A method, apparatus and program product for identifying rare diseases of the gut

Abstract

The application relates to the field of intelligent medical treatment, in particular to a method, equipment and program product for identifying rare intestinal diseases. The method comprises the following steps: acquiring pathological data of a to-be-detected person; the pathological data comprises goblet cells, Paneth cells, neutrophilic granulocytes and plasma cells; the type of the rare intestinal disease of the to-be-detected person is determined based on the pathological data, the type of the rare intestinal disease comprises autoimmune enteropathy and common variant immunodeficiency; when any two or more of the following conditions are met, namely, the number of goblet cells is less than a preset threshold, the number of Paneth cells is less than a preset threshold, neutrophilic granulocyte infiltration occurs, and the number of plasma cells remains unchanged, the to-be-detected person is determined to have autoimmune enteropathy; and when the number of plasma cells is less than a preset threshold, the to-be-detected person is determined to have common variant immunodeficiency. The application can effectively distinguish the types of rare intestinal diseases and has good clinical value.

Description

TECHNICAL FIELD

[0001] The present application relates to the field of intelligent medical treatment, in particular to a method, device, program product and computer readable storage medium for identifying intestinal rare diseases. BACKGROUND

[0002] Autoimmune enteropathy (AIE) is extremely rare, with a global incidence of less than 1 / 100,000, and adult cases are even rarer, with only more than 200 cases reported. Common variable immunodeficiency (CVID) is a primary immunodeficiency disease, and patients with CVID gastrointestinal involvement are also rare. Both misdiagnosis or missed diagnosis will lead to delayed treatment of patients, serious complications, and affect the quality of life and even endanger life. In addition to both having diarrhea, malabsorption and other digestive symptoms, AIE and CVID can also have systemic manifestations such as fever and electrolyte imbalance, and can also involve other systems, such as repeated infections and autoimmune diseases in CVID, and AIE can also have autoimmune-related involvement of other organs, which is difficult to distinguish from clinical features. Although there are intestinal epithelial cell autoantibodies and other indicators in the diagnosis of AIE, the absence of antibodies cannot rule out the disease. CVID is mainly based on the decrease of serum immunoglobulin level, but other diseases may also have similar conditions. There is currently a lack of specific diagnostic indicators and diagnostic processes for AIE patients and CVID patients with gastrointestinal involvement. SUMMARY

[0003] To solve the above problems, the present application provides a method for identifying intestinal rare diseases, which specifically comprises:

[0004] Obtaining pathological data of a testee; the pathological data includes goblet cells, Paneth cells, neutrophils, and plasma cells;

[0005] Determining the intestinal rare disease type of the testee based on the pathological data, the intestinal rare disease type including autoimmune enteropathy and common variable immunodeficiency; when any two or more of the following conditions are met: the number of goblet cells is less than a preset threshold, the number of Paneth cells is less than a preset threshold, and neutrophil infiltration occurs, and the number of plasma cells remains unchanged, the autoimmune enteropathy is determined; when the number of plasma cells is less than a preset threshold, the common variable immunodeficiency is determined.

[0006] Optionally, the pathological data is replaced by the expression amount of immunoglobulin, and the intestinal rare disease type is determined based on the expression amount of immunoglobulin, the expression amount of immunoglobulin including one or more of the following: IgG, IgM, and IgA; when the expression amount of immunoglobulin is greater than a preset threshold, the autoimmune enteropathy is determined, otherwise, the common variable immunodeficiency is determined.

[0007] Optionally, the pathological data further comprises an immune protein expression amount, when the pathological data shows any two or more of the following: goblet cells less than a preset threshold, Paneth cells less than a preset threshold, immune protein expression amount greater than a preset threshold, and neutrophil infiltration, and the plasma cells are unchanged, the autoimmune enteropathy is determined; when the pathological data shows plasma cells less than a preset threshold and / or immune protein expression amount less than a preset threshold, the common variant immunodeficiency disease is determined.

[0008] The method further comprises type classification, and the classified subjects are obtained by classifying the subjects; and the intestinal rare disease type of the classified subjects is determined.

[0009] The type classification process is as follows:

[0010] Clinical data of the subjects are obtained;

[0011] Whether the subjects have intestinal rare diseases is determined based on the clinical data, and when chronic diarrhea occurs, the subjects are determined as suspected intestinal rare diseases; otherwise, the subjects are determined as needing further screening of infection, immunity and other disease causes;

[0012] Intestinal endoscopic data of the subjects determined as suspected intestinal rare diseases are obtained, and whether the subjects have small intestinal villous atrophy is determined based on the intestinal endoscopic data; when the intestinal endoscopic data show small intestinal villous atrophy, the subjects are determined as having small intestinal villous atrophy; otherwise, the subjects are determined as having other intestinal diseases.

[0013] Pathological data of the subjects determined as having small intestinal villous atrophy are obtained, and autoimmune enteropathy or common variant immunodeficiency disease is determined based on the pathological data.

[0014] Optionally, the pathological data is four indicators of the duodenum, and when any two or more of the following occur: goblet cells less than a preset threshold, Paneth cells less than a preset threshold, and neutrophil infiltration, and the plasma cells are unchanged, the subjects are determined as having autoimmune enteropathy; when the pathological data shows plasma cells less than a preset threshold, the subjects are determined as having common variant immunodeficiency disease.

[0015] The pathological data is replaced by IgG immunoglobulin expression amount, and autoimmune enteropathy or common variant immunodeficiency disease is determined based on the IgG immunoglobulin expression amount; when the IgG immunoglobulin expression amount is greater than a preset threshold, the subjects are determined as having autoimmune enteropathy; otherwise, the subjects are determined as having common variant immunodeficiency disease.

[0016] Optionally, the pathological data is replaced by IgM immunoglobulin expression amount, and autoimmune enteropathy or common variant immunodeficiency disease is determined based on the IgM immunoglobulin expression amount; when the IgM immunoglobulin expression amount is greater than a preset threshold, the subjects are determined as having autoimmune enteropathy; otherwise, the subjects are determined as having common variant immunodeficiency disease.

[0017] Optionally, the pathological data is replaced by IgA immunoglobulin expression, and the autoimmune enteropathy or the common variant immunodeficiency disease is determined by the IgA immunoglobulin expression; when the IgA immunoglobulin expression is greater than a preset threshold value, the autoimmune enteropathy is determined; otherwise, the common variant immunodeficiency disease is determined.

[0018] The determination of the autoimmune enteropathy or the common variant immunodeficiency disease further includes IgG immunoglobulin expression; when any two or more of the following conditions are met: the goblet cells are less than a preset threshold value, the Paneth cells are less than a preset threshold value, the IgG immunoglobulin expression is greater than a preset threshold value, and the neutrophil infiltration appears, and the plasma cells are unchanged, the autoimmune enteropathy is determined; when the pathological data shows that the plasma cells are less than a preset threshold value and / or the IgG immunoglobulin expression is less than a preset threshold value, the common variant immunodeficiency disease is determined.

[0019] Optionally, the determination of the autoimmune enteropathy or the common variant immunodeficiency disease further includes IgM immunoglobulin expression; when any two or more of the following conditions are met: the goblet cells are less than a preset threshold value, the Paneth cells are less than a preset threshold value, the IgM immunoglobulin expression is greater than a preset threshold value, and the neutrophil infiltration appears, and the plasma cells are unchanged, the autoimmune enteropathy is determined; when the pathological data shows that the plasma cells are less than a preset threshold value and / or the IgM immunoglobulin expression is less than a preset threshold value, the common variant immunodeficiency disease is determined.

[0020] Optionally, the determination of the autoimmune enteropathy or the common variant immunodeficiency disease further includes IgA immunoglobulin expression; when any two or more of the following conditions are met: the goblet cells are less than a preset threshold value, the Paneth cells are less than a preset threshold value, the IgA immunoglobulin expression is greater than a preset threshold value, and the neutrophil infiltration appears, and the plasma cells are unchanged, the autoimmune enteropathy is determined; when the pathological data shows that the plasma cells are less than a preset threshold value and / or the IgA immunoglobulin expression is less than a preset threshold value, the common variant immunodeficiency disease is determined.

[0021] Optionally, the determination of the autoimmune enteropathy or the common variant immunodeficiency disease further includes IgM immunoglobulin expression, IgG immunoglobulin expression; when any two or more of the following conditions are met: the goblet cells are less than a preset threshold value, the Paneth cells are less than a preset threshold value, the IgM immunoglobulin expression is greater than a preset threshold value, the IgG immunoglobulin expression is greater than a preset threshold value, and the neutrophil infiltration appears, and the plasma cells are unchanged, the autoimmune enteropathy is determined; when any one or more of the following conditions are met: the pathological data shows that the plasma cells are less than a preset threshold value, the IgM immunoglobulin expression is less than a preset threshold value, and the IgG immunoglobulin expression is less than a preset threshold value, the common variant immunodeficiency disease is determined.

[0022] Optionally, the judgment of the autoimmune enteropathy or the common variant immunological deficiency disease further comprises IgA immunoglobulin expression amount, IgG immunoglobulin expression amount; when any two or more of the following conditions occur: goblet cells are less than a preset threshold, Paneth cells are less than a preset threshold, IgA immunoglobulin expression amount is greater than a preset threshold, IgG immunoglobulin expression amount is greater than a preset threshold, and neutrophil infiltration occurs, and the plasma cells do not change, the autoimmune enteropathy is determined; when any one or more of the following conditions of the pathological data occurs: the plasma cells are less than a preset threshold, the IgA immunoglobulin expression amount is less than a preset threshold, and the IgG immunoglobulin expression amount is less than a preset threshold, the common variant immunological deficiency disease is determined.

[0023] Optionally, the judgment of the autoimmune enteropathy or the common variant immunological deficiency disease further comprises IgA immunoglobulin expression amount, IgM immunoglobulin expression amount; when any two or more of the following conditions occur: goblet cells are less than a preset threshold, Paneth cells are less than a preset threshold, IgM immunoglobulin expression amount is greater than a preset threshold, IgA immunoglobulin expression amount is greater than a preset threshold, and neutrophil infiltration occurs, and the plasma cells do not change, the autoimmune enteropathy is determined; when any one or more of the following conditions of the pathological data occurs: the plasma cells are less than a preset threshold, the IgM immunoglobulin expression amount is less than a preset threshold, and the IgA immunoglobulin expression amount is less than a preset threshold, the common variant immunological deficiency disease is determined.

[0024] Optionally, the judgment of the autoimmune enteropathy or the common variant immunological deficiency disease further comprises IgA immunoglobulin expression amount, IgM immunoglobulin expression amount, IgG immunoglobulin expression amount; when any two or more of the following conditions occur: goblet cells are less than a preset threshold, Paneth cells are less than a preset threshold, IgA immunoglobulin expression amount is greater than a preset threshold, IgM immunoglobulin expression amount is greater than a preset threshold, IgG immunoglobulin expression amount is greater than a preset threshold, and neutrophil infiltration occurs, and the plasma cells do not change, the autoimmune enteropathy is determined; when any one or more of the following conditions of the pathological data occurs: the plasma cells are less than a preset threshold, the IgA immunoglobulin expression amount is less than a preset threshold, the IgM immunoglobulin expression amount is less than a preset threshold, and the IgG immunoglobulin expression amount is less than a preset threshold, the common variant immunological deficiency disease is determined.

[0025] The pathological data is replaced by second intestinal endoscopic data, and the autoimmune enteropathy or the common variant immunological deficiency disease is determined through the second intestinal endoscopic data; when duodenal erosion and / or hyperemia occur, the autoimmune enteropathy is determined, otherwise, the common variant immunological deficiency disease is determined.

[0026] Optionally, the judging of the autoimmune enteropathy or the common variant immunological deficiency disease further comprises second intestinal endoscopic data, the autoimmune enteropathy or the common variant immunological deficiency disease is judged through the pathological data and the second intestinal endoscopic data, when any two or more of the following conditions occur: goblet cell is less than a preset threshold, Paneth cell is less than a preset threshold, neutrophil infiltration occurs, duodenal erosion and / or hyperemia, the autoimmune enteropathy is determined, and when the pathological data is less than a preset threshold and / or duodenal normal, the common variant immunological deficiency disease is determined.

[0027] The pathological data is replaced by second clinical data, the autoimmune enteropathy or the common variant immunological deficiency disease is judged through the second clinical data, when the duration of chronic diarrhea is less than a preset threshold, the autoimmune enteropathy is determined, otherwise, the common variant immunological deficiency disease is determined.

[0028] Optionally, the duration of chronic diarrhea is replaced by repeated respiratory tract infections, when the repeated respiratory tract infections occur, the common variant immunological deficiency disease is determined, otherwise, the autoimmune enteropathy is determined.

[0029] Optionally, the judging of the autoimmune enteropathy or the common variant immunological deficiency disease further comprises second clinical data, the autoimmune enteropathy or the common variant immunological deficiency disease is judged through the pathological data and the second clinical data, when any two or more of the following conditions occur: goblet cell is less than a preset threshold, Paneth cell is less than a preset threshold, neutrophil infiltration occurs, the duration of chronic diarrhea is less than a preset threshold, the autoimmune enteropathy is determined, and when the pathological data is less than a preset threshold and / or the duration of chronic diarrhea is greater than a preset threshold, the common variant immunological deficiency disease is determined.

[0030] The purpose of the present application is to provide a computer program product, which comprises a computer program or instructions thereon, the computer program or instructions are executed by a processor to realize the above-mentioned method for identifying rare diseases of the intestinal tract.

[0031] The purpose of the present application is to provide a computer device, which comprises a memory, a processor and a computer program or instructions stored on the memory, the computer program or instructions are executed by the processor to realize the above-mentioned method for identifying rare diseases of the intestinal tract.

[0032] The purpose of the present application is to provide a computer readable storage medium, which stores a computer program or instructions thereon, the computer program or instructions are executed by a processor to realize the above-mentioned method for identifying rare diseases of the intestinal tract.

[0033] The advantages of the present application are:

[0034] 1. Autoimmune enteropathy (AIE) and common variable immunodeficiency (CVID) patients with gastrointestinal involvement can all show chronic diarrhea, endoscopy and histopathology show small intestinal villus atrophy, and the differential diagnosis of the two is still difficult, the present application analyzes from three dimensions of clinical features, endoscopic features and pathological features, and proposes a judgment process for distinguishing AIE and CVID, for improving the efficiency and accuracy of clinical diagnosis.

[0035] 2. The identification process of AIE and CVID first divides the patients through the clinical features of chronic diarrhea and the intestinal endoscopic data of small intestinal villus atrophy, detects the duodenal four pathological indexes of the divided patients, when two or more of the three features of reduced goblet cells, reduced Paneth cells and neutrophil infiltration are present, and no plasma cell reduction is present, it is determined as AIE; when plasma cell reduction is present, it is determined as CVID; which can effectively distinguish the intestinal rare diseases of the divided patients, and is helpful for clinical diagnosis.

[0036] 3. For the divided patients, the present application also proposes the distinguishing indexes of the length of the course of chronic diarrhea, repeated respiratory tract infections, IgG, IgM and IgA immunoglobulin expression, duodenal erosion and / or hyperemia under endoscopy, to provide more distinguishing indexes to improve the accuracy and reliability of the distinction between AIE and CVID. BRIEF DESCRIPTION OF DRAWINGS

[0037] In order to more clearly illustrate the technical solutions in the embodiments of the present application, the drawings needed in the embodiment description will be briefly introduced. Obviously, the drawings in the following description are only some embodiments of the present application, and other drawings can also be obtained by those skilled in the art without creative labor.

[0038] Figure 1 The method flowchart for identifying intestinal rare diseases provided by the embodiments of the present application;

[0039] Figure 2 The system schematic diagram for identifying intestinal rare diseases provided by the embodiments of the present application;

[0040] Figure 3 The device schematic diagram for identifying intestinal rare diseases provided by the embodiments of the present application;

[0041] Figure 4 The diarrhea course characteristics of autoimmune enteropathy and common variable immunodeficiency patients with gastrointestinal involvement provided by the embodiments of the present application. A: Distribution characteristics of diarrhea course of AIE and CVID patients, the red line represents the median; B: Receiver operating characteristic (ROC) curve of diarrhea course (months).

[0042] Figure 5Immunological characteristics of patients with autoimmune enteropathy and common variant immunodeficiency with gastrointestinal involvement provided in embodiments of the present invention: levels of complement C3 (A) and immunoglobulins IgG (B), IgA (C), and IgM (D), with the red line representing the median. E: Receiver operating characteristic (ROC) curves for IgG, IgA, and IgM;

[0043] Figure 6 The following are the characteristic pathological features of patients with autoimmune enteropathy (AIE) and common variant immunodeficiency with gastrointestinal involvement (CVID) provided in this embodiment of the invention. A: Shortened intestinal villi and lack of plasma cells in CVID patients (40×, hematoxylin and eosin staining). B: Increased lymphocytes and normal goblet cells in CVID patients (200×, hematoxylin and eosin staining). C: Lack of plasma cells in the lamina propria of CVID patients (100×, CD138 immunohistochemical staining). D: Shortened villi in AIE patients (40×, hematoxylin and eosin staining). E: Neutrophil infiltration, increased lymphocytes, and loss of goblet cells and Paneth cells in AIE patients (200×, hematoxylin and eosin staining). F: Apoptotic bodies (arrow) in AIE patients (200×, hematoxylin and eosin staining).

[0044] Figure 7 The Kaplan-Meier relapse-free survival curves provided for embodiments of the present invention are as follows: A represents common variant immunodeficiency disease; B represents autoimmune enteropathy; and C represents a comparison between patients with autoimmune enteropathy and patients with common variant immunodeficiency disease. Detailed Implementation

[0045] To enable those skilled in the art to better understand the present invention, the technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings.

[0046] In some of the processes described in the specification, claims, and accompanying drawings of this invention, multiple operations appearing in a specific order are included. However, it should be clearly understood that these operations may not be executed in the order they appear herein, or may be executed in parallel. The operation numbers, such as S101, S102, etc., are merely used to distinguish different operations and do not represent any execution order. Furthermore, these processes may include more or fewer operations, and these operations may be executed sequentially or in parallel. It should be noted that the descriptions such as "first," "second," etc., in this document are used to distinguish different messages, devices, modules, etc., and do not represent a sequential order, nor do they limit "first" and "second" to different types.

[0047] Figure 1A schematic diagram of the method for identifying rare intestinal diseases provided in this embodiment of the invention specifically includes:

[0048] S101: Obtain pathological data of the subject; the pathological data includes goblet cells, Paneth cells, neutrophils, and plasma cells;

[0049] The pathological data consisted of four duodenal indicators, taking into account abnormalities in epithelial cell subsets and immune cell subsets.

[0050] In one specific embodiment, a retrospective study was conducted on patients diagnosed with acute arterial disease (AIE) and patients with CVID gastrointestinal involvement who were hospitalized and treated at Peking Union Medical College Hospital between June 2012 and May 2024. The latter group presented with gastrointestinal symptoms and evidence of gastrointestinal involvement confirmed by gastrointestinal endoscopy. Demographic characteristics, symptoms, medical history, auxiliary examinations, endoscopic and histopathological features, and long-term prognostic information were collected from both groups. Chi-square test, Fisher's exact test, and Student's test were used to evaluate the prognostic and long-term outcomes of the patients. t Differences between the two patient groups were compared using the Wilcoxon rank-sum test. The sensitivity and specificity for diagnosing AIE and CVID based on four pathological indicators were calculated. Cut-off values ​​and areas under the curve (AUC) for the diagnostic indicators (immunoglobulin levels and duration of diarrhea) were calculated based on ROC curves. Finally, Kaplan-Meier survival curves for disease recurrence were plotted for both groups, and the log-rank test was performed.

[0051] S102: Based on the pathological data, determine the type of rare intestinal disease of the subject. The rare intestinal disease types include autoimmune enteropathy and common variant immunodeficiency disease. When the pathological manifestations show any two or more of the following: goblet cells less than a preset threshold, Paneth cells less than a preset threshold, and neutrophil infiltration, and plasma cells remain unchanged, it is determined to be autoimmune enteropathy. When the pathological data shows that the plasma cells are less than a preset threshold, it is determined to be common variant immunodeficiency disease.

[0052] In one embodiment, the preset threshold is the value of various types of cells in a healthy state.

[0053] In one embodiment, the neutrophil infiltration is replaced by the expression level of immune proteins, and the type of rare intestinal disease is determined based on the expression level of immune proteins. The expression level of immune proteins includes one or more of the following: IgG, IgM, and IgA. When the expression level of immune proteins is greater than a preset threshold, it is determined to be an autoimmune enteropathy; otherwise, it is determined to be a common variant immunodeficiency disease.

[0054] In one embodiment, the method further includes type classification, obtaining classified test subjects by classifying them into types; and determining the intestinal rare disease type of the classified test subjects.

[0055] In one embodiment, the process of type division is:

[0056] Obtaining clinical data of the subject; determining whether there is a rare intestinal disease based on the clinical data; when chronic diarrhea occurs, determining that it is a "suspected rare intestinal disease"; otherwise, determining that it is "further screening for infection, immunity and other disease causes";

[0057] Obtaining intestinal endoscopic data of the subject determined as a suspected rare intestinal disease, and determining whether it is a small intestinal villous atrophy intestinal disease based on the intestinal endoscopic data; when the intestinal endoscopic data shows small intestinal villous atrophy, it is determined to be a small intestinal villous atrophy intestinal disease; otherwise, it is determined to be other intestinal diseases.

[0058] In one embodiment, the intestinal endoscopic data includes small intestinal villous atrophy or non-small intestinal villous atrophy.

[0059] The subject population with suspected rare intestinal disease types is further divided by detecting intestinal endoscopic data; when the intestinal endoscopic data shows small intestinal villous atrophy, it is divided into a small intestinal villous atrophy intestinal disease type; when the intestinal endoscopic data does not show small intestinal villous atrophy, it is divided into other intestinal disease types.

[0060] Obtaining pathological data of the subject determined as a small intestinal villous atrophy intestinal disease, and determining autoimmune enteropathy or common variant immunodeficiency disease based on the pathological data.

[0061] The pathological data is four indicators of duodenum; when any two or more of the following conditions occur: cup cells are less than a preset threshold, Paneth cells are less than a preset threshold, and neutrophil infiltration occurs, and plasma cells do not change, it is determined to be autoimmune enteropathy; when the pathological data shows that the plasma cells are less than a preset threshold, it is determined to be a common variant immunodeficiency disease.

[0062] AIE and CVID identification is performed on the subject of the small intestinal villous atrophy intestinal disease type; whether the pathological data meets the four indicators, i.e., when two or more of the following conditions occur: cup cells decrease, Paneth cells decrease, and neutrophil infiltration, and no plasma cells decrease, it is determined to be CVID.

[0063] In one embodiment, the pathological data is replaced by IgG immunoglobulin expression, and autoimmune enteropathy or common variant immunodeficiency disease is determined by IgG immunoglobulin expression; when the IgG immunoglobulin expression is greater than a preset threshold, it is determined to be autoimmune enteropathy; otherwise, it is determined to be a common variant immunodeficiency disease.

[0064] In one embodiment, the pathological data is replaced by the expression amount of IgM immunoglobulin, and the autoimmune enteropathy or the common variant immunodeficiency disease is determined by the expression amount of IgM immunoglobulin. When the expression amount of IgM immunoglobulin is greater than a preset threshold value, the autoimmune enteropathy is determined. Otherwise, the common variant immunodeficiency disease is determined.

[0065] In one embodiment, the pathological data is replaced by the expression amount of IgA immunoglobulin, and the autoimmune enteropathy or the common variant immunodeficiency disease is determined by the expression amount of IgA immunoglobulin. When the expression amount of IgA immunoglobulin is greater than a preset threshold value, the autoimmune enteropathy is determined. Otherwise, the common variant immunodeficiency disease is determined.

[0066] In one embodiment, the determination of the autoimmune enteropathy or the common variant immunodeficiency disease further includes the expression amount of IgG immunoglobulin. When any two or more of the following conditions are met: the goblet cells are less than a preset threshold value, the Paneth cells are less than a preset threshold value, the expression amount of IgG immunoglobulin is greater than a preset threshold value, and the neutrophil infiltration is present, and the plasma cells are unchanged, the autoimmune enteropathy is determined. When the pathological data shows that the plasma cells are less than a preset threshold value and / or the expression amount of IgG immunoglobulin is less than a preset threshold value, the common variant immunodeficiency disease is determined.

[0067] In one embodiment, the determination of the autoimmune enteropathy or the common variant immunodeficiency disease further includes the expression amount of IgM immunoglobulin. When any two or more of the following conditions are met: the goblet cells are less than a preset threshold value, the Paneth cells are less than a preset threshold value, the expression amount of IgM immunoglobulin is greater than a preset threshold value, and the neutrophil infiltration is present, and the plasma cells are unchanged, the autoimmune enteropathy is determined. When the pathological data shows that the plasma cells are less than a preset threshold value and / or the expression amount of IgM immunoglobulin is less than a preset threshold value, the common variant immunodeficiency disease is determined.

[0068] In one embodiment, the determination of the autoimmune enteropathy or the common variant immunodeficiency disease further includes the expression amount of IgA immunoglobulin. When any two or more of the following conditions are met: the goblet cells are less than a preset threshold value, the Paneth cells are less than a preset threshold value, the expression amount of IgA immunoglobulin is greater than a preset threshold value, and the neutrophil infiltration is present, and the plasma cells are unchanged, the autoimmune enteropathy is determined. When the pathological data shows that the plasma cells are less than a preset threshold value and / or the expression amount of IgA immunoglobulin is less than a preset threshold value, the common variant immunodeficiency disease is determined.

[0069] In one embodiment, the judgment of the autoimmune enteropathy or the common variant immunological deficiency disease further comprises IgM immunoglobulin expression amount, IgG immunoglobulin expression amount; when any two or more of the following conditions occur: goblet cell is less than a preset threshold, Paneth cell is less than a preset threshold, IgM immunoglobulin expression amount is greater than a preset threshold, IgG immunoglobulin expression amount is greater than a preset threshold, and neutrophil infiltration occurs, and the plasma cell does not change, the autoimmune enteropathy is determined; when any one or more of the following conditions of the pathological data occurs: the plasma cell is less than a preset threshold, the IgM immunoglobulin expression amount is less than a preset threshold, and the IgG immunoglobulin expression amount is less than a preset threshold, the common variant immunological deficiency disease is determined.

[0070] In one embodiment, the judgment of the autoimmune enteropathy or the common variant immunological deficiency disease further comprises IgA immunoglobulin expression amount, IgG immunoglobulin expression amount; when any two or more of the following conditions occur: goblet cell is less than a preset threshold, Paneth cell is less than a preset threshold, IgA immunoglobulin expression amount is greater than a preset threshold, IgG immunoglobulin expression amount is greater than a preset threshold, and neutrophil infiltration occurs, and the plasma cell does not change, the autoimmune enteropathy is determined; when any one or more of the following conditions of the pathological data occurs: the plasma cell is less than a preset threshold, the IgA immunoglobulin expression amount is less than a preset threshold, and the IgG immunoglobulin expression amount is less than a preset threshold, the common variant immunological deficiency disease is determined.

[0071] In one embodiment, the judgment of the autoimmune enteropathy or the common variant immunological deficiency disease further comprises IgA immunoglobulin expression amount, IgM immunoglobulin expression amount; when any two or more of the following conditions occur: goblet cell is less than a preset threshold, Paneth cell is less than a preset threshold, IgM immunoglobulin expression amount is greater than a preset threshold, IgA immunoglobulin expression amount is greater than a preset threshold, and neutrophil infiltration occurs, and the plasma cell does not change, the autoimmune enteropathy is determined; when any one or more of the following conditions of the pathological data occurs: the plasma cell is less than a preset threshold, the IgM immunoglobulin expression amount is less than a preset threshold, and the IgA immunoglobulin expression amount is less than a preset threshold, the common variant immunological deficiency disease is determined.

[0072] In one embodiment, the judgment of the autoimmune enteropathy or the common variant immunological deficiency disease further comprises IgA immunoglobulin expression, IgM immunoglobulin expression, IgG immunoglobulin expression; when any two or more of the following conditions occur: goblet cells are less than a preset threshold, Paneth cells are less than a preset threshold, IgA immunoglobulin expression is greater than a preset threshold, IgM immunoglobulin expression is greater than a preset threshold, IgG immunoglobulin expression is greater than a preset threshold, and neutrophil infiltration occurs, and plasma cells do not change, the autoimmune enteropathy is determined; when any one or more of the following conditions of the pathological data: plasma cells are less than a preset threshold, IgA immunoglobulin expression is less than a preset threshold, IgM immunoglobulin expression is less than a preset threshold, and IgG immunoglobulin expression is less than a preset threshold, the common variant immunological deficiency disease is determined.

[0073] In one embodiment, the pathological data is replaced by second intestinal endoscopic data, and the autoimmune enteropathy or the common variant immunological deficiency disease is determined by the second intestinal endoscopic data; when duodenal erosion and / or hyperemia occur, the autoimmune enteropathy is determined; otherwise, the common variant immunological deficiency disease is determined.

[0074] In one embodiment, the judgment of the autoimmune enteropathy or the common variant immunological deficiency disease further comprises second intestinal endoscopic data, and the autoimmune enteropathy or the common variant immunological deficiency disease is determined by the pathological data and the second intestinal endoscopic data; when any two or more of the following conditions occur: goblet cells are less than a preset threshold, Paneth cells are less than a preset threshold, neutrophil infiltration occurs, and duodenal erosion and / or hyperemia occur, the autoimmune enteropathy is determined; when the pathological data shows that plasma cells are less than a preset threshold and / or the duodenum is normal, the common variant immunological deficiency disease is determined.

[0075] In one embodiment, the pathological data is replaced by second clinical data, and the autoimmune enteropathy or the common variant immunological deficiency disease is determined by the second clinical data; when the duration of chronic diarrhea is less than a preset threshold, the autoimmune enteropathy is determined; otherwise, the common variant immunological deficiency disease is determined.

[0076] In one embodiment, the duration of chronic diarrhea is replaced by repeated respiratory tract infections; when repeated respiratory tract infections occur, the common variant immunological deficiency disease is determined; otherwise, the autoimmune enteropathy is determined.

[0077] In one embodiment, the judging of the autoimmune enteropathy or the common variable immunodeficiency disease further comprises second clinical data, the autoimmune enteropathy or the common variable immunodeficiency disease is judged by the pathological data and the second clinical data, when any two or more of the following conditions are met, the autoimmune enteropathy is determined: the goblet cell is less than a preset threshold, the Paneth cell is less than a preset threshold, the neutrophil infiltration appears, the chronic diarrhea duration is less than a preset threshold; when the pathological data shows that the plasma cell is less than a preset threshold and / or the chronic diarrhea duration is greater than a preset threshold, the common variable immunodeficiency disease is determined.

[0078] In one embodiment, for the method of not dividing the testee or dividing the testee, in identifying the AIE and the CVID, the discriminant index includes: four duodenal pathological indexes (goblet cell, Paneth cell, neutrophil, plasma cell), chronic diarrhea duration, repeated respiratory tract infection, immunoglobulin expression amount (IgG, IgA, IgM), duodenal erosion, hyperemia, and AIE and CVID are identified by one or more discriminant indexes.

[0079] In one embodiment, 26 AIE patients and 29 CVID patients with gastrointestinal involvement were enrolled. Compared with CVID patients with gastrointestinal involvement, AIE patients were older at diagnosis, had shorter duration of chronic diarrhea, more severe diarrhea, and more obvious weight loss, hypoalbuminemia and electrolyte disturbance. In addition, CVID patients with gastrointestinal involvement had a history of repeated respiratory tract infections, significantly decreased blood IgG, IgM and IgA levels, and significantly reduced B cell number in peripheral blood lymphocyte subsets, decreased CD4+T number and increased CD8+T number, and significantly inverted CD4+ / CD8. The cut-off value of blood IgG level for differentiating the two diseases was 5.265 g / L (the normal population reference range was 7-17), and the AUC was 0.989. Under endoscopy, most patients had small intestinal villus atrophy, and AIE patients were more likely to have duodenal erosion, hyperemia and other endoscopic active inflammatory manifestations. Further analysis of the histopathological features of 23 AIE and 24 CVID patients found that both had obvious shortening of small intestinal villi; compared with CVID, AIE patients had significant reduction or disappearance of goblet cells and Paneth cells, more obvious neutrophil infiltration, and more common apoptotic bodies; CVID patients had significant plasmacyte disappearance or reduction in the lamina propria and deep crypt of intestinal biopsy, and prominent lymphocyte infiltration in the deep crypt. In addition, most CVID patients had lymphoid follicles. In AIE and CVID patients with gastrointestinal involvement, the sensitivity of duodenal four pathological indicators for differentiating AIE was 96%, and the specificity was 100%. Long-term follow-up showed that patients of both diseases were prone to repeated episodes of diarrhea, and the median recurrence-free survival of CVID patients was slightly longer than that of AIE. AIE and CVID patients with gastrointestinal involvement both had small intestinal villus atrophy. Detailed history collection, targeted auxiliary examination, high-quality endoscopy and histopathological feature description provide a strong basis for the differential diagnosis of the two diseases.

[0080] In one embodiment, the present application statistically analyzes the clinical features, endoscopic features and pathological features of AIE and CVID, which are shown in Tables 1, 2 and 3, respectively.

[0081] Table 1 Summary and comparison of clinical features of autoimmune enteropathy and common variable immunodeficiency patients

[0082]

[0083] # Continuous variables conforming to normal distribution are expressed as mean ± standard deviation, continuous variables not conforming to normal distribution are expressed as median (interquartile range IQR: 25th percentile-75th percentile), and categorical variables are expressed as number (percentage).

[0084] Differences between two groups of continuous variables with normal distribution were assessed using Student's t-test, and differences between two groups of continuous variables without normal distribution were assessed using Wilcoxon rank-sum test. Categorical variables were analyzed using Chi-square test or Fisher's exact test.

[0085] Table 2 Summary and comparison of endoscopic features of autoimmune enteropathy and common variable immunodeficiency patients

[0086]

[0087] Categorical variables are expressed as numbers (percentages).

[0088] Categorical variables were analyzed using Chi-square test or Fisher's exact test.

[0089] Table 3 Summary and comparison of pathological features of autoimmune enteropathy and common variable immunodeficiency patients

[0090]

[0091]

[0092]

[0093]

[0094] Categorical variables are expressed as numbers (percentages).

[0095] Categorical variables were analyzed using Chi-square test or Fisher's exact test.

[0096] Significant intraepithelial lymphocyte (IEL) infiltration was defined as more than 40 lymphocytes per 100 epithelial cells.

[0097] In one embodiment, the present application statistically analyzed the immunoglobulin levels, different pathological criteria and single pathological features diagnosis, as shown in Table 4.

[0098] Table 4 Differences and optimal cut-off values of diagnostic performance of immunoglobulin levels, diarrhea duration for diagnosing autoimmune enteropathy or common variable immunodeficiency patients with gastrointestinal involvement based on receiver operating characteristic (ROC) curves

[0099]

[0100] Diarrhea duration of AIE and CVID is shown in Table 4. Immunoglobulin profiles of AIE and CVID were clearly divided, and immunological results are shown in Figure 4 Figure 5 ​, as shown in Table 4. The four pathological features characteristic of AIE and CVID are shown in Table 4. The effect of the four pathological features in discriminating the two rare intestinal diseases is shown in Table 5. The recurrence-free survival curves of AIE and CVID are shown in Table 5. Figure 6 Figure 7

[0101] Table 5 Sensitivity and specificity of four pathological criteria for discriminating AIE and CVID

[0102]

[0103] Sensitivity of diagnosing AIE = 22 / 23 = 96%;

[0104] Specificity of diagnosing AIE: 17 / 17 = 100%;

[0105] Sensitivity of diagnosing CVID = 17 / 17 = 100%;

[0106] Specificity of diagnosing CVID: 22 / 23 = 96%.

[0107] The embodiment of the present application also provides a computer program product or system, comprising a computer program which, when executed by a processor, implements the method steps of identifying the rare intestinal diseases described above.

[0108] Figure 2 The system for identifying the rare intestinal diseases provided by the embodiment of the present application specifically comprises:

[0109] The acquisition module acquires the pathological data of the subject to be tested; the pathological data comprises goblet cells, Paneth cells, neutrophils, and plasma cells.

[0110] The discrimination module discriminates the type of the rare intestinal disease of the subject to be tested based on the pathological data, wherein the type of the rare intestinal disease comprises autoimmune enteropathy and common variable immunodeficiency; when any two or more of the following conditions are met, i.e., the number of goblet cells is less than a preset threshold, the number of Paneth cells is less than a preset threshold, and neutrophil infiltration is observed, and the number of plasma cells remains unchanged, the autoimmune enteropathy is determined; when the number of plasma cells is less than a preset threshold, the common variable immunodeficiency is determined.

[0111] Figure 3 The device for identifying the rare intestinal diseases provided by the embodiment of the present application specifically comprises:

[0112] The memory is used to store program instructions, and the processor is used to call the program instructions, so as to implement any one of the methods for identifying the rare intestinal diseases described above.

[0113] ​​The embodiment of the present application further provides a computer readable storage medium, which stores a computer program, and the computer program is executed by a processor to implement the method for identifying rare intestinal diseases.

[0114] The verification result of the verification embodiment shows that assigning inherent weights to indications can improve the performance of the method compared with the default setting. It can be clearly understood by those skilled in the art that, for the convenience and brevity of description, the specific working processes of the system, device and unit described above can refer to the corresponding processes in the foregoing method embodiments, and will not be described here again. In the several embodiments provided by the present application, it should be understood that the disclosed system, device and method can be implemented in other ways. For example, the device embodiments described above are only schematic. For example, the division of the units is only a logical function division, and there can be another division manner in actual implementation. For example, a plurality of units or components can be combined or integrated into another system, or some features can be ignored or not executed. In addition, the coupling or direct coupling or communication connection between the units shown or discussed can be indirect coupling or communication connection through some interface, device or unit, and can be electrical, mechanical or other forms. The units described as separated components can or can not be physical separate units, and the units shown as separate components can or can not be physical units, that is, they can be located in one place, or can be distributed on a plurality of network units. Some or all of the units can be selected according to actual needs to achieve the purpose of the embodiment scheme. In addition, each functional unit in the various embodiments of the present application can be integrated in one processing unit, or each unit can be a physical unit, or two or more units can be integrated in one unit. The integrated unit can be in the form of hardware or in the form of software functional unit. Those skilled in the art can understand that all or part of the steps of the various methods in the above embodiments can be completed by a program instructing related hardware, and the program can be stored in a computer readable storage medium, and the storage medium can include read only memory (ROM), random access memory (RAM), magnetic disk or optical disk, etc.

[0115] Those skilled in the art can understand that all or part of the steps of the above-mentioned embodiment methods can be completed by a program instructing related hardware, and the program can be stored in a computer readable storage medium, and the storage medium mentioned above can be read only memory, magnetic disk or optical disk, etc.

[0116] The computer device provided by the present application is described in detail above, and for the general technical personnel in the field, the specific implementation manners and application ranges will be changed according to the idea of the embodiment of the present application, and the above description should not be understood as the limitation of the present application.

Claims

1. A system for identifying rare intestinal diseases, characterized in that, include: Acquisition module: Acquires pathological data of the subject; the pathological data includes goblet cells, Paneth cells, neutrophils, and plasma cells; The discrimination module determines the type of rare intestinal disease in the subject based on the pathological data. The rare intestinal disease types include autoimmune enteropathy and common variant immunodeficiency disease. When the pathological data shows any two or more of the following: goblet cells are less than a preset threshold, Paneth cells are less than a preset threshold, and neutrophil infiltration is present, and there is no decrease in plasma cell count, it is determined to be autoimmune enteropathy. When the pathological data shows that the plasma cell count is less than a preset threshold, it is determined to be common variant immunodeficiency disease. The preset threshold is the value of each type of cell in a healthy state.

2. The system for identifying rare intestinal diseases according to claim 1, characterized in that, The pathological data also includes the expression levels of immune proteins. When the pathological data shows that goblet cells are less than a preset threshold, Paneth cells are less than a preset threshold, or neutrophil infiltration occurs, and the expression level of immune proteins is greater than a preset threshold while plasma cells remain unchanged, it is determined to be an autoimmune enteropathy. When the pathological data shows that plasma cells are less than a preset threshold and the expression level of immune proteins is less than a preset threshold, it is determined to be a common variant immunodeficiency disease.

3. The system for identifying rare intestinal diseases according to any one of claims 1-2, characterized in that, The system also includes a type classification module, which classifies the test subjects into different types to obtain classified test subjects; and determines the type of rare intestinal disease of the classified test subjects.

4. The system for identifying rare intestinal diseases according to claim 3, characterized in that, The process of classifying the types is as follows: Obtain clinical data from test subjects; Based on the aforementioned clinical data, it is determined whether there is a rare intestinal disease. When chronic diarrhea occurs, it is determined to be a suspected rare intestinal disease; otherwise, it is determined to be a case for further screening of other disease causes such as infection and immune disorders. Acquire intestinal endoscopy data of subjects suspected of having rare intestinal diseases, and determine whether it is small intestinal villi atrophy enteropathies based on the intestinal endoscopy data. When small intestinal villi atrophy is observed in the intestinal endoscopy, it is determined to be small intestinal villi atrophy enteropathies; otherwise, it is determined to be other intestinal diseases. Obtain pathological data of individuals diagnosed with villous atrophic enteropathy, and determine whether the disease is autoimmune enteropathy or common variant immunodeficiency based on the pathological data.

5. The system for identifying rare intestinal diseases according to claim 4, characterized in that, The determination of autoimmune enteropathy or common variant immunodeficiency disease also includes the expression level of IgG immunoglobulin. When any two or more of the following are present: goblet cells are less than a preset threshold, Paneth cells are less than a preset threshold, and neutrophil infiltration is present, and the expression level of IgG immunoglobulin is greater than a preset threshold while the plasma cell count remains unchanged, it is determined to be autoimmune enteropathy. When the pathological data show that the plasma cell count is less than a preset threshold and the expression level of IgG immunoglobulin is less than a preset threshold, it is determined to be common variant immunodeficiency disease.

6. The system for identifying rare intestinal diseases according to claim 4, characterized in that, The determination of autoimmune enteropathy or common variant immunodeficiency disease also includes IgM immunoglobulin expression level. When any two or more of the following are present: goblet cells are below a preset threshold, Paneth cells are below a preset threshold, and neutrophil infiltration is present, and IgM immunoglobulin expression level is above a preset threshold while plasma cells remain unchanged, it is determined to be autoimmune enteropathy. When the pathological data shows that plasma cells are below a preset threshold and IgM immunoglobulin expression level is below a preset threshold, it is determined to be common variant immunodeficiency disease.

7. The system for identifying rare intestinal diseases according to claim 4, characterized in that, The determination of autoimmune enteropathy or common variant immunodeficiency disease also includes IgA immunoglobulin expression level. When any two or more of the following are present: goblet cells are below a preset threshold, Paneth cells are below a preset threshold, and neutrophil infiltration is present, and IgA immunoglobulin expression level is above a preset threshold while plasma cells remain unchanged, it is determined to be autoimmune enteropathy. When the pathological data shows that plasma cells are below a preset threshold and IgA immunoglobulin expression level is below a preset threshold, it is determined to be common variant immunodeficiency disease.

8. The system for identifying rare intestinal diseases according to claim 4, characterized in that, The determination of autoimmune enteropathy or common variant immunodeficiency disease also includes the expression levels of IgM immunoglobulin and IgG immunoglobulin. When any two or more of the following are present: goblet cells are below a preset threshold, Paneth cells are below a preset threshold, and neutrophil infiltration is present, and the expression levels of IgM immunoglobulin and IgG immunoglobulin are above a preset threshold, while plasma cells remain unchanged, it is determined to be autoimmune enteropathy. When the pathological data show that the plasma cells are below a preset threshold, the expression levels of IgM immunoglobulin are below a preset threshold, and the expression levels of IgG immunoglobulin are below a preset threshold, it is determined to be common variant immunodeficiency disease.

9. The system for identifying rare intestinal diseases according to claim 4, characterized in that, The determination of autoimmune enteropathy or common variant immunodeficiency disease also includes the expression levels of IgA immunoglobulin and IgG immunoglobulin. When any two or more of the following are present: goblet cells are below a preset threshold, Paneth cells are below a preset threshold, and neutrophil infiltration is present, and the expression levels of IgA immunoglobulin and IgG immunoglobulin are above a preset threshold, while plasma cells remain unchanged, it is determined to be autoimmune enteropathy. When the pathological data show that the plasma cells are below a preset threshold, the expression levels of IgA immunoglobulin are below a preset threshold, and the expression levels of IgG immunoglobulin are below a preset threshold, it is determined to be common variant immunodeficiency disease.

10. The system for identifying rare intestinal diseases according to claim 4, characterized in that, The determination of autoimmune enteropathy or common variant immunodeficiency disease also includes the expression levels of IgA immunoglobulin and IgM immunoglobulin. When any two or more of the following are present: goblet cells below a preset threshold, Paneth cells below a preset threshold, or neutrophil infiltration, and the expression levels of IgM immunoglobulin and IgA immunoglobulin are above a preset threshold, while plasma cells remain unchanged, it is determined to be autoimmune enteropathy. When the pathological data show that the plasma cells are below a preset threshold, the expression levels of IgM immunoglobulin are below a preset threshold, and the expression levels of IgA immunoglobulin are below a preset threshold, it is determined to be common variant immunodeficiency disease.

11. The system for identifying rare intestinal diseases according to claim 4, characterized in that, The determination of autoimmune enteropathy or common variant immunodeficiency disease also includes the expression levels of IgA immunoglobulin, IgM immunoglobulin, and IgG immunoglobulin. When any two or more of the following are present: goblet cells below a preset threshold, Paneth cells below a preset threshold, or neutrophil infiltration, and the expression levels of IgA immunoglobulin, IgM immunoglobulin, and IgG immunoglobulin are above a preset threshold, while plasma cells remain unchanged, it is determined to be autoimmune enteropathy. When the pathological data show that the expression levels of plasma cells, IgA immunoglobulin, IgM immunoglobulin, and IgG immunoglobulin are all below a preset threshold, it is determined to be common variant immunodeficiency disease.

12. The system for identifying rare intestinal diseases according to claim 1, characterized in that, The diagnosis of autoimmune enteropathy or common variant immunodeficiency disease also includes second enteroendoscopic data. Autoimmune enteropathy or common variant immunodeficiency disease is diagnosed based on the pathological data and second enteroendoscopic data. When goblet cells are less than a preset threshold, Paneth cells are less than a preset threshold, neutrophil infiltration is present, and duodenal erosion and / or congestion are present, it is diagnosed as autoimmune enteropathy. When the pathological data shows plasma cells less than a preset threshold and the duodenum is normal, it is diagnosed as common variant immunodeficiency disease.

13. The system for identifying rare intestinal diseases according to claim 1, characterized in that, The determination of autoimmune enteropathy or common variant immunodeficiency disease also includes second clinical data. Autoimmune enteropathy or common variant immunodeficiency disease is determined by the pathological data and the second clinical data. When goblet cells are less than a preset threshold, Paneth cells are less than a preset threshold, neutrophil infiltration is present, and the duration of chronic diarrhea is less than a preset threshold, it is determined to be autoimmune enteropathy. When the pathological data shows that plasma cells are less than a preset threshold and the duration of chronic diarrhea is greater than a preset threshold, it is determined to be common variant immunodeficiency disease.

14. A computer device comprising a memory, a processor, and a computer program or instructions stored in the memory, characterized in that, The computer program or instructions are executed by a processor to implement a method for identifying rare intestinal diseases, the method comprising: Obtain pathological data of the subject; the pathological data includes goblet cells, Paneth cells, neutrophils, and plasma cells; Based on the pathological data, the type of rare intestinal disease in the subject is determined. The rare intestinal disease types include autoimmune enteropathy and common variant immunodeficiency disease. When the pathological data shows any two or more of the following: goblet cells are less than a preset threshold, Paneth cells are less than a preset threshold, and neutrophil infiltration is present, and there is no decrease in plasma cell count, it is determined to be autoimmune enteropathy. When the pathological data shows that the plasma cell count is less than a preset threshold, it is determined to be common variant immunodeficiency disease. The preset threshold is the value of each type of cell in a healthy state.

15. The computer device according to claim 14, characterized in that, The pathological data also includes the expression levels of immune proteins. When the pathological data shows that goblet cells are less than a preset threshold, Paneth cells are less than a preset threshold, or neutrophil infiltration occurs, and the expression level of immune proteins is greater than a preset threshold while plasma cells remain unchanged, it is determined to be an autoimmune enteropathy. When the pathological data shows that plasma cells are less than a preset threshold and the expression level of immune proteins is less than a preset threshold, it is determined to be a common variant immunodeficiency disease.

16. The computer device according to any one of claims 14-15, characterized in that, The method also includes type classification, which involves classifying the test subjects into different types to obtain classified test subjects; and then determining the type of rare intestinal disease in the classified test subjects.

17. The computer device according to claim 16, characterized in that, The process of classifying the types is as follows: Obtain clinical data from test subjects; Based on the aforementioned clinical data, it is determined whether there is a rare intestinal disease. When chronic diarrhea occurs, it is determined to be a suspected rare intestinal disease; otherwise, it is determined to be a case for further screening of other disease causes such as infection and immune disorders. Acquire intestinal endoscopy data of subjects suspected of having rare intestinal diseases, and determine whether it is small intestinal villi atrophy enteropathies based on the intestinal endoscopy data. When small intestinal villi atrophy is observed in the intestinal endoscopy, it is determined to be small intestinal villi atrophy enteropathies; otherwise, it is determined to be other intestinal diseases. Obtain pathological data of individuals diagnosed with villous atrophic enteropathy, and determine whether the disease is autoimmune enteropathy or common variant immunodeficiency based on the pathological data.

18. The computer device according to claim 17, characterized in that, The determination of autoimmune enteropathy or common variant immunodeficiency disease also includes the expression level of IgG immunoglobulin. When any two or more of the following are present: goblet cells are less than a preset threshold, Paneth cells are less than a preset threshold, and neutrophil infiltration is present, and the expression level of IgG immunoglobulin is greater than a preset threshold while the plasma cell count remains unchanged, it is determined to be autoimmune enteropathy. When the pathological data show that the plasma cell count is less than a preset threshold and the expression level of IgG immunoglobulin is less than a preset threshold, it is determined to be common variant immunodeficiency disease.

19. The computer device according to claim 17, characterized in that, The determination of autoimmune enteropathy or common variant immunodeficiency disease also includes IgM immunoglobulin expression level. When any two or more of the following are present: goblet cells are below a preset threshold, Paneth cells are below a preset threshold, and neutrophil infiltration is present, and IgM immunoglobulin expression level is above a preset threshold while plasma cells remain unchanged, it is determined to be autoimmune enteropathy. When the pathological data shows that plasma cells are below a preset threshold and IgM immunoglobulin expression level is below a preset threshold, it is determined to be common variant immunodeficiency disease.

20. The computer device according to claim 17, characterized in that, The determination of autoimmune enteropathy or common variant immunodeficiency disease also includes IgA immunoglobulin expression level. When any two or more of the following are present: goblet cells are below a preset threshold, Paneth cells are below a preset threshold, and neutrophil infiltration is present, and IgA immunoglobulin expression level is above a preset threshold while plasma cells remain unchanged, it is determined to be autoimmune enteropathy. When the pathological data shows that plasma cells are below a preset threshold and IgA immunoglobulin expression level is below a preset threshold, it is determined to be common variant immunodeficiency disease.

21. The computer device according to claim 17, characterized in that, The determination of autoimmune enteropathy or common variant immunodeficiency disease also includes the expression levels of IgM immunoglobulin and IgG immunoglobulin. When any two or more of the following are present: goblet cells are below a preset threshold, Paneth cells are below a preset threshold, and neutrophil infiltration is present, and the expression levels of IgM immunoglobulin and IgG immunoglobulin are above a preset threshold, while plasma cells remain unchanged, it is determined to be autoimmune enteropathy. When the pathological data show that the plasma cells are below a preset threshold, the expression levels of IgM immunoglobulin are below a preset threshold, and the expression levels of IgG immunoglobulin are below a preset threshold, it is determined to be common variant immunodeficiency disease.

22. The computer device according to claim 17, characterized in that, The determination of autoimmune enteropathy or common variant immunodeficiency disease also includes the expression levels of IgA immunoglobulin and IgG immunoglobulin. When any two or more of the following are present: goblet cells are below a preset threshold, Paneth cells are below a preset threshold, and neutrophil infiltration is present, and the expression levels of IgA immunoglobulin and IgG immunoglobulin are above a preset threshold, while plasma cells remain unchanged, it is determined to be autoimmune enteropathy. When the pathological data show that the plasma cells are below a preset threshold, the expression levels of IgA immunoglobulin are below a preset threshold, and the expression levels of IgG immunoglobulin are below a preset threshold, it is determined to be common variant immunodeficiency disease.

23. The computer device according to claim 17, characterized in that, The determination of autoimmune enteropathy or common variant immunodeficiency disease also includes the expression levels of IgA immunoglobulin and IgM immunoglobulin. When any two or more of the following are present: goblet cells below a preset threshold, Paneth cells below a preset threshold, or neutrophil infiltration, and the expression levels of IgM immunoglobulin and IgA immunoglobulin are above a preset threshold, while plasma cells remain unchanged, it is determined to be autoimmune enteropathy. When the pathological data show that the plasma cells are below a preset threshold, the expression levels of IgM immunoglobulin are below a preset threshold, and the expression levels of IgA immunoglobulin are below a preset threshold, it is determined to be common variant immunodeficiency disease.

24. The computer device according to claim 17, characterized in that, The determination of autoimmune enteropathy or common variant immunodeficiency disease also includes the expression levels of IgA immunoglobulin, IgM immunoglobulin, and IgG immunoglobulin. When any two or more of the following are present: goblet cells below a preset threshold, Paneth cells below a preset threshold, or neutrophil infiltration, and the expression levels of IgA immunoglobulin, IgM immunoglobulin, and IgG immunoglobulin are above a preset threshold, while plasma cells remain unchanged, it is determined to be autoimmune enteropathy. When the pathological data show that the expression levels of plasma cells, IgA immunoglobulin, IgM immunoglobulin, and IgG immunoglobulin are all below a preset threshold, it is determined to be common variant immunodeficiency disease.

25. The computer device according to claim 14, characterized in that, The diagnosis of autoimmune enteropathy or common variant immunodeficiency disease also includes second enteroendoscopic data. Autoimmune enteropathy or common variant immunodeficiency disease is diagnosed based on the pathological data and second enteroendoscopic data. When goblet cells are less than a preset threshold, Paneth cells are less than a preset threshold, neutrophil infiltration is present, and duodenal erosion and / or congestion are present, it is diagnosed as autoimmune enteropathy. When the pathological data shows plasma cells less than a preset threshold and the duodenum is normal, it is diagnosed as common variant immunodeficiency disease.

26. The computer device according to claim 14, characterized in that, The determination of autoimmune enteropathy or common variant immunodeficiency disease also includes second clinical data. Autoimmune enteropathy or common variant immunodeficiency disease is determined by the pathological data and the second clinical data. When goblet cells are less than a preset threshold, Paneth cells are less than a preset threshold, neutrophil infiltration is present, and the duration of chronic diarrhea is less than a preset threshold, it is determined to be autoimmune enteropathy. When the pathological data shows that plasma cells are less than a preset threshold and the duration of chronic diarrhea is greater than a preset threshold, it is determined to be common variant immunodeficiency disease.

27. A computer-readable storage medium having a computer program or instructions stored thereon, characterized in that, The computer program or instructions are executed by a processor to implement a method for identifying rare intestinal diseases, the method comprising: Obtain pathological data of the subject; the pathological data includes goblet cells, Paneth cells, neutrophils, and plasma cells; The discrimination module determines the type of rare intestinal disease in the subject based on the pathological data. The rare intestinal disease types include autoimmune enteropathy and common variant immunodeficiency disease. When the pathological data shows any two or more of the following: goblet cells are less than a preset threshold, Paneth cells are less than a preset threshold, and neutrophil infiltration is present, and there is no decrease in plasma cell count, it is determined to be autoimmune enteropathy. When the pathological data shows that the plasma cell count is less than a preset threshold, it is determined to be common variant immunodeficiency disease. The preset threshold is the value of each type of cell in a healthy state.

28. The computer-readable storage medium according to claim 27, characterized in that, The pathological data also includes the expression levels of immune proteins. When the pathological data shows that goblet cells are less than a preset threshold, Paneth cells are less than a preset threshold, or neutrophil infiltration occurs, and the expression level of immune proteins is greater than a preset threshold while plasma cells remain unchanged, it is determined to be an autoimmune enteropathy. When the pathological data shows that plasma cells are less than a preset threshold and the expression level of immune proteins is less than a preset threshold, it is determined to be a common variant immunodeficiency disease.

29. The computer-readable storage medium according to any one of claims 27-28, characterized in that, The method also includes type classification, which involves classifying the test subjects into different types to obtain classified test subjects; and then determining the type of rare intestinal disease in the classified test subjects.

30. The computer-readable storage medium according to claim 29, characterized in that, The process of classifying the types is as follows: Obtain clinical data from test subjects; Based on the aforementioned clinical data, it is determined whether there is a rare intestinal disease. When chronic diarrhea occurs, it is determined to be a suspected rare intestinal disease; otherwise, it is determined to be a case for further screening of other disease causes such as infection and immune disorders. Acquire intestinal endoscopy data of subjects suspected of having rare intestinal diseases, and determine whether it is small intestinal villi atrophy enteropathies based on the intestinal endoscopy data. When small intestinal villi atrophy is observed in the intestinal endoscopy, it is determined to be small intestinal villi atrophy enteropathies; otherwise, it is determined to be other intestinal diseases. Obtain pathological data of individuals diagnosed with villous atrophic enteropathy, and determine whether the disease is autoimmune enteropathy or common variant immunodeficiency based on the pathological data.

31. The computer-readable storage medium according to claim 30, characterized in that, The determination of autoimmune enteropathy or common variant immunodeficiency disease also includes the expression level of IgG immunoglobulin. When any two or more of the following are present: goblet cells are less than a preset threshold, Paneth cells are less than a preset threshold, and neutrophil infiltration is present, and the expression level of IgG immunoglobulin is greater than a preset threshold while the plasma cell count remains unchanged, it is determined to be autoimmune enteropathy. When the pathological data show that the plasma cell count is less than a preset threshold and the expression level of IgG immunoglobulin is less than a preset threshold, it is determined to be common variant immunodeficiency disease.

32. The computer-readable storage medium according to claim 30, characterized in that, The determination of autoimmune enteropathy or common variant immunodeficiency disease also includes IgM immunoglobulin expression level. When any two or more of the following are present: goblet cells are below a preset threshold, Paneth cells are below a preset threshold, and neutrophil infiltration is present, and IgM immunoglobulin expression level is above a preset threshold while plasma cells remain unchanged, it is determined to be autoimmune enteropathy. When the pathological data shows that plasma cells are below a preset threshold and IgM immunoglobulin expression level is below a preset threshold, it is determined to be common variant immunodeficiency disease.

33. The computer-readable storage medium according to claim 30, characterized in that, The determination of autoimmune enteropathy or common variant immunodeficiency disease also includes IgA immunoglobulin expression level. When any two or more of the following are present: goblet cells are below a preset threshold, Paneth cells are below a preset threshold, and neutrophil infiltration is present, and IgA immunoglobulin expression level is above a preset threshold while plasma cells remain unchanged, it is determined to be autoimmune enteropathy. When the pathological data shows that plasma cells are below a preset threshold and IgA immunoglobulin expression level is below a preset threshold, it is determined to be common variant immunodeficiency disease.

34. The computer-readable storage medium according to claim 30, characterized in that, The determination of autoimmune enteropathy or common variant immunodeficiency disease also includes the expression levels of IgM immunoglobulin and IgG immunoglobulin. When any two or more of the following are present: goblet cells are below a preset threshold, Paneth cells are below a preset threshold, and neutrophil infiltration is present, and the expression levels of IgM immunoglobulin and IgG immunoglobulin are above a preset threshold, while plasma cells remain unchanged, it is determined to be autoimmune enteropathy. When the pathological data show that the plasma cells are below a preset threshold, the expression levels of IgM immunoglobulin are below a preset threshold, and the expression levels of IgG immunoglobulin are below a preset threshold, it is determined to be common variant immunodeficiency disease.

35. The computer-readable storage medium according to claim 30, characterized in that, The determination of autoimmune enteropathy or common variant immunodeficiency disease also includes the expression levels of IgA immunoglobulin and IgG immunoglobulin. When any two or more of the following are present: goblet cells are below a preset threshold, Paneth cells are below a preset threshold, and neutrophil infiltration is present, and the expression levels of IgA immunoglobulin and IgG immunoglobulin are above a preset threshold, while plasma cells remain unchanged, it is determined to be autoimmune enteropathy. When the pathological data show that the plasma cells are below a preset threshold, the expression levels of IgA immunoglobulin are below a preset threshold, and the expression levels of IgG immunoglobulin are below a preset threshold, it is determined to be common variant immunodeficiency disease.

36. The computer-readable storage medium according to claim 30, characterized in that, The determination of autoimmune enteropathy or common variant immunodeficiency disease also includes the expression levels of IgA immunoglobulin and IgM immunoglobulin. When any two or more of the following are present: goblet cells below a preset threshold, Paneth cells below a preset threshold, or neutrophil infiltration, and the expression levels of IgM immunoglobulin and IgA immunoglobulin are above a preset threshold, while plasma cells remain unchanged, it is determined to be autoimmune enteropathy. When the pathological data show that the plasma cells are below a preset threshold, the expression levels of IgM immunoglobulin are below a preset threshold, and the expression levels of IgA immunoglobulin are below a preset threshold, it is determined to be common variant immunodeficiency disease.

37. The computer-readable storage medium according to claim 30, characterized in that, The determination of autoimmune enteropathy or common variant immunodeficiency disease also includes the expression levels of IgA immunoglobulin, IgM immunoglobulin, and IgG immunoglobulin. When any two or more of the following are present: goblet cells below a preset threshold, Paneth cells below a preset threshold, or neutrophil infiltration, and the expression levels of IgA immunoglobulin, IgM immunoglobulin, and IgG immunoglobulin are above a preset threshold, while plasma cells remain unchanged, it is determined to be autoimmune enteropathy. When the pathological data show that the expression levels of plasma cells, IgA immunoglobulin, IgM immunoglobulin, and IgG immunoglobulin are all below a preset threshold, it is determined to be common variant immunodeficiency disease.

38. The computer-readable storage medium according to claim 27, characterized in that, The diagnosis of autoimmune enteropathy or common variant immunodeficiency disease also includes second enteroendoscopic data. Autoimmune enteropathy or common variant immunodeficiency disease is diagnosed based on the pathological data and second enteroendoscopic data. When goblet cells are less than a preset threshold, Paneth cells are less than a preset threshold, neutrophil infiltration is present, and duodenal erosion and / or congestion are present, it is diagnosed as autoimmune enteropathy. When the pathological data shows plasma cells less than a preset threshold and the duodenum is normal, it is diagnosed as common variant immunodeficiency disease.

39. The computer-readable storage medium according to claim 27, characterized in that, The determination of autoimmune enteropathy or common variant immunodeficiency disease also includes second clinical data. Autoimmune enteropathy or common variant immunodeficiency disease is determined by the pathological data and the second clinical data. When goblet cells are less than a preset threshold, Paneth cells are less than a preset threshold, neutrophil infiltration is present, and the duration of chronic diarrhea is less than a preset threshold, it is determined to be autoimmune enteropathy. When the pathological data shows that plasma cells are less than a preset threshold and the duration of chronic diarrhea is greater than a preset threshold, it is determined to be common variant immunodeficiency disease.

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