cells intended for culture; 2) a medium supply section for providing a culture medium or raw materials according to any one of the above items; and 3) a cell culture condition adjustment section for adjusting culture conditions as necessary. [Item 2-6-20] A culture medium suitable for transporting cells containing the culture medium components described in any one of the above items. [Item 2-6-21] A method for managing cell culture for cells suitable for culturing using a culture medium described in any one of the above items, comprising: 1) providing information relating to the culture medium for cells provided by the user; 2) assigning identifiable labels to the cells and the culture medium; and 3) managing whether the cells and the culture medium are compatible by referring to the labels.

[0031] [Item 2-6-22] The use of (2) and (6) described in Item 2-6-1 for culturing cells (preferably mesenchymal stem cells). [Item 2-6-23] The use of Item 2-6-22 for culturing cells (preferably mesenchymal stem cells), comprising the features of any one of Items 2-6-2 to 2-6-6C. [Item 2-6-24] A method for maintaining or improving the quality of cells (preferably mesenchymal stem cells) (preferably, the expression level of at least one selected from the group consisting of CD44, CD73, CD90 and CD105, and / or differentiation potential after long-term passage), comprising the step of contacting the cells in a culture medium containing the components described in Item 2-6-1. [Item 2-6-25] The method according to Item 2-6-24, comprising the features of any one of Items 2-6-2 to 2-6-6C. [Item 2-6-26] Cells or cell populations described in any one of items 2-6-13 to 2-6-13AB, or differentiated cells or cell populations described in item 2-6-16, for use in treatment. [Item 2-6-27] Cells or cell populations described in any one of items 2-6-13 to 2-6-13AB, or differentiated cells or cell populations described in item 2-6-16, for use in regenerative medicine or in the treatment of cartilage diseases. [Item 2-6-28] Use of cells or cell populations described in any one of items 2-6-13 to 2-6-13AB, or differentiated cells or cell populations described in item 2-6-16, in the manufacture of drugs for regenerative medicine or in the treatment of cartilage diseases. [Item 2-6-29] A method for performing regenerative medicine or treating cartilage disease in a subject in need thereof, comprising the step of administering to the subject an effective amount of cells or cell populations described in any one of items 2-6-13 to 2-6-13AB, or differentiated cells or cell populations described in item 2-6-16.

[0032] Form 2-7 [Item 2-7-1] A culture medium comprising (2) and (7). [Item 2-7-2] A culture medium according to any of the above items, comprising (2') and (7'). [Item 2-7-3] A culture medium according to any one of the above items, comprising (2") and (7"). [Item 2-7-4] A culture medium according to any one of the above items, comprising (2A) and (7A). [Item 2-7-5] A culture medium according to any one of the above items, comprising (2B) and (7B). [Item 2-7-5A] A culture medium according to any one of the above items, comprising one or more of (2), (2'), (2"), (2A) and (2B), and one or more of (7), (7'), (7"), (7A) and (7B). [Item 2-7-6] A culture medium according to any one of the above items, for culturing various stem cells such as mesenchymal stem cells. [Item 2-7-6A] A culture medium according to any one of the above items, for culturing mesenchymal stem cells. [Item 2-7-6B] A serum-free culture medium according to any one of the above items. [Item 2-7-6C] A culture medium according to any one of the above items, which does not contain heterologous raw materials. [Item 2-7-7] A method for producing cells, comprising the step of culturing cells using the culture medium according to any one of the above items. [Item 2-7-8] The method according to any one of the above items, wherein the culturing step comprises culturing under conditions that satisfy at least one selected from the group consisting of a specific range of cell numbers, a specific container, and a specific temperature. [Item 2-7-8A] The range of cell numbers is such that MSCs are not seeded at low densities, preferably 500 cells / cm². ２ ~20000 cells / cm ２ That's good enough, and more preferably, 500-1000 / cm ２ ~10,000 / cm ２ For example, 2000 cells / cm² ２The method according to any one of the above items, although often seeded before or after. [Item 2-7-8B] The method according to any one of the above items, wherein the range of the cell number is adopted at the time of screening, such as 1500 cells / well, and / or carried out under conditions such as 4500 cells / well which are estimated to become confluent. [Item 2-7-8C] The method according to any one of the above items, wherein the specific container is a container of a suitable material (including with or without coating). [Item 2-7-8D] The method according to any one of the above items, wherein the specific temperature is a suitable temperature (such as 25-37°C or 30°C). [Item 2-7-9] A cell culture apparatus comprising a culture medium according to any one of the above items or means for providing the culture medium, a container capable of containing the culture medium, and means for providing conditions for culturing cells as necessary. [Item 2-7-10] A method for preserving cells, comprising the step of preserving cells using the culture medium according to any one of the above items. [Item 2-7-11] A composition for preserving cells, comprising the component described in any one of the above items. [Item 2-7-12] A cell-containing composition comprising the component described in any one of the above items and cells. [Item 2-7-13] Cells cultured using the component described in any one of the above items. [Item 2-7-13A] The cells described in any one of the above items, wherein the expression of at least one selected from the group consisting of CD44, CD73, CD90, and CD105 is not significantly reduced. [Item 2-7-13B] A cell population comprising the cells described in any of the above items, wherein the expression of at least one selected from the group consisting of CD44, CD73, CD90, and CD105 is not significantly reduced even after long-term passage. [Item 2-7-13C] The marker is at least one of CD44, CD73, CD90, and CD105, and at least one of these does not decrease. Typically, CD44 does not decrease, but the others often decrease, and it is a desirable and advantageous characteristic that CD73 and CD90, or CD44, CD90 and CD105, do not decrease, in any one of the above items, a cell or cell population.[Item 2-7-13D] Cells or cell populations according to any one of the above items, in which high expression of CD90 is maintained. [Item 2-7-13E] Cells or cell populations according to any one of the above items, in which the expression of at least one of the markers (preferably at least one of CD73, CD90, and CD105) is not significantly reduced when the number of passages is 5 or more, preferably 6 or more, and more preferably 7 or more. [Item 2-7-13F] Cells or cell populations according to any one of the above items, in which the expression of at least one of the markers (preferably at least one of CD73, CD90, and CD105) is not significantly reduced when the number of passages is 8 or more. [Item 2-7-13G] Cells or cell populations according to any one of the above items, in which differentiation ability is maintained when the number of passages is 5 or more, preferably 6 or more, and more preferably 7 or more. [Item 2-7-13H] Cells or cell populations according to any one of the above items, in which differentiation ability is maintained when the number of passages is 8 or more. [Item 2-7-13I] A cell or cell population according to any one of the above items, wherein the expression of CD90, CD44, and CD73 is maintained at 70% or more, usually 80% or more, preferably 90% or more, and more preferably 95% or more, respectively, before passage. [Item 2-7-13J] A cell or cell population according to any one of the above items, wherein the expression of CD105 is maintained at 30% or more, usually 40% or more, preferably 50% or more, and more preferably 70% or more, before passage. [Item 2-7-13AA] The cell according to item 2-7-13, wherein the properties of the cell are improved (for example, the ability to differentiate into cartilage is enhanced). [Item 2-7-13AB] A cell population containing about 50% or more, about 60% or more, about 70% or more, about 80% or more, about 90% or more, or more, of the cells according to any one of items 2-7-13 to 2-7-13AA. [Item 2-7-14] A pharmaceutical product comprising cells described in any one of the above items. [Item 2-7-15] A method for differentiating cells, comprising the step of culturing cells obtained using a culture medium described in any one of the above items under conditions that induce differentiation. [Item 2-7-15A] The method according to any one of the above items, wherein the culture comprises culturing the cells after proliferation using a different differentiation medium while confirming their differentiation ability.[Item 2-7-15B] The method according to any one of the above items, wherein the conditions under which the differentiation is induced also include differentiation conditions that are commonly used for MSCs. [Item 2-7-15C] The method according to any one of the above items, wherein the cells are less likely to become cartilage and / or have high cartilage differentiation potential. [Item 2-7-16] Differentiated cells obtained by differentiating stem cells by the method according to any one of the above items. [Item 2-7-17] A method for treating or preventing a subject, comprising the step of administering an effective amount of the cells or the pharmaceutical described in any one of the above items to a subject in need thereof. [Item 2-7-18] A method for quality control of stem cells, comprising determining whether they have a coefficient C correlated with the culture medium described in any one of the above items. [Item 2-7-18A] The method according to any one of the above items, wherein the coefficient C, in the case of cells, includes a coefficient relating to cell markers. [Item 2-7-18B] The method according to any one of the above items, wherein the cell marker includes common markers, such as cell markers related to proliferation. [Item 2-7-18C] The method according to any of the above items, wherein the cell marker includes markers described herein, for example, those that enhance differentiation ability or those that are expressed over a long period of time. [Item 2-7-18D] The method according to any of the above items, wherein the marker, in the case of a culture medium, includes information relating to the identification of the component itself. [Item 2-7-19] A system for culturing cells, comprising: 1) a cell culture section for containing cells intended for culture; 2) a medium supply section for providing a culture medium or raw materials according to any one of the above items; and 3) a cell culture condition adjustment section for adjusting culture conditions as necessary. [Item 2-7-20] A culture medium suitable for transporting cells containing culture medium components according to any one of the above items. [Item 2-7-21] A method for managing cell culture for cells suitable for culturing using a culture medium according to any one of the above items, comprising: 1) providing information relating to the culture medium for cells provided by the user; 2) assigning identifiable labels to the cells and the culture medium; and 3) managing whether the cells and the culture medium are compatible by referring to the labels.

[0033] [Item 2-7-22] The use of (2) and (7) of Item 2-7-1 for culturing cells (preferably mesenchymal stem cells). [Item 2-7-23] The use of Item 2-7-22 for culturing cells (preferably mesenchymal stem cells), comprising the features of any one of Items 2-7-2 to 2-7-6C. [Item 2-7-24] A method for maintaining or improving the quality of cells (preferably mesenchymal stem cells) (preferably, the expression level of at least one selected from the group consisting of CD44, CD73, CD90 and CD105, and / or differentiation potential after long-term passage), comprising the step of contacting the cells in a culture medium containing the components of Item 2-7-1. [Item 2-7-25] The method of Item 2-7-24, comprising the features of any one of Items 2-7-2 to 2-7-6C. [Item 2-7-26] Cells or cell populations described in any one of items 2-7-13 to 2-7-13AB, or differentiated cells or cell populations described in item 2-7-16, for use in treatment. [Item 2-7-27] Cells or cell populations described in any one of items 2-7-13 to 2-7-13AB, or differentiated cells or cell populations described in item 2-7-16, for use in regenerative medicine or in the treatment of cartilage diseases. [Item 2-7-28] Use of cells or cell populations described in any one of items 2-7-13 to 2-7-13AB, or differentiated cells or cell populations described in item 2-7-16, in the manufacture of drugs for regenerative medicine or in the treatment of cartilage diseases. [Item 2-7-29] A method for performing regenerative medicine or treating cartilage disease in a subject in need thereof, comprising the step of administering to the subject an effective amount of cells or cell populations described in any one of items 2-7-13 to 2-7-13AB, or differentiated cells or cell populations described in item 2-7-16.

[0034] Form 3-4 [Item 3-4-1] A culture medium comprising (3) and (4). [Item 3-4-2] A culture medium according to any of the above items, comprising (3') and (4'). [Item 3-4-3] A culture medium according to any one of the above items, comprising (3") and (4"). [Item 3-4-4] A culture medium according to any one of the above items, comprising (3A) and (4A). [Item 3-4-5] A culture medium according to any one of the above items, comprising (3B) and (4B). [Item 3-4-5A] A culture medium according to any one of the above items, comprising one or more of (3), (3'), (3"), (3A) and (3B), and one or more of (4), (4'), (4"), (4A) and (4B). [Item 3-4-6] A culture medium according to any one of the above items, for culturing various stem cells such as mesenchymal stem cells. [Item 3-4-6A] A culture medium according to any one of the above items, for culturing mesenchymal stem cells. [Item 3-4-6B] A serum-free culture medium according to any one of the above items. [Item 3-4-6C] A culture medium according to any one of the above items, which does not contain heterologous raw materials. [Item 3-4-7] A method for producing cells, comprising the step of culturing cells using the culture medium according to any one of the above items. [Item 3-4-8] The method according to any one of the above items, wherein the culturing step comprises culturing under conditions that satisfy at least one selected from the group consisting of a specific range of cell numbers, a specific container, and a specific temperature. [Item 3-4-8A] The range of cell numbers is such that MSCs are not seeded at low densities, preferably 500 cells / cm². ２ ~20000 cells / cm ２ That's good enough, and more preferably, 500-1000 / cm ２ ~10,000 / cm ２ For example, 2000 cells / cm² ２The method according to any one of the above items, although often seeded before or after. [Item 3-4-8B] The method according to any one of the above items, wherein the range of the cell number is a specific value, such as 1500 cells / well, at the time of screening, and / or is carried out under conditions such as 4500 cells / well, which are estimated to be confluent. [Item 3-4-8C] The method according to any one of the above items, wherein the specific container is a container of a suitable material (including with or without coating). [Item 3-4-8D] The method according to any one of the above items, wherein the specific temperature is a suitable temperature (such as 25-37°C or 30°C). [Item 3-4-9] A cell culture apparatus comprising a culture medium according to any one of the above items or means for providing the culture medium, a container capable of containing the culture medium, and means for providing conditions for culturing cells as necessary. [Item 3-4-10] A method for preserving cells, comprising the step of preserving cells using the culture medium according to any one of the above items. [Item 3-4-11] A composition for preserving cells, comprising the component described in any one of the above items. [Item 3-4-12] A cell-containing composition comprising the component described in any one of the above items and cells. [Item 3-4-13] Cells cultured using the component described in any one of the above items. [Item 3-4-13A] The cells described in any one of the above items, wherein the expression of at least one selected from the group consisting of CD44, CD73, CD90, and CD105 is not significantly reduced. [Item 3-4-13B] A cell population comprising the cells described in any of the above items, wherein the expression of at least one selected from the group consisting of CD44, CD73, CD90, and CD105 is not significantly reduced even after long-term passage. [Item 3-4-13C] The marker is at least one of CD44, CD73, CD90, and CD105, and at least one of these does not decrease. Typically, CD44 does not decrease, but the others often decrease, and it is a desirable and advantageous characteristic that CD73 and CD90, or CD44, CD90 and CD105, do not decrease, in any one of the above items, a cell or cell population.[Item 3-4-13D] Cells or cell populations according to any one of the above items, in which high expression of CD90 is maintained. [Item 3-4-13E] Cells or cell populations according to any one of the above items, in which the expression of at least one of the markers (preferably at least one of CD73, CD90, and CD105) is not significantly reduced when the number of passages is 5 or more, preferably 6 or more, and more preferably 7 or more. [Item 3-4-13F] Cells or cell populations according to any one of the above items, in which the expression of at least one of the markers (preferably at least one of CD73, CD90, and CD105) is not significantly reduced when the number of passages is 8 or more. [Item 3-4-13G] Cells or cell populations according to any one of the above items, in which differentiation ability is maintained when the number of passages is 5 or more, preferably 6 or more, and more preferably 7 or more. [Item 3-4-13H] Cells or cell populations according to any one of the above items, in which differentiation ability is maintained when the number of passages is 8 or more. [Item 3-4-13I] A cell or cell population according to any one of the above items, wherein the expression of CD90, CD44, and CD73 is maintained at 70% or more, usually 80% or more, preferably 90% or more, and more preferably 95% or more, respectively, before passage. [Item 3-4-13J] A cell or cell population according to any one of the above items, wherein the expression of CD105 is maintained at 30% or more, usually 40% or more, preferably 50% or more, and more preferably 70% or more, before passage. [Item 3-4-13AA] The cell according to item 3-4-13, wherein the properties of the cell are improved (for example, the ability to differentiate into cartilage is enhanced). [Item 3-4-13AB] A cell population containing about 50% or more, about 60% or more, about 70% or more, about 80% or more, about 90% or more, or more, of the cells according to any one of items 3-4-13 to 3-4-13AA. [Item 3-4-14] A pharmaceutical product comprising cells described in any one of the above items. [Item 3-4-15] A method for differentiating cells, comprising the step of culturing cells obtained using a culture medium described in any one of the above items under conditions that induce differentiation. [Item 3-4-15A] The method according to any one of the above items, wherein the culture comprises culturing the cells after proliferation using a different differentiation medium while confirming the differentiation ability.[Item 3-4-15B] The method according to any one of the above items, wherein the conditions under which the differentiation is induced also include differentiation conditions that are commonly used for MSCs. [Item 3-4-15C] The method according to any one of the above items, wherein the cells are less likely to become cartilage and / or have high cartilage differentiation potential. [Item 3-4-16] Differentiated cells obtained by differentiating stem cells by the method according to any one of the above items. [Item 3-4-17] A method for treating or preventing a subject, comprising the step of administering an effective amount of the cells or the pharmaceutical described in any one of the above items to a subject in need thereof. [Item 3-4-18] A method for quality control of stem cells, comprising determining whether they have a coefficient C correlated with the culture medium described in any one of the above items. [Item 3-4-18A] The method according to any one of the above items, wherein the coefficient C, in the case of cells, includes a coefficient relating to cell markers. [Item 3-4-18B] The method according to any one of the above items, wherein the cell marker includes common markers, such as cell markers related to proliferation. [Item 3-4-18C] The method according to any of the above items, wherein the cell marker includes markers described herein, for example, those that enhance differentiation ability or those that are expressed over a long period of time. [Item 3-4-18D] The method according to any of the above items, wherein the marker, in the case of a culture medium, includes information relating to the identification of the component itself. [Item 3-4-19] A system for culturing cells, comprising: 1) a cell culture section for containing cells intended for culture; 2) a medium supply section for providing a culture medium or raw materials according to any of the above items; and 3) a cell culture condition adjustment section for adjusting culture conditions as necessary. [Item 3-4-20] A culture medium suitable for transporting cells containing the culture medium components according to any of the above items. [Item 3-4-21] A method for managing cell culture for cells suitable for culturing using the culture medium according to any of the above items, comprising: 1) providing information relating to the culture medium for cells provided by the user; 2) assigning identifiable labels to the cells and the culture medium; and 3) managing whether the cells and the culture medium are compatible by referring to the labels.

[0035] [Item 3-4-22] The use of (3) and (4) of Item 3-4-1 for culturing cells (preferably mesenchymal stem cells). [Item 3-4-23] The use of Item 3-4-22 for culturing cells (preferably mesenchymal stem cells), comprising the features of any one of Items 3-4-2 to 3-4-6C. [Item 3-4-24] A method for maintaining or improving the quality of cells (preferably mesenchymal stem cells) (preferably, the expression level of at least one selected from the group consisting of CD44, CD73, CD90 and CD105, and / or differentiation potential after long-term passage), comprising the step of contacting the cells in a culture medium containing the components of Item 3-4-1. [Item 3-4-25] The method of Item 3-4-24, comprising the features of any one of Items 3-4-2 to 3-4-6C. [Item 3-4-26] Cells or cell populations described in any one of items 3-4-13 to 3-4-13AB, or differentiated cells or cell populations described in item 3-4-16, for use in treatment. [Item 3-4-27] Cells or cell populations described in any one of items 3-4-13 to 3-4-13AB, or differentiated cells or cell populations described in item 3-4-16, for use in regenerative medicine or in the treatment of cartilage diseases. [Item 3-4-28] Use of cells or cell populations described in any one of items 3-4-13 to 3-4-13AB, or differentiated cells or cell populations described in item 3-4-16, in the manufacture of drugs for regenerative medicine or in the treatment of cartilage diseases. [Item 3-4-29] A method for performing regenerative medicine or treating cartilage disease in a subject in need thereof, comprising the step of administering to the subject an effective amount of cells or cell populations described in any one of items 3-4-13 to 3-4-13AB, or differentiated cells or cell populations described in item 3-4-16.

[0036] Form 3-5 [Item 3-5-1] A culture medium comprising (3) and (5). [Item 3-5-2] A culture medium according to any of the above items, comprising (3') and (5'). [Item 3-5-3] A culture medium according to any one of the above items, comprising (3") and (5"). [Item 3-5-4] A culture medium according to any one of the above items, comprising (3A) and (5A). [Item 3-5-5] A culture medium according to any one of the above items, comprising (3B) and (5B). [Item 3-5-5A] A culture medium according to any one of the above items, comprising one or more of (3), (3'), (3"), (3A) and (3B), and one or more of (5), (5'), (5"), (5A) and (5B). [Item 3-5-6] A culture medium according to any one of the above items, for culturing various stem cells such as mesenchymal stem cells. [Item 3-5-6A] A culture medium according to any one of the above items, for culturing mesenchymal stem cells. [Item 3-5-6B] A serum-free culture medium according to any one of the above items. [Item 3-5-6C] A culture medium according to any one of the above items, which does not contain heterologous raw materials. [Item 3-5-7] A method for producing cells, comprising the step of culturing cells using the culture medium according to any one of the above items. [Item 3-5-8] The method according to any one of the above items, wherein the culturing step comprises culturing under conditions that satisfy at least one selected from the group consisting of a specific range of cell numbers, a specific container, and a specific temperature. [Item 3-5-8A] The range of cell numbers is such that MSCs are not seeded at low densities, preferably 500 cells / cm². ２ ~20000 cells / cm ２ That's good enough, and more preferably, 500-1000 / cm ２ ~10,000 / cm ２ For example, 2000 cells / cm² ２The method according to any one of the above items, although often seeded before or after. [Item 3-5-8B] The method according to any one of the above items, wherein the range of the cell number is a specific value, such as 1500 cells / well, at the time of screening, and / or is carried out under conditions such as 4500 cells / well, which are estimated to become confluent. [Item 3-5-8C] The method according to any one of the above items, wherein the specific container is a container of a suitable material (including with or without coating). [Item 3-5-8D] The method according to any one of the above items, wherein the specific temperature is a suitable temperature (such as 25-37°C or 30°C). [Item 3-5-9] A cell culture apparatus comprising a culture medium according to any one of the above items or means for providing the culture medium, a container capable of containing the culture medium, and means for providing conditions for culturing cells as necessary. [Item 3-5-10] A method for preserving cells, comprising the step of preserving cells using the culture medium according to any one of the above items. [Item 3-5-11] A composition for preserving cells, comprising the component described in any one of the above items. [Item 3-5-12] A cell-containing composition comprising the component described in any one of the above items and cells. [Item 3-5-13] Cells cultured using the component described in any one of the above items. [Item 3-5-13A] The cells described in any one of the above items, wherein the expression of at least one selected from the group consisting of CD44, CD73, CD90, and CD105 is not significantly reduced. [Item 3-5-13B] A cell population comprising the cells described in any of the above items, wherein the expression of at least one selected from the group consisting of CD44, CD73, CD90, and CD105 is not significantly reduced even after long-term passage. [Item 3-5-13C] The marker is at least one of CD44, CD73, CD90, and CD105, and at least one of these does not decrease. Typically, CD44 does not decrease, but the others often decrease, and it is a desirable and advantageous characteristic that CD73 and CD90, or CD44, CD90 and CD105, do not decrease, in any one of the above items, a cell or cell population.[Item 3-5-13D] Cells or cell populations according to any one of the above items, in which high expression of CD90 is maintained. [Item 3-5-13E] Cells or cell populations according to any one of the above items, in which the expression of at least one of the markers (preferably at least one of CD73, CD90, and CD105) is not significantly reduced when the number of passages is 5 or more, preferably 6 or more, and more preferably 7 or more. [Item 3-5-13F] Cells or cell populations according to any one of the above items, in which the expression of at least one of the markers (preferably at least one of CD73, CD90, and CD105) is not significantly reduced when the number of passages is 8 or more. [Item 3-5-13G] Cells or cell populations according to any one of the above items, in which differentiation ability is maintained when the number of passages is 5 or more, preferably 6 or more, and more preferably 7 or more. [Item 3-5-13H] Cells or cell populations according to any one of the above items, in which differentiation ability is maintained when the number of passages is 8 or more. [Item 3-5-13I] A cell or cell population according to any one of the above items, wherein the expression of CD90, CD44, and CD73 is maintained at 70% or more, usually 80% or more, preferably 90% or more, and more preferably 95% or more, respectively, before passage. [Item 3-5-13J] A cell or cell population according to any one of the above items, wherein the expression of CD105 is maintained at 30% or more, usually 40% or more, preferably 50% or more, and more preferably 70% or more, before passage. [Item 3-5-13AA] The cell according to item 3-5-13, wherein the cell properties are improved (for example, cartilage differentiation ability is enhanced). [Item 3-5-13AB] A cell population containing about 50% or more, about 60% or more, about 70% or more, about 80% or more, about 90% or more, or more of the cells according to any one of items 3-5-13 to 3-5-13AA. [Item 3-5-14] A pharmaceutical product comprising cells described in any one of the above items. [Item 3-5-15] A method for differentiating cells, comprising the step of culturing cells obtained using a culture medium described in any one of the above items under conditions that induce differentiation. [Item 3-5-15A] The method according to any one of the above items, wherein the culture comprises culturing the cells after proliferation using a different differentiation medium while confirming the differentiation ability.[Item 3-5-15B] The method according to any one of the above items, wherein the conditions under which the differentiation is induced also include differentiation conditions that are commonly used for MSCs. [Item 3-5-15C] The method according to any one of the above items, wherein the cells are less likely to become cartilage and / or have high cartilage differentiation potential. [Item 3-5-16] Differentiated cells obtained by differentiating stem cells by the method according to any one of the above items. [Item 3-5-17] A method for treating or preventing a subject, comprising the step of administering an effective amount of the cells or the pharmaceutical described in any one of the above items to a subject in need thereof. [Item 3-5-18] A method for quality control of stem cells, comprising determining whether they have a coefficient C correlated with the culture medium described in any one of the above items. [Item 3-5-18A] The method according to any one of the above items, wherein the coefficient C, in the case of cells, includes a coefficient relating to cell markers. [Item 3-5-18B] The method according to any one of the above items, wherein the cell marker includes common markers, such as cell markers associated with proliferation. [Item 3-5-18C] The method according to any of the above items, wherein the cell marker includes markers described herein, for example, those that enhance differentiation ability or those that are expressed over a long period of time. [Item 3-5-18D] The method according to any of the above items, wherein the marker, in the case of a culture medium, includes information relating to the identification of the component itself. [Item 3-5-19] A system for culturing cells, comprising: 1) a cell culture section for containing cells intended for culture; 2) a culture medium supply section for providing a culture medium or raw materials according to any of the above items; and 3) a cell culture condition adjustment section for adjusting culture conditions as necessary. [Item 3-5-20] A culture medium suitable for transporting cells containing culture medium components according to any of the above items. [Item 3-5-21] A method for managing cell culture for cells suitable for culturing using a culture medium according to any of the above items, comprising: 1) providing information relating to the culture medium for cells provided by the user; 2) assigning identifiable labels to the cells and the culture medium; and 3) managing whether the cells and the culture medium are compatible by referring to the labels.

[0037] [Item 3-5-22] The use of (3) and (5) of Item 3-5-1 for culturing cells (preferably mesenchymal stem cells). [Item 3-5-23] The use of Item 3-5-22 for culturing cells (preferably mesenchymal stem cells), comprising the features of any one of Items 3-5-2 to 3-5-6C. [Item 3-5-24] A method for maintaining or improving the quality of cells (preferably mesenchymal stem cells) (preferably, the expression level of at least one selected from the group consisting of CD44, CD73, CD90 and CD105, and / or differentiation potential after long-term passage), comprising the step of contacting the cells in a culture medium containing the components of Item 3-5-1. [Item 3-5-25] The method of Item 3-5-24, comprising the features of any one of Items 3-5-2 to 3-5-6C. [Item 3-5-26] Cells or cell populations described in any one of items 3-5-13 to 3-5-13AB, or differentiated cells or cell populations described in item 3-5-16, for use in treatment. [Item 3-5-27] Cells or cell populations described in any one of items 3-5-13 to 3-5-13AB, or differentiated cells or cell populations described in item 3-5-16, for use in regenerative medicine or in the treatment of cartilage diseases. [Item 3-5-28] Use of cells or cell populations described in any one of items 3-5-13 to 3-5-13AB, or differentiated cells or cell populations described in item 3-5-16, in the manufacture of drugs for regenerative medicine or in the treatment of cartilage diseases. [Item 3-5-29] A method for performing regenerative medicine or treating cartilage disease in a subject in need thereof, comprising the step of administering to the subject an effective amount of cells or cell populations described in any one of items 3-5-13 to 3-5-13AB, or differentiated cells or cell populations described in item 3-5-16.

[0038] Form 3-6 [Item 3-6-1] A culture medium comprising (3) and (6). [Item 3-6-2] A culture medium according to any of the above items, comprising (3') and (6'). [Item 3-6-3] A culture medium according to any one of the above items, comprising (3") and (6"). [Item 3-6-4] A culture medium according to any one of the above items, comprising (3A) and (6A). [Item 3-6-5] A culture medium according to any one of the above items, comprising (3B) and (6B). [Item 3-6-6A] A culture medium according to any one of the above items, comprising one or more of (3), (3'), (3"), (3A) and (3B), and one or more of (6), (6'), (6"), (6A) and (6B). [Item 3-6-6] A culture medium according to any one of the above items, for culturing various stem cells such as mesenchymal stem cells. [Item 3-6-6A] A culture medium according to any one of the above items, for culturing mesenchymal stem cells. [Item 3-6-6B] A serum-free culture medium according to any one of the above items. [Item 3-6-6C] A culture medium according to any one of the above items, which does not contain heterologous raw materials. [Item 3-6-7] A method for producing cells, comprising the step of culturing cells using the culture medium according to any one of the above items. [Item 3-6-8] The method according to any one of the above items, wherein the culturing step comprises culturing under conditions that satisfy at least one selected from the group consisting of a specific range of cell numbers, a specific container, and a specific temperature. [Item 3-6-8A] The range of cell numbers is such that MSCs are not seeded at low densities, preferably 500 cells / cm². ２ ~20000 cells / cm ２ That's good enough, and more preferably, 500-1000 / cm ２ ~10,000 / cm ２ For example, 2000 cells / cm² ２The method according to any one of the above items, although often seeded before or after. [Item 3-6-8B] The method according to any one of the above items, wherein the range of the cell number is a specific value, such as 1500 cells / well, at the time of screening, and / or is carried out under conditions such as 4500 cells / well, which are estimated to be confluent. [Item 3-6-8C] The method according to any one of the above items, wherein the specific container is a container of a suitable material (including with or without coating). [Item 3-6-8D] The method according to any one of the above items, wherein the specific temperature is a suitable temperature (such as 25-37°C or 30°C). [Item 3-6-9] A cell culture apparatus comprising a culture medium according to any one of the above items or means for providing the culture medium, a container capable of containing the culture medium, and means for providing conditions for culturing cells as necessary. [Item 3-6-10] A method for preserving cells, comprising the step of preserving cells using the culture medium according to any one of the above items. [Item 3-6-11] A composition for preserving cells, comprising the component described in any one of the above items. [Item 3-6-12] A cell-containing composition comprising the component described in any one of the above items and cells. [Item 3-6-13] Cells cultured using the component described in any one of the above items. [Item 3-6-13A] The cells described in any one of the above items, wherein the expression of at least one selected from the group consisting of CD44, CD73, CD90, and CD105 is not significantly reduced. [Item 3-6-13B] A cell population comprising the cells described in any of the above items, wherein the expression of at least one selected from the group consisting of CD44, CD73, CD90, and CD105 is not significantly reduced even after long-term passage. [Item 3-6-13C] The marker is at least one of CD44, CD73, CD90, and CD105, and at least one of these does not decrease. Typically, CD44 does not decrease, but the others often decrease, and it is a desirable and advantageous characteristic that CD73 and CD90, or CD44, CD90 and CD105, do not decrease, in any one of the above items, a cell or cell population.[Item 3-6-13D] Cells or cell populations according to any one of the above items, in which high expression of CD90 is maintained. [Item 3-6-13E] Cells or cell populations according to any one of the above items, in which the expression of at least one of the markers (preferably at least one of CD73, CD90, and CD105) is not significantly reduced when the number of passages is 5 or more, preferably 6 or more, and more preferably 7 or more. [Item 3-6-13F] Cells or cell populations according to any one of the above items, in which the expression of at least one of the markers (preferably at least one of CD73, CD90, and CD105) is not significantly reduced when the number of passages is 8 or more. [Item 3-6-13G] Cells or cell populations according to any one of the above items, in which differentiation ability is maintained when the number of passages is 5 or more, preferably 6 or more, and more preferably 7 or more. [Item 3-6-13H] Cells or cell populations according to any one of the above items, in which differentiation ability is maintained when the number of passages is 8 or more. [Item 3-6-13I] A cell or cell population according to any one of the above items, wherein the expression of CD90, CD44, and CD73 is maintained at 70% or more, usually 80% or more, preferably 90% or more, and more preferably 95% or more, respectively, before passage. [Item 3-6-13J] A cell or cell population according to any one of the above items, wherein the expression of CD105 is maintained at 30% or more, usually 40% or more, preferably 50% or more, and more preferably 70% or more, before passage. [Item 3-6-13AA] The cell according to item 3-6-13, wherein the properties of the cell are improved (for example, the ability to differentiate into cartilage is enhanced). [Item 3-6-13AB] A cell population containing about 50% or more, about 60% or more, about 70% or more, about 80% or more, about 90% or more, or more of the cells according to any one of items 3-6-13 to 3-6-13AA. [Item 3-6-14] A pharmaceutical product comprising cells described in any one of the above items. [Item 3-6-15] A method for differentiating cells, comprising the step of culturing cells obtained using a culture medium described in any one of the above items under conditions that induce differentiation. [Item 3-6-15A] The method according to any one of the above items, wherein the culture comprises culturing the cells after proliferation using a different differentiation medium while confirming their differentiation ability.[Item 3-6-15B] The method according to any one of the above items, wherein the conditions under which the differentiation is induced also include differentiation conditions that are commonly used for MSCs. [Item 3-6-15C] The method according to any one of the above items, wherein the cells are less likely to become cartilage and / or have high cartilage differentiation potential. [Item 3-6-16] Differentiated cells obtained by differentiating stem cells by the method according to any one of the above items. [Item 3-6-17] A method for treating or preventing a subject, comprising the step of administering an effective amount of the cells or the pharmaceutical described in any one of the above items to a subject in need thereof. [Item 3-6-18] A method for quality control of stem cells, comprising determining whether they have a coefficient C correlated with the culture medium described in any one of the above items. [Item 3-6-18A] The method according to any one of the above items, wherein the coefficient C, in the case of cells, includes a coefficient relating to cell markers. [Item 3-6-18B] The method according to any one of the above items, wherein the cell marker includes common markers, such as cell markers associated with proliferation. [Item 3-6-18C] The method according to any of the above items, wherein the cell marker includes markers described herein, for example, those that enhance differentiation ability or those that are expressed over a long period of time. [Item 3-6-18D] The method according to any of the above items, wherein the marker, in the case of a culture medium, includes information relating to the identification of the component itself. [Item 3-6-19] A system for culturing cells, comprising: 1) a cell culture section for containing cells intended for culture; 2) a culture medium supply section for providing a culture medium or raw materials according to any of the above items; and 3) a cell culture condition adjustment section for adjusting culture conditions as necessary. [Item 3-6-20] A culture medium suitable for transporting cells containing culture medium components according to any of the above items. [Item 3-6-21] A method for managing cell culture for cells suitable for culturing using a culture medium according to any of the above items, comprising: 1) providing information relating to the culture medium for cells provided by the user; 2) assigning identifiable labels to the cells and the culture medium; and 3) managing whether the cells and the culture medium are compatible by referring to the labels.

[0039] [Item 3-6-22] The use of (3) and (6) described in Item 3-6-1 for culturing cells (preferably mesenchymal stem cells). [Item 3-6-23] The use of Item 3-6-22 for culturing cells (preferably mesenchymal stem cells), comprising the features of any one of Items 3-6-2 to 3-6-6C. [Item 3-6-24] A method for maintaining or improving the quality of cells (preferably mesenchymal stem cells) (preferably, the expression level of at least one selected from the group consisting of CD44, CD73, CD90 and CD105, and / or differentiation potential after long-term passage), comprising the step of contacting the cells in a culture medium containing the components described in Item 3-6-1. [Item 3-6-25] The method according to Item 3-6-24, comprising the features of any one of Items 3-6-2 to 3-6-6C. [Item 3-6-26] Cells or cell populations described in any one of items 3-6-13 to 3-6-13AB, or differentiated cells or cell populations described in item 3-6-16, for use in treatment. [Item 3-6-27] Cells or cell populations described in any one of items 3-6-13 to 3-6-13AB, or differentiated cells or cell populations described in item 3-6-16, for use in regenerative medicine or in the treatment of cartilage diseases. [Item 3-6-28] Use of cells or cell populations described in any one of items 3-6-13 to 3-6-13AB, or differentiated cells or cell populations described in item 3-6-16, in the manufacture of drugs for regenerative medicine or in the treatment of cartilage diseases. [Item 3-6-29] A method for performing regenerative medicine or treating cartilage disease in a subject in need thereof, comprising the step of administering to the subject an effective amount of cells or cell populations described in any one of items 3-6-13 to 3-6-13AB, or differentiated cells or cell populations described in item 3-6-16.

[0040] Form 3-7 [Item 3-7-1] A culture medium comprising (2) and (7). [Item 3-7-2] A culture medium according to any of the above items, comprising (3') and (7'). [Item 3-7-3] A culture medium according to any one of the above items, comprising (3") and (7"). [Item 3-7-4] A culture medium according to any one of the above items, comprising (3A) and (7A). [Item 3-7-5] A culture medium according to any one of the above items, comprising (3B) and (7B). [Item 3-7-5A] A culture medium according to any one of the above items, comprising one or more of (3), (3'), (3"), (3A) and (3B), and one or more of (7), (7'), (7"), (7A) and (7B). [Item 3-7-6] A culture medium according to any one of the above items, for culturing various stem cells such as mesenchymal stem cells. [Item 3-7-6A] A culture medium according to any one of the above items, for culturing mesenchymal stem cells. [Item 3-7-6B] A serum-free culture medium according to any one of the above items. [Item 3-7-6C] A culture medium according to any one of the above items, which does not contain heterologous raw materials. [Item 3-7-7] A method for producing cells, comprising the step of culturing cells using the culture medium according to any one of the above items. [Item 3-7-8] The method according to any one of the above items, wherein the culturing step comprises culturing under conditions that satisfy at least one selected from the group consisting of a specific range of cell numbers, a specific container, and a specific temperature. [Item 3-7-8A] The range of cell numbers is such that MSCs are not seeded at low densities, preferably 500 cells / cm². ２ ~20000 cells / cm ２ That's good enough, and more preferably, 500-1000 / cm ２ ~10,000 / cm ２ For example, 2000 cells / cm² ２The method according to any one of the above items, although often seeded before or after. [Item 3-7-8B] The method according to any one of the above items, wherein the range of the cell number is a specific value, such as 1500 cells / well, at the time of screening, and / or is carried out under conditions such as 4500 cells / well, which are estimated to be confluent. [Item 3-7-8C] The method according to any one of the above items, wherein the specific container is a container of a suitable material (including with or without coating). [Item 3-7-8D] The method according to any one of the above items, wherein the specific temperature is a suitable temperature (such as 25-37°C or 30°C). [Item 3-7-9] A cell culture apparatus comprising a culture medium according to any one of the above items or means for providing the culture medium, a container capable of containing the culture medium, and means for providing conditions for culturing cells as necessary. [Item 3-7-10] A method for preserving cells, comprising the step of preserving cells using the culture medium according to any one of the above items. [Item 3-7-11] A composition for preserving cells, comprising the component described in any one of the above items. [Item 3-7-12] A cell-containing composition comprising the component described in any one of the above items and cells. [Item 3-7-13] Cells cultured using the component described in any one of the above items. [Item 3-7-13A] The cells described in any one of the above items, wherein the expression of at least one selected from the group consisting of CD44, CD73, CD90, and CD105 is not significantly reduced. [Item 3-7-13B] A cell population comprising the cells described in any of the above items, wherein the expression of at least one selected from the group consisting of CD44, CD73, CD90, and CD105 is not significantly reduced even after long-term passage. [Item 3-7-13C] The marker is at least one of CD44, CD73, CD90, and CD105, and at least one of these does not decrease. Typically, CD44 does not decrease, but the others often decrease, and it is a desirable and advantageous characteristic that CD73 and CD90, or CD44, CD90 and CD105, do not decrease, in any one of the above items, a cell or cell population.[Item 3-7-13D] Cells or cell populations according to any one of the above items, in which high expression of CD90 is maintained. [Item 3-7-13E] Cells or cell populations according to any one of the above items, in which the expression of at least one of the markers (preferably at least one of CD73, CD90, and CD105) is not significantly reduced when the number of passages is 5 or more, preferably 6 or more, and more preferably 7 or more. [Item 3-7-13F] Cells or cell populations according to any one of the above items, in which the expression of at least one of the markers (preferably at least one of CD73, CD90, and CD105) is not significantly reduced when the number of passages is 8 or more. [Item 3-7-13G] Cells or cell populations according to any one of the above items, in which differentiation ability is maintained when the number of passages is 5 or more, preferably 6 or more, and more preferably 7 or more. [Item 3-7-13H] Cells or cell populations according to any one of the above items, in which differentiation ability is maintained when the number of passages is 8 or more. [Item 3-7-13I] A cell or cell population according to any one of the above items, wherein the expression of CD90, CD44, and CD73 is maintained at 70% or more, usually 80% or more, preferably 90% or more, and more preferably 95% or more, respectively, before passage. [Item 3-7-13J] A cell or cell population according to any one of the above items, wherein the expression of CD105 is maintained at 30% or more, usually 40% or more, preferably 50% or more, and more preferably 70% or more, before passage. [Item 3-7-13AA] The cell according to item 3-7-13, wherein the cell properties are improved (for example, cartilage differentiation ability is enhanced). [Item 3-7-13AB] A cell population containing about 50% or more, about 60% or more, about 70% or more, about 80% or more, about 90% or more, or more of the cells according to any one of items 3-7-13 to 3-7-13AA. [Item 3-7-14] A pharmaceutical product comprising cells described in any one of the above items. [Item 3-7-15] A method for differentiating cells, comprising the step of culturing cells obtained using a culture medium described in any one of the above items under conditions that induce differentiation. [Item 3-7-15A] The method according to any one of the above items, wherein the culture comprises culturing the cells after proliferation using a different differentiation medium while confirming their differentiation ability.[Item 3-7-15B] The method according to any one of the above items, wherein the conditions under which the differentiation is induced also include differentiation conditions that are commonly used for MSCs. [Item 3-7-15C] The method according to any one of the above items, wherein the cells are less likely to become cartilage and / or have high cartilage differentiation potential. [Item 3-7-16] Differentiated cells obtained by differentiating stem cells by the method according to any one of the above items. [Item 3-7-17] A method for treating or preventing a subject, comprising the step of administering an effective amount of the cells or the pharmaceutical described in any one of the above items to a subject in need thereof. [Item 3-7-18] A method for quality control of stem cells, comprising determining whether they have a coefficient C correlated with the culture medium described in any one of the above items. [Item 3-7-18A] The method according to any one of the above items, wherein the coefficient C, in the case of cells, includes a coefficient relating to cell markers. [Item 3-7-18B] The method according to any one of the above items, wherein the cell marker includes common markers, such as cell markers related to proliferation. [Item 3-7-18C] The method according to any of the above items, wherein the cell marker includes markers described herein, for example, those that enhance differentiation ability or those that are expressed over a long period of time. [Item 3-7-18D] The method according to any of the above items, wherein the marker, in the case of a culture medium, includes information relating to the identification of the component itself. [Item 3-7-19] A system for culturing cells, comprising: 1) a cell culture section for containing cells intended for culture; 2) a medium supply section for providing a culture medium or raw materials according to any of the above items; and 3) a cell culture condition adjustment section for adjusting culture conditions as necessary. [Item 3-7-20] A culture medium suitable for transporting cells containing the culture medium components according to any of the above items. [Item 3-7-21] A method for managing cell culture for cells suitable for culturing using the culture medium according to any of the above items, comprising: 1) providing information relating to the culture medium for cells provided by the user; 2) assigning identifiable labels to the cells and the culture medium; and 3) managing whether the cells and the culture medium are compatible by referring to the labels.

[0041] [Item 3-7-22] The use of (3) and (7) of Item 3-7-1 for culturing cells (preferably mesenchymal stem cells). [Item 3-7-23] The use of Item 3-7-22 for culturing cells (preferably mesenchymal stem cells), comprising the features of any one of Items 3-7-2 to 3-7-6C. [Item 3-7-24] A method for maintaining or improving the quality of cells (preferably mesenchymal stem cells) (preferably, the expression level of at least one selected from the group consisting of CD44, CD73, CD90 and CD105, and / or differentiation potential after long-term passage), comprising the step of contacting the cells in a culture medium comprising the components of Item 3-7-1. [Item 3-7-25] The method of Item 3-7-24, comprising the features of any one of Items 3-7-2 to 3-7-6C. [Item 3-7-26] Cells or cell populations described in any one of items 3-7-13 to 3-7-13AB, or differentiated cells or cell populations described in item 3-7-16, for use in treatment. [Item 3-7-27] Cells or cell populations described in any one of items 3-7-13 to 3-7-13AB, or differentiated cells or cell populations described in item 3-7-16, for use in regenerative medicine or in the treatment of cartilage diseases. [Item 3-7-28] Use of cells or cell populations described in any one of items 3-7-13 to 3-7-13AB, or differentiated cells or cell populations described in item 3-7-16, in the manufacture of drugs for regenerative medicine or in the treatment of cartilage diseases. [Item 3-7-29] A method for performing regenerative medicine or treating cartilage disease in a subject in need thereof, comprising the step of administering to the subject an effective amount of cells or cell populations described in any one of items 3-7-13 to 3-7-13AB, or differentiated cells or cell populations described in item 3-7-16.

[0042] Form 4-5 [Item 4-5-1] A medium containing (4) and (5). [Item 4-5-2] The medium according to any one of the above items, containing (4') and (5'). [Item 4-5-3] The medium according to any one of the above items, containing (4'') and (5''). [Item 4-5-4] The medium according to any one of the above items, containing (4A) and (5A). [Item 4-5-5] The medium according to any one of the above items, containing (4B) and (5B). [Item 4-5-5A] The medium according to any one of the above items, containing any one or more of (4), (4'), (4''), (4A) and (4B), and any one or more of (5), (5'), (5''), (5A) and (5B). [Item 4-5-6] The medium according to any one of the above items, which is for culturing various stem cells such as mesenchymal stem cells. [Item 4-5-6A] The medium according to any one of the above items, which is for culturing mesenchymal stem cells. [Item 4-5-6B] The medium according to any one of the above items, which is a serum-free medium. [Item 4-5-6C] The medium according to any one of the above items, which is a medium not containing raw materials derived from different species. [Item 4-5-7] A method for producing the cells, including the step of culturing the cells using the medium according to any one of the above items. [Item 4-5-8] The culturing step in the method according to any one of the above items includes culturing under conditions satisfying at least one selected from the group consisting of a specific cell number range, a specific container, and a specific temperature. [Item 4-5-8A] The range of the cell number is such that it is not seeded at a low density when it is MSC, preferably ２ ~20000 cells / cm ２ or so, more preferably 500 - 1000 / cm ２ ~10000 / cm ２ and, for example, 2000 cells / cm ２The method according to any one of the above items, although often seeded before or after. [Item 4-5-8B] The method according to any one of the above items, wherein the range of the cell number is a specific value, such as 1500 cells / well, at the time of screening, and / or is carried out under conditions such as 4500 cells / well, which are estimated to be confluent. [Item 4-5-8C] The method according to any one of the above items, wherein the specific container is a container of a suitable material (including with or without coating). [Item 4-5-8D] The method according to any one of the above items, wherein the specific temperature is a suitable temperature (such as 25-37°C or 30°C). [Item 4-5-9] A cell culture apparatus comprising a culture medium according to any one of the above items or means for providing the culture medium, a container capable of containing the culture medium, and means for providing conditions for culturing cells as necessary. [Item 4-5-10] A method for preserving cells, comprising the step of preserving cells using the culture medium according to any one of the above items. [Item 4-5-11] A composition for preserving cells, comprising the component described in any one of the above items. [Item 4-5-12] A cell-containing composition comprising the component described in any one of the above items and cells. [Item 4-5-13] Cells cultured using the component described in any one of the above items. [Item 4-5-13A] The cells described in any one of the above items, wherein the expression of at least one selected from the group consisting of CD44, CD73, CD90, and CD105 is not significantly reduced. [Item 4-5-13B] A cell population comprising the cells described in any of the above items, wherein the expression of at least one selected from the group consisting of CD44, CD73, CD90, and CD105 is not significantly reduced even after long-term passage. [Item 4-5-13C] The marker is at least one of CD44, CD73, CD90, and CD105, and at least one of these does not decrease. Typically, CD44 does not decrease, but the others often decrease, and it is a desirable and advantageous characteristic that CD73 and CD90, or CD44, CD90 and CD105, do not decrease, in any one of the above items, a cell or cell population.[Item 4-5-13D] Cells or cell populations according to any one of the above items, in which high expression of CD90 is maintained. [Item 4-5-13E] Cells or cell populations according to any one of the above items, in which the expression of at least one of the markers (preferably at least one of CD73, CD90, and CD105) is not significantly reduced when the number of passages is 5 or more, preferably 6 or more, and more preferably 7 or more. [Item 4-5-13F] Cells or cell populations according to any one of the above items, in which the expression of at least one of the markers (preferably at least one of CD73, CD90, and CD105) is not significantly reduced when the number of passages is 8 or more. [Item 4-5-13G] Cells or cell populations according to any one of the above items, in which differentiation ability is maintained when the number of passages is 5 or more, preferably 6 or more, and more preferably 7 or more. [Item 4-5-13H] Cells or cell populations according to any one of the above items, in which differentiation ability is maintained when the number of passages is 8 or more. [Item 4-5-13I] A cell or cell population according to any one of the above items, wherein the expression of CD90, CD44, and CD73 is maintained at 70% or more, usually 80% or more, preferably 90% or more, and more preferably 95% or more, respectively, before passage. [Item 4-5-13J] A cell or cell population according to any one of the above items, wherein the expression of CD105 is maintained at 30% or more, usually 40% or more, preferably 50% or more, and more preferably 70% or more, before passage. [Item 4-5-13AA] The cell according to item 4-5-13, wherein the cell properties are improved (for example, cartilage differentiation ability is enhanced). [Item 4-5-13AB] A cell population containing about 50% or more, about 60% or more, about 70% or more, about 80% or more, about 90% or more, or more of the cells according to any one of items 4-5-13 to 4-5-13AA. [Item 4-5-14] A pharmaceutical product comprising cells described in any one of the above items. [Item 4-5-15] A method for differentiating cells, comprising the step of culturing cells obtained using a culture medium described in any one of the above items under conditions that induce differentiation. [Item 4-5-15A] The method according to any one of the above items, wherein the culture comprises culturing the cells after proliferation using a different differentiation medium while confirming their differentiation ability.[Item 4-5-15B] The method according to any one of the above items, wherein the conditions under which the differentiation is induced also include differentiation conditions that are commonly used for MSCs. [Item 4-5-15C] The method according to any one of the above items, wherein the cells are less likely to become cartilage and / or have high cartilage differentiation potential. [Item 4-5-16] Differentiated cells obtained by differentiating stem cells by the method according to any one of the above items. [Item 4-5-17] A method for treating or preventing a subject, comprising the step of administering an effective amount of the cells or the pharmaceutical described in any one of the above items to a subject in need thereof. [Item 4-5-18] A method for quality control of stem cells, comprising determining whether they have a coefficient C correlated with the culture medium described in any one of the above items. [Item 4-5-18A] The method according to any one of the above items, wherein the coefficient C, in the case of cells, includes a coefficient relating to cell markers. [Item 4-5-18B] The method according to any one of the above items, wherein the cell marker includes common markers, such as cell markers associated with proliferation. [Item 4-5-18C] The method according to any of the above items, wherein the cell marker includes markers described herein, for example, those that enhance differentiation ability or those that are expressed over a long period of time. [Item 4-5-18D] The method according to any of the above items, wherein the marker, in the case of a culture medium, includes information relating to the identification of the component itself. [Item 4-5-19] A system for culturing cells, comprising: 1) a cell culture section for containing cells intended for culture; 2) a culture medium supply section for providing a culture medium or raw materials according to any of the above items; and 3) a cell culture condition adjustment section for adjusting culture conditions as necessary. [Item 4-5-20] A culture medium suitable for transporting cells containing culture medium components according to any of the above items. [Item 4-5-21] A method for managing cell culture for cells suitable for culturing using a culture medium according to any of the above items, comprising: 1) providing information relating to the culture medium for cells provided by the user; 2) assigning identifiable labels to the cells and the culture medium; and 3) managing whether the cells and the culture medium are compatible by referring to the labels.

[0043] [Item 4-5-22] The use of (4) and (5) of Item 4-5-1 for culturing cells (preferably mesenchymal stem cells). [Item 4-5-23] The use of Item 4-5-22 for culturing cells (preferably mesenchymal stem cells), comprising the features of any one of Items 4-5-2 to 4-5-6C. [Item 4-5-24] A method for maintaining or improving the quality of cells (preferably mesenchymal stem cells) (preferably, the expression level of at least one selected from the group consisting of CD44, CD73, CD90 and CD105, and / or differentiation potential after long-term passage), comprising the step of contacting the cells in a culture medium containing the components of Item 4-5-1. [Item 4-5-25] The method of Item 4-5-24, comprising the features of any one of Items 4-5-2 to 4-5-6C. [Item 4-5-26] Cells or cell populations described in any one of items 4-5-13 to 4-5-13AB, or differentiated cells or cell populations described in item 4-5-16, for use in treatment. [Item 4-5-27] Cells or cell populations described in any one of items 4-5-13 to 4-5-13AB, or differentiated cells or cell populations described in item 4-5-16, for use in regenerative medicine or in the treatment of cartilage diseases. [Item 4-5-28] Use of cells or cell populations described in any one of items 4-5-13 to 4-5-13AB, or differentiated cells or cell populations described in item 4-5-16, in the manufacture of drugs for regenerative medicine or in the treatment of cartilage diseases. [Item 4-5-29] A method for performing regenerative medicine or treating cartilage disease in a subject in need thereof, comprising the step of administering to the subject an effective amount of cells or cell populations described in any one of items 4-5-13 to 4-5-13AB, or differentiated cells or cell populations described in item 4-5-16.

[0044] Form 4-6 [Item 4-6-1] A culture medium comprising (4) and (6). [Item 4-6-2] A culture medium according to any of the above items, comprising (4') and (6'). [Item 4-6-3] A culture medium according to any one of the above items, comprising (4") and (6"). [Item 4-6-4] A culture medium according to any one of the above items, comprising (4A) and (6A). [Item 4-6-5] A culture medium according to any one of the above items, comprising (4B) and (6B). [Item 4-6-6A] A culture medium according to any one of the above items, comprising one or more of (4), (4'), (4"), (4A) and (4B), and one or more of (6), (6'), (6"), (6A) and (6B). [Item 4-6-6] A culture medium according to any one of the above items, for culturing various stem cells such as mesenchymal stem cells. [Item 4-6-6] A culture medium according to any one of the above items, for culturing various stem cells such as mesenchymal stem cells. [Item 4-6-6A] A culture medium according to any one of the above items, for culturing mesenchymal stem cells. [Item 4-6-6B] A serum-free culture medium according to any one of the above items. [Item 4-6-6C] A culture medium according to any one of the above items, which does not contain heterologous raw materials. [Item 4-6-7] A method for producing cells, comprising the step of culturing cells using the culture medium according to any one of the above items. [Item 4-6-8] The method according to any one of the above items, wherein the culturing step comprises culturing under conditions that satisfy at least one selected from the group consisting of a specific range of cell numbers, a specific container, and a specific temperature. [Item 4-6-8A] The range of cell numbers is such that MSCs are not seeded at low density, preferably 500 cells / cm². ２ ~20000 cells / cm ２ That's good enough, and more preferably, 500-1000 / cm ２ ~10,000 / cm ２ For example, 2000 cells / cm² ２The method according to any one of the above items, although often seeded before or after. [Item 4-6-8B] The method according to any one of the above items, wherein the range of the cell number is adopted at the time of screening, such as 1500 cells / well, and / or carried out under conditions such as 4500 cells / well which are estimated to become confluent. [Item 4-6-8C] The method according to any one of the above items, wherein the specific container is a container of a suitable material (including with or without coating). [Item 4-6-8D] The method according to any one of the above items, wherein the specific temperature is a suitable temperature (such as 25-37°C or 30°C). [Item 4-6-9] A cell culture apparatus comprising a culture medium according to any one of the above items or means for providing the culture medium, a container capable of containing the culture medium, and means for providing conditions for culturing cells as necessary. [Item 4-6-10] A method for preserving cells, comprising the step of preserving cells using the culture medium according to any one of the above items. [Item 4-6-11] A composition for preserving cells, comprising the component described in any one of the above items. [Item 4-6-12] A cell-containing composition comprising the component described in any one of the above items and cells. [Item 4-6-13] Cells cultured using the component described in any one of the above items. [Item 4-6-13A] The cells described in any one of the above items, wherein the expression of at least one selected from the group consisting of CD44, CD73, CD90, and CD105 is not significantly reduced. [Item 4-6-13B] A cell population comprising the cells described in any of the above items, wherein the expression of at least one selected from the group consisting of CD44, CD73, CD90, and CD105 is not significantly reduced even after long-term passage. [Item 4-6-13C] The marker is at least one of CD44, CD73, CD90, and CD105, and at least one of these does not decrease. Typically, CD44 does not decrease, but the others often decrease, and it is a desirable and advantageous characteristic that CD73 and CD90, or CD44, CD90 and CD105, do not decrease, in any one of the above items, a cell or cell population.[Item 4-6-13D] Cells or cell populations according to any one of the above items, in which high expression of CD90 is maintained. [Item 4-6-13E] Cells or cell populations according to any one of the above items, in which the expression of at least one of the markers (preferably at least one of CD73, CD90, and CD105) is not significantly reduced when the number of passages is 5 or more, preferably 6 or more, and more preferably 7 or more. [Item 4-6-13F] Cells or cell populations according to any one of the above items, in which the expression of at least one of the markers (preferably at least one of CD73, CD90, and CD105) is not significantly reduced when the number of passages is 8 or more. [Item 4-6-13G] Cells or cell populations according to any one of the above items, in which differentiation ability is maintained when the number of passages is 5 or more, preferably 6 or more, and more preferably 7 or more. [Item 4-6-13H] Cells or cell populations according to any one of the above items, in which differentiation ability is maintained when the number of passages is 8 or more. [Item 4-6-13I] A cell or cell population according to any one of the above items, wherein the expression of CD90, CD44, and CD73 is maintained at 70% or more, usually 80% or more, preferably 90% or more, and more preferably 95% or more, respectively, before passage. [Item 4-6-13J] A cell or cell population according to any one of the above items, wherein the expression of CD105 is maintained at 30% or more, usually 40% or more, preferably 50% or more, and more preferably 70% or more, before passage. [Item 4-6-13AA] The cell according to item 4-6-13, wherein the cell properties are improved (for example, cartilage differentiation ability is enhanced). [Item 4-6-13AB] A cell population containing about 50% or more, about 60% or more, about 70% or more, about 80% or more, about 90% or more, or more, of the cells according to any one of items 4-6-13 to 4-6-13AA. [Item 4-6-14] A pharmaceutical product comprising cells described in any one of the above items. [Item 4-6-15] A method for differentiating cells, comprising the step of culturing cells obtained using a culture medium described in any one of the above items under conditions that induce differentiation. [Item 4-6-15A] The method according to any one of the above items, wherein the culture comprises culturing the cells after proliferation using a different differentiation medium while confirming their differentiation ability.[Item 4-6-15B] The method according to any one of the above items, wherein the conditions under which the differentiation is induced also include differentiation conditions that are commonly used for MSCs. [Item 4-6-15C] The method according to any one of the above items, wherein the cells are less likely to become cartilage and / or have high cartilage differentiation potential. [Item 4-6-16] Differentiated cells obtained by differentiating stem cells by the method according to any one of the above items. [Item 4-6-17] A method for treating or preventing a subject, comprising the step of administering an effective amount of the cells or the pharmaceutical described in any one of the above items to a subject in need thereof. [Item 4-6-18] A method for quality control of stem cells, comprising determining whether they have a coefficient C correlated with the culture medium described in any one of the above items. [Item 4-6-18A] The method according to any one of the above items, wherein the coefficient C, in the case of cells, includes a coefficient relating to cell markers. [Item 4-6-18B] The method according to any one of the above items, wherein the cell marker includes common markers, such as cell markers associated with proliferation. [Item 4-6-18C] The method according to any of the above items, wherein the cell marker includes markers described herein, for example, those that enhance differentiation ability or those that are expressed over a long period of time. [Item 4-6-18D] The method according to any of the above items, wherein the marker, in the case of a culture medium, includes information relating to the identification of the component itself. [Item 4-6-19] A system for culturing cells, comprising: 1) a cell culture section for containing cells intended for culture; 2) a medium supply section for providing a culture medium or raw materials according to any of the above items; and 3) a cell culture condition adjustment section for adjusting culture conditions as necessary. [Item 4-6-20] A culture medium suitable for transporting cells containing the culture medium components described in any of the above items. [Item 4-6-21] A method for managing cell culture for cells suitable for culturing using the culture medium described in any of the above items, comprising: 1) providing information relating to the culture medium for cells provided by the user; 2) assigning identifiable labels to the cells and the culture medium; and 3) managing whether the cells and the culture medium are compatible by referring to the labels.

[0045] [Item 4-6-22] The use of (4) and (6) described in Item 4-6-1 for culturing cells (preferably mesenchymal stem cells). [Item 4-6-23] The use of Item 4-6-22 for culturing cells (preferably mesenchymal stem cells), comprising the features of any one of Items 4-6-2 to 4-6-6C. [Item 4-6-24] A method for maintaining or improving the quality of cells (preferably mesenchymal stem cells) (preferably, the expression level of at least one selected from the group consisting of CD44, CD73, CD90 and CD105, and / or differentiation potential after long-term passage), comprising the step of contacting the cells in a culture medium containing the components described in Item 4-6-1. [Item 4-6-25] The method according to Item 4-6-24, comprising the features of any one of Items 4-6-2 to 4-6-6C. [Item 4-6-26] Cells or cell populations described in any one of items 4-6-13 to 4-6-13AB, or differentiated cells or cell populations described in item 4-6-16, for use in treatment. [Item 4-6-27] Cells or cell populations described in any one of items 4-6-13 to 4-6-13AB, or differentiated cells or cell populations described in item 4-6-16, for use in regenerative medicine or in the treatment of cartilage diseases. [Item 4-6-28] Use of cells or cell populations described in any one of items 4-6-13 to 4-6-13AB, or differentiated cells or cell populations described in item 4-6-16, in the manufacture of drugs for regenerative medicine or in the treatment of cartilage diseases. [Item 4-6-29] A method for performing regenerative medicine or treating cartilage disease in a subject in need thereof, comprising the step of administering to the subject an effective amount of cells or cell populations described in any one of items 4-6-13 to 4-6-13AB, or differentiated cells or cell populations described in item 4-6-16.

[0046] Form 4-7 [Item 4-7-1] A medium containing (4) and (7). [Item 4-7-2] The medium according to any one of the above items, containing (4') and (7'). [Item 4-7-3] The medium according to any one of the above items, containing (4'') and (7''). [Item 4-7-4] The medium according to any one of the above items, containing (4A) and (7A). [Item 4-7-5] The medium according to any one of the above items, containing (4B) and (7B). [Item 4-7-5A] The medium according to any one of the above items, containing any one or more of (4), (4'), (4''), (4A) and (4B) and any one or more of (7), (7'), (7''), (7A) and (7B). [Item 4-7-6] The medium according to any one of the above items, which is for culturing various stem cells such as mesenchymal stem cells. [Item 4-7-6A] The medium according to any one of the above items, which is for culturing mesenchymal stem cells. [Item 4-7-6B] The medium according to any one of the above items, which is a serum-free medium. [Item 4-7-6C] The medium according to any one of the above items, which is a medium not containing raw materials derived from different species. [Item 4-7-7] A method for producing the cells, including the step of culturing the cells using the medium according to any one of the above items. [Item 4-7-8] The culturing step in the method according to any one of the above items includes culturing under conditions satisfying at least one selected from the group consisting of a specific cell number range, a specific container, and a specific temperature. [Item 4-7-8A] The range of the cell number is such that it is not seeded at a low density for MSC, preferably about ２ 500 cells / cm ２ to 20,000 cells / cm ２ is fine, more preferably 500 - 1000 / cm ２ to 10,000 / cm ２ is, for example, 2000 cells / cm ２[Item 4-7-8B] The method according to any one of the above items, wherein the seeding is often performed before and after the culture. [Item 4-7-8B] The method according to any one of the above items, wherein the range of the cell number is adopted at the time of screening, such as 1500 cells / well, and / or performed under conditions such as 4500 cells / well, which are estimated to become confluent. [Item 4-7-8C] The method according to any one of the above items, wherein the specific container is a container of appropriate material (including with or without coating). [Item 4-7-8D] The method according to any one of the above items, wherein the specific temperature is an appropriate temperature (such as 25-37°C or 30°C). [Item 4-7-9] A cell culture apparatus comprising a culture medium according to any one of the above items or means for providing the culture medium, a container capable of containing the culture medium, and means for providing conditions for culturing cells as necessary. [Item 4-7-10] A method for preserving cells, comprising the step of preserving cells using the culture medium according to any one of the above items. [Item 4-7-11] A composition for preserving cells, comprising the component described in any one of the above items. [Item 4-7-12] A cell-containing composition comprising the component described in any one of the above items and cells. [Item 4-7-13] Cells cultured using the component described in any one of the above items. [Item 4-7-13A] The cells described in any one of the above items, wherein the expression of at least one selected from the group consisting of CD44, CD73, CD90, and CD105 is not significantly reduced. [Item 4-7-13B] A cell population comprising the cells described in any of the above items, wherein the expression of at least one selected from the group consisting of CD44, CD73, CD90, and CD105 is not significantly reduced even after long-term passage. [Item 4-7-13C] The marker is at least one of CD44, CD73, CD90, and CD105, and at least one of these does not decrease. Typically, CD44 does not decrease, but the others often decrease, and it is a desirable and advantageous characteristic that CD73 and CD90, or CD44, CD90 and CD105, do not decrease, in any one of the above items, a cell or cell population.[Item 4-7-13D] Cells or cell populations according to any one of the above items, in which high expression of CD90 is maintained. [Item 4-7-13E] Cells or cell populations according to any one of the above items, in which the expression of at least one of the markers (preferably at least one of CD73, CD90, and CD105) is not significantly reduced when the number of passages is 5 or more, preferably 6 or more, and more preferably 7 or more. [Item 4-7-13F] Cells or cell populations according to any one of the above items, in which the expression of at least one of the markers (preferably at least one of CD73, CD90, and CD105) is not significantly reduced when the number of passages is 8 or more. [Item 4-7-13G] Cells or cell populations according to any one of the above items, in which differentiation ability is maintained when the number of passages is 5 or more, preferably 6 or more, and more preferably 7 or more. [Item 4-7-13H] Cells or cell populations according to any one of the above items, in which differentiation ability is maintained when the number of passages is 8 or more. [Item 4-7-13I] A cell or cell population according to any one of the above items, wherein the expression of CD90, CD44, and CD73 is maintained at 70% or more, usually 80% or more, preferably 90% or more, and more preferably 95% or more, respectively, before passage. [Item 4-7-13J] A cell or cell population according to any one of the above items, wherein the expression of CD105 is maintained at 30% or more, usually 40% or more, preferably 50% or more, and more preferably 70% or more, before passage. [Item 4-7-13AA] The cell according to item 4-7-13, wherein the cell properties are improved (for example, cartilage differentiation ability is enhanced). [Item 4-7-13AB] A cell population containing about 50% or more, about 60% or more, about 70% or more, about 80% or more, about 90% or more, or more of the cells according to any one of items 4-7-13 to 4-7-13AA. [Item 4-7-14] A pharmaceutical product comprising cells described in any one of the above items. [Item 4-7-15] A method for differentiating cells, comprising the step of culturing cells obtained using a culture medium described in any one of the above items under conditions that induce differentiation. [Item 4-7-15A] The method according to any one of the above items, wherein the culture comprises culturing the cells after proliferation using a different differentiation medium while confirming their differentiation ability.[Item 4-7-15B] The method according to any one of the above items, wherein the conditions under which the differentiation is induced also include differentiation conditions that are commonly used for MSCs. [Item 4-7-15C] The method according to any one of the above items, wherein the cells are less likely to become cartilage and / or have high cartilage differentiation potential. [Item 4-7-16] Differentiated cells obtained by differentiating stem cells by the method according to any one of the above items. [Item 4-7-17] A method for treating or preventing a subject, comprising the step of administering an effective amount of the cells or the pharmaceutical described in any one of the above items to a subject in need thereof. [Item 4-7-18] A method for quality control of stem cells, comprising determining whether they have a coefficient C correlated with the culture medium described in any one of the above items. [Item 4-7-18A] The method according to any one of the above items, wherein the coefficient C, in the case of cells, includes a coefficient relating to cell markers. [Item 4-7-18B] The method according to any one of the above items, wherein the cell marker includes common markers, such as cell markers related to proliferation. [Item 4-7-18C] The method according to any of the above items, wherein the cell marker includes markers described herein, for example, those that enhance differentiation ability or those that exhibit long-term expression. [Item 4-7-18D] The method according to any of the above items, wherein the marker, in the case of a culture medium, includes information relating to the identification of the component itself. [Item 4-7-19] A system for culturing cells, comprising: 1) a cell culture section for containing cells intended for culture; 2) a culture medium supply section for providing a culture medium or its raw materials as described in any of the above items; and 3) a cell culture condition adjustment section for adjusting culture conditions as necessary. [Item 4-7-20] A culture medium suitable for transporting cells containing culture medium components as described in any of the above items. [Item 4-7-21] A method for managing cell culture for cells suitable for culturing using a culture medium as described in any of the above items, comprising: 1) providing information relating to the culture medium for cells provided by the user; 2) assigning identifiable labels to the cells and the culture medium; and 3) managing whether the cells and the culture medium are compatible by referring to the labels.

[0047] [Item 4-7-22] The use of (4) and (7) of Item 4-7-1 for culturing cells (preferably mesenchymal stem cells). [Item 4-7-23] The use of Item 4-7-22 for culturing cells (preferably mesenchymal stem cells), comprising the features of any one of Items 4-7-2 to 4-7-6C. [Item 4-7-24] A method for maintaining or improving the quality of cells (preferably mesenchymal stem cells) (preferably, the expression level of at least one selected from the group consisting of CD44, CD73, CD90 and CD105, and / or differentiation potential after long-term passage), the method comprising the step of contacting the cells in a culture medium comprising the components of Item 4-7-1. [Item 4-7-25] The method of Item 4-7-24, comprising the features of any one of Items 4-7-2 to 4-7-6C. [Item 4-7-26] Cells or cell populations described in any one of items 4-7-13 to 4-7-13AB, or differentiated cells or cell populations described in item 4-7-16, for use in treatment. [Item 4-7-27] Cells or cell populations described in any one of items 4-7-13 to 4-7-13AB, or differentiated cells or cell populations described in item 4-7-16, for use in regenerative medicine or in the treatment of cartilage diseases. [Item 4-7-28] Use of cells or cell populations described in any one of items 4-7-13 to 4-7-13AB, or differentiated cells or cell populations described in item 4-7-16, in the manufacture of drugs for regenerative medicine or in the treatment of cartilage diseases. [Item 4-7-29] A method for performing regenerative medicine or treating cartilage disease in a subject in need thereof, comprising the step of administering to the subject an effective amount of cells or cell populations described in any one of items 4-7-13 to 4-7-13AB, or differentiated cells or cell populations described in item 4-7-16.

[0048] Form 5-6 [Item 5-6-1] A culture medium comprising (5) and (6). [Item 5-6-2] A culture medium according to any of the above items, comprising (5') and (6'). [Item 5-6-3] A culture medium according to any one of the above items, comprising (5") and (6"). [Item 5-6-4] A culture medium according to any one of the above items, comprising (5A) and (6A). [Item 5-6-5] A culture medium according to any one of the above items, comprising (5B) and (6B). [Item 5-6-6A] A culture medium according to any one of the above items, comprising one or more of (5), (5'), (5"), (5A) and (5B), and one or more of (6), (6'), (6"), (6A) and (6B). [Item 5-6-6] A culture medium according to any one of the above items, for culturing various stem cells such as mesenchymal stem cells. [Item 5-6-6A] A culture medium according to any one of the above items, for culturing mesenchymal stem cells. [Item 5-6-6B] A serum-free culture medium according to any one of the above items. [Item 5-6-6C] A culture medium according to any one of the above items, which does not contain heterologous raw materials. [Item 5-6-7] A method for producing cells, comprising the step of culturing cells using the culture medium according to any one of the above items. [Item 5-6-8] The method according to any one of the above items, wherein the culturing step comprises culturing under conditions that satisfy at least one selected from the group consisting of a specific range of cell numbers, a specific container, and a specific temperature. [Item 5-6-8A] The range of cell numbers is such that MSCs are not seeded at low densities, preferably 500 cells / cm². ２ ~20000 cells / cm ２ That's good enough, and more preferably, 500-1000 / cm ２ ~10,000 / cm ２ For example, 2000 cells / cm² ２The method according to any one of the above items, although often seeded before or after. [Item 5-6-8B] The method according to any one of the above items, wherein the range of the cell number is a specific value, such as 1500 cells / well, at the time of screening, and / or is carried out under conditions such as 4500 cells / well, which are estimated to become confluent. [Item 5-6-8C] The method according to any one of the above items, wherein the specific container is a container of a suitable material (including with or without coating). [Item 5-6-8D] The method according to any one of the above items, wherein the specific temperature is a suitable temperature (such as 25-37°C or 30°C). [Item 5-6-9] A cell culture apparatus comprising a culture medium according to any one of the above items or means for providing the culture medium, a container capable of containing the culture medium, and means for providing conditions for culturing cells as necessary. [Item 5-6-10] A method for preserving cells, comprising the step of preserving cells using the culture medium according to any one of the above items. [Item 5-6-11] A composition for preserving cells, comprising the component described in any one of the above items. [Item 5-6-12] A cell-containing composition comprising the component described in any one of the above items and cells. [Item 5-6-13] Cells cultured using the component described in any one of the above items. [Item 5-6-13A] The cells described in any one of the above items, wherein the expression of at least one selected from the group consisting of CD44, CD73, CD90, and CD105 is not significantly reduced. [Item 5-6-13B] A cell population comprising the cells described in any of the above items, wherein the expression of at least one selected from the group consisting of CD44, CD73, CD90, and CD105 is not significantly reduced even after long-term passage. [Item 5-6-13C] The marker is at least one of CD44, CD73, CD90, and CD105, and at least one of these does not decrease. Typically, CD44 does not decrease, but the others often decrease, and it is a desirable and advantageous characteristic that CD73 and CD90, or CD44, CD90 and CD105, do not decrease, in any one of the above items, a cell or cell population.[Item 5-6-13D] Cells or cell populations according to any one of the above items, in which high expression of CD90 is maintained. [Item 5-6-13E] Cells or cell populations according to any one of the above items, in which the expression of at least one of the markers (preferably at least one of CD73, CD90, and CD105) is not significantly reduced when the number of passages is 5 or more, preferably 6 or more, and more preferably 7 or more. [Item 5-6-13F] Cells or cell populations according to any one of the above items, in which the expression of at least one of the markers (preferably at least one of CD73, CD90, and CD105) is not significantly reduced when the number of passages is 8 or more. [Item 5-6-13G] Cells or cell populations according to any one of the above items, in which differentiation ability is maintained when the number of passages is 5 or more, preferably 6 or more, and more preferably 7 or more. [Item 5-6-13H] Cells or cell populations according to any one of the above items, in which differentiation ability is maintained when the number of passages is 8 or more. [Item 5-6-13I] A cell or cell population according to any one of the above items, wherein the expression of CD90, CD44, and CD73 is maintained at 70% or more, usually 80% or more, preferably 90% or more, and more preferably 95% or more, respectively, before passage. [Item 5-6-13J] A cell or cell population according to any one of the above items, wherein the expression of CD105 is maintained at 30% or more, usually 40% or more, preferably 50% or more, and more preferably 70% or more, before passage. [Item 5-6-13AA] The cell according to item 5-6-13, wherein the cell properties are improved (for example, cartilage differentiation ability is enhanced). [Item 5-6-13AB] A cell population containing about 50% or more, about 60% or more, about 70% or more, about 80% or more, about 90% or more, or more of the cells according to any one of items 5-6-13 to 5-6-13AA. [Item 5-6-14] A pharmaceutical product comprising cells described in any one of the above items. [Item 5-6-15] A method for differentiating cells, comprising the step of culturing cells obtained using a culture medium described in any one of the above items under conditions that induce differentiation. [Item 5-6-15A] The method according to any one of the above items, wherein the culture comprises culturing the cells after proliferation using a different differentiation medium while confirming their differentiation ability.[Item 5-6-15B] The method according to any one of the above items, wherein the conditions under which the differentiation is induced also include differentiation conditions that are commonly used for MSCs. [Item 5-6-15C] The method according to any one of the above items, wherein the cells are less likely to become cartilage and / or have high cartilage differentiation potential. [Item 5-6-16] Differentiated cells obtained by differentiating stem cells by the method according to any one of the above items. [Item 5-6-17] A method for treating or preventing a subject, comprising the step of administering an effective amount of the cells or the pharmaceutical described in any one of the above items to a subject in need thereof. [Item 5-6-18] A method for quality control of stem cells, comprising determining whether they have a coefficient C correlated with the culture medium described in any one of the above items. [Item 5-6-18A] The method according to any one of the above items, wherein the coefficient C, in the case of cells, includes a coefficient relating to cell markers. [Item 5-6-18B] The method according to any one of the above items, wherein the cell marker includes common markers, such as cell markers associated with proliferation. [Item 5-6-18C] The method according to any of the above items, wherein the cell marker includes markers described herein, such as those that enhance differentiation ability or those that exhibit long-term expression. [Item 5-6-18D] The method according to any of the above items, wherein the marker, in the case of a culture medium, includes information relating to the identification of the component itself. [Item 5-6-19] A system for culturing cells, comprising: 1) a cell culture section for containing cells intended for culture; 2) a culture medium supply section for providing a culture medium or its raw materials as described in any of the above items; and 3) a cell culture condition adjustment section for adjusting culture conditions as necessary. [Item 5-6-20] A culture medium suitable for transporting cells containing culture medium components as described in any of the above items. [Item 5-6-21] A method for managing cell culture for cells suitable for culturing using a culture medium as described in any of the above items, comprising: 1) providing information relating to the culture medium for cells provided by the user; 2) assigning identifiable labels to the cells and the culture medium; and 3) managing whether the cells and the culture medium are compatible by referring to the labels.

[0049] [Item 5-6-22] The use of (5) and (6) described in Item 5-6-1 for culturing cells (preferably mesenchymal stem cells). [Item 5-6-23] The use of Item 5-6-22 for culturing cells (preferably mesenchymal stem cells), comprising the features of any one of Items 5-6-2 to 5-6-6C. [Item 5-6-24] A method for maintaining or improving the quality of cells (preferably mesenchymal stem cells) (preferably, the expression level of at least one selected from the group consisting of CD44, CD73, CD90 and CD105, and / or differentiation potential after long-term passage), the method comprising the step of contacting the cells in a culture medium containing the components described in Item 5-6-1. [Item 5-6-25] The method of Item 5-6-24, comprising the features of any one of Items 5-6-2 to 5-6-6C. [Item 5-6-26] Cells or cell populations described in any one of items 5-6-13 to 5-6-13AB, or differentiated cells or cell populations described in item 5-6-16, for use in treatment. [Item 5-6-27] Cells or cell populations described in any one of items 5-6-13 to 5-6-13AB, or differentiated cells or cell populations described in item 5-6-16, for use in regenerative medicine or in the treatment of cartilage diseases. [Item 5-6-28] Use of cells or cell populations described in any one of items 5-6-13 to 5-6-13AB, or differentiated cells or cell populations described in item 5-6-16, in the manufacture of drugs for regenerative medicine or in the treatment of cartilage diseases. [Item 5-6-29] A method for performing regenerative medicine or treating cartilage disease in a subject in need thereof, comprising the step of administering to the subject an effective amount of cells or cell populations described in any one of items 5-6-13 to 5-6-13AB, or differentiated cells or cell populations described in item 5-6-16.

[0050] Form 5-7 [Item 5-7-1] A culture medium comprising (5) and (7). [Item 5-7-2] A culture medium according to any of the above items, comprising (5') and (7'). [Item 5-7-3] A culture medium according to any one of the above items, comprising (5") and (7"). [Item 5-7-4] A culture medium according to any one of the above items, comprising (5A) and (7A). [Item 5-7-5] A culture medium according to any one of the above items, comprising (5B) and (7B). [Item 5-7-5A] A culture medium according to any one of the above items, comprising one or more of (5), (5'), (5"), (5A) and (5B), and one or more of (7), (7'), (7"), (7A) and (7B). [Item 5-7-6] A culture medium according to any one of the above items, for culturing various stem cells such as mesenchymal stem cells. [Item 5-7-6A] A culture medium according to any one of the above items, for culturing mesenchymal stem cells. [Item 5-7-6B] A serum-free culture medium according to any one of the above items. [Item 5-7-6C] A culture medium according to any one of the above items, which does not contain heterologous raw materials. [Item 5-7-7] A method for producing cells, comprising the step of culturing cells using the culture medium according to any one of the above items. [Item 5-7-8] The method according to any one of the above items, wherein the culturing step comprises culturing under conditions that satisfy at least one selected from the group consisting of a specific range of cell numbers, a specific container, and a specific temperature. [Item 5-7-8A] The range of cell numbers is such that MSCs are not seeded at low densities, preferably 500 cells / cm². ２ ~20000 cells / cm ２ That's good enough, and more preferably, 500-1000 / cm ２ ~10,000 / cm ２ For example, 2000 cells / cm² ２The method according to any one of the above items, although often seeded before or after. [Item 5-7-8B] The method according to any one of the above items, wherein the range of the cell number is adopted at the time of screening, such as 1500 cells / well, and / or carried out under conditions such as 4500 cells / well, which are estimated to become confluent. [Item 5-7-8C] The method according to any one of the above items, wherein the specific container is a container of a suitable material (including with or without coating). [Item 5-7-8D] The method according to any one of the above items, wherein the specific temperature is a suitable temperature (such as 25-37°C or 30°C). [Item 5-7-9] A cell culture apparatus comprising a culture medium according to any one of the above items or means for providing the culture medium, a container capable of containing the culture medium, and means for providing conditions for culturing cells as necessary. [Item 5-7-10] A method for preserving cells, comprising the step of preserving cells using the culture medium according to any one of the above items. [Item 5-7-11] A composition for preserving cells, comprising the component described in any one of the above items. [Item 5-7-12] A cell-containing composition comprising the component described in any one of the above items and cells. [Item 5-7-13] Cells cultured using the component described in any one of the above items. [Item 5-7-13A] The cells described in any one of the above items, wherein the expression of at least one selected from the group consisting of CD44, CD73, CD90, and CD105 is not significantly reduced. [Item 5-7-13B] A cell population comprising the cells described in any of the above items, wherein the expression of at least one selected from the group consisting of CD44, CD73, CD90, and CD105 is not significantly reduced even after long-term passage. [Item 5-7-13C] The marker is at least one of CD44, CD73, CD90, and CD105, and at least one of these does not decrease. Typically, CD44 does not decrease, but the others often decrease, and it is a desirable and advantageous characteristic that CD73 and CD90, or CD44, CD90 and CD105, do not decrease, in any one of the above items, a cell or cell population.[Item 5-7-13D] Cells or cell populations according to any one of the above items, in which high expression of CD90 is maintained. [Item 5-7-13E] Cells or cell populations according to any one of the above items, in which the expression of at least one of the markers (preferably at least one of CD73, CD90, and CD105) is not significantly reduced when the number of passages is 5 or more, preferably 6 or more, and more preferably 7 or more. [Item 5-7-13F] Cells or cell populations according to any one of the above items, in which the expression of at least one of the markers (preferably at least one of CD73, CD90, and CD105) is not significantly reduced when the number of passages is 8 or more. [Item 5-7-13G] Cells or cell populations according to any one of the above items, in which differentiation ability is maintained when the number of passages is 5 or more, preferably 6 or more, and more preferably 7 or more. [Item 5-7-13H] Cells or cell populations according to any one of the above items, in which differentiation ability is maintained when the number of passages is 8 or more. [Item 5-7-13I] A cell or cell population according to any one of the above items, wherein the expression of CD90, CD44, and CD73 is maintained at 70% or more, usually 80% or more, preferably 90% or more, and more preferably 95% or more, respectively, before passage. [Item 5-7-13J] A cell or cell population according to any one of the above items, wherein the expression of CD105 is maintained at 30% or more, usually 40% or more, preferably 50% or more, and more preferably 70% or more, before passage. [Item 5-7-13AA] The cell according to item 5-7-13, wherein the properties of the cell are improved (for example, the ability to differentiate into cartilage is enhanced). [Item 5-7-13AB] A cell population containing about 50% or more, about 60% or more, about 70% or more, about 80% or more, about 90% or more, or more, of the cells according to any one of items 5-7-13 to 5-7-13AA. [Item 5-7-14] A pharmaceutical product comprising cells described in any one of the above items. [Item 5-7-15] A method for differentiating cells, comprising the step of culturing cells obtained using a culture medium described in any one of the above items under conditions that induce differentiation. [Item 5-7-15A] The method according to any one of the above items, wherein the culture comprises culturing the cells after proliferation using a different differentiation medium while confirming their differentiation ability.[Item 5-7-15B] The method according to any one of the above items, wherein the conditions under which the differentiation is induced also include differentiation conditions that are commonly used for MSCs. [Item 5-7-15C] The method according to any one of the above items, wherein the cells are less likely to become cartilage and / or have high cartilage differentiation potential. [Item 5-7-16] Differentiated cells obtained by differentiating stem cells by the method according to any one of the above items. [Item 5-7-17] A method for treating or preventing a subject, comprising the step of administering an effective amount of the cells or the pharmaceutical described in any one of the above items to a subject in need thereof. [Item 5-7-18] A method for quality control of stem cells, comprising determining whether they have a coefficient C correlated with the culture medium described in any one of the above items. [Item 5-7-18A] The method according to any one of the above items, wherein the coefficient C, in the case of cells, includes a coefficient relating to cell markers. [Item 5-7-18B] The method according to any one of the above items, wherein the cell marker includes common markers, such as cell markers related to proliferation. [Item 5-7-18C] The method according to any of the above items, wherein the cell marker includes markers described herein, for example, those that enhance differentiation ability or those that are expressed over a long period of time. [Item 5-7-18D] The method according to any of the above items, wherein the marker, in the case of a culture medium, includes information relating to the identification of the component itself. [Item 5-7-19] A system for culturing cells, comprising: 1) a cell culture section for containing cells intended for culture; 2) a medium supply section for providing a culture medium or raw materials according to any one of the above items; and 3) a cell culture condition adjustment section for adjusting culture conditions as necessary. [Item 5-7-20] A culture medium suitable for transporting cells containing the culture medium components according to any one of the above items. [Item 5-7-21] A method for managing cell culture for cells suitable for culturing using the culture medium according to any one of the above items, comprising: 1) providing information relating to the culture medium for cells provided by the user; 2) assigning identifiable labels to the cells and the culture medium; and 3) managing whether the cells and the culture medium are compatible by referring to the labels.

[0051] [Item 5-7-22] The use of (5) and (7) described in Item 5-7-1 for culturing cells (preferably mesenchymal stem cells). [Item 5-7-23] The use of Item 5-7-22 for culturing cells (preferably mesenchymal stem cells), comprising the features of any one of Items 5-7-2 to 5-7-6C. [Item 5-7-24] A method for maintaining or improving the quality of cells (preferably mesenchymal stem cells) (preferably, the expression level of at least one selected from the group consisting of CD44, CD73, CD90 and CD105, and / or differentiation potential after long-term passage), comprising the step of contacting the cells in a culture medium containing the components described in Item 5-7-1. [Item 5-7-25] The method according to Item 5-7-24, comprising the features of any one of Items 5-7-2 to 5-7-6C. [Item 5-7-26] Cells or cell populations described in any one of items 5-7-13 to 5-7-13AB, or differentiated cells or cell populations described in item 5-7-16, for use in treatment. [Item 5-7-27] Cells or cell populations described in any one of items 5-7-13 to 5-7-13AB, or differentiated cells or cell populations described in item 5-7-16, for use in regenerative medicine or in the treatment of cartilage diseases. [Item 5-7-28] Use of cells or cell populations described in any one of items 5-7-13 to 5-7-13AB, or differentiated cells or cell populations described in item 5-7-16, in the manufacture of drugs for regenerative medicine or in the treatment of cartilage diseases. [Item 5-7-29] A method for performing regenerative medicine or treating cartilage disease in a subject in need thereof, comprising the step of administering to the subject an effective amount of cells or cell populations described in any one of items 5-7-13 to 5-7-13AB, or differentiated cells or cell populations described in item 5-7-16.

[0052] Form 6-7 [Item 6-7-1] A culture medium comprising (6) and (7). [Item 6-7-2] A culture medium according to any of the above items, comprising (6') and (7'). [Item 6-7-3] A culture medium according to any one of the above items, comprising (6") and (7"). [Item 6-7-4] A culture medium according to any one of the above items, comprising (6A) and (7A). [Item 6-7-5] A culture medium according to any one of the above items, comprising (6B) and (7B). [Item 6-7-5A] A culture medium according to any one of the above items, comprising one or more of (6), (6'), (6"), (6A) and (6B), and one or more of (7), (7'), (7"), (7A) and (7B). [Item 6-7-6] A culture medium according to any one of the above items, for culturing various stem cells such as mesenchymal stem cells. [Item 6-7-6A] A culture medium according to any one of the above items, for culturing mesenchymal stem cells. [Item 6-7-6B] A serum-free culture medium according to any one of the above items. [Item 6-7-6C] A culture medium according to any one of the above items, which does not contain heterologous raw materials. [Item 6-7-7] A method for producing cells, comprising the step of culturing cells using the culture medium according to any one of the above items. [Item 6-7-8] The method according to any one of the above items, wherein the culturing step comprises culturing under conditions that satisfy at least one selected from the group consisting of a specific range of cell numbers, a specific container, and a specific temperature. [Item 6-7-8A] The range of cell numbers is such that MSCs are not seeded at low densities, preferably 500 cells / cm². ２ ~20000 cells / cm ２ That's good enough, and more preferably, 500-1000 / cm ２ ~10,000 / cm ２ For example, 2000 cells / cm² ２The method according to any one of the above items, although often seeded before or after. [Item 6-7-8B] The method according to any one of the above items, wherein the range of the cell number is a specific value, such as 1500 cells / well, at the time of screening, and / or is carried out under conditions such as 4500 cells / well, which are estimated to be confluent. [Item 6-7-8C] The method according to any one of the above items, wherein the specific container is a container of a suitable material (including with or without coating). [Item 6-7-8D] The method according to any one of the above items, wherein the specific temperature is a suitable temperature (such as 25-37°C or 30°C). [Item 6-7-9] A cell culture apparatus comprising a culture medium according to any one of the above items or means for providing the culture medium, a container capable of containing the culture medium, and means for providing conditions for culturing cells as necessary. [Item 6-7-10] A method for preserving cells, comprising the step of preserving cells using the culture medium according to any one of the above items. [Item 6-7-11] A composition for preserving cells, comprising the component described in any one of the above items. [Item 6-7-12] A cell-containing composition comprising the component described in any one of the above items and cells. [Item 6-7-13] Cells cultured using the component described in any one of the above items. [Item 6-7-13A] The cells described in any one of the above items, wherein the expression of at least one selected from the group consisting of CD44, CD73, CD90, and CD105 is not significantly reduced. [Item 6-7-13B] A cell population comprising the cells described in any of the above items, wherein the expression of at least one selected from the group consisting of CD44, CD73, CD90, and CD105 is not significantly reduced even after long-term passage. [Item 6-7-13C] The marker is at least one of CD44, CD73, CD90, and CD105, and at least one of these does not decrease. Typically, CD44 does not decrease, but the others often decrease, and it is a desirable and advantageous characteristic that CD73 and CD90, or CD44, CD90 and CD105, do not decrease, in any one of the above items, a cell or cell population.[Item 6-7-13D] Cells or cell populations according to any one of the above items, in which high expression of CD90 is maintained. [Item 6-7-13E] Cells or cell populations according to any one of the above items, in which the expression of at least one of the markers (preferably at least one of CD73, CD90, and CD105) is not significantly reduced when the number of passages is 5 or more, preferably 6 or more, and more preferably 7 or more. [Item 6-7-13F] Cells or cell populations according to any one of the above items, in which the expression of at least one of the markers (preferably at least one of CD73, CD90, and CD105) is not significantly reduced when the number of passages is 8 or more. [Item 6-7-13G] Cells or cell populations according to any one of the above items, in which differentiation ability is maintained when the number of passages is 5 or more, preferably 6 or more, and more preferably 7 or more. [Item 6-7-13H] Cells or cell populations according to any one of the above items, in which differentiation ability is maintained when the number of passages is 8 or more. [Item 6-7-13I] A cell or cell population according to any one of the above items, wherein the expression of CD90, CD44, and CD73 is maintained at 70% or more, usually 80% or more, preferably 90% or more, and more preferably 95% or more, respectively, before passage. [Item 6-7-13J] A cell or cell population according to any one of the above items, wherein the expression of CD105 is maintained at 30% or more, usually 40% or more, preferably 50% or more, and more preferably 70% or more, before passage. [Item 6-7-13AA] The cell according to item 6-7-13, wherein the cell properties are improved (for example, cartilage differentiation ability is enhanced). [Item 6-7-13AB] A cell population containing about 50% or more, about 60% or more, about 70% or more, about 80% or more, about 90% or more, or more of the cells according to any one of items 6-7-13 to 6-7-13AA. [Item 6-7-14] A pharmaceutical product comprising cells described in any one of the above items. [Item 6-7-15] A method for differentiating cells, comprising the step of culturing cells obtained using a culture medium described in any one of the above items under conditions that induce differentiation. [Item 6-7-15A] The method according to any one of the above items, wherein the culture comprises culturing the cells after proliferation using a different differentiation medium while confirming their differentiation ability.[Item 6-7-15B] The method according to any one of the above items, wherein the conditions under which the differentiation is induced also include differentiation conditions that are commonly used for MSCs. [Item 6-7-15C] The method according to any one of the above items, wherein the cells are less likely to become cartilage and / or have high cartilage differentiation potential. [Item 6-7-16] Differentiated cells obtained by differentiating stem cells by the method according to any one of the above items. [Item 6-7-17] A method for treating or preventing a subject, comprising the step of administering an effective amount of the cells or the pharmaceutical described in any one of the above items to a subject in need thereof. [Item 6-7-18] A method for quality control of stem cells, comprising determining whether they have a coefficient C correlated with the culture medium described in any one of the above items. [Item 6-7-18A] The method according to any one of the above items, wherein the coefficient C, in the case of cells, includes a coefficient relating to cell markers. [Item 6-7-18B] The method according to any one of the above items, wherein the cell marker includes common markers, such as cell markers associated with proliferation. [Item 6-7-18C] The method according to any of the above items, wherein the cell marker includes markers described herein, for example, those that enhance differentiation ability or those that are expressed over a long period of time. [Item 6-7-18D] The method according to any of the above items, wherein the marker, in the case of a culture medium, includes information relating to the identification of the component itself. [Item 6-7-19] A system for culturing cells, comprising: 1) a cell culture section for containing cells intended for culture; 2) a medium supply section for providing a culture medium or raw materials according to any of the above items; and 3) a cell culture condition adjustment section for adjusting culture conditions as necessary. [Item 6-7-20] A culture medium suitable for transporting cells containing the culture medium components according to any of the above items. [Item 6-7-21] A method for managing cell culture for cells suitable for culturing using a culture medium according to any of the above items, comprising: 1) providing information relating to the culture medium for cells provided by the user; 2) assigning identifiable labels to the cells and the culture medium; and 3) managing whether the cells and the culture medium are compatible by referring to the labels.

[0053] [Item 6-7-22] The use of (6) and (7) described in Item 6-7-1 for culturing cells (preferably mesenchymal stem cells). [Item 6-7-23] The use of Item 6-7-22 for culturing cells (preferably mesenchymal stem cells), comprising the features of any one of Items 6-7-2 to 6-7-6C. [Item 6-7-24] A method for maintaining or improving the quality of cells (preferably mesenchymal stem cells) (preferably, the expression level of at least one selected from the group consisting of CD44, CD73, CD90 and CD105, and / or differentiation potential after long-term passage), comprising the step of contacting the cells in a culture medium comprising the components described in Item 6-7-1. [Item 6-7-25] The method according to Item 6-7-24, comprising the features of any one of Items 6-7-2 to 6-7-6C. [Item 6-7-26] Cells or cell populations described in any one of items 6-7-13 to 6-7-13AB, or differentiated cells or cell populations described in item 6-7-16, for use in treatment. [Item 6-7-27] Cells or cell populations described in any one of items 6-7-13 to 6-7-13AB, or differentiated cells or cell populations described in item 6-7-16, for use in regenerative medicine or in the treatment of cartilage diseases. [Item 6-7-28] Use of cells or cell populations described in any one of items 6-7-13 to 6-7-13AB, or differentiated cells or cell populations described in item 6-7-16, in the manufacture of drugs for regenerative medicine or in the treatment of cartilage diseases. [Item 6-7-29] A method for performing regenerative medicine or treating cartilage disease in a subject in need thereof, comprising the step of administering to the subject an effective amount of cells or cell populations described in any one of items 6-7-13 to 6-7-13AB, or differentiated cells or cell populations described in item 6-7-16.

[0054] Examples including Forms 1-2-3 (1), (2), and (3): [Item 1-2-3-1] A culture medium including (1), (2), and (3). [Item 1-2-3-2] A culture medium according to any of the above items, including (1'), (2'), and (3'). [Item 1-2-3-3] A culture medium according to any one of the above items, including (1"), (2"), and (3"). [Item 1-2-3-4] A culture medium according to any one of the above items, including (1A), (2A), and (3A). [Item 1-2-3-5] A culture medium according to any one of the above items, including (1B), (2B), and (3B). [Item 1-2-3-5A] A culture medium according to any one of the above items, including one or more of (1), (1'), (1"), (1A), and (1B), one or more of (2), (2'), (2"), (2A), and (2B), and one or more of (3), (3'), (3"), (3A), and (3B). [Item 1-2-3-6] A culture medium according to any one of the above items, for culturing various stem cells such as mesenchymal stem cells. [Item 1-2-3-6A] A culture medium according to any one of the above items, for culturing mesenchymal stem cells. [Item 1-2-3-6B] A serum-free culture medium according to any one of the above items. [Item 1-2-3-6C] A culture medium according to any one of the above items, which does not contain heterologous raw materials. [Item 1-2-3-7] A method for producing cells, comprising the step of culturing cells using the culture medium according to any one of the above items. [Item 1-2-3-8] The method according to any one of the above items, wherein the culturing step comprises culturing under conditions that satisfy at least one selected from the group consisting of a specific range of cell numbers, a specific container, and a specific temperature. [Item 1-2-3-8A] The range of cell numbers is such that MSCs are not seeded at low densities, and is preferably 500 cells / cm². ２ ~20000 cells / cm ２ That's good enough, and more preferably, 500-1000 / cm ２ ~10,000 / cm ２ For example, 2000 cells / cm² ２The method according to any one of the above items, although often seeded before or after. [Item 1-2-3-8B] The method according to any one of the above items, wherein the range of the cell number is adopted at the time of screening, such as 1500 cells / well, and / or carried out under conditions such as 4500 cells / well which are estimated to become confluent. [Item 1-2-3-8C] The method according to any one of the above items, wherein the specific container is a container of a suitable material (including with or without coating). [Item 1-2-3-8D] The method according to any one of the above items, wherein the specific temperature is a suitable temperature (such as 25-37°C or 30°C). [Item 1-2-3-9] A cell culture apparatus comprising a culture medium according to any one of the above items or means for providing the culture medium, a container capable of containing the culture medium, and means for providing conditions for culturing cells as necessary. [Item 1-2-3-10] A method for preserving cells, comprising the step of preserving cells using the culture medium according to any one of the above items. [Item 1-2-3-11] A composition for preserving cells, comprising the component described in any one of the above items. [Item 1-2-3-12] A cell-containing composition comprising the component described in any one of the above items and cells. [Item 1-2-3-13] Cells cultured using the component described in any one of the above items. [Item 1-2-3-13A] The cells described in any one of the above items, wherein the expression of at least one selected from the group consisting of CD44, CD73, CD90, and CD105 is not significantly reduced. [Item 1-2-3-13B] A cell population comprising the cells described in any of the above items, wherein the expression of at least one selected from the group consisting of CD44, CD73, CD90, and CD105 is not significantly reduced even after long-term passage. [Item 1-2-3-13C] The marker is at least one of CD44, CD73, CD90, and CD105, and at least one of these does not decrease. Typically, CD44 does not decrease, but the others often decrease, and it is a desirable and advantageous characteristic that CD73 and CD90, or CD44, CD90 and CD105, do not decrease, in any one of the above items, a cell or cell population.[Item 1-2-3-13D] Cells or cell populations according to any one of the above items, in which high expression of CD90 is maintained. [Item 1-2-3-13E] Cells or cell populations according to any one of the above items, in which the expression of at least one of the markers (preferably at least one of CD73, CD90, and CD105) is not significantly reduced when the number of passages is 5 or more, preferably 6 or more, and more preferably 7 or more. [Item 1-2-3-13F] Cells or cell populations according to any one of the above items, in which the expression of at least one of the markers (preferably at least one of CD73, CD90, and CD105) is not significantly reduced when the number of passages is 8 or more. [Item 1-2-3-13G] Cells or cell populations according to any one of the above items, in which differentiation ability is maintained when the number of passages is 5 or more, preferably 6 or more, and more preferably 7 or more. [Item 1-2-3-13H] Cells or cell populations according to any one of the above items, in which differentiation ability is maintained when the number of passages is 8 or more. [Item 1-2-3-13I] A cell or cell population according to any one of the above items, wherein the expression of CD90, CD44, and CD73 is maintained at 70% or more, usually 80% or more, preferably 90% or more, and more preferably 95% or more, respectively, before passage. [Item 1-2-3-13J] A cell or cell population according to any one of the above items, wherein the expression of CD105 is maintained at 30% or more, usually 40% or more, preferably 50% or more, and more preferably 70% or more, before passage. [Item 1-2-3-13AA] The cell according to item 1-2-3-13, wherein the properties of the cell are improved (for example, the ability to differentiate into cartilage is enhanced). [Item 1-2-3-13AB] A cell population containing about 50% or more, about 60% or more, about 70% or more, about 80% or more, about 90% or more, or more, of the cells according to any one of items 1-2-3-13 to 1-13AA. [Item 1-2-3-14] A pharmaceutical product comprising cells described in any one of the above items. [Item 1-2-3-15] A method for differentiating cells, comprising the step of culturing cells obtained using a culture medium described in any one of the above items under conditions that induce differentiation. [Item 1-2-3-15A] The method according to any one of the above items, wherein the culture comprises culturing the cells after proliferation using a different differentiation medium while confirming their differentiation ability.[Item 1-2-3-15B] The method according to any one of the above items, wherein the conditions for inducing the differentiation also include differentiation conditions that are commonly used for MSCs. [Item 1-2-3-15C] The method according to any one of the above items, wherein the cells are less likely to become cartilage and / or have high cartilage differentiation potential. [Item 1-2-3-16] Differentiated cells obtained by differentiating stem cells by the method according to any one of the above items. [Item 1-2-3-17] A method for treating or preventing a subject, comprising the step of administering an effective amount of the cells or the pharmaceutical described in any one of the above items to a subject in need thereof. [Item 1-2-3-18] A method for quality control of stem cells, comprising determining whether they have a coefficient C correlated with the culture medium described in any one of the above items. [Item 1-2-3-18A] The method according to any one of the above items, wherein the coefficient C, in the case of cells, includes a coefficient relating to a cell marker. [Item 1-2-3-18B] The method according to any of the above items, wherein the cell marker includes a general marker, such as a cell marker associated with proliferation. [Item 1-2-3-18C] The method according to any of the above items, wherein the cell marker includes a marker described herein, for example, one that enhances differentiation ability or one that exhibits long-term expression. [Item 1-2-3-18D] The method according to any of the above items, wherein, in the case of a culture medium, the marker includes information relating to the identification of the component itself. [Item 1-2-3-19] A system for culturing cells, comprising: 1) a cell culture section for containing cells intended for culture; 2) a medium supply section for providing a culture medium or raw materials according to any of the above items; and 3) a cell culture condition adjustment section for adjusting culture conditions as necessary. [Item 1-2-3-20] A culture medium suitable for transporting cells containing the culture medium components described in any of the above items.[Item 1-2-3-21] A method for managing cell culture for cells suitable for cultivation using the culture medium described in any one of the above items, comprising: 1) providing information about the culture medium with respect to cells provided by the user; 2) assigning identifiable labels to the cells and the culture medium; and 3) managing whether the cells and the culture medium are compatible by referring to the labels.

[0055] [Item 1-2-3-22] Use of (1), (2) and (3) as described in Item 1-2-3-1 for culturing cells (preferably mesenchymal stem cells). [Item 1-2-3-23] Use of Item 1-2-3-22 for culturing cells (preferably mesenchymal stem cells), comprising the features of any one of Items 1-2-3-2 to 1-2-3-6C. [Item 1-2-3-24] A method for maintaining or improving the quality of cells (preferably mesenchymal stem cells) (preferably, the expression level of at least one selected from the group consisting of CD44, CD73, CD90 and CD105, and / or differentiation potential after long-term passage), comprising the step of contacting the cells in a culture medium containing the components described in Item 1-2-3-1. [Item 1-2-3-25] The method according to Item 1-2-3-24, comprising the features of any one of Items 1-2-3-2 to 1-2-3-6C. [Item 1-2-3-26] Cells or cell populations described in any one of items 1-2-3-13 to 1-2-3-13AB, or differentiated cells or cell populations described in item 1-2-3-16, for use in treatment. [Item 1-2-3-27] Cells or cell populations described in any one of items 1-2-3-13 to 1-2-3-13AB, or differentiated cells or cell populations described in item 1-2-3-16, for use in regenerative medicine or the treatment of cartilage diseases. [Item 1-2-3-28] Use of cells or cell populations described in any one of items 1-2-3-13 to 1-2-3-13AB, or differentiated cells or cell populations described in item 1-2-3-16, in the manufacture of drugs for regenerative medicine or the treatment of cartilage diseases. [Item 1-2-3-29] A method for performing regenerative medicine or treating cartilage disease in a subject in need thereof, comprising the step of administering to the subject an effective amount of cells or cell populations described in any one of items 1-2-3-13 to 1-2-3-13AB, or differentiated cells or cell populations described in item 1-2-3-16.

[0056] Examples including Form 1-2-4 (1), (2), and (4): [Item 1-2-4-1] A culture medium including (1), (2), and (4). [Item 1-2-4-2] A culture medium according to any of the above items, including (1'), (2'), and (4'). [Item 1-2-4-3] A culture medium according to any one of the above items, including (1"), (2"), and (4"). [Item 1-2-4-4] A culture medium according to any one of the above items, including (1A), (2A), and (4A). [Item 1-2-4-5] A culture medium according to any one of the above items, including (1B), (2B), and (4B). [Item 1-2-4-5A] A culture medium according to any one of the above items, including one or more of (1), (1'), (1"), (1A), and (1B), one or more of (2), (2'), (2"), (2A), and (2B), and one or more of (4), (4'), (4"), (4A), and (4B). [Item 1-2-4-6] A culture medium according to any one of the above items, for culturing various stem cells such as mesenchymal stem cells. [Item 1-2-4-6A] A culture medium according to any one of the above items, for culturing mesenchymal stem cells. [Item 1-2-4-6B] A serum-free culture medium according to any one of the above items. [Item 1-2-4-6C] A culture medium according to any one of the above items, which does not contain heterologous raw materials. [Item 1-2-4-7] A method for producing cells, comprising the step of culturing cells using the culture medium according to any one of the above items. [Item 1-2-4-8] The method according to any one of the above items, wherein the culturing step comprises culturing under conditions that satisfy at least one selected from the group consisting of a specific range of cell numbers, a specific container, and a specific temperature. [Item 1-2-4-8A] The range of cell numbers is such that MSCs are not seeded at low densities, preferably 500 cells / cm². ２ ~20000 cells / cm ２ That's good enough, and more preferably, 500-1000 / cm ２ ~10,000 / cm ２ For example, 2000 cells / cm² ２The method according to any one of the above items, although often seeded before or after. [Item 1-2-4-8B] The method according to any one of the above items, wherein the range of the cell number is a specific value such as 1500 cells / well at the time of screening, and / or is carried out under conditions such as 4500 cells / well which are estimated to be confluent. [Item 1-2-4-8C] The method according to any one of the above items, wherein the specific container is a container of a suitable material (including with or without coating). [Item 1-2-4-8D] The method according to any one of the above items, wherein the specific temperature is a suitable temperature (such as 25-37°C or 30°C). [Item 1-2-4-9] A cell culture apparatus comprising a culture medium according to any one of the above items or means for providing the culture medium, a container capable of containing the culture medium, and means for providing conditions for culturing cells as necessary. [Item 1-2-4-10] A method for preserving cells, comprising the step of preserving cells using the culture medium according to any one of the above items. [Item 1-2-4-11] A composition for preserving cells, comprising the component described in any one of the above items. [Item 1-2-4-12] A cell-containing composition comprising the component described in any one of the above items and cells. [Item 1-2-4-13] Cells cultured using the component described in any one of the above items. [Item 1-2-4-13A] The cells described in any one of the above items, wherein the expression of at least one selected from the group consisting of CD44, CD73, CD90, and CD105 is not significantly reduced. [Item 1-2-4-13B] A cell population comprising the cells described in any of the above items, wherein the expression of at least one selected from the group consisting of CD44, CD73, CD90, and CD105 is not significantly reduced even after long-term passage. [Item 1-2-4-13C] The marker is at least one of CD44, CD73, CD90, and CD105, and at least one of these does not decrease. Typically, CD44 does not decrease, but the others often decrease, and it is a desirable and advantageous characteristic that CD73 and CD90, or CD44, CD90 and CD105, do not decrease, in any one of the above items, a cell or cell population.[Item 1-2-4-13D] Cells or cell populations according to any one of the above items, in which high expression of CD90 is maintained. [Item 1-2-4-13E] Cells or cell populations according to any one of the above items, in which the expression of at least one of the markers (preferably at least one of CD73, CD90, and CD105) is not significantly reduced when the number of passages is 5 or more, preferably 6 or more, and more preferably 7 or more. [Item 1-2-4-13F] Cells or cell populations according to any one of the above items, in which the expression of at least one of the markers (preferably at least one of CD73, CD90, and CD105) is not significantly reduced when the number of passages is 8 or more. [Item 1-2-4-13G] Cells or cell populations according to any one of the above items, in which differentiation ability is maintained when the number of passages is 5 or more, preferably 6 or more, and more preferably 7 or more. [Item 1-2-4-13H] Cells or cell populations according to any one of the above items, in which differentiation ability is maintained when the number of passages is 8 or more. [Item 1-2-4-13I] A cell or cell population according to any one of the above items, wherein the expression of CD90, CD44, and CD73 is maintained at 70% or more, usually 80% or more, preferably 90% or more, and more preferably 95% or more, respectively, before passage. [Item 1-2-4-13J] A cell or cell population according to any one of the above items, wherein the expression of CD105 is maintained at 30% or more, usually 40% or more, preferably 50% or more, and more preferably 70% or more, before passage. [Item 1-2-4-13AA] The cell according to item 1-2-4-13, wherein the properties of the cell are improved (for example, the ability to differentiate into cartilage is enhanced). [Item 1-2-4-13AB] A cell population containing about 50% or more, about 60% or more, about 70% or more, about 80% or more, about 90% or more, or more, of the cells according to any one of items 1-2-4-13 to 1-13AA. [Item 1-2-4-14] A pharmaceutical product comprising cells described in any one of the above items. [Item 1-2-4-15] A method for differentiating cells, comprising the step of culturing cells obtained using a culture medium described in any one of the above items under conditions that induce differentiation. [Item 1-2-4-15A] The method according to any one of the above items, wherein the culture comprises culturing the cells after proliferation using a different differentiation medium while confirming their differentiation ability.[Item 1-2-4-15B] The method according to any one of the above items, wherein the conditions under which the differentiation is induced also include differentiation conditions that are commonly used for MSCs. [Item 1-2-4-15C] The method according to any one of the above items, wherein the cells are less likely to become cartilage and / or have high cartilage differentiation potential. [Item 1-2-4-16] Differentiated cells obtained by differentiating stem cells by the method according to any one of the above items. [Item 1-2-4-17] A method for treating or preventing a subject, comprising the step of administering an effective amount of the cells or the pharmaceutical described in any one of the above items to a subject in need thereof. [Item 1-2-4-18] A method for quality control of stem cells, comprising determining whether they have a coefficient C correlated with the culture medium described in any one of the above items. [Item 1-2-4-18A] The method according to any one of the above items, wherein the coefficient C, in the case of cells, includes a coefficient relating to a cell marker. [Item 1-2-4-18B] The method according to any of the above items, wherein the cell marker includes a general marker, such as a cell marker associated with proliferation. [Item 1-2-4-18C] The method according to any of the above items, wherein the cell marker includes a marker described herein, for example, one that enhances differentiation ability or one that exhibits long-term expression. [Item 1-2-4-18D] The method according to any of the above items, wherein, in the case of a culture medium, the marker includes information relating to the identification of the component itself. [Item 1-2-4-19] A system for culturing cells, comprising: 1) a cell culture section for containing cells intended for culture; 2) a medium supply section for providing a culture medium or raw materials according to any of the above items; and 3) a cell culture condition adjustment section for adjusting culture conditions as necessary. [Item 1-2-4-20] A culture medium suitable for transporting cells containing the culture medium components described in any of the above items.[Item 1-2-4-21] A method for managing cell culture for cells suitable for cultivation using the culture medium described in any one of the above items, comprising: 1) providing information about the culture medium with respect to cells provided by the user; 2) assigning identifiable labels to the cells and the culture medium; and 3) managing whether the cells and the culture medium are compatible by referring to the labels.

[0057] [Item 1-2-4-22] The use of (1), (2) and (4) described in Item 1-2-4-1 for culturing cells (preferably mesenchymal stem cells). [Item 1-2-4-23] The use of the cell (preferably mesenchymal stem cells) described in Item 1-2-4-22, comprising the features of any one of Items 1-2-4-2 to 1-2-4-6C. [Item 1-2-4-24] A method for maintaining or improving the quality of cells (preferably mesenchymal stem cells) (preferably, the expression level of at least one selected from the group consisting of CD44, CD73, CD90 and CD105, and / or differentiation potential after long-term passage), comprising the step of contacting the cells in a culture medium comprising the components described in Item 1-2-4-1. [Item 1-2-4-25] The method according to Item 1-2-4-24, comprising the features of any one of Items 1-2-4-2 to 1-2-4-6C. [Item 1-2-4-26] Cells or cell populations described in any one of items 1-2-4-13 to 1-2-4-13AB, or differentiated cells or cell populations described in item 1-2-4-16, for use in treatment. [Item 1-2-4-27] Cells or cell populations described in any one of items 1-2-4-13 to 1-2-4-13AB, or differentiated cells or cell populations described in item 1-2-4-16, for use in regenerative medicine or in the treatment of cartilage diseases. [Item 1-2-4-28] Use of cells or cell populations described in any one of items 1-2-4-13 to 1-2-4-13AB, or differentiated cells or cell populations described in item 1-2-4-16, in the manufacture of drugs for regenerative medicine or in the treatment of cartilage diseases. [Item 1-2-4-29] A method for performing regenerative medicine or treating cartilage disease in a subject in need thereof, comprising the step of administering to the subject an effective amount of cells or cell populations described in any one of items 1-2-4-13 to 1-2-4-13AB, or differentiated cells or cell populations described in item 1-2-4-16.

[0058] Examples including Forms 1-2-5 (1), (2), and (5): [Item 1-2-5-1] A culture medium including (1), (2), and (5). [Item 1-2-5-2] A culture medium according to any of the above items, including (1'), (2'), and (5'). [Item 1-2-5-3] A culture medium according to any one of the above items, including (1"), (2"), and (5"). [Item 1-2-5-4] A culture medium according to any one of the above items, including (1A), (2A), and (5A). [Item 1-2-5-5] A culture medium according to any one of the above items, including (1B), (2B), and (5B). [Item 1-2-5-5A] A culture medium according to any one of the above items, including one or more of (1), (1'), (1"), (1A), and (1B), one or more of (2), (2'), (2"), (2A), and (2B), and one or more of (5), (5'), (5"), (5A), and (5B). [Item 1-2-5-6] A culture medium according to any one of the above items, for culturing various stem cells such as mesenchymal stem cells. [Item 1-2-5-6A] A culture medium according to any one of the above items, for culturing mesenchymal stem cells. [Item 1-2-5-6B] A serum-free culture medium according to any one of the above items. [Item 1-2-5-6C] A culture medium according to any one of the above items, which does not contain heterologous raw materials. [Item 1-2-5-7] A method for producing cells, comprising the step of culturing cells using the culture medium according to any one of the above items. [Item 1-2-5-8] The method according to any one of the above items, wherein the culturing step comprises culturing under conditions that satisfy at least one selected from the group consisting of a specific range of cell numbers, a specific container, and a specific temperature. [Item 1-2-5-8A] The range of cell numbers is such that MSCs are not seeded at low densities, preferably 500 cells / cm². ２ ~20000 cells / cm ２ That's good enough, and more preferably, 500-1000 / cm ２ ~10,000 / cm ２ For example, 2000 cells / cm² ２The method according to any one of the above items, although often seeded before or after. [Item 1-2-5-8B] The method according to any one of the above items, wherein the range of the cell number is a specific value such as 1500 cells / well at the time of screening, and / or is carried out under conditions such as 4500 cells / well which are estimated to be confluent. [Item 1-2-5-8C] The method according to any one of the above items, wherein the specific container is a container of a suitable material (including with or without coating). [Item 1-2-5-8D] The method according to any one of the above items, wherein the specific temperature is a suitable temperature (such as 25-37°C or 30°C). [Item 1-2-5-9] A cell culture apparatus comprising a culture medium according to any one of the above items or means for providing the culture medium, a container capable of containing the culture medium, and means for providing conditions for culturing cells as necessary. [Item 1-2-5-10] A method for preserving cells, comprising the step of preserving cells using the culture medium according to any one of the above items. [Item 1-2-5-11] A composition for preserving cells, comprising the component described in any one of the above items. [Item 1-2-5-12] A cell-containing composition comprising the component described in any one of the above items and cells. [Item 1-2-5-13] Cells cultured using the component described in any one of the above items. [Item 1-2-5-13A] The cells described in any one of the above items, wherein the expression of at least one selected from the group consisting of CD44, CD73, CD90, and CD105 is not significantly reduced. [Item 1-2-5-13B] A cell population comprising the cells described in any of the above items, wherein the expression of at least one selected from the group consisting of CD44, CD73, CD90, and CD105 is not significantly reduced even after long-term passage. [Item 1-2-5-13C] The marker is at least one of CD44, CD73, CD90, and CD105, and at least one of these does not decrease. Typically, CD44 does not decrease, but the others often decrease, and it is a desirable and advantageous characteristic that CD73 and CD90, or CD44, CD90 and CD105, do not decrease, in any one of the above items, a cell or cell population.[Item 1-2-5-13D] Cells or cell populations according to any one of the above items, in which high expression of CD90 is maintained. [Item 1-2-5-13E] Cells or cell populations according to any one of the above items, in which the expression of at least one of the markers (preferably at least one of CD73, CD90, and CD105) is not significantly reduced when the number of passages is 5 or more, preferably 6 or more, and more preferably 7 or more. [Item 1-2-5-13F] Cells or cell populations according to any one of the above items, in which the expression of at least one of the markers (preferably at least one of CD73, CD90, and CD105) is not significantly reduced when the number of passages is 8 or more. [Item 1-2-5-13G] Cells or cell populations according to any one of the above items, in which differentiation ability is maintained when the number of passages is 5 or more, preferably 6 or more, and more preferably 7 or more. [Item 1-2-5-13H] Cells or cell populations according to any one of the above items, in which differentiation ability is maintained when the number of passages is 8 or more. [Item 1-2-5-13I] A cell or cell population according to any one of the above items, wherein the expression of CD90, CD44, and CD73 is maintained at 70% or more, usually 80% or more, preferably 90% or more, and more preferably 95% or more, respectively, before passage. [Item 1-2-5-13J] A cell or cell population according to any one of the above items, wherein the expression of CD105 is maintained at 30% or more, usually 40% or more, preferably 50% or more, and more preferably 70% or more, before passage. [Item 1-2-5-13AA] The cell according to item 1-2-5-13, wherein the properties of the cell are improved (for example, the ability to differentiate into cartilage is enhanced). [Item 1-2-5-13AB] A cell population containing about 50% or more, about 60% or more, about 70% or more, about 80% or more, about 90% or more, or more, of the cells according to any one of items 1-2-5-13 to 1-13AA. [Item 1-2-5-14] A pharmaceutical product comprising cells described in any one of the above items. [Item 1-2-5-15] A method for differentiating cells, comprising the step of culturing cells obtained using a culture medium described in any one of the above items under conditions that induce differentiation. [Item 1-2-5-15A] The method according to any one of the above items, wherein the culture comprises culturing the cells after proliferation using a different differentiation medium while confirming their differentiation ability.[Item 1-2-5-15B] The method according to any one of the above items, wherein the conditions under which the differentiation is induced also include differentiation conditions that are commonly used for MSCs. [Item 1-2-5-15C] The method according to any one of the above items, wherein the cells are less likely to become cartilage and / or have high cartilage differentiation potential. [Item 1-2-5-16] Differentiated cells obtained by differentiating stem cells by the method according to any one of the above items. [Item 1-2-5-17] A method for treating or preventing a subject, comprising the step of administering an effective amount of the cells or the pharmaceutical described in any one of the above items to a subject in need thereof. [Item 1-2-5-18] A method for quality control of stem cells, comprising determining whether they have a coefficient C correlated with the culture medium described in any one of the above items. [Item 1-2-5-18A] The method according to any one of the above items, wherein the coefficient C, in the case of cells, includes a coefficient relating to a cell marker. [Item 1-2-5-18B] The method according to any of the above items, wherein the cell marker includes a general marker such as a cell marker associated with proliferation. [Item 1-2-5-18C] The method according to any of the above items, wherein the cell marker includes a marker described herein, for example, one that enhances differentiation ability or one that exhibits long-term expression. [Item 1-2-5-18D] The method according to any of the above items, wherein, in the case of a culture medium, the marker includes information relating to the identification of the component itself. [Item 1-2-5-19] A system for culturing cells, comprising: 1) a cell culture section for containing cells intended for culture; 2) a medium supply section for providing a culture medium or raw materials according to any of the above items; and 3) a cell culture condition adjustment section for adjusting culture conditions as necessary. [Item 1-2-5-20] A culture medium suitable for transporting cells containing the culture medium components described in any of the above items.[Item 1-2-5-21] A method for managing cell culture for cells suitable for cultivation using the culture medium described in any one of the above items, comprising: 1) providing information about the culture medium with respect to cells provided by the user; 2) assigning identifiable labels to the cells and the culture medium; and 3) managing whether the cells and the culture medium are compatible by referring to the labels.

[0059] [Item 1-2-5-22] The use of (1), (2) and (5) described in Item 1-2-5-1 for culturing cells (preferably mesenchymal stem cells). [Item 1-2-5-23] The use of the cell culture method described in Item 1-2-5-22, comprising the features of any one of Items 1-2-5-2 to 1-2-5-6C. [Item 1-2-5-24] A method for maintaining or improving the quality of cells (preferably mesenchymal stem cells) (preferably, the expression level of at least one selected from the group consisting of CD44, CD73, CD90 and CD105, and / or differentiation potential after long-term passage), comprising the step of contacting the cells in a culture medium containing the components described in Item 1-2-5-1. [Item 1-2-5-25] The method according to Item 1-2-5-24, comprising the features of any one of Items 1-2-5-2 to 1-2-5-6C. [Item 1-2-5-26] Cells or cell populations described in any one of items 1-2-5-13 to 1-2-5-13AB, or differentiated cells or cell populations described in item 1-2-5-16, for use in treatment. [Item 1-2-5-27] Cells or cell populations described in any one of items 1-2-5-13 to 1-2-5-13AB, or differentiated cells or cell populations described in item 1-2-5-16, for use in regenerative medicine or in the treatment of cartilage diseases. [Item 1-2-5-28] Use of cells or cell populations described in any one of items 1-2-5-13 to 1-2-5-13AB, or differentiated cells or cell populations described in item 1-2-5-16, in the manufacture of drugs for regenerative medicine or in the treatment of cartilage diseases. [Item 1-2-5-29] A method for performing regenerative medicine or treating cartilage disease in a subject in need thereof, comprising the step of administering to the subject an effective amount of cells or cell populations described in any one of items 1-2-5-13 to 1-2-5-13AB, or differentiated cells or cell populations described in item 1-2-5-16.

[0060] Examples including Forms 1-2-6 (1), (2), and (6): [Item 1-2-6-1] A culture medium including (1), (2), and (6). [Item 1-2-6-2] A culture medium according to any of the above items, including (1'), (2'), and (6'). [Item 1-2-6-3] A culture medium according to any one of the above items, including (1"), (2"), and (6"). [Item 1-2-6-4] A culture medium according to any one of the above items, including (1A), (2A), and (6A). [Item 1-2-6-5] A culture medium according to any one of the above items, including (1B), (2B), and (6B). [Item 1-2-6-5A] A culture medium according to any one of the above items, including one or more of (1), (1'), (1"), (1A), and (1B), one or more of (2), (2'), (2"), (2A), and (2B), and one or more of (6), (6'), (6"), (6A), and (6B). [Item 1-2-6-6] A culture medium according to any one of the above items, for culturing various stem cells such as mesenchymal stem cells. [Item 1-2-6-6A] A culture medium according to any one of the above items, for culturing mesenchymal stem cells. [Item 1-2-6-6B] A serum-free culture medium according to any one of the above items. [Item 1-2-6-6C] A culture medium according to any one of the above items, which does not contain heterologous raw materials. [Item 1-2-6-7] A method for producing cells, comprising the step of culturing cells using the culture medium according to any one of the above items. [Item 1-2-6-8] The method according to any one of the above items, wherein the culturing step comprises culturing under conditions that satisfy at least one selected from the group consisting of a specific range of cell numbers, a specific container, and a specific temperature. [Item 1-2-6-8A] The range of cell numbers is such that MSCs are not seeded at low densities, preferably 500 cells / cm². ２ ~20000 cells / cm ２ That's good enough, and more preferably, 500-1000 / cm ２ ~10,000 / cm ２ For example, 2000 cells / cm² ２The method according to any one of the above items, although often seeded before or after. [Item 1-2-6-8B] The method according to any one of the above items, wherein the range of the cell number is a specific value, such as 1500 cells / well, at the time of screening, and / or is carried out under conditions such as 4500 cells / well, which are estimated to be confluent. [Item 1-2-6-8C] The method according to any one of the above items, wherein the specific container is a container of a suitable material (including with or without coating). [Item 1-2-6-8D] The method according to any one of the above items, wherein the specific temperature is a suitable temperature (such as 25-37°C or 30°C). [Item 1-2-6-9] A cell culture apparatus comprising a culture medium according to any one of the above items or means for providing the culture medium, a container capable of containing the culture medium, and means for providing conditions for culturing cells as necessary. [Item 1-2-6-10] A method for preserving cells, comprising the step of preserving cells using the culture medium according to any one of the above items. [Item 1-2-6-11] A composition for preserving cells, comprising the component described in any one of the above items. [Item 1-2-6-12] A cell-containing composition comprising the component described in any one of the above items and cells. [Item 1-2-6-13] Cells cultured using the component described in any one of the above items. [Item 1-2-6-13A] The cells described in any one of the above items, wherein the expression of at least one selected from the group consisting of CD44, CD73, CD90, and CD105 is not significantly reduced. [Item 1-2-6-13B] A cell population comprising the cells described in any of the above items, wherein the expression of at least one selected from the group consisting of CD44, CD73, CD90, and CD105 is not significantly reduced even after long-term passage. [Item 1-2-6-13C] The marker is at least one of CD44, CD73, CD90, and CD105, and at least one of these does not decrease. Typically, CD44 does not decrease, but the others often decrease, and it is a desirable and advantageous characteristic that CD73 and CD90, or CD44, CD90 and CD105, do not decrease, in any one of the above items, a cell or cell population.[Item 1-2-6-13D] Cells or cell populations according to any one of the above items, in which high expression of CD90 is maintained. [Item 1-2-6-13E] Cells or cell populations according to any one of the above items, in which the expression of at least one of the markers (preferably at least one of CD73, CD90, and CD105) is not significantly reduced when the number of passages is 5 or more, preferably 6 or more, and more preferably 7 or more. [Item 1-2-6-13F] Cells or cell populations according to any one of the above items, in which the expression of at least one of the markers (preferably at least one of CD73, CD90, and CD105) is not significantly reduced when the number of passages is 8 or more. [Item 1-2-6-13G] Cells or cell populations according to any one of the above items, in which differentiation ability is maintained when the number of passages is 5 or more, preferably 6 or more, and more preferably 7 or more. [Item 1-2-6-13H] Cells or cell populations according to any one of the above items, in which differentiation ability is maintained when the number of passages is 8 or more. [Item 1-2-6-13I] A cell or cell population according to any one of the above items, wherein the expression of CD90, CD44, and CD73 is maintained at 70% or more, usually 80% or more, preferably 90% or more, and more preferably 95% or more, respectively, before passage. [Item 1-2-6-13J] A cell or cell population according to any one of the above items, wherein the expression of CD105 is maintained at 30% or more, usually 40% or more, preferably 50% or more, and more preferably 70% or more, before passage. [Item 1-2-6-13AA] The cell according to item 1-2-6-13, wherein the properties of the cell are improved (for example, the ability to differentiate into cartilage is enhanced). [Item 1-2-6-13AB] A cell population containing about 50% or more, about 60% or more, about 70% or more, about 80% or more, about 90% or more, or more, of the cells according to any one of items 1-2-6-13 to 1-13AA. [Item 1-2-6-14] A pharmaceutical product comprising cells described in any one of the above items. [Item 1-2-6-15] A method for differentiating cells, comprising the step of culturing cells obtained using a culture medium described in any one of the above items under conditions that induce differentiation. [Item 1-2-6-15A] The method according to any one of the above items, wherein the culture comprises culturing the cells after proliferation using a different differentiation medium while confirming their differentiation ability.[Item 1-2-6-15B] The method according to any one of the above items, wherein the conditions under which the differentiation is induced also include differentiation conditions that are commonly used for MSCs. [Item 1-2-6-15C] The method according to any one of the above items, wherein the cells are less likely to become cartilage and / or have high cartilage differentiation potential. [Item 1-2-6-16] Differentiated cells obtained by differentiating stem cells by the method according to any one of the above items. [Item 1-2-6-17] A method for treating or preventing a subject, comprising the step of administering an effective amount of the cells or the pharmaceutical described in any one of the above items to a subject in need thereof. [Item 1-2-6-18] A method for quality control of stem cells, comprising determining whether they have a coefficient C correlated with the culture medium described in any one of the above items. [Item 1-2-6-18A] The method according to any one of the above items, wherein the coefficient C, in the case of cells, includes a coefficient relating to a cell marker. [Item 1-2-6-18B] The method according to any of the above items, wherein the cell marker includes a general marker, such as a cell marker associated with proliferation. [Item 1-2-6-18C] The method according to any of the above items, wherein the cell marker includes a marker described herein, for example, one that enhances differentiation ability or one that exhibits long-term expression. [Item 1-2-6-18D] The method according to any of the above items, wherein, in the case of a culture medium, the marker includes information relating to the identification of the component itself. [Item 1-2-6-19] A system for culturing cells, comprising: 1) a cell culture section for containing cells intended for culture; 2) a medium supply section for providing a culture medium or raw materials according to any of the above items; and 3) a cell culture condition adjustment section for adjusting culture conditions as necessary. [Item 1-2-6-20] A culture medium suitable for transporting cells containing the culture medium components described in any of the above items.[Item 1-2-6-21] A method for managing cell culture for cells suitable for cultivation using the culture medium described in any one of the above items, comprising: 1) providing information about the culture medium with respect to cells provided by the user; 2) assigning identifiable labels to the cells and the culture medium; and 3) managing whether the cells and the culture medium are compatible by referring to the labels.

[0061] [Item 1-2-6-22] Use of (1), (2) and (6) described in Item 1-2-6-1 for culturing cells (preferably mesenchymal stem cells). [Item 1-2-6-23] Use of Item 1-2-6-22 for culturing cells (preferably mesenchymal stem cells), comprising the features of any one of Items 1-2-6-2 to 1-2-6-6C. [Item 1-2-6-24] A method for maintaining or improving the quality of cells (preferably mesenchymal stem cells) (preferably, at least one expression level selected from the group consisting of CD44, CD73, CD90 and CD105, and / or differentiation potential after long-term passage), comprising the step of contacting the cells in a culture medium containing the components described in Item 1-2-6-1. [Item 1-2-6-25] The method according to Item 1-2-6-24, comprising the features of any one of Items 1-2-6-2 to 1-2-6-6C. [Item 1-2-6-26] Cells or cell populations described in any one of items 1-2-6-13 to 1-2-6-13AB, or differentiated cells or cell populations described in item 1-2-6-16, for use in treatment. [Item 1-2-6-27] Cells or cell populations described in any one of items 1-2-6-13 to 1-2-6-13AB, or differentiated cells or cell populations described in item 1-2-6-16, for use in regenerative medicine or the treatment of cartilage diseases. [Item 1-2-6-28] Use of cells or cell populations described in any one of items 1-2-6-13 to 1-2-6-13AB, or differentiated cells or cell populations described in item 1-2-6-16, in the manufacture of drugs for regenerative medicine or the treatment of cartilage diseases. [Item 1-2-6-29] A method for performing regenerative medicine or treating cartilage disease in a subject in need thereof, comprising the step of administering to the subject an effective amount of cells or cell populations described in any one of items 1-2-6-13 to 1-2-6-13AB, or differentiated cells or cell populations described in item 1-2-6-16.

[0062] Examples including Forms 1-2-7 (1), (2), and (7): [Item 1-2-7-1] A culture medium including (1), (2), and (7). [Item 1-2-7-2] A culture medium according to any of the above items, including (1'), (2'), and (7'). [Item 1-2-7-3] A culture medium according to any one of the above items, including (1"), (2"), and (7"). [Item 1-2-7-4] A culture medium according to any one of the above items, including (1A), (2A), and (7A). [Item 1-2-7-5] A culture medium according to any one of the above items, including (1B), (2B), and (7B). [Item 1-2-7-5A] A culture medium according to any one of the above items, including one or more of (1), (1'), (1"), (1A), and (1B), one or more of (2), (2'), (2"), (2A), and (2B), and one or more of (7), (7'), (7"), (7A), and (7B). [Item 1-2-7-6] A culture medium according to any one of the above items, for culturing various stem cells such as mesenchymal stem cells. [Item 1-2-7-6A] A culture medium according to any one of the above items, for culturing mesenchymal stem cells. [Item 1-2-7-6B] A serum-free culture medium according to any one of the above items. [Item 1-2-7-6C] A culture medium according to any one of the above items, which does not contain heterologous raw materials. [Item 1-2-7-7] A method for producing cells, comprising the step of culturing cells using the culture medium according to any one of the above items. [Item 1-2-7-8] The method according to any one of the above items, wherein the culturing step comprises culturing under conditions that satisfy at least one selected from the group consisting of a specific range of cell numbers, a specific container, and a specific temperature. [Item 1-2-7-8A] The range of cell numbers is such that MSCs are not seeded at low densities, preferably 500 cells / cm². ２ ~20000 cells / cm ２ That's good enough, and more preferably, 500-1000 / cm ２ ~10,000 / cm ２ For example, 2000 cells / cm² ２The method according to any one of the above items, although often seeded before or after. [Item 1-2-7-8B] The method according to any one of the above items, wherein the range of the cell number is a specific value such as 1500 cells / well at the time of screening, and / or is carried out under conditions such as 4500 cells / well which are estimated to become confluent. [Item 1-2-7-8C] The method according to any one of the above items, wherein the specific container is a container of a suitable material (including with or without coating). [Item 1-2-7-8D] The method according to any one of the above items, wherein the specific temperature is a suitable temperature (25-37°C, or 30°C, etc.). [Item 1-2-7-9] A cell culture apparatus comprising a culture medium according to any one of the above items or means for providing the culture medium, a container capable of containing the culture medium, and means for providing conditions for culturing cells as necessary. [Item 1-2-7-10] A method for preserving cells, comprising the step of preserving cells using the culture medium according to any one of the above items. [Item 1-2-7-11] A composition for preserving cells, comprising the component described in any one of the above items. [Item 1-2-7-12] A cell-containing composition comprising the component described in any one of the above items and cells. [Item 1-2-7-13] Cells cultured using the component described in any one of the above items. [Item 1-2-7-13A] The cells described in any one of the above items, wherein the expression of at least one selected from the group consisting of CD44, CD73, CD90, and CD105 is not significantly reduced. [Item 1-2-7-13B] A cell population comprising the cells described in any of the above items, wherein the expression of at least one selected from the group consisting of CD44, CD73, CD90, and CD105 is not significantly reduced even after long-term passage. [Item 1-2-7-13C] The marker is at least one of CD44, CD73, CD90, and CD105, and at least one of these does not decrease. Typically, CD44 does not decrease, but the others often decrease, and it is a desirable and advantageous characteristic that CD73 and CD90, or CD44, CD90 and CD105, do not decrease, in any one of the above items, a cell or cell population.[Item 1-2-7-13D] Cells or cell populations according to any one of the above items, in which high expression of CD90 is maintained. [Item 1-2-7-13E] Cells or cell populations according to any one of the above items, in which the expression of at least one of the markers (preferably at least one of CD73, CD90, and CD105) is not significantly reduced when the number of passages is 5 or more, preferably 6 or more, and more preferably 7 or more. [Item 1-2-7-13F] Cells or cell populations according to any one of the above items, in which the expression of at least one of the markers (preferably at least one of CD73, CD90, and CD105) is not significantly reduced when the number of passages is 8 or more. [Item 1-2-7-13G] Cells or cell populations according to any one of the above items, in which differentiation ability is maintained when the number of passages is 5 or more, preferably 6 or more, and more preferably 7 or more. [Item 1-2-7-13H] Cells or cell populations according to any one of the above items, in which differentiation ability is maintained when the number of passages is 8 or more. [Item 1-2-7-13I] A cell or cell population according to any one of the above items, wherein the expression of CD90, CD44, and CD73 is maintained at 70% or more, usually 80% or more, preferably 90% or more, and more preferably 95% or more, respectively, before passage. [Item 1-2-7-13J] A cell or cell population according to any one of the above items, wherein the expression of CD105 is maintained at 30% or more, usually 40% or more, preferably 50% or more, and more preferably 70% or more, before passage. [Item 1-2-7-13AA] The cell according to item 1-2-7-13, wherein the properties of the cell are improved (for example, the ability to differentiate into cartilage is enhanced). [Item 1-2-7-13AB] A cell population containing about 50% or more, about 60% or more, about 70% or more, about 80% or more, about 90% or more, or more, of the cells according to any one of items 1-2-7-13 to 1-13AA. [Item 1-2-7-14] A pharmaceutical product comprising cells described in any one of the above items. [Item 1-2-7-15] A method for differentiating cells, comprising the step of culturing cells obtained using a culture medium described in any one of the above items under conditions that induce differentiation. [Item 1-2-7-15A] The method according to any one of the above items, wherein the culture comprises culturing the cells after proliferation using a different differentiation medium while confirming their differentiation ability.[Item 1-2-7-15B] The method according to any one of the above items, wherein the conditions under which the differentiation is induced also include differentiation conditions that are commonly used for MSCs. [Item 1-2-7-15C] The method according to any one of the above items, wherein the cells are less likely to become cartilage and / or have high cartilage differentiation potential. [Item 1-2-7-16] Differentiated cells obtained by differentiating stem cells by the method according to any one of the above items. [Item 1-2-7-17] A method for treating or preventing a subject, comprising the step of administering an effective amount of the cells or the pharmaceutical described in any one of the above items to a subject in need thereof. [Item 1-2-7-18] A method for quality control of stem cells, comprising determining whether they have a coefficient C correlated with the culture medium described in any one of the above items. [Item 1-2-7-18A] The method according to any one of the above items, wherein the coefficient C, in the case of cells, includes a coefficient relating to a cell marker. [Item 1-2-7-18B] The method according to any of the above items, wherein the cell marker includes a general marker, such as a cell marker associated with proliferation. [Item 1-2-7-18C] The method according to any of the above items, wherein the cell marker includes a marker described herein, for example, one that enhances differentiation ability or one that exhibits long-term expression. [Item 1-2-7-18D] The method according to any of the above items, wherein, in the case of a culture medium, the marker includes information relating to the identification of the component itself. [Item 1-2-7-19] A system for culturing cells, comprising: 1) a cell culture section for containing cells intended for culture; 2) a medium supply section for providing a culture medium or its raw materials as described in any of the above items; and 3) a cell culture condition adjustment section for adjusting culture conditions as necessary. [Item 1-2-7-20] A culture medium suitable for transporting cells containing the culture medium components described in any of the above items.[Item 1-2-7-21] A method for managing cell culture for cells suitable for cultivation using the culture medium described in any one of the above items, comprising: 1) providing information about the culture medium with respect to cells provided by the user; 2) assigning identifiable labels to the cells and the culture medium; and 3) managing whether the cells and the culture medium are compatible by referring to the labels.

[0063] [Item 1-2-7-22] The use of (1), (2) and (7) described in Item 1-2-7-1 for culturing cells (preferably mesenchymal stem cells). [Item 1-2-7-23] The use of the cell (preferably mesenchymal stem cells) described in Item 1-2-7-22, comprising the features of any one of Items 1-2-7-2 to 1-2-7-6C. [Item 1-2-7-24] A method for maintaining or improving the quality of cells (preferably mesenchymal stem cells) (preferably, the expression level of at least one selected from the group consisting of CD44, CD73, CD90 and CD105, and / or differentiation potential after long-term passage), comprising the step of contacting the cells in a culture medium comprising the components described in Item 1-2-7-1. [Item 1-2-7-25] The method according to Item 1-2-7-24, comprising the features of any one of Items 1-2-7-2 to 1-2-7-6C. [Item 1-2-7-26] Cells or cell populations described in any one of items 1-2-7-13 to 1-2-7-13AB, or differentiated cells or cell populations described in item 1-2-7-16, for use in treatment. [Item 1-2-7-27] Cells or cell populations described in any one of items 1-2-7-13 to 1-2-7-13AB, or differentiated cells or cell populations described in item 1-2-7-16, for use in regenerative medicine or in the treatment of cartilage diseases. [Item 1-2-7-28] Use of cells or cell populations described in any one of items 1-2-7-13 to 1-2-7-13AB, or differentiated cells or cell populations described in item 1-2-7-16, in the manufacture of drugs for regenerative medicine or in the treatment of cartilage diseases. [Item 1-2-7-29] A method for performing regenerative medicine or treating cartilage disease in a subject in need thereof, comprising the step of administering to the subject an effective amount of cells or cell populations described in any one of items 1-2-7-13 to 1-2-7-13AB, or differentiated cells or cell populations described in item 1-2-7-16.

[0064] Examples including Forms 1-3-4 (1), (3), and (4): [Item 1-3-4-1] A culture medium including (1), (3), and (4). [Item 1-3-4-2] A culture medium according to any of the above items, including (1'), (3'), and (4'). [Item 1-3-4-3] A culture medium according to any one of the above items, including (1"), (3"), and (4"). [Item 1-3-4-4] A culture medium according to any one of the above items, including (1A), (3A), and (4A). [Item 1-3-4-5] A culture medium according to any one of the above items, including (1B), (3B), and (4B). [Item 1-3-4-5A] A culture medium according to any one of the above items, including one or more of (1), (1'), (1"), (1A), and (1B), one or more of (3), (3'), (3"), (3A), and (3B), and one or more of (4), (4'), (4"), (4A), and (4B). [Item 1-3-4-6] A culture medium according to any one of the above items, for culturing various stem cells such as mesenchymal stem cells. [Item 1-3-4-6A] A culture medium according to any one of the above items, for culturing mesenchymal stem cells. [Item 1-3-4-6B] A serum-free culture medium according to any one of the above items. [Item 1-3-4-6C] A culture medium according to any one of the above items, which does not contain heterologous raw materials. [Item 1-3-4-7] A method for producing cells, comprising the step of culturing cells using the culture medium according to any one of the above items. [Item 1-3-4-8] The method according to any one of the above items, wherein the culturing step comprises culturing under conditions that satisfy at least one selected from the group consisting of a specific range of cell numbers, a specific container, and a specific temperature. [Item 1-3-4-8A] The range of cell numbers is such that MSCs are not seeded at low densities, and is preferably 500 cells / cm². ２ ~20000 cells / cm ２ That's good enough, and more preferably, 500-1000 / cm ２ ~10,000 / cm ２ For example, 2000 cells / cm² ２The method according to any one of the above items, although often seeded before or after. [Item 1-3-4-8B] The method according to any one of the above items, wherein the range of the cell number is a specific value such as 1500 cells / well at the time of screening, and / or is carried out under conditions such as 4500 cells / well which are estimated to be confluent. [Item 1-3-4-8C] The method according to any one of the above items, wherein the specific container is a container of a suitable material (including with or without coating). [Item 1-3-4-8D] The method according to any one of the above items, wherein the specific temperature is a suitable temperature (35-37°C, or 30°C, etc.). [Item 1-3-4-9] A cell culture apparatus comprising a culture medium according to any one of the above items or means for providing the culture medium, a container capable of containing the culture medium, and means for providing conditions for culturing cells as necessary. [Item 1-3-4-10] A method for preserving cells, comprising the step of preserving cells using the culture medium according to any one of the above items. [Item 1-3-4-11] A composition for preserving cells, comprising the component described in any one of the above items. [Item 1-3-4-12] A cell-containing composition comprising the component described in any one of the above items and cells. [Item 1-3-4-13] Cells cultured using the component described in any one of the above items. [Item 1-3-4-13A] The cells described in any one of the above items, wherein the expression of at least one selected from the group consisting of CD44, CD73, CD90, and CD105 is not significantly reduced. [Item 1-3-4-13B] A cell population comprising the cells described in any of the above items, wherein the expression of at least one selected from the group consisting of CD44, CD73, CD90, and CD105 is not significantly reduced even after long-term passage. [Item 1-3-4-13C] The marker is at least one of CD44, CD73, CD90, and CD105, and at least one of these does not decrease. Typically, CD44 does not decrease, but the others often decrease, and it is a desirable and advantageous characteristic that CD73 and CD90, or CD44, CD90 and CD105, do not decrease, in any one of the above items, a cell or cell population.[Item 1-3-4-13D] Cells or cell populations according to any one of the above items, in which high expression of CD90 is maintained. [Item 1-3-4-13E] Cells or cell populations according to any one of the above items, in which the expression of at least one of the markers (preferably at least one of CD73, CD90, and CD105) is not significantly reduced when the number of passages is 5 or more, preferably 6 or more, and more preferably 7 or more. [Item 1-3-4-13F] Cells or cell populations according to any one of the above items, in which the expression of at least one of the markers (preferably at least one of CD73, CD90, and CD105) is not significantly reduced when the number of passages is 8 or more. [Item 1-3-4-13G] Cells or cell populations according to any one of the above items, in which differentiation ability is maintained when the number of passages is 5 or more, preferably 6 or more, and more preferably 7 or more. [Item 1-3-4-13H] Cells or cell populations according to any one of the above items, in which differentiation ability is maintained when the number of passages is 8 or more. [Item 1-3-4-13I] A cell or cell population according to any one of the above items, wherein the expression of CD90, CD44, and CD73 is maintained at 70% or more, usually 80% or more, preferably 90% or more, and more preferably 95% or more, respectively, before passage. [Item 1-3-4-13J] A cell or cell population according to any one of the above items, wherein the expression of CD105 is maintained at 30% or more, usually 40% or more, preferably 50% or more, and more preferably 70% or more, before passage. [Item 1-3-4-13AA] The cell according to item 1-3-4-13, wherein the cell properties are improved (for example, the ability to differentiate into cartilage is enhanced). [Item 1-3-4-13AB] A cell population containing about 50% or more, about 60% or more, about 70% or more, about 80% or more, about 90% or more, or more of the cells according to any one of items 1-3-4-13 to 1-13AA. [Item 1-3-4-14] A pharmaceutical product comprising cells described in any one of the above items. [Item 1-3-4-15] A method for differentiating cells, comprising the step of culturing cells obtained using a culture medium described in any one of the above items under conditions that induce differentiation. [Item 1-3-4-15A] The method according to any one of the above items, wherein the culture comprises culturing the cells after proliferation using a different differentiation medium while confirming their differentiation ability.[Item 1-3-4-15B] The method according to any one of the above items, wherein the conditions under which the differentiation is induced also include differentiation conditions that are commonly used for MSCs. [Item 1-3-4-15C] The method according to any one of the above items, wherein the cells are less likely to become cartilage and / or have high cartilage differentiation potential. [Item 1-3-4-16] Differentiated cells obtained by differentiating stem cells by the method according to any one of the above items. [Item 1-3-4-17] A method for treating or preventing a subject, comprising the step of administering an effective amount of the cells or the pharmaceutical described in any one of the above items to a subject in need thereof. [Item 1-3-4-18] A method for quality control of stem cells, comprising determining whether they have a coefficient C correlated with the culture medium described in any one of the above items. [Item 1-3-4-18A] The method according to any one of the above items, wherein the coefficient C, in the case of cells, includes a coefficient relating to a cell marker. [Item 1-3-4-18B] The method according to any of the above items, wherein the cell marker includes a general marker, such as a cell marker associated with proliferation. [Item 1-3-4-18C] The method according to any of the above items, wherein the cell marker includes a marker described herein, for example, one that enhances differentiation ability or one that exhibits long-term expression. [Item 1-3-4-18D] The method according to any of the above items, wherein, in the case of a culture medium, the marker includes information relating to the identification of the component itself. [Item 1-3-4-19] A system for culturing cells, comprising: 1) a cell culture section for containing cells intended for culture; 2) a medium supply section for providing a culture medium or raw materials according to any of the above items; and 3) a cell culture condition adjustment section for adjusting culture conditions as necessary. [Item 1-3-4-20] A culture medium suitable for transporting cells containing the culture medium components described in any of the above items.[Item 1-3-4-21] A method for managing cell culture for cells suitable for cultivation using the culture medium described in any one of the above items, comprising: 1) providing information about the culture medium with respect to cells provided by the user; 2) assigning identifiable labels to the cells and the culture medium; and 3) managing whether the cells and the culture medium are compatible by referring to the labels.

[0065] [Item 1-3-4-22] The use of (1), (3) and (4) described in Item 1-3-4-1 for culturing cells (preferably mesenchymal stem cells). [Item 1-3-4-23] The use of Item 1-3-4-22 for culturing cells (preferably mesenchymal stem cells), comprising the features of any one of Items 1-3-4-2 to 1-3-4-6C. [Item 1-3-4-24] A method for maintaining or improving the quality of cells (preferably mesenchymal stem cells) (preferably, the expression level of at least one selected from the group consisting of CD44, CD73, CD90 and CD105, and / or differentiation potential after long-term passage), comprising the step of contacting the cells in a culture medium containing the components described in Item 1-3-4-1. [Item 1-3-4-25] The method according to Item 1-3-4-24, comprising the features of any one of Items 1-3-4-2 to 1-3-4-6C. [Item 1-3-4-26] Cells or cell populations described...

Claims

(1) Hallucinonides (Here, R １ and R ２ Each is independently selected from the group consisting of hydrogen, alkyl, alkoxy, hydroxy, and OR (where R is hydrogen or alkyl), or R １ and R ２ They come together to form a ring, R ３ is a halo or hydrogen, and R ４ (where is a halo or a substituted hydroxyl) or a pharmaceutically acceptable salt or solvate thereof (2) ALK4 / 7 inhibitor or TGFβ receptor type 1 kinase inhibitor (3) BRD7 / 9 dual degradation factor or BRD7 / 9 binding molecule (degradation factor) (4) LATS1 / 2 inhibitor, PKA inhibitor, or AKT1 inhibitor (5) CK1 / 2 inhibitor, insulin receptor Tyr kinase inhibitor, PKA inhibitor or PKC inhibitor or adenosine kinase inhibitor (6) E-selectin inhibitor, VCAM1 inhibitor or ICAM1 inhibitor; and (7) LATS1 inhibitor + LATS2 inhibitor A culture medium containing two or more components selected from the group consisting of the following. (1')R １ and R ２ Each is independently selected from the group consisting of hydrogen, alkyl, alkoxy, hydroxy, and OR (where R is hydrogen or alkyl). (2') TGFβ receptor type 1 kinase inhibitor (3') BRD7 / 9 binding molecule (degradation factor) (4') AKT1 inhibitor (5') Adenosine kinase inhibitor (6') ICAM1 inhibitor; and (7') LATS1 Inhibitor The culture medium according to claim 1, comprising two or more components selected from the group consisting of the following. The above (1) to (7) are each independently as follows: (1")R １ and R ２ combine to form a ring (2") ALK4 / 7 inhibitor (3”) BRD7 / 9 double decomposition factor (4) LATS1 / 2 inhibitor or PKA inhibitor (5") CK1 / 2 inhibitor, insulin receptor Tyr kinase inhibitor, PKA inhibitor, or PKC inhibitor; (6) E-selectin inhibitor or VCAM1 inhibitor, and (7") LATS2 inhibitor A culture medium containing two or more components selected from the group consisting of the following. (1A) or or a pharmaceutically acceptable salt or solvate thereof; (2A) TGFβ receptor type 1 kinase inhibitor, which is also an ALK4 / 7 inhibitor. (3A) BRD7 / 9 binding molecule (degradation factor) which is also a BRD7 / 9 dual degradation factor. (4A) AKT1 inhibitor, which is also a LATS1 / 2 inhibitor or a PKA inhibitor. (5A) Adenosine kinase inhibitors that are also CK1 / 2 inhibitors, insulin receptor Tyr kinase inhibitors, PKA inhibitors or PKC inhibitors; (6A) ICAM1 inhibitors that are also E-selectin inhibitors or VCAM1 inhibitors; and (7A) LATS1 / 2 Inhibitor The culture medium according to claim 1, comprising two or more components selected from the group consisting of the following. The above (1) to (7) are, respectively (1B) Hallucinonide or a pharmaceutically acceptable salt or solvate (2B) AZ12601011 or a pharmaceutically acceptable salt or solvate thereof (3B) VZ185 or a pharmaceutically acceptable salt or solvate thereof (4B) A-674563 or a pharmaceutically acceptable salt or solvate thereof (5B) 5-iodotubercidin or a pharmaceutically acceptable salt or solvate thereof (6B) ICAM-1-IN-1 or a pharmaceutically acceptable salt or solvate thereof; and (7B) LATS-IN-1 or a pharmaceutically acceptable salt or solvate thereof The culture medium according to claim 1. 　 The culture medium according to any one of claims 1 to 5, wherein the culture medium is for culturing mesenchymal stem cells. 　 A method for producing cells, comprising the step of culturing cells using a culture medium according to any one of claims 1 to 6. 　 The method according to claim 7, wherein the culturing step comprises culturing under conditions that satisfy at least one selected from the group consisting of a specific range of cell numbers, a specific container, and a specific temperature. 　 A cell culture apparatus comprising a culture medium according to any one of claims 1 to 6 or means for providing the culture medium, a container capable of containing the culture medium, and means for providing conditions for culturing cells as necessary. 　 A method for preserving cells, comprising the step of preserving the cells using a culture medium according to any one of claims 1 to 6. 　 A composition for preserving cells, comprising the components described in any one of claims 1 to 6. 　 A cell-containing composition comprising the components and cells described in any one of claims 1 to 6. 　 Cells cultured using the component described in any one of claims 1 to 6. 　 The cell according to claim 13, wherein the properties of the cell are improved. 　 A cell population comprising the cells described in claim 13, wherein the expression of at least one selected from the group consisting of CD44, CD73, CD90, and CD105 is not significantly reduced even after long-term passage. 　 A cell population comprising 50% or more of the cells described in claim 13 or 14, or a cell population described in claim 15, wherein the said cells comprise 50% or more. 　 A pharmaceutical product comprising the cells described in claim 13 or 14, or the cell population described in claim 15 or 16. 　 A method for producing differentiated cells, comprising the step of culturing cells obtained using the culture medium described in any one of claims 1 to 6 under conditions that induce differentiation. 　 Differentiated cells produced by the method described in claim 18. A method for treating or preventing a subject, comprising the step of administering an effective amount of the cells described in claim 13, 14, or 19, the cell population described in claim 15 or 16, or the pharmacopoeia described in claim 17 to a subject in need thereof. A method for quality control of stem cells, comprising determining whether the culture medium described in any one of claims 1 to 6 has a coefficient C that correlates with it. 　 A system for culturing cells, 1) A cell culture section for containing cells intended for culture, 2) A culture medium providing unit that provides the culture medium or raw materials according to any one of claims 1 to 6, 3) A cell culture condition adjustment unit that adjusts the culture conditions as needed. A system that includes this. 　 A culture medium suitable for transporting cells, comprising the culture medium components described in any one of claims 1 to 6. 　 A method for managing cell culture for cells suitable for cultivation using the culture medium described in any one of claims 1 to 6, 1) A step of providing information regarding the culture medium for the cells provided by the user, 2) A step of assigning an identifiable label to the cells and the culture medium, 3) A step of checking whether the cells and the culture medium are compatible by referring to the label. A method of including.

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