A medicine and fertilizer for preventing and treating papaya tumor disease

Abstract

This invention belongs to the field of pesticide and fertilizer technology, specifically a pesticide and fertilizer for controlling papaya gall disease. It includes a synergistic bactericidal composition, the active ingredient of which is a combination of glycyrrhizin and fluoxastrobin. This pesticide and fertilizer for controlling papaya gall disease contains both bactericidal and boron fertilizer. The bactericidal component enhances the control effect against papaya anthracnose, while the boron fertilizer supplements the boron element needed for papaya growth, thus effectively preventing papaya gall disease. This single application can control both anthracnose and papaya gall disease, promoting the normal and healthy growth of papaya.

Description

Technical Field

[0001] This invention belongs to the field of pesticide and fertilizer technology, specifically to a pesticide and fertilizer for the prevention and treatment of papaya gall disease. Background Technology

[0002] Papaya gall disease is a physiological disorder primarily caused by boron deficiency. Symptoms include smaller leaves, shortened petioles, browning and death of young leaf tips, leaf curling, and fruit drop in large quantities even at a very young age. The fruit exhibits latex exudation from the young fruit stage to early maturity. After the sap flows out, the fruit peel gradually rots and softens, turning brown. Unrotted areas develop uneven, wart-like protrusions. Severely affected fruit results in seed degeneration and abortion. Applying boron fertilizer can effectively control papaya gall disease in agricultural production.

[0003] In addition, anthracnose is a common disease in papaya production, caused by fungi such as *Colletotrichum gloeosporioides* and *Colletotrichum capsici*. Anthracnose primarily affects the fruit, leaves, and petioles, impacting both yield and quality. In agricultural production, chemical pesticides are commonly used to control anthracnose, but long-term application of single-component chemical pesticides can easily lead to pesticide resistance. As pathogen resistance develops, the effectiveness of chemical pesticides gradually decreases. While increasing pesticide dosage can improve control to some extent, it also increases pesticide residues and environmental pressure. Therefore, providing a novel chemical pesticide is essential.

[0004] Glycyrrhizin belongs to the isoflavone class of compounds and is mainly isolated from licorice root. Its CAS number is 59870-68-7, and its molecular formula is C2. 20 H 20 O4, the structural formula is as follows:

[0005]

[0006] Currently, the main applications of glycyrrhizin are concentrated in the medical field, demonstrating significant value in anti-oxidation, anti-inflammation, anti-atherosclerosis, lipid-lowering, blood pressure-lowering, and anti-obesity effects. Existing technologies have also reported the activity of glycyrrhizin against agricultural fungi and bacteria. For example, CN201510475579.1 discloses the use of an isoflavone compound in the preparation of agricultural fungicides. This patent discloses the structural formula of glycyrrhizin and its inhibitory effects on fungi such as Fusarium head blight of wheat, anthracnose of tea, gray mold of strawberry, late blight of potato, blast fungus of rice, soft rot of Chinese cabbage, bacterial wilt of tomato, wilt of ginger, and canker of kiwifruit.

[0007] Combining different pesticide components is a convenient and effective way to develop new chemical agents. The inventors discovered through experiments that combining glycyrrhizin with fluoxastrobin, bromodiphenyl ether, or thifluzamide has a synergistic effect against papaya anthracnose and can be used to develop new chemical agents. However, these chemical agents are only effective against fungal diseases such as papaya anthracnose and have almost no effect on physiological diseases of papaya. If boron, used to control papaya gall disease, is added to this chemical agent to form a medicated fertilizer, then a single fertilization could control both papaya anthracnose and papaya gall disease.

[0008] The information disclosed in this background section is intended only to enhance the understanding of the overall background of the invention and should not be construed as an admission or in any way implying that the information constitutes prior art known to those skilled in the art. Summary of the Invention

[0009] The purpose of this invention is to provide a medicated fertilizer for preventing papaya gall disease, which contains both bactericidal and boron fertilizer. The bactericidal components can improve the control effect against papaya anthracnose, while the boron fertilizer can supplement the boron element required for papaya growth, thereby effectively preventing papaya gall disease.

[0010] To achieve the above objectives, the present invention provides the following technical solution:

[0011] A synergistic bactericidal composition, wherein the active ingredient of the synergistic bactericidal composition is composed of glycyrrhizin combined with fluoxastrobin, bromodiphenyl ether, or thifluzamide.

[0012] Preferably, the mass ratio of glycyrrhizin to fluoxetine is 1-5:15-1.

[0013] Preferably, the mass ratio of glycyrrhizin to bromodiphenyl ether is 1-20:3-1.

[0014] Preferably, the mass ratio of glycyrrhizin to thifluzamide is 1-10:60-1.

[0015] This invention also provides a medicated fertilizer for controlling papaya gall disease, comprising the following components by weight percentage: 0.5-8% of the synergistic bactericidal composition, 25-35% of water-soluble boron fertilizer, 6-8% of dispersant, 2-4% of wetting agent, and filler to 100%. The preparation method is as follows: the synergistic bactericidal composition, water-soluble boron fertilizer, dispersant, wetting agent, and filler are mixed evenly, and then pulverized using an air jet mill and mixed thoroughly. The application method is foliar spraying.

[0016] Preferably, the water-soluble boron fertilizer is sodium octaborate tetrahydrate.

[0017] The present invention also provides the application of the aforementioned synergistic bactericidal composition in the prevention and control of papaya anthracnose.

[0018] The present invention also provides the application of the aforementioned medicated fertilizer in the prevention and control of papaya gall and anthracnose.

[0019] Compared with the prior art, the present invention has the following beneficial effects:

[0020] (1) The fertilizer and pesticide of this invention for preventing papaya gall disease contains both bactericidal and boron fertilizers. The bactericidal components can improve the control effect against papaya anthracnose, while the boron fertilizer can supplement the boron element needed for papaya growth, thus effectively preventing papaya gall disease. In this way, a single application can control both papaya anthracnose and papaya gall disease, which is beneficial to the normal and healthy growth of papaya.

[0021] (2) The synergistic bactericidal composition of the present invention is composed of glycyrrhizin and fluoxastrobin, bromodiphenyl ether or thifluzamide. It has a synergistic effect of greater than 1.5 against anthracnose of papaya, which can improve the control effect of anthracnose of papaya, help reduce the dosage of pesticides and reduce environmental pollution. Detailed Implementation

[0022] The present invention can be better understood from the following embodiments. However, those skilled in the art will readily understand that the descriptions in the embodiments are for illustrative purposes only and should not, and will not, limit the invention as detailed in the claims.

[0023] Example 1 Screening of control agents

[0024] 1. Test strain: Colletotrichum gloeosporioides Penz., isolated from infected papaya leaves and preserved on PDA medium.

[0025] 2. Test reagents

[0026] 98% glycyrrhizin technical grade (Shanghai Yuanye Biotechnology Co., Ltd.)

[0027] 96% Fluoxastrobin Technical Grade (Syngenta Nantong Crop Protection Co., Ltd.)

[0028] 95% bromochlorothalonil technical grade (Jiangsu Tuoqiu Agrochemical Co., Ltd.)

[0029] 97% Thifluzamide Technical Grade (Jiangsu Haoshoucheng Wayne Agrochemical Co., Ltd.)

[0030] The test reagent was first dissolved in dimethyl sulfoxide, then diluted with 0.1% Tween-80 aqueous solution to prepare a single-agent stock solution. Multiple formulations were set up, and five mass concentration gradients were set for each single agent and formulation mixture according to the proportional method.

[0031] 3. Test Methods

[0032] The mycelial growth rate method was used. 9 mL of pre-melted PDA medium was added to a sterile Erlenmeyer flask. Quantitatively measured 1 mL of drug solution, from low to high concentration, was added to each flask. After thorough mixing, the solution was poured into 9 cm diameter Petri dishes to prepare drug-containing plates of the corresponding concentrations. A blank control without the drug was also included, with five replicates for each treatment. Mycelial cakes were cut from the edge of the test strain colonies using a 5 mm punch and inoculated into the center of both the drug-containing and blank control plates. The plates were then capped and incubated at 25°C. When the diameter of the blank control colonies reached two-thirds of the Petri dish diameter, the colony diameter was measured using the cross-sectional method, and the inhibition rate of mycelial growth by different treatments was calculated.

[0033]

[0034] 4. Data Analysis: Statistical analysis was performed using DPS software. Linear regression was conducted with the logarithm of the fungicide concentration as x and the corresponding mycelial growth inhibition rate probability value as y to derive the virulence regression equation and the EC50 of the fungicide against the target pathogen. 50 The synergistic effect was evaluated using the Wadlley method.

[0035] 5. Measurement Results

[0036] The synergistic effect of the drug was evaluated based on the calculated synergistic coefficient (SR). SR ≥ 1.5 indicates a synergistic effect; SR ≤ 0.5 indicates an antagonistic effect; and 0.5 < SR < 1.5 indicates an additive effect. The results are shown in Table 1-3.

[0037] Table 1. Virulence of glycyrrhizin and fluoxetine combined with *Colletotrichum candida*

[0038]

[0039] As shown in Table 1, the synergistic effect coefficients of glycyrrhizin and fluoxetine against Colloidal anthracnose within a mass ratio of 1-5:15-1 ranged from 1.5800 to 10.7763, all greater than 1.5, indicating a synergistic effect.

[0040] Table 2. Virulence of glycyrrhizin and bromodiphenyl ether combined with *Colletotrichum candida*.

[0041]

[0042] As shown in Table 2, the synergistic effect coefficients of glycyrrhizin and bromuconazole against Colloidal anthracnose within a mass ratio of 1-20:3-1 ranged from 1.5362 to 13.1979, all greater than 1.5, indicating a synergistic effect.

[0043] Table 3. Virulence of glycyrrhizin and thifluzamide combination against Colletotrichum candida.

[0044]

[0045] As shown in Table 3, the synergistic effect coefficients of glycyrrhizin and thifluzamide against Colloidal anthracnose in mass ratios of 1:60-5 or 5-10:1 ranged from 1.5335 to 6.8799, all greater than 1.5, indicating a synergistic effect.

[0046] In summary, when glycyrrhizin is combined with fluoxastrobin, bromodiphenyl ether, or thifluzamide, it exhibits a strong synergistic effect against Colletotrichum gloeosporioides, which can improve the control of papaya anthracnose, reduce pesticide dosage, alleviate environmental pressure, and lower pesticide residues.

[0047] Example 2

[0048] A fertilizer for controlling papaya gall disease comprises the following components by weight percentage: 3% synergistic bactericidal composition, 25% sodium octaborate tetrahydrate, 8% sodium lignosulfonate, 2% bleaching powder BX, and 100% silica; wherein the synergistic bactericidal composition is a mixture of glycyrrhizin and fluoxastrobin in a weight ratio of 1:5.

[0049] Example 3

[0050] A fertilizer for controlling papaya gall disease comprises the following components by weight percentage: 2% synergistic bactericidal composition, 30% sodium octaborate tetrahydrate, 6% sodium dodecylbenzenesulfonate, 4% pyroxene BX, and attapulgite soil to make up to 100%; wherein the synergistic bactericidal composition is a mixture of glycyrrhizin and bromodiphenyl ether in a weight ratio of 20:1.

[0051] Example 4

[0052] A fertilizer for preventing papaya gall disease comprises the following components by weight percentage: 6% synergistic bactericidal composition, 32% sodium octaborate tetrahydrate, 8% sodium dodecylbenzenesulfonate, 3% soapberry powder, and bentonite to make up to 100%; wherein the synergistic bactericidal composition is a mixture of glycyrrhizin and thifluzamide in a weight ratio of 5:1.

[0053] The foregoing description of specific exemplary embodiments of the invention is for illustrative and explanatory purposes. These descriptions are not intended to limit the invention to the precise forms disclosed, and it will be apparent that many changes and variations can be made in accordance with the foregoing teachings. The exemplary embodiments were chosen and described in order to explain the specific principles of the invention and its practical application, thereby enabling those skilled in the art to implement and utilize various different exemplary embodiments of the invention, as well as various different choices and variations. The scope of the invention is intended to be defined by the claims and their equivalents.

Claims

1. A synergistic fungicidal composition, characterized by, The active ingredient of the synergistic fungicidal composition is composed of glycyrrhizin and fluazinam, and the mass ratio of the glycyrrhizin to the fluazinam is 1-5:15-1.

2. A pharmaceutical fertilizer for controlling papaya bunchy top disease, characterized by, The following components are included in percentage by mass: The synergistic fungicidal composition of claim 1 0.5-8%, water-soluble boron fertilizer 25-35%, dispersing agent 6-8%, wetting agent 2-4%, and filler supplementing to 100%.

3. The fertilizer of claim 2, wherein The water-soluble boron fertilizer is sodium octaborate tetrahydrate.

4. The use of the synergistic fungicidal composition of claim 1 in the prevention and treatment of anthracnose of papaya.

5. The use of the pharmaceutical fertilizer of claim 2 in the prevention and treatment of gummy stem and anthracnose of papaya.

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