Anti-hair-loss composition containing picea norvegensis leaf extract and application of anti-hair-loss composition

Abstract

The invention discloses an anti-hair loss composition containing picea norvegensis leaf extract and application, and relates to the technical field of cosmetics. The composition disclosed by the invention comprises a component A and a component B, wherein the component A comprises the following components in parts by weight: 1-5 parts of a verbasci extract, 0.1-1 part of a saw palmetto extract and 1-5 parts of a picea norvegensis leaf extract; the component B is prepared from the following ingredients in parts by weight: 2 to 4 parts of eucommia ulmoides bark extracts. According to the composition, the verbasci extract, the saw palmetto extract, the picea norvegensis leaf extract and the eucommia ulmoides bark extract are combined to have a synergistic effect, and the composition formed by specific compatibility of the components can prevent androgenetic alopecia from resisting inflammation and bacteria, inhibiting a TRPV3 channel and inhibiting the activity of 5 alpha-reductase; in addition, the plant components are selected, so that the oil-control composition is more suitable for men to use.

Description

Technical Field

[0001] The invention relates to the technical field of cosmetics, in particular to an anti-hair loss composition containing a Norway spruce leaf extract and application thereof. Background Art

[0002] Common types of hair loss include androgenic alopecia (seborrheic alopecia), immune alopecia (neurogenic alopecia, alopecia areata), external stimulation, etc. Among them, androgenic alopecia is an autosomal dominant multi-gene hereditary alopecia that is androgen-dependent. It is often accompanied by oil secretion when it occurs, so it is also called seborrheic alopecia. Its mechanism of occurrence is that the androgen testosterone is converted into more active dihydrotestosterone under the action of 5α-reductase, which enters the cell nucleus to activate specific genes to form specific proteins, regulates the proliferation or differentiation of hair follicle cells, shortens the hair growth period and enters the regression period prematurely. At present, most cosmetics prevent hair loss through anti-hormone effects, such as how to use Polygonum multiflorum extract to reduce the production of androgens, Ganoderma lucidum, Platycladus orientalis leaf extract can inhibit the action of 5α-reductase, etc. However, long-term use of ingredients that inhibit hormone production may affect the hormone balance in the body. In addition, since the scalp is connected to the facial skin, scalp care may affect the growth of facial hair; for men, long-term use of hormone-inhibiting ingredients will also have a certain impact on male secondary sexual characteristics. Therefore, reducing hair loss by inhibiting androgens or 5-α reductase is not suitable for men, and alternative methods need to be explored.

[0003] Studies in recent years have shown that the TRPV3 channel has a great relationship with hair growth. TRPV3 is expressed in large quantities in cells surrounding hair follicles. Activating TRPV3 can inhibit the proliferation of outer root sheath cells of hair follicles, thereby inhibiting hair growth and inducing apoptosis of keratinocytes. TRPV3 channels can be activated under the influence of some activators (such as carvacrol, 2-APB, etc.), and can also be activated at high temperatures (≥33°C). For men, regular exercise or outdoor physical work will increase the temperature of the scalp, making it easier to activate the receptor and aggravate hair loss. Most of the existing anti-hair loss products are aimed at inhibiting androgens or 5α-reductase and are not suitable for men. Therefore, anti-hair loss products suitable for men have become a technical problem that needs to be urgently solved in this field. Summary of the invention

[0004] Based on this, the purpose of the present invention is to overcome the shortcomings of the above-mentioned prior art and provide an anti-hair loss composition and application containing Norway spruce leaf extract. The composition can prevent androgenic alopecia from the effects of anti-inflammatory, antibacterial, inhibiting TRPV3 channels, inhibiting the activity of 5α-reductase, inhibiting lipid secretion from sebaceous gland cells, and achieving the purpose of oil control. Moreover, plant ingredients are selected, which is more suitable for men's use.

[0005] To achieve the above object, the technical solution adopted by the present invention is:

[0006] In a first aspect, the present invention provides an anti-hair loss composition, comprising component A and component B: component A comprises the following ingredients in parts by weight: 1-5 parts of mullein extract, 0.1-1 parts of saw palmetto extract, and 1-5 parts of Norway spruce leaf extract; component B comprises the following ingredients in parts by weight: 2-4 parts of eucommia bark extract.

[0007] The verbascoside contained in the verbascoside extract can inhibit the TRPV3 channel to achieve the purpose of hair loss prevention. In addition, it can also achieve the purpose of anti-inflammatory by inhibiting the TRPV3 channel. Saw palmetto extract can inhibit the activity of 5α-reductase and can be used to prevent androgenic alopecia. Norway spruce leaf extract is rich in polysaccharides, flavonoids, polyphenols and other ingredients, which can inhibit the secretion of lipids by sebaceous gland cells to achieve the purpose of oil control. In addition, inhibiting oil secretion may reduce the occurrence of inflammation to a certain extent. The extract also has anti-inflammatory ingredients such as flavonoids, so it also has a certain skin anti-inflammatory effect. Eucommia bark extract can inhibit the expression of heat-induced MMP-1 gene, reduce epidermal temperature, and has anti-inflammatory effect.

[0008] The present invention has found that when the above-mentioned plant extracts are compounded and used in specific dosages, they can prevent androgenic alopecia from the perspectives of anti-inflammatory, antibacterial, inhibiting TRPV3 channels, inhibiting the activity of 5α-reductase, inhibiting the secretion of lipids by sebaceous gland cells, and achieving the purpose of oil control. Moreover, since all of them are plant ingredients, they are more suitable for men's use.

[0009] As a preferred embodiment of the first aspect, the composition comprises the following ingredients in parts by weight: the composition comprises component A and component B: component A comprises the following ingredients in parts by weight: 1-4 parts of mullein extract, 0.1-0.8 parts of saw palmetto extract, and 1-4 parts of Norway spruce leaf extract; component B comprises the following ingredients in parts by weight: 2-3 parts of eucommia bark extract.

[0010] As a preferred embodiment of the first aspect, the composition includes component A and component B: component A includes the following ingredients in parts by weight: 3 parts of mullein extract, 0.1 parts of saw palmetto extract, and 2 parts of Norway spruce leaf extract; component B includes the following ingredients in parts by weight: 2.5 parts of eucommia bark extract.

[0011] The present invention has found that the mass fractions of mullein extract, saw palmetto extract, Norway spruce leaf and eucommia bark extract in the anti-hair loss composition will not only affect the TNF-α inhibition rate and the antibacterial effect of Malassezia of the composition to a certain extent, thereby affecting the anti-inflammatory, antibacterial and antipruritic effects of the composition; it will also affect the oil control effect of the composition, as well as the inhibitory ability of the composition to 5α-reductase, thereby affecting the oil control and anti-hair loss effects of the composition. When 1-5 parts of mullein extract, 0.1-1 parts of saw palmetto extract, 1-5 parts of Norway spruce leaf extract, and 2-4 parts of eucommia bark extract are further selected, especially 1-4 parts of mullein extract, 0.1-0.8 parts of saw palmetto extract, 1-4 parts of Norway spruce leaf extract, and 2-3 parts of eucommia bark extract, the obtained anti-hair loss composition has better oil control, anti-hair loss, anti-inflammatory, antibacterial and antipruritic effects; when 3 parts of mullein extract, 0.1 parts of saw palmetto extract, 2 parts of Norway spruce leaf extract, and 2.5 parts of eucommia bark extract are added, the overall comprehensive effect of the composition can be enhanced to the greatest extent.

[0012] As a preferred embodiment of the first aspect, in the composition, the mass ratio of component A to component B is: component A: component B = (1.52-3.5): 1. For example, it can be but not limited to 1.52:1, 1.5:1, 2:1, 2.5:1, 3:1, 3.5:1.

[0013] As a preferred embodiment of the first aspect, in the composition, the mass ratio of component A to component B is: component A: component B = (1.7-2.9):1.

[0014] As a preferred embodiment of the first aspect, in the composition, the mass ratio of component A to component B is: component A: component B = 2.04:1.

[0015] The present invention has found that the sum of the mass of the mullein extract, saw palmetto extract, and Norway spruce leaf extract in the anti-hair loss composition and the mass ratio of the Eucommia bark extract will affect the oil control, anti-hair loss, anti-inflammatory, antibacterial and antipruritic effects of the composition to a certain extent. When the mass ratio of (mullein extract + saw palmetto extract + Norway spruce leaf extract) to Eucommia bark extract is further selected to be (1.52-3.5):1, especially (1.7-2.9):1, the oil control, anti-hair loss, anti-inflammatory, antibacterial and antipruritic effects of the composition can be improved. When the mass ratio of (mullein extract + saw palmetto extract + Norway spruce leaf extract) to Eucommia bark extract is 2.04:1, the overall comprehensive effect of the composition can be enhanced to the greatest extent.

[0016] As a preferred embodiment of the first aspect, in the composition A, the mass ratio of the sum of the mass of the mullein extract, the saw palmetto extract and the Norway spruce leaf extract is: mullein extract: (saw palmetto extract + Norway spruce leaf extract) = 1: (1.13-2.3). For example, it can be but not limited to 1: 1.13, 1: 1.18, 1: 2, 1: 2.2, 1: 2.3.

[0017] As a preferred embodiment of the first aspect, in the composition A, the mass ratio of the sum of the mass of the mullein extract, the saw palmetto extract and the Norway spruce leaf extract is: mullein extract: (saw palmetto extract + Norway spruce leaf extract) = 1: (1.17-2.1).

[0018] As a preferred embodiment of the first aspect, in the composition A, the mass ratio of the sum of the mass of the mullein extract, the saw palmetto extract and the Norway spruce leaf extract is: mullein extract: (saw palmetto extract + Norway spruce leaf extract) = 1:1.13.

[0019] The present invention has found that the mass ratio of the sum of the mass of the mullein extract, the saw palmetto extract and the Norway spruce leaf extract in the men's anti-hair loss composition will affect the anti-inflammatory, antibacterial, antipruritic and oil-control effects of the composition to a certain extent. When the mass ratio of the mullein extract to (saw palmetto extract + Norway spruce leaf extract) is further selected to be 1: (1.13-2.3), especially 1: (1.17-2.1), the anti-inflammatory, antibacterial, antipruritic and oil-control effects of the composition can be improved. When the mass ratio of the mullein extract to (saw palmetto extract + Norway spruce leaf extract) is 1: 1.13, the overall comprehensive effect of the composition can be enhanced to the greatest extent.

[0020] As a preferred embodiment of the first aspect, the method for preparing the mullein extract comprises the following steps: taking dried leaves of mullein, drying them, crushing them, adding them to an aqueous solution of a low eutectic solvent, ultrasonically extracting them, collecting the products, centrifuging them, taking the supernatant, and obtaining the mullein extract; wherein the low eutectic solvent is composed of butanediol and malic acid.

[0021] As a preferred embodiment of the first aspect, the method for preparing the mullein extract satisfies at least one of the following requirements:

[0022] (a) the molar ratio of butanediol to malic acid is: butanediol: malic acid = 1: (1-4);

[0023] (b) the mass concentration of the deep eutectic solvent is 20 to 50%;

[0024] (c) the temperature of the ultrasonic extraction is 50-60°C;

[0025] (d) the power of the ultrasonic extraction is 60-70W;

[0026] (e) the ultrasonic extraction time is 30-60 min;

[0027] (f) The material-liquid ratio of the ultrasonic extraction is 1 g: (20-30) mL.

[0028] The present invention finds that different extraction methods have an impact on the mullein extract, and affect the synergistic effect of the mullein extract and other components, affecting the anti-inflammatory, antibacterial and antipruritic as well as oil control and anti-hair loss effects of the composition, thereby affecting the overall comprehensive effect of the composition.

[0029] In a second aspect, the present invention provides use of the anti-hair loss composition as described in the first aspect in the preparation of an anti-hair loss product.

[0030] In a third aspect, the present invention provides an oil control and anti-hair loss product, wherein the oil control and anti-hair loss product comprises the anti-hair loss composition for men described in the first aspect.

[0031] Compared with the prior art, the beneficial effects of the present invention are:

[0032] The present invention finds that mullein extract, saw palmetto extract, Norway spruce leaf, and eucommia bark extract have a synergistic effect from numerous plant extracts. The composition formed by specifically combining them can prevent androgenic alopecia from the effects of anti-inflammatory, antibacterial, inhibiting TRPV3 channels, inhibiting the activity of 5α-reductase, and inhibiting lipid secretion by sebaceous gland cells, thereby achieving the purposes of anti-inflammatory, antibacterial, antipruritic, and oil control and hair loss prevention. Moreover, plant ingredients are selected, which is more suitable for men. DETAILED DESCRIPTION

[0033] In order to better illustrate the purpose, technical scheme and advantages of the present invention, the present invention will be further described below in conjunction with specific embodiments. Its purpose is to understand the content of the present invention in detail, rather than to limit the present invention. All other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention. Unless otherwise specified, the experimental reagents and instruments designed for the implementation and comparative examples of the present invention are all commonly used ordinary reagents and instruments, which can be obtained from commercial channels. In the implementation and comparative examples, the experimental methods used are conventional methods unless otherwise specified; and unless otherwise specified, the raw materials used in parallel experiments are from the same batch of raw materials. Further description of the raw materials used in the present invention:

[0034] Saw palmetto extract was purchased from Shaanxi Hanqing Biotechnology Co., Ltd., product name: Saw palmetto extract;

[0035] Norway spruce leaf extract was purchased from Canada Norture Company (Norture Lights Cell Science Inc.), product name: Norway spruce (PICEA EXCELSA) leaf extract;

[0036] Rehmannia root extract was purchased from Shaanxi Lvshengyuan Biological Products Manufacturing Co., Ltd., product name: Rehmannia root extract;

[0037] Platycladus orientalis leaf extract was purchased from Shanxi Yirun Biotechnology Co., Ltd., product name: Platycladus orientalis leaf extract;

[0038] Tremella fuciformis extract was purchased from Hubei Xinyuhong Biopharmaceutical Technology Co., Ltd., product name: Tremella fuciformis extract;

[0039] Eucommia ulmoides extract was purchased from Shaanxi Guben Biotechnology Co., Ltd., product name: Eucommia ulmoides extract.

[0040] Preparation of Eucommia bark extract: Take commercially available Eucommia bark, dry and crush it, add 5 times the amount of 50% ethanol solution, stir at 50°C for 24 hours, filter, and concentrate to half of the original volume at 60°C to obtain Eucommia bark extract.

[0041] The preparation method of mullein extract-1 is as follows:

[0042] (1) Prepare a low eutectic solvent:

[0043] A certain amount of a low eutectic solvent composed of butanediol and malic acid in a molar ratio of 1:2 is taken and diluted with deionized water to form an aqueous solution of the low eutectic solvent with a mass concentration of 20%;

[0044] (2) Preparation of Verbascum extract:

[0045] The dried leaves of mullein are collected, ground and crushed, and then passed through a 40-mesh sieve. 1 g is taken and added to the aqueous solution of the above-mentioned low eutectic solvent, and the solid-liquid ratio is 1 g: 20 mL; an ultrasonic extraction method is used, the ultrasonic temperature is 50°C, the power is 60 W, the extraction time is 30 min, the product is collected and centrifuged, and the supernatant is taken to obtain the mullein extract-1.

[0046] The preparation method of mullein extract-2 is as follows:

[0047] (1) Prepare a low eutectic solvent:

[0048] A certain amount of a low eutectic solvent composed of butanediol and malic acid in a molar ratio of 1:4 is taken and diluted with deionized water to form an aqueous solution of the low eutectic solvent with a mass concentration of 30%;

[0049] (2) Preparation of Verbascum extract:

[0050] The dried leaves of mullein are collected, ground and crushed, and then passed through a 50-mesh sieve. 1 g is taken and added to the aqueous solution of the above-mentioned low eutectic solvent, and the solid-liquid ratio is 1 g: 25 mL; an ultrasonic extraction method is used, the ultrasonic temperature is 55°C, the power is 65 W, the extraction time is 40 min, the product is collected and centrifuged, and the supernatant is taken to obtain the mullein extract-2.

[0051] The preparation method of mullein extract-3 is as follows:

[0052] (1) Prepare a low eutectic solvent:

[0053] A certain amount of a low eutectic solvent composed of butanediol and malic acid in a molar ratio of 1:1 is taken and diluted with deionized water to form an aqueous solution of the low eutectic solvent with a mass concentration of 50%;

[0054] (2) Preparation of Verbascum extract:

[0055] The dried leaves of mullein are collected, ground and crushed, and then passed through a 70-mesh sieve. 1 g is taken and added to the aqueous solution of the above-mentioned low eutectic solvent, and the solid-liquid ratio is 1 g:30 mL; ultrasonic extraction method is used, the ultrasonic temperature is 60°C, the power is 70 W, the extraction time is 60 min, the product is collected and centrifuged, and the supernatant is taken to obtain the mullein extract-3.

[0056] The preparation method of mullein extract-4 is as follows:

[0057] The dried leaves of mullein are collected, cleaned and placed in a 50°C constant temperature oven to dry to constant weight, ground and crushed, and passed through an 80-mesh sieve, 1 g is taken, and an aqueous solution with a mass percentage of ethanol of 75% is added, with a solid-liquid ratio of 1 g: 30 mL; an ultrasonic extraction method is used, with an ultrasonic temperature of 50°C, a power of 160 W, an extraction time of 20 min, the product is collected and centrifuged, and the supernatant is taken to obtain the mullein extract-4.

[0058] The preparation method of mullein extract-5 is as follows:

[0059] The dried leaves of mullein are collected, cleaned, placed in a 50°C constant temperature oven and dried to constant weight, ground and crushed, and passed through an 80-mesh sieve to obtain the dried leaf powder of mullein; 1 g of the dried leaf powder of mullein is added with 20 mL of 70% ethanol by volume, and refluxed at 80°C for 1 hour to obtain the mullein extract-5.

[0060] The preparation method of the anti-hair loss composition for men suitable for sensitive skin of the present invention is as follows: weigh the components in parts by weight as described in Table 1, add water to 20 g, stir and mix evenly, and ultrasonicate for 10 minutes to obtain the anti-hair loss composition for men.

[0061] Examples and Comparative Examples

[0062] The present application provides an anti-hair loss composition suitable for men, and the components and weight portions of the composition are shown in Table 1:

[0063] Table 1:

[0064]

[0065]

[0066] Effect example

[0067] 1. Anti-inflammatory ability of the composition:

[0068] Plating: RAW264.7 cells in the logarithmic growth phase were inoculated in 24-well plates at a cell density of 2×10 5 / mL, 0.5mL per well, mark the top cover of the cell plate after laying the plate, and place it in a carbon dioxide incubator for incubation for 24h;

[0069] Sample treatment: After incubating the cells for 24 hours, the supernatant was removed and 0.5 mL of the following samples were added respectively: ① blank control group: DMEM medium; ② LPS inflammation model group: LPS (1 μg / mL); ③ positive control group: 0.001% dexamethasone + LPS (1 μg / mL); ④ experimental group: 2% embodiment or comparative example composition + LPS (1 μg / mL);

[0070] Detection of inflammatory factors in cell supernatant: After the sample is treated with cells for 24 hours, the supernatant is aspirated into a centrifuge tube with a pipette, centrifuged at 1000r / min for 5 minutes, the precipitate is discarded and the supernatant is aspirated, and the change in the amount of inflammatory factors in the supernatant is detected using the Xinbosheng TNF-α inflammatory factor detection kit; the inhibition rate of TNF-α is calculated, TNF-α inhibition rate % = (TNF-α amount in the model group - TNF-α amount in the experimental group) / TNF-α amount in the model group × 100%; wherein, the higher the TNF-α inhibition rate %, the stronger the anti-inflammatory ability. The calculation results are shown in Table 2:

[0071] 2. Inhibitory effect of the composition on 5α-reductase

[0072] Sample tube: Add 1 mL of sample solution, 1 mL of enzyme solution, 1 mL of NADPH solution and 1 mL of testosterone solution to the test tube, shake gently, and use a pipette to take 200 μL to a 96-well ELISA plate. Make three parallels for each sample, put it into the ELISA reader for detection, and measure the absorbance at 340 nm, which is the first measurement value A sample 0. After incubating at 37°C for 20 minutes, put it into the ELISA reader for detection, and measure the absorbance at 340 nm, which is the second measurement value A sample 20.

[0073] Enzyme tube: Add 1mL PBS solution, 1mL enzyme solution, 1mL NADPH solution and 1mL testosterone solution into the test tube, shake gently, use a pipette to take 200μL into a 96-well ELISA plate, make three parallels for each sample, put it into the ELISA reader for detection, and measure the absorbance at 340nm, which is the first measurement value A 酶0 After the sample is incubated at 37°C for 20 minutes, it is placed in an ELISA reader for detection. The absorbance value at 340 nm is the second measurement value A. 酶20 The inhibition rate of the enzyme was calculated according to the following formula, and the calculated results are shown in Table 2;

[0074]

[0075] Among them, the higher the 5α-reductase inhibition rate, the stronger the anti-hair loss ability.

[0076] Table 2:

[0077] Group TNF-α inhibition rate % Group 5α-reductase inhibition rate % Positive control group 45.7％ Positive control group 78.3％ Example 1 31.0％ Example 1 38.8％ Example 2 33.2％ Example 2 44.1％ Example 3 33.6％ Example 3 40.5％ Example 4 33.1％ Example 4 43.3％ Example 5 37.3％ Example 5 46.5％ Example 6 35.2％ Example 6 47.3％ Example 7 39.1％ Example 7 49.4％ Example 8 37.7％ Example 8 49.7％ Example 9 44.2％ Example 9 56.3％ Example 10 41.1％ Example 10 52.5％ Embodiment 11 41.7％ Embodiment 11 51.1％ Example 12 40.8％ Example 12 51.3％ Comparative Example 1 26.2％ Comparative Example 1 25.8％ Comparative Example 2 30.1％ Comparative Example 2 36.3％ Comparative Example 3 22.5％ Comparative Example 3 30.5％ Comparative Example 4 22.7％ Comparative Example 4 14.8％ Comparative Example 5 20.1％ Comparative Example 5 27.7％ Comparative Example 6 20.4％ Comparative Example 6 27.9％ Comparative Example 7 25.1％ Comparative Example 7 26.8％ Comparative Example 8 24.4％ Comparative Example 8 16.8％ Comparative Example 9 22.9％ Comparative Example 9 25.9％ Comparative Example 10 22.8％ Comparative Example 10 25.3％

[0078] It can be seen from Table 2 that the anti-hair loss composition provided by the present invention can significantly improve the TNF-α inhibition rate and 5α-reductase inhibition rate, which shows that the combination of the present invention has strong anti-inflammatory and androgen-reducing effects, achieving the purpose of anti-hair loss.

[0079] It can be seen from Examples 1-8 and Comparative Examples 1-2 that the weight parts and mass ratios of the components in the anti-hair loss composition will affect the anti-inflammatory and anti-hair loss effects of the composition.

[0080] It can be seen from Examples 1-5 that the mass ratio of the components in the composition affects the anti-inflammatory and anti-hair loss capabilities of the composition. When the mass ratio of (Verbascum extract + Saw Palmetto extract + Norway spruce leaf extract) to Eucommia bark extract is (1.52-3.5):1, the resulting composition has a strong anti-inflammatory and anti-hair loss effect, and when it is further (1.7-2.9):1, the resulting composition has a better anti-inflammatory and anti-hair loss effect, and when the mass ratio is 2.04:1 (Example 5), the effect is the best.

[0081] The present invention studies the effect of the mass ratio of the sum of the mass of the mullein extract and the mass of the saw palmetto extract and the Norway spruce leaf extract in the composition on the inhibition of TNF-α by the composition through Examples 6-8. It can be seen from Examples 6-8 that the mass ratio of the sum of the mass of the mullein extract and the mass of the saw palmetto extract and the Norway spruce leaf extract affects the effect of the composition on the inhibition of TNF-α, thereby affecting the anti-inflammatory effect of the composition. When the mass ratio of the mullein extract to (saw palmetto extract + Norway spruce leaf extract) is 1: (1.13-2.3), the ability of the composition to inhibit TNF-α can be improved, especially when it is 1: (1.17-2.1), the effect of the obtained composition is better, and when the mass ratio is 1: 1.13 (Example 7), the effect is best.

[0082] The present invention studies the effect of different extraction methods of the extracts of Verbascum on the ability of the composition to inhibit TNF-α through Examples 9-12. It can be seen from Examples 9-12 that the extraction method of the extract of Verbascum has a certain effect on the effect of inhibiting TNF-α, thereby affecting the anti-inflammatory effect of the composition. When the extract of Verbascum-2 is used, the anti-inflammatory effect of the composition obtained is the best (Example 9).

[0083] The TNF-α inhibition rate of Comparative Example 1-2 is 26-30%, and the 5α-reductase inhibition rate is 55-60%. This is because the mass ratios of the components in the compositions of Comparative Example 1-2 are not within the scope of the present invention, which affects the synergistic effect between the components and reduces the efficacy of the composition.

[0084] The TNF-α inhibition rate of Comparative Examples 3-6 is 20-22%, and the 5α-reductase inhibition rate is 40-60%. This is because the lack of one of the components of the present invention in the composition of Comparative Examples 3-6 affects the synergistic effect between the components and reduces the efficacy of the composition.

[0085] The TNF-α inhibition rate of Comparative Examples 7-10 is 22-25%, and the 5α-reductase inhibition rate is 40-56%. This is because the composition of Comparative Examples 7-10 replaces one of the components in the combination group of the present invention with other plant extracts, which does not produce a synergistic effect with the specific components selected by the present invention, and also affects the synergistic effect between other components of the present invention, thereby reducing the efficacy of the composition.

[0086] 3. Test of the antibacterial effect of the composition on Malassezia

[0087] Malassezia (ATCC 44344) is lipophilic, and the colonies will float on the surface of the liquid. The data is read using the direct method, specifically: a single colony of Malassezia (ATCC 44344) is placed in 3 mL of Malassezia culture medium (SDA agar medium + 1% Tween 40 + 0.6% ox bile salt + 0.001% chloramphenicol) and cultured at 37°C, 200 rpm for 48 hours. The cultured Malassezia is diluted to 10 5 cfu / ml. The compositions in the examples and comparative examples were diluted to concentrations of 0.1%, 0.2%, 0.5%, 1%, 2%, and 5%, and 100 μL of each was placed in a 96-well plate. Each concentration was repeated 3 times, and 100 μL of Malassezia liquid was added to each well to observe whether it was clear. If it was clear, it had an antibacterial effect. The test results are shown in Table 3 below:

[0088] Table 3:

[0089]

[0090]

[0091] It can be seen from Table 3 that the anti-hair loss composition provided by the present invention can significantly inhibit Malassezia, which indicates that the composition of the present invention has a strong antibacterial and antipruritic effect.

[0092] It can be seen from Examples 1-8 and Comparative Examples 1-2 that the weight parts and mass ratios of the components in the anti-hair loss composition will affect the antibacterial and antipruritic effects of the composition.

[0093] It can be seen from Examples 1-5 that the mass ratio of the components in the composition affects the antibacterial ability of the composition. When the mass ratio of (Verbascum extract + Saw Palmetto extract + Norway spruce leaf extract) to Eucommia bark extract is (1.52-3.5):1, the obtained composition has a strong antibacterial and antipruritic effect, and when it is further (1.7-2.9):1, the obtained composition has a better antibacterial and antipruritic effect, and when the mass ratio is 2.04:1 (Example 5), the effect is the best.

[0094] The present invention studies the influence of the mass ratio of the sum of the mass of the mullein extract, the saw palmetto extract and the Norway spruce leaf extract in the composition on the antibacterial effect of the composition through Examples 6-8. It can be seen from Examples 6-8 that the mass ratio of the sum of the mass of the mullein extract, the saw palmetto extract and the Norway spruce leaf extract affects the antibacterial and antipruritic effect of the composition. When the mass ratio of the mullein extract to (saw palmetto extract + Norway spruce leaf extract) is 1: (1.13-2.3), the antibacterial and antipruritic ability of the composition can be improved, especially when it is 1: (1.17-2.1), the effect of the obtained composition is better. When the mass ratio is 1: 1.13 (Example 7), the effect is best.

[0095] The present invention studies the effect of different extraction methods of the extracts of Verbascum on the antibacterial ability of the composition through Examples 9-12. It can be seen from Examples 9-12 that the extraction method of the extracts of Verbascum has a certain effect on the antibacterial effect, thereby affecting the antibacterial and antipruritic effect of the composition. When the extract of Verbascum-2 is used, the antibacterial and antipruritic effect of the composition obtained is the best (Example 9).

[0096] The antibacterial effect of Comparative Example 1-2 is relatively poor. This is because the mass ratios of the components in the compositions of Comparative Example 1-2 are not within the scope of the present invention, which affects the synergistic effect between the components and reduces the efficacy of the composition.

[0097] The antibacterial effect of Comparative Examples 3-6 is relatively poor. This is because the lack of one of the components of the present invention in the composition of Comparative Examples 3-6 affects the synergistic effect between the components and reduces the efficacy of the composition.

[0098] The antibacterial effect of comparative examples 7-10 is poor. This is because the comparative examples 7-10 replace one of the components in the combination group of the present invention with other plant extracts, which do not produce a synergistic effect with the specific components selected by the present invention, and also affect the synergistic effect between other components of the present invention, thereby reducing the efficacy of the composition.

[0099] Application Examples

[0100] The application examples of the present invention and the comparative application examples provide a men's anti-hair loss product, and the components (mass percentage) of the anti-hair loss product are shown in Table 4; wherein the men's anti-hair loss compositions used in application examples 1-12 are respectively the men's anti-hair loss compositions prepared in embodiments 1-12; application examples 13-14 use the composition prepared in embodiment 9; the men's anti-hair loss compositions used in comparative application examples 1-10 are respectively the men's anti-hair loss compositions prepared in comparative examples 1-10; in addition, a blank application example is provided, and the only difference between the blank application example and application example 1 is that the men's anti-hair loss composition is not added;

[0101] Table 4:

[0102]

[0103]

[0104] This embodiment provides a method for preparing shampoo, and the preparation method comprises the following steps:

[0105] (1) Add polyquaternium-10 to deionized water and stir to disperse, and then add other materials of phase A after uniform dispersion, heat to 85° C., stir to dissolve uniformly, and then keep warm for standby use to obtain prefabricated phase A;

[0106] (2) Add the ingredients of phase B at 80-85°C, stir thoroughly to dissolve evenly until transparent, and keep warm to remove foam;

[0107] (3) Cool down to below 45°C, add the raw materials of phase C one by one, and stir evenly;

[0108] (4) Continue to add the raw materials of phase D and mix well to obtain the men's anti-hair loss shampoo.

[0109] Test Example 1: Oil control effect test of anti-hair loss products:

[0110] A number of male volunteers aged 18-45 were selected. The subjects sat quietly in a constant temperature and humidity room with a temperature of 21±1°C and a humidity of 50±10% for 30 minutes. The initial oil content was measured using the scalp oil tester Meibometer MB560, and the test site was the top of the head. The subjects were randomly divided into 25 groups, with 5 people in each group. The anti-hair loss product provided by the present invention was randomly used, and the test was carried out at 8 weeks and 12 weeks. The improvement of the oil content of each volunteer at 8 and 12 weeks was analyzed. The improvement was represented by the oil reduction rate, which was calculated as follows:

[0111] Reduction rate relative to the initial value % = |(X 使用后 -X 使用前 ) / X 使用前 ×100%|; The greater the reduction rate % relative to the initial value, the stronger the anti-slip ability. The calculated data are shown in Table 5;

[0112] Table 5:

[0113]

[0114]

[0115] It can be seen from Table 5 that the anti-hair loss product provided by the present invention can significantly reduce oil secretion, which indicates that the composition of the present invention has a strong oil control and anti-hair loss effect.

[0116] It can be seen from Application Examples 1-8 and Comparative Application Examples 1-2 that the weight parts and mass ratios of the components in the anti-hair loss composition will affect the oil control and anti-hair loss efficacy of the composition.

[0117] It can be seen from Application Examples 1-5 that the mass ratio of the components in the composition affects the antibacterial ability of the composition. When the mass ratio of (Mullein extract + Saw Palmetto extract + Norway Spruce leaf extract) to Eucommia bark extract is (1.52-3.5):1, the obtained composition has a strong oil control and anti-hair loss effect. When it is further (1.7-2.9):1, the obtained composition has a better oil control and anti-hair loss effect. When the mass ratio is 2.04:1 (Application Example 5), the effect is the best.

[0118] The present invention studies the influence of the mass ratio of the sum of the mass of the mullein extract, the saw palmetto extract and the Norway spruce leaf extract in the composition on the oil control and anti-hair loss effect of the composition through application examples 6-8. It can be seen that the mass ratio of the sum of the mass of the mullein extract, the saw palmetto extract and the Norway spruce leaf extract affects the oil control and anti-hair loss effect of the composition. When the mass ratio of the mullein extract to (saw palmetto extract + Norway spruce leaf extract) is 1: (1.13-2.3), the oil control and anti-hair loss ability of the composition can be improved, especially when it is 1: (1.17-2.1), the effect of the obtained composition is better. When the mass ratio is 1: 1.13 (application example 7), the effect is best.

[0119] The present invention studies the effects of the mullein extracts obtained by different extraction methods on the oil control and anti-hair loss ability of the composition through application examples 9-12. It can be seen that the extraction method of the mullein extract has a certain effect on oil secretion, thereby affecting the oil control and anti-hair loss effect of the composition. When the mullein extract-2 is used, the oil control and anti-hair loss effect of the composition obtained is the best (application example 9).

[0120] The oil reduction rate of Comparative Examples 1-2 is 16-18%. This is because the mass ratios of the components in the compositions of Comparative Examples 1-2 are not within the scope of the present invention, which affects the synergistic effect between the components and reduces the efficacy of the composition.

[0121] The oil reduction rate of Comparative Examples 3-6 is 14-15%. This is because the lack of one of the components of the present invention in the composition of Comparative Examples 3-6 affects the synergistic effect between the components and reduces the efficacy of the composition.

[0122] The oil reduction rate of Comparative Examples 7-10 is 15-16%. This is because the compositions of Comparative Examples 7-10 replace one of the components in the combination group of the present invention with other plant extracts, which do not produce a synergistic effect with the specific components selected by the present invention, and also affect the synergistic effect between other components of the present invention, thereby reducing the efficacy of the composition.

[0123] Test Example 2: Test on the anti-hair loss effect of anti-hair loss products on human body

[0124] A number of male volunteers aged 18-45 years old, all with different degrees of hair loss problems, were selected and divided into 25 groups with 5 people in each group. The subjects used the anti-hair loss products of the above application examples or comparative application examples once a day. After 8 and 12 weeks of use, the hair loss was counted. The 60-times combing method was used to count the number of hair lost in 60 combings. The number of hair loss was counted before using the product and after 8 and 12 weeks of use. The subjects did not wash their hair within 48±4 hours before each visit and did not comb their hair on the day of the visit. The hair loss reduction rate was calculated, hair loss reduction rate / %=(number of hair loss roots at 0 weeks-number of hair loss roots at 8 / 12 weeks) / number of hair loss roots at 0 weeks; the calculated data are shown in Table 6:

[0125] Table 6

[0126]

[0127]

[0128] It can be seen from Table 6 that the anti-hair loss product provided by the present invention has an excellent anti-hair loss effect. Specifically, at the 12th week, the obtained hair loss reduction rate was above 40%.

[0129] It can be seen from Application Examples 1-8 and Comparative Application Examples 1-2 that the weight parts and mass ratios of the components in the anti-hair loss composition will affect the oil control and anti-hair loss efficacy of the composition.

[0130] It can be seen from Application Examples 1-5 that the mass ratio of the components in the composition will affect the anti-hair loss ability of the composition. When the mass ratio of (Verbascum extract + Saw Palmetto extract + Norway spruce leaf extract) to Eucommia bark extract is (1.52-3.5):1, the obtained composition has a strong anti-hair loss effect, and when it is further (1.7-2.9):1, the obtained composition has a better anti-hair loss effect, and when the mass ratio is 2.04:1 (Application Example 5), the effect is the best.

[0131] The present invention studies the influence of the mass ratio of the verbabasin extract, saw palmetto extract and Norway spruce leaf extract in the composition on the anti-hair loss effect of the composition through application examples 6-8. It can be seen that the mass ratio of the total mass of the verbabasin extract, saw palmetto extract and Norway spruce leaf extract affects the anti-hair loss effect of the composition. When the mass ratio of the verbabasin extract to (saw palmetto extract + Norway spruce leaf extract) is 1: (1.13-2.3), the anti-hair loss ability of the composition can be improved, especially when it is 1: (1.17-2.1), the effect of the obtained composition is better. When its mass ratio is 1: 1.13 (application example 7), its effect reaches the best.

[0132] The present invention studies the effect of different extraction methods of the mullein extract on the anti-hair loss ability of the composition through application examples 9-12. It can be seen that the extraction method of the mullein extract affects the anti-hair loss effect of the composition. When the mullein extract-2 is used, the anti-hair loss effect of the composition obtained is the best (application example 9).

[0133] The hair loss reduction rate of Comparative Examples 1-2 is 36-38%. This is because the mass ratios of the components in the compositions of Comparative Examples 1-2 are not within the scope of the present invention, which affects the synergistic effect between the components and reduces the efficacy of the composition.

[0134] The hair loss reduction rate of Comparative Example 3-6 is 33%. This is because the lack of one of the components of the present invention in the composition of Comparative Example 3-6 affects the synergistic effect between the components and reduces the efficacy of the composition.

[0135] The hair loss reduction rate of comparative examples 7-10 is 34-36%. This is because the comparative examples 7-10 compositions replace one of the components in the combination group of the present invention with other plant extracts, which do not produce a synergistic effect with the specific components selected by the present invention, and also affect the synergistic effect between other components of the present invention, thereby reducing the efficacy of the composition.

[0136] Test Example 3: Test on use of anti-hair loss products by male consumers:

[0137] The effect example of the present invention verifies the applicability and anti-hair loss soothing effect of the anti-hair loss product prepared in Application Example 1 to male consumers, and specifically includes the following steps:

[0138] 30 male subjects were selected to participate in the test. The product was distributed to them. Each subject applied an appropriate amount of the product daily for a 28-day applicability test. Consumers completed a questionnaire survey on the improvement of indicators such as anti-hair loss effect, soothing effect, oil control effect, and irritation. See Table 7 for details:

[0139] Table 7:

[0140]

[0141] It can be seen from Table 7 that the anti-hair loss product of the present invention with the anti-hair loss composition added has the effects of anti-hair loss, soothing, and relieving itching, and can make the subjects experience a refreshing and comfortable feeling. At the same time, the anti-hair loss product of the present invention is mild and non-irritating, and is suitable for men.

[0142] Finally, it should be noted that the above embodiments are only used to illustrate the technical solution of the present invention rather than to limit the scope of protection of the present invention. Although the present invention has been described in detail with reference to the preferred embodiments, those skilled in the art should understand that the technical solution of the present invention can be modified or replaced by equivalents without departing from the essence and scope of the technical solution of the present invention.

Claims

1. An anti-shedding composition, characterized in that The composition comprises component A and component B: The component A comprises the following ingredients in parts by weight: 1-5 parts of mullein extract, 0.1-1 parts of saw palmetto extract, and 1-5 parts of Norway spruce leaf extract; The component B comprises the following ingredients in parts by weight: 2-4 parts of Eucommia bark extract.

2. The anti-shedding composition according to claim 1, characterized in that The composition comprises component A and component B: The component A comprises the following ingredients in parts by weight: 1-4 parts of mullein extract, 0.1-0.8 parts of saw palmetto extract, and 1-4 parts of Norway spruce leaf extract; The component B comprises the following ingredients in parts by weight: 2-3 parts of Eucommia bark extract.

3. The anti-shedding composition according to claim 1, characterized in that In the composition, the mass ratio of component A to component B is: component A: component B = (1.52-3.5):

1.

4. The anti-shedding composition according to claim 3, characterized in that In the composition, the mass ratio of component A to component B is: component A: component B = (1.7-2.9):

1.

5. The anti-shedding composition according to claim 1, characterized in that In the composition A, the mass ratio of the sum of the mass of the mullein extract, the saw palmetto extract and the Norway spruce leaf extract is: mullein extract: (saw palmetto extract + Norway spruce leaf extract) = 1: (1.13-2.3).

6. The anti-shedding composition according to claim 5, characterized in that In the composition A, the mass ratio of the sum of the mass of the mullein extract, the saw palmetto extract and the Norway spruce leaf extract is: mullein extract: (saw palmetto extract + Norway spruce leaf extract) = 1: (1.17-2.1).

7. The anti-shedding composition according to any one of claims 1 to 6, characterized in that The preparation method of the mullein extract comprises the following steps: The dried leaves of mullein are dried, crushed, added to an aqueous solution of a low eutectic solvent for ultrasonic extraction, the product is collected and centrifuged, and the supernatant is taken to obtain the mullein extract; wherein the low eutectic solvent consists of butanediol and malic acid.

8. The anti-shedding composition according to claim 7, characterized in that Satisfy at least one of the following: (a) the molar ratio of butanediol to malic acid is: butanediol: malic acid = 1: (1-4); (b) the mass concentration of the deep eutectic solvent in the aqueous solution of the deep eutectic solvent is 20 to 50%; (c) the temperature of the ultrasonic extraction is 50-60°C; (d) the power of the ultrasonic extraction is 60-70W; (e) the ultrasonic extraction time is 30-60 min; (f) The solid-liquid ratio of the ultrasonic extraction is 1 g: (20-30) mL.

9. Use of the anti-hair loss composition according to any one of claims 1 to 8 in the preparation of an anti-hair loss product.

10. An oil control and anti-hair loss product, characterized in that: The oil control and anti-hair loss product comprises the anti-hair loss composition according to any one of claims 1 to 8.

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