Wulian Yijintong for relieving ischemic stroke and preparation method of Wulian Yijintong

Abstract

The invention discloses a Wulian Yijintong for relieving ischemic stroke. The Wulian Yijintong comprises euonymus fortunei, polygala fallax hemsl, caulis spatholobi, tinospora sinensis merr, giantleaf ardisia rhizome, beautiful millettia root, radix fici simplicissimae and pseudo-ginseng. Compared with the prior art, according to etiological analysis and treatment mechanism of ischemic stroke based on the Zhuang medicine theory, tinospora sinensis merr for dispelling wind, polygala fallax hemsl for reinforcing qi and nourishing blood, and caulis spatholobi for promoting blood circulation and enriching blood are combined as main drugs to jointly achieve the effects of dispelling wind and removing toxicity, regulating qi activity, promoting synchronization of three qi and dredging two paths and three paths through airborne blood circulation; the content of ferrous ions in blood and the water content of the brain are reduced, the content of glutathione is increased, and the cerebral infarction rate is reduced.

Description

Technical Field

[0001] The invention relates to the field of traditional Chinese medicines, and in particular to a Wulian Yinaotong for alleviating ischemic stroke and a preparation method thereof. Background Art

[0002] Ischemic stroke is caused by insufficient blood supply to a certain area of ​​the brain due to vascular embolism. Insufficient blood supply causes ischemic damage or necrosis of brain tissue, leading to irreversible neurological deficits. Ischemic stroke can cause varying degrees and types of brain damage, including brain tissue lesions and neuronal death.

[0003] Since ischemic stroke may be related to coronary atherosclerosis, cardiogenic factors, arteriolar occlusion and other factors, patients often have symptoms such as limb numbness, dizziness, headache, and may also have symptoms such as language disorders and dysphagia. It is the second leading cause of death in the world and one of the diseases with a very high disability rate. Moreover, once an ischemic stroke occurs, the chance of recurrence is high, and long-term medication is usually required to control the disease and prevent recurrence. Because ischemic stroke is often accompanied by hypertension, hyperlipidemia and other diseases, if Western medicine is used to prevent and treat patients, they need to take multiple types of drugs at the same time, which may increase the burden on the patient's internal organs. Summary of the invention

[0004] The purpose of the present application is to provide a Wulian Yinaotong for alleviating ischemic stroke.

[0005] The purpose of the present application is also to provide a preparation method of Wulian Yinaotong for alleviating ischemic stroke.

[0006] To achieve the above objectives, the present application provides a Wulian Yinaotong for alleviating ischemic group, wherein the Wulian Yinaotong includes Euonymus fortunei, Herba Lycopodii flavescentis, Caulis Spatholobi, Herba Lycopodii flavescentis, Herba Zoumae, Herba Cynanchifoliae, Herba Polygoni Multiflori and Panax notoginseng.

[0007] Preferably, in the above technical scheme, the raw materials of the Wulian Yinaotong include, by weight, 10-20g of Euonymus fortunei, 10-20g of Prunus mume, 10-20g of Millettia reticulata, 5-15g of Rhizoma Cynoglossi, 10-20g of Caulis Spatholobi, 5-15g of Rhizoma Cynoglossi, 10-20g of Caulis Spatholobi, 5-15g of Trametes australis, 10-20g of Polygonum multiflorum and 5-15g of Panax notoginseng.

[0008] Preferably, in the above technical scheme, the raw materials of the Wulian Yinaotong include, by weight, 13-17g of Euonymus fortunei, 13-17g of Prunus mume, 13-17g of Millettia reticulata, 8-12g of Rhizoma Cynoglossi, 13-17g of Caulis Spatholobi, 8-12g of Radix Codonopsis pilosulae, 13-17g of Rhizoma Cynoglossi, 8-12g of Trametes australis, 13-17g of Polygonum multiflorum and 8-10g of Panax notoginseng.

[0009] Preferably, in the above technical scheme, the raw materials of the Wulian Yinaotong include, by weight, 15g of Caulis dahuricae, 15g of Elaeagnus chinensis, 15g of Millettia reticulata, 10g of Rhizoma Cynoglossi, 15g of Caulis Achyranthis Bidentatae, 10g of Radix Eupatorii, 15g of Radix Eupatorii, 15g of Radix Eupatoriae and 9g of Radix Notoginseng.

[0010] Preferably, in the above technical solution, the Wulian Yinaotong also includes Prunella Vulgaris and Pheretima.

[0011] Preferably, in the above technical scheme, the raw materials of the Wulian Yinaotong include, by weight, 10-20g of Euonymus fortunei, 10-20g of Prunella Vulgaris, 10-20g of Millettia reticulata, 5-15g of Rhizoma Cynoglossi, 10-20g of Caulis Trichosanthis, 5-15g of Radix Achyranthis Bidentatae, 10-20g of Rhizoma Cynoglossi, 5-15g of Trametes dasyphyllae, 10-20g of Herba Polygoni Multiflori, 5-15g of Panax notoginseng, 5-15g of Pheretima and 5-15g of Herba Prunellae.

[0012] Preferably, in the above technical scheme, the raw materials of the Wulian Yinaotong include, by weight, 15g of Euonymus fortunei, 15g of Eleutherodactyla quinquefolia, 15g of Millettia spatholobi, 10g of Rhizoma Cynoglossi, 15g of Caulis Achyranthis Bidentatae, 10g of Radix Achyranthis Bidentatae, 15g of Herba Lycopodii, 9g of Panax notoginseng, 10g of Pheretima chinensis and 10g of Herba Prunellae.

[0013] A preparation method of Wulian Yinaotong for alleviating ischemic group, the preparation method comprising:

[0014] S1, cut Millettia spatholobi, Euonymus fortunei, Eleutherodactyla quinquefolia, Cynanchum indica, and Cynanchum chinense into small pieces, grind Panax notoginseng into powder, and weigh out Cynanchum fortunei, Eleutherodactyla quinquefolia, Millettia spatholobi, Cynanchum indica, Cynanchum chinense ... and Panax notoginseng powder;

[0015] S2, soak Euonymus fortunei, Eleutherodactyla quinquefolia, Millettia spatholobi, Rhizoma Cynoglossi, Rhizoma Achyranthis Bidentatae, and Radix Zoumae in 500-800mL of water for 20-40min, boil over high heat, simmer over low heat for 10-20min, add Polygonum multiflorum and Panax notoginseng powder, simmer over low heat for 10-20min, filter, take the medicinal liquid, and prepare Wulian Yinaotong.

[0016] Preferably, in the above technical scheme, the part of the medicinal materials includes five or more of the following: Eucommia fortunei, Elsholtzia quinata, Millettia spatholobi, Rhizoma Cynoglossi, Caulis ternatae, Caulis lappai, Fenugreek root, Pheretima chinensis and Herba Prunellae.

[0017] Preferably, in the above technical solution, the Wulian Yinaotong is an oral medicine, and the method of taking it is to take it warm once in the morning, noon and evening.

[0018] The medicinal properties and efficacy of the medicinal materials used in the present invention are as follows:

[0019] Euonymus fortunei (Turcz.) Hand.-Mazz is an evergreen vine shrub of the genus Euonymus in the family Celastraceae. It is also known as golden thread wind, nine-cow-making, wall-keeping wind, vine, and wall-climbing grass. It is bitter and sweet, slightly spicy and slightly warm. It has the effects of enhancing the body's immune function, strengthening the heart, stopping bleeding, preventing the formation of blood clots, relaxing muscles and tendons, and promoting qi and blood circulation. It is used to treat waist and knee pain, hemiplegia, and rheumatic pain.

[0020] Five-fingered ginseng: (scientific name Ficus simplicissima Lour.), also known as five-fingered milk, five-fingered incense, five-fingered banyan, five-clawed dragon, sow milk, is the dried root of the plant of the genus Ficus in the Moraceae family. It tastes sweet and is neutral in nature; it enters the lung, spleen, and liver meridians. Five-fingered ginseng has the effects of strengthening the spleen and removing dampness, promoting blood circulation and removing blood stasis, dispelling cold and relieving pain. It is mainly used to treat symptoms such as spleen deficiency edema, poor appetite and weakness, cough due to tuberculosis, night sweats, leucorrhea, postpartum agalactia, rheumatic pain, edema, ascites due to cirrhosis, hepatitis, and traumatic injuries.

[0021] Spatholobus suberectus Dunn: (scientific name Spatholobus suberectus Dunn), also known as blood wind vine, blood wind, blood vine, three-leaf Spatholobus, nine-layer wind, is the vine stem of leguminous plants such as dense flower bean, white flower oil vine, fragrant flower rock bean vine or bright leaf rock bean vine. It tastes bitter and sweet, and is warm in nature; it enters the liver and kidney meridians. Spatholobus suberectus has the effects of promoting blood circulation, replenishing blood, relaxing muscles and tendons, regulating menstruation and relieving pain. It is mainly used to treat low back and knee pain, numbness and paralysis, rheumatism, blood deficiency and chlorosis, irregular menstruation and other symptoms.

[0022] Millettia speciosa Champ., also known as beautiful millettia, mountain lotus root, and powerful potato. It tastes sweet and is neutral in nature. It enters the lung and kidney meridians. Millettia speciosa Champ. has the effects of nourishing the lungs, strengthening the tendons and activating the collaterals. It is mainly used to treat lumbar muscle strain, rheumatoid arthritis, lung heat, lung deficiency cough, tuberculosis, chronic bronchitis, chronic hepatitis, spermatorrhea and other diseases.

[0023] Kuanjinteng: Also known as Songgenteng, Shujinteng, Dasongshen, it is the stem of the Chinese blue ox gall of the Menispermaceae family. It tastes bitter and is cool in nature; it enters the liver meridian. Kuanjinteng has the effects of relaxing muscles and tendons, dispelling wind and relieving pain. It is mainly used to treat rheumatic pain, sciatica, lumbar muscle strain, traumatic injuries and other diseases.

[0024] Radix Ardisiae Gigantifoliae, also known as Radix Ardisiae Gigantifoliae, is the dried root of the plant Radix Ardisiae of the Myrsinaceae family. It is pungent and bitter in taste and warm in nature. It enters the lung, liver and stomach meridians. It has the effects of dispelling wind and activating blood circulation, strengthening tendons and bones, dispersing blood stasis and reducing swelling, detoxifying and relieving pain. It is mainly used to treat body pain caused by colds, headaches, and rheumatic joint and tendon pain.

[0025] Polygala fallax Hemsl. is the dried root of Polygala fallax Hemsl. of the Polygalaceae family. It is sweet and neutral in nature. It enters the liver, kidney and spleen meridians. Polygala fallax Hemsl. has the effects of nourishing qi and blood, strengthening the spleen and removing dampness, activating blood circulation and regulating menstruation, nourishing and strengthening, removing dampness and dispersing blood stasis, and nourishing qi and blood. It is mainly used to treat lumbar disc herniation, anti-fatigue, and coronary artery resistance affecting myocardial function.

[0026] Panax notoginseng: (scientific name Panax notoginseng (Burk.) FHChen) is the dried root of the Araliaceae plant Panax notoginseng, also known as Tianqi and Jinbuhuan. It tastes sweet and slightly bitter, and is warm in nature. It enters the liver and stomach meridians. Panax notoginseng has the effects of promoting blood circulation and removing blood stasis, improving cardiovascular diseases, nourishing blood, enhancing immunity and relieving pain. It is mainly used to treat coronary heart disease, hypertension, migraine, chronic atrophic gastritis, acute cardiovascular and cerebrovascular diseases, etc.

[0027] Prunella vulgaris: (scientific name: Prunella vulgaris), is a plant of the genus Prunella in the family Lamiaceae. It is also known as Jiuchonglou, Tiesecao, Datouhua, Bangshenhua, and Yangchangcai. It tastes bitter and pungent, and is cold in nature. It enters the liver and gallbladder meridians. Prunella vulgaris has the effects of clearing the liver and improving eyesight, dispersing stagnation and reducing swelling, clearing away heat and detoxifying, and lowering blood pressure. It is mainly used to treat symptoms such as red and swollen eyes, headaches and dizziness, and night pain in the eyes.

[0028] Earthworm: (scientific name: Pheretima) is the dried whole body of the giant earthworm, Pheretima radiata or Pheretima radiata. It tastes salty and is cold in nature. It enters the liver, spleen and bladder meridians. Earthworm has the effects of dispelling wind, clearing heat, calming spasms, unblocking meridians, relieving asthma and diuresis. It is mainly used to treat rheumatic pain, hemiplegia, heat-induced convulsions and other diseases.

[0029] Compared with the prior art, this application has the following beneficial effects:

[0030] (1) This prescription is based on the analysis of the etiology of ischemic stroke in Zhuang medicine theory: the three qi are not synchronized, the airway is blocked, causing qi deficiency and fatigue, qi stagnation and blood deficiency lead to poor blood circulation, the three channels and two roads are blocked, the three channels and dragon roads are blocked, the nutrition delivery of the internal organs, bones and flesh is blocked, the internal organs are deficient and weak, the qi is weak, the blood flow is slow, and the vascular embolism is blocked, and the three channels and dragon roads affect the nerve information transmission and are blocked; if the external evil invades occasionally, it will cause the qi and blood to be disordered, and the blood rushes to the brain, which is easy to cause stroke and fainting. The treatment mechanism is: dispel wind, replenish qi, regulate qi, promote qi movement, regulate qi, coordinate blood nourishment, activate blood circulation, promote smooth blood circulation, so as to achieve the effect of unblocking the three channels and two roads, harmonizing qi and blood, balancing yin and yang, and synchronizing the three qi.

[0031] (2) The formula of this group is explained as: Kuanjinteng dispels wind, Huanghuadaoshuilian replenishes qi and nourishes blood, and Jixueteng promotes blood circulation and replenishes blood as the main medicine, dispels wind and removes toxins, regulates qi, promotes the synchronization of the three qi, and qi carries blood to dredge the two routes and three channels; Zoumatai promotes blood circulation, Niu Dali replenishes qi and nourishes blood, Sanqi replenishes blood and nourishes blood, and Fufangteng promotes qi and blood circulation as the auxiliary medicine, which strengthens the main medicine qi carries blood circulation and promotes the circulation of qi and blood to bones and flesh, and Wuzhimaotao leads all the medicines directly to the diseased area and regulates the two routes, thus becoming the supporting medicine. The eight medicines work together to regulate qi and blood, unblock the three routes and two channels, and regulate the synchronization of the three qi. BRIEF DESCRIPTION OF THE DRAWINGS

[0032] The realization of the purpose, functional features and advantages of the present invention will be further explained in conjunction with embodiments and with reference to the accompanying drawings.

[0033] Figure 1 is a graph of the standard curve for ferrous ion;

[0034] Figure 2 is a graph of the glutathione standard curve;

[0035] Figure 3 It is a graph of TFR expression in WB experiments and its internal reference;

[0036] Figure 4 is a graph of PTGS2 expression in WB experiments and its internal reference;

[0037] Figure 5 is a graph showing GPX4 expression in WB experiments and its internal reference;

[0038] Figure 6 It is a TTC staining diagram of the cerebral infarction volume ratio of the sham operation group, the model group, Example 1 and Comparative Examples 1-3;

[0039] Figure 7 It is a TTC staining diagram of the cerebral infarction volume ratio of the sham operation group, the model group, Example 6 and Comparative Example 4;

[0040] Figure 8 This is a picture of HE staining of brain tissue of a rat in the sham operation group;

[0041] Fig. 9 This is a picture of HE staining of brain tissue of a rat in the model group;

[0042] Fig.10 is a picture of HE staining of a rat brain tissue in Example 1;

[0043] Fig.11 is a picture of HE staining of a rat brain tissue in Example 6;

[0044] Fig.12 This is a picture of HE staining of a rat brain tissue in Comparative Example 1;

[0045] Fig.13 This is a picture of HE staining of a rat brain tissue in Comparative Example 2;

[0046] Fig.14 This is a picture of HE staining of a rat brain tissue in Comparative Example 3;

[0047] Fig.15 This is a picture of HE staining of a rat brain tissue in Comparative Example 4. DETAILED DESCRIPTION

[0048] The technical solutions in the embodiments of the present invention are described clearly and completely. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0049] Example 1

[0050] A Wulian Yinaotong for relieving ischemic stroke. The raw materials of the Wulian Yinaotong are made from 15g of Euonymus fortunei, 15g of Prunus mume, 15g of Millettia spatholobi, 10g of Rhizoma Cynoglossi, 15g of Caulis Achyranthis Bidentatae, 10g of Caulis Trichosanthis, 10g of Fenugreek, 15g of Polygonum multiflorum and 9g of Panax notoginseng by weight.

[0051] A preparation method of Wulian Yinaotong for alleviating ischemic group, the preparation method comprising:

[0052] S1, cut Millettia spatholobi, Euonymus fortunei, Eleutherodactyla quinquefolia, Rhizoma Cynoglossi, and Rhizoma Cynoglossi into small pieces, grind Panax notoginseng into powder, and weigh the medicinal materials;

[0053] S2. Soak Euonymus fortunei, Eleutherodactyla quinquefolia, Millettia spatholobi, Rhizoma Cynoglossi, Rhizoma Cynoglossi and Radix Eupatorii in 800 mL of water for 40 min, boil over high heat, simmer over low heat for 20 min, add Polygonum multiflorum and Panax notoginseng powder, simmer over low heat for 15 min, filter, take the medicinal liquid, and prepare Wulian Yinaotong.

[0054] Example 2

[0055] A Wulian Yinaotong for relieving ischemic stroke. The raw materials of the Wulian Yinaotong are made of 17g of Euonymus fortunei, 17g of Prunus mume, 17g of Millettia spatholobi, 12g of Rhizoma Cynoglossi, 17g of Caulis Achyranthis Bidentatae, 12g of Caulis Trichosanthis, 12g of Trametes australis, 17g of Polygonum multiflorum and 10g of Panax notoginseng by weight.

[0056] A preparation method of Wulian Yinaotong for alleviating ischemic group, the preparation method comprising:

[0057] S1, cut Millettia spatholobi, Euonymus fortunei, Eleutherodactyla quinquefolia, Rhizoma Cynoglossi, and Rhizoma Cynoglossi into small pieces, grind Panax notoginseng into powder, and weigh the medicinal materials;

[0058] S2. Soak Euonymus fortunei, Eleutherodactyla quinquefolia, Millettia spatholobi, Cynanchum wilfordii, Caulis lappa and Trametes australis in 800 mL of water for 40 min, boil over high heat, simmer over low heat for 20 min, add Polygonum multiflorum and Panax notoginseng powder, simmer over low heat for 20 min, filter, take the medicinal liquid, and prepare Wulian Yinaotong.

[0059] Example 3

[0060] A Wulian Yinaotong for relieving ischemic stroke. The raw materials of the Wulian Yinaotong are made of 13g of Euonymus fortunei, 13g of Prunus mume, 13g of Millettia spatholobi, 8g of Rhizoma Cynoglossi, 13g of Caulis Achyranthis Bidentatae, 8g of Caulis Trichosanthis, 8g of Radix Achyranthis Bidentatae, 13g of Radix Eupatorii Flavescentis and 8g of Radix Notoginseng by weight.

[0061] A preparation method of Wulian Yinaotong for alleviating ischemic group, the preparation method comprising:

[0062] S1, cut Millettia spatholobi, Euonymus fortunei, Eleutherodactyla quinquefolia, Rhizoma Cynoglossi, and Rhizoma Cynoglossi into small pieces, grind Panax notoginseng into powder, and weigh the medicinal materials;

[0063] S2. Soak Euonymus fortunei, Eleutherodactyla quinquefolia, Millettia spatholobi, Rhizoma Cynoglossi, Rhizoma Cynoglossi and Radix Eupatorii in 500 mL of water for 30 min, boil over high heat, simmer over low heat for 15 min, add Polygonum multiflorum and Radix notoginseng powder, simmer over low heat for 15 min, filter, take the medicinal liquid, and prepare Wulian Yinaotong.

[0064] Example 4

[0065] A Wulian Yinaotong for relieving ischemic stroke. The raw materials of the Wulian Yinaotong are made of 20g of Euonymus fortunei, 20g of Prunus mume, 20g of Millettia reticulata, 15g of Rhizoma Cynoglossi, 20g of Caulis Spatholobi, 15g of Rhizoma Cynoglossi, 15g of Caulis Achyranthis Bidentatae, 15g of Trametes acutissima, 20g of Polygonum multiflorum and 15g of Panax notoginseng by weight.

[0066] A preparation method of Wulian Yinaotong for alleviating ischemic group, the preparation method comprising:

[0067] S1, cut Millettia spatholobi, Euonymus fortunei, Eleutherodactyla quinquefolia, Rhizoma Cynoglossi, and Rhizoma Cynoglossi into small pieces, grind Panax notoginseng into powder, and weigh the medicinal materials;

[0068] S2. Soak Euonymus fortunei, Eleutherodactyla quinquefolia, Millettia spatholobi, Cynanchum wilfordii, Caulis lappa and Trametes australis in 800 mL of water for 40 min, boil over high heat, simmer over low heat for 20 min, add Polygonum multiflorum and Panax notoginseng powder, simmer over low heat for 20 min, filter, take the medicinal liquid, and prepare Wulian Yinaotong.

[0069] Example 5

[0070] A Wulian Yinaotong for relieving ischemic stroke. The raw materials of the Wulian Yinaotong are made of 10g of Euonymus fortunei, 10g of Prunus mume, 10g of Millettia reticulata, 5g of Rhizoma Cynoglossi, 10g of Caulis Achyranthis Bidentatae, 5g of Caulis Spatholobi, 5g of Caulis Achyranthis Bidentatae, 10g of Caulis Spatholobi and 5g of Panax notoginseng by weight.

[0071] A preparation method of Wulian Yinaotong for alleviating ischemic group, the preparation method comprising:

[0072] S1, cut Millettia spatholobi, Euonymus fortunei, Eleutherodactyla quinquefolia, Rhizoma Cynoglossi, and Rhizoma Cynoglossi into small pieces, grind Panax notoginseng into powder, and weigh the medicinal materials;

[0073] S2. Soak Euonymus fortunei, Eleutherodactyla quinquefolia, Millettia spatholobi, Rhizoma Cynoglossi, Rhizoma Cynoglossi and Radix Eupatorii in 500 mL of water for 20 min, boil over high heat, simmer over low heat for 10 min, add Polygonum multiflorum and Panax notoginseng powder, simmer over low heat for 10 min, filter, take the medicinal liquid, and prepare Wulian Yinaotong.

[0074] Example 6

[0075] A Wulian Yinaotong for relieving ischemic stroke. The raw materials of the Wulian Yinaotong are made from 15g of Euonymus fortunei, 15g of Prunella vulgaris, 15g of Millettia spatholobi, 10g of Rhizoma Cynoglossi, 15g of Caulis Achyranthis Bidentatae, 10g of Fenugreek root, 15g of Polygonum multiflorum, 10g of Trametes acutissima, 15g of Polygonum multiflorum, 9g of Panax notoginseng, 10g of Pheretima and 10g of Herba Prunellae.

[0076] A preparation method of Wulian Yinaotong for alleviating ischemic group, the preparation method comprising:

[0077] S1, cut Millettia spatholobi, Euonymus fortunei, Eleutherodactyla quinquefolia, Rhizoma Cynoglossi, and Rhizoma Cynoglossi into small pieces, grind Panax notoginseng into powder, and weigh the medicinal materials;

[0078] S2, soak Euonymus fortunei, Eleutherodactyla quinquefolia, Millettia spatholobi, Rhizoma Cynanchum, Rhizoma Cynanchum, Pheretima ternata, and Herba Prunellae in 600 mL of water for 20 min, boil over high heat, simmer over low heat for 10 min, add Polygonum multiflorum and Panax notoginseng powder, simmer over low heat for 10 min, filter, take the medicinal liquid, and prepare Wulian Yinaotong.

[0079] Comparative Example 1

[0080] It is basically the same as Example 1, except that the components are different.

[0081] A Wulian Yinaotong for relieving ischemic stroke. The raw materials of the Wulian Yinaotong are made from 15g of Euonymus fortunei, 15g of Prunus mume, 15g of Acorus calamus, 10g of Rhizoma Cynoglossi, 15g of Rhizoma Cynoglossi, 10g of Radix Codonopsis pilosulae, 15g of Rhizoma Cynoglossi, 10g of Radix Eupatorii, 15g of Rhizoma Cynoglossi and 9g of Radix Notoginseng by weight.

[0082] Comparative Example 2

[0083] It is basically the same as Example 1, except that the components are different.

[0084] A Wulian Yinaotong for relieving ischemic stroke. The raw materials of the Wulian Yinaotong are made from 15g of Euonymus fortunei, 15g of Prunus mume, 15g of Acorus calamus, 10g of Rhizoma Cynomorii, 10g of Fenugreek, 15g of Polygonum multiflorum and 9g of Panax notoginseng by weight.

[0085] Comparative Example 3

[0086] It is basically the same as Example 1, except that the components are different.

[0087] A Wulian Yinaotong for relieving ischemic stroke. The raw materials of the Wulian Yinaotong are made from 15g of Euonymus fortunei, 15g of Prunus armeniaca, 10g of Trametes australis, 15g of Polygonum multiflorum and 9g of Panax notoginseng by weight.

[0088] Comparative Example 4

[0089] It is basically the same as Example 6, except that the components are different.

[0090] A Wulian Yinaotong for relieving ischemic stroke. The raw materials of the Wulian Yinaotong are made from 15g of Euonymus fortunei, 15g of Millettia spatholobi, 10g of Radix Achyranthis Bidentatae, 10g of Trametes australis, 15g of Polygonum multiflorum, 9g of Panax notoginseng and 10g of Pheretima lumbricoides by weight.

[0091] 1. Experimental subjects:

[0092] The experimental animals were SPF male SD rats, 6-8 weeks old, weighing 180-220 g, provided by the laboratory of Guangxi University of Chinese Medicine.

[0093] 2. Build the model:

[0094] SD rats were subjected to middle cerebral artery occlusion to simulate ischemic stroke, specifically: rats were subjected to a longitudinal incision in the middle of the neck, skin and fascia were separated, deep neck membrane was torn, blunt separation was performed downward along the left edge of the sternocleidomastoid muscle, the left common carotid artery, the internal carotid artery and the external carotid artery were exposed, a small cut was made on the external carotid artery, the prepared embolism line was inserted from the external neck incision, turned into the internal carotid artery, and the line plug was slowly pushed into the middle cerebral artery at 45° obliquely upward to block the middle cerebral artery opening. After two hours of embolism, the head of the embolism line was gently withdrawn to the common carotid artery, and the redundant embolism line was cut off to cause ischemia-reperfusion injury. The model group and the sham operation group were gavaged with normal saline, and the corresponding Wulian Yinaotong was gavaged with Example 1, Example 6 and Comparative Examples 1-4.

[0095] 3. The experimental rats were subjected to behavioral assessment (as shown in Tables 1 and 2), and the assessment results were used for the motor ability of the rats. The behavioral assessment used the Zea-Longa scoring method to evaluate the degree of neurological impairment in the rats. The scoring criteria of the Zea-Longa scoring method are as follows:

[0096] 0 points: no neurological deficit;

[0097] 1 point: when the tail is lifted, the front paw on the paralyzed side cannot be fully extended but retracts inward;

[0098] 2 points: turning to the opposite side during spontaneous walking;

[0099] 3 points: leaning to the opposite side during spontaneous walking and being unable to stand;

[0100] 4 points means the patient cannot walk spontaneously and has loss of consciousness.

[0101] Table 1 Behavioral scores of the model group, sham operation group, Example 1 and Example 6

[0102]

[0103] Table 2 Behavioral scores of comparative examples 1-4

[0104]

[0105] The results are shown in Tables 1 and 2. The lowest score of the sham operation group in each period was 0, the behavioral score of the model group was the highest, and the behavioral scores of Example 1, Example 6 and Comparative Examples 1-4 were lower than those of the model group, indicating that the behavioral scores of the rats in the model group would increase if the model group did not administer the drug, indicating that the agent of the present application can effectively reduce the behavioral scores of the model rats to a certain extent.

[0106] 4. The concentrations of ferrous ion solutions used to draw the ferrous ion standard curve were 0 ug / mL, 2 ug / mL, 4 ug / mL, 6 ug / mL, 8 ug / mL, and 10 ug / mL. The OD values ​​of the above ferrous ions were determined by colorimetry. The results are shown in Table 3.

[0107] Table 3 OD values ​​for drawing the standard curve of ferrous ions

[0108]

[0109] Draw a standard curve based on the above solution concentrations and the corresponding mean values. Draw the obtained standard curve as shown in Figure 1 As shown, the standard curve equation of ferrous ion is Y = 15.521x-0.6729, R 2 =0.9991.

[0110] The OD values ​​of the sham operation group, model group, Example 1, Example 6, Comparative Example 1, Comparative Example 2, Comparative Example 3 and Comparative Example 4 were measured by colorimetry, the OD mean was calculated, and the OD mean was substituted into the formula Y=15.521x-0.6729 to obtain the corresponding concentration. The results are shown in Table 4.

[0111] Table 4 OD mean and ferrous ion concentration of experimental rats in each group

[0112] OD mean Concentration (ug / mL) Concentration (ug / g) Sham operation group 0.186 2.212 22.12 Model Group 0.247 3.159 31.59 Example 1 0.210 2.585 25.85 Example 6 0.195 2.354 23.54 Comparative Example 1 0.220 2.742 27.42 Comparative Example 2 0.227 2.850 28.50 Comparative Example 3 0.240 3.052 30.52 Comparative Example 4 0.226 2.835 28.35

[0113] As can be seen from Table 4, the ferrous ion concentration of the model group is 31.59ug / g, which is much higher than the ferrous ion concentration of the sham operation. After administration, the ferrous ion concentration of the ischemic stroke model rats of Example 1, Example 6 and Comparative Examples 1-4 decreased to varying degrees relative to the rats in the model group, among which the ferrous ion concentration of Example 1 and Example 6 decreased the most, indicating that the decoction of Wulian Yinaotong of the present application has a significant therapeutic effect on reducing the ferrous ions in the blood of ischemic stroke rats.

[0114] 5. The concentrations of the glutathione solution gradient used to draw the glutathione standard curve were 0 ug / mL, 25 ug / mL, 50 ug / mL, 100 ug / mL, 200 ug / mL, and 300 ug / mL. The OD values ​​of the above glutathione were determined by colorimetry. The results are shown in Table 5.

[0115] Table 5 Data used to draw the glutathione standard curve

[0116]

[0117] Draw a standard curve based on the above solution concentrations and the corresponding mean values. Draw the obtained standard curve as shown in Figure 2 As shown, the glutathione standard curve equation is Y = 424.44x-46.241, R 2 =0.9996.

[0118] The glutathione OD values ​​of the sham operation group, model group, Example 1, Example 6, Comparative Example 1, Comparative Example 2, Comparative Example 3 and Comparative Example 4 were respectively determined by colorimetry, the OD mean was calculated, and the OD mean was substituted into the formula Y=424.44x-46.241 to obtain the corresponding concentration. The results are shown in Table 6.

[0119] Table 6 OD mean and glutathione content of experimental rats in each group

[0120]

[0121]

[0122] It can be concluded from Table 6 that the glutathione content of rats in the model group is relatively low. The low glutathione content generally reflects the reduced detoxification ability or antioxidant capacity of the liver in the body. The use of Wulian Yinaotong in Example 1 and Example 6 can increase the glutathione content to be close to the glutathione content in the sham operation group, indicating that the Wulian Yinaotong of the present application has the ability to improve the detoxification ability of the liver or enhance the antioxidant capacity of the body.

[0123] 6. In each group, the brains of 3 ischemic stroke model rats were taken to measure their dry weight and wet weight, and the brain water content (%) was calculated. The mean dry weight, mean wet weight and water content are shown in Table 7. The calculation formula of the water content is as follows: Water content (%) = (wet weight-dry weight) / dry weight.

[0124] Table 7 Brain water content in each group

[0125] Average dry weight (g) Average wet weight (g) Water content (%) Sham operation group 0.505 1.796 71.89 Model Group 0.338 1.940 82.59 Example 1 0.470 1.847 74.57 Example 6 0.484 1.827 73.52 Comparative Example 1 0.435 1.869 76.70 Comparative Example 2 0.424 1.879 77.43 Comparative Example 3 0.348 1.895 81.64 Comparative Example 4 0.465 1.870 75.15

[0126] From Table 7, it can be concluded that ischemic stroke causes local edema, which leads to an increase in brain wet weight and brain water content. The brain wet weight of ischemic stroke rats treated with Wulian Yinaotong is still higher than that of the sham operation group, but significantly lower than that of the model group, indicating that taking Wulian Yinaotong can alleviate local edema caused by ischemic stroke and reduce the expansion of brain nerve and tissue damage or death caused by local edema.

[0127] 6. The expression of TFR (transferrin receptor), GAPDH (glyceraldehyde-3-phosphate dehydrogenase), PTGS2 (cyclooxygenase 2), and GPX4 (glutathione peroxidase 4) in the sham operation group, model group, and Example 1 was detected. The expression of GAPDH was analyzed twice, and the expression results are shown in Tables 8 and 9. The expression of TFR is shown in Tables 8 and 9. Figure 3 As shown, PTGS2 expression Figure 4 As shown, GPX4 expression Figure 5 shown.

[0128] Table 8 Expression of TFR, GAPDH and PTGS2 in the sham operation group and Example 1

[0129]

[0130] Table 9 Expression of TFR, GAPDH and PTGS2 in the model group and Example 1

[0131]

[0132]

[0133] 7. The cerebral infarction volume of the model rats in the model group and the Example 1 group was measured by TTC staining, and photos of the infarcted lesions of the stained brain tissue were taken. The normal tissue was red, and the infarcted area was grayish white. Figure 6 and Figure 7 ) The images were analyzed and processed using Image-Pro Plus 6.0 software to calculate the percentage of brain tissue infarction volume in the whole brain. The results are shown in Table 10.

[0134] Table 10 Ratio of cerebral infarction area and cerebral infarction volume in rats in the model group and Example 1

[0135]

[0136] It can be concluded from Table 0 that the Wulian Yinaotong of Example 1 using the method of the present invention significantly reduces the proportion of cerebral infarction. Comparison of Example 1 with Comparative Example 1 shows that the effect of using Millettia reticulata in Wulian Yinaotong on reducing cerebral infarction is better than that of using Acorus calamus.

[0137] 8. HE staining was used to observe the pathological changes of brain tissue in experimental rats. The specific steps were: fixation with paraformaldehyde, gradient dehydration, paraffin embedding, sectioning, baking, hematoxylin-eosin (HE) staining, and gum sealing to observe the pathological changes of brain tissue. The results were as follows: Figure 8-15 shown.

[0138] Sham operation group ( Figure 8 ),from Figure 8 It can be seen that the brain tissue structure is normal, neurons are evenly distributed, cell outlines are clear, and no obvious degeneration is observed; Fig. 9 The number of neurons in the cerebral infarction area of ​​the model group decreased, the remaining neurons were necrotic and degenerated, the cell nuclei were condensed and stained dark blue-black, the cell bodies became smaller, the surrounding tissues were loose and edematous, and the gaps were enlarged. Example 1 ( Fig.10 ) and Example 6 ( Fig.11 ) had a normal brain tissue structure and clearer cell outlines than the model group. Fig.12 ), Comparative Example 2 ( Fig.13 ), Comparative Example 3( Fig.14 ) and Comparative Example 4 ( Fig.15 ) cell bodies were also better than the model group, but not as good as Example 1 and Example 6.

[0139] The present invention can be implemented in various ways and is not limited to the embodiments described. A person skilled in the art can understand that the present invention can be implemented in other specific ways without changing the technical concept or essential features of the present invention. Therefore, it should be understood that the embodiments described above are exemplary and are not intended to limit the present invention.

Claims

1. A Wulian Yinaotong for alleviating ischemic stroke, characterized in that: The Wulian Yinaotong herb includes Euphorbia fargesii, Euphorbia glutinosae, Millettia reticulata, Caulis ternata, Caulis ternata, Caulis dasyphyllae, Psoralea corylifolia and Panax notoginseng.

2. The Wulian Yinaotong for alleviating ischemic stroke according to claim 1, characterized in that: By weight, the raw materials of the Wulian Yinaotong include 10-20g of Euonymus fortunei, 10-20g of Prunus mume, 10-20g of Millettia reticulata, 5-15g of Rhizoma Cynoglossi, 10-20g of Caulis Trichosanthis, 5-15g of Radix Achyranthis Bidentatae, 10-20g of Rhizoma Cynoglossi, 5-15g of Trametes australis, 10-20g of Polygonum multiflorum and 5-15g of Panax notoginseng.

3. The Wulian Yinaotong for alleviating ischemic stroke as claimed in claim 2, characterized in that: By weight, the raw materials of the Wulian Yinaotong include 13-17g of Euonymus fortunei, 13-17g of Prunus mume, 13-17g of Millettia reticulata, 8-12g of Rhizoma Cynoglossi, 13-17g of Caulis Spatholobi, 8-12g of Rhizoma Cynoglossi, 13-17g of Caulis Spatholobi, 8-12g of Trametes australis, 13-17g of Polygonum multiflorum and 8-10g of Panax notoginseng.

4. The Wulian Yinaotong for alleviating ischemic stroke as claimed in claim 3, characterized in that: By weight, the raw materials of the Wulian Yinaotong include 15g of Euonymus fortunei, 15g of Prunus mume, 15g of Millettia reticulata, 10g of Rhizoma Cynoglossi, 15g of Caulis Achyranthis Bidentatae, 10g of Radix Glehniae, 15g of Radix Eupatorii, 15g of Radix Eupatoriae and 9g of Radix Notoginseng.

5. The Wulian Yinaotong for alleviating ischemic stroke according to claim 1, characterized in that: The Wulian Yinaotong also includes Prunella Vulgaris and Pheretima.

6. The Wulian Yinaotong for alleviating ischemic stroke according to claim 1, characterized in that: By weight, the raw materials of the Wulian Yinaotong include 10-20g of Euonymus fortunei, 10-20g of Prunella vulgaris, 10-20g of Millettia reticulata, 5-15g of Rhizoma Cynoglossi, 10-20g of Caulis Trichosanthis, 5-15g of Radix Achyranthis Bidentatae, 10-20g of Rhizoma Cynoglossi, 5-15g of Radix Eupatorii, 10-20g of Herba Polygoni Multiflori, 5-15g of Radix Notoginseng, 5-15g of Pheretima and 5-15g of Herba Prunellae.

7. The Wulian Yinaotong for alleviating ischemic stroke according to claim 1, characterized in that: By weight, the raw materials of the Wulian Yinaotong include 15g of Euonymus fortunei, 15g of Eleutherodactyla quinquefolia, 15g of Millettia spatholobi, 10g of Rhizoma Cynoglossi, 15g of Caulis Achyranthis Bidentatae, 10g of Radix Eupatorii, 15g of Radix Achyranthis Bidentatae, 15g of Radix Eupatorii Flavescentis, 9g of Panax notoginseng, 10g of Pheretima and 10g of Herba Prunellae.

8. The method for preparing Wulian Yinaotong for alleviating ischemic stroke according to any one of claims 1 to 7, characterized in that: The preparation method comprises: S1, cut Millettia spatholobi, Euonymus fortunei, Eleutherodactyla quinquefolia, Rhizoma Cynoglossi, and Rhizoma Cynoglossi into small pieces, grind Panax notoginseng into powder, and weigh the medicinal materials; S2, soak some medicinal materials in 500-800mL of water for 20-40min, boil over high heat, simmer over low heat for 10-20min, add Rhizoma Coptidis and Radix Notoginseng powder, simmer over low heat for 10-20min, filter, take the medicinal liquid, and prepare Wulian Yinaotong.

9. The method for preparing Wulian Yinaotong for alleviating ischemic stroke according to claim 8, characterized in that: The described partial medicinal materials include five or more of the following: Eucommia fortunei, Eleutherococcus quinquefolius, Millettia spatholobi, Rhizoma Cynanchum, Cistanche deserticola, Cistanche deserticola, Pheretima schrenkiana, Pheretima sutchuenensis and Herba Prunellae.

10. The method for preparing Wulian Yinaotong for alleviating ischemic stroke according to claim 8, characterized in that: The Wulian Yinaotong is an internal medicine, and the method of taking it is to take it warm once in the morning, noon and evening.

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