A biological pesticide composition for preventing and treating thrips palmi

Abstract

The present application belongs to the technical field of biological pesticides, and particularly relates to a biological pesticide composition for preventing and treating cowpea leafhoppers. The effective component of the biological pesticide is compounded by Java Cordyceps bacteria and abamectin or spinetoram; wherein the Java Cordyceps bacteria is 500 billion spores / gram of Java Cordyceps bacteria JS001. When the Java Cordyceps bacteria is compounded with abamectin or spinetoram in the biological pesticide composition, the cowpea leafhoppers show a synergistic effect, the prevention and treatment effect can be improved, the pesticide usage amount can be reduced, and the prevention and treatment cost can be reduced.

Description

Technical Field

[0001] This invention belongs to the field of mango thrips control technology, specifically relating to a biological pesticide composition for controlling cowpea thrips. Background Technology

[0002] Cowpea thrips, belonging to the family Thripidae in the order Thysanoptera, are among the major pests of cowpeas. They include species such as the bean thrips (Megalurothrips usitatus Bagnall) and the flower thrips (Frankliniella intonsa Trybom). Cowpea thrips suck the sap from the flowers and pods, causing blackening of the pod heads and tails, blackening and drop of flower buds, and they can also transmit viral diseases, reducing cowpea yield and quality.

[0003] Pesticides are the primary means of controlling cowpea thrips. Currently registered insecticides for this purpose include abamectin, spinosad, thiamethoxam, acetamiprid, cyantraniliprole, and Metarhizium anisopliae. However, due to frequent and improper use of insecticides, pest resistance has accumulated, reducing the effectiveness of pest control.

[0004] Cordyceps militaris JS001 is an insecticide derived from a pathogenic fungus discovered by the Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences. Cordyceps militaris belongs to the family Cordycepsaceae and the genus Cordyceps, and is a highly related insecticidal fungus to Cordyceps sinensis. JS001 has a unique mechanism of action and a broad insecticidal spectrum, effectively controlling piercing-sucking pests such as whiteflies, aphids, and rice planthoppers, as well as chewing pests such as beet armyworms and mites. The Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences, has registered 50 billion spores / g of Cordyceps militaris JS001 technical material and 5 billion spores / mL of Cordyceps militaris JS001 dispersible oil suspension.

[0005] The scientific and rational combination of pesticides is one of the important means of pest resistance management, which has the advantages of slowing down the development of pesticide resistance, improving efficacy, and reducing control costs. Currently, there are no reports of combining *Cordyceps militaris* with abamectin or spinosad.

[0006] The information disclosed in this background section is intended only to enhance the understanding of the overall background of the invention and should not be construed as an admission or in any way implying that the information constitutes prior art known to those skilled in the art. Summary of the Invention

[0007] The purpose of this invention is to provide a biological pesticide composition for controlling cowpea thrips, in order to solve the problem that the frequent and improper use of insecticides leads to the continuous accumulation of pesticide resistance in pests, resulting in a decrease in the control effect of pests.

[0008] To achieve the above objectives, the present invention provides the following technical solution:

[0009] A biological pesticide composition, wherein the active ingredient of the biological pesticide is a compound of Cordyceps militaris and abamectin or spinosad; wherein the Cordyceps militaris is Cordyceps militaris JS001 with 50 billion spores / gram.

[0010] Preferably, the mass ratio of the *Cordyceps militaris* to avermectin is 1-35:7-1.

[0011] Preferably, the mass ratio of the *Cordyceps militaris* to ethyl spinosad is 1-10:2-1.

[0012] The present invention also provides a biopesticide formulation comprising the aforementioned biopesticide composition.

[0013] The present invention also provides the use of the described biopesticide composition or the described biopesticide formulation in the control of cowpea thrips.

[0014] Preferably, the cowpea thrips include bean thrips and flower thrips.

[0015] Compared with the prior art, the present invention has the following beneficial effects:

[0016] (1) When the biological pesticide composition of the present invention is combined with abamectin or spinosad, it exhibits a synergistic effect on cowpea thrips, which can improve the control effect, reduce the amount of pesticide used, and reduce the control cost.

[0017] (2) The insecticidal mechanism of Cordyceps militaris in the biological pesticide composition of the present invention is different from that of abamectin or spinosad, which can slow down the development of pesticide resistance in pests and extend the service life of the pesticide.

[0018] (3) The biopesticide composition of the present invention is environmentally friendly and green. Detailed Implementation

[0019] The technical solution of this invention patent will be clearly and completely described below. Obviously, the described embodiments are only some, not all, of the embodiments of this invention. Based on the embodiments of this invention, all other embodiments obtained by those skilled in the art without inventive effort are within the scope of protection of this invention.

[0020] Example Indoor bioactivity test

[0021] 1. Test reagents

[0022] JS001 technical material of Cordyceps militaris with 50 billion spores / gram (Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences), 92% abamectin technical material (Shandong Qifa Pharmaceutical Co., Ltd.), and 81.2% spinosad technical material (Corteva Agricultural Technology Co., Ltd.).

[0023] The technical grade of Cordyceps militaris JS001 was diluted with 0.1% Tween-80 aqueous solution to form a suspension as a single-agent stock solution; the technical grade of abamectin and spinosad were then diluted with 0.1% Tween-80 aqueous solution to form a single-agent stock solution; multiple formulations were prepared, and each single agent and each formulation mixture was set with 5 gradient mass concentrations according to the proportional method, with 50 mL of drug solution for each mass concentration, for later use.

[0024] 2. Test pests

[0025] Flower thrips were collected from cowpea fields and raised for five generations in an artificial climate chamber in the laboratory. Then, healthy and active second-instar nymphs of uniform size were selected as test subjects.

[0026] 3. Test methods (refer to NY / T 1154.6-2006 Guidelines for Indoor Bioassay of Pesticides - Part 6: Insecticidal Activity Test - Immersion Method).

[0027] The test pests were immersed in the pesticide solution for 10 seconds. Excess pesticide was absorbed with filter paper, and the pests were then transferred back to their original rearing conditions. Each treatment was repeated four times, with 25 pests immersed in each replicate. A treatment containing only 0.1% Tween-80 was included as a blank control. After 72 hours, the mortality rate of the test pests was assessed. The total number of pests and the number of dead pests were recorded for each treatment, and the corrected mortality rate was calculated. Regression analysis was performed using DPS software on the logarithmic values ​​of pesticide concentrations and the corrected mortality rate probabilities for each treatment to calculate the LC50 of the pesticide for each treatment. 50 .

[0028]

[0029] In the above formula: P -- mortality rate, in %; K -- number of dead insects; N -- total number of insects treated.

[0030]

[0031] In the above formula: P1 -- corrected mortality rate, in %; P t --Treatment mortality rate, in %; P0--Control mortality rate, in %.

[0032] 5. Pharmaceutical Evaluation

[0033] The co-toxicity coefficient (CTC value) of the mixture was calculated according to Sun Yunpei's method, and the combined effect was classified according to the following criteria: a co-toxicity coefficient (CTC) ≥ 120 indicates a synergistic effect; a co-toxicity coefficient (CTC) ≤ 80 indicates an antagonistic effect; and 80 < co-toxicity coefficient (CTC) < 120 indicates an additive effect.

[0034] The experimental results are shown in Table 1-3.

[0035] Table 1. Indoor bioactivity assay of Cordyceps militaris JS001 and avermectin in combination with thrips.

[0036] Drug Name and Proportion <![CDATA[LC 50 (mg / L)]]> ATI TTI CTC Java Cordyceps JS001 61.5714 100.0000 -- -- Avermectin 12.9306 476.1682 -- -- Java Cordyceps JS001: Avermectin (1:7) 8.7304 705.2529 429.1471 164.3383 Java Cordyceps JS001: Avermectin (1:3) 4.8371 1272.8991 382.1261 333.1097 Java Cordyceps JS001: Avermectin (1:1) 11.5847 531.4889 288.0841 184.4909 Java Cordyceps JS001: Avermectin (3:1) 21.5038 286.3280 194.0420 147.5598 Java Cordyceps JS001: Avermectin (7:1) 23.9260 257.3410 147.0210 175.0369 Java Cordyceps militaris JS001: Avermectin (15:1) 31.1769 197.4904 123.5105 159.8977 Java Cordyceps militaris JS001: Avermectin (25:1) 44.4313 138.5766 114.4680 121.0615 Java Cordyceps militaris JS001: Avermectin (35:1) 36.8206 167.2200 110.4491 151.4000

[0037] As shown in Table 1, within a mass ratio of 1-35:7-1, the co-toxicity coefficient of the combination of Cordyceps militaris JS001 and abamectin is greater than 120, indicating that the combination of Cordyceps militaris JS001 and abamectin has a synergistic effect on the biological activity of thrips.

[0038] Table 2. Indoor bioactivity assay of Cordyceps militaris JS001 and ethyl spinosad in combination with thrips.

[0039]

[0040]

[0041] As shown in Table 2, within a mass ratio of 1-10:2-1, the co-toxicity coefficient of the combination of Cordyceps militaris JS001 and ethyl spinosad is greater than 120, indicating that the combination of Cordyceps militaris JS001 and ethyl spinosad has a synergistic effect on the biological activity of flower thrips.

[0042] In conclusion, when Cordyceps militaris is combined with abamectin or spinosad, it exhibits a synergistic effect against cowpea thrips, which can improve control efficacy, reduce pesticide usage, and lower control costs.

[0043] The foregoing description of specific exemplary embodiments of the invention is for illustrative and explanatory purposes. These descriptions are not intended to limit the invention to the precise forms disclosed, and it will be apparent that many changes and variations can be made in accordance with the foregoing teachings. The exemplary embodiments were chosen and described in order to explain the specific principles of the invention and its practical application, thereby enabling those skilled in the art to implement and utilize various different exemplary embodiments of the invention, as well as various different choices and variations. The scope of the invention is intended to be defined by the claims and their equivalents.

Claims

1. A biological pesticide composition, characterized in that, The active ingredient of the biopesticide is a compound of Cordyceps militaris and abamectin or spinosad; wherein the Cordyceps militaris is Cordyceps militaris JS001 with 50 billion spores / gram; The mass ratio of Cordyceps militaris to abamectin is 1-35:7-1; The mass ratio of Cordyceps militaris to ethyl spinosad is 1-10:2-1.

2. A biological pesticide formulation, characterized in that, Includes the biological pesticide composition according to claim 1.

3. The use of the biological pesticide composition of claim 1 or the biological pesticide formulation of claim 2 in the control of cowpea thrips, characterized in that, The cowpea thrips mentioned are flower thrips.

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