A method for preparing and applying a ceramide derived from the golden yellow mushroom (Pleurotus citrinipileatus), possessing moisturizing, brightening, anti-aging and skin barrier-maintaining properties.

Abstract

The present invention relates to the field of active ingredient preparation, and more particularly to a method for preparing and applying a ceramide derived from the golden oyster mushroom (Pleurotus citrinipileatus), possessing moisturizing, brightening, anti-aging, and skin barrier-maintaining properties. The method comprises the following steps: collection and pretreatment; fermentation treatment; separation and extraction; and refining. The advantage lies in the fact that the method for preparing and applying this ceramide, through the optimization of fermentation conditions and separation and extraction techniques, allows for the efficient preparation of the ceramide from the golden oyster mushroom (Pleurotus citrinipileatus) and increases the yield and purity of the ceramide.

Description

2. Environmental pollution associated with existing ceramide production methods. This method improves the yield and purity of ceramides, while reducing production costs and environmental impact. To achieve this objective, the invention proposes the following technical solution: a method for preparing a ceramide from the golden yellow mushroom (Pleurotus citrinipileatus), possessing moisturizing, brightening, anti-aging and skin barrier maintenance properties, comprising the following steps: collection and pretreatment; fermentation; separation and extraction; purification. Preferably, the specific collection and pretreatment operations include: Fresh fruiting bodies of the golden-yellow mushroom (Pleurotus citrinipileatus) are collected, selecting samples of golden-yellow color and firm texture; each kilogram of fruiting bodies of this mushroom contains approximately 200 mg of phytosphingosine.;15 washing to remove surface impurities, then cutting into small pieces of 0.5 cm×0.5 cm to facilitate subsequent processing. Preferably, the specific fermentation operations include: introducing the pieces of the golden-yellow mushroom (Pleurotus citrinipileatus) into a fermenter, followed by the addition of an appropriate quantity of water and a special fermentation agent, 20 maintaining a ratio of 10:1 between the fermentation liquid and the fruiting bodies of the golden-yellow mushroom (Pleurotus citrinipileatus); fermentation at a constant temperature of 30°C for 24 hours, with the conversion of ergotamine reaching approximately 50% after this time.Preferably, the specific separation and extraction operations include: 25 after the end of fermentation, the fermentation liquid and the sphingosines are separated by filtration, obtaining approximately 10 liters of fermented liquid; the fermentation liquid is then concentrated to one-fifth of its initial volume to remove excess water; analysis by high-performance liquid chromatography (HPLC) shows that the ceramide concentration in the concentrate can reach 2 mg per milliliter. Preferably, the specific purification operations include: the ceramide obtained by extraction is purified by crystallization, filtration, and drying to remove unreacted sphingosine and other impurities; The purity of the purified ceramide can reach more than 1 to 3%.5 Application of the ceramide obtained according to the method of preparation of a ceramide from the golden yellow mushroom (Pleurotus citrinipileatus), possessing moisturizing, brightening, anti-aging and skin barrier maintenance properties, in the field of hydration.Application of ergothioneine obtained according to the method of preparing a ceramide from the golden-yellow mushroom (Pleurotus citrinipileatus), possessing moisturizing, brightening, anti-aging and skin barrier properties, in the field of skin brightening. Application of ergothioneine obtained according to the method of preparing a ceramide from the golden-yellow mushroom (Pleurotus citrinipileatus), possessing moisturizing, brightening, anti-aging and skin barrier properties, in the field of combating aging. Application of ergothioneine obtained according to the method of preparation of a ceramide from the golden yellow mushroom (Pleurotus citrinipileatus), possessing moisturizing, brightening, anti-aging and skin barrier maintenance properties, in the field of maintaining the skin barrier.20 Compared to existing techniques, the beneficial effects of the present invention are as follows: The method for preparing and applying a ceramide derived from the golden oyster mushroom (Pleurotus citrinipileatus), having moisturizing, whitening, anti-aging, and skin barrier properties, proposed in the present invention, allows, through the optimization of the fermentation conditions and the separation and extraction techniques, the efficient production of ceramide from the golden oyster mushroom (Pleurotus citrinipileatus). This technology improves the yield and purity of the ceramide, with the yield reaching 10% and the yield 1 to 3% or more. The biological fermentation method replaces traditional chemical synthesis, thus avoiding the environmental pollution problems that may arise. 30 BE2025 / 5756 4 during chemical processes. In addition, the raw material used, the golden yellow mushroom (Pleurotus citrinipileatus), is a renewable resource, favorable to environmental protection and sustainable development.The ceramide thus prepared presents vast application prospects in the pharmaceutical, cosmetic, and health product fields. In the pharmaceutical field, it can be used to maintain the functioning of the cardiovascular system and to treat associated diseases; in the cosmetic field, it constitutes a key ingredient in moisturizing, whitening, and photoprotective products; in the field of food supplements, it can be used as an additive in functional foods or beverages to strengthen immunity, protect the skin, and delay aging. This invention therefore makes it possible to solve the problems of high cost and significant pollution associated with existing preparation methods, while improving the yield and purity of ceramide, and reducing production costs and environmental impact. Description of drawings: Figure 1 is a schematic diagram of the process according to the present invention.Specific embodiments 15 In order to describe clearly and completely the object and process of the present invention, and to make its advantages more understandable, examples of embodiments of the invention are explained in detail below with reference to the figures. It should be understood that the specific examples described herein constitute only a part of the examples of embodiments of the invention and not all of them, serving only to illustrate the examples 20 of embodiments of the invention without limiting their scope. All other embodiments achieved by a person skilled in the art without additional creative effort fall within the scope of protection of the present invention. Example of embodiment 1 Please refer to i.