Unmanned aerial vehicle aerial inoculation of dry powder fungicide

Abstract

The application provides a method for unmanned aerial vehicle aerial seeding of dry powder fungicide, comprising the following steps: pretreating prepared dry powder fungicide to obtain dry powder fungicide particles; preparing aerial seeding balls: the surface of the aerial seeding ball is provided with a plurality of micro-pore groups, each micro-pore group is composed of 3-6 micro-pores, and the openings of the plurality of micro-pores in each micro-pore group are distributed equidistantly on a same circle; mixing the dry powder fungicide particles and the aerial seeding ball, so that the dry powder fungicide particles are filled in the micro-pores, to prepare dry powder fungicide balls for unmanned aerial vehicle aerial seeding, and the mass of the dry powder fungicide particles filled in the dry powder fungicide ball per unit mass is calculated; according to the mass of the dry powder fungicide particles filled in the dry powder fungicide ball per unit mass, and in combination with the mass of the dry powder fungicide required by the aerial seeding area, the total mass of the dry powder fungicide balls required for aerial seeding is calculated, and then unmanned aerial vehicle aerial seeding is performed. The operation of the application is convenient and fast, and solves the problem of low operability of seeding a small amount of dry powder fungicide per unit area.

Description

Technical Field

[0001] This invention relates to the field of drone aerial seeding technology, and more specifically, to a method for drone aerial seeding inoculation with dry powder microbial agents. Background Technology

[0002] Dark septate endophytes (DSEs) refer to a class of small soil fungi that colonize the intercellular spaces or interiors of plant root cells. They typically form septate hyphae and microsclerotia within the epidermis, cortex, and intercellular spaces of healthy plant roots, exhibiting a wide ecological distribution and even existing in the root systems of non-mycorrhizal plants. Many studies have shown that DSEs have similar ecological functions to arbuscular mycorrhizal fungi (AMFs), and their symbiotic relationship with plants can enhance the plant's tolerance to stress. Furthermore, DSEs are characterized by rapid cultivation; some studies have prepared DSE inoculum into dry powder products, further increasing the ease of transportation and reducing costs.

[0003] However, DSE dry powder microbial agent is a concentrated product, requiring only a small application rate to achieve efficient fertilizer substitution. However, its application presents certain challenges: direct manual application is labor-intensive and difficult to execute. Even dissolving DSE dry powder in water for spraying still requires significant manpower. While drone spraying technology has improved efficiency and reduced manpower to some extent, DSE dry powder cannot be directly sprayed by drones. This is primarily because the small dosage and low density of the dry powder make it easily blown away by the wind or drift away, making it difficult to achieve the intended target. Drone spraying of DSE dry powder aqueous solutions also presents problems; in areas with vegetation, the sprayed liquid is trapped by the vegetation and cannot directly reach the ground to exert its effect. Therefore, the method of inoculating with dry powder microbial agents is a limiting factor restricting the large-scale, routine, and intensive use of these agents. Summary of the Invention

[0004] This invention provides a method for aerial seeding of dry powder microbial agents by drones, which improves the efficiency and operability of inoculating dry powder microbial agents in soil.

[0005] To address the above problems, this invention provides a method for aerial seeding and inoculation of dry powder microbial agents using unmanned aerial vehicles, comprising the following steps:

[0006] S10. Pre-treat the prepared dry powder bacterial agent to obtain dry powder bacterial agent granules;

[0007] S20. Preparation of aerial seeding ball: The surface of the aerial seeding ball is provided with multiple micropore groups, each micropore group consists of 3 to 6 micropores, and the openings of the multiple micropores in each micropore group are distributed at equal intervals on the same circle;

[0008] S30. Mix the dry powder inoculant particles and the aerial seeding ball, so that the dry powder inoculant particles fill the micropores to prepare a dry powder inoculant ball for drone aerial seeding, and calculate the mass of the dry powder inoculant ball filled with the dry powder inoculant particles per unit mass.

[0009] S40. Based on the mass of the dry powder inoculant balls filled with the dry powder inoculant granules per unit mass, and combined with the mass of dry powder inoculant required for the area of ​​the aerial seeding region, calculate the total mass of the dry powder inoculant balls required for aerial seeding, and then carry out drone aerial seeding.

[0010] Furthermore, the pretreatment includes manual grinding and sieving; the dry powder inoculant granules pass through a 75-85 mesh sieve; the amount of dry powder inoculant granules added is 300-800g per acre; the dry powder inoculant granules are concentrated dry powder inoculant, and the inoculant species is dark-colored septate endophytic fungi.

[0011] Furthermore, the angle between the normal of the micropore and the tangent plane of the surface of the seeding ball at its location is 30 to 60°.

[0012] Furthermore, in each of the micropore groups, any two adjacent micropores are distributed in a figure-eight radiating pattern from the surface of the seeding ball to its center.

[0013] Furthermore, in each of the micropore groups, any two adjacent micropores are distributed in a figure-eight radiating pattern from the center of the seeding ball to its surface.

[0014] Furthermore, the diameter of the aerial seeding ball is 4.5–5.0 mm; the diameter of the micropores is 0.4–0.6 mm.

[0015] Furthermore, the micropore groups are uniformly distributed on the surface of the seeding ball; each micropore group consists of 4 micropores; the walls of the micropores are uneven; and the seeding ball is made of a biodegradable material.

[0016] Furthermore, S20 also includes: subjecting the prepared aerial seeding balls to pressure resistance treatment in an air pressure chamber, wherein the pressure increase of the air pressure chamber relative to atmospheric pressure is 10-20 kPa, in order to remove broken aerial seeding balls.

[0017] Further, mixing the dry powder microbial agent particles and the aerial seeding ball includes: performing 1 to 3 mixing and pressurization reinforcement steps, wherein mixing involves submerging the aerial seeding ball to at least half its height with the dry powder microbial agent particles, and pressurization reinforcement involves transferring the mixed dry powder microbial agent ball into an air pressure chamber and pressurizing it with compressed air, wherein the pressure increase of the air pressure chamber relative to atmospheric pressure is 10 to 20 kPa.

[0018] Furthermore, the method for aerial seeding and inoculation of dry powder microbial agents by drones also includes:

[0019] If there is rainfall before or after aerial seeding, the aerial seeding area should not be covered with soil; if there is no rainfall before or after aerial seeding, the aerial seeding area should be covered with soil.

[0020] After aerial seeding, the effectiveness of the aerial seeding is investigated to provide feedback for adjusting the aerial seeding parameters and the amount of bacteria inoculated.

[0021] The present invention has the following beneficial effects:

[0022] (1) The present invention uses the physical combination of aerial seeding balls and dry powder inoculant for filling, which is convenient and quick. After aerial seeding, the balls can fall to the ground to achieve uniform inoculation, which solves the problem of low operability of inoculating a small amount of dry powder inoculant per unit area.

[0023] (2) The specifications of the aerial seeding ball of the present invention fully take into account the needs of practical applications, including the number of hole groups, the number of holes in each hole group, and the angle between the hole normal and the tangent plane of the hole sphere surface. It has the characteristics of flexibility and strong practicality.

[0024] (3) The design of the angle between the hole normal and the tangent plane of the hole spherical surface, as well as the design of multiple orientations of the holes in the hole group, fully considers the interaction between the aerial dry powder bacterial agent ball and the air under high-speed rotation, ensuring that the dry powder bacterial agent stored in the ball is not completely destroyed by the wind force, and ensuring that the dry powder bacterial agent is carried by the aerial seeding ball and spread to the ground surface;

[0025] (4) In this invention, the physical combination of aerial seeding balls and dry powder bacterial agent is used to fill the micropores. Through multiple mixing and multiple air pressure chamber pressurization, the dry powder bacterial agent is fully filled in the micropores, so that the dry powder bacterial agent in the micropores is no longer fluffy, ensuring that the dry powder bacterial agent particles in the aerial seeding balls will not be lost due to friction and collision between aerial seeding balls and wind force during the aerial seeding process.

[0026] (5) This invention provides a dry powder microbial agent ball for drone seeding, which can be directly inoculated with dry powder microbial agent, and has strong operability and practicality; it can quickly and efficiently seed dry powder microbial agent onto various complex terrain surfaces and has broad spectrum.

[0027] (6) The aerial seeding ball material used in this invention is a green and environmentally friendly material, which has the characteristics of being green and sustainable for the ecological environment;

[0028] (7) This invention provides aerial seeding dry powder microbial agent balls for drone aerial seeding. The dry powder microbial agent balls can be mixed with other aerial seeding materials for aerial seeding, which improves the efficiency of operation. They do not produce chemical or physical reactions with each other and have no negative impact.

[0029] (8) The present invention includes an immediate survey after aerial seeding to investigate the density of the aerial seeding dry powder inoculant balls on the ground and the reserve of dry powder inoculant in the aerial seeding dry powder inoculant balls. This provides guidance for timely improvement of aerial seeding parameters, aerial seeding ball design and preparation of aerial seeding dry powder inoculant balls. It can also conduct an ecological effect survey to investigate the impact of inoculation on the ecological environment, make timely evaluations of aerial seeding parameters and inoculation status, and guide the setting of aerial seeding parameters and inoculation amount. At the same time, it also evaluates the reliability of the selection of aerial seeding ball materials, and truly implements the concept of green and sustainable ecological civilization construction. Attached Figure Description

[0030] The accompanying drawings, which form part of this application, are used to provide a further understanding of the invention. The illustrative embodiments of the invention and their descriptions are used to explain the invention and do not constitute an undue limitation of the invention. In the drawings:

[0031] Figure 1 A flowchart of the method provided by the present invention;

[0032] Figure 2 This is a schematic diagram of the "six groups of four-directional inward eight" flying seeding balls in this invention, where (a) is a front view and (b) is a front cross-sectional view;

[0033] Figure 3 This is a schematic diagram of the "six groups of four-directional outward eight" flying projectiles in this invention, where (a) is a front view and (b) is a front cross-sectional view;

[0034] Figure 4 This is a schematic diagram of the "six groups of four-directional inner eight" dry powder bacterial agent balls in this invention, where (a) is a front view and (b) is a front cross-sectional view;

[0035] Figure 5 This is a schematic diagram of the "six groups of four-directional outward eight" dry powder bacterial agent balls in this invention, where (a) is a front view and (b) is a front cross-sectional view.

[0036] The above figures include the following reference numerals:

[0037] 1. Aerial seeding ball; 2. Microporous structure; 3. Dry powder microbial agent granules. Detailed Implementation

[0038] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. The following description of at least one exemplary embodiment is merely illustrative and is in no way intended to limit the present invention or its application or use. All other embodiments obtained by those skilled in the art based on the embodiments of the present invention without inventive effort are within the scope of protection of the present invention. Unless otherwise specified, the materials, reagents, etc., used in the following embodiments are commercially available.

[0039] like Figures 1 to 5 As shown, Embodiment 1 of the present invention provides a method for aerial seeding and inoculation of dry powder microbial agents using drones.

[0040] 1. Purpose of the method design:

[0041] To address the environmental disturbance and reduced vegetation cover caused by surface cracks and collapses in underground coal mining subsidence areas, DSE dry powder inoculation was carried out.

[0042] 2. Pretreatment with dry powder microbial agent:

[0043] The pretreatment of the prepared dry powder bacterial agent mainly involves: manual grinding and sieving.

[0044] In this embodiment, the dry powder inoculant was prepared from DSE. Unfiltered DSE bacterial solution was dried at room temperature to prepare the DSE dry powder inoculant. Relevant information about DSE is as follows (specific source: Xie et al. Combined inoculation with darkseptate endophytes and arbuscular mycorrhizal fungi: synergistic or competitive growth effects on maize? BMC Plant Biol. 2021; 21:498):

[0045] Strain name: Alternaria.

[0046] Latin name: Alternaria sp.

[0047] Strain number: 001.

[0048] Preservation institution: China General Microbiological Culture Collection Center, China Microbial Culture Collection Committee.

[0049] The abbreviation for the depository is CGMCC.

[0050] Address: No. 3, Courtyard 1, Beichen West Road, Chaoyang District, Beijing.

[0051] Date of preservation: April 8, 2019.

[0052] Registered with the China National Collection Center (CGMCC) No. 17463.

[0053] Manual grinding is mainly used to pulverize the dried bacterial agent into small-particle powder.

[0054] The sieve should be 80 mesh, meaning the particle size of the dry powder inoculant should not exceed 0.180 mm.

[0055] 3. Preparation of aerial seeding balls:

[0056] The aerial seeding ball is a 5mm diameter sphere with 6 micropore groups on its surface. Each micropore group consists of 4 micropores, and the micropores in each micropore group are distributed on the same circle. The micropores are evenly spaced on the circle to which they belong. The material is biodegradable or other environmentally friendly materials.

[0057] The micropore design pattern is "six groups of four-directional inner eight", meaning that any two micropores are distributed in a "figure eight" radiating pattern from the center of the seeding ball to the surface (e.g. Figure 2 As shown), or "six groups of four-directional outward eight", that is, any two micropores are arranged in a "figure eight" radiating pattern from the surface of the seeding ball to the center of the ball (as shown). Figure 3 As shown, the micropores are 2mm deep and 0.4mm in diameter. The angle between the normal of the micropore and the tangent plane of the surface of the seeding ball at its location is 45°.

[0058] Black clay and vegetation were crushed and sieved separately and then prepared into aerial seeding balls in a 1:1 volume ratio. The prepared aerial seeding balls were subjected to pressure treatment in an air pressure chamber with an atmospheric pressure increase of 15 kPa to remove broken aerial seeding balls, which facilitated the preparation of dry powder inoculant balls and ensured the safety of the aerial seeding equipment.

[0059] 4. Preparation of dry powder inoculant balls:

[0060] To ensure better storage of the dry powder inoculant within the micropores of the aerial seeding balls, a three-stage "mixing and pressurization" process is required during the preparation of the aerial seeding balls and dry powder inoculant balls. "Mixing" involves mixing the pre-treated dry powder inoculant with the intact aerial seeding balls, ensuring the dry powder inoculant submerges at least half the height of the aerial seeding balls to facilitate its entry into the pores. "Pressure consolidation" involves transferring the mixed aerial seeding dry powder inoculant balls to an air pressure chamber, where compressed air is used for pressurization. The atmospheric pressure increment in the air pressure chamber can be selected as 15 kPa. Since the aerial seeding balls are solid spheres, the pressure generated by the compressed air compresses the dry powder inoculant deep into the micropores, preventing it from becoming fluffy. This process yields aerial seeding dry powder inoculant balls (such as...). Figure 4 and Figure 5 (As shown).

[0061] 5. Set the aerial seeding parameters and perform aerial seeding:

[0062] Calculate the amount of dry powder inoculant per unit mass of aerial seeding pellets, and then calculate the required mass of dry powder inoculant for the desired aerial seeding area. Finally, calculate the total required mass of aerial seeding pellets. The formula is as follows:

[0063]

[0064] In the formula, G is the total mass of dry powder inoculant balls required for aerial seeding, in kg; m1 is the mass of dry powder inoculant required for the sowing area, in g; and m2 is the mass of dry powder inoculant in each kg of dry powder inoculant balls, in g.

[0065] Based on the calculated mass of dry powder inoculant balls required for aerial seeding, aerial seeding parameters were designed and aerial seeding was carried out. The aerial seeding parameters were: flight height 4m, flight speed 4m / s, high leakage velocity, high rotation speed of the turntable, width 10m, and 10.3kg of dry powder inoculant balls per acre, of which 300g was dry powder inoculant.

[0066] 6. Post-aerial seeding processing:

[0067] Depending on the actual situation, aerial seeding should be carried out before or after rainfall, and no soil covering is required after aerial seeding. The dry powder inoculant balls will dissolve in rainwater after rain and infiltrate into the soil.

[0068] 7. Investigation and Evaluation of Aerial Seeding Effect:

[0069] Following aerial seeding, the effects of aerial seeding are investigated, including immediate post-seeding surveys and ecological effect surveys. Immediate surveys involve randomly selecting representative 1m×1m quadrats, with 5 quadrats per acre, and fixing these quadrats for later ecological effect surveys.

[0070] Immediate post-aerial seeding surveys primarily investigate the density of the aerially seeded dry powder inoculant balls on the ground and the reserve of dry powder inoculant within the balls. This provides guidance for timely improvements to aerial seeding parameters, ball design, and ball preparation. Ecological impact surveys aim to investigate the influence of inoculation on the ecological environment, promptly evaluate aerial seeding parameters and inoculation status, and guide the setting of aerial seeding parameters and inoculation rates. Simultaneously, the reliability of the ball material selection is evaluated, truly implementing the concept of green and sustainable ecological civilization construction.

[0071] The immediate survey results (see Table 1) show that the dry powder inoculant balls in the sample plots were intact and without any breakage. The density of the dry powder inoculant balls was 13-27 balls / m2, indicating that the dry powder in the inoculant balls was stored intact.

[0072] The ecological effect survey was conducted one year after aerial seeding. The survey showed (see Table 1) that the root infection rate of plants inoculated with dry powder mycelium (inoculated) was 7.9% higher than that of plants not inoculated with dry powder mycelium (control), the infection intensity was significantly increased by 39.8%, and the vegetation coverage was increased by 25.9%. In the field, native DSEs already exist in the soil, and these native DSEs can infect plant roots. Inoculation with dry powder mycelium increases the infection rate and has a more significant promoting effect on infection intensity. While the field environment is complex and plant growth is affected by various factors, with climate having a greater impact, inoculation with dry powder mycelium still has a certain promoting effect on plant growth.

[0073] Table 1

[0074]

[0075] Note: Different letters in the same column indicate significant differences, P<0.05.

[0076] Example 2: Effectiveness test of inoculation with dry powder bacterial agent.

[0077] 1. Purpose of the experimental design:

[0078] An experiment was conducted to test the effectiveness of inoculation with dry powder inoculants used for aerial seeding.

[0079] 2. Pretreatment with dry powder microbial agent:

[0080] The pretreatment of the prepared dry powder bacterial agent mainly involves: manual grinding and sieving.

[0081] In this example, the dry powder bacterial agent is prepared by DSE. The DSE dry powder bacterial agent is prepared by drying unfiltered DSE bacterial solution at room temperature. The DSE is the same as that in Example 1 of this invention.

[0082] Manual grinding is mainly used to pulverize the dried bacterial agent into small-particle powder.

[0083] The sieve should be 80 mesh, meaning the particle size of the dry powder inoculant should not exceed 0.180 mm.

[0084] 3. Preparation of aerial seeding balls:

[0085] The aerial seeding ball is a 5mm diameter sphere with 6 micropore groups on its surface. Each micropore group consists of 4 micropores, and the micropores in each micropore group are distributed on the same circle, with the micropores evenly spaced on the circle to which they belong. The surface is made of black clay and vegetation.

[0086] The micropore design pattern consists of six groups of four-directional inward octagons, meaning that any two micropores are distributed in a figure-eight radiating pattern from the center of the seeding ball to its surface (e.g., Figure 2 (as shown) and six groups of four-directional outward eight, that is, any two micropores are distributed in a "figure eight" shaped scattering pattern from the surface of the seeding ball to the center of the ball (as shown). Figure 3 As shown), the micropores are 2mm deep and 0.4mm in diameter. The angle between the normal of the micropore and the tangent plane of the surface of the seeding ball is 45°. The walls of the micropores are uneven, which makes it easy for dry powder bacterial agent particles to be retained.

[0087] For material selection, black clay and vegetation are crushed and sieved separately, and then prepared into aerial seeding balls in a 1:1 volume ratio. The prepared aerial seeding balls are subjected to pressure resistance treatment in an air pressure chamber with an atmospheric pressure increase of 15 kPa to remove broken aerial seeding balls, facilitate the preparation of dry powder inoculant balls, and ensure the safety of aerial seeding equipment.

[0088] 4. Preparation of dry powder inoculant balls:

[0089] To ensure better storage of the dry powder inoculant within the micropores of the aerial seeding balls, a three-stage "mixing and pressurization" process is required during the preparation of the aerial seeding balls and dry powder inoculant balls. "Mixing" involves mixing the pre-treated dry powder inoculant with the intact aerial seeding balls, ensuring the dry powder inoculant submerges at least half the height of the aerial seeding balls to facilitate its entry into the pores. "Pressure consolidation" involves transferring the mixed aerial seeding dry powder inoculant balls to an air pressure chamber, where compressed air is used for pressurization. The atmospheric pressure increment in the pressure chamber can be selected as 15 kPa. Since the aerial seeding balls are solid spheres, the pressure generated by the compressed air compresses the dry powder inoculant deep into the micropores, preventing it from becoming fluffy. This process yields the aerial seeding dry powder inoculant balls (see...). Figure 4 and Figure 5 ).

[0090] 5. Sowing and inoculation:

[0091] The plant was alfalfa, and the soil was sterilized sand (121℃, 2h). Each pot contained 3kg of alfalfa, with 30 seeds sown per pot. After emergence, seedlings were thinned to 20 plants per pot. Treatments included:

[0092] Treatment 1: Control, sterilized dry powder inoculant (121℃, 2h) + aerial seeding balls;

[0093] Treatment 2: Direct inoculation with DSE dry powder inoculant + aerial seeding balls;

[0094] Treatment 3: Inoculate with "six groups of four-position outward eight" dry powder fungal inoculant balls + cover with soil;

[0095] Treatment 4: Inoculate with "six groups of four-position outward eight" dry powder fungal balls + do not cover with soil;

[0096] Treatment 5: Inoculate with "six groups of four-position inner eight" dry powder inoculant balls + cover with soil;

[0097] Treatment 6: Inoculate with "six groups of four-position inner eight" dry powder inoculant balls + do not cover with soil.

[0098] Each treatment was replicated three times, with normal watering during the growth process. The inoculation amount per pot was 0.06g for both direct inoculation with DSE dry powder inoculant and inoculation with dry powder inoculant balls.

[0099] 6. Measurement of growth physiological status:

[0100] Plant growth and physiological status were measured after 60 days of growth, including root infection rate, leaf color value, total biomass, and net photosynthetic rate, as shown in Table 2. Statistical analysis of the results in Table 2 shows that although the root infection rate of plants inoculated with dry powder inoculant balls under soil covering was slightly higher than that under uncovered soil covering, the difference was not statistically significant. Furthermore, there was no significant difference in root infection rates between different inoculation methods; all were significantly higher than the control treatment. This indicates that both inoculation with dry powder inoculant balls and direct inoculation with DSE dry powder inoculant can effectively infect plant roots and form a good symbiotic relationship with the plants. The fact that DSE can still infect plant roots under uncovered soil covering conditions is because the dry powder inoculant in the aerially seeded balls infiltrates into the soil with the water after watering, contacting the roots and thus exerting its effect. Regardless of the inoculation method, the leaf color value of the inoculated plants was significantly increased by 10.6%–15.2% compared to the control, the total biomass was significantly increased by 37.0%–45.5%, and the net photosynthetic rate was increased by 18.8%–42.3%. This demonstrates that the inoculation method of adding dry powder inoculant balls according to the present invention can effectively infect the roots of alfalfa plants, improve leaf color value, increase net photosynthetic rate and total biomass, and promote plant growth.

[0101] Table 2

[0102]

[0103] The experimental design for DSE dry powder inoculant inoculation in coal mining subsidence areas based on the method of the present invention was conducted considering the low efficiency and low operability of manual inoculation of dry powder inoculants, and has the following beneficial effects:

[0104] (1) This example method is green and sustainable in terms of the ecological environment, and the aerial seeding ball material used is a green and environmentally friendly material;

[0105] (2) This example method uses the physical combination of aerial seeding balls and dry powder inoculant to fill the micropores. Through multiple "mixing and pressurization reinforcement", the dry powder inoculant is fully filled in the micropores. This method is convenient and quick, and solves the problem of low operability of inoculating a small amount of dry powder inoculant per unit area.

[0106] (3) The method in this example can quickly and efficiently spread dry powder microbial agent to various complex terrain surfaces by using drones to spread dry powder microbial agent balls.

[0107] (4) This example method fully considers the conditions under which the aerial seeding dry powder inoculant balls can exert their effects on the ground. It selects whether to carry out soil covering treatment based on whether there is rainfall before and after aerial seeding. It fully considers the actual situation, minimizes manual input as much as possible, and ensures that the inoculation is standardized and effective.

[0108] (5) The specifications of the flying seed ball in this example fully consider the needs of practical applications, taking into account the number of directions of the hole group, the number of directions of the holes in each hole group, and the angle relationship between the hole normal and the tangent plane of the hole sphere surface. It has the characteristics of flexibility and strong practicality.

[0109] (6) In this example, the effects of inoculating with dry powder inoculant balls and directly inoculating with DSE dry powder inoculant on plant root infection are similar under both soil-covered and non-soil-covered conditions. Inoculating with dry powder inoculant balls can achieve similar effects to directly inoculating with DSE dry powder inoculant.

[0110] (7) This example method considers the monitoring of the immediate effects of aerial seeding and the ecological restoration effects of aerial seeding, providing valuable suggestions for guiding the setup of aerial seeding inoculation, the selection of aerial seeding ball materials, and the restoration of the ecological environment. It is evident that the method for inoculating with DSE dry powder inoculant designed in this example can directly guide the inoculation of dry powder inoculant, demonstrating strong operability and practicality.

[0111] The above description is merely a preferred embodiment of the present invention and is not intended to limit the invention. Various modifications and variations can be made to the present invention by those skilled in the art. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the scope of protection of the present invention.

[0112] It should be noted that the terminology used herein is for the purpose of describing particular embodiments only and is not intended to limit the exemplary embodiments according to this application. As used herein, the singular form is intended to include the plural form as well, unless the context clearly indicates otherwise. Furthermore, it should be understood that when the terms "comprising" and / or "including" are used in this specification, they indicate the presence of features, steps, operations, devices, components, and / or combinations thereof.

[0113] Unless otherwise specifically stated, the relative arrangement, numerical expressions, and values ​​of the components and steps described in these embodiments do not limit the scope of the invention. It should also be understood that, for ease of description, the dimensions of the various parts shown in the drawings are not drawn to actual scale. Techniques, methods, and devices known to those skilled in the art may not be discussed in detail, but where appropriate, such techniques, methods, and devices should be considered part of the specification. In all examples shown and discussed herein, any specific values ​​should be interpreted as exemplary only and not as limitations. Therefore, other examples of exemplary embodiments may have different values. It should be noted that similar reference numerals and letters in the following drawings denote similar items; therefore, once an item is defined in one drawing, it need not be further discussed in subsequent drawings.

[0114] In the description of this invention, it should be understood that the orientation or positional relationship indicated by directional terms such as "front, back, up, down, left, right", "horizontal, vertical, horizontal" and "top, bottom" is generally based on the orientation or positional relationship shown in the accompanying drawings, and is only for the convenience of describing this invention and simplifying the description. Unless otherwise stated, these directional terms do not indicate or imply that the device or element referred to must have a specific orientation or be constructed and operated in a specific orientation, and therefore should not be construed as a limitation on the scope of protection of this invention; the directional terms "inner" and "outer" refer to the inner and outer contours relative to the outline of each component itself.

[0115] For ease of description, spatial relative terms such as "above," "on top of," "on the upper surface of," "above," etc., are used herein to describe the spatial positional relationship of a device or feature as shown in the figures to other devices or features. It should be understood that spatial relative terms are intended to encompass different orientations in use or operation beyond the orientation of the device as described in the figures. For example, if the device in the figures were inverted, a device described as "above" or "on top of" other devices or structures would subsequently be positioned as "below" or "under" other devices or structures. Thus, the exemplary term "above" can include both "above" and "below." The device may also be positioned in other different ways (rotated 90 degrees or in other orientations), and the spatial relative descriptions used herein will be interpreted accordingly.

[0116] Furthermore, it should be noted that the use of terms such as "first" and "second" to define components is merely for the purpose of distinguishing the corresponding components. Unless otherwise stated, the above terms have no special meaning and therefore should not be construed as limiting the scope of protection of this invention.

Claims

1. A method for aerial seeding and inoculation of dry powder microbial agents using unmanned aerial vehicles, characterized in that, Includes the following steps: S10. The prepared dry powder bacterial agent is pretreated to obtain dry powder bacterial agent granules; the pretreatment includes manual grinding and sieving. The dry powder inoculant is passed through a 75-85 mesh sieve; the dosage of the dry powder inoculant granules is 300-800g per acre; the dry powder inoculant is a concentrated dry powder inoculant, and the inoculant species is dark-colored septate endophytic fungi; S20. Preparation of aerial seeding ball: The surface of the aerial seeding ball is provided with multiple micropore groups, each micropore group consists of 4 micropores, and the openings of the 4 micropores in each micropore group are equally spaced on the same circle; The prepared aerial seeding balls are subjected to pressure resistance treatment in an air pressure chamber, where the pressure increase of the air pressure chamber relative to atmospheric pressure is 10~20 kPa, in order to remove broken aerial seeding balls. S30. The dry powder inoculant particles are filled into the micropores to prepare a dry powder inoculant ball for drone aerial seeding, and the mass of the dry powder inoculant ball per unit mass of the dry powder inoculant particles is calculated. Filling the micropores with the dry powder microbial agent particles includes: performing 1 to 3 mixing and pressurization reinforcement steps, wherein mixing involves submerging the aerial seeding ball at least half its height with the dry powder microbial agent particles, and pressurization reinforcement involves transferring the mixture obtained from mixing into an air pressure chamber and pressurizing and reinforcing it by compressed air, wherein the pressure increment of the air pressure chamber relative to atmospheric pressure is 10 to 20 kPa. S40. Based on the mass of the dry powder inoculant balls filled with the dry powder inoculant granules per unit mass, and combined with the mass of dry powder inoculant required for the area of ​​the aerial seeding region, calculate the total mass of the dry powder inoculant balls required for aerial seeding, and then carry out drone aerial seeding. The angle between the normal of the micropore and the tangent plane of the surface of the seeding ball at its location is 30-60°; the diameter of the seeding ball is 4.5-5.0 mm; the diameter of the micropore is 0.4-0.6 mm; the micropore group is uniformly distributed on the surface of the seeding ball; the walls of the micropore are uneven; the seeding ball is made of biodegradable material.

2. The method for inoculating dry powder microbial agent by drone seeding according to claim 1, characterized in that, In each group of micropores, any two adjacent micropores are distributed in a figure-eight radiating pattern from the surface of the seeding ball to its center.

3. The method for inoculating dry powder microbial agent by drone aerial seeding according to claim 1, characterized in that, In each group of micropores, any two adjacent micropores are distributed in a figure-eight radiating pattern from the center of the seeding ball to its surface.

4. The method for inoculating dry powder microbial agent by drone aerial seeding according to claim 1, characterized in that, The method for aerial seeding and inoculation of dry powder microbial agents by drones also includes: If there is rainfall before or after aerial seeding, the aerial seeding area should not be covered with soil; if there is no rainfall before or after aerial seeding, the aerial seeding area should be covered with soil. After aerial seeding, the effectiveness of the aerial seeding is investigated to provide feedback for adjusting the aerial seeding parameters and the amount of bacteria inoculated.

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