Oil-control soothing composition, application and washing care product

Abstract

The invention belongs to the technical field of hair care, and discloses an oil-controlling and soothing composition, application and a washing care product, the oil-controlling and soothing composition comprises a cowherb seed extract, a flaxseed extract, a fructus kochiae extract and a trollius chinensis bunge extract, the semen vaccariae extract, the flaxseed extract, the fructus kochiae extract and the trollflower extract are obtained by performing compound extraction on semen vaccariae, flaxseeds, fructus kochiae and trollflower, and the weight ratio of the semen vaccariae to the flaxseeds to the fructus kochiae to the trollflower is (1-3): (1-5): (0.5-2): (0.1-1.5). By compounding the cowherb seed extract, the flaxseed extract, the fructus kochiae extract and the trollius chinensis bunge extract, the oil control effect of the composition is synergistically improved in the directions of improving scalp metabolism, regulating scalp microcirculation, promoting hair follicle nutrition absorption and the like, and the effect of synergistically inhibiting 5 alpha-reductase can be generated.

Description

Technical Field

[0001] The invention relates to the technical field of hair care, and in particular to an oil-controlling and soothing composition, uses and a hair care product. Background Art

[0002] In modern society, due to factors such as eating habits and life pressure, hair loss among young people has gradually become a common phenomenon, and hair loss is mainly due to excessive oil secretion from the scalp, which leads to clogged hair follicles and corrosion of hair roots. Under normal circumstances, sebum has a protective effect on the scalp and hair, which can keep the scalp moisturized and make the hair shiny and smooth. However, if the sebaceous glands secrete excessive sebum due to irregular work and rest, staying up late, excessive mental stress, frequent consumption of oily and spicy foods, etc., the hair will produce excessive oil, which will cause the pores to become clogged and affect the breathing of the hair follicles, resulting in hair loss.

[0003] Therefore, oil control is not only to keep the scalp and hair clean and healthy, but also to prevent possible scalp problems and hair loss.

[0004] The oil secretion of the scalp is closely related to 5α-reductase. 5α-reductase is a protease. Its abnormal activity or increase will stimulate the conversion of testosterone into dihydrotestosterone (DHT) in the body. The increase of 5α-reductase will convert male hormones into dihydrotestosterone. Dihydrotestosterone binds to the androgen receptors in the sebaceous glands, causing the sebaceous glands to fall into a state of over-secretion, thus leading to excessive oil secretion on the scalp. In addition, high levels of dihydrotestosterone will also affect the cell function of hair follicles, resulting in a shortened growth cycle of hair follicles, smaller hair follicles, and atrophy of hair follicles, which may eventually lead to problems such as hair loss.

[0005] Therefore, the inhibition of 5α-reductase is the key research and development direction of hair care products.

[0006] For example, prior art 1: Chinese patent application 202411354989.6 discloses a method and application of fermenting Platycladus orientalis leaves with composite bacteria, wherein the composite bacteria comprises Lactococcus lactis and Pichia pastoris; the method comprises the following steps: (1) pretreatment of Platycladus orientalis leaves; (2) enzyme treatment of Platycladus orientalis leaves; (3) fermentation of Platycladus orientalis leaves; and (4) fermentation liquid treatment. The specification of the patent application states: "Using the optimized fermentation method, more flavonoids can be obtained, with better anti-hair loss effect, which is more conducive to the application of Platycladus orientalis leaves"; and the specification of the patent application also experimentally verifies the effect of Platycladus orientalis leaf fermentation products in inhibiting 5α-reductase.

[0007] Similarly, prior art 2: Chinese patent application 2024113149408 discloses the use of plant extracts in the preparation of oil control and anti-hair loss products, and the plant extracts contain Ganoderma triterpenes; its specification states: "Many active ingredients in plants, such as triterpenes, flavonoids, alkaloids, etc., have multiple biological activities such as antioxidant, anti-inflammatory, and antibacterial, which are helpful to scalp health, thereby improving hair loss to a certain extent"; this patent application inhibits 5α-reductase by extracting Ganoderma triterpenes, and then applies them to anti-hair loss shampoo and conditioner products.

[0008] Prior art 2 shows that the active ingredients of many plants have the effect of inhibiting 5α-reductase and improving hair loss; at the same time, prior art 1 also verifies the authenticity of this theory. However, since the content of a single ingredient in a hair care product needs to be limited to ensure safe use, the anti-hair loss effect of using only a single component is weak. Prior art 1 and prior art 2 have not studied in depth how to improve the inhibitory effect of 5α-reductase by combining different active ingredients from multiple plants. Summary of the invention

[0009] One of the purposes of the present invention is to provide an oil-controlling and soothing composition to avoid the problem that the shampoo and conditioner products rely solely on a single component and have a weak 5α-reductase inhibitory effect, and to effectively improve the 5α-reductase inhibitory effect of the composition by combining multiple components.

[0010] Another object of the present invention is to provide a method for preparing an oil-controlling and soothing composition, wherein the active ingredients of fenugreek, linseed, Kochia scoparia and nasturtium are extracted through composite extraction to achieve excellent 5α-reductase inhibitory effect.

[0011] At the same time, the present invention also provides the use of the oil-controlling and soothing composition, which is used to prepare a cleaning product to obtain a cleaning product with excellent oil-controlling and soothing improvement conditions.

[0012] Furthermore, the present invention also provides a shampoo containing a certain amount of an oil-controlling and soothing composition, which effectively improves the oil-controlling and soothing effect of the shampoo by compounding multiple components under the condition of limiting the amount of a single component.

[0013] To achieve the above-mentioned purpose, the present invention provides an oil-controlling and soothing composition, comprising fenugreek extract, flaxseed extract, kochia scoparia extract and nasturtium extract, wherein the fenugreek extract, flaxseed extract, kochia scoparia extract and nasturtium extract are obtained by composite extraction of fenugreek, flaxseed, kochia scoparia and nasturtium, and the weight ratio of the fenugreek, flaxseed, kochia scoparia and nasturtium is 1-3:1-5:0.5-2:0.1-1.5.

[0014] Fenugreek extract is rich in a variety of active ingredients. When these ingredients act on the scalp, they can effectively reduce the production of excessive oil and keep the scalp refreshed. At the same time, it can also refine pores, improve the scalp environment, and promote nutrient absorption by hair follicles, making the scalp look healthier and more delicate.

[0015] Flaxseed extract is rich in unsaturated fatty acids. When acting on the scalp, these ingredients can improve the metabolism of the scalp and make the stratum corneum of the scalp more resilient, thus helping to control the secretion of oil. At the same time, unsaturated fatty acids also have a certain antioxidant effect, which is beneficial to hair growth and can assist in the treatment of hair loss caused by seborrheic dermatitis. In addition, essential fatty acids such as Omega-3 and Omega-6 in flaxseed extract can also promote scalp microcirculation and provide sufficient nutrition for hair follicles. This nutrient delivery helps hair growth, making hair thicker and more lustrous, which also helps to control oil to a certain extent.

[0016] The saponin component rich in Kochia scoparia seed extract has a good cleansing effect and can remove dirt and oil on the scalp. At the same time, its antioxidant and anti-inflammatory effects also help to improve the scalp environment and thus control oil secretion.

[0017] Although nasturtium extract does not have a clear oil-control effect, it has the effect of nourishing hair and enhancing hair elasticity; and after being combined with fenugreek extract, linseed extract, and Kochia scoparia extract, the oil-control effect of the composition can be significantly improved.

[0018] The present invention, through the compounding of fenugreek extract, linseed extract, Kochia scoparia extract and nasturtium extract, synergistically enhances the oil control effect of the composition from the directions of improving scalp metabolism, regulating scalp microcirculation, and promoting nutrient absorption of hair follicles, and can produce a synergistic inhibition of 5α-reductase.

[0019] In addition, the combination of fenugreek extract, flaxseed extract, Kochia scoparia extract and nasturtium extract synergistically enhances the soothing effect of the composition. ‌‌ The composite extraction of the fenugreek extract, linseed extract, Kochia scoparia extract and nasturtium extract comprises the following steps: Step 1: Add fenugreek, linseed, Kochia scoparia and nasturtium to water, stir at 60-70° C. for 3-4 hours to obtain a mixture, and centrifuge the mixture to obtain an extract.

[0020] Step 2: Concentrate the extract to 20-30% of the original mass at 65±5°C and -0.06~-0.1MPa to obtain a concentrated solution; Step 3: Add an equal amount of 95% ethanol to the concentrated solution for alcohol precipitation, and separate the solid and liquid to obtain an alcohol precipitate; Step 4: The alcohol precipitate is transferred to a falling film concentrator, and the filtrate is vacuum concentrated to zero ethanol content at a concentration temperature of 60±5°C and a concentration pressure of -0.06~-0.1MPa to obtain an alcohol-free concentrate; Step 5: adding butanediol and pure water to the alcohol-free concentrate to perform ultrafiltration to obtain an ultrafiltrate, wherein the amount of butanediol added is 1-15 times the mass of the alcohol-free concentrate, and the amount of pure water added is 2-20 times the mass of the alcohol-free concentrate.

[0021] Preferably, the pore size of the ultrafiltration membrane in the ultrafiltration in step 5 is 0.05-0.45 um, the ultrafiltration pressure is 0.1-0.3 MPa, and the ultrafiltration temperature is 30±10°C.

[0022] Furthermore, the method further comprises step 6: adding a preservative to the ultrafiltrate for sterilization.

[0023] The preservative is at least one of p-hydroxyacetophenone, hexylene glycol, caprylhydroxamic acid, ethylhexylglycerin, pentylene glycol, sodium benzoate and phenoxyethanol.

[0024] The present invention also provides a use of the oil-controlling and soothing composition in preparing cleaning products.

[0025] Preferably, the hair care products include shampoo, shampoo cream, conditioner, essence, lotion, cream, hair care essential oil, and dry hair spray.

[0026] Furthermore, the present invention also provides a cleaning product comprising 0.1-30 wt % of the oil-controlling and soothing composition.

[0027] Compared with the prior art, the present invention has the following beneficial effects: (1) The present invention uses a compound of fenugreek extract, linseed extract, Kochia scoparia extract and nasturtium flower extract to synergistically enhance the oil control effect of the composition from the perspectives of improving scalp metabolism, regulating scalp microcirculation, and promoting nutrient absorption of hair follicles, thereby producing a synergistic inhibitory effect on 5α-reductase; (2) The present invention uses a combination of fenugreek extract, linseed extract, Kochia scoparia extract and nasturtium flower extract to synergistically enhance the soothing effect of the composition. BRIEF DESCRIPTION OF THE DRAWINGS

[0028] Figure 1 is a picture of a chicken embryo before using the oil-controlling and soothing composition of Example 6 of the present invention; Figure 2 This is a picture of a chick embryo after using the oil-controlling and soothing composition of Example 6 of the present invention. DETAILED DESCRIPTION

[0029] The present invention will be further described below in conjunction with the embodiments, but it does not constitute any limitation to the present invention. Any limited number of modifications made within the scope of the claims of the present invention are still within the scope of the claims of the present invention.

[0030] In order to explain the technical content of the present invention in detail, further description is given below in conjunction with the implementation modes.

[0031] In the following examples and comparative examples, the properties of the fenugreek, linseed, Kochia scoparia, nasturtium, witch hazel, olive leaf, sophora flavescens, and rosemary are all powders after being crushed and sieved through a 100-mesh sieve.

[0032] Unless otherwise specified, the parts described in the following examples and comparative examples are all parts by weight.

[0033] In the following examples and comparative examples, the compositions are obtained by composite extraction using the following steps: Step 1: Add fenugreek or witch hazel, linseed or olive leaf, Kochia scoparia or Sophora flavescens, Nasturtium or rosemary into water, stir at 65±2° C. for 3-4 hours to obtain a mixture, and centrifuge the mixture to obtain an extract.

[0034] Step 2: Concentrate the extract to 20% of the original mass at 65±5°C and -0.08MPa to obtain a concentrated solution; Step 3: Add an equal amount of 95% ethanol to the concentrated solution for alcohol precipitation, and separate the solid and liquid to obtain an alcohol precipitate; Step 4: The alcohol precipitate is transferred to a falling film concentrator, and the filtrate is vacuum concentrated to zero ethanol content at a concentration temperature of 60±5° C. and a concentration pressure of -0.08 MPa to obtain an alcohol-free concentrate; Step 5: Add 1.5 times the mass of butanediol and 2.5 times the mass of pure water to the alcohol-free concentrate, and then use an ultrafiltration membrane with a pore size of 0.25um to perform ultrafiltration at an ultrafiltration pressure of 0.2MPa and an ultrafiltration temperature of 30±10°C to obtain an ultrafiltrate.

[0035] Example 1 An oil-controlling and soothing composition is obtained by composite extraction of 3 parts of fenugreek, 2 parts of linseed, 0.5 parts of kochia scoparia and 0.2 parts of nasturtium.

[0036] Example 2 An oil-controlling and soothing composition is obtained by composite extraction of 2 parts of fenugreek, 4 parts of linseed, 1 part of kochia scoparia and 1 part of nasturtium.

[0037] Example 3 An oil-controlling and soothing composition is obtained by composite extraction of 1 part of fenugreek, 5 parts of linseed, 2 parts of kochia seeds and 0.5 part of nasturtium.

[0038] Example 4 An oil-controlling and soothing composition is obtained by composite extraction of 3 parts of fenugreek, 1 part of linseed, 1.5 parts of kochia scoparia and 1.5 parts of nasturtium.

[0039] Example 5 An oil-controlling and soothing composition is obtained by composite extraction of 2 parts of fenugreek, 1 part of linseed, 2 parts of kochia scoparia and 0.9 part of nasturtium.

[0040] Example 6 An oil-controlling and soothing composition is obtained by composite extraction of 3 parts of fenugreek, 3 parts of linseed, 3 parts of kochia seeds and 3 parts of nasturtium.

[0041] Comparative Example 1 An oil-controlling and soothing composition is obtained by composite extraction of 4 parts of fenugreek, 4 parts of linseed and 4 parts of kochia scoparia.

[0042] Comparative Example 2 An oil-controlling and soothing composition is obtained by composite extraction of 4.5 parts of fenugreek, 4.5 parts of linseed and 3 parts of kochia scoparia.

[0043] Comparative Example 3 An oil-controlling and soothing composition is obtained by composite extraction of 4 parts of fenugreek, 4 parts of linseed and 4 parts of nasturtium.

[0044] Comparative Example 4 An oil-controlling and soothing composition is obtained by composite extraction of 4.5 parts of fenugreek, 4.5 parts of linseed and 3 parts of nasturtium.

[0045] Comparative Example 5 An oil-controlling and soothing composition is obtained by composite extraction of 4 parts of flax seeds, 4 parts of Kochia scoparia seeds and 4 parts of nasturtium.

[0046] Comparative Example 6 An oil-controlling and soothing composition is obtained by composite extraction of 4.5 parts of flaxseeds, 4.5 parts of Kochia scoparia seeds and 3 parts of nasturtium.

[0047] Comparative Example 7 An oil-controlling and soothing composition is obtained by composite extraction of 4.5 parts of flaxseeds, 3 parts of Kochia scoparia seeds and 4.5 parts of nasturtium.

[0048] Comparative Example 8 An oil-controlling and soothing composition is obtained by composite extraction of 4 parts of fenugreek, 4 parts of kochia scoparia and 4 parts of nasturtium.

[0049] Comparative Example 9 An oil-controlling and soothing composition is obtained by composite extraction of 3 parts of witch hazel, 3 parts of linseed, 3 parts of kochia seeds and 3 parts of nasturtium.

[0050] Comparative Example 10 An oil-controlling and soothing composition is obtained by composite extraction of 3 parts of fenugreek, 3 parts of olive leaves, 3 parts of kochia seeds and 3 parts of nasturtium.

[0051] Comparative Example 11 An oil-controlling and soothing composition is obtained by composite extraction of 3 parts of fenugreek, 3 parts of linseed, 3 parts of sophora flavescens and 3 parts of nasturtium.

[0052] Comparative Example 12 An oil-controlling and soothing composition is obtained by composite extraction of 3 parts of fenugreek, 3 parts of linseed, 3 parts of kochia scoparia and 3 parts of rosemary.

[0053] Efficacy Test 1. 5α-reductase inhibition rate test Test method: 1. Experimental purpose and principle Oily skin has more active secretion of sebaceous glands, which makes the face feel greasy, difficult to clean, and has a glossy appearance that affects appearance. At the same time, some corresponding skin diseases are also prone to occur, such as acne vulgaris and seborrheic dermatitis. In view of the relationship between 5α-reductase and sebaceous glands, the inhibitory effect of the test sample on 5α-reductase is usually tested to reflect the sebum regulation effect, so as to characterize the oil control effect of the test sample.

[0054] This test refers to the laboratory method and compares the 5α-reductase inhibition rate test results of the test sample with that of the negative control. If the inhibition rate of the test sample is higher than that of the negative control and there is a significant difference (P<0.05), it can be considered that the test sample has a certain oil control effect.

[0055] This test method is an in vitro method and is suitable for evaluating cosmetic products that claim to achieve oil control by inhibiting 5α-reductase.

[0056] 2 Test indicators 5α-reductase inhibition rate determination standard: If the 5α-reductase inhibition rate of the sample is higher than that of the negative control and there is a significant difference (P<0.05), it can be considered that the test sample has a certain oil control effect.

[0057] 3 Experimental materials and methods Test Materials: testosterone, 98%; NADPH, 90%; Methanol, HPLC grade; Buffer: Tris-HCl.

[0058] Test methods (1) Treatment of control and test samples Sample group: diluted with buffer to a sample concentration of 5%; Positive control (finasteride, purity ≥98%): dilute with methanol to a positive control concentration of 0.1%; Negative control: Tris-HCl buffer.

[0059] (2) Test operation steps A sample group, a positive control group, a negative control group and a blank group were set up. Three parallels were set up for each group. Different reagent solutions were added to the four groups respectively, and the mixture was shaken well. Each group was subjected to HPLC analysis to determine the testosterone content in each tube.

[0060] (3) Calculation formula .

[0061] (4) Data analysis The statistical analysis software was SPSS, and the comparison of 5α-reductase inhibition rates among the test samples, positive controls, and negative controls was performed using an independent sample t-test. The above statistical analyses were all two-tailed tests, with a significance level of α=0.05. P>0.05 indicated that there was no significant difference between the two groups; P<0.05 indicated that there was a significant difference between the two groups.

[0062] The oil-controlling and soothing compositions of Examples 1-6 and Comparative Examples 1-12 were tested according to the above method. The results are shown in Table 1.

[0063] Table 1 Test results of 5α-reductase inhibition rate of the oil-controlling and soothing compositions of Examples 1-6 and Comparative Examples 1-12 5α-reductase inhibition rate% P-value Example 1 33.645 P<0.05 Example 2 36.198 P<0.05 Example 3 32.957 P<0.05 Example 4 33.211 P<0.05 Example 5 33.645 P<0.05 Example 6 35.799 P<0.05 Comparative Example 1 22.634 P<0.05 Comparative Example 2 24.268 P<0.05 Comparative Example 3 19.823 P<0.05 Comparative Example 4 22.463 P<0.05 Comparative Example 5 16.106 P<0.05 Comparative Example 6 16.529 P<0.05 Comparative Example 7 16.288 P<0.05 Comparative Example 8 16.430 P<0.05 Comparative Example 9 16.731 P<0.05 Comparative Example 10 16.912 P<0.05 Comparative Example 11 18.498 P<0.05 Comparative Example 12 18.335 P<0.05 Positive Control 66.916 P<0.05 Negative control 2.033 /

[0064] According to the results in Table 1, we can see that: According to the data of Examples 1-6, the present application adopts a compound of fenugreek extract, linseed extract, Kochia scoparia extract and Nasturtium flower extract, which can make the 5α-reductase inhibition rate of the composition reach more than 32%, and has excellent oil control effect, among which the effect of Example 2 is better.

[0065] According to the data comparison between Example 6 and Comparative Example 1, in the absence of Nasturtium, even if the total amount of raw materials is the same, the 5α-reductase inhibition rate of the composition formed by the composite extract obtained in Comparative Example 1 is significantly lower than that in Example 1; indicating that in the technical scheme of the present application, Nasturtium plays an important role; similarly, according to the data comparison between Example 3, in the absence of Kochia scoparia, even if the total amount of raw materials is the same, the 5α-reductase inhibition rate of the composition formed by the composite extract obtained is significantly lower than that in Example 1; similarly, the technical schemes of Comparative Example 5 lacking Vaccaria segetalis and Comparative Example 8 lacking flaxseed also show a significant downward trend; it can be seen that the technical scheme of the present application is to produce a synergistic effect of improving the 5α-reductase inhibition rate of the composition by compounding Vaccaria segetalis extract, flaxseed extract, Kochia scoparia extract and Nasturtium flower extract.

[0066] According to the data comparison of Comparative Example 1 and Comparative Example 2, under the same total dosage, the dosage of Kochia scoparia slightly reduced and the dosage of Vaccaria segetalis and linseed increased accordingly, the 5α-reductase inhibition rate of the composition was improved to a certain extent, which is speculated to be one of the following reasons: (1) The 5α-reductase inhibition rate of fenugreek and flaxseed is significantly higher than that of Kochia scoparia, so there is a certain improvement after adjusting the dosage; (2) Vaccaria segetalis and flaxseed have a certain degree of synergistic effect when combined, but the synergistic effect is not obvious enough. Therefore, after adjusting the dosage, the synergistic effect is further improved, thereby improving the 5α-reductase inhibition rate of the combination.

[0067] In order to verify which reason it is, the present application verifies the difference brought by the technical solution of increasing the dosage of flaxseed and Kochia scoparia in Comparative Example 5 through Comparative Example 6. According to the data comparison between Comparative Example 6 and Comparative Example 5, it can be seen that increasing the dosage of flaxseed and Kochia scoparia simultaneously in Comparative Example 6 cannot significantly improve the 5α-reductase inhibition rate of the composition; combined with the data comparison between Comparative Example 8 and Comparative Example 5, it can be seen that the 5α-reductase inhibition rate of Vaccaria segetalis is not much higher than that of flaxseed, thus it can be determined that Vaccaria segetalis and flaxseed have a certain compound synergy, but the compound synergistic effect is not obvious enough.

[0068] At the same time, according to the comparison of the data of Comparative Example 3 and Comparative Example 4, under the condition of the same total dosage, the dosage of Nasturtium is slightly reduced and the dosage of Vaccaria segetalis and linseed is correspondingly increased, and the 5α-reductase inhibition rate of the composition is improved to a certain extent; and one thing to note is that the improvement of the 5α-reductase inhibition rate shown in Comparative Example 4 compared with Comparative Example 3 is much higher than the improvement of the 5α-reductase inhibition rate shown in Comparative Example 2 compared with Comparative Example 1. Based on this, it is reasonable to speculate that the 5α-reductase inhibition effect of Nasturtium itself is very weak.

[0069] Therefore, Comparative Example 7, Comparative Example 5 and Comparative Example 6 also indirectly verify this conclusion. Compared with Comparative Example 6, Comparative Example 7 increases the amount of nasturtium and reduces the amount of Kochia scoparia, but the 5α-reductase inhibition rate of the composition decreases to a certain extent; and when the amount of flaxseed in Comparative Example 7 is higher than that in Comparative Example 5, the 5α-reductase inhibition rate shown by the composition does not increase much. It can be seen that the 5α-reductase inhibition effect of nasturtium itself is very weak, at least much lower than that of Kochia scoparia and flaxseed.

[0070] However, according to the data comparison of Example 6 and Comparative Examples 1 and 2, even though the 5α-reductase inhibition effect of Nasturtium itself is very weak, the 5α-reductase inhibition effect exhibited by the combination of fenugreek, linseed, Kochia seeds and Nasturtium can be significantly higher than the combination of fenugreek, linseed and Kochia seeds, which fully demonstrates that the combination of fenugreek extract, linseed extract, Kochia seeds extract and Nasturtium extract has a synergistic effect of enhancing the 5α-reductase inhibition of the composition.

[0071] According to the data comparison of Example 6 and Comparative Examples 9-12, in the absence of any one of fenugreek, flaxseed, Kochia seeds, and Nasturtium, even if Hamamelis virginiana with similar efficacy to fenugreek is used to replace fenugreek, olive leaves with similar efficacy to flaxseed are used to replace flaxseed, Sophora flavescens with similar efficacy to Kochia seeds are used to replace Kochia seeds, and rosemary with similar efficacy to Nasturtium is used to replace Nasturtium, the 5α-reductase inhibition rate shown by the compositions of Comparative Examples 9-12 all showed a significant decrease, indicating that the present application shows a synergistic improvement of the 5α-reductase inhibition of the composition through the compound of fenugreek extract, flaxseed extract, Kochia seeds extract, and Nasturtium extract, and none of the four is indispensable; carefully comparing Comparative Examples 11 and 12 with Comparative Examples 9 and 10, it can be seen that Comparative Examples 11 and 12 will have a certain improvement when fenugreek and flaxseed are present at the same time. Combined with the previous experiments, it can be further determined that fenugreek and flaxseed have a certain compound synergy, but the compound synergistic effect is not obvious enough.

[0072] 2. Hyaluronidase inhibition rate test 1. Purpose and principle of the experiment Hyaluronidase is a specific cleavage enzyme of hyaluronic acid, a participant in allergic reactions, and has a strong correlation with the release of histamine by mast cells. Whether the test sample has a soothing effect can be judged by the hyaluronidase inhibition rate. The higher the hyaluronidase inhibition rate, the stronger the soothing effect of the substance, and vice versa.

[0073] This test refers to the laboratory method (HMC-WI-029 hyaluronidase inhibition rate) and compares the hyaluronidase inhibition rate test results of the test sample with that of the negative control. If the inhibition rate of the test sample is higher than that of the negative control and there is a significant difference (P<0.05), it can be considered that the test sample has a certain soothing effect.

[0074] This test method is an in vitro method suitable for evaluating cosmetic products that claim to achieve soothing effects by inhibiting hyaluronidase.

[0075] 2 Test indicators Hyaluronidase inhibition rate determination criteria: If the hyaluronidase inhibition rate of the sample is higher than that of the negative control and there is a significant difference (P<0.05), then it can be considered that the test sample has a certain soothing effect.

[0076] 3 Experimental materials and methods 3.1 Instruments and equipment: BSA224S analytical balance; L6s UV spectrophotometer.

[0077] 3.2 Reagents Hyaluronidase, BR; Sodium hyaluronate, BR.

[0078] 3.3 Test methods (1) Treatment of control and test samples Sample group: Dilute with pure water to a sample concentration of 5%; Positive control (dipotassium glycyrrhizinate, purity ≥98%): diluted with water to a positive control concentration of 3%; Negative control: pure water.

[0079] (2) Test operation steps Set up a sample group, a sample background group, a solvent group and a solvent background group. Each group needs to have 3 parallels. Add different reagent solutions to the four groups respectively, shake well, and leave at room temperature for 30 minutes for color development. Use a UV spectrophotometer to measure the absorbance value at a wavelength of 528nm.

[0080] (3) Calculation formula .

[0081] Where: A—is the absorbance of the reaction solution without sample; B—is the absorbance of the reaction solution without sample and enzyme; C—is the absorbance of the reaction solution containing sample and enzyme; D—is the absorbance of the reaction solution containing sample and without enzyme.

[0082] (4) Data analysis SPSS was used as the statistical analysis software, and the independent sample t-test was used to compare the hyaluronidase inhibition rates among the test samples, positive controls, and negative controls.

[0083] The above statistical analyses were all two-tailed tests, with a significance level of α = 0.05. P>0.05 means there is no significant difference between the two groups; P<0.05 means there is a significant difference between the two groups.

[0084] The oil-controlling and soothing compositions of Examples 1-6 and Comparative Examples 1-12 were tested according to the above method. The results are shown in Table 2.

[0085] Table 2 Test results of hyaluronidase inhibition rate of the oil-controlling and soothing compositions of Examples 1-6 and Comparative Examples 1-12 Hyaluronidase inhibition rate% P-value Example 1 52.604 P<0.05 Example 2 53.399 P<0.05 Example 3 53.861 P<0.05 Example 4 54.947 P<0.05 Example 5 53.258 P<0.05 Example 6 57.582 P<0.05 Comparative Example 1 48.314 P<0.05 Comparative Example 2 47.047 P<0.05 Comparative Example 3 45.662 P<0.05 Comparative Example 4 44.878 P<0.05 Comparative Example 5 50.470 P<0.05 Comparative Example 6 48.915 P<0.05 Comparative Example 7 48.034 P<0.05 Comparative Example 8 49.237 P<0.05 Comparative Example 9 47.463 P<0.05 Comparative Example 10 46.575 P<0.05 Comparative Example 11 50.686 P<0.05 Comparative Example 12 46.932 P<0.05 Positive Control 68.338 P<0.05 Negative control -0.528 /

[0086] According to the results in Table 2, we can see that: According to the data of Examples 1 to 6, the oil-controlling and soothing composition of the present invention has an excellent hyaluronidase inhibition rate, but the amount of each component fluctuates greatly, among which the scheme of Example 6 has the better effect.

[0087] According to the data comparison of Example 6 and Comparative Examples 1-8, it can be seen that in the absence of any one of fenugreek, linseed, Kochia seeds, and Nasturtium, the hyaluronidase inhibition rate of the prepared composition is significantly reduced, indicating that the present application also has the effect of synergistically improving the soothing effect of the composition through the compounding of fenugreek extract, linseed extract, Kochia seeds extract and Nasturtium extract; however, according to the data of Comparative Examples 5 and 8, it can be seen that the synergistic effect of the composition of the present application in the soothing effect is lower than the 5α-reductase inhibition effect; combined with the data comparison of Example 1 and Example 6, it can be seen that the synergistic effect of improving the hyaluronidase inhibition rate produced by the compounding of fenugreek extract, linseed extract, Kochia seeds extract and Nasturtium extract in the present application is relatively limited, but still has a synergistic effect.

[0088] From the comparison between the data of Comparative Examples 1-8, it can be known that in the absence of any one of fenugreek, linseed, Kochia scoparia and Nasturtium, the prepared composition only has the effect of superimposing the effects; especially according to the data comparison between Comparative Example 1 and Comparative Example 2, the data comparison between Comparative Example 3 and Comparative Example 4, the data comparison between Comparative Example 5 and Comparative Examples 1-4, the data comparison between Comparative Example 5 and Comparative Example 6, and the data comparison between Comparative Example 7 and Comparative Example 8, it can be inferred that: in terms of soothing effect, the effects of the extracts of the four plants are ranked as Kochia scoparia>Nasturtium>linseed>fenugreek.

[0089] According to the data comparison between Comparative Example 1 and Comparative Examples 9-12, it can be seen that the present application produces a synergistic effect of improving the hyaluronidase inhibition rate through the compounding of fenugreek extract, linseed extract, Kochia scoparia extract and Nasturtium extract, while other plants cannot obtain the corresponding synergistic effect of improving the soothing effect; however, according to the data of Comparative Example 11, it can be seen that the hyaluronidase inhibition rate shown by Sophora flavescens is not inferior to that of Kochia scoparia.

[0090] 3. Irritation Test Test method: Chicken embryo chorioallantoic membrane test 1. Purpose and principle of the test The chicken embryo chorioallantoic membrane test is an early in vitro eye irritation assessment method. The chorioallantoic membrane (CAM) is a respiratory membrane that surrounds the chicken embryo. This test uses the characteristics of the intact, clear and transparent vascular system of the chorioallantoic membrane of the mid-stage incubated chicken embryo. A certain amount of the test substance is directly exposed to the chicken embryo chorioallantoic membrane. After a period of exposure, the changes in the toxic effect indicators of the chorioallantoic membrane (such as bleeding, coagulation and vascular melting) are observed. These indicators reflect the changes in the morphological structure, color and permeability of the blood vessels and vascular networks, as well as the phenomena such as the denaturation of the chorioallantoic membrane proteins and the degree of damage. Then a score is obtained by combining them to evaluate the eye irritation of the test substance.

[0091] The purpose of this test is to test the ability of the test substance to cause toxic changes in the chicken embryo chorioallantoic membrane and to evaluate the elements and processes of the potential eye irritation of the evaluated substance.

[0092] 2. Experimental Group Treatment and Reagents Sample group: the oil-controlling and soothing compositions prepared in Examples 1 to 6 and Comparative Examples 1 to 12 were diluted into 2% aqueous solutions; Negative control: 0.9% saline; Positive control: 1% sodium dodecyl sulfate (SDS) solution.

[0093] 3. Experimental steps In this test, 6 chicken embryos were selected for each group, and the conditions of the chorioallantoic membrane were recorded with a camera. A polytetrafluoroethylene resin ring was placed on the chorioallantoic membrane of the chicken embryo and photographed. The sample to be tested was added to the polytetrafluoroethylene resin ring, the time of adding the sample was recorded, and the air chamber was covered with a moistened plastic wrap. The chicken embryo was moved to a constant temperature and humidity chamber for culture, and the degree of change of each toxic effect was observed.

[0094] 4. Calculation of results The endpoint evaluation method was used to calculate the endpoint evaluation (ES) with the result retained to two decimal places; each chicken embryo was scored as the sum of the observed bleeding, coagulation and vascular melting degrees for each chicken embryo; the average value of the mathematical sum obtained for ES-6 chicken embryos was taken.

[0095] The average ES score is calculated according to the following formula: .

[0096] 5. Result judgment criteria ES≤4, non-irritating; 4<ES≤12, mild irritation; 12<ES<16, moderate irritation; ES ≥ 16, strong irritant / corrosive.

[0097] 2. Test Results According to the above test method, samples of Examples 1-6 and Comparative Examples 1-12 were tested, and the ES values ​​were all less than 4, and the results were all judged to be non-irritating.

[0098] Among them, the results of chicken embryos before and after use in Example 6 are as follows Figure 1 , Figure 2 As shown; This proves that the technical solution of the present invention is mild and non-irritating.

[0099] Application Examples A shampoo, the formula ratio of which is shown in Table 3, is prepared according to the following steps: 1. Clean and disinfect the necessary equipment and utensils; Prefabricated phase: Phase D is pre-dispersed evenly; Phase E is pre-dissolved completely; 2. After cleaning the pot, add phase A pure water, add JK-140E under homogenization conditions, and after homogenization and dispersion, heat it to 62±2℃, add anhydrous citric acid, heat it to 80-85℃ and stir for 15-20min; 3. Add the raw materials of phase B in sequence at 82±2℃, keep warm and stir for 30min until they are completely dissolved, then start to cool down; 4. When the temperature starts to drop, add phase C into the emulsifying pot and stir to dissolve evenly; 5. Cool down to 60℃, add pre-dispersed phase D raw materials and stir evenly; 6. Cool down to 45℃, add pre-dispersed phase E and phase F into the emulsifier in sequence, disperse and dissolve, and stir evenly.

[0100] Table 3 Recipe ratio table

[0101] The embodiments presented herein are only embodiments selected according to the combination of all possible embodiments. The appended claims should not be limited by the embodiments describing the present invention. Some numerical ranges used in the claims include sub-ranges therein, and the changes in these ranges should also be covered by the appended claims.

Claims

1. An oil-controlling and soothing composition, characterized in that: The invention comprises fenugreek extract, flaxseed extract, kochia scoparia extract and nasturtium extract, wherein the fenugreek extract, flaxseed extract, kochia scoparia extract and nasturtium extract are obtained by composite extraction of fenugreek, flaxseed, kochia scoparia and nasturtium, and the weight ratio of fenugreek, flaxseed, kochia scoparia and nasturtium is 1-3:1-5:0.5-2:0.1-1.

5.

2. The oil control soothing composition according to claim 1, characterized in that: The composite extraction of the fenugreek extract, linseed extract, Kochia scoparia extract and nasturtium extract comprises the following steps: Step 1: adding fenugreek, linseed, Kochia scoparia and nasturtium to water, stirring at 60-70° C. for 3-4 hours to obtain a mixture, and centrifuging the mixture to obtain an extract; Step 2: Concentrate the extract to 20-30% of the original mass at 65±5°C and -0.06~-0.1MPa to obtain a concentrated solution; Step 3: Add an equal amount of 95% ethanol to the concentrated solution for alcohol precipitation, and separate the solid and liquid to obtain an alcohol precipitate; Step 4: The alcohol precipitate is transferred to a falling film concentrator, and the filtrate is vacuum concentrated to zero ethanol content at a concentration temperature of 60±5°C and a concentration pressure of -0.06~-0.1MPa to obtain an alcohol-free concentrate; Step 5: adding butanediol and pure water to the alcohol-free concentrate to perform ultrafiltration to obtain an ultrafiltrate, wherein the amount of butanediol added is 1-15 times the mass of the alcohol-free concentrate, and the amount of pure water added is 2-20 times the mass of the alcohol-free concentrate.

3. The oil control soothing composition according to claim 2, characterized in that: The pore size of the ultrafiltration membrane in the ultrafiltration in step 5 is 0.05-0.45um, the ultrafiltration pressure is 0.1-0.3MPa, and the ultrafiltration temperature is 30±10°C.

4. The oil control soothing composition according to claim 2, characterized in that: The method also includes step 6: adding a preservative to the ultrafiltrate for sterilization.

5. The oil control soothing composition according to claim 4, characterized in that: The preservative is at least one of p-hydroxyacetophenone, hexylene glycol, caprylhydroxamic acid, ethylhexylglycerin, pentylene glycol, sodium benzoate and phenoxyethanol.

6. Use of the oil-controlling and soothing composition according to any one of claims 1 to 5 in preparing cleaning products.

7. Use of the oil-controlling and soothing composition according to claim 6 for preparing a cleaning product, characterized in that: The cleaning and care products include shampoo, shampoo cream, conditioner, essence, lotion, cream, hair care essential oil and dry hair spray.

8. A cleaning product, characterized in that: Contains 0.1-30wt% of the oil-controlling and soothing composition according to any one of claims 1-5.

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