Oral liquid for treating cognitive impairment as well as preparation system and detection method thereof

Abstract

The invention discloses oral liquid for treating cognitive impairment and a preparation system and a detection method thereof.The oral liquid comprises a decocting device, a filtering device, a concentrating device, an alcohol precipitation device, a uniform stirring device, a storage device and a sterilization device, the storage device comprises a filling mechanism and a detection mechanism, the filling mechanism is used for filling the oral liquid, and the detection mechanism is used for detecting the oral liquid; the detection mechanism is used for detecting whether the filled bottles are sealed or not; the detection mechanism comprises a conveying assembly used for conveying the bottles, a vacuum assembly used for detecting whether the bottles are sealed or not in a vacuum mode and an identification assembly used for identifying whether the bottles leak liquid or not. The conveying assembly comprises a conveying frame, a rotating part arranged on the conveying frame and a conveying part used for conveying the conveying frame, and the rotating part is used for achieving simultaneous rotation of the multiple bottles; the conveying frame is provided with a plurality of inserting holes used for inserting bottles. The bottle cap sealing device has the effect of sealing a bottle and a bottle cap, and the effect that the product can obviously improve cognitive impairment is obviously obtained through experimental data analysis.

Description

Technical Field

[0001] The present application relates to the technical field of oral liquid production, and in particular to an oral liquid for treating cognitive dysfunction and a preparation system and detection method thereof. Background Art

[0002] Blood tonic generally has the problem of low iron absorption rate, and the effect is too slow; some products use simple chemical iron as the main ingredient, and the dosage is difficult to control. Excessive iron is deposited in brain tissue and bones, which damages health; many blood tonic products will irritate the gastrointestinal tract, replenishing blood but hurting the stomach; they have a bad taste and a strong iron smell, so people are reluctant to eat them. The fundamental treatment of anemia is to strengthen the spleen and replenish qi, restore the transportation and transformation functions of the spleen and stomach, and promote the absorption and utilization of iron. Tangible blood is born from invisible qi. Yangxue oral liquid is to replenish blood and qi, strengthen the spleen and stomach, and enrich the source of vitality; with jujube tonic blood, the yang will grow and the yin will grow, and the symptoms of anemia will be rapidly improved. Seabuckthorn has obvious antioxidant and hyperlipidemia reduction. For the treatment of anemia, iron therapy is currently used at home and abroad. However, the side effects of long-term and large-dose iron supplements, such as loss of appetite, nausea, vomiting, diarrhea, etc., are not easily accepted by patients. Yangxue oral liquid uses natural medicinal preparations to treat iron deficiency anemia. It has unique antioxidant and anti-ischemic effects, a pleasant taste, and can significantly improve the patient's sleep quality.

[0003] In the process of preparing the blood-nourishing oral liquid, the general process requires boiling, filtering, stirring, standing, filling and sterilization. During the filling process, the sealing of the bottle after filling needs to be tested. If there is a problem with the sealing between the bottle and the bottle cap, it will cause problems in the subsequent storage of the blood-nourishing oral liquid. Summary of the invention

[0004] In order to improve the sealing problem during the filling process, the present application provides an oral liquid for treating cognitive dysfunction and a preparation system and detection method thereof.

[0005] The present application provides a system for preparing an oral liquid for treating cognitive dysfunction using the following technical solution: An oral liquid for treating cognitive dysfunction and a preparation system thereof, comprising a decoction device, a filtering device, a concentrating device, an alcohol precipitation device, a stirring device, a storage device and a sterilizing device, wherein the storage device comprises a filling mechanism and a detection mechanism, wherein the filling mechanism is used to fill the oral liquid, and the detection mechanism is used to detect whether the bottle is sealed after filling; The detection mechanism includes a conveying component for conveying the bottle, a vacuum component for vacuum detecting whether the bottle is sealed, and an identification component for identifying whether the bottle is leaking; The conveying assembly includes a conveying frame, a rotating part arranged on the conveying frame, and a conveying part for conveying the conveying frame, wherein the rotating part is used to realize simultaneous rotation of multiple bottles; The conveying frame is provided with a plurality of insertion holes for inserting bottles.

[0006] Optionally, the vacuum assembly includes a vacuum chamber, an extraction part for evacuating the vacuum chamber, a clamping part for clamping the conveying rack, and a control part. The vacuum chamber is located above the conveying part, and the bottom of the vacuum chamber is open. The control part is used to control the opening / closing of the bottom of the vacuum chamber.

[0007] Optionally, the clamping part includes a clamping frame that is lifted and lowered in the vacuum chamber, two clamping jaws that move relative to each other, and a driving group that realizes the relative movement of the two clamping jaws, and the clamping jaws are used to clamp the two ends of the conveying frame.

[0008] Optionally, the identification component includes an identification rod movably disposed in the vacuum chamber, an abutment block disposed at the end of the identification rod, identification paper movably disposed on the abutment block, a winding portion for winding the identification paper, and a judgment portion for judging whether liquid is adhered to the identification paper, the movement direction of the identification rod is horizontally disposed and perpendicular to the movement direction of the conveying frame, the abutment block is provided with an abutment cavity, the abutment cavity is adapted to the bottle and the bottle cap, and the abutment cavity presses the connection position between the identification paper and the bottle and the bottle cap.

[0009] Optionally, the judgment part includes a judgment frame, a judgment block lifted and lowered on the judgment frame, a plurality of judgment papers placed on the judgment block, and a color sensor for identifying the color of the judgment paper, the judgment paper and the marking paper have different colors, and if liquid adheres to the marking paper, the judgment paper will adhere to the judgment paper, the judgment frame is fixed to the bottom of the abutment block, and the marking paper also passes through the judgment frame, the color sensor is opposite to the marking paper and is located behind the marking paper passing through the judgment block, and the color sensor is electrically connected to a buzzer.

[0010] Optionally, the judgment frame is further provided with a cylinder, the judgment block and the plurality of judgment papers move within the cylinder, the marking paper contacts and passes through the topmost judgment paper, and there is only one judgment paper located outside the top of the cylinder.

[0011] Optionally, the judgment frame is further elastically provided with a pressing plate above the cylinder, and the judgment frame is provided with a reciprocating portion for realizing the reciprocating motion of the pressing plate.

[0012] Optionally, the rotating part includes a rotating disk, two rotating pieces movably arranged on both sides of the rotating disk, two sliders, two springs and a power group for realizing simultaneous rotation of multiple rotating disks, the rotating piece is in contact with the outer wall of the bottle, the rotating disk is provided with two slide grooves, the slider is connected to the bottom of the rotating disk, the slider slides in the slide groove, the spring is located in the slide groove, one end of the spring is fixedly connected to the inner wall of the end of the slide groove, and the other end is fixedly connected to the corresponding end of the slider.

[0013] The present application also provides an oral liquid for treating cognitive dysfunction using the following technical solution: An oral liquid for treating cognitive dysfunction is prepared by adopting a preparation system for the oral liquid for treating cognitive dysfunction and comprises alum, sea buckthorn, hawthorn and jujube.

[0014] The present application also provides a method for detecting an oral liquid for treating cognitive dysfunction using the following technical solution: A method for detecting an oral liquid for treating cognitive dysfunction, comprising the following steps: Selection of drug solution; Animal grouping and dosing; Indicator observation and detection: Morris water maze experiment, through positioning navigation test and space exploration test to evaluate the changes in learning and memory of each group of mice; Congo red staining method to detect the SP of the mouse hippocampus. After the Morris water maze experiment, the mice were killed, brain tissue was extracted, and pathological sections were made. The SP formed by Aβ amyloid protein in the hippocampus of the mouse was stained red, and the cell nucleus was stained light blue. The number and size of SP formed by amyloid protein deposition in the brain tissue can be visually observed under the microscope; RT-qPCR experiments were used to detect the mRNA expression levels of PI3K, Akt, and TNF-α in the hippocampus tissue of mice; WB experiments were used to detect the levels of PI3K, p-PI3K, Akt, and TNF-α proteins in mouse hippocampal tissue.

[0015] In summary, the present application includes at least one of the following beneficial technical effects: 1. The conveying component can be conveyed by a belt, and the function of the vacuum component is to put the bottle to be tested into a vacuum environment. If there is a problem with the sealing of the bottle, the liquid in the bottle will flow out from the connection between the bottle and the bottle cap, and the marking component is used to mark the flowing liquid. In the prior art, it is photographed by a camera or an image. However, during the shooting process, if the flowing liquid is small, the dynamic effect of its flow is not easy to be found due to the viscosity of the liquid itself. Therefore, the present application uses the marking component to mark the flowing liquid; 2. When the clamping frame starts to descend and moves to the top of the conveying frame, the two clamping claws move toward the two ends of the conveying frame respectively until the conveying frame is clamped, and then the conveying frame is pulled into the vacuum chamber, and the control board starts to close the bottom opening of the vacuum chamber, and the vacuum chamber is evacuated through the extraction part until the vacuum chamber is in a vacuum state. At this time, if there is a sealing problem between the bottle and the bottle cap, the liquid in the bottle flows out from the connection between the bottle and the bottle cap. In the initial state, the label paper is located on the side of the abutment cavity facing the bottle. After the vacuum detection of the bottle is completed, the label rod and the abutment block can be moved toward the direction of the bottle. At the same time, part of the label paper can be released. The initial loose state of the label paper facilitates the abutment block to move the label paper to fully fit the connection position between the bottle and the bottle cap. When the bottle and the bottle cap are fully fitted, the power motor is controlled to start rotating. Under the action of the synchronous belt and multiple synchronous wheels, the effect of driving the rotating disk is achieved. If there is a liquid flow during the rotation of the bottle, the liquid will adhere to the label paper to complete the collection effect of the liquid; 3. In the initial state, only the judgment paper at the top is located outside the cylinder. As the marking paper is wound, if liquid adheres to the marking paper, and when the marking paper moves to the bottom of the abutment block, a part of the marking paper with liquid adhered to it faces the judgment paper. Due to the adhesion of the liquid in this application, the marking paper drives the judgment paper to move while being stretched and pulled, until the judgment paper moves to the position of the color sensor. The color sensor recognizes the color of the judgment paper and triggers the buzzer alarm, which proves that there is a sealing problem with the corresponding bottle itself. After the top judgment paper is adhered and moved away, it is necessary to control the judgment block to move in the cylinder to transport the top judgment paper to the outside of the cylinder. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 It is a schematic diagram of the overall structure of the first embodiment of the present application; Figure 2 yes Figure 1 A magnified schematic diagram of part A; Figure 3 is a schematic diagram of the identification components in Example 1 of the present application; Figure 4 is a schematic diagram of a judgment unit in Embodiment 1 of the present application; Figure 5 It is the Morris water maze trajectory diagram of each group of mice (Note: the upper figure is the trajectory of the positioning navigation experiment, and the lower figure is the trajectory of the space exploration experiment); Figure 6 is the Congo red staining image of the hippocampus of mice in each group (Note: Bar=200 μm, the black frame is SP); Figure 7 These are WB band images of the hippocampal tissues of mice in each group.

[0017] 1. Drawing reference numerals: 1. conveying frame; 2. rotating part; 3. transmission part; 4. socket; 5. vacuum chamber; 6. clamping frame; 7. clamping claw; 8. driving group; 9. identification rod; 10. abutment block; 11. identification paper; 12. winding roller; 13. abutment chamber; 14. judgment frame; 15. judgment block; 16. judgment paper; 17. color sensor; 18. cylinder; 19. extrusion plate; 20. cam; 21. reciprocating plate; 22. elastic member; 23. rotating disk; 24. rotating sheet; 25. slider; 26. spring; 27. slide groove; 28. power motor; 29. ​​synchronous belt; 30. synchronous wheel; 31. power rod. DETAILED DESCRIPTION

[0018] The following is combined with Figure 1-7 This application is described in further detail. Embodiment 1

[0019] The present application embodiment discloses a system for preparing an oral liquid for treating cognitive dysfunction. Figure 1-Figure 4 A preparation system for an oral liquid for treating cognitive dysfunction includes a decoction device, a filtering device, a concentrating device, an alcohol precipitation device, a stirring device, a storage device and a sterilizing device. The storage device includes a filling mechanism and a detection mechanism. The filling mechanism is used to fill the oral liquid. In this embodiment, the blood-nourishing oral liquid is prepared by using 4-8g of alum, 200-400g of seabuckthorn, 80-200g of hawthorn, 100-200g of jujube, 5-10g of vitamin C, 100-200g of sucrose, 2-8g of sodium benzoate, and appropriate amounts of auxiliary materials. Using the preparation system in the embodiment of the present application, the following preparation method is adopted: Take the raw materials, decoct and filter; concentrate the filtrate, stir evenly, let it stand and filter to adjust the pH value to 2.5-4.0, add water and stir evenly, let it stand, filter, fill and sterilize to obtain; in addition, the decoction, stirring and filling mechanisms in this application all adopt conventional means in the prior art, which will not be repeated here, but since the sealing of the filling can determine the quality of the product in subsequent transportation and storage, it is necessary to perform sealing inspection on the bottles after filling. Of course, in this embodiment, random inspection of the bottles after filling is sufficient.

[0020] The detection mechanism detects whether the bottle is sealed after filling. The detection mechanism includes a conveying component for conveying the bottle, a vacuum component for vacuum detecting whether the bottle is sealed, and an identification component for identifying whether the bottle is leaking; the conveying component can be conveyed by a belt, and the function of the vacuum component is to put the bottle to be detected into a vacuum environment. If there is a problem with the sealing of the bottle, the liquid in the bottle will flow out from the connection between the bottle and the bottle cap, and the identification component is used to identify the outflowing liquid. In the prior art, it is shot by video or image, but in the shooting process, if the outflowing liquid is less, due to the viscosity of the liquid itself, the dynamic effect of its flow is not easy to be found, so the present application uses an identification component to identify the outflowing liquid.

[0021] The conveying assembly includes a conveying frame 1, a rotating part 2 arranged on the conveying frame 1, and a conveying part 3 for conveying the conveying frame 1. The rotating part 2 is used to realize the simultaneous rotation of multiple bottles; the conveying part 3 can be conveyed by a belt conveyor in the prior art, and the conveying frame 1 is provided with multiple insertion holes 4 for inserting bottles. The inner diameter of the insertion hole 4 is larger than the maximum outer diameter of the bottle, which is convenient for the bottle to be inserted into the insertion hole 4. The rotating part 2 includes a rotating disk 23, two rotating pieces 24 movably arranged on both sides of the rotating disk 23, two sliders 25, two springs 26 and a power group for realizing the simultaneous rotation of multiple rotating disks 23. The rotating piece 24 is in contact with the outer wall of the bottle, and the rotating disk 23 is provided with two slide grooves 27. The slider 25 is connected to the bottom of the rotating disk 23, and the slider 25 slides in the slide groove 27. The spring 26 is located in the slide groove 27. One end of the spring 26 is fixedly connected to the inner wall of the end of the slide groove 27, and the other end is fixedly connected to the inner wall of the end of the slide groove 27. The corresponding ends of the slider 25 are fixedly connected. In the initial state, the distance between the two rotating pieces 24 is smaller than the maximum outer diameter of the bottle. Therefore, when the bottle is inserted into the insertion hole 4, the two rotating pieces 24 move in directions away from each other until the bottle is completely inserted between the two rotating pieces 24 and the bottom end of the bottle is located on the rotating disk 23. At this time, the spring 26 is in a compressed state. Under the elastic force of the spring 26, the two rotating pieces 24 squeeze the bottle. Under the friction between the rotating pieces 24 and the bottle, the bottle can rotate with the rotating disk 23.

[0022] The power group includes multiple power rods 31, multiple synchronous wheels 30, a synchronous belt 29 and a power motor 28. The power rod 31 is fixedly connected to the bottom of the rotating disk 23, and the bottom end of the power rod 31 is coaxially fixed with the synchronous wheel 30. The conveying frame 1 is provided with an inner cavity, and the synchronous wheel 30 is rotatably arranged in the inner cavity of the conveying frame 1. The synchronous belt 29 controls the multiple synchronous wheels 30 to rotate simultaneously. The power motor 28 is used to control the rotation of the synchronous wheels 30 at either end. The power rod 31 is rotatably connected to the conveying frame 1, and the power rod 31 penetrates into the inner cavity of the conveying frame 1. The number of synchronous wheels 30 is the same as that of the rotating disk 23. The synchronous belt 29 is sleeved on the outside of the multiple synchronous wheels 30, and the synchronous belt 29 drives the multiple synchronous wheels 30 to rotate together. The power motor 28 controls the synchronous wheels 30 at either end to rotate, and then under the transmission action of the synchronous belt 29, the multiple synchronous wheels 30 rotate, thereby achieving the effect of rotating multiple bottles together.

[0023] The vacuum assembly includes a vacuum chamber 5, an extraction part for evacuating the vacuum chamber 5, a clamping part for clamping the conveying rack 1, and a control part. The vacuum chamber 5 is located above the conveying part 3, and the bottom of the vacuum chamber 5 is an opening. The control part is used to control the opening / closing of the bottom of the vacuum chamber 5. The length of the vacuum chamber 5 is greater than the length of the conveying rack 1. The extraction part uses a vacuum pump device in the prior art. The control part includes a control panel and a control electric push rod. The control panel slides outside the bottom opening of the vacuum chamber 5, and the control electric push rod is used to control the movement of the control panel. The control panel is provided with a sealing ring, which is squeezed and deformed by the control panel and the outer wall of the vacuum chamber 5, but does not affect the normal movement of the control panel. When the control panel blocks the bottom opening of the vacuum chamber 5, the sealing effect of the sealing ring further ensures the sealing of the vacuum chamber 5.

[0024] The clamping part includes a clamping frame 6 which is lifted and lowered in the vacuum chamber 5, two clamping jaws 7 which move relative to each other, and a driving group 8 which realizes the relative movement of the two clamping jaws 7. The clamping jaws 7 are used to clamp the two ends of the conveying frame 1. The lifting and lowering of the clamping frame 6 is realized by an electric push rod. The clamping jaws 7 are adapted to the ends of the conveying frame 1. Of course, an electromagnet can also be provided on the clamping jaws 7 to further ensure the stability of the clamping jaws 7 on the conveying frame 1. The driving group 8 can adopt a double-headed threaded rod structure, and of course, it can also be realized by two electric push rods. In the initial state, the distance between the two clamping jaws 7 is greater than the length of the conveying frame 1. When the clamping frame 6 starts to descend and moves to the top of the conveying frame 1, the two clamping jaws 7 move toward the two ends of the conveying frame 1 respectively until the conveying frame 1 is clamped, and then the conveying frame 1 is pulled into the vacuum chamber 5, and the control board starts to close the bottom opening of the vacuum chamber 5.

[0025] The vacuum chamber 5 is evacuated by the extraction part until the vacuum chamber 5 is in a vacuum state. At this time, if there is a sealing problem between the bottle and the bottle cap, the liquid in the bottle flows out from the connection between the bottle and the bottle cap. In order to mark the flowing liquid, the marking component includes a marking rod 9 movably arranged in the vacuum chamber 5, an abutment block 10 arranged at the end of the marking rod 9, a marking paper 11 movably arranged on the abutment block 10, a winding part for winding the marking paper 11, and a judgment part for judging whether the marking paper 11 is adhered to the liquid. The movement direction is set horizontally and perpendicular to the movement direction of the conveyor frame 1. The abutment block 10 is provided with an abutment cavity 13. The abutment cavity 13 is adapted to the bottle and the bottle cap. The abutment cavity 13 presses the connection position of the label paper 11 with the bottle and the bottle cap. The abutment cavity 13 is adapted to the connection position of the bottle and the bottle cap, which is conducive to the label paper 11 to be completely fitted with the connection position of the bottle and the bottle cap. In the process of bottle rotation, it can ensure that most of the liquid can remain in the label paper 11 as much as possible. The label paper 11 can be made of a material that is easy to adhere to the liquid and will not be penetrated by the liquid.

[0026] In this embodiment, the movement of the identification rod 9 can be along the length direction of the conveying frame 1 and perpendicular to the movement direction of the conveying frame 1. The movement power in the two specific directions is realized by the electric push rod. The specific connection structure is no longer described in detail in this embodiment. Under the action of the above two movement directions, the abutment block 10 can be used to perform random inspection on any bottle. In this embodiment, it is determined that if any bottle has a problem, the entire conveying frame 1 itself will have a problem, so it is sufficient to take a bottle for sealing performance testing.

[0027] The winding part includes two winding motors and two winding rollers 12. The two winding rollers 12 are rotatably arranged on the upper and lower sides of the abutment block 10 respectively. The axial direction of the winding roller 12 is horizontally arranged and perpendicular to the movement direction of the label paper 11. The two winding motors control the movement of the two winding rollers 12 respectively. The two ends of the label paper 11 are fixed on the outer peripheral walls of the two winding rollers 12 respectively. In the initial state, the label paper 11 is located on the side of the abutment chamber 13 facing the bottle. After the vacuum detection of the bottle is completed, the label rod 9 and the abutment block 10 can be moved toward the direction of the bottle. At the same time, part of the label paper 11 can be released. The initial relaxed state of the label paper 11 facilitates the abutment block 10 to move the label paper 11 to fully fit the connection position between the bottle and the bottle cap. When the bottle and the bottle cap are fully fitted, the power motor 28 is controlled to start rotating. Under the action of the synchronous belt 29 and a plurality of synchronous wheels 30, the effect of driving the rotating disk 23 is achieved. If there is a liquid flow during the rotation of the bottle, the liquid will adhere to the label paper 11 to complete the collection effect of the liquid.

[0028] The judging part includes a judging frame 14, a judging block 15 which is lifted and lowered on the judging frame 14, a plurality of judging papers 16 placed on the judging block 15, and a color sensor 17 for identifying the color of the judging paper 16. The color of the judging paper 16 is different from that of the marking paper 11. If liquid adheres to the marking paper 11, it will adhere to the judging paper 16. The judging frame 14 is fixed at the bottom of the abutment block 10, and the marking paper 11 also passes through the judging frame 14. The color sensor 17 is opposite to the marking paper 11 and is located behind the marking paper 11 passing through the judging block 15. The color sensor 17 is electrically connected to a buzzer. In this embodiment, for example, the marking paper 11 is set to be green and the judging paper 16 is set to be red. The color sensor 17 can only identify the red judging paper 16. The color sensor 17 is connected to the buzzer through a PLC controller.

[0029] The judgment frame 14 is also provided with a cylinder 18, and the judgment block 15 and multiple judgment papers 16 are all movable in the cylinder 18. The identification paper 11 contacts and passes through the topmost judgment paper 16, and there is only one judgment paper 16 located outside the top of the cylinder 18. The judgment block 15 moves along the vertical direction on the judgment frame 14. The movement of the judgment block 15 is realized by an electric push rod. The judgment papers 16 are stacked on the judgment block 15. In this embodiment, the judgment papers 16 are selected to be adhered to by liquid and the friction between two adjacent judgment papers 16 is not enough to drive each other to move. That is, if there is liquid adhered to the identification paper 11, the identification paper 11 can adhere to the judgment paper 16, and when there is no liquid on the identification paper 11, the friction between the identification paper 11 and the judgment paper 16 is not enough to drive the judgment paper 16 to move, so as to avoid detection errors or waste of judgment paper 16.

[0030] In the initial state, only the judgment paper 16 at the top is located outside the cylinder 18. As the identification paper 11 is wound, if liquid adheres to the identification paper 11, and when the identification paper 11 moves to the bottom of the abutment block 10, a part of the identification paper 11 with liquid adhered to it is facing the judgment paper 16. Due to the adhesion of the liquid in this application, the identification paper 11 drives the judgment paper 16 to move while being stretched and pulled, until the judgment paper 16 moves to the position of the color sensor 17. The color sensor 17 recognizes the color of the judgment paper 16 and triggers the buzzer alarm, which proves that there is a sealing problem with the corresponding bottle itself. After the top judgment paper 16 is adhered and moved away, it is necessary to control the judgment block 15 to move in the cylinder 18 to transport the top judgment paper 16 to the outside of the cylinder 18.

[0031] The judging frame 14 is also elastically provided with an extrusion plate 19 above the cylinder 18. The judging frame 14 is provided with a reciprocating portion for realizing the reciprocating motion of the extrusion plate 19. The reciprocating portion includes a reciprocating motor, a cam 20, a reciprocating block and an elastic member 22. The extrusion plate 19 and the judging frame 14 are connected by the elastic member 22 to realize the elastic connection effect of the extrusion plate 19 and the judging frame 14. The rotating part of the cam 20 is rotatably connected to the judging frame 14. The output end of the reciprocating motor is coaxially fixed with the rotating part of the cam 20. The raised part of the cam 20 abuts against the reciprocating block. One end of the reciprocating block is fixedly connected to the side wall of the extrusion plate 19. The raised part of the cam 20 is connected to the rotating part of the cam 20. The bottom of the reciprocating block abuts; in order to avoid detection errors, if there is less liquid adhered to the marking paper 11, the marking paper 11 may not be able to absorb the judgment paper 16. Therefore, when the portion of the marking paper 11 with liquid adhered moves between the squeezing plate 19 and the judgment paper 16, the cam 20 is controlled to rotate, and the raised portion of the cam 20 is separated from the reciprocating block. Under the elastic force of the elastic member 22, the squeezing plate 19 begins to knock the back of the marking paper 11, that is, the side of the marking paper 11 with liquid adhered is knocked against the judgment paper 16, so that the marking paper 11 and the judgment paper 16 can be adhered and fastened, thereby greatly reducing the detection error.

[0032] The implementation principle of the system for preparing an oral liquid for treating cognitive dysfunction in an embodiment of the present application is as follows: when the bottle is inserted into the insertion hole 4, the two rotating plates 24 move in directions away from each other until the bottle is completely inserted between the two rotating plates 24, until the bottom end of the bottle is located on the rotating disk 23, and the spring 26 is in a compressed state. Under the elastic force of the spring 26, the two rotating plates 24 squeeze the bottle, and under the friction between the rotating plates 24 and the bottle, the bottle can rotate with the rotating disk 23.

[0033] When the clamping frame 6 starts to descend and moves to the top of the conveying frame 1, the two clamping claws 7 move toward the two ends of the conveying frame 1 respectively until the conveying frame 1 is clamped, and then the conveying frame 1 is pulled into the vacuum chamber 5, and the control board starts to close the bottom opening of the vacuum chamber 5, and the vacuum chamber 5 is evacuated through the extraction part until the vacuum chamber 5 is in a vacuum state. At this time, if there is a sealing problem between the bottle and the bottle cap, the liquid in the bottle flows out from the connection between the bottle and the bottle cap. In the initial state, the label paper 11 is located on the side of the abutment cavity 13 facing the bottle, and when the bottle vacuum detection is completed After completion, the identification rod 9 and the abutment block 10 can be moved toward the direction of the bottle. At the same time, part of the identification paper 11 can be released. The initial relaxed state of the identification paper 11 makes it easier for the abutment block 10 to move the identification paper 11 to fully fit the connection position between the bottle and the bottle cap. When the bottle and the bottle cap are fully fitted, the power motor 28 is controlled to start rotating. Under the action of the synchronous belt 29 and multiple synchronous wheels 30, the rotating disk 23 is driven. If there is liquid flow during the rotation of the bottle, the liquid will adhere to the identification paper 11, thereby completing the collection effect of the liquid.

[0034] In the initial state, only the judgment paper 16 at the top is located outside the cylinder 18. As the identification paper 11 is wound, if liquid adheres to the identification paper 11, and when the identification paper 11 moves to the bottom of the abutment block 10, a part of the identification paper 11 with liquid adhered to it is facing the judgment paper 16. Due to the adhesion of the liquid in this application, the identification paper 11 drives the judgment paper 16 to move while being stretched and pulled, until the judgment paper 16 moves to the position of the color sensor 17. The color sensor 17 recognizes the color of the judgment paper 16 and triggers the buzzer alarm, which proves that there is a sealing problem with the corresponding bottle itself. After the top judgment paper 16 is adhered and moved away, it is necessary to control the judgment block 15 to move in the cylinder 18 to transport the top judgment paper 16 to the outside of the cylinder 18. Embodiment 2

[0035] The embodiment of the present application discloses an oral liquid for treating cognitive dysfunction. The oral liquid for treating cognitive dysfunction is prepared by using the preparation system for the oral liquid for treating cognitive dysfunction in the embodiment, and comprises alum, sea buckthorn, hawthorn, jujube and the like. Embodiment 3

[0036] In this example, the mechanism of the "blood nourishing oral liquid" in this application in improving cognitive impairment in AD mice was studied, and it was found that the blood nourishing oral liquid in this application can significantly improve cognitive impairment in mice. In clinical and production practice, it was found that the existing technology needs to be improved in terms of efficacy, stability, and saving of raw materials in order to enhance the effect of the drug, prolong the shelf life, reduce the amount of drug used, and improve the adaptability of the drug.

[0037] See also Figure 5-Figure 7 The present application embodiment discloses a method for detecting an oral liquid for treating cognitive dysfunction, comprising the following steps: Selection of medicine liquid: choose blood nourishing oral liquid, composed of: alum, sea buckthorn, hawthorn, and jujube.

[0038] Animal grouping and drug administration: 40 APP / PS1 double transgenic mice were randomly divided into: 10 AD model group (Model group), 10 high-dose Yangxue oral liquid group (YX-G group), 10 medium-dose Yangxue oral liquid group (YX-Z group), and 10 medium-low Yangxue oral liquid group (YX-D group). 10 non-transgenic C57BL / 6 mice with the same background served as the blank group (Control group). The high-, medium-, and low-dose Yangxue oral liquid groups were administered with 2.6, 1.3, and 0.65 ml·kg -1 ·d -1 The model group and the normal group were gavaged with an equal amount of normal saline for 14 consecutive days.

[0039] Indicator observation and detection: Morris water maze experiment, through positioning navigation test and space exploration test to evaluate the changes in learning and memory of each group of mice; Congo red staining method to detect the SP of the mouse hippocampus. After the Morris water maze experiment, the mice were killed, brain tissue was extracted, and pathological sections were made. The SP formed by Aβ amyloid protein in the hippocampus of the mouse was stained red, and the cell nucleus was stained light blue. The number and size of SP formed by amyloid protein deposition in the brain tissue can be visually observed under the microscope; RT-qPCR experiments were used to detect the mRNA expression levels of PI3K, Akt, and TNF-α in the hippocampus tissue of mice; Mouse hippocampal tissue was isolated, total RNA was extracted, its concentration and purity were tested, reverse transcribed into cDNA, and a 20ul system (10 μl SYBR Green Supermix (2x), 0.6ul forward and reverse primers, 50ngcDNA, and the remaining volume was supplemented with ddH2O) was selected; the machine conditions were incubation at 95°C for 2 min, followed by 40 cycles, denaturation at 95°C for 5 s, and extension at temperature for 30 s according to different primers. GAPDH was used as an internal reference for standardization. Relative quantification method 2 was selected -ΔΔc The relative expression of each mRNA was calculated. The primer sequences were synthesized by Shanghai Jierui Company, see Table 1.

[0040] Table 1 Primer sequences

[0041] WB experiment was used to detect the protein content of PI3K, p-PI3K, Akt and TNF-α in mouse hippocampus tissue. The hippocampus tissue of each group of mice was taken, lysed and the total protein was extracted. The protein concentration was quantitatively determined by BCA method, and then the samples were loaded, electrophoresed, transferred to mold, blocked, primary antibody (β-actin, PI3K, p-PI3K, Akt, TNF-α, the dilution times were 1:10000, 1:1000, 1:1000, 1:1000, 1:1000, 1:1000), secondary antibody, and color development were performed. The grayscale of the bands was analyzed by ImageJ image software.

[0042] Compared with the Control group, the results of the Morris water maze test showed that there was no statistical difference in the swimming speed of mice in each group, indicating that there was no difference in the motor ability of mice in each group, and follow-up observation was possible; compared with the Control group, the total swimming distance and latency of the mice in the Model group increased, and the number of crossing the platform decreased, which was statistically significant ( P ﹤0.05); Compared with the Model group, the total swimming distance and latency of the mice in the YX-G and YX-Z groups were reduced, and the number of crossing the platform increased, which was statistically significant ( P ﹤0.05). Figure 5 , Table 2.

[0043] Table 2. Morris water maze test results of mice in each group

[0044] Note: Compared with the Control group: * P <0.05, ** P <0.01, △ P＞ 0.05; compared with the Model group: # P <0.05, ## P <0.01, □ P＞ 0.05.

[0045] Congo red staining results: Through microscopic comparison, it was found that many red or pink senile plaques (SP) formed by amyloid protein deposition were scattered in the hippocampus and cortex of mice in the Model group, while almost no SP was found in mice in the Control group. Several SPs were also scattered in the hippocampus and cortex of mice in each drug-treated group, but they were reduced to varying degrees compared with the Model group, and the SP reduction in the YX-G group was the most obvious. Figure 6 .

[0046] Effect of Yangxue oral liquid on the expression of mRNA related to PI3K / AKT pathway in mouse hippocampus tissue; Compared with the Control group, the expression levels of PI3K and AKT mRNA in the hippocampus of the Model group mice were decreased, and the expression level of TNF-α mRNA was increased, with statistically significant differences ( P ﹤0.05). Compared with the Model group, the expression levels of PI3K and AKT mRNA in the hippocampus tissue of mice in the YX-G group were increased, and the expression level of TNF-α gene was decreased, which was statistically significant ( P ﹤0.05). See Table 3.

[0047] Table 3. RT-qPCR experimental results of hippocampal tissues of mice in each group ( ±s) Group N PI3K AKT TNF-α Control 3 1.01±0.19 1.01±0.15 1.01±0.21 Model 3 <![CDATA[0.21±0.06 ** ]]> <![CDATA[0.19±0.05 ** ]]> <![CDATA[11.97±1.55 ** ]]> YX-D 3 <![CDATA[0.28±0.05 □ ]]> <![CDATA[0.29±0.11 □ ]]> <![CDATA[10.91±4.38 □ ]]> YX-Z 3 <![CDATA[0.47±0.19 □ ]]> <![CDATA[0.38±0.08 # ]]> <![CDATA[6.66±1.08 ## ]]> YX-G 3 <![CDATA[0.57±0.18 # ]]> <![CDATA[0.48±0.11 # ]]> <![CDATA[4.39±1.78 ## ]]> Note: Compared with the Control group: * P <0.05, ** P <0.01, △ P＞ 0.05; compared with the Model group: # P <0.05, ## P <0.01, □ P＞ 0.05.

[0048] Effect of Yangxue Oral Liquid on the Expression of PI3K / Akt Pathway Related Proteins in Mouse Hippocampus Compared with the Control group, there was no difference in the expression levels of PI3K and Akt proteins in the hippocampus tissue of the Model group, the expression level of p-PI3K protein was decreased, and the expression level of TNF-α protein was increased, which was statistically significant ( P ﹤0.05). Compared with the Model group, there was no difference in the expression levels of PI3K and Akt proteins in the hippocampus tissue of mice in the YX-G group, but the expression level of p-PI3K protein increased and the expression level of TNF-α protein decreased, which was statistically significant ( P ﹤0.05). ( Figure 7 , Table 4).

[0049] Table 4. WB experimental results of hippocampal tissue of mice in each group ( ±s) Group N PI3K / β-Actin p-PI3K / PI3K AKT / β-Actin TNF-α / β-Actin Control 3 1.28±0.23 1.14±0.18 1.14,0.35 0.32,0.05 Model 3 <![CDATA[1.24±0.15 △ ]]> <![CDATA[0.57±0.05 ** ]]> <![CDATA[0.98,0.07 △ ]]> <![CDATA[1.25,0.27 ** ]]> YX-D 3 <![CDATA[1.17±0.07 □ ]]> <![CDATA[0.66±0.11 □ ]]> <![CDATA[0.94,0.03 □ ]]> <![CDATA[1.08,0.13 □ ]]> YX-Z 3 <![CDATA[1.16±0.17 □ ]]> <![CDATA[0.83±0.16 # ]]> <![CDATA[1.07,0.16 □ ]]> <![CDATA[0.87,0.11 □ ]]> YX-G 3 <![CDATA[1.21±0.13 □ ]]> <![CDATA[1.03±0.12 ## ]]> <![CDATA[1.08,0.05 □ ]]> <![CDATA[0.62,0.13 ## ]]> Note: Compared with the Control group: * P <0.05, ** P<0.01, △ P＞ 0.05; compared with the Model group: # P <0.05, ## P <0.01, □ P＞ 0.05.

[0050] The above are all preferred embodiments of the present application, and the protection scope of the present application is not limited thereto. Therefore, any equivalent changes made according to the structure, shape, and principle of the present application should be included in the protection scope of the present application.

Claims

1. A preparation system for an oral liquid for treating cognitive dysfunction, comprising a decoction device, a filtering device, a stirring device, a concentration device, an alcohol precipitation device, a storage device and a sterilization device, characterized in that: The storage device comprises a filling mechanism and a detection mechanism, wherein the filling mechanism is used to fill the oral liquid, and the detection mechanism is used to detect whether the bottle is sealed after filling; The detection mechanism includes a conveying component for conveying the bottle, a vacuum component for vacuum detecting whether the bottle is sealed, and an identification component for identifying whether the bottle is leaking; The conveying assembly comprises a conveying frame (1), a rotating part (2) arranged on the conveying frame (1), and a conveying part (3) used for conveying the conveying frame (1), wherein the rotating part (2) is used for realizing simultaneous rotation of a plurality of bottles; The conveying frame (1) is provided with a plurality of insertion holes (4) for inserting bottles.

2. The preparation system of an oral liquid for treating cognitive dysfunction according to claim 1, characterized in that: The vacuum assembly comprises a vacuum chamber (5), an extraction portion for evacuating the vacuum chamber (5), a clamping portion for clamping the conveying frame (1), and a control portion. The vacuum chamber (5) is located above the conveying portion (3). The bottom of the vacuum chamber (5) is open. The control portion is used to control the opening / closing of the bottom of the vacuum chamber (5).

3. The preparation system of an oral liquid for treating cognitive dysfunction according to claim 2, characterized in that: The clamping portion comprises a clamping frame (6) which is lifted and lowered in the vacuum chamber (5), two clamping jaws (7) which move relative to each other, and a driving group (8) which realizes the relative movement of the two clamping jaws (7), wherein the clamping jaws (7) are used to clamp the two ends of the conveying frame (1).

4. The preparation system of an oral liquid for treating cognitive dysfunction according to claim 3, characterized in that: The marking assembly comprises a marking rod (9) movably arranged in the vacuum chamber (5), a contact block (10) arranged at the end of the marking rod (9), a marking paper (11) movably arranged on the contact block (10), a winding portion for winding the marking paper (11), and a judging portion for judging whether liquid adheres to the marking paper (11); the movement direction of the marking rod (9) is arranged horizontally and perpendicular to the movement direction of the conveying frame (1); the contact block (10) is provided with a contact cavity (13); the contact cavity (13) is adapted to the bottle and the bottle cap; the contact cavity (13) tightly contacts the connection position between the marking paper (11) and the bottle and the bottle cap.

5. The preparation system of an oral liquid for treating cognitive dysfunction according to claim 4, characterized in that: The judging part comprises a judging frame (14), a judging block (15) which is lifted and arranged on the judging frame (14), a plurality of judging papers (16) placed on the judging block (15), and a color sensor (17) for identifying the color of the judging paper (16); the judging paper (16) has a different color from the marking paper (11); if liquid adheres to the marking paper (11), the marking paper (11) adheres to the judging paper (16); the judging frame (14) is fixed to the bottom of the abutting block (10); the marking paper (11) also passes through the judging frame (14); the color sensor (17) is opposite to the marking paper (11) and is located behind the marking paper (11) passing through the judging block (15); and the color sensor (17) is electrically connected to a buzzer.

6. The system for preparing an oral liquid for treating cognitive dysfunction according to claim 5, characterized in that: The judgment frame (14) is also provided with a cylinder (18), the judgment block (15) and the plurality of judgment papers (16) are movable in the cylinder (18), the marking paper (11) contacts and passes through the topmost judgment paper (16), and there is only one judgment paper (16) located outside the top of the cylinder (18).

7. The system for preparing an oral liquid for treating cognitive dysfunction according to claim 6, characterized in that: The judging frame (14) is also elastically provided with a pressing plate (19) above the cylinder (18), and the judging frame (14) is provided with a reciprocating portion for realizing the reciprocating motion of the pressing plate (19).

8. The system for preparing an oral liquid for treating cognitive dysfunction according to claim 3, characterized in that: The rotating part (2) comprises a rotating disk (23), two rotating pieces (24) movably arranged on both sides of the rotating disk (23), two sliders (25), two springs (26) and a power group for realizing simultaneous rotation of a plurality of rotating disks (23); the rotating piece (24) is in contact with the outer wall of the bottle; the rotating disk (23) is provided with two slide grooves (27); the slider (25) is connected to the bottom of the rotating disk (23); the slider (25) slides in the slide groove (27); the spring (26) is located in the slide groove (27); one end of the spring (26) is fixedly connected to the inner wall of the end of the slide groove (27); and the other end is fixedly connected to the corresponding end of the slider (25).

9. An oral liquid for treating cognitive dysfunction, prepared by using the preparation system for the oral liquid for treating cognitive dysfunction according to any one of claims 1 to 9, characterized in that: Including alum, sea buckthorn, hawthorn and jujube.

10. A method for detecting an oral liquid for treating cognitive dysfunction, characterized in that: The method for testing the oral liquid for treating cognitive dysfunction described in claim 9 comprises the following steps: Selection of drug solution; Animal grouping and dosing; Indicator observation and detection: Morris water maze experiment, through positioning navigation test and space exploration test to evaluate the changes in learning and memory of each group of mice; Congo red staining method to detect the SP of the mouse hippocampus. After the Morris water maze experiment, the mice were killed, brain tissue was extracted, and pathological sections were made. The SP formed by Aβ amyloid protein in the hippocampus of the mouse was stained red, and the cell nucleus was stained light blue. The number and size of SP formed by amyloid protein deposition in the brain tissue can be visually observed under the microscope; RT-qPCR experiments were used to detect the mRNA expression levels of PI3K, Akt, and TNF-α in the hippocampus tissue of mice; WB experiments were used to detect the levels of PI3K, p-PI3K, Akt, and TNF-α proteins in mouse hippocampal tissue.

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