Polypeptide cracking system and operation method thereof

Abstract

The invention belongs to the technical field of polypeptide cracking, and discloses a polypeptide cracking system which comprises a 50L cylindrical cone type cracking reaction kettle, a 500L settling kettle, a 100L drying kettle, an alarm recording module, a user management module, a system information module and a logout module. And the 500L sedimentation kettle is a vertical container made of 316L outer material and 316L inner material coated with PFA (Polyfluoroalkoxy) stainless steel. According to the invention, the splitting work of a large amount of polypeptide can be completed in a short time, the experiment efficiency is obviously improved, the advanced computer control system is adopted, the rapid and accurate splitting of the polypeptide can be realized, the manual operation is reduced, the experiment precision and efficiency are improved, and the high-purity splitting product can be generated by the polypeptide splitting system by accurately controlling the splitting condition; the workload of subsequent purification and separation is reduced, the polypeptide cracking system has good repeatability, the stability and consistency of each experiment result can be ensured, and the reliability and accuracy of the experiment are improved.

Description

Technical Field

[0001] The invention belongs to the technical field of polypeptide cleavage, and in particular relates to a polypeptide cleavage system and an operation method thereof. Background Art

[0002] Peptides are a type of biological macromolecule composed of amino acids connected by peptide bonds. They play important structural and functional roles in organisms. Since peptides have a wide range of biological activities and application potentials, such as drugs, enzyme inhibitors, biosensors, etc., peptide cleavage technology has received extensive attention and application in the fields of new drug development, biochemical research and biotechnology.

[0003] Peptide cleavage technology mainly uses specific chemical or physical methods to break the polypeptide chain at a specific position to obtain short peptides or amino acid fragments with specific sequences and functions. This technology is of great significance for analyzing the structure and functional relationship of polypeptides and developing new polypeptide drugs.

[0004] Although the existing peptide cleavage technology has made significant progress in the field of biochemical research and drug development, it still has some shortcomings that cannot be ignored. First, chemical cleavage methods such as acid hydrolysis and alkaline hydrolysis often lead to non-specific breakage of polypeptide chains, making it difficult to accurately control the cleavage position, thereby increasing the difficulty of subsequent purification and identification. Therefore, it needs to be modified and optimized. Summary of the invention

[0005] The object of the present invention is to provide a polypeptide cleavage system and an operation method thereof to solve the problems raised in the above background technology.

[0006] In order to achieve the above object, the present invention provides the following technical solutions: a polypeptide lysis system, which is composed of: a 50L cylindrical cone lysis reactor, a 500L sedimentation reactor, a 100L drying reactor, an alarm recording module, a user management module, a system information module and a logout module;

[0007] The 500L sedimentation kettle is a vertical container made of 316L outer material and 316L inner material, sprayed with PFA stainless steel.

[0008] The 500L sedimentation kettle is a vertical container made of 316L outer material and 316L inner material sprayed with PFA stainless steel;

[0009] The 100L drying kettle is a vertical container made of 316L outer material and 316L inner material sprayed with PFA stainless steel.

[0010] Preferably, the operating steps of the system are:

[0011] A1, Inspection: Check the electrical control connection, circuit, pipe opening and pipeline sealing, equipment air tightness, measure the pressure of each gas source port, and focus on the connection and sealing between the lower cone and the cylinder of the reactor;

[0012] A2, Account login: The administrator, engineer, operator and monitor login accounts are authorized respectively;

[0013] A3, Startup preparation: After checking that everything is correct, press the start button in the equipment control cabinet to start the equipment;

[0014] A4, Operation: After logging in to the interface, operate the system.

[0015] Preferably, in said A2;

[0016] Administrator privileges are first-level management privileges: can log in to the highest authorized control device, can modify any instrument parameters, can control the switch and stop of pumps and valves;

[0017] Engineers are the second-level permission group: they have the authority to set and modify parameters;

[0018] The operator authority is a three-level authority group: only the start and stop authority of the pump and valve, and the start and stop authority of the button interlock;

[0019] The supervisor authority is the driver authority group: only has the supervisor authority.

[0020] Preferably, the startup preparation in A3 is divided into:

[0021] B1, turn on the power supply; turn on the nitrogen and compressed air sources;

[0022] B2, check the control cabinet; check the aging of the power supply and components of the control cabinet;

[0023] B3, Check the instrument: Check the instrument collection values ​​and system parameters;

[0024] B4, check the solenoid valve: check the solenoid valves and pumps for stuck phenomenon.

[0025] Preferably, the operations in A4 are divided into: interface login, process operation, parameter setting, alarm record, user management, system information query and system setting.

[0026] Preferably, the process operation is divided into: cleaning, MTBE liquid feeding, TFA feeding, cracking kettle discharging, draining, settling kettle liquid transfer, and discharging.

[0027] Preferably, the cleaning steps are: click the cleaning button, select the cleaning amount and the nitrogen purge seconds, and click the kettle body to be cleaned;

[0028] The steps for MTBE and liquid injection are: click the liquid injection button, select the liquid injection volume and purge seconds, and select the kettle body to be injected;

[0029] The steps of TFA feeding are as follows: ① Click the liquid feeding button; select the liquid feeding volume and the purge seconds; click to start the liquid feeding, and click again to stop the liquid feeding;

[0030] The steps for discharging the cracking kettle are: click on the pop-up window of discharging the cracking kettle, select the container to be transferred into, and the corresponding valve will open automatically after clicking, and it will stop after clicking again.

[0031] Preferably, the step of draining liquid is to click on the pop-up window of three-in-one waste liquid discharge to start the discharge of the solution in the drying kettle, and click on the stop button to stop the discharge;

[0032] The steps of liquid transfer in the settling kettle are: click the bottom valve of the settling kettle, select start, pressurize the nitrogen in the settling kettle, and the solution in the settling kettle will be transferred into the three-in-one kettle through pressure transfer;

[0033] The steps of discharging are as follows: click the bottom valve at the lower end of the kettle body, and after starting, the eccentric butterfly valve at the bottom of the kettle body opens, and the solid material will fall naturally with gravity.

[0034] The beneficial effects of the present invention are as follows:

[0035] The present invention can significantly improve the experimental efficiency by completing the cleavage of a large number of polypeptides in a short time. The advanced computer control system is adopted to achieve rapid and accurate cleavage of polypeptides, reduce manual operations, and improve experimental accuracy and efficiency. By accurately controlling the cleavage conditions, the polypeptide cleavage system can produce high-purity cleavage products, reduce the workload of subsequent purification and separation, and the polypeptide cleavage system has good repeatability, which can ensure the stability and consistency of each experimental result and improve the reliability and accuracy of the experiment. BRIEF DESCRIPTION OF THE DRAWINGS

[0036] Figure 1 It is a schematic diagram of the operation flow of the present invention;

[0037] Figure 2 This is a schematic diagram of the pre-processing step A3 of the present invention. DETAILED DESCRIPTION

[0038] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0039] like Figure 1 to Figure 2As shown, an embodiment of the present invention provides a polypeptide lysis system, which consists of: a 50L cylindrical cone lysis reactor, a 500L sedimentation reactor, a 100L drying reactor, an alarm recording module, a user management module, a system information module and a logout module;

[0040] The 500L sedimentation kettle is a vertical container made of 316L outer material and 316L inner material sprayed with PFA stainless steel.

[0041] The 500L sedimentation kettle is a vertical container made of 316L outer material and 316L inner material sprayed with PFA stainless steel;

[0042] The 100L drying kettle is a vertical container made of 316L outer material and 316L inner material sprayed with PFA stainless steel;

[0043] By being able to complete the cleavage of a large number of peptides in a short period of time, the experimental efficiency is significantly improved. The use of advanced computer control systems can achieve rapid and accurate cleavage of peptides, reduce manual operations, and improve experimental accuracy and efficiency. By precisely controlling the cleavage conditions, the peptide cleavage system can produce high-purity cleavage products, reducing the workload of subsequent purification and separation. The peptide cleavage system has good repeatability, which can ensure the stability and consistency of each experimental result and improve the reliability and accuracy of the experiment.

[0044] The operating steps of the system are:

[0045] A1, Inspection: Check the electrical control connection, circuit, pipe opening and pipeline sealing, equipment air tightness, measure the pressure of each gas source port, and focus on the connection and sealing between the lower cone and the cylinder of the reactor;

[0046] A2, Account login: The administrator, engineer, operator and monitor login accounts are authorized respectively;

[0047] A3, Startup preparation: After checking that everything is correct, press the start button in the equipment control cabinet to start the equipment;

[0048] A4, Operation: After logging in to the interface, operate the system;

[0049] The inspection ensured that the peptide lysis system operated efficiently in a safe and controllable environment, providing accurate and reliable lysis effects for the experiment.

[0050] Among them, A2;

[0051] Administrator privileges are first-level management privileges: can log in to the highest authorized control device, can modify any instrument parameters, can control the switch and stop of pumps and valves;

[0052] Engineers are the second-level permission group: they have the authority to set and modify parameters;

[0053] The operator authority is a three-level authority group: only the start and stop authority of the pump and valve, and the start and stop authority of the button interlock;

[0054] The supervisor authority is the driver authority group: only has the supervisor authority.

[0055] The account login process of the polypeptide lysis system has a clear division of authority. The administrator has the highest level of management authority and can fully control the equipment, including modifying instrument parameters and controlling the switching on and off of pumps and valves to ensure efficient operation and precise control of the system. Engineers are granted level 2 authority and have the power to set and modify parameters to support the optimization and adjustment of the lysis process. Operators are in the level 3 authority group and are mainly responsible for starting and stopping pumps and valves and button interlocks to perform specific lysis operations. Monitors, as the driver authority group, only have monitoring authority and are responsible for real-time monitoring of the system's operating status to ensure the safety and stability of the lysis process. This authority division system improves the system's operational standardization and safety.

[0056] Among them, the startup preparation in A3 is divided into:

[0057] B1, turn on the power supply; turn on the nitrogen and compressed air sources;

[0058] B2, check the control cabinet; check the aging of the power supply and components of the control cabinet;

[0059] B3, Check the instrument: Check the instrument collection values ​​and system parameters;

[0060] B4, check the solenoid valve: check the solenoid valve and pump for stuck phenomenon;

[0061] Through the preparation before startup, the necessary power support is provided to the system, the stability and reliability of the control cabinet are ensured, and the accuracy of the values ​​collected by the instruments and the system parameters is confirmed to ensure the precise control of the cracking process. By checking whether the solenoid valves and pumps are stuck, the normal opening and closing of the valves and the smooth operation of the pumps are ensured. At the same time, a solid foundation is laid for the smooth startup and efficient operation of the system, and the stability and safety of the cracking process are improved.

[0062] Among them, the operations in A4 are divided into: interface login, process operation, parameter setting, alarm record, user management, system information query and system setting.

[0063] Log in to the system through the interface and enter the operation interface. Then, perform process operations according to experimental requirements. At the same time, the system allows users to set parameters to adjust various parameters in the cracking process to ensure the cracking effect. In addition, the system also has an alarm recording function, which can record and prompt abnormal situations that occur during equipment operation in real time. The user management function facilitates the authority management and operation record query of system users. The system information query and system setting functions provide users with conveniences such as equipment status query, historical data viewing and system configuration adjustment.

[0064] Among them, the process operations are divided into: cleaning, MTBE liquid feeding, TFA feeding, cracking kettle discharging, drainage, sedimentation kettle liquid transfer, and discharging.

[0065] Cleaning is performed to ensure the cleanliness of the equipment. MTBE liquid inlet and TFA feed are used to prepare the conditions for the cracking reaction. The discharge from the cracking kettle indicates the completion of the cracking. Then the liquid is discharged to clean up the residue. The liquid transfer in the sedimentation kettle promotes the separation of substances. Finally, the product is discharged. This series of operations ensures the orderly progress of the cracking process and the efficient output of the target product.

[0066] The cleaning steps are: click the cleaning button, select the cleaning volume and nitrogen purge seconds, and click the kettle body to be cleaned;

[0067] The steps for MTBE and liquid injection are: click the liquid injection button, select the liquid injection volume and purge seconds, and select the kettle body to be injected;

[0068] The steps for TFA feeding are: ① Click the liquid feeding button; select the liquid feeding volume and purge seconds; click to start the liquid feeding, and click again to stop the liquid feeding.

[0069] The steps for discharging the cracking kettle are: click on the pop-up window of discharging the cracking kettle, select the container to be transferred into, and the corresponding valve will open automatically after clicking, and it will stop after clicking again.

[0070] Among them, the step of draining liquid is to click on the pop-up window of three-in-one waste liquid discharge to select start, and the solution in the drying kettle will be discharged. Click the stop button to stop draining liquid;

[0071] The steps of liquid transfer in the settling kettle are: click the bottom valve of the settling kettle, select start, pressurize the nitrogen in the settling kettle, and the solution in the settling kettle will be transferred into the three-in-one kettle through pressure transfer;

[0072] The steps of discharging are as follows: click the bottom valve at the lower end of the kettle body, and after starting, the eccentric butterfly valve at the bottom of the kettle body opens, and the solid material will fall naturally with gravity.

[0073] It ensures the smooth transfer and processing of solutions and solids, and improves production efficiency and operational convenience.

[0074] It should be noted that, in this article, relational terms such as first and second, etc. are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Moreover, the terms "include", "comprise" or any other variants thereof are intended to cover non-exclusive inclusion, so that a process, method, article or device including a series of elements includes not only those elements, but also other elements not explicitly listed, or also includes elements inherent to such process, method, article or device.

[0075] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A polypeptide cleavage system, characterized in that: The system consists of: 50L cylindrical cone pyrolysis reactor, 500L sedimentation reactor, 100L drying reactor, alarm recording module, user management module, system information module and logout module; The 500L sedimentation kettle is a vertical container made of 316L outer material and 316L inner material, sprayed with PFA stainless steel. The 500L sedimentation kettle is a vertical container made of 316L outer material and 316L inner material sprayed with PFA stainless steel; The 100L drying kettle is a vertical container made of 316L outer material and 316L inner material sprayed with PFA stainless steel.

2. The method for operating a polypeptide cleavage system according to claim 1, characterized in that: The operating steps of the system are: A1, Inspection: Check the electrical control connection, circuit, pipe opening and pipeline sealing, equipment air tightness, measure the pressure of each gas source port, and focus on the connection and sealing between the lower cone and the cylinder of the reactor; A2, Account login: The administrator, engineer, operator and monitor login accounts are authorized respectively; A3, Startup preparation: After checking that everything is correct, press the start button in the equipment control cabinet to start the equipment; A4, Operation: After logging in to the interface, operate the system.

3. The method for operating a polypeptide cleavage system according to claim 2, characterized in that: In said A2; Administrator privileges are first-level management privileges: can log in to the highest authorized control device, can modify any instrument parameters, can control the switch and stop of pumps and valves; Engineers are the second-level permission group: they have the authority to set and modify parameters; The operator authority is a three-level authority group: only the start and stop authority of the pump and valve, and the start and stop authority of the button interlock; The supervisor authority is the driver authority group: only has the supervisor authority.

4. The method for operating a polypeptide cleavage system according to claim 2, characterized in that: The startup preparation in A3 is divided into: B1, turn on the power supply; turn on the nitrogen and compressed air sources; B2, check the control cabinet; check the aging of the power supply and components of the control cabinet; B3, Check the instrument: Check the instrument collection values ​​and system parameters; B4, check the solenoid valve: check the solenoid valves and pumps for stuck phenomenon.

5. The method for operating a polypeptide cleavage system according to claim 2, characterized in that: The operations in A4 are divided into: interface login, process operation, parameter setting, alarm record, user management, system information query and system setting.

6. The method for operating a polypeptide cleavage system according to claim 5, characterized in that: The process operation is divided into: cleaning, MTBE liquid feeding, TFA feeding, cracking kettle discharging, liquid drainage, settling kettle liquid transfer, and discharging.

7. The method for operating a polypeptide cleavage system according to claim 6, characterized in that: The cleaning steps are: click the cleaning button, select the cleaning amount and the nitrogen purge seconds, and click the kettle body to be cleaned; The steps for MTBE and liquid injection are: click the liquid injection button, select the liquid injection volume and purge seconds, and select the kettle body to be injected; The steps of TFA feeding are: ① click the liquid feeding button; select the liquid feeding volume and purge seconds; Click to start the liquid injection, and click again to stop the liquid injection; The steps for discharging the cracking kettle are: click on the pop-up window of discharging the cracking kettle, select the container to be transferred into, and the corresponding valve will open automatically after clicking, and it will stop after clicking again.

8. The method for operating a polypeptide cleavage system according to claim 6, characterized in that: The step of draining liquid is to click on the pop-up window of the three-in-one waste liquid discharge to select start, and the solution in the drying kettle will be discharged, and click on the stop button to stop draining liquid; The steps of liquid transfer in the settling kettle are: click the bottom valve of the settling kettle, select start, pressurize the nitrogen in the settling kettle, and the solution in the settling kettle will be transferred into the three-in-one kettle through pressure transfer; The steps of discharging are as follows: click the bottom valve at the lower end of the kettle body, and after starting, the eccentric butterfly valve at the bottom of the kettle body opens, and the solid material will fall naturally with gravity.

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