Manganese metal organic framework material with oxidase properties and its preparation method and application

Abstract

The present invention provides a manganese metal-organic framework material with oxidase properties, a preparation method, and an application thereof. The material is prepared by the following method: using 1,3,5-trimethic acid as an organic ligand, manganese acetate tetrahydrate as a manganese source, and a water-ethanol solution as a solvent, to obtain a manganese metal organic framework (MOF) precursor via a solvothermal synthesis technique; the manganese MOF precursor is post-synthetically modified using an oxidant, wherein the oxidant is a mixed solution of an aqueous alkali metal hydroxide solution and H2O2, and the ratio of the manganese MOF precursor to the alkali metal hydroxide is (400-600) mg:(0.5-2) mmol. By using specific types and amounts of manganese MOF precursors and oxidants, the resulting metal-organic framework nanomaterial has specific enzyme-like properties, can exert the function of uricase, and has optimal and high uricase activity under neutral pH conditions.

Description

Technical Field

[0001] The present invention relates to the technical field of manganese metal organic framework materials and enzyme simulation synthesis, in particular to a manganese metal organic framework material with oxidase properties and a preparation method and application thereof. Background Art

[0002] Natural uricase is a protein molecule that catalyzes the oxidation of uric acid. It possesses a precise amino acid sequence and a defined hierarchical structure. It exhibits high catalytic activity, substrate specificity, catalytic diversity, mild reaction conditions, and adjustable enzyme activity. However, natural uricase also has numerous drawbacks, including poor stability, difficulty in extraction, high cost, and susceptibility to inactivation by various physical and chemical factors.

[0003] Model enzymes are non-protein molecules synthesized using organic or other chemical methods. These molecules structurally and functionally mimic the structure, properties, mechanism of action, and chemical reactions of natural enzymes within organisms. Essentially, model enzymes are artificially synthesized catalysts with enzymatic properties. Compared to natural enzymes, these carefully designed non-protein catalysts exhibit unique advantages and features. These include: 1) their relatively simple and stable structure, which is immune to the physical and chemical degradation common to natural enzymes. This allows for a long catalyst life and a wider range of applications, particularly for efficient catalytic reactions under extreme conditions. 2) The design of model enzymes is highly flexible and controllable. Researchers can precisely tailor the catalytic properties of model enzymes to meet the needs of diverse catalytic reactions by adjusting synthesis conditions, molecular structure, and surface modification. 3) The synthesis of model enzymes is more straightforward and economical, making them suitable for large-scale industrial production and highly competitive in the catalyst market.

[0004] Metal-organic frameworks (MOFs) are highly porous materials formed by the combination of metal clusters and organic ligands. Their unique structural properties have led to widespread applications in gas storage, separation, and catalysis. Organic ligands in MOFs primarily include carboxylates, nitrogen heterocycles, and phosphonates. Carboxylates are organic compounds containing a carboxyl group (-COOH). They can form coordination bonds with metal ions via the oxygen atoms of the carboxyl groups, thereby participating in the structural construction of MOFs. These ligands typically possess one or more carboxyl groups and can be linear or aromatic. They can form monodentate or polydentate coordination patterns with metal ions. Transition metal clusters and carboxylates are readily assembled into MOFs with peroxidase properties, which are widely used in detection, medicine, and other fields. However, reports of MOFs with uricase properties are limited, and their activity is low. Therefore, the exploration and development of MOFs with high uricase activity is of great practical significance. Summary of the Invention

[0005] The technical problem to be solved by the present invention is to provide a manganese metal organic framework material with oxidase properties.

[0006] Another technical problem to be solved by the present invention is to provide a method for preparing the above-mentioned manganese metal organic framework material with oxidase properties.

[0007] Another technical problem to be solved by the present invention is to provide the application of the above-mentioned manganese metal organic framework material with oxidase properties.

[0008] The technical solution adopted in the present invention is:

[0009] A manganese metal-organic framework material with oxidase properties is prepared by the following method: a manganese metal organic framework (MOF) precursor is obtained by a solvothermal synthesis technique using 1,3,5-trimethylbenzene trimesic acid as an organic ligand, manganese acetate tetrahydrate as a manganese source, and a water-ethanol solution as a solvent; the manganese MOF precursor is post-synthetically modified with an oxidant, wherein the oxidant is a mixed solution of an aqueous alkali metal hydroxide solution and H2O2, and the amount ratio of the manganese MOF precursor to the alkali metal hydroxide is (400-600) mg:(0.5-2) mmol (converted to a concentration of 4-6 mg / mL:5-20 μmol / mL).

[0010] The obtained manganese metal-organic framework (MOFs) material with oxidase properties can be used as a highly active urate oxidase mimic material in the oxidation of uric acid.

[0011] Preferably, in the above-mentioned manganese metal organic framework material with oxidase properties, the oxidant is a mixed solution of sodium hydroxide aqueous solution and H2O2, and the dosage ratio of sodium hydroxide aqueous solution to H2O2 is (0.5-2) mmol: (10-30) μL.

[0012] Preferably, in the above-mentioned manganese metal organic framework material with oxidase properties, the ratio of the manganese MOFs precursor to sodium hydroxide is 500 mg:1.2 mmol (converted to a concentration of 5 mg / mL:12 μmol / mL).

[0013] Preferably, in the above-mentioned manganese metal organic framework material with oxidase properties, the ratio of the sodium hydroxide aqueous solution to H2O2 is 1.2 mmol:25 μL.

[0014] Preferably, the manganese metal organic framework material with oxidase properties has a morphology of two-dimensional nanosheets and contains Mn, C, and O elements.

[0015] The preparation method of the above-mentioned manganese metal organic framework material with oxidase properties comprises the following specific steps:

[0016] (1) Manganese acetate tetrahydrate, 1,3,5-trimethylbenzenecarboxylic acid and a water-ethanol solution are mixed and subjected to a solvothermal reaction to obtain a manganese MOFs precursor;

[0017] (2) The manganese MOFs precursor is mixed with an oxidant for post-synthesis modification, whereby the oxidant is a mixed solution of an aqueous alkali metal hydroxide solution and H2O2, and the amount ratio of the manganese MOFs precursor to the alkali metal hydroxide is (400-600) mg:(0.5-2) mmol (converted to a concentration of 4-6 mg / mL:5-20 μmol / mL).

[0018] Preferably, in the preparation method of the above-mentioned manganese metal-organic framework material with oxidase properties, the millimolar ratio of the manganese acetate tetrahydrate and 1,3,5-trimethylbenzene is (1-3):1, the dosage ratio of the manganese acetate tetrahydrate to the water-ethanol solution is (0.2-2) mmol: (30-60) mL, and the volume ratio of water to ethanol in the water-ethanol solution is (1-5):1.

[0019] Preferably, in the preparation method of the above-mentioned manganese metal organic framework material with oxidase properties, the millimolar ratio of the manganese acetate tetrahydrate and 1,3,5-trimethylbenzene is 2.7:1, and the amount ratio of the manganese acetate tetrahydrate to the water-ethanol solution is 0.4 mmol:55 mL.

[0020] Preferably, in the method for preparing the above-mentioned manganese metal organic framework material with oxidase properties, the volume ratio of water to ethanol in the water-ethanol solution is 4.5:1.

[0021] Preferably, in the method for preparing the above-mentioned manganese metal organic framework material with oxidase properties, the temperature of the solvent thermal reaction is 25 to 60° C., and the time is 2 to 8 hours.

[0022] Preferably, in the method for preparing the above-mentioned manganese metal organic framework material with oxidase properties, the temperature of the solvent thermal reaction is 60° C. and the time is 2 hours.

[0023] Preferably, in the above-mentioned method for preparing the manganese metal organic framework material with oxidase properties, the manganese MOFs precursor obtained in step (1) is sequentially subjected to solid-liquid separation, washing and drying.

[0024] Preferably, in the method for preparing the above-mentioned manganese metal organic framework material with oxidase properties, the solid-liquid separation method is centrifugation, and then the solid matter obtained by centrifugation is washed and dried.

[0025] Preferably, in the method for preparing the above-mentioned manganese metal organic framework material with oxidase properties, the washing is carried out using a water-ethanol solution, preferably, the washing is carried out using a water-ethanol solution (v / v=1 / 2).

[0026] Preferably, in the method for preparing the above-mentioned manganese metal organic framework material with oxidase properties, the washing is performed three times.

[0027] Preferably, in the preparation method of the above-mentioned manganese metal organic framework material with oxidase properties, the drying method is vacuum drying; the drying temperature is 40-90°C, preferably 50-60°C; and the drying time is 10-24 hours, preferably 12-15 hours.

[0028] Preferably, in the preparation method of the above-mentioned manganese metal organic framework material with oxidase properties, the oxidant is a mixed solution of sodium hydroxide aqueous solution and H2O2, and the dosage ratio of the sodium hydroxide aqueous solution to H2O2 is (0.5~2) mmol: (10~30) μL.

[0029] Preferably, in the method for preparing the above-mentioned manganese metal organic framework material with oxidase properties, the ratio of the manganese MOFs precursor to the sodium hydroxide aqueous solution is 500 mg:1.2 mmol (converted to a concentration of 5 mg / mL:12 μmol / mL).

[0030] Preferably, in the method for preparing the above-mentioned manganese metal organic framework material with oxidase properties, the ratio of the sodium hydroxide aqueous solution to H2O2 is 1.2 mmol:25 μL.

[0031] Preferably, in the method for preparing the above-mentioned manganese metal organic framework material with oxidase properties, the temperature of the post-synthesis modification is 25-35° C., and the time is 1-10 minutes.

[0032] Preferably, in the method for preparing the above-mentioned manganese metal organic framework material with oxidase properties, after the post-synthesis modification in step (2), the system is sequentially subjected to solid-liquid separation and washing.

[0033] Preferably, in the method for preparing the above-mentioned manganese metal organic framework material with oxidase properties, the solid-liquid separation method is centrifugation, and the solid matter obtained by centrifugation is washed and dried.

[0034] Preferably, in the method for preparing the above-mentioned manganese metal organic framework material with oxidase properties, the washing is performed using ultrapure water.

[0035] Preferably, in the method for preparing the above-mentioned manganese metal organic framework material with oxidase properties, the washing times are 3-5 times.

[0036] Preferably, in the preparation method of the above-mentioned manganese metal organic framework material with oxidase properties, the drying method is vacuum drying; the drying temperature is 40-90°C, preferably 50-60°C; and the drying time is 10-24 hours, preferably 12-15 hours.

[0037] The above-mentioned manganese metal organic framework material with oxidase properties is used as an enzyme-like (mimicking enzyme) application.

[0038] Preferably, in the above application, the enzyme is urate oxidase.

[0039] Preferably, in the above application, the manganese metal organic framework material with oxidase properties is used for oxidative degradation of uric acid.

[0040] Preferably, in the above application, the conditions for the degradation of uric acid by the manganese metal organic framework material with oxidase properties are: under the conditions of 10 mg / dL uric acid solution per 2 mL, the amount of the material is 8 mg, and the reaction time is 15 minutes.

[0041] Preferably, in the above application, the pH value at which the manganese metal organic framework material with oxidase properties degrades uric acid is 7.0.

[0042] The beneficial effects of the present invention are:

[0043] The above-mentioned manganese metal-organic framework material with oxidase properties adopts specific types and amounts of manganese MOFs precursors and oxidants, so that the obtained metal-organic framework nanomaterial has specific enzyme-like properties. As a uricase simulation material, it has a two-dimensional nanosheet structure, can play the role of uricase, has optimal uricase oxidase activity and high activity under neutral pH conditions, does not produce hydrogen peroxide after oxidizing uric acid, can efficiently catalyze the oxidation of uric acid to produce allantoin, and realizes the oxidative degradation of uric acid; its preparation method is easy to operate, the process is simple, does not require extreme reaction conditions such as high temperature, high pressure, and calcination, is environmentally friendly, and provides an efficient, environmentally friendly and safe solution for the oxidative degradation of uric acid. BRIEF DESCRIPTION OF THE DRAWINGS

[0044] Figure 1 The morphology and elemental composition of the manganese metal-organic framework material with urate oxidase properties prepared in Example 1 of the present invention, wherein (a) is a SEM image and (b) is an EDS spectrum image.

[0045] Figure 2 This is a Fourier transform infrared spectroscopy (FT-IR) result diagram of the manganese metal organic framework material with urate oxidase properties prepared in Example 1 of the present invention;

[0046] Figure 3 This is a graph comparing the enzyme activities of the manganese metal-organic framework material having uricase properties prepared in Example 1 of the present invention and the cerium metal-organic framework material also having uricase properties;

[0047] Figure 4 This is a graph showing the time-dependence of the enzyme activity of the manganese metal-organic framework material with urate oxidase properties prepared in Example 1 of the present invention;

[0048] Figure 5 This is a graph showing the concentration dependence of the enzyme activity of the manganese metal-organic framework material having urate oxidase properties prepared in Example 1 of the present invention;

[0049] Figure 6 This is a graph showing the HPLC test results of the uric acid oxidation reaction of the manganese metal organic framework material with urate oxidase properties prepared in Example 1 of the present invention to produce allantoin;

[0050] Figure 7 This is a graph showing the relative enzyme activity measurement results of the manganese metal-organic framework material with urate oxidase properties prepared in Example 1 of the present invention under different pH conditions. DETAILED DESCRIPTION

[0051] In order to enable those skilled in the art to better understand the technical solution of the present invention, the technical solution of the present invention is further described in detail below with reference to the accompanying drawings and specific implementation methods.

[0052] The raw materials used in the following examples were all commercially available.

[0053] Example 1

[0054] Preparation of a manganese metal-organic framework (MOFs) material with urate oxidase properties

[0055] 980.4 mg of Mn(CH3COO)2∙4H2O was dissolved in 400 mL of ultrapure water, and 315.2 mg of H3BTC was dissolved in 150 mL of a water-ethanol solution (v / v = 1 / 2). The Mn(CH3COO)2∙4H2O solution was added dropwise to the H3BTC solution with stirring at 60°C, resulting in the formation of a white precipitate. After a 2-hour reaction, the precipitate was collected from the reaction system by centrifugation, washed several times with a water-ethanol solution (v / v = 1 / 2), and dried in a vacuum oven at 50°C for 12 hours. This was recorded as the manganese MOF precursor.

[0056] 500 mg of the manganese metal-organic framework (MOF) precursor was dispersed in 100 mL of ultrapure water under ultrasonication to form a suspension. A 0.5 mL NaOH (0.475 mL, 2.5 M) / H₂O₂ (0.025 mL, 30 wt%) mixture was then added to the suspension and shaken for 2 minutes, causing the white material to turn black. The product was isolated by centrifugation and washed several times with ultrapure water until the pH of the supernatant was neutral. The product was then dried in a vacuum oven at 50°C for 12 hours to obtain a manganese metal-organic framework (MOF) material with oxidase properties.

[0057] The morphology and elemental composition of the manganese metal organic framework material with oxidase properties prepared in Example 1 are shown in FIG. Figure 1 , where (a) is the SEM image and (b) is the EDS spectrum. Figure 1 It can be seen that the prepared manganese metal organic framework material with oxidase properties contains Mn, C, and O elements and is a two-dimensional nanosheet.

[0058] The Fourier transform infrared spectrum (FT-IR) of the prepared manganese metal organic framework material with oxidase properties is shown in Figure 2 . Figure 2 The FT-IR spectra of H3BTC, Mn MOFs precursors and Mn MOFs with urate oxidase activity are shown. Figure 2 It can be seen that the manganese MOFs precursor and manganese MOFs materials have the highest peaks at 1612, 1558, 1433, and 1372 cm -1 The characteristic peak at 534 cm-1 is attributed to the carboxyl absorption peak of the ligand trimesic acid (H3TBS). The peaks of the manganese MOFs precursor and manganese MOFs material are at 534 cm-1 and 612 cm-1, respectively. -1The Mn-O stretching vibration characteristic peak appears. In addition, compared with trimesic acid (H3TBC), the manganese MOFs precursor and manganese MOFs material have a peak at 3400 cm -1 A broad and weak absorption peak appears at each location, corresponding to the vibration of the OH group in the structure, indicating that there are some bound water or hydroxyl groups in MnO2.

[0059] Example 2

[0060] Preparation of a manganese metal-organic framework (MOFs) material with urate oxidase properties

[0061] 367.6 mg of Mn(CH3COO)2∙4H2O was dissolved in 100 mL of ultrapure water, and 315.2 mg of H3BTC was dissolved in 300 mL of a water-ethanol solution (v / v = 1 / 2). The Mn(CH3COO)2∙4H2O solution was added dropwise to the H3BTC solution with stirring at 60°C, resulting in the formation of a white precipitate. After reacting for 2 hours, the precipitate was collected from the reaction system by centrifugation, washed several times with a water-ethanol solution (v / v = 1 / 2), and dried in a vacuum oven at 90°C for 12 hours. This was recorded as the manganese MOF precursor.

[0062] 400 mg of the manganese metal-organic framework (MOF) precursor was dispersed in 100 mL of ultrapure water under ultrasonication to form a suspension. A 0.5 mL NaOH (0.490 mL, 1 M) / H₂O₂ (0.01 mL, 30 wt%) mixture was then added to the suspension and shaken for 2 minutes, causing the white material to turn black. The product was isolated by centrifugation and washed several times with ultrapure water until the supernatant had a neutral pH. The product was then dried in a vacuum oven at 90°C for 12 hours to obtain a manganese metal-organic framework (MOF) material with uricase properties.

[0063] Example 3

[0064] Preparation of a manganese metal-organic framework (MOFs) material with urate oxidase properties

[0065] 1102.9 mg of Mn(CH3COO)2∙4H2O was dissolved in 450 mL of ultrapure water, and 315.2 mg of H3BTC was dissolved in 150 mL of a water-ethanol solution (v / v = 1 / 2). The Mn(CH3COO)2∙4H2O solution was added dropwise to the H3BTC solution with stirring at 60°C, resulting in the formation of a white precipitate. After reacting for 2 hours, the precipitate was collected from the reaction system by centrifugation, washed several times with a water-ethanol solution (v / v = 1 / 2), and dried in a vacuum oven at 40°C for 12 hours. This was recorded as the manganese MOF precursor.

[0066] 600 mg of the manganese metal-organic framework (MOF) precursor was dispersed in 100 mL of ultrapure water under ultrasonication to form a suspension. A 0.5 mL NaOH (0.47 mL, 4.25 M) / H₂O₂ (0.03 mL, 30 wt%) mixture was then added to the suspension and shaken for 2 minutes, causing the white material to turn black. The product was isolated by centrifugation and washed several times with ultrapure water until the supernatant reached a neutral pH. The product was then dried in a vacuum oven at 40°C for 12 hours to obtain a manganese metal-organic framework (MOF) material with uricase properties.

[0067] Example 4

[0068] Urate oxidase activity assay

[0069] 2 mL of 10 mg / dL uric acid solution (dissolved in potassium phosphate buffer, pH 6.0) and different masses (1 mg, 4 mg, 8 mg, 16 mg) of manganese MOFs or cerium MOFs with uricase activity (Donghao Liu, Ping Yang et al. Study on performance of mimic uricase and its application inenzyme-free analysis. Analytical and Bioanalytical Chemistry, 2021, 413(26):6571-6580) were added to the system and reacted at 37°C for 30 minutes. A blank group (without uric acid) was also set up, and the absorbance of the system was measured at 290 nm using a microplate reader. The absorbance of the system was plotted as the vertical axis and the mass of the uricase mimic was plotted as the horizontal axis.

[0070] The results are as follows Figure 3 As shown, under the same concentration conditions, the manganese MOFs material in Example 1 has higher activity in oxidative degradation of uric acid than the cerium MOFs material.

[0071] Example 5

[0072] Optimization of conditions for oxidative degradation of uric acid by manganese MOFs materials

[0073] This embodiment optimizes the conditions for degrading uric acid using manganese MOFs materials, including the amount of manganese MOFs materials used and the reaction time.

[0074] 2mL of 10mg / dL uric acid solution and 1-32mg of manganese MOFs material were added to the system and reacted at 37℃ for 30 minutes. A blank group (no uric acid) was set up and the absorbance of the system was measured at 290nm using a microplate reader. The maximum absorbance value of the system was set as 100% relative activity. The relative activity was used as the vertical axis, and the reaction time of the manganese MOFs material ( Figure 4 ), dosage( Figure 5 ) as the horizontal axis.

[0075] In summary, the optimal conditions for the degradation of uric acid by the manganese MOFs material finally selected are: 2 mL of 10 mg / dL uric acid solution, 8 mg of manganese MOFs material, and a reaction time of 15 minutes.

[0076] Example 6

[0077] HPLC detection of allantoin produced by uric acid oxidation in manganese MOFs

[0078] Chromatographic separation was performed using a TTOSOX C18 column (250 mm × 4.6 mm, 5 μm particle size) at 37°C with a flow rate of 0.3 mL / min. The mobile phase consisted of a 90:10 volume ratio of methanol (MeOH) and triethylamine phosphate (TEA-H3PO4) solution (8 mL of H3PO4 in 1000 mL of pure water, followed by adjustment of the pH to 2.0 with TEA). The injection volume was 20 μL, and analytes were detected using a diode array detector (DAD) at a wavelength of 210 nm.

[0079] Sample solution: 5 mg of manganese MOFs was mixed with 2 mL of uric acid (UA) solution (100 µmol / L, pH 6) and shaken at room temperature for 1 hour. The supernatant was then obtained by centrifugation (10,000 rpm, 3 minutes) and analyzed by HPLC-DAD.

[0080] Standard solutions: Shake uric acid (UA) solution (100 µmol / L, pH 6) and allantoin solution (100 µmol / L, pH 6) at room temperature for 1 hour. Centrifuge (10,000 rpm, 3 minutes) and analyze the supernatant by HPLC-DAD.

[0081] Three replicates of each solution were prepared to ensure accuracy.

[0082] The results are as follows Figure 6 As shown in Figure 3, after the catalytic reaction, a new chromatographic peak appeared in the uric acid (UA) solution at 5.8 minutes, which was consistent with the peak of allantoin in the standard solution.

[0083] Example 7

[0084] Determination of the optimal pH for oxidative degradation of uric acid by manganese MOFs

[0085] To a 2 mL system, a 10 mg / dL uric acid solution dissolved in potassium phosphate buffer at different pH values ​​(pH 5, 5.5, 6, 6.5, 7, 7.5, and 8) was added. Then, 8 mg of manganese MOFs was added and the reaction was allowed to proceed for 15 minutes. A blank control (no uric acid) was also prepared, and the absorbance of the system was measured at 290 nm using a microplate reader. The maximum absorbance was set as 100% relative activity, and the relative activity was plotted against pH on the horizontal axis.

[0086] The results are as follows Figure 7 It can be found that the optimal pH for the oxidation and degradation of uric acid by manganese MOFs material is 7.0.

[0087] The above-described embodiments are merely descriptions of preferred implementations of the present invention and are not intended to limit the scope of the present invention. Without departing from the spirit of the present invention, various modifications and improvements to the technical solutions of the present invention made by ordinary engineers and technicians in this field should fall within the scope of protection determined by the claims of the present invention.

Claims

1. A method for preparing a manganese metal organic framework material with oxidase properties, characterized by: A manganese metal hydride (MOF) precursor (MOF) was prepared by a solvothermal synthesis technique using 1,3,5-trimethylbenzenecarboxylic acid as an organic ligand, divalent manganese salt manganese acetate tetrahydrate Mn(CH3COO)2·4H2O as a manganese source, and a water-ethanol solution as a solvent. The manganese metal hydride (MOF) precursor was post-synthetically modified using an oxidant. The oxidant was a mixed solution of an aqueous alkali metal hydroxide solution and H2O2. The ratio of the manganese metal hydride precursor to the alkali metal hydroxide was (400-600) mg:(0.5-2) mmol.

2. The method for preparing a manganese metal organic framework material having oxidase properties according to claim 1, characterized in that: The oxidant is a mixed solution of sodium hydroxide aqueous solution and H2O2, and the dosage ratio of sodium hydroxide aqueous solution to H2O2 is (0.5-2) mmol: (10-30) μL.

3. The method for preparing a manganese metal organic framework material having oxidase properties according to claim 1, characterized in that: The ratio of the manganese MOFs precursor to sodium hydroxide is 500 mg:1.2 mmol.

4. The method for preparing a manganese metal organic framework material having oxidase properties according to claim 1, characterized in that: The millimolar ratio of the manganese acetate tetrahydrate to 1,3,5-trimethylbenzene is (1-3):1, the dosage ratio of the manganese acetate tetrahydrate to the water-ethanol solution is (0.2-2) mmol: (30-60) mL, and the volume ratio of water to ethanol in the water-ethanol solution is (1-5):

1.

5. The method for preparing a manganese metal organic framework material having oxidase properties according to claim 1, characterized in that: The temperature of the solvent thermal synthesis technology is 25 to 60° C. and the time is 2 to 8 hours.

6. A manganese metal organic framework material with oxidase properties, characterized by: It is prepared by the method according to any one of claims 1 to 5.

7. The manganese metal organic framework material with oxidase properties according to claim 6, characterized in that: Its morphology is two-dimensional nanosheets, containing Mn, C, and O elements.

8. Use of the manganese metal organic framework material with oxidase properties according to claim 6 or 7 as an enzyme-like substance.

9. The use according to claim 8, characterized in that: The enzyme is urate oxidase.

10. The use according to claim 9, characterized in that: The conditions for the enzyme-like enzyme to be used for oxidative degradation of uric acid are: 8 mg of the material per 2 mL of 10 mg / dL uric acid solution, 15 minutes of reaction time, and pH 7.0.

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