FtACA13 gene derived from tartary buckwheat and expression vector and application of FtACA13 gene

By isolating and overexpressing the Ca2+-ATPase encoding gene FtACA13 from buckwheat, the problem of insufficient anti-salt stress performance in buckwheat was solved, and plant growth promotion and Na+ accumulation were achieved under salt stress conditions.

CN119685356AActive Publication Date: 2025-03-25INSTITUTE OF CROP SCIENCE CHINESE ACADEMY OF AGRICULTURAL SCIENCES
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Patent Information

Application Number
CN202510221141.4
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2025-02-27
Publication Date
2025-03-25
Estimated Expiration
2045-02-27

AI Technical Summary

Technical Problem

The prior art has little understanding of the genetic background and key regulatory genes of buckwheat tolerate salt and alkali, and it is difficult to effectively improve the salt stress resistance of plants.

Method used

Ca2+-ATPase and its encoding gene FtACA13 were isolated and identified from buckwheat, and the gene was overexpressed in plants by genetic engineering to improve the salt stress resistance of the plants.

Benefits of technology

By overexpressing the FtACA13 gene, the anti-salt stress performance of buckwheat is improved, the growth of plants under salt stress is promoted, and the accumulation of Na+ in the plant body is reduced.

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Abstract

The invention discloses an FtACA13 gene derived from tartary buckwheat as well as an expression vector and application of the FtACA13 gene. According to the invention, the FtACA13 gene is separated from tartary buckwheat; the polynucleotide sequence of the FACA13 gene is as shown in SEQ ID NO.1, and the amino acid sequence of the encoded protein of the FACA13 gene is as shown in SEQ ID NO.2. The FtACA13 gene is genetically transformed into buckwheat to obtain transgenic buckwheat hairy roots with the overexpressed FtACA13 gene, compared with hairy roots of a control group, the growth vigor and fresh weight of the fagopyrum tataricum hairy roots with the overexpressed FtACA13 gene under salt stress are increased, Na < + > accumulation in a plant body is remarkably low, it is proved that the FtACA13 gene can regulate and control the salt stress resistance of the plant, and the salt stress resistance of the fagopyrum tataricum hairy roots is improved. The invention has application prospects in the aspects of cultivating or breeding salt stress resistant plant varieties and the like.
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Citation Information

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