A synergistic glufosinate-ammonium aqueous solution and a preparation method thereof

Abstract

The application discloses a synergistic glufosinate-ammonium aqueous agent and a preparation method thereof, and belongs to the field of pesticide preparations. In the glufosinate-ammonium, phycocyanin is added to promote absorption of light energy and transmission of light energy, adenosine triphosphate is added to promote leaf respiration, and alkyl polyglycoside and cocamide propyl betaine are added to have wetting and spreading effects. After application, photosynthesis and respiration of plants can be significantly improved, the glufosinate-ammonium can penetrate the cuticle and wax layer into weeds, thereby effectively improving the absorption of the glufosinate-ammonium by the plants, accelerating the exertion of the drug efficacy, and improving the low-temperature weeding activity of the glufosinate-ammonium aqueous agent.

Description

Technical Field

[0001] This invention relates to the field of pesticide formulation technology, specifically to a synergistic glufosinate aqueous solution and its preparation method. Background Technology

[0002] Glufosinate is a phosphonic acid herbicide, an inhibitor of glutamine synthesis, and a non-selective contact herbicide with partial systemic action. Shortly after application, ammonium metabolism in the plant is disrupted, leading to the accumulation of cytotoxic ammonium ions within the plant. Simultaneously, photosynthesis is severely inhibited, ultimately causing the entire plant to wither and die.

[0003] Spectrum of herbicides: Glufosinate has a broad spectrum of herbicides, effectively controlling almost all annual and perennial weeds in farmland. It can be used in orchards, vineyards, potato fields, and non-cultivated land to control annual and perennial dicotyledonous and grassy weeds, such as barnyard grass, crabgrass, barnyard grass, wild barley, ryegrass, foxtail grass, golden foxtail grass, wild wheat, wild corn, duckweed, sedge, fescue, etc. It can also control broadleaf weeds such as lambsquarters, amaranth, knotweed, shepherd's purse, black nightshade, chickweed, purslane, cleavers, sow thistle, field thistle, field bindweed, and dandelion, and has some effect on sedges and ferns.

[0004] Glufosinate is a phosphoric acid herbicide, a glutamine synthesis inhibitor, and a non-selective, broad-spectrum, contact herbicide with partial systemic activity. Shortly after application, it disrupts ammonium metabolism in the plant, leading to the accumulation of cytotoxic ammonium ions and severely inhibiting photosynthesis, thus achieving weed control. Glufosinate is characterized by high activity, low toxicity, and good environmental compatibility. It can be transported upwards within the xylem via transpiration and downwards into the phloem, but its translocation is relatively poor. Studies have shown that the herbicidal activity of glufosinate decreases significantly under low-temperature conditions. This is because low temperatures significantly reduce both respiration and photosynthesis of glufosinate, leading to decreased systemic and translocation efficiency.

[0005] Currently, the commonly used synergists for glufosinate-ammonium aqueous solutions on the market are mainly surfactants such as alkyl glycosides, betaine, and sodium lauryl sulfate of fatty alcohol polyoxyethylene ether. The addition of surfactants can improve the wetting and spreading effect of the solution, but it is difficult to fundamentally improve the low-temperature herbicidal activity of glufosinate-ammonium. Summary of the Invention

[0006] To address the shortcomings of existing technologies, the present invention aims to provide a synergistic glufosinate aqueous solution containing phycocyanin (which promotes light energy absorption and transmission), adenosine triphosphate (ATP) (which promotes leaf respiration), alkyl glycosides (which have wetting and spreading effects), and cocamidopropyl betaine (which significantly enhances plant photosynthesis and respiration after application, thereby effectively improving plant absorption of glufosinate, accelerating efficacy, and enhancing low-temperature herbicidal activity, as well as its preparation method.

[0007] To achieve the above objectives, the present invention adopts the following technical solution: a synergistic glufosinate aqueous solution, wherein the synergistic glufosinate aqueous solution comprises the following components by weight percentage: 5-40% glufosinate active ingredient, 0.1-1% phycocyanin, 0.1-1% adenosine triphosphate, 0.1-10% alkyl glycoside, 0.1-10% cocamidopropyl betaine, 0-5% anionic surfactant, and the balance being water.

[0008] Furthermore, the weight ratio of phycocyanin to adenosine triphosphate in the enhanced glufosinate aqueous solution is 1:1.

[0009] Furthermore, a synergistic glufosinate aqueous solution comprises the following components by weight percentage: 8-40% glufosinate active ingredient, 0.3-1% phycocyanin, 0.3-1% adenosine triphosphate, 0.8-10% alkyl glycoside, 0.5-10% cocamidopropyl betaine, 0.7-5% anionic surfactant, and the balance being water.

[0010] Furthermore, a synergistic glufosinate aqueous solution comprises the following components by weight percentage: 10-40% glufosinate active ingredient, 0.5-1% phycocyanin, 0.5-1% adenosine triphosphate, 3-10% alkyl glycoside, 2-10% cocamidopropyl betaine, 2-5% anionic surfactant, and the balance being water.

[0011] Furthermore, the anionic surfactant is sodium fatty alcohol polyoxyethylene ether sulfate, sodium ricinoleate sulfate, or sodium lauroyl sarcosinate.

[0012] Furthermore, the enhanced glufosinate aqueous solution also includes 0.8–1.3 wt% of Fucus vesiculosus extract.

[0013] Furthermore, the enhanced glufosinate aqueous solution also includes 1.2–1.3 wt% of Fucus vesiculosus extract.

[0014] This invention also provides a method for preparing a synergistic glufosinate aqueous solution, comprising the following steps:

[0015] (1) Material taking: Weigh each component of the synergistic glufosinate aqueous solution according to the weight percentage;

[0016] (2) Preparation of the first mixture: Put the water obtained in step (1) into a container, and add glufosinate technical, phycocyanin, adenosine triphosphate, alkyl glycoside, and cocamidopropyl betaine in sequence; stir evenly to obtain the first mixture;

[0017] (3) Mix and stir again: Add the remaining components to the first mixture and continue stirring for 20-30 minutes to obtain the enhanced glufosinate aqueous solution.

[0018] Further, in step (2), the water obtained in step (1) is placed in a container, and glufosinate technical, phycocyanin, adenosine triphosphate, alkyl glycoside, and cocamidopropyl betaine are added in sequence; the mixture is stirred evenly at a temperature of 35-40°C and a stirring speed of 100-150 r / min to obtain the first mixture.

[0019] Further, in step (3), the remaining components are added to the first mixture, and the mixture is stirred for 20 to 30 minutes at a temperature of 35 to 40°C and a stirring speed of 180 to 220 r / min to obtain the enhanced glufosinate aqueous solution.

[0020] This invention discloses an enhanced glufosinate aqueous solution and its preparation method. Addressing the problem that under low temperature and low humidity conditions, the cuticle and waxy layers on the surface of weed leaves are relatively "hard" and "thick," and the stomata are not easily opened, making it difficult for glufosinate to penetrate through the cuticle and waxy layers into the weeds and thus difficult for them to absorb, resulting in poor low-temperature weed control activity of glufosinate aqueous solution. This application adds phycocyanin, which promotes the absorption and transmission of light energy, to glufosinate, along with adenosine triphosphate (ATP), which promotes leaf respiration, and alkyl glycosides and cocamidopropyl betaine, which have wetting and spreading effects. After application, it significantly improves the photosynthesis and respiration of the plant, facilitating the penetration of glufosinate through the cuticle and waxy layers into the weeds, thereby effectively improving the plant's absorption of glufosinate, accelerating the efficacy, and enhancing the low-temperature weed control activity of glufosinate aqueous solution.

[0021] This invention discloses an enhanced glufosinate aqueous solution and its preparation method. Due to the unique microclimate of greenhouses, with differences in temperature and humidity between the greenhouse and the outside environment, and the different microbial balance in the greenhouse soil, glufosinate degradation within the greenhouse is slow, leading to its accumulation in plant roots and stems and increasing the risk of glufosinate phytotoxicity. This application adds an appropriate amount of *Fucus vesiculosus* extract to the glufosinate. *Fucus vesiculosus* extract is rich in *Fucus vesiculosus* polysaccharides and trace elements such as iodine, which are beneficial to the growth of some glufosinate-degrading microorganisms in the greenhouse soil, thus helping to degrade glufosinate and effectively reducing the occurrence of glufosinate phytotoxicity in greenhouses. Attached Figure Description

[0022] Figure 1 These are photos taken 3, 7, 14, and 22 days after application of the drug, in Example 2 of this invention.

[0023] Figure 2 These are photos of Comparative Example 1 of the present invention, taken 3, 7, 14, and 22 days after application of the drug.

[0024] Figure 3 These are photos of Comparative Example 2 of the present invention, taken 3, 7, 14, and 22 days after application of the drug.

[0025] Figure 4 These are photos of Comparative Example 3 of the present invention, taken 3, 7, 14, and 22 days after application of the drug.

[0026] Figure 5 These are photos of Comparative Example 4 of the present invention, taken 3, 7, 14, and 22 days after application of the drug. Detailed Implementation

[0027] The following embodiments can help those skilled in the art to more fully understand the present invention, but should not be construed as limiting the present invention in any way.

[0028] Example 1: 10% glufosinate aqueous solution

[0029] A synergistic glufosinate aqueous formulation comprises the following components by weight percentage:

[0030]

[0031] Preparation process: To prepare 1000g of the above-mentioned 10% glufosinate aqueous solution: In a 2000mL flask, add 812g of deionized water, 105g of glufosinate technical (content 95%), 1g of phycocyanin, 1g of adenosine triphosphate, 1g of cocamidopropyl betaine, and 60g of alkyl glycoside (APG). Stir evenly at 35℃ and 100r / min to obtain the first mixture. Then add 20g of sodium fatty alcohol polyoxyethylene ether sulfate (AES) and continue stirring for 20 minutes at 35℃ and 180r / min to obtain the 10% enhanced glufosinate aqueous solution.

[0032] Example 2: 20% glufosinate aqueous solution

[0033] A synergistic glufosinate aqueous formulation comprises the following components by weight percentage:

[0034]

[0035] Preparation process: To prepare 1000g of the above-mentioned 20% glufosinate aqueous solution: In a 2000mL flask, add 689g of deionized water, 210g of glufosinate technical (content 95%), 2g of phycocyanin, 2g of adenosine triphosphate, 50g of cocamidopropyl betaine, and 50g of alkyl glycoside (APG); stir evenly at 40℃ and 150r / min to obtain the first mixture; then continue stirring at 40℃ and 220r / min for 30 minutes to obtain the 20% enhanced glufosinate aqueous solution.

[0036] Example 3: 30% glufosinate aqueous solution

[0037] A synergistic glufosinate aqueous formulation comprises the following components by weight percentage:

[0038]

[0039] Preparation process: To prepare 1000g of the above-mentioned 30% glufosinate aqueous solution: In a 2000mL flask, add 561g of deionized water, 315g of glufosinate technical (content 95%), 2g of phycocyanin, 2g of adenosine triphosphate, 50g of cocamidopropyl betaine, and 50g of alkyl glycoside (APG). Stir evenly at 37℃ and 120r / min to obtain the first mixture. Then add 20g of sodium fatty alcohol polyoxyethylene ether sulfate (AES) and stir for 25 minutes at 37℃ and 200r / min to obtain the 30% enhanced glufosinate aqueous solution.

[0040] Example 4: 5% glufosinate aqueous solution

[0041] A synergistic glufosinate aqueous formulation comprises the following components by weight percentage:

[0042]

[0043]

[0044] Preparation process: To prepare 1000g of the above-mentioned 5% glufosinate aqueous solution: In a 2000mL flask, add 864.5g of deionized water, 52.5g of glufosinate technical (content 95%), 1g of phycocyanin, 1g of adenosine triphosphate, 1g of cocamidopropyl betaine, and 60g of alkyl glycoside (APG). Stir evenly at 38℃ and 130r / min to obtain the first mixture. Then add 20g of sodium fatty alcohol polyoxyethylene ether sulfate (AES) and continue stirring at 38℃ and 190r / min for 26 minutes to obtain the 5% enhanced glufosinate aqueous solution.

[0045] Example 5: 40% glufosinate aqueous solution

[0046] A synergistic glufosinate aqueous formulation comprises the following components by weight percentage:

[0047]

[0048] Preparation process: To prepare 1000g of the above-mentioned 40% glufosinate aqueous solution: In a 2000mL flask, add 496g of deionized water, 421g of glufosinate technical (content 95%), 1g of phycocyanin, 1g of adenosine triphosphate, 1g of cocamidopropyl betaine, and 60g of alkyl glycoside (APG). Stir evenly at 36℃ and 110r / min to obtain the first mixture. Then add 20g of sodium fatty alcohol polyoxyethylene ether sulfate (AES) and continue stirring at 36℃ and 190r / min for 27 minutes to obtain the 40% enhanced glufosinate aqueous solution.

[0049] Example 6: 40% glufosinate aqueous solution

[0050] A synergistic glufosinate aqueous formulation comprises the following components by weight percentage:

[0051]

[0052]

[0053] Preparation process: To prepare 1000g of the above-mentioned 40% glufosinate aqueous solution: In a 2000mL flask, add 488g of deionized water, 421g of glufosinate technical (content 95%), 1g of phycocyanin, 1g of adenosine triphosphate, 1g of cocamidopropyl betaine, and 60g of alkyl glycoside (APG). Stir evenly at 36℃ and 110r / min to obtain the first mixture. Then add 20g of sodium fatty alcohol polyoxyethylene ether sulfate (AES) and 8g of fucus vesiculosus extract. Continue stirring for 27 minutes at 36℃ and 190r / min to obtain the 40% enhanced glufosinate aqueous solution.

[0054] Example 7: 40% glufosinate aqueous solution

[0055] A synergistic glufosinate aqueous formulation comprises the following components by weight percentage:

[0056]

[0057] Preparation process: To prepare 1000g of the above-mentioned 40% glufosinate aqueous solution: In a 2000mL flask, add 483g of deionized water, 421g of glufosinate technical (content 95%), 1g of phycocyanin, 1g of adenosine triphosphate, 1g of cocamidopropyl betaine, and 60g of alkyl glycoside (APG). Stir evenly at 36℃ and 110r / min to obtain the first mixture. Then add 20g of sodium fatty alcohol polyoxyethylene ether sulfate (AES) and 13g of fucus vesiculosus extract. Continue stirring at 36℃ and 190r / min for 27 minutes to obtain the 40% enhanced glufosinate aqueous solution.

[0058] Example 8: 40% glufosinate aqueous solution

[0059] A synergistic glufosinate aqueous formulation comprises the following components by weight percentage:

[0060]

[0061]

[0062] Preparation process: To prepare 1000g of the above-mentioned 40% glufosinate aqueous solution: In a 2000mL flask, add 465g of deionized water, 421g of glufosinate technical (content 95%), 10g of phycocyanin, 10g of adenosine triphosphate, 1g of cocamidopropyl betaine, and 60g of alkyl glycoside (APG). Stir evenly at 36℃ and 110r / min to obtain the first mixture. Then add 20g of sodium fatty alcohol polyoxyethylene ether sulfate (AES) and 13g of fucus vesiculosus extract. Continue stirring at 36℃ and 190r / min for 27 minutes to obtain the 40% enhanced glufosinate aqueous solution.

[0063] Bioassay Validation: Validating the herbicidal activity of 20% glufosinate aqueous solution

[0064] Comparative Example 1: Contains no phycocyanin

[0065] 20% glufosinate aqueous solution (phycocyanin-free)

[0066] A synergistic glufosinate aqueous formulation comprises the following components by weight percentage:

[0067]

[0068] Preparation process: To prepare 1000g of the above-mentioned 20% glufosinate aqueous solution: In a 2000mL flask, add 691g of deionized water, 210g of glufosinate technical (content 95%), 2g of adenosine triphosphate, 50g of cocamidopropyl betaine, and 50g of APG in sequence; stir for 20-30 minutes to obtain the 20% enhanced glufosinate aqueous solution.

[0069] Comparative Example 2 (without adenosine triphosphate)

[0070] 20% glufosinate aqueous solution (adenosine triphosphate-free)

[0071] A synergistic glufosinate aqueous formulation comprises the following components by weight percentage:

[0072]

[0073] Preparation process: To prepare 1000g of the above-mentioned 20% glufosinate aqueous solution: In a 2000mL flask, add 691g of deionized water, 210g of glufosinate technical (content 95%), 2g of phycocyanin, 50g of cocamidopropyl betaine, and 50g of APG in sequence; stir for 20-30 minutes to obtain the 20% enhanced glufosinate aqueous solution.

[0074] Comparative Example 3 (without phycocyanin and adenosine triphosphate)

[0075] 20% glufosinate aqueous solution (free of phycocyanin and adenosine triphosphate)

[0076] A synergistic glufosinate aqueous formulation comprises the following components by weight percentage:

[0077]

[0078] Preparation process: To prepare 1000g of the above-mentioned 20% glufosinate aqueous solution: In a 2000mL flask, add 693g of deionized water, 210g of glufosinate technical (content 95%), 50g of cocamidopropyl betaine, and 50g of APG in sequence; stir for 20-30 minutes to obtain the 20% enhanced glufosinate aqueous solution.

[0079] Comparative Example 4 (without synergist)

[0080] Glufosinate 20%

[0081] Deionized water replenishment

[0082] Preparation process: To prepare 1000g of the above-mentioned 20% glufosinate aqueous solution: In a 2000mL flask, add 790g of deionized water and 210g of glufosinate technical (content 95%) in sequence, and stir for 20-30 minutes to obtain the 20% enhanced glufosinate aqueous solution.

[0083] Comparative Example 5

[0084] A synergistic glufosinate aqueous formulation comprises the following components by weight percentage:

[0085]

[0086] Preparation process: To prepare 1000g of the above-mentioned 40% glufosinate aqueous solution: In a 2000mL flask, add 475g of deionized water, 421g of glufosinate technical (content 95%), 10g of adenosine triphosphate, 1g of cocamidopropyl betaine, and 60g of alkyl glycoside (APG) in sequence. Stir evenly at 36℃ and 110r / min to obtain the first mixture. Then add 20g of sodium fatty alcohol polyoxyethylene ether sulfate (AES) and 13g of fucus vesiculosus extract. Continue stirring at 36℃ and 190r / min for 27 minutes to obtain the 40% enhanced glufosinate aqueous solution.

[0087] Comparative Example 6

[0088] A synergistic glufosinate aqueous formulation comprises the following components by weight percentage:

[0089]

[0090] Preparation process: To prepare 1000g of the above-mentioned 40% glufosinate aqueous solution: In a 2000mL flask, add 475g of deionized water, 421g of glufosinate technical (content 95%), 10g of phycocyanin, 1g of cocamidopropyl betaine, and 60g of alkyl glycoside (APG) in sequence. Stir evenly at 36℃ and 110r / min to obtain the first mixture. Then add 20g of sodium fatty alcohol polyoxyethylene ether sulfate (AES) and 13g of fucus vesiculosus extract. Continue stirring for 27 minutes at 36℃ and 190r / min to obtain the 40% enhanced glufosinate aqueous solution.

[0091] The following bioassay experiments demonstrate the efficacy of the glufosinate-ammonium aqueous solution provided in this embodiment of the invention.

[0092] Bioassay Experiment:

[0093] Experiment Title: Indoor Activity Verification Experiment of Glufosinate with Different Adjuvants on Annual Weeds in Non-Cultivated Land

[0094] Experimental method: Indoor potted plant foliar spraying method

[0095] Target weeds and leaf age: Digitaria sanguinalis at 3 leaves and 1 bud, Amaranthus retroflexus at 5-6 leaves.

[0096] nourish

[0097] Test targets: Digitaria sanguinalis and Amaranthus retroflexus were sown in small plastic seedling pots filled with topsoil collected from farmland, dried and sieved, and mixed with sphagnum moss (soil: sphagnum moss = 3:1). The organic matter content was 1.0%-1.4%, and the pH value was 7.0-7.5. The mixture was placed in cups with holes at the bottom, and water was poured in from the bottom to saturate the soil. Then, plump and uniform weed seeds were selected and evenly placed on the soil surface, and covered with 0.5cm of soil. Water was then naturally absorbed from the bottom of the pots. Finally, the pots were placed in a controlled greenhouse for cultivation. When the seedlings had one leaf and one bud, they were manually thinned with tweezers. When the target plants had 3-4 leaves, the final planting was carried out, keeping only the strongest and most uniform plants from each pot for later use. Watering was done daily depending on the dryness of the soil.

[0098] The cultivation conditions were: natural light 14h / 10h (L / D); cultivation temperature 7.90-53.80℃; relative humidity 50-75%. Target weeds and leaf age: Digitaria sanguinalis at 3 leaves and 1 bud; Amaranthus retroflexus at 5-6 leaves.

[0099] Used capacity

[0100] For foliar treatment, the water volume is 30 L / mu. A two-stage dilution method is used for pesticide preparation. Weigh the pesticide into 20 mL vials in sequence. Measure 300 mL of water. First, pour a small amount of water into the application bottle labeled with the treatment number. Dissolve and shake the weighed pesticide in several batches, then pour each batch into a 500 mL application bottle. Finally, pour the remaining water into the application bottle and shake it up and down for 10 seconds. Before application, ensure the sprayer is thoroughly rinsed and the bottle is shaken to ensure complete mixing before pouring it into the application equipment. After application, place the bottle in a sunny greenhouse for routine cultivation, and conduct regular experimental observations and target maintenance.

[0101] Experimental investigation and data analysis

[0102] Visual observation

[0103] Weed control efficacy survey

[0104] Visual observation: Visually observe changes in symptoms such as weed leaves and plant height, and compare with the blank control group. Regularly observe the symptoms of target weed poisoning and the subsequent recovery of the treatment groups after the application of the pesticide, and record them in detail.

[0105] Absolute value survey method: At the end of the experiment, a fresh weight survey is conducted. Surviving weeds are cut along the soil surface with scissors, and their fresh weight is measured using a balance. The plant control efficacy and fresh weight control efficacy of the tested herbicide against the weeds are calculated. The calculation formula is as follows:

[0106]

[0107] 6.2 Survey Time and Frequency

[0108] The efficacy test investigation was conducted at 1d, 2d, 4d, 6d, 10d, 14d, 18d, and 22d, for a total of 8 investigations.

[0109] Visual inspection

[0110] One day after the treatment, no obvious symptoms were observed in any of the treatments of Digitaria sanguinalis; however, Amaranthus retroflexus leaves showed signs of dehydration and the leaf edges were curled.

[0111] Two days after application, the leaves of all treatments of *Digitaria sanguinalis* turned yellow and dehydrated, with the leaf tips wilting. *Amaranthus retroflexus* leaves showed some signs of dehydration, with dry, curled leaf edges and chlorotic central leaves. Overall effect: Example 2: 20% glufosinate AS > Comparative Example 1: 20% glufosinate AS = Comparative Example 2: 20% glufosinate AS > Comparative Example 3: 20% glufosinate AS > Comparative Example 4: 20% glufosinate AS.

[0112] Four days after application, most leaves of the crabgrass in all treatments turned yellow and withered; the leaves of the amaranth turned yellow and withered in some areas.

[0113] Six days after application, most leaves of Crataegus pinnatifida in all treatments withered, the central leaves turned green and some withered; most leaves of Amaranthus retroflexus turned yellow and withered, the central leaves turned green and a few withered. Treatment Example 2 was the best.

[0114] Ten days after application, the treatments of Comparative Example 3 (20% glufosinate AS), Comparative Example 4 (20% glufosinate AS), and Crataegus pinnatifida began to turn green again, with a few leaves growing normally. The majority of stems in the other treatments died incompletely. Most leaves of Amaranthus retroflexus withered and died, while a few leaves and most stems died incompletely.

[0115] Fourteen days after application, in all treatments, *Amaranthus retroflexus* showed new shoots, while *Digitaria sanguinalis* did not die completely; the lower stems of the target weeds also did not die completely. Overall...

[0116] Example 2: 20% glufosinate AS > Comparative Example 1: 20% glufosinate AS = Comparative Example 2: 20% glufosinate AS > Comparative Example 3: 20% glufosinate AS > Comparative Example 4: 20% glufosinate AS.

[0117] 18 and 22 days after application, a few *Digitaria sanguinalis* and *Amaranthus retroflexus* plants revived, but most leaves withered and died, and a few stems died incompletely. Overall, Example 2: 20% glufosinate AS showed the best results.

[0118] Visual summary: Based on observation, Example 2 is the best. The control efficacy of Comparative Example 1 and Comparative Example 2 against the target weeds is not significantly different, but it is significantly better than Comparative Example 3 and Comparative Example 4.

[0119] Data Analysis

[0120] Table 1. Indoor activity verification test of glufosinate with different adjuvants against annual weeds in non-cultivated land - 22 days after application.

[0121]

[0122] Indoor biological testing showed that:

[0123] Based on the analysis, Example 2 was the best. The control efficacy of Comparative Example 1 and Comparative Example 2 against the target weeds was not significantly different, but it was significantly better than Comparative Example 3 and Comparative Example 4.

[0124] Further greenhouse experiments were conducted using Examples 5 (without Fucus vesiculosus extract) and Examples 6-8 (with Fucus vesiculosus extract).

[0125] The degradation rate of glufosinate was investigated, and the results are shown in Table 2 below.

[0126] Table 2

[0127]

[0128] Therefore, this invention provides an enhanced glufosinate aqueous solution and its preparation method. By adding phycocyanin, which promotes light energy absorption and transmission, to glufosinate, along with adenosine triphosphate (ATP), which promotes leaf respiration, and alkyl glycosides and cocamidopropyl betaine, which have wetting and spreading effects, the application significantly improves plant photosynthesis and respiration, facilitating glufosinate penetration through the cuticle and wax layer into weeds. This effectively enhances plant absorption of glufosinate, accelerates efficacy, and improves the low-temperature herbicidal activity of the glufosinate aqueous solution. Furthermore, this application adds an appropriate amount of *Fucus vesiculosus* extract to glufosinate. *Fucus vesiculosus* extract is rich in *Fucus vesiculosus* polysaccharides and trace elements such as iodine, which is beneficial to the growth of some microorganisms in greenhouse soil capable of degrading glufosinate. This helps the greenhouse soil degrade glufosinate, thereby effectively reducing the occurrence of glufosinate phytotoxicity in greenhouses.

[0129] Although the present invention has been described in detail above with general descriptions and specific embodiments, modifications or improvements can be made to it, which will be obvious to those skilled in the art. Therefore, all such modifications or improvements made without departing from the spirit of the present invention fall within the scope of protection claimed by the present invention.

Claims

1. A synergistic glufosinate aqueous solution, characterized in that: The enhanced glufosinate aqueous solution comprises the following components by weight percentage: 5-40% glufosinate active ingredient, 0.1-1% phycocyanin, 0.1-1% adenosine triphosphate (ATP), 0.1-10% alkyl glycoside, 0.1-10% cocamidopropyl betaine, 0.8-1.3 wt% Fucus vesiculosus extract, 0-5% anionic surfactant, and the balance being water; the weight ratio of phycocyanin to ATP is 1:

1.

2. The synergistic glufosinate aqueous solution according to claim 1, characterized in that: The enhanced glufosinate aqueous solution comprises the following components by weight percentage: 8-40% glufosinate active ingredient, 0.3-1% phycocyanin, 0.3-1% adenosine triphosphate (ATP), 0.8-10% alkyl glycoside, 0.5-10% cocamidopropyl betaine, 0.8-1.3 wt% fucus vesiculosus extract, 0.7-5% anionic surfactant, and the balance being water; the weight ratio of phycocyanin to ATP is 1:

1.

3. The synergistic glufosinate aqueous solution according to claim 1, characterized in that: The enhanced glufosinate aqueous solution comprises the following components by weight percentage: 10-40% glufosinate active ingredient, 0.5-1% phycocyanin, 0.5-1% adenosine triphosphate, 3-10% alkyl glycoside, 2-10% cocamidopropyl betaine, 0.8-1.3 wt% fucus vesiculosus extract, 2-5% anionic surfactant, and the balance being water; the weight ratio of phycocyanin to adenosine triphosphate is 1:

1.

4. The synergistic glufosinate aqueous solution according to claim 1, characterized in that: The anionic surfactant is sodium fatty alcohol polyoxyethylene ether sulfate, sodium ricinoleate sulfate, or sodium lauroyl sarcosinate.

5. The synergistic glufosinate aqueous solution according to claim 1, characterized in that: The weight percentage of the Fucus vesiculosus extract is 1.2 to 1.3 wt%.

6. A method for preparing a synergistic glufosinate aqueous solution according to any one of claims 1-5, characterized in that: Includes the following steps: (1) Material taking: Weigh each component of the synergistic glufosinate aqueous solution according to the weight percentage; (2) Preparation of the first mixture: Put the water obtained in step (1) into a container, and add glufosinate technical, phycocyanin, adenosine triphosphate, alkyl glycoside, and cocamidopropyl betaine in sequence; stir evenly to obtain the first mixture; (3) Mix and stir again: Add the remaining components to the first mixture and continue stirring for 20-30 minutes to obtain the enhanced glufosinate aqueous solution.

7. The method for preparing a synergistic glufosinate aqueous solution according to claim 6, characterized in that: In step (2), the water obtained in step (1) is placed in a container, and glufosinate technical, phycocyanin, adenosine triphosphate, alkyl glycoside, and cocamidopropyl betaine are added in sequence. The mixture is stirred evenly at a temperature of 35-40°C and a stirring speed of 100-150 r / min to obtain the first mixture.

8. The preparation method of the synergistic glufosinate aqueous solution according to claim 6, characterized in that: In step (3), the remaining components are added to the first mixture, and the mixture is stirred for 20 to 30 minutes at a temperature of 35 to 40°C and a stirring speed of 180 to 220 r / min to obtain the enhanced glufosinate aqueous solution.

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