Traditional Chinese medicine compound for relieving contrast agent acute kidney injury

Abstract

The invention provides a traditional Chinese medicine compound for relieving contrast agent acute kidney injury. The traditional Chinese medicine compound is prepared from the following raw materials in parts by weight: 10-30 parts of astragalus membranaceus; 4-8 parts of prepared rhubarb; 8-12 parts of coptis chinensis; 1-20 parts of semen plantaginis; 10-20 parts of codonopsis pilosula; 10 to 20 parts of fructus psoraleae; 8-12 parts of rhizoma dioscoreae hypoglaucae; 4-8 parts of glossy privet fruit; and 2-4 parts of liquorice. The invention further provides a preparation method of the traditional Chinese medicine compound for relieving contrast agent acute kidney injury. The invention further provides a traditional Chinese medicine compound extracting agent for relieving contrast agent acute kidney injury. Astragalus mongholicus is used as a monarch drug for tonifying qi and nourishing yin; the rhizoma coptidis is used for clearing away heat and toxic materials, and the semen plantaginis and the rhizoma dioscoreae hypoglaucae are used for resolving dampness and inducing diuresis; radix codonopsis tonifies qi, fructus psoraleae and glossy privet fruit tonify kidney; and liquorice blending. The whole formula can prevent and relieve the contrast agent acute kidney injury, and has the effect of protecting the kidney function.

Description

Technical Field

[0001] The present invention belongs to the field of biomedicine, and relates to a traditional Chinese medicine complex, specifically a traditional Chinese medicine complex for relieving contrast-induced acute kidney injury. Background Art

[0002] Contrast-induced acute kidney injury (CI-AKI) is one of the common iatrogenic acute kidney injuries. With the wide application of contrast agents, the clinical incidence of contrast-induced kidney injury has increased significantly. Contrast-induced kidney injury can lead to rapid deterioration of renal function and even progress to renal failure. Alleviating contrast-induced acute kidney injury directly determines the recovery of the patient's renal function and whether contrast agents can be used for related treatments subsequently.

[0003] At present, the methods of western medicine for preventing and treating contrast-induced acute kidney injury are limited. Hypotonic and isotonic contrast agents are used, and at the same time, patients at risk of kidney injury who need to use contrast agents are given hydration and urine alkalinization treatment. However, once contrast-induced kidney injury occurs, western medicine still has no effective method to relieve the progression of CI-AKI.

[0004] Previously, we carried out a number of basic and clinical studies on the prevention and treatment of kidney diseases. In 2012, the relevant efficacy of astragalus injection on kidney injury in basic treatment was prospectively observed and statistically analyzed. The results showed that astragalus injection could significantly reduce serum creatinine, cystatin C, C-reactive protein, urinary albumin and urinary IgG in nephropathy patients; and no serious adverse reactions occurred.

[0005]

[0006] Table 1: Effects of astragalus injection on renal function (creatinine, urea nitrogen, uric acid and GFR) in nephropathy patients.

[0007]

[0008] Table 2: Effects of astragalus injection on serum cystatin C and C-reactive protein in patients with kidney injury.

[0009]

[0010] Table 3: Effects of astragalus injection on urinary albumin and urinary IgG in nephropathy patients. Summary of the Invention

[0011] In view of the above technical problems in the prior art, the present invention provides a traditional Chinese medicine complex for alleviating contrast-induced acute kidney injury, and this traditional Chinese medicine complex for alleviating contrast-induced acute kidney injury aims to solve the technical problem that the drugs in the prior art have poor effects in delaying and treating contrast-induced acute kidney injury.

[0012] The present invention provides a traditional Chinese medicine complex for alleviating contrast-induced acute kidney injury, which is prepared from the following raw materials in parts by weight:

[0013] Astragalus membranaceus 10 - 30;

[0014] Processed Rheum officinale 4 - 8;

[0015] Coptis chinensis 8 - 12;

[0016] Plantago asiatica 1 - 20;

[0017] Codonopsis pilosula 10 - 20;

[0018] Psoralea corylifolia 10 - 20;

[0019] Dioscorea hypoglauca 8 - 12;

[0020] Ligustrum lucidum 4 - 8;

[0021] Glycyrrhiza uralensis 2 - 4.

[0022] Furthermore, this traditional Chinese medicine complex for alleviating contrast-induced acute kidney injury is prepared from the following raw materials in parts by weight:

[0023] Astragalus membranaceus 20;

[0024] Processed Rheum officinale 6;

[0025] Coptis chinensis 10;

[0026] Plantago asiatica 15;

[0027] Codonopsis pilosula 15;

[0028] Psoralea corylifolia 15;

[0029] Dioscorea hypoglauca 10;

[0030] Ligustrum lucidum 6;

[0031] Glycyrrhiza uralensis 3.

[0032] Furthermore, the dosage form of the traditional Chinese medicine complex is tablets, capsules, granules, pills, powders, oral liquids, or aerosols.

[0033] Furthermore, the traditional Chinese medicine complex further includes a pharmaceutically acceptable carrier.

[0034] The present invention also provides a method for preparing the above-mentioned Chinese medicine composite for alleviating contrast-induced acute kidney injury, comprising the following steps:

[0035] 1) Weigh each Chinese medicine raw material according to weight;

[0036] 2) mixing dextrin and water, stirring and dispersing, and preparing a dextrin aqueous solution;

[0037] 3) a step of preparing a Chinese medicine extract, mixing and grinding astragalus, processed rhubarb, coptis root, plantain seed, codonopsis pilosula, psoralea corylifolia, radix ophiopogonis, fructus ligustris, and liquorice, adding 5-9 times the weight of deionized water, decocting 1-2 times, decocting for 1-2 hours each time, filtering, combining the filtrate, and concentrating under reduced pressure to obtain a water extract;

[0038] 4) mixing the dextrin aqueous solution and the water extract in a mass ratio of 1:1, stirring, and preparing a Chinese medicine extract-dextrin aqueous solution mixture; mixing the Chinese medicine extract-dextrin aqueous solution mixture with auxiliary materials, and vacuum drying to prepare granules.

[0039] The present invention also provides a Chinese medicine complex extractant for alleviating contrast agent-induced acute kidney injury. The extractant is prepared by extracting the above-mentioned Chinese medicine complex, wherein the extraction solvent of the extractant is water.

[0040] Furthermore, the extractant is a concentrated decoction or extract.

[0041] The present invention adopts astragalus as the main medicine for invigorating qi and nourishing yin; the processed rhubarb is used for dispelling turbidity and detoxification, coptis chinensis is used for clearing heat and detoxification, plantago seed and radix polygoni multiflori are used for removing dampness and promoting diuresis; codonopsis pilosula is used for invigorating qi, psoralea corylifolia and fructus ligustri lucidi are used for harmonizing, and the whole formula can prevent and alleviate contrast-induced acute kidney injury and has the effect of protecting kidney function.

[0042] The method of taking the drug of the present invention is: take one bag each time, twice a day, from three days before using the contrast agent to three days after using the contrast agent, or take it according to the doctor's advice.

[0043] Compared with the existing technology, the technical effect of the present invention is positive and obvious. Based on the previous astragalus drug treatment, the present invention has developed a Chinese medicine granule complex for preventing and alleviating contrast-induced acute kidney injury, which can reduce and prevent contrast-induced acute kidney injury, effectively prevent and delay the progression of CI-AKI, and has achieved good results in clinical practice. BRIEF DESCRIPTION OF THE DRAWINGS

[0044] Figure 1 The levels of serum creatinine in the treatment and control groups are shown. DETAILED DESCRIPTION

[0045] Demolition analysis:

[0046] Decoction formula 1 (6g of processed rhubarb, 10g of coptis, 15g of plantain seed, 15g of codonopsis pilosula, 15g of psoralea corylifolia, 10g of dioscorea hypoglauca, 6g of glossy privet fruit, 3g of licorice);

[0047] Decoction formula 2 (20g of astragalus membranaceus, 10g of coptis, 15g of plantain seed, 15g of codonopsis pilosula, 15g of psoralea corylifolia, 10g of dioscorea hypoglauca, 6g of glossy privet fruit, 3g of licorice);

[0048] Decoction formula 3 (20g of astragalus membranaceus, 6g of processed rhubarb, 15g of plantain seed, 15g of codonopsis pilosula, 15g of psoralea corylifolia, 10g of dioscorea hypoglauca, 6g of glossy privet fruit, 3g of licorice);

[0049] Decoction formula 4 (20g of astragalus membranaceus, 6g of processed rhubarb, 10g of coptis, 15g of codonopsis pilosula, 15g of psoralea corylifolia, 10g of dioscorea hypoglauca, 6g of glossy privet fruit, 3g of licorice);

[0050] Decoction formula 5 (20g of astragalus membranaceus, 6g of processed rhubarb, 10g of coptis, 15g of plantain seed, 15g of psoralea corylifolia, 10g of dioscorea hypoglauca, 6g of glossy privet fruit, 3g of licorice);

[0051] Decoction formula 6 (20g of astragalus membranaceus, 6g of processed rhubarb, 10g of coptis, 15g of plantain seed, 15g of codonopsis pilosula, 10g of dioscorea hypoglauca, 6g of glossy privet fruit, 3g of licorice);

[0052] Decoction formula 7 (20g of astragalus membranaceus, 6g of processed rhubarb, 10g of coptis, 15g of plantain seed, 15g of codonopsis pilosula, 15g of psoralea corylifolia, 6g of glossy privet fruit, 3g of licorice);

[0053] Decoction formula 8 (20g of astragalus membranaceus, 6g of processed rhubarb, 10g of coptis, 15g of plantain seed, 15g of codonopsis pilosula, 15g of psoralea corylifolia, 10g of dioscorea hypoglauca, 3g of licorice),

[0054] The present invention: 20g of astragalus membranaceus, 6g of processed rhubarb, 10g of coptis, 15g of plantain seed, 15g of codonopsis pilosula, 15g of psoralea corylifolia, 10g of dioscorea hypoglauca, 6g of glossy privet fruit, 3g of licorice.

[0055] Use astragalus membranaceus alone for treatment and give the contrast agent to the mice with acute kidney injury by gavage respectively for comparison of the treatment effects. Experimental research grouping: (6 mice in each group)

[0056] 1. Negative control group: Inject normal saline into the tail vein;

[0057] 2. Experimental group: Inject hypertonic contrast agent into the tail vein;

[0058] 3. Decoction formula 1 + experimental group: After injecting hypertonic contrast agent into the tail vein, continuously administer the drugs of decoction formula 1 by gavage for 7 days;

[0059] 4. Decoction formula 2 + experimental group: After injecting hypertonic contrast agent into the tail vein, continuously administer the drugs of decoction formula 2 by gavage for 7 days;

[0060] 5. Decoction Prescription 3 + Experimental Group: After tail vein injection of hypertonic contrast agent, continuously intragastrically administer the drug of Decoction Prescription 3 for 7 days;

[0061] 6. Decoction Prescription 4 + Experimental Group: After tail vein injection of hypertonic contrast agent, continuously intragastrically administer the drug of Decoction Prescription 3 for 7 days;

[0062] 7. Decoction Prescription 5 + Experimental Group: After tail vein injection of hypertonic contrast agent, continuously intragastrically administer the drug of Decoction Prescription 3 for 7 days;

[0063] 8. Decoction Prescription 6 + Experimental Group: After tail vein injection of hypertonic contrast agent, continuously intragastrically administer the drug of Decoction Prescription 3 for 7 days;

[0064] 9. Decoction Prescription 7 + Experimental Group: After tail vein injection of hypertonic contrast agent, continuously intragastrically administer the drug of Decoction Prescription 3 for 7 days;

[0065] 10. Decoction Prescription 8 + Experimental Group: After tail vein injection of hypertonic contrast agent, continuously intragastrically administer the drug of Decoction Prescription 3 for 7 days;

[0066] 11. Astragalus membranaceus Treatment Group: After tail vein injection of hypertonic contrast agent, continuously intragastrically administer the drug of Decoction Prescription 3 for 7 days;

[0067] 12. Treatment Group of the Present Invention: After tail vein injection of hypertonic contrast agent, continuously intragastrically administer the applied drug combination for 7 days;

[0068] Monitor the serum creatinine level respectively at the time of injection of the contrast agent, and on the 3rd, 5th, and 7th days.

[0069] The present invention also provides a preparation method of the above-mentioned traditional Chinese medicine complex for relieving acute kidney injury caused by contrast agent, including the following steps:

[0070] 1) Weigh each traditional Chinese medicine raw material according to the weight parts;

[0071] 2) Mix dextrin and water, stir and disperse to prepare a dextrin aqueous solution;

[0072] 3) A step of preparing a traditional Chinese medicine extract: Mix and pulverize Astragalus membranaceus, processed Rheum palmatum, Coptis chinensis, Plantago asiatica, Codonopsis pilosula, Psoralea corylifolia, Dioscorea hypoglauca, Ligustrum lucidum, and Glycyrrhiza uralensis, add deionized water with a weight 5 times that of the raw materials, decoct twice, each time for 2 hours, filter, combine the filtrates, and concentrate under reduced pressure to obtain an aqueous extract;

[0073] 4) Mix the dextrin aqueous solution and the aqueous extract according to a mass ratio of 1:1, stir to prepare a mixed solution of traditional Chinese medicine extract - dextrin aqueous solution; Mix the mixed solution of traditional Chinese medicine extract - dextrin aqueous solution and excipients, and vacuum dry to make granules.

[0074] The preparation method of the above-mentioned decoction prescription traditional Chinese medicine complex and the preparation method of the Astragalus membranaceus treatment group are implemented with reference to the above description.

[0075] Such as Figure 1It was found by monitoring serum creatinine levels that: the creatinine level in the treatment group of the present invention was significantly lower than that in the Astragalus membranaceus treatment group, the experimental group, Decoction Formula 1, Decoction Formula 2, Decoction Formula 3, Decoction Formula 4, Decoction Formula 5, Decoction Formula 6, Decoction Formula 7, and Decoction Formula 8, suggesting that the treatment group had a significant effect in alleviating contrast-induced acute kidney injury.

Claims

1. A Chinese medicine compound for alleviating contrast-induced acute renal injury, characterized in that It is prepared from the following raw materials in parts by weight: Astragalus 10-30; 4-8 pieces of processed rhubarb; Coptis chinensis 8-12; Plantago 1-20; Codonopsis pilosula 10-20; Psoralea corylifolia 10-20; Powdery mildew 8-12; Ligustrum lucidum 4-8; 2-4 grams of licorice.

2. A Chinese medicine complex for alleviating contrast-induced acute renal injury according to claim 1, characterized in that It is prepared from the following raw materials in parts by weight: Astragalus 20; Prepared rhubarb 6; Coptis chinensis 10; Plantago 15; Codonopsis pilosula 15; Psoralea corylifolia 15; Powdered Herba Dioscoreae 10; Ligustrum lucidum 6; Licorice 3.

3. A Chinese medicine complex for alleviating contrast-induced acute kidney injury according to claim 1, characterized in that: The dosage form of the traditional Chinese medicine complex is tablet, capsule, granule, pill, powder, oral liquid or aerosol.

4. A Chinese medicine complex for alleviating contrast-induced acute renal injury according to claim 1, characterized in that: The traditional Chinese medicine compound further comprises a pharmaceutically acceptable carrier.

5. The method for preparing a Chinese medicine complex for alleviating contrast-induced acute kidney injury according to claim 1, characterized in that: The steps include: 1) Weigh each Chinese medicine raw material according to weight; 2) mixing dextrin and water, stirring and dispersing, and preparing a dextrin aqueous solution; 3) a step of preparing a Chinese medicine extract, mixing and grinding astragalus, processed rhubarb, coptis root, plantain seed, codonopsis pilosula, psoralea corylifolia, radix ophiopogonis, fructus ligustris, and liquorice, adding 5-9 times the weight of deionized water, decocting 1-2 times, decocting for 1-2 hours each time, filtering, combining the filtrate, and concentrating under reduced pressure to obtain a water extract; 4) mixing the dextrin aqueous solution and the water extract in a mass ratio of 1:1, stirring, and preparing a Chinese medicine extract-dextrin aqueous solution mixture; mixing the Chinese medicine extract-dextrin aqueous solution mixture with auxiliary materials, and vacuum drying to prepare granules.

6. A Chinese medicine complex extract for alleviating contrast-induced acute renal injury, characterized in that: The extractant is prepared by extraction using the traditional Chinese medicine complex according to claim 1 or 2, wherein the extraction solvent of the extractant is water.

7. A Chinese medicine complex extract for alleviating contrast-induced acute renal injury according to claim 6, characterized in that: The extractant is a concentrated decoction or extract.

Images