A compound pesticide containing Jinggangmycin and its application

Abstract

The present invention relates to the field of compound pesticides, and specifically to a compound pesticide containing Jinggangmycin and its application. The compound pesticide containing Jinggangmycin is composed of the following components by mass percentage: 10-70% active component, 1-3% antioxidant, 1-2% pH regulator, and the balance is a carrier and / or adjuvant; the active component includes active component A: Jinggangmycin and active component B: Danshensu, D-limonene and xanthan gum, and the mass ratio of active component A to active component B is 1: (0.4~5). The pesticide of the present invention is compounded by active component A and active component B, which can significantly improve the prevention and control effect, has a good effect in preventing and controlling rice diseases, and reduces the dosage and cost at the same time.

Description

Technical Field

[0001] The present invention relates to the technical field of compound pesticides, and in particular to a compound pesticide containing Jinggangmycin. The present invention also relates to the application of the fungicide in preventing and controlling rice sheath blight, rice blast and bacterial leaf blight. Background Art

[0002] Rice is one of the most important food crops in the world, but it is often threatened by various diseases during its growth. Rice diseases are the main factor affecting rice yield and quality. There are hundreds of rice diseases, but the more common ones are rice blast, sheath blight, leaf blight and nematode diseases. In recent years, the scope of rice diseases has continued to expand. With the increase in prevention and control inputs year by year, the annual rice loss caused by rice diseases in my country is still large.

[0003] Currently, the most commonly used pesticides for rice control are Jinggangmycin, gibberellin, carbendazim, etc. Among them, Jinggangmycin is an antibiotic produced by actinomycetes, which has strong systemicity and is easily absorbed by bacterial cells and rapidly transmitted inside them, interfering with and inhibiting the growth and development of bacterial cells. It has a significant control effect on rice sheath blight, but the efficacy is short and requires frequent use, which increases the cost of medicine and also increases drug resistance. Summary of the invention

[0004] In view of the above technical problems, the first object of the present invention is to provide a compound pesticide containing jinggangmycin, which is a compound of active component A jinggangmycin and active component B: danshensu, D-limonene and xanthan gum, which can significantly improve the prevention and control effect, has a good effect in preventing and controlling rice diseases, and at the same time reduces the dosage and cost.

[0005] The second object of the present invention is to provide a compound pesticide containing Jinggangmycin for use in preventing and controlling rice sheath blight, rice blast and bacterial leaf blight.

[0006] The first object of the present invention is achieved by the following technical solutions: A compound pesticide containing validamycin, which is composed of the following components by mass percentage: 10-70% of active components, 1-3% of antioxidants, 1-2% of pH regulators, and the balance is carriers and / or adjuvants; the active components include active component A: validamycin and active component B: danshensu, D-limonene and xanthan gum, and the mass ratio of the active component A to the active component B is 1: (0.4-5).

[0007] According to a preferred embodiment of the present invention, the mass ratio of the active component A to the active component B is 1:(0.5-2).

[0008] According to another preferred embodiment of the present invention, the mass ratio of the active component A to the active component B is 1:1.5.

[0009] According to a preferred embodiment of the present invention, the mass ratio of danshensu, D-limonene and xanthan gum in the active component B is 10:(4-6):0.05.

[0010] According to a preferred embodiment of the present invention, the compound pesticide is composed of the following components by mass percentage: 35-50% active ingredient, 3% antioxidant, 1% pH adjuster, and the remainder is carrier and / or adjuvant.

[0011] According to a preferred embodiment of the present invention, the pH value of the pesticide is 5.5-7.0.

[0012] According to a preferred embodiment of the present invention, the carrier includes but is not limited to one or more of polyglycolic acid, lactic acid-glycolic acid copolymer, polyhydroxybutyric acid, polylactide, polycaprolactone, polyorthoester or polyalkyl cyanoacrylate.

[0013] According to a preferred embodiment of the present invention, the auxiliary agent includes but is not limited to one or more of polyethylene glycol, glycerol, sodium dodecyl sulfate, sodium dodecylbenzene sulfonate, ammonium sulfate, alkylphenol polyoxyethylene ether, alkylphenol polyoxyethylene ether phosphate, fatty acid polyoxyethylene ester, benzoic acid, sodium lignin sulfonate, carboxymethyl cellulose or polyvinyl alcohol.

[0014] According to a preferred embodiment of the present invention, the antioxidant includes but is not limited to any one of vitamin E, tert-butylhydroxytoluene, propyl gallate or sulfite.

[0015] According to a preferred embodiment of the present invention, the fungicide is a wettable powder, a suspension or a water dispersible granule.

[0016] When preparing the suspension, the carrier or auxiliary agent that can be used can be a dispersant selected from sodium dodecylbenzene sulfonate, polycarboxylate or sodium lignin sulfonate; a wetting agent selected from alkylphenol polyoxyethylene ether phosphate, phenethylphenol polyoxyethylene ether phosphate, alkyl sulfate, alkyl sulfonate or naphthalene sulfonate; an organosilicon defoamer; and an adhesive selected from polyethylene glycol and glycerol.

[0017] When preparing the wettable powder of the present invention, the carrier or auxiliary agent that can be used can be a dispersant selected from polycarboxylates, lignin sulfonates or alkyl naphthalene sulfonates; a wetting agent selected from alkyl sulfonates, alkyl sulfates or naphthalene sulfonates; a carrier selected from polyglycolic acid, lactic acid-glycolic acid copolymer, polyhydroxybutyric acid, polylactide, polycaprolactone, polyorthoester or polyalkyl cyanoacrylate.

[0018] When preparing water-dispersible granules of the present invention, the carrier or auxiliary agent that can be used can be a dispersant selected from polycarboxylates, lignin sulfonates or alkyl naphthalene sulfonates; a wetting agent selected from alkyl sulfates, polyoxyethylene alcohols, alkyl sulfonates or naphthalene sulfonates; a binder selected from corn starch, microcrystalline cellulose or diatomaceous earth; a carrier selected from polyglycolic acid, lactic acid-glycolic acid copolymer, polyhydroxybutyric acid, polylactide, polycaprolactone, polyorthoester or polyalkyl cyanoacrylate.

[0019] The second object of the present invention is achieved through the following technical scheme: use of a compound pesticide containing Jinggangmycin in preventing and controlling rice sheath blight, rice blast and bacterial leaf blight.

[0020] According to a preferred embodiment of the present invention, the fungicide is applied during the rice seed treatment period or the rice growth period, and the number of applications is 1-2 times, with spraying once every 10-15 days.

[0021] According to another preferred embodiment of the present invention, the concentration of the drug solution applied each time is 300-4000 ppm.

[0022] Compared with the prior art, the present invention has the following beneficial effects: 1. In the compound pesticide containing Jinggangmycin of the present invention, the pesticide is compounded by active component A: Jinggangmycin and active component B: Danshensu, D-limonene and xanthan gum, which has a synergistic effect, can significantly improve the control effect, has a good effect in preventing and controlling rice diseases, and reduces the dosage and cost. Jinggang mold inhibits the growth and reproduction of pathogens by producing antibiotics, enzymes, etc., but the drug effect time is short, and Danshensu is a plant-derived fungicide that can prevent and control various diseases of fabrics, and can also enhance the adaptability of plants to the environment, improve productivity and disease resistance, but the effect is poor when used alone. D-limonene has the ability to inhibit the synthesis of biological macromolecules such as mycelium DNA, RNA and protein, and has a synergistic effect on Danshensu. Xanthan gum, as a stabilizer, also has a dispersing effect, which can increase the full contact between D-limonene and Danshensu and prolong the drug effect. The combination of the two active ingredients can interact through different mechanisms to reduce resistance, further improve the disease resistance of crops, increase the protection effect and duration, and thus reduce the amount of pesticides used.

[0023] 2. In the compound pesticide containing Jinggangmycin of the present invention, antioxidants are added to prevent or slow down the oxidative decomposition of the pesticide components, thereby extending the service life and improving its field stability; at the same time, pH regulators are added to control the pH value of the pesticide within the applicable range to improve safety, stability and effectiveness; the adjuvants or carriers used are all environmentally friendly substances to improve the application efficiency and reduce pollution to the environment. The components cooperate with each other, and the compound fungicide of the present invention has the advantages of high efficiency, low residue, long lasting effect and environmental friendliness.

[0024] 3. The compound pesticide containing Jinggangmycin of the present invention has a significant effect in the prevention and treatment of rice sheath blight, rice blast and bacterial leaf blight, and reduces the amount of pesticide used and prolongs the duration of efficacy, thereby reducing the cost of pesticide use. DETAILED DESCRIPTION

[0025] The technical solution of the present invention is further described in detail below in conjunction with specific implementation methods, but does not constitute any limitation to the present invention.

[0026] In the present invention, the active component A is Jinggangmycin, which is a commercially available drug; the active component B is Danshensu, D-limonene and xanthan gum. Danshensu is added to the xanthan gum aqueous solution according to the formula mass ratio of 10:5:0.05, and D-limonene is added after stirring evenly, and stirring is continued until all the ingredients are evenly mixed, insoluble impurities are filtered out, and dried at 60° C. to obtain the active component B.

[0027] Example 1 Pesticide with a mass ratio of active component A to active component B of 1:0.5 Active component A 30%, active component B 15%, antioxidant 3%, pH adjuster (phosphate buffer) 2%, sodium lignin sulfonate 2%, polyethylene glycol 3%, sodium lignin sulfonate 5%, naphthalene sulfonate 3%, and the balance is lactic acid-glycolic acid copolymer.

[0028] Add each component into the mixer according to the formula amount, stir for 20 minutes, then send the mixture into the air flow mill for ultrafine grinding to a particle size of less than 3 microns, then put it into the mixer again and stir for 30 minutes to ensure that the components are evenly distributed.

[0029] Example 2 Pesticide with a mass ratio of active component A to active component B of 1:0.75 Active component A 20%, active component B 15%, antioxidant 2%, pH adjuster 1%, sodium lignin sulfonate 2%, polyethylene glycol 3%, sodium lignin sulfonate 5%, naphthalene sulfonate 3%, and the balance is lactic acid-glycolic acid copolymer.

[0030] The preparation method is the same as that in Example 1.

[0031] Example 3 Pesticide with a mass ratio of active component A to active component B of 1:1.5 Active component A 20%, active component B 30%, antioxidant 3%, pH adjuster 2%, sodium lignin sulfonate 2%, polyethylene glycol 3%, sodium lignin sulfonate 5%, naphthalene sulfonate 3%, and the balance is lactic acid-glycolic acid copolymer.

[0032] The preparation method is the same as that in Example 1.

[0033] Example 4 Pesticide with active component A and active component B in a mass ratio of 1:2 Active component A 10%, active component B 20%, antioxidant 1%, pH adjuster 1%, sodium lignin sulfonate 2%, polyethylene glycol 3%, sodium lignin sulfonate 5%, naphthalene sulfonate 3%, and the balance is lactic acid-glycolic acid copolymer.

[0034] The preparation method is the same as that in Example 1.

[0035] Example 5 Pesticide with a mass ratio of active component A to active component B of 1:2.5 Active component A 20%, active component B 50%, sodium lignin sulfonate 2%, organosilicon defoamer 1%, polyethylene glycol 3%, and the balance is water.

[0036] According to the formula, 20% of active component A, 50% of active component B, 2% of sodium lignin sulfonate, 3% of polyethylene glycol and half of the water are put into a stone mill and ground until the particle size is less than 3 microns. Then disperse them into a dispersion tank, add the remaining water and defoamer, and stir at high speed for 30 minutes.

[0037] Example 6 Pesticide with a mass ratio of active component A to active component B of 1:5 Active component A 10%, active component B 50%, sodium lignin sulfonate 2%, organosilicon defoamer 1%, polyethylene glycol 3%, and the balance is water.

[0038] The preparation method is the same as that of Example 5.

[0039] Comparative Example 1 Pesticides without Jinggangmycin Active component B 30%, antioxidant 3%, pH adjuster 2%, sodium lignin sulfonate 2%, polyethylene glycol 3%, sodium lignin sulfonate 5%, naphthalene sulfonate 3%, and the balance is lactic acid-glycolic acid copolymer.

[0040] The other steps are the same as those in Example 1.

[0041] Comparative Example 2 Pesticides without active ingredient B Jinggangmycin 20%, antioxidant 3%, pH adjuster 2%, sodium lignin sulfonate 2%, polyethylene glycol 3%, sodium lignin sulfonate 5%, naphthalene sulfonate 3%, and the balance is lactic acid-glycolic acid copolymer.

[0042] The preparation method is the same as that in Example 1.

[0043] Comparative Example 3 D-limonene was added to the xanthan gum aqueous solution at a formula mass ratio of 5:0.05, and after uniform stirring, insoluble impurities were filtered out and dried at 60° C. to obtain active component B.

[0044] Active component B 30%, antioxidant 3%, pH adjuster 2%, sodium lignin sulfonate 2%, polyethylene glycol 3%, sodium lignin sulfonate 5%, naphthalene sulfonate 3%, and the balance is lactic acid-glycolic acid copolymer.

[0045] The other steps are the same as those in Example 1.

[0046] Experimental Example 1 Determination of in vitro antibacterial activity of pesticides containing active component A and active component B in different mass ratios (Examples 1-6, Comparative Examples 1-2) against Rhizoctonia solani Rhizoctonia solani was provided by Zhejiang University and purchased from ATCC.

[0047] Test method: Refer to NY / T 1156.2-2006 "Guidelines for Indoor Bioassay Tests of Pesticides - Fungicides Part 2: Plate Method for Inhibition of Pathogenic Fungal Mycelial Growth".

[0048] (1) The original drug is first dissolved in dimethyl sulfoxide and then diluted into a single-dose stock solution; multiple groups of ratios are set, and each single-dose stock solution and the ratio mixture are set at a certain ratio, and each ratio is set with 5 mass concentration gradients.

[0049] (2) Mix different concentrations of the agent with the PDA medium, pour the plates to prepare plates containing different concentrations of the medium, use a 7 mm diameter puncher to cut the cultured Rhizoctonia solani, the pathogen of rice sheath blight, and inoculate it on the PDA plates containing the agent in series of concentrations; use the corresponding dimethyl sulfoxide as the control, and set up a blank control. Each treatment was repeated 3 times, and all treatments were cultured in a constant temperature incubator at 28°C.

[0050] (3) Record results and calculations: When the diameter of the blank control colony grows to about 2 / 3 of the diameter of the culture dish, measure the colony diameter using the cross method and calculate the inhibition rate of mycelial growth under different treatments. Convert the mycelial growth inhibition rate into a probability value (y), convert the drug concentration (mg / L) into a logarithmic value with a base of 10, and use DPS software to calculate the toxicity regression equation of each test drug, the effective inhibition concentration (EC50) and the correlation coefficient, and calculate the co-toxicity coefficient (CTC value) of the mixture according to the Sun Yunpei method.

[0051] Mycelium growth inhibition rate = [(control colony diameter - cake diameter) - (treated colony diameter - cake diameter)] / (control colony diameter - cake diameter) × 100%; Measured toxicity index (ATI) = (EC50 of standard agent ÷ EC50 of test agent) × 100; Theoretical toxicity index (TTI) = toxicity index of agent A × percentage of A in the mixture + toxicity index of agent B × percentage of B in the mixture; Co-toxicity coefficient (CTC) = [actual toxicity index of the mixture (ATI) ÷ theoretical toxicity index of the mixture (TTI)] × 100.

[0052] According to the classification standard of joint action: the co-toxicity coefficient (CTC) ≥ 120 shows synergistic effect; the co-toxicity coefficient (CTC) ≤ 80 shows antagonistic effect; 80 < co-toxicity coefficient (CTC) < 120 shows additive effect. The experimental results are shown in Table 1.

[0053] Table 1 Indoor bioactivity of pesticides with different mass ratios of A and B against Rhizoctonia solani

[0054] As can be seen from Table 1, the EC of active component A and active component B against Rhizoctonia solani of rice sheath blight 50 They were 18.72 mg / L and 39.25 mg / L respectively. When the mass ratio of active component A to active component B was in the range of 1: (0.4~5), the co-toxicity coefficient was greater than 120, showing a synergistic effect.

[0055] Experimental Example 2 Effects of pesticides with different mass ratios of active component A and active component B on rice disease control Field tests were conducted using Examples 1 to 6 and Comparative Examples 1 to 2 as examples to test the control effects of rice sheath blight, rice blast, bacterial leaf blight and rice false smut. The test was conducted at the rice test base in Fuyang District, Zhejiang Province. The test varieties were hybrid rice varieties. Each treatment was repeated 4 times, each repeated area was 4 square meters, and 5 points were investigated in double diagonal lines in each plot. All leaves of all rice plants in each point were investigated, and the occurrence of diseases was counted.

[0056] The severity of the disease is recorded using a grading method, and the grading standards are as follows: Level 0: no disease; Level 1: The area of ​​lesions is less than 5% of the entire leaf area; Level 3: The area of ​​lesions accounts for less than 6%-10% of the entire leaf area; Level 5: The area of ​​lesions accounts for less than 11%-20% of the entire leaf area; Level 7: The area of ​​lesions accounts for less than 21%-50% of the entire leaf area; Level 9: The area of ​​lesions accounts for more than 50% of the entire leaf area.

[0057] Calculation method of protection effect Diseased leaf rate (%) = number of diseased leaves / total number of leaves surveyed × 100% Disease index = ∑ (number of diseased leaves at each level × relative level value) / (total number of leaves investigated × 9) × 100 Control effect (%) = 〔1-(blank control area before drug disease index × treatment area after drug disease index) / (blank control area The disease index of the treated area after medication × the disease index of the treated area before medication) × 100%.

[0058] The pesticide was applied twice during the heading period of rice, with an interval of 10 days between each application, and the control effect was counted 7 days after the second application. After the test was completed, the control effect of rice sheath blight, rice blast and bacterial blight was focused on, and the efficacy was calculated. At the same time, the control effect of rice false smut was counted, and the statistics of rice false smut were increased once, and the statistical time was 20 days after the second application, and the control effect of rice false smut was counted as the final control effect data of rice false smut. The control effect data of rice false smut in the following table are the statistical data 20 days after the second application. The results are shown in Table 2.

[0059] Table 2 Statistics of field efficacy test results

[0060] As shown in Table 2, the pesticide prepared by the mass ratio of active component A to active component B is significantly higher than the single agent of active component A, Jinggangmycin and active component B, and the two have a synergistic effect in preventing and controlling rice diseases; the effect of active component B alone is close to Jinggangmycin, and its control effect is greatly reduced when active component B does not contain danshensu, indicating that danshensu, D-limonene and xanthan gum are compounded to have a synergistic effect on active component B, and are also the key factors for the interaction between active component A and active component B. It can also be seen from the above table that the compound pesticide of the present invention has excellent control effects on the four diseases of rice, that is, only the compound pesticide of the present invention can simultaneously control rice sheath blight, rice blast and bacterial leaf blight, thereby achieving the control effect of "one drug for multiple preventions", greatly reducing the use of chemical pesticides, reducing costs, and also reducing resistance to antibiotic pesticides, greatly improving the control efficiency of rice, which is very worthy of promotion.

[0061] The above embodiments are preferred implementation modes of the present invention, but the implementation modes of the present invention are not limited to the above embodiments. Any other changes, modifications, substitutions, combinations, and simplifications that do not deviate from the spirit and principles of the present invention should be equivalent replacement methods and are included in the protection scope of the present invention.

Claims

1. A compound pesticide containing Jinggangmycin, characterized in that: The invention is composed of the following components by mass percentage: 10-70% of active components, 1-3% of antioxidants, 1-2% of pH regulators, and the remainder of carriers and / or adjuvants; the active components include active component A: validamycin and active component B: danshensu, D-limonene and xanthan gum, and the mass ratio of active component A to active component B is 1:(0.4~5).

2. The compound pesticide containing Jinggangmycin according to claim 1, characterized in that: The mass ratio of the active component A to the active component B is 1:(0.5-2).

3. A compound pesticide containing Jinggangmycin according to claim 1 or 2, characterized in that: The mass ratio of the active component A to the active component B is 1:1.

5.

4. The compound pesticide containing Jinggangmycin according to claim 1, characterized in that: The mass ratio of danshensu, D-limonene and xanthan gum described in the active component B is 10:(4-6):0.

05.

5. The compound pesticide containing Jinggangmycin according to claim 1, characterized in that: The composition is composed of the following components by mass percentage: 35-50% of active component, 3% of antioxidant, 1% of pH adjuster, and the balance is carrier and / or auxiliary agent.

6. The compound pesticide containing Jinggangmycin according to claim 1, characterized in that: The auxiliary agent includes, but is not limited to, one or more of polyethylene glycol, glycerol, sodium dodecyl sulfate, sodium dodecylbenzene sulfonate, ammonium sulfate, alkylphenol polyoxyethylene ether, alkylphenol polyoxyethylene ether phosphate, fatty acid polyoxyethylene ester, benzoic acid, sodium lignin sulfonate, carboxymethyl cellulose or polyvinyl alcohol.

7. The compound pesticide containing Jinggangmycin according to claim 1, characterized in that: The carrier includes, but is not limited to, one or more of polyglycolic acid, lactic acid-glycolic acid copolymer, polyhydroxybutyric acid, polylactide, polycaprolactone, polyorthoester or polyalkyl cyanoacrylate.

8. The compound pesticide containing Jinggangmycin according to claim 1, characterized in that: The antioxidant includes but is not limited to any one of vitamin E, tert-butylhydroxytoluene, propyl gallate or sulfite.

9. The compound pesticide containing Jinggangmycin according to claim 1, characterized in that: The pH value of the pesticide is 5.5-7.

0.

10. Use of the compound pesticide containing Jinggangmycin according to claim 1 in preventing and controlling rice sheath blight, rice blast and bacterial leaf blight.

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