Damascus rose detoxification rapid propagation method

Abstract

The invention relates to the technical field of damascene rose detoxification and rapid propagation, and discloses a damascene rose detoxification and rapid propagation method which comprises the following steps: an earlier-stage preparation stage: collecting damascene rose stock plants for pruning pretreatment, and preparing heat treatment equipment, a chemical agent solution and materials required by rapid propagation at the same time, in the detoxification treatment stage, a Damascus rose stock plant is placed in an artificial climate box for heat treatment detoxification, after heat treatment detoxification, the Damascus rose stock plant is sheared into cutting slips, and the cutting slips are placed in a chemical agent solution for chemical agent soaking detoxification, and in the rapid propagation operation stage, the cutting slips are soaked in a rooting agent for 2-4 hours, and air-drying the soaked cutting slips. According to the method, heat treatment detoxification and chemical agent detoxification are combined, so that the virus content in Damascus rose plants or cuttings can be effectively reduced, and the problem that the yield is not high due to the fact that single heat treatment detoxification is usually adopted in a traditional detoxification step is solved.

Description

Technical Field

[0001] The invention relates to the technical field of Damascus rose virus-free rapid propagation, in particular to a Damascus rose virus-free rapid propagation method. Background Art

[0002] Damascus rose is a shrub of the genus Rosa in the family Rosaceae, also known as Bulgarian rose. The plant is about 2 meters tall, with twigs with thick hooked prickles and usually 5 leaflets. The flowers are arranged in 6-12 corymbs, with a diameter of 3-5 cm and pink petals. The fruit is pear-shaped or obovoid, red and often with prickles. It is suitable for growing in a continental climate with distinct four seasons. Its flowers can be used to extract rose essential oil, etc., and have high economic and ornamental value.

[0003] Traditional detoxification steps often use a single heat treatment for detoxification, which may result in incomplete detoxification and easy virus recurrence in the subsequent rapid propagation process, resulting in a low yield rate problem. Summary of the invention

[0004] In view of the shortcomings of the prior art, the present invention provides a method for detoxification and rapid propagation of Damascus rose, which solves the problem that the traditional detoxification step often adopts a single heat treatment detoxification, which may cause incomplete detoxification and easy virus recurrence in the subsequent rapid propagation process, thereby resulting in a low yield rate.

[0005] To achieve the above objectives, the present invention is implemented by the following technical scheme: A method for rapid propagation of Damascus roses by virus removal, comprising the following steps:

[0006] S1. Preliminary preparation stage: Collect Damascus rose mother plants for pruning pretreatment, and prepare heat treatment equipment, chemical solution and materials required for rapid propagation;

[0007] S2, detoxification treatment stage: placing the Damascus rose mother plant in an artificial climate box for heat treatment and detoxification, cutting the Damascus rose mother plant into cuttings after the heat treatment and detoxification, and placing the cuttings in a chemical agent solution for chemical agent soaking and detoxification;

[0008] S3, rapid propagation operation stage: soak the cuttings in the rooting agent for 2-4 hours, air-dry the cuttings after soaking, and then insert the cuttings into the cutting medium;

[0009] S4, Subsequent management stage: adjust the temperature, humidity and light conditions of the cutting environment as needed;

[0010] S5. Detection stage: virus detection is performed on the plants after cutting.

[0011] Preferably, the straight diameter of the Damascus rose mother plant in S1 is 0.5-1 cm, the length is 30-50 cm, the color of the branch is dark green, and the surface is smooth; the length of the leaf is 5-10 cm, the width is 3-6 cm, the shape of the leaf is oval and oval-oblong, the edge serrations are clear and regular, there are no spots, yellowing and withered parts on the leaf surface, and there are no wormholes or mildew spots on the back; the plant is shrub-like and has a height of 1.5-2 meters; the flower branch has 3-5 flowers, the flower diameter is 3-5 cm, and the number of petals is 30-40; the root color is white or light yellow, and the main root length is 30-50 cm.

[0012] Preferably, the artificial climate box in S2 is set at a temperature of 38-42° C. and a relative humidity of 60-70% for 2-3 weeks.

[0013] Preferably, the cuttings in S2 are 10-15 cm long, and each cutting has 2-3 buds on its outer wall.

[0014] Preferably, the chemical solution in S2 includes the following raw materials in percentage: 0.01%-0.03% of triazole ribavirin, 0.015%-0.025% of ningnanmycin, 0.25%-0.33% of phytopathogen and 99.615%-99.725% of pure water. The soaking requires that the chemical solution 100% soaks the cuttings, and the soaking is maintained for 1-2 hours. After the soaking is completed, the cuttings are rinsed in pure water with a flow rate of 0.5 meters per second for 2-4 minutes.

[0015] Preferably, in the air drying in S3, the cuttings are placed in an air dryer, the wind speed is controlled to be 0.5-1.5 meters per second, the temperature is 20-25° C., the humidity is 40%-60%, and the air drying time is 1-3 hours.

[0016] Preferably, in said S3, the cuttings are inserted into the cutting medium to a depth of 1 / 3-1 / 2 of the length of the cuttings, and the ambient temperature of the cutting medium into which the cuttings are inserted is controlled at 20-25° C. and the relative humidity is 70-80%.

[0017] Preferably, the cutting environment in S4 is adjusted as needed, and the light intensity is controlled at 1000-3000 lux, the temperature is maintained at 20-25°C, and the relative humidity is maintained at 80%-90% 1-2 weeks after cutting; the light intensity is controlled at 3000-5000 lux, the temperature is maintained at 22-27°C, and the relative humidity is maintained at 70%-80% 3-4 weeks after cutting; the light intensity is controlled at 5000-10000 lux, the temperature is maintained at 20-28°C, and the relative humidity is maintained at 60%-70% after 5 weeks of cutting.

[0018] The present invention provides a method for rapid propagation of Damascus roses, which has the following beneficial effects:

[0019] 1. In the present invention, the virus content in Damascus rose plants or cuttings can be effectively reduced by combining heat treatment detoxification with chemical agent detoxification. Heat treatment inactivates the virus at high temperature. The triazole ribavirin in the chemical agent interferes with viral nucleic acid synthesis, Ningnanmycin inhibits viral RNA synthesis, and the multi-components of phytopathogens exert antiviral effects. This not only improves the quality of the propagation material, but also enhances the rooting ability of the cuttings after detoxification, thereby improving the traditional detoxification step that often uses a single heat treatment detoxification, which may cause incomplete detoxification, and the virus is easy to recur in the subsequent rapid propagation process, thereby resulting in a low yield rate.

[0020] 2. In the present invention, the light intensity is controlled at 1000-3000 lux, the temperature is maintained at 20-25°C, and the relative humidity is maintained at 80%-90% 1-2 weeks after cutting; the light intensity is controlled at 3000-5000 lux, the temperature is maintained at 22-27°C, and the relative humidity is maintained at 70%-80% 3-4 weeks after cutting; the light intensity is controlled at 5000-10000 lux, the temperature is maintained at 20-28°C, and the relative humidity is maintained at 60%-70% 5 weeks after cutting. The whole process improves the success rate and breeding quality of Damascus rose cuttings by accurately regulating environmental factors in stages. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] Figure 1 It is an operation flow chart of the present invention. DETAILED DESCRIPTION

[0022] The technical solution of the present invention will be described clearly and completely below in conjunction with the accompanying drawings of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0023] Please refer to the attached Figure 1 The embodiment of the present invention provides a method for rapid propagation of Damascus rose by virus removal, comprising the following steps:

[0024] S1. Preliminary preparation stage: Collect Damascus rose mother plants for pruning pretreatment, and prepare heat treatment equipment, chemical solution and materials required for rapid propagation;

[0025] S2, detoxification treatment stage: placing the Damascus rose mother plant in an artificial climate box for heat treatment and detoxification, cutting the Damascus rose mother plant into cuttings after the heat treatment and detoxification, and placing the cuttings in a chemical agent solution for chemical agent soaking and detoxification;

[0026] S3, rapid propagation operation stage: soak the cuttings in the rooting agent for 2-4 hours, air-dry the cuttings after soaking, and then insert the cuttings into the cutting medium;

[0027] S4, Subsequent management stage: adjust the temperature, humidity and light conditions of the cutting environment as needed;

[0028] S5. Detection stage: virus detection is performed on the plants after cutting.

[0029] Specifically, the cutting medium is a mixture of vermiculite, perlite, peat soil and coconut bran, and the mixing ratio is 2:2:3:3. The texture of the mixture is neither too loose, resulting in the inability to fix the cuttings, nor too compact, affecting the growth of the root system. Therefore, the cuttings can be well wrapped, providing stable support for the cuttings, so that they can remain upright during the rooting process. Vermiculite can absorb several times its own weight of water, and the fiber structure of coconut bran can also absorb and retain a large amount of water, so that the cutting medium can provide a continuous supply of water for the cuttings, especially in the early stage of cutting. The cuttings have a high demand for water before taking root, and good water retention can prevent the cuttings from being damaged due to lack of water. The porous structure of perlite can make the cutting medium have good air permeability, which can quickly discharge excess water and avoid water accumulation in the matrix. At the same time, there are many gaps between the vermiculite particles, which can further improve the air permeability. Good air permeability can provide sufficient oxygen for the respiration of the cuttings during the rooting process, which is conducive to the healthy development of the root system and prevents root rot caused by water accumulation. Peat soil is rich in organic matter, such as nitrogen, phosphorus, potassium and other nutrients. Through peat soil, these nutrients can be gradually absorbed and utilized after the cuttings take root, providing necessary nutritional support for the growth of the cuttings, and promoting the further growth of the root system and the germination of new shoots.

[0030] The straight diameter of the Damascus rose mother plant in S1 is 0.5-1 cm, the length is 30-50 cm, the branch color is dark green, and the surface is smooth; the leaf length is 5-10 cm, the width is 3-6 cm, the leaf shape is oval and oval-oblong, the edge serrations are clear and regular, there are no spots, yellowing or withered parts on the leaf surface, and there are no wormholes or mildew spots on the back; the plant is shrub-like, with a height of 1.5-2 meters; the flower branch has 3-5 flowers, the flower diameter is 3-5 cm, and the number of petals is 30-40; the root color is white or light yellow, and the main root length is 30-50 cm.

[0031] Specifically, Damascus rose mother plant branches with a diameter of 0.5-1 cm, a length of 30-50 cm, dark green color, and a smooth surface are selected for virus-free rapid propagation. Such branches indicate that the mother plant is growing robustly and has rich nutrients stored inside, which can provide sufficient energy for the virus-free treatment and rapid propagation process. The dark green branches have high chlorophyll content and strong photosynthesis, which can provide a good foundation for subsequent growth. The smooth surface can reflect that the branches are healthy and free of disease and pest infection, reduce interference factors in the virus-free process, improve the success rate of virus-free and the quality of rapid propagation, and are conducive to cultivation. Healthy and high-quality Damascus rose plants can be produced; by selecting leaves of moderate size and regular shape, it indicates that the mother plant is growing well and has a normal physiological state. Clear and regular serrations on the edge can reflect the good development of the plant, which is conducive to photosynthesis and gas exchange. There are no spots, yellowing and withering on the surface of the leaves. By selecting leaves without insect holes and mildew on the back, it can ensure that the mother plant is not infected by pests and diseases, reduce interference factors in the detoxification process, and further improve the success rate of detoxification. Such leaves can better photosynthesize and provide sufficient energy for plant growth, which is beneficial for subsequent rapid propagation and The plant is shrub-like and 1.5-2 meters in height, which can reflect the good growth trend of the plant and the stable growth structure. Each flower branch has 3-5 flowers, the flower diameter is 3-5 cm, and the petals are 30-40, which can reflect the normal reproductive growth and the excellent characteristics of the variety. This shows that the mother plant is in good condition in terms of nutrient supply and hormone regulation, which can provide healthy and high-quality materials for virus-free rapid propagation, and is conducive to cultivating virus-free plants with good growth and flowering performance, and for the subsequent breeding of high-quality Damascus rose seeds. The main root length of 30-50 cm can ensure that the mother plant is firmly rooted in the soil and its lodging resistance is enhanced. At the same time, it can also reflect that the root system has a strong ability to explore and absorb soil resources, so that the mother plant can accumulate sufficient nutrient reserves during the growth process, laying a good foundation for the growth and development of the plant during the process of virus removal and rapid propagation, and further improving the success rate of virus removal and the quality of rapid propagation.

[0032] The artificial climate box in S2 is set at a temperature of 38-42°C and a relative humidity of 60-70% for 2-3 weeks. The length of the cuttings in S2 is 10-15 cm, and there are 2-3 buds on the outer wall of each cutting. The chemical solution in S2 includes the following raw materials in percentage: 0.01%-0.03% of triazole ribavirin, 0.015%-0.025% of ningnanmycin, 0.25%-0.33% of plant disease killer and 99.615%-99.725% of pure water. The soaking requires that the chemical solution 100% soaks the cuttings, and the soaking is maintained for 1-2 hours. After the soaking is completed, it is rinsed in pure water with a flow rate of 0.5 meters per second for 2-4 minutes.

[0033] Specifically, by setting the temperature of the artificial climate box at 38-42°C and the relative humidity at 60-70%, it can help inhibit the activity of viruses and avoid excessive damage to plants. The length of the cuttings is 10-15 cm, which can store enough nutrients for consumption in the initial stage of rooting. Each cutting has 2-3 buds on the outer wall, which can produce auxin to promote cell division and differentiation at the base of the cuttings, induce adventitious root formation, and improve the rooting rate. The synthesis of viral nucleic acid can be interfered with by triazole riboside, the synthesis of viral RNA can be inhibited by ningnanmycin, and the antiviral effect of plant disease killer can be exerted from a multi-component perspective. After soaking, the cuttings are rinsed in pure water with a flow rate of 0.5 meters per second for 2-4 minutes, thereby preventing the remaining chemicals from causing unnecessary effects on subsequent rapid propagation. The detoxification effect can be achieved to the greatest extent by combining heat treatment and subsequent chemical detoxification in the artificial climate box, thereby making the subsequent rapid propagation faster.

[0034] In the air drying in S3, the cuttings are placed in an air dryer, the wind speed is controlled to be 0.5-1.5 meters per second, the temperature is 20-25°C, the humidity is 40%-60%, and the air drying time is 1-3 hours. In S3, the cuttings are inserted into the cutting medium, the insertion depth is 1 / 3-1 / 2 of the length of the cuttings, and the ambient temperature of the cutting medium where the cuttings are inserted is controlled at 20-25°C, and the relative humidity is 70-80%.

[0035] Specifically, by placing the cuttings into an air dryer, controlling the wind speed to 0.5-1.5 meters per second, the temperature to 20-25°C, and the humidity to 40%-60%, the cuttings can maintain physiological activity and reduce the risk of pathogen growth. The air drying time of 1-3 hours can enable the cuttings to reach a suitable state for insertion into the cutting medium, improve the survival rate and rooting quality of the cuttings, and provide strong guarantees for the rapid propagation of Damascus roses. By inserting the cuttings to a depth of 1 / 3-1 / 2 of the length of the cuttings, the cuttings can be stably rooted and the normal growth and development of the buds can be ensured. By maintaining the temperature at 20-25°C, the normal physiological activities of the cuttings can be maintained, and cell division and growth can be promoted. By controlling the relative humidity to 70-80%, a suitable moisture environment can be provided for the cuttings, reducing the risk of water evaporation and drying of the cuttings, which is conducive to wound healing and rooting of the cuttings, and improving the survival rate and rapid propagation effect of the cuttings, creating good conditions for the rapid propagation of Damascus roses.

[0036] In S4, the cutting environment is adjusted as needed. In the 1-2 weeks after cutting, the light intensity is controlled at 1000-3000 lux, the temperature is maintained at 20-25℃, and the relative humidity is maintained at 80%-90%; in the 3-4 weeks after cutting, the light intensity is controlled at 3000-5000 lux, the temperature is maintained at 22-27℃, and the relative humidity is maintained at 70%-80%; 5 weeks after cutting, the light intensity is controlled at 5000-10000 lux, the temperature is maintained at 20-28℃, and the relative humidity is maintained at 60%-70%.

[0037] Specifically, controlling the light intensity at 1000-3000 lux, maintaining the temperature at 20-25°C, and maintaining the relative humidity at 80%-90% 1-2 weeks after cutting can help the wound healing and rooting of the cuttings, prevent the cuttings from drying out due to water loss, and create a mild environment for the initial rooting stage; controlling the light intensity at 3000-5000 lux, maintaining the temperature at 22-27°C, and maintaining the relative humidity at 70%-80% 3-4 weeks after cutting can promote the growth of new roots and the germination of new shoots after the cuttings take root, and enhance photosynthesis and nutrient absorption; controlling the light intensity at 5000-10000 lux, maintaining the temperature at 20-28°C, and maintaining the relative humidity at 60%-70% 5 weeks after cutting can make the cuttings adapt to the natural environment, make the plants stronger, and prepare for transplanting, thereby ensuring precise control of environmental factors in stages, thereby improving the survival rate and reproduction quality of Damascus rose rapid propagation.

[0038] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A method for rapid propagation of Rosa damask, characterized in that: The following steps are involved: S1. Preliminary preparation stage: Collect Damascus rose mother plants for pruning pretreatment, and prepare heat treatment equipment, chemical solution and materials required for rapid propagation; S2, detoxification treatment stage: placing the Damascus rose mother plant in an artificial climate box for heat treatment and detoxification, cutting the Damascus rose mother plant into cuttings after the heat treatment and detoxification, and placing the cuttings in a chemical agent solution for chemical agent soaking and detoxification; S3, rapid propagation operation stage: soak the cuttings in the rooting agent for 2-4 hours, air-dry the cuttings after soaking, and then insert the cuttings into the cutting medium; S4, Subsequent management stage: adjust the temperature, humidity and light conditions of the cutting environment as needed; S5. Detection stage: virus detection is performed on the plants after cutting.

2. A method for rapid propagation of Rosa damask by virus removal according to claim 1, characterized in that: The Damascus rose mother plant in S1 has a straight bar diameter of 0.5-1 cm and a length of 30-50 cm. The branch color is dark green and the surface is smooth; the leaf length is 5-10 cm and the width is 3-6 cm. The leaf shape is oval and oval-oblong, and the edge serrations are clear and regular. There are no spots, yellowing or withered parts on the leaf surface, and there are no wormholes or mold spots on the back. The plant is shrub-like and has a height of 1.5-2 meters. The flower branch has 3-5 flowers, the flower diameter is 3-5 cm, and the number of petals is 30-40; the root color is white or light yellow, and the main root length is 30-50 cm.

3. A method for rapid propagation of Rosa damask by virus removal according to claim 2, characterized in that: The artificial climate box in S2 is set at a temperature of 38-42°C and a relative humidity of 60-70% for 2-3 weeks.

4. A method for rapid propagation of Rosa damask by virus removal according to claim 3, characterized in that: The cuttings in S2 are 10-15 cm long, and each cutting has 2-3 buds on its outer wall.

5. A method for rapid propagation of Rosa damask by virus removal according to claim 3, characterized in that: The chemical solution in S2 includes the following raw materials in percentage: 0.01%-0.03% of triazole ribavirin, 0.015%-0.025% of ningnanmycin, 0.25%-0.33% of phytopathogen and 99.615%-99.725% of pure water. The soaking requires that the cuttings are 100% immersed in the chemical solution and kept immersed for 1-2 hours. After the soaking is completed, rinse in pure water with a flow rate of 0.5 meters per second for 2-4 minutes.

6. A method for rapid propagation of Rosa damaskii by virus removal according to claim 3, characterized in that: The air drying in S3 is to put the cuttings into an air dryer, control the wind speed to 0.5-1.5 meters per second, the temperature to 20-25° C., the humidity to 40%-60%, and the air drying time to 1-3 hours.

7. The method for rapid propagation of Rosa damaskii by virus removal according to claim 1, characterized in that: In S3, the cuttings are inserted into the cutting medium, the insertion depth of which is 1 / 3-1 / 2 of the length of the cuttings, and the environmental temperature of the cutting medium into which the cuttings are inserted is controlled at 20-25° C. and the relative humidity is 70-80%.

8. The method for rapid propagation of Rosa damaskii according to claim 1, characterized in that: The cutting environment in S4 is adjusted as needed, and the light intensity is controlled at 1000-3000 lux, the temperature is maintained at 20-25°C, and the relative humidity is maintained at 80%-90% 1-2 weeks after cutting; the light intensity is controlled at 3000-5000 lux, the temperature is maintained at 22-27°C, and the relative humidity is maintained at 70%-80% 3-4 weeks after cutting; the light intensity is controlled at 5000-10000 lux, the temperature is maintained at 20-28°C, and the relative humidity is maintained at 60%-70% after 5 weeks of cutting.

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