Human body rectum mucosa potential measuring instrument and measuring method

Abstract

The invention relates to the technical field of mucous membrane potential measurement, and discloses a human body rectum mucous membrane potential measuring instrument which comprises a detection electrode and a high input impedance electrometer and further comprises a reference electrode, the rear end of the reference electrode is connected with the low input end of the high input impedance electrometer through a wire, and the front end of the reference electrode penetrates into perianal subcutaneous skin. The reference electrode injects sterile normal saline to eliminate polarization potential so as to obtain stable reference potential; the rear end of the exploring electrode is connected to the high input end of the high input impedance electrometer through a wire, and the front end of the exploring electrode is led into the rectum from the anus through the sigmoid colonoscope to make contact with the rectum mucosa so as to obtain measuring potential. The reference electrode comprises a reference silver wire, an injector and a hollow needle head detachably installed at the front end of the injector, and the hollow needle head comprises a needle tip, a needle stem and a pintle. According to the invention, the measurement accuracy of the human body rectum mucous membrane potential can be effectively improved, so that reliable and accurate data support is provided for diagnosis and treatment of related diseases of rectum.

Description

Technical Field

[0001] The invention relates to the technical field of mucosal potential measurement, and in particular to a human rectal mucosal potential measuring instrument and a measuring method. Background Art

[0002] The rectum is an important part of the human digestive system, and the normality of its physiological function is crucial to human health. Changes in the rectal mucosal potential can reflect a variety of physiological states, such as the electrical activity of the rectal muscles and the function of the intestinal nerves. At present, the basic measurement scheme for the rectal mucosal potential is still based on the indirect measurement method in 1975, which uses the human skin potential as the reference potential, that is, calculating the rectal mucosal potential through multiple measurements. The approximate estimation used in the calculation of this method will reduce the reliability of the final result, and it is difficult to obtain accurate rectal mucosal potential data in clinical practice, thus affecting the diagnosis and treatment of rectal-related diseases.

[0003] There are also some studies related to rectal mucosal potential in current technology, which mainly focus on the structural modification of rectal electrode probes. For example, the Chinese utility model patent with application number 202320469260.8, named a rectal electrode probe for biofeedback therapy instrument, modifies the structure of the electrode probe head so that it can be automatically guided during placement, increases the clamping force of the rectal inner wall on the electrode probe, and makes the electrode probe cooperate with the inner wall of the anus and rectum when inserted into the anus and rectum, reducing the risk of accidental withdrawal of the electrode probe from the anus and rectum; by providing a heating component and a temperature sensor, the electrode probe can be preheated before use, so that when the electrode probe structure is inserted into the rectum, the patient will not have a bad experience due to the cold surface of the probe body. However, it only modified the structure of the rectal electrode probe, and did not involve the improvement of the reference potential and measurement accuracy.

[0004] Another example is the Chinese invention patent with application number 202211661462.9, entitled A rectal electrode and a rectal biofeedback and contraction and relaxation system, and a transrectal probe, which includes an electrode body and a rectal electrode rectal biofeedback and contraction and relaxation system. The electrode body realizes biofeedback (electromyographic signal) and electrical stimulation of the puborectalis muscle through the middle electrode sheet, and realizes biofeedback (electromyographic signal) and electrical stimulation of the anal sphincter through the rear electrode sheet. Each electrode can independently adjust its own frequency and current intensity to achieve the purpose of coordinated movement training of the puborectalis muscle and anal sphincter and to achieve precision training. In essence, it is still only a modification of the rectal electrode, and the human skin potential is used as the reference potential, so there is no change in the measurement method.

[0005] Another example is the Chinese invention patent with application number 201910690430.3, entitled Transrectal Probe, Electrical Impedance Tomography System and Method. The signal-to-noise ratio of the prostate electrical impedance signal collected by the patent is relatively high, which can realize the collection of prostate electrical impedance signals, enhance the strength of prostate electrical impedance signals and reduce the influence of other interference signals. In clinical applications, the transrectal probe collects prostate electrical impedance signals with a high signal-to-noise ratio due to its proximity to the prostate. Then, the electrical impedance tomography image after image reconstruction can detect the distribution changes of electrical impedance in the prostate tissue as early as possible. However, this patent is mainly an improvement of the imaging system, and is used to realize electrical impedance tomography of the prostate. There is no modification to the method of measuring the rectal mucosal potential, and there is no improvement in the measurement accuracy. Summary of the invention

[0006] In order to overcome the shortcomings of the prior art, the purpose of the present invention is to provide a human rectal mucosal potential measuring instrument and a measuring method, which can effectively improve the measurement accuracy of human rectal mucosal potential, thereby providing reliable and accurate data support for the diagnosis and treatment of rectal related diseases.

[0007] The present invention is implemented by the following technical scheme: a human rectal mucosal potential measuring instrument, comprising a detection electrode and a high-input impedance electrometer, and also comprising a reference electrode, wherein the rear end of the reference electrode is connected to the low input end of the high-input impedance electrometer through a wire, the front end of the reference electrode is inserted into the perianal subcutaneous tissue, and sterile physiological saline is injected into the reference electrode to eliminate the polarization potential for obtaining a stable reference potential; the rear end of the detection electrode is connected to the high input end of the high-input impedance electrometer through a wire, and the front end of the detection electrode is introduced into the rectum from the anus by a sigmoidoscope to contact the rectal mucosa for obtaining the measurement potential.

[0008] Furthermore, the reference electrode includes a reference silver wire, a syringe and a hollow needle detachably mounted on the front end of the syringe, the hollow needle includes a needle tip, a needle stem and a needle plug, the needle tip is integrally formed and arranged at the front end of the needle stem, the needle tip is inclined at a certain angle, the needle plug is connected to the rear end of the needle stem, the reference silver wire is arranged in the center of the needle stem, and a solution channel for the solution to pass through is left between the outer ring of the reference silver wire and the inner wall of the needle stem; the reference silver wire is introduced into the subcutaneous tissue around the anus through the hollow needle, and sterile saline is injected into the subcutaneous tissue around the anus through the hollow needle using a syringe, and the syringe is removed after the injection is completed, leaving the hollow needle and the reference silver wire in the skin.

[0009] Furthermore, the detection electrode includes an insulating rubber probe and a detection silver wire, the insulating rubber probe is wrapped around the outside of the detection silver wire, and the detection electrode is used in conjunction with a sigmoidoscope probe, so that it is introduced into the rectum by the sigmoidoscope and the rectal mucosal potential is detected under direct vision of the lens.

[0010] Furthermore, the front part of the insulating rubber probe and the detection silver wire are both curved, so that the detection electrode can stably contact the rectal mucosa, and the entire probe part is passivated to prevent scratching the skin.

[0011] Furthermore, the diameter of the detection electrode is 4-6 mm.

[0012] Furthermore, the hollow needle is made of insulating material, the diameter of the needle stem is 0.8-1.2 mm, and the needle tip is inclined at 45°.

[0013] Furthermore, the outer surfaces of the reference silver wire and the detection silver wire are coated with an AgCl layer.

[0014] Furthermore, a mounting hole is provided on the needle bolt, and the rear end of the reference silver wire passes through the mounting hole. The mounting hole is sealed with gum to prevent leakage when injecting sterile saline.

[0015] A method for measuring the potential of a human rectal mucosa, using the human rectal mucosa potential measuring instrument, comprises the following steps:

[0016] Step 1: Connect the rear end of the reference electrode to the low input terminal of the high input impedance electrometer through a wire, and connect the rear end of the detection electrode to the high input terminal of the high input impedance electrometer through a wire;

[0017] Step 2: Place the reference electrode and the detection electrode together in an isotonic NaCl solution, select the 0-100mV range, and adjust the reading of the high input impedance electrometer to below 1mV to eliminate the influence of the asymmetric voltage between the two electrodes on the measurement results;

[0018] Step 3: Install the hollow needle on the front nipple of the syringe barrel, add sterile saline into the syringe barrel, insert the reference electrode into the subcutaneous tissue 2-3 cm around the anus, and inject 0.4-0.6 ml of sterile saline;

[0019] Step 4: After the injection is completed, separate and withdraw the syringe, leaving only the insulated hollow needle and the reference silver wire inside on the perianal skin;

[0020] Step 5: Soak the detection electrode in sterile saline, and then introduce it into the rectum 5-10 cm inside the anus through the sigmoidoscope, and contact the rectal mucosa under direct vision of the sigmoidoscope. After the high input impedance electrometer reading is stable, read and record the high input impedance electrometer reading;

[0021] Step 6: After the detection electrode leaves the mucosa for a short time, it contacts the same position of the mucosa again. After the reading of the high input impedance electrometer stabilizes, read and record the reading of the electrometer. Repeat this step and read 3-5 sets of reasonable data at each point.

[0022] Step 7: Take out the reference electrode and detection electrode, and clean and disinfect the electrodes and skin.

[0023] Compared with the prior art, the present invention has the following beneficial effects:

[0024] 1. Compared with the rectal mucosal potential measurement technology using a reference potential in a traditional potential measuring instrument, the human rectal mucosal potential measuring instrument and the measuring method of the present invention directly use the subcutaneous potential of the perianal skin as the reference potential and remove the skin polarization potential before measurement to ensure the accuracy of the measurement results. At the same time, the probe contact is more sufficient, which can effectively improve the accuracy of the measurement data and improve the measurement accuracy of the traditional method from 1mV to 0.1mV, thereby providing reliable and accurate data support for the diagnosis and treatment of rectal related diseases and improving the accuracy of disease diagnosis.

[0025] 2. The human rectal mucosal potential measuring instrument of the present invention, through the special structural design of the reference electrode and the detection electrode, enables the electrodes to better contact with human tissues, thereby improving the accuracy and stability of the measurement.

[0026] 3. The human rectal mucosal potential measuring instrument and measuring method of the present invention have high applicability, and can also be applied to traditional indirect measurement methods, using skin potential as a reference potential to indirectly calculate the potential difference between the inside and outside of the rectal mucosa. BRIEF DESCRIPTION OF THE DRAWINGS

[0027] Figure 1 It is a connection schematic diagram of the human rectal mucosal potential measuring instrument of the present invention when it is used;

[0028] Figure 2 It is a schematic diagram of the connection structure between the hollow needle and the syringe in the present invention;

[0029] Figure 3 It is a schematic diagram of the structure of the reference silver wire in the needle stem of the present invention;

[0030] Figure 4 It is a schematic diagram of the structure of the reference silver wire in the hollow needle in the present invention;

[0031] Figure 5 It is a schematic diagram of the structure of the detection electrode in the present invention;

[0032] Figure 6 It is a schematic diagram of the operation of the present invention when used to measure the potential of the rectal mucosa of a human body.

[0033] In the figure: reference electrode-1; detection electrode-2; high input impedance electrometer-3; hollow needle-11; needle tip-111; needle stem-112; needle plug-113; mounting hole-114; syringe-12; reference silver wire-13; solution channel-14; syringe-121; nipple-122; piston-123; piston handle-124; insulating rubber probe-21; detection silver wire-22. DETAILED DESCRIPTION

[0034] The following describes the embodiments of the present invention by specific examples, and those skilled in the art can easily understand other advantages and effects of the present invention from the contents disclosed in this specification. The present invention can also be implemented or applied through other different specific embodiments, and the details in this specification can also be modified or changed in various ways based on different viewpoints and applications without departing from the spirit of the present invention. It should be noted that the following embodiments and features in the embodiments can be combined with each other without conflict.

[0035] The purpose of the present invention is to provide a human rectal mucosal potential measuring instrument and a measuring method in view of the defects of the prior art.

[0036] Embodiment 1

[0037] This embodiment provides a human rectal mucosal potential measuring instrument, referring to Figure 1 As shown, it includes a reference electrode 1, a detection electrode 2 and a high input impedance electrometer 3, wherein the rear end of the reference electrode 1 is connected to the low input end of the high input impedance electrometer 3 through a wire, the front end of the reference electrode 1 is inserted into the perianal subcutaneous tissue, and the reference electrode 1 is injected with sterile saline to eliminate the polarization potential for obtaining a stable reference potential. The rear end of the detection electrode 2 is connected to the high input end of the high input impedance electrometer 3 through a wire, and the front end of the detection electrode 2 is introduced from the anus into the rectum by a sigmoidoscope to contact the rectal mucosa for obtaining the measurement potential.

[0038] The high input impedance electrometer 3 in this embodiment uses a high-precision, high input impedance electrometer in the prior art, and its voltage resolution is on the order of 0.1 mV. Since the potential of the human rectal mucosa is generally -40±5 mV, the high input impedance electrometer 3 can better meet the needs of measuring the potential of the human rectal mucosa. Through the human rectal mucosa potential measuring instrument, the internal and external potential difference of the rectal mucosa can be directly read on the high input impedance electrometer 3, which is convenient to use.

[0039] Reference Figure 2-Figure 4 As shown, the reference electrode 1 includes a reference silver wire 13 , a syringe 12 , and a hollow needle 11 detachably mounted on the front end of the syringe 12 .

[0040] The hollow needle 11 includes a needle tip 111, a needle stem 112 and a needle plug 113. The needle tip 111 is integrally formed and arranged at the front end of the needle stem 112. The needle tip 111 is inclined at a certain angle. In this embodiment, the needle tip 111 is 45 degrees, and the needle plug 113 is connected to the rear end of the needle stem 112. The hollow needle 11 is made of insulating material, and the diameter of its needle stem 112 is 0.8~1.2mm. In this embodiment, the diameter of the needle stem 112 is 1mm. A mounting hole 114 is provided on the needle plug 113, and the rear end of the reference silver wire 13 passes through the mounting hole 114. The mounting hole 114 is sealed with gum to prevent leakage when injecting sterile saline.

[0041] The syringe 12 includes a syringe 121, a nipple 122, a piston 123 and a piston handle 124. The nipple 122 is integrally formed at the front end of the syringe 121, the piston 123 is arranged in the syringe 121, and the piston handle 124 is fixed at the rear end of the piston 123. A certain amount of solution can be injected through the syringe 12.

[0042] The reference silver wire 13 is arranged in the center of the needle stem 112 , and a solution channel 14 for the solution to pass through is reserved between the outer circle of the reference silver wire 13 and the inner wall of the needle stem 112 .

[0043] The reference silver wire 13 is introduced into the subcutaneous tissue around the anus through the hollow needle 11, and sterile saline is injected into the subcutaneous tissue around the anus through the hollow needle 11 using the syringe 12. After the injection is completed, the syringe 12 is removed, leaving the hollow needle 11 and the reference silver wire 13 in the skin.

[0044] Embodiment 2

[0045] This embodiment provides a human rectal mucosal potential measuring instrument, referring to Figure 5 As shown, the detection electrode 2 includes an insulating rubber probe 21 and a detection silver wire 22. The insulating rubber probe 21 is wrapped around the outside of the detection silver wire 22. It is made of medical insulating rubber material. The detection electrode 2 is used in conjunction with the sigmoidoscope probe, so that it is introduced into the rectum by the sigmoidoscope and the rectal mucosal potential is detected under the direct view of the lens, which is convenient for accurately placing the detection electrode 2 on the rectal mucosa, further improving the accuracy of the measurement. Since the surface of the rectum is not smooth, in this embodiment, the front of the insulating rubber probe 21 and the detection silver wire 22 are designed to be curved, so that the detection electrode 2 can stably contact the rectal mucosa, and the entire probe part is passivated to prevent scratching the skin. The diameter of the detection electrode 2 is 4-6mm, and the diameter of the detection electrode 2 in this embodiment is 5mm. The outer surfaces of the reference silver wire 13 and the detection silver wire 22 are coated with an AgCl layer to enable better conduction. Through the special structural design of the reference electrode 1 and the detection electrode 2, the electrodes can better contact with human tissues, improving the accuracy and stability of the measurement.

[0046] Embodiment 3

[0047] A method for measuring the potential of human rectal mucosa, using the above-mentioned human rectal mucosa potential measuring instrument, referring to Figure 6 As shown, the following steps are included:

[0048] Step 1: Connect the rear end of the reference silver wire 13 in the reference electrode 1 to the low input end of the high input impedance electrometer 3 through a wire, and connect the rear end of the detection silver wire 22 in the detection electrode 2 to the high input end of the high input impedance electrometer 3 through a wire.

[0049] Step 2: Place the reference electrode 1 and the detection electrode 2 together in an isotonic NaCl solution, select a 0-100mV range, and adjust the reading of the high input impedance electrometer 3 to below 1mV to eliminate the influence of the asymmetric voltage between the two electrodes on the measurement result.

[0050] Step 3: Install the hollow needle 11 on the front end nipple of the syringe 12, add sterile saline into the syringe 12, insert the reference electrode 1 into the subcutaneous tissue 2-3 cm around the anus, and inject 0.4-0.6 ml of sterile saline.

[0051] Step 4: After the injection is completed, the syringe 12 is separated and withdrawn, leaving only the insulated hollow needle 11 and the reference silver wire 13 inside it on the perianal skin.

[0052] Step 5: Soak the detection electrode 2 in sterile saline, and then introduce it into the rectum 5-10 cm inside the anus through a sigmoidoscope, and contact it with the rectal mucosa under direct vision of the sigmoidoscope. After the reading of the high input impedance electrometer 3 stabilizes, read and record the reading of the high input impedance electrometer 3.

[0053] Step 6: After the detection electrode 2 leaves the mucosa for a short time, it contacts the same position of the mucosa again. After the reading of the high input impedance electrometer 3 stabilizes, read and record the reading of the electrometer; repeat this step and read 3-5 sets of reasonable data at each point.

[0054] Step 7: Take out the reference electrode 1 and the detection electrode 2, and clean and disinfect the electrodes and skin.

[0055] Operators must wear goggles, masks and medical sterile gloves throughout the operation and disinfect all laboratory equipment before wearing them.

[0056] The present invention discloses a human rectal mucosal potential measuring instrument and a measuring method. Compared with the rectal mucosal potential measuring method in which the traditional potential measuring instrument uses the skin potential as the reference potential, the present invention directly uses the subcutaneous potential of the perianal skin as the reference potential, and injects sterile saline to form blisters before measurement to remove the skin polarization potential to ensure the accuracy of the measurement result, while making the probe contact more sufficient, which can effectively improve the accuracy of the measurement data, and improve the measurement accuracy of the traditional method from 1mV level to 0.1mV level, thereby providing reliable and accurate data support for the diagnosis and treatment of rectal related diseases.

[0057] At the same time, the present invention has high applicability and can also be applied to traditional indirect measurement methods. It uses skin potential as a reference potential and indirectly calculates the potential difference between the inside and outside of the rectal mucosa by measuring the perianal skin potential, the body surface skin potential and the outer surface potential of the rectal mucosa.

[0058] In addition, it should be understood that although the present specification is described according to implementation modes, not every implementation mode contains only one independent technical solution. This description of the specification is only for the sake of clarity. Those skilled in the art should regard the specification as a whole. The technical solutions in each embodiment may also be appropriately combined to form other implementation modes that can be understood by those skilled in the art.

Claims

1. A human rectal mucosal potential measuring instrument, comprising a detection electrode (2) and a high input impedance electrometer (3), characterized in that: Also included is a reference electrode (1), The rear end of the reference electrode (1) is connected to the low input end of the high input impedance electrometer (3) through a wire, the front end of the reference electrode (1) is inserted into the perianal subcutaneous tissue, and sterile physiological saline is injected into the reference electrode (1) to eliminate the polarization potential and obtain a stable reference potential; The rear end of the detection electrode (2) is connected to the high input end of the high input impedance electrometer (3) through a wire, and the front end of the detection electrode (2) is introduced from the anus into the rectum by a sigmoidoscope to contact the rectal mucosa for obtaining the measurement potential.

2. The human rectal mucosal potential measuring instrument according to claim 1, characterized in that: The reference electrode (1) comprises a reference silver wire (13), a syringe (12) and a hollow needle (11) detachably mounted on the front end of the syringe (12); the hollow needle (11) comprises a needle tip (111), a needle stem (112) and a needle plug (113); the needle tip (111) is integrally formed and arranged at the front end of the needle stem (112); the needle tip (111) is inclined at a certain angle; the needle plug (113) is connected to the rear end of the needle stem (112); The reference silver wire (13) is arranged at the center of the needle stem (112), and a solution channel (14) for the solution to pass through is reserved between the outer ring of the reference silver wire (13) and the inner wall of the needle stem (112); the reference silver wire (13) is introduced into the subcutaneous tissue around the anus through the hollow needle (11), and sterile saline is injected into the subcutaneous tissue around the anus through the hollow needle (11) using a syringe (12); after the injection is completed, the syringe (12) is removed, and the hollow needle (11) and the reference silver wire (13) are left in the skin.

3. The human rectal mucosal potential measuring instrument according to claim 2, characterized in that: The detection electrode (2) comprises an insulating rubber probe (21) and a detection silver wire (22), wherein the insulating rubber probe (21) is wrapped around the outside of the detection silver wire (22). The detection electrode (2) is used in conjunction with a sigmoidoscope probe so that the sigmoidoscope is introduced into the rectum and the rectal mucosal potential is detected under direct vision of the camera.

4. The human rectal mucosal potential measuring instrument according to claim 3, characterized in that: The front parts of the insulating rubber probe (21) and the detection silver wire (22) are both curved, so that the detection electrode (2) can stably contact the rectal mucosa, and the entire probe part is passivated to prevent scratching the skin.

5. The human rectal mucosal potential measuring instrument according to claim 3, characterized in that: The diameter of the detection electrode (2) is 4-6 mm.

6. The human rectal mucosal potential measuring instrument according to claim 2, characterized in that: The hollow needle (11) is made of insulating material, the diameter of the needle stem (112) is 0.8-1.2 mm, and the needle tip (111) is inclined at 45°.

7. The human rectal mucosal potential measuring instrument according to claim 4, characterized in that: The outer surfaces of the reference silver wire (13) and the detection silver wire (22) are both provided with an AgCl layer.

8. The human rectal mucosal potential measuring instrument according to claim 4, characterized in that: The needle plug (113) is provided with a mounting hole (114), and the rear end of the reference silver wire (13) passes through the mounting hole (114). The mounting hole (114) is sealed with gum to prevent leakage when injecting sterile saline.

9. A method for measuring the potential of a human rectal mucosa, using the human rectal mucosal potential measuring instrument according to any one of claims 1 to 8, characterized in that: The following steps are involved: Step 1: Connect the rear end of the reference electrode (1) to the low input end of the high input impedance electrometer (3) through a wire, and connect the rear end of the detection electrode (2) to the high input end of the high input impedance electrometer (3) through a wire; Step 2: Place the reference electrode (1) and the detection electrode (2) together in an isotonic NaCl solution, select a 0-100 mV range, and adjust the reading of the high input impedance electrometer (3) to below 1 mV to eliminate the influence of the asymmetric voltage between the two electrodes on the measurement result; Step 3: Install the hollow needle (11) on the front end nipple of the syringe (12), add sterile saline into the syringe (12), insert the reference electrode (1) into the subcutaneous tissue 2-3 cm around the anus, and inject 0.4-0.6 ml of sterile saline; Step 4: After the injection is completed, the syringe (12) is separated and withdrawn, leaving only the insulated hollow needle (11) and the reference silver wire (13) inside it on the perianal skin; Step 5: Soak the detection electrode (2) in sterile physiological saline, and then introduce it into the rectum 5-10 cm inside the anus through a sigmoidoscope, and contact the rectal mucosa under direct vision of the sigmoidoscope. After the reading of the high input impedance electrometer (3) stabilizes, read and record the reading of the high input impedance electrometer (3); Step 6: After the detection electrode (2) leaves the mucosa for a short time, it contacts the same position of the mucosa again. After the reading of the high input impedance electrometer (3) stabilizes, read and record the reading of the electrometer. Repeat this step and read 3-5 sets of reasonable data at each point. Step 7: Take out the reference electrode (1) and the detection electrode (2), and clean and disinfect the electrodes and skin.

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