A molecular identity card of the genus Aristolochia, its combination and its application

By designing molecular identification cards for the Aristolochia genus and their combinations, and combining PCR amplification and high-throughput sequencing technologies, the problem of accuracy in identifying Aristolochia plant components was solved, and efficient identification of Aristolochia components in complex samples was achieved.

CN117248050BActive Publication Date: 2026-06-30INST OF MEDICINAL PLANT DEV CHINESE ACADEMY OF MEDICAL SCI
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Patent Information

Application Number
CN202311079772.4
Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Filing Date
2023-08-25
Publication Date
2026-06-30
Estimated Expiration
2043-08-25

AI Technical Summary

Technical Problem

Existing technologies struggle to accurately identify components of Aristolochia species, especially in complex samples where differentiation and identification are difficult. Furthermore, the application of conventional DNA barcoding is limited in samples with severely degraded DNA.

Method used

A molecular identity card and its combination for the genus Aristolochia were designed. Based on the matK gene sequence, nucleotide sequences were screened using bioinformatics techniques and BLAST was performed in the NCBI database to obtain specific nucleotide sequences. Combined with PCR amplification and high-throughput sequencing technology, Aristolochia components in the test samples were detected.

Benefits of technology

It enables accurate identification of Aristolochia components in plant materials and deep-processed samples, has stronger applicability and a wider range of applications, and can identify samples with severely degraded DNA.

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Abstract

This invention relates to the field of molecular biology, specifically disclosing a molecular identification code for the genus *Aristolochia*, its combination, and its applications. The molecular identification code for *Aristolochia* of this invention is the nucleotide sequence shown in SEQ ID No. 1. The molecular identification code combination for *Aristolochia* is the nucleotide sequence shown in SEQ ID No. 1-6. This invention can determine the presence of *Aristolochia* species in a test sample by detecting whether the PCR amplification product or raw high-throughput sequencing data contains the nucleotide sequence shown in SEQ ID No. 1 or SEQ ID No. 1-6. This invention can accurately identify *Aristolochia* species, has broader applicability, and can detect the presence of *Aristolochia* species in all samples from which DNA can be extracted. It enables rapid and accurate identification of original plants, medicinal materials, powders, traditional Chinese medicine preparations, and mixed food materials of the *Aristolochia* genus.
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