Preparation method of special pre-medicated bath disinfectant for dairy farm

Abstract

The preparation method of the special pre-medicated bath disinfectant for the dairy farm comprises the following steps: S01, adding zinc gluconate into micro-nano zinc, heating to 20-50 DEG C, stirring for 2-4 hours, adding sodium hyaluronate, continuously stirring for 1-2 hours, and cooling to obtain a precursor solution; s02, adding potassium iodate, glycerol, vitamin E and deionized water into the precursor solution, stirring for 1-4 hours at the temperature of 45-85 DEG C, and cooling to obtain a pre-medicated bath disinfectant; the micro-nano zinc pre-medicated bath disinfectant for the dairy cow, prepared by the invention, is actually non-toxic, can be used for quickly sterilizing, is non-irritant to mucous membranes, and is prepared for cleaning treatment before milking.

Description

Technical Field

[0001] The invention belongs to the technical field of animal husbandry, and in particular relates to a method for preparing a special pre-medication bath disinfectant for a dairy farm. Background Art

[0002] Cow mastitis is a common disease in dairy cow farming, which has a great impact on the health and milk production of dairy cows. Medicated bathing is one of the important measures for the prevention and treatment of cow mastitis, and plays a pivotal role in the prevention of cow mastitis.

[0003] Medicated bath refers to the use of a certain concentration of liquid medicine to clean, disinfect and treat the nipples and milk ducts of dairy cows to prevent the occurrence and spread of mastitis in dairy cows. Medicated bath can kill pathogens in the nipple area, reduce the chance of bacterial contamination and cross infection, soften the skin, promote metabolism, enhance immunity, and relieve the discomfort of dairy cows. Medicated bath can play a dual role of prevention and treatment, especially suitable for the prevention and treatment of early inflammation and mild inflammation.

[0004] Cow medicated dip is an important tool for cow health care and disease prevention, among which povidone-iodine solution is the most commonly used drug. This drug has a broad-spectrum bactericidal and disinfecting effect, and its bactericidal principle is mainly based on povidone-iodine. However, iodine is highly volatile and can sublimate at room temperature, so the bactericidal effect is not persistent.

[0005] The present invention fills this gap. The small particle size of micro-nano zinc leads to enhanced surface reactivity and activity of the particles, and the existence range of many microorganisms ranges from hundreds of nanometers to tens of micrometers. Nanoscale metal particles have a large specific surface area and accumulate higher potential energy. Generally speaking, there are nanoparticle effects, specific surface area effects, quantum tunneling effects, and reactive oxygen species (ROS) effects, etc. Various effects work synergistically to form better antibacterial activity.

[0006] Micro-nano zinc nanoparticles and released zinc ions react with -NH2, -COOH, -SH, etc. in bacteria, thereby destroying the structural composition of the cells, preventing their reproduction, and achieving the effect of killing bacteria and molds; the released zinc ions have a significant effect on active transport inhibition, amino acid metabolism and enzyme systems.

[0007] Micro-nano zinc is released from dead bacteria and starts a new round of sterilization, which repeats over and over again. Summary of the invention

[0008] In order to solve the above problems, the present invention discloses a method for preparing a special pre-medication bath disinfectant for dairy farms.

[0009] To achieve the above object, the technical solution of the present invention is as follows:

[0010] The present invention provides a method for preparing a pre-drug bath disinfectant solution for dairy farms, comprising the following steps:

[0011] Add zinc gluconate to S01 micro-nano zinc, heat to 20°C-50°C, stir for 2h-4h, add sodium hyaluronate, continue stirring for 1h-2h, cool, and prepare a precursor solution;

[0012] Potassium iodate, glycerol, vitamin E and deionized water are added to the S02 precursor solution, stirred for 1 h to 4 h at a temperature of 45° C. to 85° C., and then cooled to obtain a pre-drug bath disinfectant solution.

[0013] Furthermore, in step S01, the mass ratio of micro-nano zinc to zinc gluconate is 200:1 to 50:1.

[0014] Furthermore, in step S01, the mass ratio of micro-nano zinc to sodium hyaluronate is 20000:1 to 1000:1.

[0015] Furthermore, in step S02, the mass ratio of the precursor solution to potassium iodate is 100:1 to 10:1.

[0016] Furthermore, in step S02, the mass ratio of potassium iodate, glycerol, vitamin E, and deionized water is (1-10): (1-15): (0.1-2): (73-97.9).

[0017] Beneficial effects of the present invention:

[0018] Potassium iodate, which can quickly kill bacteria, is added to the present invention, and micro-nano zinc and potassium iodate are fully and evenly dispersed in the product through other ingredients in the present invention, such as sodium hyaluronate, glycerol and vitamin E, so that the product has both rapid sterilization and long-lasting antibacterial effects. Using a potassium iodate solution containing 60 mg / L of available iodine for 1 to 2 minutes can kill bacteria; using a potassium iodide solution containing 50 mg / L of available iodine for 10 minutes can kill general bacterial propagules; using a potassium iodide solution containing 10 mg / L of available iodine for 30 seconds can kill Staphylococcus aureus.

[0019] The current related products on the market only have the effect of rapid sterilization. However, the present invention not only has the effect of rapid sterilization, but also has the effect of continuous sterilization, and the overall effect will be better.

[0020] The present invention is to prepare a micro-nano zinc pre-medicated dipping disinfectant for dairy cows, which is practically non-toxic, quickly sterilizes, has no irritation to the mucous membrane, and is ready for the clean treatment before milking.

[0021] The micro-nano zinc pre-dip disinfectant for dairy cows of the present invention can quickly kill pathogenic microorganisms on the nipples, prevent pathogenic microorganisms from contaminating the milk lining, and block the spread of pathogenic microorganisms. It can clean the nipples and prevent the nipple skin from being dry, frostbitten or chapped. The bactericidal components should be released quickly, and the bactericidal effect should be eliminated before the milk pores of dairy cows are opened, so as to prevent the occurrence of mastitis and papillitis. DETAILED DESCRIPTION

[0022] The present invention will be further explained below in conjunction with specific implementation modes. It should be understood that the following specific implementation modes are only used to illustrate the present invention and are not used to limit the scope of the present invention.

[0023] Example 1

[0024] S01: Add 3g of zinc gluconate to 500ml of 3000ppm micro-nano zinc, heat to 30℃-35℃ in a beaker, stir for 2h, add 0.05g of sodium hyaluronate, continue stirring for 1h, cool, and obtain a precursor solution;

[0025] S02 Take 300ml of precursor liquid, add 3g of potassium iodate, 6g of glycerol, 0.5g of vitamin E, and 200ml of deionized water, stir for 3h at a temperature of 65℃~70℃, and then cool to obtain the pre-drug bath disinfectant.

[0026] The preparation method of micro-nano zinc is the same as patent 2021107358455.

[0027] 1. Equipment

[0028] 1. Sample: Dilute the pre-dip disinfectant 40 times;

[0029] 2. Test bacteria: Escherichia coli, Staphylococcus aureus, Candida albicans;

[0030] 3. Neutralizer: 1% lecithin, 1% Tween-80TSB;

[0031] 4. Organic interferences: 3% bovine serum albumin;

[0032] 5. Constant temperature incubator (model: 404L);

[0033] 6. Culture medium: ordinary nutrient agar;

[0034] 7. Adjustable constant temperature water bath (model: SD28R-30-A12Y);

[0035] 8. Standard hard water: hardness 342 mg / L.

[0036] 2. Methods

[0037] 1. Inspection basis: Technical Specifications for Disinfection, 2002 edition, 2.1.1.5.5, 2.1.1.7.4;

[0038] 2. Neutralizer identification test: Test bacteria: Escherichia coli, Staphylococcus aureus, Candida albicans;

[0039] Disinfectant concentration: 1:40 Action time: 2min;

[0040] The test temperature was 20°C and the experiment was repeated 3 times.

[0041] 3. Experimental Results

[0042] The experimental results are shown in Table 1 below.

[0043] Table 1 Pre-medicated bath disinfectant test results

[0044]

[0045] The rest is the same as Example 1, except that zinc gluconate is not added, and the other components and processes remain unchanged. The test results of the prepared pre-drug bath disinfectant are shown in Table 2:

[0046] Table 2 Pre-drug bath disinfectant test results

[0047]

[0048] Example 2

[0049] S01: Add 10g of zinc gluconate to 500ml of 3000ppm micro-nano zinc, heat to 20℃-25℃ in a beaker, stir for 4h, add 0.5g of sodium hyaluronate, continue stirring for 2h, cool, and obtain a precursor solution;

[0050] S02 Take 300ml of precursor liquid, add 15g of potassium iodate, add 30g of glycerol, add 1.5g of vitamin E, add 200ml of deionized water, stir for 2h at a temperature of 50℃~55℃, and cool to obtain the pre-drug bath disinfectant.

[0051] The preparation method of micro-nano zinc is the same as patent 2021107358455.

[0052] 1. Equipment

[0053] 1. Sample: Dilute the pre-dip disinfectant 20 times;

[0054] 2. Test bacteria: Escherichia coli, Staphylococcus aureus, Candida albicans;

[0055] 3. Neutralizer: 1% lecithin, 1% Tween-80TSB;

[0056] 4. Organic interferences: 3% bovine serum albumin;

[0057] 5. Constant temperature incubator (model: 404L);

[0058] 6. Culture medium: ordinary nutrient agar;

[0059] 7. Adjustable constant temperature water bath (model: SD28R-30-A12Y);

[0060] 8. Standard hard water: hardness 342 mg / L.

[0061] 2. Methods

[0062] 1. Inspection basis: Technical Specifications for Disinfection, 2002 edition, 2.1.1.5.5, 2.1.1.7.4;

[0063] 2. Neutralizer identification test: Test bacteria: Escherichia coli, Staphylococcus aureus, Candida albicans;

[0064] Disinfectant concentration: 1:20 Action time: 2min;

[0065] The test temperature was 20°C and the experiment was repeated 3 times.

[0066] 3. Experimental Results

[0067] The experimental results are shown in Table 3 below.

[0068] Table 3 Pre-medicated bath disinfectant test results

[0069]

[0070] The rest is the same as Example 2, except that sodium hyaluronate is not added, and the other components and processes remain unchanged. The test results of the prepared pre-drug bath disinfectant are shown in Table 4

[0071] Table 4 Pre-medicated bath disinfectant test results

[0072]

[0073] Example 3

[0074] S01: Add 7.5g of zinc gluconate to 500ml of 3000ppm micro-nano zinc, heat to 45℃-50℃ in a beaker, stir for 3h, add 0.02g of sodium hyaluronate, continue stirring for 1.5h, cool, and obtain a precursor solution;

[0075] S02 Take 300ml of precursor liquid, add 10g of potassium iodate, 15g of glycerol, 1g of vitamin E, and 200ml of deionized water, stir for 3h at 80℃~85℃, and then cool to obtain the pre-drug bath disinfectant.

[0076] The preparation method of micro-nano zinc is the same as patent 2021107358455.

[0077] 1. Equipment

[0078] 1. Sample: Dilute the pre-dip disinfectant 30 times;

[0079] 2. Test bacteria: Escherichia coli, Staphylococcus aureus, Candida albicans;

[0080] 3. Neutralizer: 1% lecithin, 1% Tween-80TSB;

[0081] 4. Organic interferences: 3% bovine serum albumin;

[0082] 5. Constant temperature incubator (model: 404L);

[0083] 6. Culture medium: ordinary nutrient agar;

[0084] 7. Adjustable constant temperature water bath (model: SD28R-30-A12Y);

[0085] 8. Standard hard water: hardness 342 mg / L.

[0086] 2. Methods

[0087] 1. Inspection basis: Technical Specifications for Disinfection, 2002 edition, 2.1.1.5.5, 2.1.1.7.4;

[0088] 2. Neutralizer identification test: Test bacteria: Escherichia coli, Staphylococcus aureus, Candida albicans;

[0089] Disinfectant concentration: 1:30 Action time: 2min;

[0090] The test temperature was 20°C and the experiment was repeated 3 times.

[0091] 3. Experimental Results

[0092] The experimental results are shown in Table 5 below.

[0093] Table 5 Pre-medicated bath disinfectant test results

[0094]

[0095] The rest is the same as in Example 3, except that potassium iodate is not added, and the other components and processes remain unchanged. The test results of the prepared pre-drug bath disinfectant are shown in Table 6:

[0096] Table 6 Pre-medicated bath disinfectant test results

[0097]

[0098] Example 4

[0099] S01: Add 10g of zinc gluconate to 500ml of 3000ppm micro-nano zinc, heat to 45℃-50℃ in a beaker, stir for 2.5h, add 0.03g of sodium hyaluronate, continue stirring for 2h, cool, and obtain a precursor solution;

[0100] S02 Take 300ml of precursor liquid, add 12g of potassium iodate, 10g of glycerol, 1.2g of vitamin E, and 200ml of deionized water, stir for 2h at a temperature of 70℃~55℃, and then cool to obtain the pre-drug bath disinfectant.

[0101] The preparation method of micro-nano zinc is the same as patent 2021107358455.

[0102] 1. Equipment

[0103] 1. Sample: Dilute the pre-dip disinfectant 30 times;

[0104] 2. Test bacteria: Escherichia coli, Staphylococcus aureus, Candida albicans;

[0105] 3. Neutralizer: 1% lecithin, 1% Tween-80TSB;

[0106] 4. Organic interferences: 3% bovine serum albumin;

[0107] 5. Constant temperature incubator (model: 404L);

[0108] 6. Culture medium: ordinary nutrient agar;

[0109] 7. Adjustable constant temperature water bath (model: SD28R-30-A12Y);

[0110] 8. Standard hard water: hardness 342 mg / L.

[0111] 2. Methods

[0112] 1. Inspection basis: Technical Specifications for Disinfection, 2002 edition, 2.1.1.5.5, 2.1.1.7.4;

[0113] 2. Neutralizer identification test: Test bacteria: Escherichia coli, Staphylococcus aureus, Candida albicans;

[0114] Disinfectant concentration: 1:25 Action time: 2min;

[0115] The test temperature was 20°C and the experiment was repeated 3 times.

[0116] 3. Experimental Results

[0117] The experimental results are shown in Table 7 below.

[0118] Table 7 Pre-medicated bath disinfectant test results

[0119]

[0120] The rest is the same as Example 4, except that glycerol and vitamin E are not added, and other components and processes remain unchanged. The test results of the prepared prodrug bath disinfectant are shown in Table 8:

[0121] Table 8 Pre-medicated bath disinfectant test results

[0122]

[0123]

[0124] The pre-drug bath disinfectant prepared in Example 4 and the factory inspection report are shown in Table 9.

[0125] Table 9 Pre-factory inspection of medicated bath disinfectant

[0126]

[0127] In order to verify the preventive effect of pre-dip disinfectant on mastitis and papillitis in dairy cows, we conducted a 20-day pre-dip verification test at a group ranch in Inner Mongolia.

[0128] Experimental method: 1. According to the grouping of dairy cows on the ranch, cows in good health were selected and divided into experimental group and control group.

[0129] 2. The control group was a blank group, with 90 cows, and no pre-dip disinfectant was used;

[0130] 3. The 90 cattle in the experimental group were treated with pre-dip disinfectant diluted 1:40 and given two pre-dip baths a day with the same interval between each bath.

[0131] Test results: The pre-medicated bath disinfectant has a very good preventive effect on mastitis, with a prevention rate of 97.78%, and a prevention rate of 95.56% for teatitis.

[0132] Table 10 Pre-medicated dip disinfectant, results of the test on the prevention of mastitis and papillitis

[0133]

[0134] It should be noted that the above content merely illustrates the technical idea of ​​the present invention and cannot be used to limit the protection scope of the present invention. For ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principles of the present invention. These improvements and modifications are all protected by the claims of the present invention.

Claims

1. A method for preparing a pre-dip disinfectant for dairy farms, characterized in that: The following steps are involved: Add zinc gluconate to S01 micro-nano zinc, heat to 20°C-50°C, stir for 2h-4h, add sodium hyaluronate, continue stirring for 1h-2h, cool, and prepare a precursor solution; Potassium iodate, glycerol, vitamin E and deionized water are added to the S02 precursor solution, stirred at a temperature of 45°C to 65°C for 1h to 4h, and then cooled to obtain a pre-drug bath disinfectant solution.

2. The method for preparing a pre-dip disinfectant for dairy farms according to claim 1, characterized in that: In step S01, the mass ratio of micro-nano zinc to zinc gluconate is 200:1 to 50:

1.

3. The method for preparing a pre-medicated disinfectant for dairy farms according to claim 1, characterized in that: In step S01, the mass ratio of micro-nano zinc to sodium hyaluronate is 20000:1 to 1000:

1.

4. The method for preparing a pre-medicated disinfectant for dairy farms according to claim 1, characterized in that: In step S02, the mass ratio of the precursor solution to potassium iodate is 100:1 to 10:

1.

5. The method for preparing a pre-dip disinfectant for dairy farms according to claim 1, characterized in that: In step S02, the mass ratio of potassium iodate, glycerol, vitamin E, and deionized water is 1-10: 1-15: 0.1-2: 73-97.9.

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