A flowable concentrate and its use in the control of potato cyst nematode and late blight

Abstract

The application discloses a kind of suspension concentrate and its application in the prevention and treatment of potato cyst nematode disease and late blight, belong to the technical field of pesticide.The application discloses a kind of suspension concentrate, and the effective component is fluazamamide, precise thiamethoxam and fludioxonil.The suspension concentrate of the application has good film-forming property, uniform coating and does not affect the emergence rate of seed potato.Compared with each single agent of the pesticide, the suspension concentrate of the application can not only prevent and treat potato cyst nematode disease, but also has certain prevention and treatment effect on potato late blight, has synergistic effect.The suspension concentrate of the application is used for the treatment of potato seed potato, saves time and labor, and can delay the drug resistance of disease, has good application prospect.

Description

Technical Field

[0001] This invention relates to the field of pesticide technology, and more specifically to a suspension seed dressing agent and its application in the control of potato cyst nematode disease and late blight. Background Technology

[0002] Potato late blight (Phytophthora infestans) is a crop disease caused by oomycetes, posing a significant threat to my country's potato industry. This disease primarily affects the stems, leaves, and tubers of potatoes, leading to yield losses of 10-20%; in years of severe outbreaks, this figure can reach 50-70%, resulting in substantial yield reductions and, in severe cases, total crop failure. Potato cyst nematode disease is another important pest affecting potato cultivation, mainly including the potato golden nematode (Globodera rostochiensis) and the potato white nematode (G. pallida). This disease was first discovered in Europe and is now widespread in countries such as the UK, Netherlands, Germany, France, and Belgium. Statistics show that approximately 64% of potato fields in the EU are affected by potato cyst nematode disease, typically causing a 20% yield reduction, and in severe cases, yield losses of 80-90%, or even total crop failure.

[0003] Fluopyram is a broad-spectrum fungicide belonging to the pyrazolamide class of compounds. It was later discovered to be effective against plant nematode diseases. It works by inhibiting complex II (succinate dehydrogenase) in the mitochondrial respiratory chain, thereby preventing energy metabolism in fungal cells and achieving a fungicidal / nematode-killing effect. Fluopyram has excellent systemic and translocative properties, allowing it to be rapidly absorbed by plants and translocated within the plant body, effectively controlling diseases on various crops.

[0004] Metalaxyl-M is a broad-spectrum, highly effective fungicide belonging to the phenylamide class of compounds. It is primarily used to control various crop diseases caused by oomycete pathogens, particularly downy mildew and late blight, demonstrating significant control efficacy. Metalaxyl-M works by inhibiting RNA polymerase within pathogen cells, thus preventing nucleic acid synthesis and achieving its fungicidal effect.

[0005] Fludioxonil is a broad-spectrum and highly effective fungicide belonging to the benzimidazole class of compounds. It is primarily used to control various crop diseases caused by ascomycetes, basidiomycetes, and deuteromycetes, with particularly significant effects on diseases during storage and transportation. Fludioxonil achieves its fungicidal effect by interfering with chitin synthesis in the cell walls of pathogens, thus inhibiting their normal development.

[0006] Seed dressing agents are an ideal preparation for controlling root diseases in crops. Treating potato seed tubers with seed dressing agents is a common agronomic practice that can effectively kill pathogens attached to the surface of seed tubers and reduce the risk of soil-borne diseases, especially for some difficult-to-cure diseases such as nematode disease, late blight, ring rot, black scurf, and wilt.

[0007] Therefore, providing a suspension seed coating agent and its application in the prevention and control of potato cyst nematode disease and late blight is a problem that urgently needs to be solved by those skilled in the art. Summary of the Invention

[0008] In view of this, the present invention provides a suspension seed dressing agent and its application in the control of potato cyst nematode disease and late blight. The suspension seed dressing agent is a compound seed dressing agent containing fluopyram, metalaxyl, and fludioxonil, which can control both potato cyst nematode disease and potato late blight.

[0009] To achieve the above objectives, the present invention adopts the following technical solution:

[0010] A suspension seed coating agent, by weight percentage, contains the following components: fluopyram 5-40%, metalaxyl 1.8-3.5%, fludioxonil 1.2-2.5%, adjuvants 7.6-33%, and water to 100%.

[0011] Furthermore, the additive contains the following components: 2-5% dispersant, 1-6% wetting agent, 0.5-3% film-forming agent, 0.1-3% stabilizer, 1-5% antifreeze, 1-5% defoamer, 1-3% preservative, and 1-3% colorant.

[0012] Furthermore, the dispersant is a polyoxyethylene polyoxypropylene ether block copolymer, nonylphenol polyoxyethylene ether, fatty alcohol polyoxyethylene ether, alkylphenol polyoxyethylene ether, octylphenol polyoxyethylene ether, calcium lignosulfonate, polycarboxylate, sodium lauryl alcohol polyoxyethylene ether sulfate, triphenylethylphenol polyoxyethylene ether, or sodium lignosulfonate.

[0013] The wetting agent is sodium dodecylbenzenesulfonate, sodium dodecyl sulfate, or a separating powder;

[0014] The film-forming agent is gum arabic, pectin, methylcellulose, ethylcellulose, polyvinyl alcohol, polyethylene glycol, animal glue, hydroxypropyl cellulose, sodium alginate, or polyvinylpyrrolidone.

[0015] The stabilizer is xanthan gum, diatomaceous earth, sodium carboxymethyl cellulose, magnesium aluminum silicate, bentonite, cellulose ether and its derivatives or silica.

[0016] The antifreeze is ethylene glycol, propylene glycol, or glycerol;

[0017] The defoamer is an organosilicone defoamer;

[0018] The preservatives mentioned are potassium sorbate, industrial-grade formaldehyde, or sodium benzoate;

[0019] The colorant is Acid Red, Congo Red, or Basic Rose Essence.

[0020] Furthermore, the application of the aforementioned suspension seed coating agent in the prevention and control of potato cyst nematode disease and late blight.

[0021] As can be seen from the above technical solution, compared with the prior art, this invention discloses a suspension seed dressing agent and its application in the control of potato cyst nematode disease and late blight. Compared with simple fungicides such as metalaxyl and fluopyram, or fluopyram alone, the suspension seed dressing agent of this invention can effectively reduce the dosage and cost, while expanding the scope of disease control. Fluopyram is currently widely used as a highly effective nematicide. Since this agent is also a fungicide, mixing it with metalaxyl and fluopyram enhances the fungicidal effect and reduces the risk of drug resistance in potato late blight, achieving the effect of simultaneously controlling potato nematode disease and potato late blight. Moreover, by using the seed dressing agent to treat seed potatoes before planting, a single application can effectively reduce the damage caused by potato cyst nematodes and late blight, making it an ideal product for potato disease control. Detailed Implementation

[0022] The technical solutions in the embodiments of the present invention will be clearly and completely described below. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0023] Example 1

[0024] Weigh the following raw materials according to the mass ratio: fluopyram 5%, metalaxyl 3.5%, fludioxonil 2.5%, triphenylethylphenol polyoxyethylene ether 5%, sodium dodecyl sulfate 2%, polyvinylpyrrolidone 3%, sodium carboxymethyl cellulose 0.15%, glycerol 2%, silicone defoamer 1%, potassium sorbate 1.5%, Acid Red 1%, and water to make up to 100%.

[0025] Preparation method of suspension seed coating agent: According to the formula requirements, accurately weigh each component and add the above components and deionized water to a beaker in sequence. Start the high-speed shear mixer and continue processing for 10 to 20 minutes to ensure that all components are fully mixed. Then, use a sand mill for fine grinding until more than 95% of the particles have a particle size of 1 to 5 micrometers and the average particle size is kept within 3 micrometers. The final red suspension is the suspension seed coating agent.

[0026] Example 2

[0027] Weigh the following raw materials according to the mass ratio: fluopyram 10%, metalaxyl 3%, fludioxonil 2%, nonylphenol polyoxyethylene ether 3%, separating powder 1%, polyvinyl alcohol 2%, cellulose ether 0.1%, glycerol 5%, silicone defoamer 3%, potassium sorbate 1.5%, basic rose extract 2%, and water to 100%. Prepare the suspension seed coating agent using the above raw materials according to the preparation method of Example 1.

[0028] Example 3

[0029] Weigh the following raw materials according to the mass ratio: fluopyram 15%, metalaxyl 2.5%, fludioxonil 1.5%, fatty alcohol polyoxyethylene ether 2%, sodium dodecylbenzenesulfonate 6%, hydroxypropyl cellulose 0.5%, magnesium aluminum silicate 3%, propylene glycol 1%, organosilicon defoamer 5%, potassium sorbate 1.5%, basic rose extract 2%, and water to 100%. Prepare a suspension seed coating agent using the above raw materials according to the preparation method of Example 1.

[0030] Example 4

[0031] Weigh the following raw materials according to the mass ratio: fluopyram 20%, metalaxyl 1.8%, fludioxonil 1.2%, calcium lignosulfonate 3%, sodium dodecyl sulfate 2%, sodium alginate 2%, diatomaceous earth 0.15%, ethylene glycol 2%, organosilicon defoamer 1%, sodium benzoate 1%, Acid Red 2%, and water to 100%. Prepare a suspension seed coating agent using the above raw materials according to the preparation method of Example 1.

[0032] Example 5

[0033] Weigh the following raw materials according to the mass ratio: fluopyram 40%, metalaxyl 1.8%, fludioxonil 1.2%, octylphenol polyoxyethylene ether 4%, sodium dodecyl sulfate 4%, gum arabic 2%, xanthan gum 0.15%, glycerol 2%, silicone defoamer 3%, industrial-grade formaldehyde 3%, Congo red 3%, and water to 100%. Prepare a suspension seed coating agent using the above raw materials according to the preparation method of Example 1.

[0034] Field efficacy trials

[0035] Test method:

[0036] Potato seed treatment was carried out before planting using the suspension seed coating agents prepared in Examples 1 to 5 respectively. The dosage of the suspension seed coating agent was 100 ml / 100 kg of seed potato. The suspension seed coating agent was diluted with water and sprayed onto the seed potato and stirred to make the solution evenly distributed on the surface of the seed potato. After coating, the seed potato was dried in a ventilated place. The suspension seed coating agent has good film-forming properties and uniform coating.

[0037] The treatment group was planted with coated seed potatoes, while the control group was planted with uncoated seed potatoes. The experiment was conducted in fields in the mountainous region of southwestern my country where potato cyst nematode disease and late blight have been reported. The potato variety used was Qingshu 9. Each treatment was replicated three times, with each plot measuring 45 m². 2 41.7% fluopyram suspension concentrate (Bayer AG) and metalaxyl-fludioxonil suspension seed coating agent (total active ingredient 62.5 g / L, of which metalaxyl is 37.5 g / L and fludioxonil is 25 g / L; Guangdong Zhenge Biotechnology Co., Ltd.) were used as control agents, with the suspension seed coating agent dosage being 100 ml / 100 kg seed potatoes.

[0038] 1) Seedling emergence rate survey:

[0039] The emergence rate was investigated at the budding stage after potato planting, and the calculation formula is as follows:

[0040] Emergence rate (%) = Number of seedlings / Total number of seeds sown x 100

[0041] 2) Prevention efficacy survey:

[0042] (1) Investigation on the efficacy of late epidemic disease prevention

[0043] During the peak period of potato late blight, leaf disease incidence was investigated, and the disease index and control efficacy were calculated. The grading standards and calculation formulas are as follows:

[0044] Late blight grading standards (GB / T 17980.34 National Standard of the People's Republic of China; Fungicide control of potato late blight in Field Efficacy Trial Criteria (I)):

[0045] Based on the area of ​​leaf lesions, the disease is classified into grades 0 to 9, as follows:

[0046] Grade 0: No lesions;

[0047] Grade 1: The area of ​​lesions accounts for less than 5% of the total leaf area;

[0048] Grade 3: The lesions cover 6-10% of the entire leaf area;

[0049] Level 5: The lesion area accounts for 11-20% of the total leaf area;

[0050] Level 7: The lesion area accounts for 21-50% of the total leaf area;

[0051] Level 9: The lesion area accounts for more than 50% of the total leaf area.

[0052]

[0053] (2) Survey on the control efficacy of potato cyst nematode disease

[0054] The number of sporangia per unit volume of soil in each test plot was counted before potato planting, and the number of sporangia per unit volume of soil and plant roots in each plot was counted after potato harvest. The sporangia reduction rate and control efficacy were calculated using the following formulas:

[0055]

[0056] The effects of suspension seed dressing on emergence rate and yield, as well as its control efficacy against potato cyst nematode disease and late blight, are shown in Table 1.

[0057] Table 1. Control efficacy of suspension seed coating agents against potato cyst nematode disease and late blight.

[0058]

[0059] As shown in Table 1, the seed potato emergence rate in each plot treated with the suspension seed coating agents of Examples 1-5 was not significantly different from that in the control plot, indicating that the suspension seed coating agents are safe for potato seedling emergence. The suspension seed coating agents of Examples 1-5 showed significantly better control efficacy against potato cyst nematode than the metalaxyl-fludioxonil suspension seed coating agent, and significantly better control efficacy against potato late blight than fluopyram suspension. Meanwhile, Examples 4 and 5 showed control efficacy against potato cyst nematode that was close to or even better than that of fluopyram alone, but Example 4 contained only half the amount of fluopyram, resulting in higher control efficiency. Furthermore, Examples 2, 4, and 5 showed similar control efficacy against potato late blight compared to the metalaxyl-fludioxonil suspension seed coating agent. A comprehensive comparison of yield-increasing effects revealed that Examples 4 and 5 showed the most significant yield-increasing effects. The above results indicate that the suspension seed coating agent of the present invention has a significant disease prevention and yield-increasing effect on potatoes. The agent combination (Example 4) with 20% of the total raw material mass of fungicide, 1.8% of the total raw material mass of metalaxyl-M, and 1.2% of the total raw material mass of fludioxonil is the most efficient.

[0060] The above description of the disclosed embodiments enables those skilled in the art to make or use the invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the general principles defined herein may be implemented in other embodiments without departing from the spirit or scope of the invention. Therefore, the invention is not to be limited to the embodiments shown herein, but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.

Claims

1. A flowable concentrate, characterized in that, By mass percent, the following components are contained: fluopyram 20%, mefenoxam 1.8%, fludioxonil 1.2%, calcium lignosulfonate 3%, sodium dodecyl sulfate 2%, sodium alginate 2%, diatomite 0.15%, ethylene glycol 2%, silicone defoaming agent 1%, sodium benzoate 1%, acid scarlet 2%, and water is supplemented to 100%.

2. A flowable concentrate characterized in that, By mass percent, the following components are contained: fluopyram 40%, mefenoxam 1.8%, fludioxonil 1.2%, octylphenol polyoxyethylene ether 4%, sodium dodecyl sulfate 4%, gum arabic 2%, xanthan gum 0.15%, glycerol 2%, silicone defoaming agent 3%, industrial-grade formaldehyde 3%, Congo red 3%, and water is supplemented to 100%.

3. The use of a suspension concentrate as claimed in claim 1 or 2 for controlling potato cyst nematode disease and late blight.

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