A pesticide insecticide containing brofenac and fenthiocarb

Abstract

The present invention discloses a pesticide insecticide containing brofenac and fenthiocarb. The pesticide insecticide includes active ingredients, wherein the active ingredients are brofenac and fenthiocarb; the mass ratio of brofenac to fenthiocarb is 1:20 to 20:1. Due to the specific selection of brofenac and fenthiocarb for compounding, the two can produce a synergistic effect, thereby making the formed pesticide insecticide have good insecticidal effects and a wide control spectrum, especially for lepidopteran pests such as diamondback moth, gypsy moth, and dipteran Culex pipiens pallens.

Description

Technical Field

[0001] The invention relates to the technical field of pesticides, in particular to a pesticide insecticide containing brofenoxamide and methoprenaline. Background Art

[0002] Broflanilide is a diamide insecticide discovered by Mitsui Chemicals Agro Co., Ltd. and jointly developed by Mitsui Chemicals and BASF. Its mechanism of action is novel and completely different from that of o-diamide insecticides such as chlorantraniliprole. Broflanilide is an allosteric modulator of γ-aminobutyric acid (GABA)-gated chloride ion channels. It can be metabolized into demethylbroflanilide in insects, which is a non-competitive γ-aminobutyric acid (GABA) receptor antagonist with dieldrin (RDL). Broflanilide has no cross-resistance with other existing insecticides and can be used to It effectively controls pests resistant to other insecticides. Brofenac is highly effective, has a broad insecticide spectrum, acts quickly, and has a long-lasting effect. It is primarily used in fruit trees, vegetables, soybeans, cotton, corn, rice, grains, potatoes, and non-crop fields. It is highly effective against Lepidoptera (diamondback moth, Spodoptera litura, Spodoptera exigua, cotton bollworm, fall armyworm, etc.), Coleoptera (yellow flea beetle, monkey leaf beetle, etc.), and Thrips (palm thrips, onion thrips, tobacco thrips, etc.). Seed treatment can control grain wireworms and is also used to control sanitary pests such as termites, ants, cockroaches, flies, and mosquitoes. Its structural formula is as follows:

[0003]

[0004] Dimethoprim is a pyrazole amide compound, its English common name is dimpropyridaz; its trade name is Axalion TM IUPAC name: 1-[(1RS)-1,2-dimethylpropyl]-N-ethyl-5-methyl-N-pyridazin-4-yl-1H-pyrazole-4-carboxamide; CAS registration number: 1403615-77-9; molecular formula: C 16 H 23 N5O; Mefenoxam has excellent performance and good systemic conductivity. It is mainly used for field crops such as fruit trees and vegetables, soybeans, other legumes, cotton, cereals, potatoes, as well as flowers and ornamental plants. It controls pests such as Lepidoptera (striped stem borer, beet armyworm, diamondback moth, fall armyworm, etc.), Coleoptera (beetles, corn root leaf beetle, potato leaf beetle, yellow flea beetle, etc.), Diptera (flies, mosquitoes, vegetable leafminer, etc.), Hemiptera (aphids, planthoppers, psyllids, whiteflies, etc.), Thysanoptera (thrips, orchid thrips, palm thrips, tobacco thrips, etc.), Isoptera (termites, etc.), cockroaches, and ants. It is particularly effective against piercing-sucking pests such as aphids, whiteflies, and psyllids. Its structural formula is shown below:

[0005]

[0006] Due to the vast areas of vegetable cultivation and the rapid reproduction of the lepidopteran pest Plutella xylostella, its control is difficult and its damage is severe. Furthermore, the widespread distribution of forestry makes the control of the gypsy moth, a forestry quarantine pest, particularly challenging. Furthermore, the resistance of dipteran pests such as Culex pipiens and houseflies is increasing year by year. Currently, commonly used control methods, such as emamectin benzoate for lepidopteran pests and pyrethroid compounds such as lambda-cyhalothrin for dipteran pests, are outdated and have a high resistance rate, resulting in poor control effectiveness.

[0007] Therefore, existing technologies combine ingredients with different mechanisms of action to prevent pest resistance. However, after compounding, whether the compounding is synergistic, additive, or antagonistic requires evaluation based on actual application results. Typically, these compounding formulas exhibit antagonistic effects, while additive effects are rare. Even fewer exhibit significant synergistic effects and high co-toxicity coefficients. Therefore, providing a compounding formula with excellent pest control efficacy is an urgent problem in the prior art. Summary of the Invention

[0008] In view of the above problems, the present invention provides a pesticide containing brofenoxamide and anthracene with excellent control effect, which overcomes the above problems or at least partially solves the above problems, and can solve the problem in the prior art that lepidopteran pests and dipteran pests are prone to resistance and difficult to control.

[0009] Specifically, the present invention provides a pesticide insecticide containing brofenac and methoprene, wherein the pesticide insecticide includes active ingredients, wherein the active ingredients are brofenac and methoprene; the mass ratio of brofenac to methoprene is 1:20 to 20:1.

[0010] Optionally, it is characterized in that the mass ratio of the brofenac to the anthracene is 15:1 to 1:5.

[0011] Optionally, the mass ratio of the brofenac to the anthracene is 5:1.

[0012] Optionally, the mass ratio of the brofenac to the anthracene is 10:1 to 1:10.

[0013] Optionally, the mass ratio of the brofenac to the anthracene is 1:1.

[0014] Optionally, the ratio of the mass of the brofenac to the mass of the pesticide is 1-20%.

[0015] Optionally, the ratio of the mass of the thiamethoxam to the mass of the pesticide is 1-20%.

[0016] Optionally, the pesticide is used to control lepidopteran pests; the lepidopteran pests include at least the diamondback moth, the gypsy moth, the beet armyworm, the leaf armyworm, the striped stem borer and the fall armyworm.

[0017] Optionally, the pesticide is used to control dipteran pests; the dipteran pests include at least Culex pipiens pallens, Aedes albopictus and housefly.

[0018] Optionally, the pesticide is in the form of a liquid preparation.

[0019] The beneficial effects of the present invention are:

[0020] The pesticide provided by the present invention contains brofenac and fenthiocarb. Since brofenac and fenthiocarb are specifically selected for compounding, the two can produce a synergistic effect, thereby making the formed pesticide insecticide have good insecticidal effects and a wide control spectrum, especially for lepidopteran pests such as the diamondback moth and the gypsy moth, and dipteran pests such as the Culex pipiens.

[0021] Furthermore, when the mass ratio of brofenac to methoprene was 20:1-1:20, the combination showed a synergistic effect against Plutella xylostella. In particular, when the mass ratio of brofenac to methoprene was 5:1, the CTC value was 170.62, showing a significant synergistic effect.

[0022] Furthermore, when the mass ratio of brofenac to methoprene was 20:1-1:15, the combination showed a synergistic effect against the nymphal moth. In particular, when the mass ratio of brofenac to methoprene was 5:1, the CTC value was 148.86, showing a significant synergistic effect.

[0023] Furthermore, when the mass ratio of brofenac to methoprene was 20:1-1:20, the combination showed a synergistic effect against Culex pipiens pallens. In particular, when the mass ratio of brofenac to methoprene was 1:1, the CTC value was 168.78, showing a significant synergistic effect. DETAILED DESCRIPTION

[0024] To make the purpose, technical solutions and advantages of the embodiments of the present invention more clear, the technical solutions of the embodiments of the present invention are described clearly and completely below. Where specific conditions are not specified in the embodiments, conventional conditions or conditions recommended by the manufacturer shall be followed.

[0025] Due to the vast areas of vegetable cultivation and the rapid reproduction of the lepidopteran pest Plutella xylostella, its control is challenging and its damage is severe. Furthermore, the widespread distribution of forestry makes the control of the gypsy moth, a forestry quarantine pest, particularly challenging. Furthermore, the resistance of dipteran pests such as Culex pipiens and houseflies is increasing year by year. Currently, commonly used control methods, such as emamectin benzoate for lepidopteran pests and pyrethroids such as lambda-cyhalothrin for dipteran pests, involve older, more resistant pesticides, resulting in poor control effectiveness. Publicly available reports have not yet fully investigated the activity of brofenac and fenthiocarb against Plutella xylostella, the gypsy moth, and Culex pipiens.

[0026] An embodiment of the present invention provides a pesticide containing brofenac and thiamethoxam, wherein the pesticide comprises an active ingredient consisting of brofenac and thiamethoxam, wherein the mass ratio of brofenac to thiamethoxam is 1:20 to 20:1.

[0027] The embodiment of the present invention specifically selects bromofenac and fenthiocarb for compounding, and the two can produce a synergistic effect, thereby making the formed pesticide insecticide have good insecticidal effect and a large control spectrum, especially for lepidopteran pests such as diamondback moth, gypsy moth, etc.; Diptera pests such as Culex pipiens pallens, with good control effect. It should be noted that the pesticide insecticide provided by the present invention is used to control insect pests of crops including but not limited to lepidopteran pests such as diamondback moth, gypsy moth, etc., Diptera pests such as Culex pipiens pallens, etc., but the pesticide insecticide has a better control effect on lepidopteran pests such as diamondback moth, gypsy moth, and Diptera pest Culex pipiens pallens; in addition, the pesticide insecticide does not affect the normal growth of plants.

[0028] Specifically, the mass ratio of brofenac to methoprene is 1:20, 1:15, 1:10, 1:5, 1:1, 5:2, 5:1, 10:1, 15:1, or 20:1. Preferably, the mass ratio of brofenac to methoprene is 5:1 or 1:1.

[0029] Furthermore, the pesticides and insecticides also include dispersants, thickeners, preservatives, and antifreeze agents. Dispersants include, but are not limited to, surfactants such as white carbon black, fatty alcohol polyoxyethylene ethers, polycarboxylates, and block polyether emulsifiers; thickeners include, but are not limited to, existing thickeners such as xanthan gum; preservatives include, but are not limited to, existing preservatives such as potassium sorbate; and antifreeze agents include, but are not limited to, existing antifreeze agents such as ethylene glycol.

[0030] The pesticide insecticide is a liquid preparation, preferably a suspension concentrate. It should be noted that the embodiments of the present invention only list suspension concentrates, but it is understandable that other types of liquid preparations are also within the scope of protection of the embodiments of the present invention.

[0031] The features and performance of the present invention are further described in detail below with reference to specific embodiments.

[0032] Example 1: 18% bromofenac·pyrimidine suspension (5:1)

[0033] 15.0 g of brofenac, 3.0 g of thiamethoxam, 2.5 g of alkylnaphthalene formaldehyde condensate sulfonate (dispersant), 1.5 g of isomeric alcohol polyoxyethylene ether (wetting agent), 0.3 g of xanthan gum, 5.0 g of ethylene glycol, and 0.5 g of potassium sorbate (preservative) are mixed, the mixture is made up to 100 g with deionized water, and then sand-milled with a sand mill to a particle size of less than 5 μm to obtain a pesticide insecticide.

[0034] Example 2: 18% bromofenac·pyrimidine suspension concentrate (1:1)

[0035] 9.0 g of brofenac, 9.0 g of thiamethoxam, 2.0 g of alkylnaphthalene formaldehyde condensate sulfonate (dispersant), 1.0 g of isomeric alcohol polyoxyethylene ether (wetting agent), 0.4 g of xanthan gum, 5.5 g of ethylene glycol, and 0.6 g of potassium sorbate (preservative) are mixed, the mixture is made up to 100 g with deionized water, and then sand-milled with a sand mill to a particle size of less than 5 μm to obtain a pesticide insecticide.

[0036] Comparative Example 1: 15% bromofenac suspension concentrate (15%)

[0037] 15.0 g of brofenac, 1.3 g of alkylnaphthalene formaldehyde condensate sulfonate (dispersant), 1.0 g of isomeric alcohol polyoxyethylene ether (wetting agent), 0.3 g of xanthan gum, 4.5 g of ethylene glycol, and 0.4 g of potassium sorbate (preservative) are mixed, the mixture is made up to 100 g with deionized water, and then sand-milled with a sand mill to a particle size of less than 5 μm to obtain a pesticide insecticide.

[0038] Comparative Example 2: 3% sulfamethoxam suspension concentrate (3%)

[0039] 3.0 g of thiamethoxam, 1.2 g of alkylnaphthalene formaldehyde condensate sulfonate (dispersant), 0.8 g of isomeric alcohol polyoxyethylene ether (wetting agent), 0.4 g of xanthan gum, 4.0 g of ethylene glycol, and 0.3 g of potassium sorbate (preservative) are mixed, the mixture is made up to 100 g with deionized water, and then sand-milled to a particle size of less than 5 μm to obtain a pesticide insecticide.

[0040] The pesticides and insecticides provided in the above examples and comparative examples were used in the following experimental examples to verify the toxicity and efficacy of the pesticides and insecticides.

[0041] Experimental Example 1 Indoor toxicity determination of compound pesticides against diamondback moth

[0042] According to the leaf dip method in Part 14 of the Guidelines for Indoor Bioassay of Pesticides NY / T1154.14-2008, the control effects of different mass ratios of brofenac and fenthiocarb on the lepidopteran pest Plutella xylostella were determined to determine the optimal mixing ratio of the two insecticides.

[0043] Lepidopteran pests, Plutella xylostella (Ustilaginoidea oryzae), were provided by the College of Plant Medicine, Qingdao Agricultural University. The mixing ratios of brofenac: methoprene were: 20:1; 15:1; 10:1; 5:1; 1:1; 1:5; 1:10; 1:15; and 1:20. The prepared concentrations are shown in Table 1:

[0044] Table 1 The dosage ratio of each substance in the insecticide composition

[0045]

[0046] The test method is the leaf immersion method: based on the preliminary test, 6 concentration gradients are set for each agent, with clean water as a blank control, and repeated 4 times, with 24 3-instar diamondback moth larvae used for each repetition. Use a hole punch to punch the clean cabbage leaves into discs with a diameter of about 2 cm, immerse the leaves in the corresponding agent treatment concentration, take them out after 10 seconds, dry them naturally and place them in a 12-well plate, put 1 piece in each well and inoculate 2 diamondback moth larvae that have been starved for 4 hours, and cover the 12-well plate with a layer of paper (to prevent the diamondback moth larvae from escaping). The experiment was carried out in the insect breeding room, and the death of the test insects was observed for 72 hours. Gently touch the insect body with a brush, and those that do not move are considered dead. Use DPS software as a statistical tool to calculate the toxicity regression equation and LD 50 .

[0047]

[0048]

[0049] When the mortality rate of the blank control test is <5%, no correction is required. When the mortality rate is between 5% and 20%, correction is performed according to the calibration mortality rate formula. When the mortality rate is >20%, the test is repeated.

[0050] According to the survey data, the mortality rate and corrected mortality rate of each treatment were calculated, and the data were processed using DPS software to calculate the LD of each agent. 50 LD 90 , b value (standard error) and LD 50 The 95% confidence limit of the mixture was calculated according to the Sun Yunpei method.

[0051] The co-toxicity coefficient (CTC value) of the mixture is calculated according to formula (1), formula (2), and formula (3):

[0052]

[0053] Where: ATI—measured toxicity index of mixture;

[0054] S—LD of standard pesticide 50 , the unit is milligrams per liter (mg / L);

[0055] M—LD of the mixture 50 , the unit is milligrams per liter (mg / L).

[0056] TTI=A×P A +B×P A (2)

[0057] Where: TTI—theoretical toxicity index of mixture;

[0058] A—A-agent toxicity index;

[0059] P A —The percentage of agent A in the mixture, expressed as percentage (%);

[0060] B—B agent toxicity index;

[0061] P B —The percentage of agent B in the mixture, in percentage (%).

[0062]

[0063] Where: CTC—co-toxicity coefficient; ATI—measured toxicity index of mixture; TTI—theoretical toxicity index of mixture.

[0064] A co-toxicity coefficient (CTC) of the combination ≥120 indicates a synergistic effect; CTC ≤80 indicates an antagonistic effect; and 80 < CTC <120 indicates an additive effect.

[0065] The test results are shown in Table 2:

[0066] Table 2 Results of the combined toxicity test of brofenac and anthracene to Plutella xylostella

[0067]

[0068] The test results are shown in Table 2: LD50 of brofenac and fenthiocarb technical against Plutella xylostella after 72 hours 50The values ​​were 0.0563 mg / L and 12.1020 mg / L, respectively; that is, compared with the original drug of fenthiocarb, the original drug of brofenthiocarb has higher stomach poison activity against the diamondback moth. After the original drug of brofenthiocarb and fenthiocarb are compounded, they show a synergistic combined effect on the diamondback moth; when the mass ratio of brofenthiocarb to fenthiocarb is 20:1-1:20, the compounded agent shows a synergistic effect on the diamondback moth. In particular, when the mass ratio of brofenthiocarb to fenthiocarb is 5:1 (the same mass ratio of brofenthiocarb to fenthiocarb in Example 1), the CTC value is 170.62, which has a significant synergistic effect.

[0069] Experimental Example 2: Field efficacy test on the control of cabbage diamondback moth

[0070] The pesticides and insecticides provided in Example 1 and Comparative Examples 1 and 2 were used to control Plutella xylostella in cabbage fields.

[0071] 1. Test process

[0072] The test agents are shown in Table 3:

[0073] Table 3 Field test dosage for controlling cabbage diamondback moth

[0074]

[0075] The experimental site is located in Guojiazhuang Village, Jimo District, Qingdao City (36.437345°N, 120.5631°E). The experimental plot covers an area of ​​1.5 mu (approximately 1.5 acres), with a soil pH of 6.4 and an organic matter content of 1.8%. The experimental plot is located in the northern subtropical monsoon climate zone, characterized by abundant sunlight, a mild climate, distinct seasons, moderate rainfall, and flat terrain, offering excellent irrigation and drainage conditions, making it suitable for the experiment.

[0076] Sowing Information: Cabbage seeds were purchased from the market, sown and raised on July 22, 2022, and transplanted on August 30, 2022. The planting density was approximately 3,500 plants per mu, with a plant spacing of approximately 40 cm and a row spacing of approximately 50 cm. Cultivation and management conditions were in line with local agricultural production practices. Fertilization Information: On August 30, 2022, approximately 50 kg of compound fertilizer (≥54%) was used per mu as base fertilizer.

[0077] Application time and growth period: The application time is September 18, 2022, when cabbage is in the seedling stage and diamondback moth is in the peak egg hatching period. The water consumption for application is 600L / hm2. 2 .

[0078] Control effectiveness survey: A five-point sampling method was used within each plot, with four cabbage plants randomly surveyed at each point, for a total of 20 cabbage plants per plot. Insect populations were recorded before application and three and seven days after application, and the population reduction rate and control effectiveness were calculated.

[0079] Efficacy calculation method: EXCEL was used to calculate the disease index and control efficacy, and the Duncan new multiple range (DMRT) method was used to perform variance analysis on the test data using DPS9.5 software.

[0080]

[0081]

[0082] 2. Test results

[0083] The control effects of different pesticides on the lepidopteran pest diamondback moth are shown in Table 4:

[0084] Table 4 Control effects of different pesticides on Plutella xylostella

[0085]

[0086] As shown in Table 4, the control efficacy survey results show that after 3 days of application, the control efficacy of Example 1 was 88.98%, which was significantly better than the control efficacy of Comparative Example 1 and Comparative Example 2 (84.41% and 82.15% respectively). After 7 days of application, the control efficacy of Example 1 was 96.52%, which was significantly better than the control efficacy of Comparative Example 1 and Comparative Example 2 (92.31% and 90.01% respectively). Field observations showed that both the test and control agents were safe for the test cabbage crop, and no symptoms of phytotoxicity (such as stunting, chlorosis, and deformity) were observed.

[0087] Experimental Example 3 Indoor toxicity determination of compound pesticides against the American moth

[0088] The determination method refers to NY / T 1154.10-2008 Guidelines for Indoor Bioassay Tests of Pesticides - Insecticides Part 10: Artificial Diet Mixture Method; the control effects of different mass ratios of brofenac and fenthiocarb on the lepidopteran pest, the gypsy moth, were determined to determine the optimal mixing mass ratio of the two insecticides.

[0089] The lepidopteran pest Hyphantria cunea was provided by the College of Plant Medicine, Qingdao Agricultural University. The mixing ratios of brofenac: anthracene were: 20:1; 15:1; 10:1; 5:1; 1:1; 1:5; 1:10; 1:15; and 1:20. The prepared concentrations are shown in Table 5:

[0090] Table 5 Dosage ratio of each substance in the insecticide composition

[0091]

[0092] Artificial feed mixing method: 2 mL of the prepared drug solution of varying concentrations was evenly mixed into the prepared artificial feed (with an organic solvent content of no more than 1%). The mixture was then poured into 12-well plates and cooled for later use. A control group was treated with a DMF diluent at the same DMF concentration as the other drug treatments. After 5 minutes, the feed was placed in an insect box. Each concentration was repeated three times, with 20 third-instar nymphalids of the cuneiform moth placed in each box. The boxes were then placed in the insectary and observed for nymphal mortality. The nymphs were gently poked with tweezers; if they did not respond to external stimuli, they were considered dead. After 72 hours, the number of deaths was counted and the adjusted mortality rate was calculated.

[0093]

[0094]

[0095] When the mortality rate of the blank control test is <5%, no correction is required. When the mortality rate is between 5% and 20%, correction is performed according to the calibration mortality rate formula. When the mortality rate is >20%, the test is repeated.

[0096] According to the survey data, the mortality rate and corrected mortality rate of each treatment were calculated, and the data were processed using DPS software to calculate the LD of each agent. 50 LD 90 , b value (standard error) and LD 50 The 95% confidence limit of the mixture was calculated according to the Sun Yunpei method.

[0097] The co-toxicity coefficient (CTC value) of the mixture is calculated according to formula (4), formula (5), and formula (6):

[0098]

[0099] Where: ATI—measured toxicity index of mixture;

[0100] S—LD of standard pesticide 50 , the unit is milligrams per liter (mg / L);

[0101] M—LD of the mixture 50 , the unit is milligrams per liter (mg / L).

[0102] TTI=A×P A +B×P A (5)

[0103] Where: TTI—theoretical toxicity index of mixture;

[0104] A—A-agent toxicity index;

[0105] P A—The percentage of agent A in the mixture, expressed as percentage (%);

[0106] B—B agent toxicity index;

[0107] P B —The percentage of agent B in the mixture, in percentage (%).

[0108]

[0109] Where: CTC—co-toxicity coefficient; ATI—measured toxicity index of mixture; TTI—theoretical toxicity index of mixture.

[0110] A co-toxicity coefficient (CTC) of the combination ≥120 indicates a synergistic effect; CTC ≤80 indicates an antagonistic effect; and 80 < CTC <120 indicates an additive effect.

[0111] The test results are shown in Table 6:

[0112] Table 6 Results of the combined toxicity test of brofenac and anthracene to the gypsy moth

[0113]

[0114] As shown in Table 6, the LD50 of brofenac and fenthiocarb technical against the American gypsy moth at 72 hours 50 The values ​​were 1.213 mg / L and 18.235 mg / L, respectively. This means that compared to the technical form of methoprene, brofenac technical has higher stomach toxicity against the gypsy moth. The combination of brofenac technical and methoprene technical exhibited a synergistic effect against the gypsy moth. When the mass ratio of brofenac to methoprene was 20:1-1:15, the combination exhibited a synergistic effect against the gypsy moth. In particular, when the mass ratio of brofenac to methoprene was 5:1, the CTC value reached 148.86, demonstrating a significant synergistic effect.

[0115] Experimental Example 4: Efficacy test of pesticides for controlling gypsy moth in forestry

[0116] The insecticide compositions provided in Example 1 and Comparative Examples 1 and 2 were used to control the gypsy moth in forestry.

[0117] 1. Test method

[0118] The test agents are shown in Table 7:

[0119] Table 7 Dosage table for field trials against the gypsy moth

[0120]

[0121] The experimental site is Yangqiao Village, Zhihe Township, Yongqiao District, Suzhou City, Anhui Province (longitude: 117.164501, latitude: 33.931301); planting conditions: Poplars were planted in 2018, with a row spacing of 3m×3m and a planting density of about 80 trees per mu. The poplars were of similar age, grew uniformly, and were managed in a standardized manner, in line with local agricultural production practices.

[0122] Application time and growth period: The application time is May 18, 2022, when the poplar trees are in the growth period and the 2nd to 3rd instar larvae of the poplar gypsy moth occur. The water consumption for application is 750L / hm2. 2 .

[0123] Investigation on the effect of prevention and control: In each plot, two trees were surveyed, and samples were taken at five points in the east, west, south, north and center of each tree. All the gypsy moth larvae on five branches were surveyed at each point. The number of insects was surveyed and recorded before, and 3 and 7 days after application, and the insect population reduction rate and prevention effect were calculated.

[0124] Efficacy calculation method: EXCEL was used to calculate the disease index and control efficacy, and the Duncan new multiple range (DMRT) method was used to perform variance analysis on the test data using DPS9.5 software.

[0125]

[0126]

[0127] 2. Test results

[0128] The control effects of different pesticides on the lepidopteran pest, the gypsy moth, are shown in Table 8:

[0129] Table 8 Control effects of different pesticides on poplar gypsy moth

[0130]

[0131] As shown in Table 8, the results of the control efficacy survey showed that after 3 days of application, the control efficacy of Example 1 was 87.21%, which was significantly better than the control efficacy of 83.32% and 80.10% of Comparative Example 1 and Comparative Example 2, respectively. After 7 days of application, the control efficacy of Example 1 was 90.90%, which was significantly better than the control efficacy of 86.67% and 84.23% of Comparative Example 1 and Comparative Example 2, respectively. Field observations showed that both the test agent and the control agent were safe for the test poplar trees, with no symptoms of phytotoxicity observed.

[0132] Experimental Example 5 Indoor toxicity determination of compound pesticides against Culex pipiens pallens

[0133] According to the stomach poison method, the effects of different mass ratios of brofenac and fenpyrad mixed on dipteran pests such as Culex pipiens pallens were measured to determine the optimal mixing mass ratio of the two insecticides.

[0134] The dipteran pest Culexpipiens pallens was provided by the Nanjing Military Region Disease Control Research Center. The mixing ratios of brofenac: methoprene were: 20:1; 15:1; 10:1; 5:1; 1:1; 1:5; 1:10; 1:15; and 1:20. The prepared concentrations are shown in Table 9:

[0135] Table 9 The dosage ratio of each substance in the insecticide composition

[0136]

[0137]

[0138] Test method: The stomach poison method was used. On the basis of the preliminary test, 6 concentration gradients were set for each agent. Clean water was used as a blank control. The test was repeated 4 times. Each repetition used 30 female Culex pipiens pipiens that had emerged for 3 days and had not sucked blood. The technical drugs of brofenac and fenthiocarb were dissolved in acetone, and distilled water was added to prepare the mother liquor of the required concentration. The above mother liquors were mixed in a certain proportion to prepare a mixed mother liquor, which was diluted into a series of concentrations. The dilution was prepared into 5% drug-containing sugar water. Stomach poison method: Culex pipiens pipiens were fed with drug-containing sugar water, raised in a standard manner, and the test was repeated 4 times in the insect breeding room to observe the death of the test insects for 24 hours. The insect body was gently touched with a brush, and those that did not move were considered dead. DPS software was used as a statistical tool to calculate the toxicity regression equation and LD 50 .

[0139]

[0140]

[0141] When the mortality rate of the blank control test is <5%, no correction is required. When the mortality rate is between 5% and 20%, correction is performed according to the calibration mortality rate formula. When the mortality rate is >20%, the test is repeated.

[0142] According to the survey data, the mortality rate and corrected mortality rate of each treatment were calculated, and the data were processed using DPS software to calculate the LD of each agent. 50 LD 90 , b value (standard error) and LD 50 The 95% confidence limit of the mixture was calculated according to the Sun Yunpei method.

[0143] The co-toxicity coefficient (CTC value) of the mixture is calculated according to formula (7), formula (8), and formula (9):

[0144]

[0145] Where: ATI—measured toxicity index of mixture;

[0146] S—LD of standard pesticide 50 , the unit is milligrams per liter (mg / L);

[0147] M—LD of the mixture 50 , the unit is milligrams per liter (mg / L).

[0148] TTI=A×P A +B×P A (8)

[0149] Where: TTI—theoretical toxicity index of mixture;

[0150] A—Agent toxicity index;

[0151] P A —The percentage of agent A in the mixture, expressed as percentage (%);

[0152] B—B agent toxicity index;

[0153] P B —The percentage of agent B in the mixture, in percentage (%).

[0154]

[0155] Where: CTC—co-toxicity coefficient; ATI—measured toxicity index of mixture; TTI—theoretical toxicity index of mixture.

[0156] A co-toxicity coefficient (CTC) of the combination ≥120 indicates a synergistic effect; CTC ≤80 indicates an antagonistic effect; and 80 < CTC <120 indicates an additive effect.

[0157] The test results are shown in Table 10:

[0158] Table 10 Results of the combined toxicity test of brofenac and anthracene to Culex pipiens pallens

[0159]

[0160] As shown in Table 10, the stomach toxicity LD of brofenac and fenthiocarb technical against Culex pipiens pallens at 72h 50The values ​​were 0.121 mg / L and 0.204 mg / L, respectively. This indicates that compared to methoprene technical, brofenac technical has higher stomach toxicity against Culex pipiens pallens. The combination of brofenac technical and methoprene technical exhibited a synergistic effect against Culex pipiens pallens. When the mass ratio of brofenac to methoprene was between 20:1 and 1:20, the combination exhibited a synergistic effect against Culex pipiens pallens. In particular, when the mass ratio of brofenac to methoprene was 1:1, the CTC value reached 168.78, demonstrating significant synergistic activity.

[0161] Experimental Example 6: Efficacy test for controlling Culex pipiens pallens

[0162] The pesticides and insecticides provided in Example 2 and Comparative Examples 1 and 2 were used to control Culex pipiens pallens.

[0163] 1. Test method

[0164] The test was conducted in Jimo District, Qingdao City. The test method used hedgerow technology, spraying pesticides on the surface of shrubs, lawns, tree trunks, ornamental flowers and trees around the buildings in the test area. The treatment area was the area below 5 meters of the vegetation, focusing on the downward surface of leaves. This was repeated three times. A blank control group was set up, mosquito and egg traps were used, and monitoring was carried out once a week.

[0165] Evaluation index: insect and egg attraction index, the formula is as follows:

[0166]

[0167] The recovered mosquito and ovitraps contain adult mosquitoes and / or mosquito eggs, which are positive mosquito and ovitraps.

[0168] 2. Test results

[0169] The control effects of different pesticides on the dipteran pest Culex pipiens pallens are shown in Table 11:

[0170] Table 11 On-site control effects of different pesticides on Culex pipiens pallens

[0171]

[0172] According to Table 11, the control rate and duration of the 18% brofenac·methopamide suspension hedge technology for on-site control of Culex pipiens pallens were much higher than those of single-agent brofenac and single-agent methopamide.

[0173] At this point, those skilled in the art will recognize that, although a number of exemplary embodiments of the present invention have been shown and described in detail herein, many other variations or modifications consistent with the principles of the present invention may be directly determined or derived from the disclosure of the present invention without departing from the spirit and scope of the present invention. Therefore, the scope of the present invention should be understood and deemed to cover all such other variations or modifications.

Claims

1. A pesticide insecticide containing brofenoxamide and fenthiocarb for controlling lepidopteran and dipteran pests, characterized in that: The invention comprises active ingredients, wherein the active ingredients are brofenac and thiamethoxam; the mass ratio of brofenac to thiamethoxam is (20-5):1, 1:(5-15); the lepidopteran pests are diamondback moth and gypsy moth; and the dipteran pest is Culex pipiens pallens.

2. The use according to claim 1, characterized in that The mass ratio of the brofenac to the anthracenamide is 5:

1.

3. The use according to claim 2, characterized in that The ratio of the mass of the brofenac to the mass of the pesticide is 15%.

4. The use according to claim 2, characterized in that The ratio of the mass of the thiamethoxam to the mass of the pesticide is 3%.

5. The use according to any one of claims 1 to 4, characterized in that The dosage form of the pesticide is a liquid preparation.

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