Skin-improving cosmetic composition containing bakuchiol

Abstract

The present invention relates to a cosmetic composition and a pharmaceutical composition comprising bakuchiol, a silybum marianum fruit extract, and a myrobalan fruit extract; a skin improvement method; the bakuchiol, silybum marianum fruit extract, and myrobalan fruit extract of the present invention have an excellent skin improvement effect, and thus have high utilization rates in a skin improvement cosmetic composition and a drug composition, and uses thereof. Therefore, various industrial applications such as cosmetics and foreign medicines can be expected.

Description

Technical Field

[0001] The present invention relates to a cosmetic composition, a quasi-drug composition containing bakuchiol, an extract of silybum marianum fruits and an extract of terminalia chebula fruits; a method for improving skin; and uses thereof. Background Art

[0002] Including wrinkles, pigmentation, dryness, etc., which are called skin aging. Skin aging is divided into two types, there is aging caused by age and extrinsic aging caused by external factors such as ultraviolet A (UV-A), ultraviolet B (UV-B) and air pollution. It is known that UB-A is long-wavelength ultraviolet light (315 - 400 nm), which penetrates deeper into the skin than UV-B and causes damage to the dermis. The intermediate wavelength of ultraviolet light (UV-B) (280 - 315 nm) directly affects DNA, thereby damaging the epidermis. Among them, UV-B has higher energy than UV-A, so it is considered to be the main factor that destroys skin homeostasis. UV-B promotes the expression of matrix metalloproteinase-1 (MMP-1), which is the main collagen-degrading factor that degrades type I collagen, and helps the skin lose its tension and form wrinkles.

[0003] In addition, the above external factors such as cigarette smoke and ozone generate reactive oxygen species in skin cells, thereby damaging the skin. One of the results of generating reactive oxygen species is an increase in the level of Bcl-2 associated protein-x (Bax), leading to the activation of the charge-dependent anion channel of the cell membrane and a decrease in the mitochondrial membrane potential. In addition, Bax induces the release of cytochrome c in mitochondria, activates caspase-3, and thus causes cell death.

[0004] It is known that cosmetic ingredients usually cause various problems such as inflammation, acne, and swelling on the skin (Maibach.H.I., Contact Dermatitis, 6.369 - 404, 1980). In addition, various substances used as raw materials for functional cosmetics also have problems of low stability to the skin or little effect.

[0005] As an example, as a cosmetic for improving skin wrinkles and elasticity, adenosine, proteins derived from animal placenta, and extracts of chlorella are known. The effects of the most well-known extracts of chlorella are very small, and it is actually difficult to expect the effects of enhancing skin elasticity and improving wrinkles. Summary of the Invention

[0006] Technical Problem

[0007] As a result of the inventors of the present invention's unremitting efforts on substances showing excellent skin improvement effects, it was confirmed that bakuchiol, silybum marianum fruit extract, and terminalia chebula fruit extract have skin improvement effects, and have significantly excellent skin improvement effects compared with existing bakuchiol, thus completing the present invention.

[0008] Technical Solution

[0009] One object of the present invention is to provide a cosmetic composition containing bakuchiol, silybum marianum fruit extract, and terminalia chebula fruit extract.

[0010] Another object of the present invention is to provide a pharmaceutical external composition containing bakuchiol, silybum marianum fruit extract, and terminalia chebula fruit extract.

[0011] Yet another object of the present invention is to provide a method for improving skin, which includes the step of applying or administering a composition containing bakuchiol, silybum marianum fruit extract, and terminalia chebula fruit extract to the skin of an individual.

[0012] Still another object of the present invention is to provide the use of a composition containing bakuchiol, silybum marianum fruit extract, and terminalia chebula fruit extract for improving skin.

[0013] Advantages of the Invention

[0014] The bakuchiol, silybum marianum fruit extract, and terminalia chebula fruit extract of the present invention have excellent skin improvement effects, and thus have a high utilization rate in cosmetic compositions and pharmaceutical external compositions for skin improvement. Therefore, various industrial applications such as cosmetics and pharmaceutical external products can be expected. Description of the Drawings

[0015] Figure 1 It is a graph showing the RARG gene activity of silybum marianum fruit extract.

[0016] Figure 2 It is a graph showing the RAR activity of bakuchiol and silybum marianum fruit extract.

[0017] Figure 3 It is a result graph showing the human applicability evaluation results of bakuchiol, silybum marianum fruit extract, terminalia chebula fruit extract, mastic gum, and rosehip oil.

[0018] Figure 4 It is a result photo showing the human applicability evaluation results of bakuchiol, silybum marianum fruit extract, terminalia chebula fruit extract, mastic gum, and rosehip oil.

[0019] Figure 5 It is a 3D skin cross-section result showing the photoaging improvement effects of bakuchiol, silybum marianum fruit extract, terminalia chebula fruit extract, mastic gum, and rosehip oil.

[0020] Figure 6 It is a result diagram showing the effect of improving photoaging of bakuchiol, milk thistle fruit extract, terminalia chebula fruit extract, mastic gum, and rosehip oil.

[0021] Figure 7 It is a result diagram showing the effect of improving skin irritation and skin inflammation of bakuchiol, milk thistle fruit extract, terminalia chebula fruit extract, mastic gum, and rosehip oil. Detailed Description of the Invention

[0022] The following provides a specific description thereof. On the one hand, each description and embodiment disclosed in the present invention can also be applied to each different description and embodiment. That is, all combinations of various elements disclosed in the present invention fall within the scope of the present invention. In addition, it should not be considered that the scope of the present invention is limited by the specific description described below.

[0023] In addition, those of ordinary skill in the art can recognize or confirm most equivalents of specific aspects of the present invention described in the present invention only by using conventional experiments. In addition, these equivalents are intended to be included in the present invention.

[0024] One aspect of the present invention provides a cosmetic composition comprising bakuchiol, milk thistle fruit extract, and terminalia chebula fruit extract.

[0025] The term "bakuchiol" in the present invention, as a natural ingredient derived from plants, is a meroterpene of the terpenophenol series. It is known to have antioxidant activity as a material extracted from psoralea seeds. Bakuchiol has a gene expression pattern similar to that of retinoid in the skin, so it has begun to be mentioned as plant-based natural retinol, but these two substances have no structural similarity and there are differences in the mechanism of action. For bakuchiol, the expression and activity of retinoic acid receptors induced by retinol are low, and the efficacy of improving wrinkles is lower than that of retinol.

[0026] The bakuchiol of the present invention may have the following structure.

[0027] [Chemical Formula 1]

[0028]

[0029] In the present invention, there is no particular limitation on the method of obtaining bakuchiol, and substances collected from nature, chemically synthesized by methods known in the art, or commercially available can be used.

[0030] In addition, the above-mentioned bakuchiol may include all derivatives within the scope that can be envisioned by those skilled in the art in bakuchiol, and as long as it has the same effect in the present invention, it is included without limitation.

[0031] The term "Silybum Marianum, S.m" in the present invention refers to a Compositae plant that is also known as milk thistle. It is known that Silybum Marianum extract has traditionally been effective against various liver diseases such as hepatitis or alcoholic liver disease, and has thus been used in traditional medicine for centuries.

[0032] The term "Terminalia Chebula, medicinal terminalia fruit" in the present invention refers to the mature fruits of the deciduous trees Terminalia chebula or Terminalia chebula Retz. var. tomentella Kurt. belonging to the Combretaceae family, which are also known as Chebulae Fructus, Chebulae Fructus, Suifengzi, and Heli, and are used medicinally in Asia. In Korean medicine, it has astringent and mild medicinal properties, and is mostly used for the treatment of astringency, hemostasis, chronic pharyngitis, etc.

[0033] The above-mentioned plants may refer to specific parts such as the roots, leaves, flowers, stems, and fruits of Silybum Marianum or Terminalia Chebula, or may refer to the whole plant. In a specific embodiment, the composition of the present invention may contain fruit extracts of Silybum Marianum and Terminalia Chebula.

[0034] The term "extract" in the present invention refers to the liquid components obtained by impregnating the target substance into various solvents and then extracting for a certain period of time at normal temperature or under heating, and the resultant substances such as solid components obtained by removing the solvent from the above liquid components. The above extraction can be carried out more than once, and is not limited thereto. Moreover, it can be generally interpreted to include all of the filtrates, dilutions, concentrates, crude refined products, refined products, dried products, etc. of the above resultant substances. Thus, the Silybum Marianum fruit extract and Terminalia Chebula fruit extract provided in the present invention can be interpreted to include the extraction liquid obtained by extracting Silybum Marianum fruit or Terminalia Chebula fruit, the refined product obtained by enzymatically hydrolyzing the above extraction liquid, or a mixture thereof, etc., the extraction liquid itself and all dosage forms of extracts that can be formed using the extraction liquid, and / or their dried products.

[0035] The method for extracting the Silybum Marianum fruit extract and Terminalia Chebula fruit extract of the present invention is not particularly limited, and can be extracted according to the methods commonly used in the technical field. As non-limiting examples of the above extraction methods, hot water extraction method, ultrasonic extraction method, filtration method, reflux extraction method, etc. can be cited, and these can be carried out alone or in combination of two or more methods.

[0036] As the solvent used in the preparation of the above extract, water, alcohol, or a mixed solvent thereof can be exemplified. These can be used alone or in combination of two or more. When alcohol is used as the solvent, specifically, an alcohol having 1 to 4 carbon atoms, ethanol, methanol, or butanol can be used.

[0037] In the present invention, the above bakuchiol, silybum marianum fruit extract, and terminalia chebula fruit extract can be mixed in a weight ratio of 1:0.1:0.1 to 1:100:100, specifically 1:1:1 to 1:50:50, and more specifically 1:1:1 to 1:10:10.

[0038] In the present invention, "Retinoic Acid Receptor (RAR)" refers to a receptor that exists in the nucleus and is necessary for the action of retinoic acid, which can be called the active form of vitamin A. When retinoic acid is applied to the skin, the number of fibroblasts that synthesize collagen increases, and the synthesis of new collagen increases. The new collagen produced in fibroblasts accumulates at the site where the epidermis and dermis meet, ultimately regenerating the damaged dermis, improving wrinkles, and enhancing skin elasticity. It is known that retinoic acid has the effect of regenerating damaged elastic fibers in addition to the action of regenerating collagen fibers.

[0039] In the present invention, "Retinoic Acid Receptor Gamma (RARG, RARγ)", as a retinoic acid receptor gene, refers to a gene that requires the binding and increased activity of retinoic acid to produce reactions such as wrinkle improvement caused by retinol.

[0040] In the present invention, "ITGA6 (Integrin alpha 6)" is known to play a role in strengthening the skin barrier and is a gene that directly binds to laminin and plays an anti-aging role. This laminin connects and fixes the dermis-epidermis in the basement membrane between the dermis and epidermis, thereby imparting stability and maintaining the skin structure. In the present invention, "strengthening the skin barrier" can refer to all actions that enhance the function of the skin barrier located at the outermost periphery of the skin and prevent water and nutrient loss.

[0041] In one embodiment of the present invention, it was confirmed that when the silybum marianum fruit extract was treated, the expression of the RARG gene increased ( Figure 1 ), and it was confirmed that when bakuchiol and the silybum marianum fruit extract were treated together, the RAR activity increased compared to when only bakuchiol was treated ( Figure 2 ).

[0042] In another embodiment of the present invention, it was confirmed that when bakuchiol and Terminalia chebula fruit extract were treated together, the expression of the ITGA6 gene increased compared to when only bakuchiol was treated (Table 2).

[0043] In the present invention, the above cosmetic composition may additionally contain mastic gum or rosehip oil, but is not limited thereto.

[0044] The term "Mastic gum" of the present invention is obtained by drying the sap of the mastic tree and is known to have been used as a medicine for stomach health in Greece since ancient times.

[0045] The term "Rosehip oil" of the present invention refers to an oil extracted from the fruits or seeds of wild roses (Rosa canina). Rosehip oil is known to block moisture loss, thereby improving moisturizing power.

[0046] In the present invention, relative to 1% by weight of bakuchiol, the above mastic gum can be mixed in an amount of 0.0001 to 100% by weight, specifically 0.001 to 50% by weight, more specifically 0.01 to 10% by weight, and relative to 1% by weight of bakuchiol, rosehip oil can be mixed in an amount of 0.001 to 100% by weight, specifically 0.01 to 10% by weight, more specifically 0.1 to 5% by weight.

[0047] In one embodiment of the present invention, it was confirmed that when mastic gum and / or rosehip oil were additionally mixed in bakuchiol and Terminalia chebula fruit extract, the expression level of the ITGA6 gene increased compared to when bakuchiol and Terminalia chebula fruit extract were treated (Table 2).

[0048] In the present invention, the composition may have a skin-improving effect.

[0049] The term "skin improvement" of the present invention may be any one or more selected from the group consisting of improvement of wrinkles, improvement of elasticity, improvement of aging, improvement of skin irritation, and improvement of inflammation, but is not limited thereto.

[0050] The term "improvement" of the present invention refers to all actions that at least reduce parameters related to the alleviation or treatment of a condition, such as the degree of symptoms, and may include any one or more of improvement of wrinkles, improvement of elasticity, improvement of aging, improvement of skin irritation, and improvement of inflammation.

[0051] The above skin improvement can be achieved by the effects of activating the retinoic acid receptor, increasing the expression of the retinoic acid receptor gamma gene, increasing the expression of the ITGA6 gene, promoting collagen synthesis, or reducing the expression of inflammatory substances, but is not limited thereto.

[0052] Specifically, the inventors of the present invention have identified bakuchiol, milk thistle fruit extract, and chebula fruit extract; the composition containing frankincense gum and / or rosehip oil additionally in the above mixture has excellent skin improvement effects. More specifically, it has been confirmed that bakuchiol, milk thistle fruit extract, and chebula fruit extract; the composition containing frankincense gum and / or rosehip oil additionally in the above mixture has excellent effects such as activating retinoic acid receptor, increasing the expression of retinoic acid receptor gamma gene, increasing the expression of ITGA6 gene, promoting collagen synthesis, or reducing the expression of inflammatory substances. Thus, it has been confirmed that bakuchiol, milk thistle fruit extract, and chebula fruit extract; the composition containing frankincense gum and / or rosehip oil additionally in the above mixture has excellent skin improvement effects such as improving wrinkles, improving elasticity, improving aging, improving skin irritation, and improving inflammation.

[0053] The term "wrinkle" in the present invention can generally be divided into expression wrinkles caused by mimetic muscles for making facial expressions and fine lines caused by overall or local skin aging. Specifically, wrinkles can be induced by genetic causes, reduction of collagen and elastic fibers present in the dermis of the skin, external environment, etc. For the purpose of the present invention, "wrinkle" can be used to mean including all expression wrinkles or fine lines, and is not limited thereto.

[0054] The term "elasticity" in the present invention refers to the force that keeps the skin taut when pressed with a finger, and "improving elasticity" or "enhancing elasticity" refers to improving the elasticity of the skin by strengthening the structure of the subcutaneous fat layer of an individual, and can refer to improving the elasticity of the skin by using the composition of the present invention.

[0055] The term "Collagen" in the present invention is a fibrin that is common in most animals, especially mammals, and accounts for most of all connective tissues in the body such as skin and cartilage. The most common cells in the body, fibroblasts, produce and secrete collagen. Gelatin, which is widely used in cooking, or the food and pharmaceutical industries, is obtained by irreversibly hydrolyzing collagen. The reduction of collagen is the main cause of inducing wrinkles and reducing skin elasticity, so the synthesis of collagen is essential for improving wrinkles and enhancing elasticity.

[0056] In one embodiment of the present invention, it was confirmed that when treating bakuchiol, silybum marianum fruit extract, and terminalia chebula fruit extract, the amount of collagen synthesis was significantly increased compared to when treating bakuchiol alone. Additionally, it was confirmed that when using a mixture of bakuchiol, silybum marianum fruit extract, terminalia chebula fruit extract, gum mastic, and / or rosehip oil, the amount of collagen synthesis was further increased (Table 3).

[0057] In another embodiment of the present invention, the results of applying a dosage form containing bakuchiol, silybum marianum fruit extract, terminalia chebula fruit extract, gum mastic, and rosehip oil to the human body confirmed that the crow's feet, nasolabial folds, and cheek elasticity were improved ( Figure 3 , Figure 4 ).

[0058] The term "aging" in the present invention refers to the phenomenon of the decline of the structure and function of an organism over time. The prevention of aging in the present invention is not limited to its degree, etc., as long as aging is improved (for example, preventing, inhibiting, delaying aging, etc.). In the present invention, improving wrinkles and preventing aging can be attributed to promoting collagen synthesis, but it is not limited thereto.

[0059] Photoaging, which is the biggest cause of extrinsic aging, is caused by UV. UV can be divided into UVA, UVB, and UVC according to wavelength. UVB (ultraviolet-B) has the greatest impact on the skin and mainly affects skin cells by penetrating into the epidermis. UVA (ultraviolet-A) penetrates into the dermis of the skin and mainly affects skin cells related to collagen synthesis. The formation of wrinkles, which is a representative phenomenon of skin aging, is mainly due to the reduction of collagen in the dermis tissue. Dermal fibroblasts in the dermis layer regulate the expression of collagen, elastin, and MMP. UV irradiation reduces the synthesis of structural proteins such as collagen or elastin and increases the expression of MMP.

[0060] The term "photoaging" in the present invention refers to the phenomenon of skin damage such as a decrease in skin elasticity and moisture and the formation of wrinkles when exposed repeatedly or for a long time to the ultraviolet rays in sunlight.

[0061] In one embodiment of the present invention, it was confirmed that when treating bakuchiol, silybum marianum fruit extract, terminalia chebula fruit extract, gum mastic, and rosehip oil, the skin barrier strengthening effect was excellent, and the cell protection effect against UV to prevent collagen decomposition was excellent, resulting in a significant increase in the photoaging improvement effect ( Figure 5 , Figure 6 ).

[0062] The term "improving skin irritation" in the present invention refers to alleviating and subsiding erythema, itching, or irritated skin areas, etc., and can include the concept of relieving skin irritation. For example, relieving pruritus cutis, relieving cutaneous algesia, reducing water loss of the skin, and / or reducing flushing can be cited, but are not limited thereto.

[0063] The term "inflammation" in the present invention is a biological reaction in the body to damage or infection of a specific tissue, and the main medium is immune cells. The purpose of this inflammation is to maximally inhibit tissue damage, remove the infective agent, and regenerate the tissue. In the present invention, improvement, sedation, and regeneration of skin irritation can be attributed to inhibiting inflammatory substances (IL-1α), but are not limited thereto.

[0064] Retinol or bakuchiol is known as an inflammatory factor-stimulating inducer, and there are problems such as causing flushing, irritation, and inflammation (British Journal of Dermatology (2019) 180, pages 289-296).

[0065] Therefore, in one embodiment of the present invention, it was also confirmed that when bakuchiol was treated alone, the irritation did not decrease but increased. On the contrary, when bakuchiol, milk thistle fruit extract, terminalia chebula fruit extract, mastic gum, and rosehip oil were treated, the expression of the inflammatory factor IL-1α induced by the irritation factor was reduced ( Figure 7 ).

[0066] The term "cosmetic composition" in the present invention can be a dosage form selected from the group consisting of solutions, topical ointments, creams, foams, nutritional lotions, softeners, perfumes, face masks, soft waters, emulsions, makeup primers, essences, soaps, liquid cleansers, bath salts, sunscreens, sunscreen oils, suspensions, emulsions, pastes, gels, lotions, powders, soaps, surfactant-containing cleaning agents, oils, powder foundations, emulsion foundations, wax foundations, patches, and sprays, but is not limited thereto.

[0067] The cosmetic composition of the present invention may additionally contain one or more cosmetically acceptable carriers incorporated in ordinary skin cosmetics. As conventional ingredients, for example, oil, water, surfactants, humectants, lower alcohols, thickeners, chelating agents, pigments, preservatives, fragrances, etc. can be appropriately incorporated, but are not limited thereto.

[0068] The cosmetically acceptable carriers contained in the cosmetic composition of the present invention vary depending on the dosage form of the above cosmetic composition.

[0069] When the cosmetic dosage form of the present invention is an ointment, paste, cream or gel, as the carrier component, animal oil, vegetable oil, wax, paraffin, starch, tragacanth, cellulose derivatives, polyethylene glycol, silicone, bentonite, silica, talc, zinc oxide, etc. can be used, but not limited thereto. These can be used alone or in combination of two or more.

[0070] When the dosage form of the present invention is a solution or emulsion, as the carrier component, a solvent, solubilizer or emulsifier, etc. can be used. For example, water, ethanol, isopropyl alcohol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1,3-butylene glycol oil, etc. can be used. In particular, cottonseed oil, peanut oil, corn germ oil, olive oil, castor oil and sesame oil, glycerol fatty acid esters, polyethylene glycol or fatty acid esters of sorbitan can be used, but not limited thereto. These can be used alone or in combination of two or more.

[0071] When the dosage form of the present invention is a suspension, as the carrier component, a liquid diluent such as water, ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitan ester and polyoxyethylene sorbitan anhydride, microcrystalline cellulose, aluminum metahydroxide, bentonite, agar or tragacanth, etc. can be used, but not limited thereto. These can be used alone or in combination of two or more.

[0072] When the dosage form of the present invention is a soap, as the carrier component, alkali metal salts of fatty acids, fatty acid semi-esters, fatty acid protein hydrolysates, hydroxyethyl sulfonates, lanolin derivatives, fatty alcohols, vegetable oils, glycerol, sugars, etc. can be used, but not limited thereto. These can be used alone or in combination of two or more.

[0073] When the dosage form of the present invention is a powder or spray, as the carrier component, lactose, talc, silica, aluminum hydroxide, calcium silicate, polyamide powder or their mixture can be used. In particular, when it is a spray, a propellant such as chlorofluorocarbon, propane / butane or dimethyl ether can be additionally included.

[0074] On the one hand, all components described in the present invention can preferably be included in the composition of the present invention within the range not exceeding the maximum use values specified in relevant regulations such as cosmetic safety standards and the "Cosmetic Safety and Technical Specifications" in China.

[0075] Another aspect of the present invention provides a pharmaceutical external composition containing bakuchiol, silybum marianum fruit extract and terminalia chebula fruit extract.

[0076] The above-mentioned "bakuchiol", "silybum marianum fruit extract" and "terminalia chebula fruit extract" are as described above.

[0077] In the present invention, the composition can have a skin-improving effect.

[0078] The above-mentioned "skin improvement" is as described above.

[0079] In the present invention, the pharmaceutical external preparation composition may additionally contain gum olibanum or rosehip oil, but is not limited thereto.

[0080] The above-mentioned "gum olibanum" and "rosehip oil" are as described above.

[0081] The term "pharmaceutical external preparation" of the present invention refers to an article that is used for the purpose of diagnosing, treating, improving, alleviating, treating, or preventing diseases of humans or animals and has a milder effect than pharmaceuticals. For example, articles excluded as pharmaceuticals under the Pharmaceutical Affairs Law, including products for treating or preventing diseases of humans / animals, products that have a mild effect on the human body or do not act directly, etc.

[0082] Specifically, the above-mentioned pharmaceutical external preparation may include skin external preparations and personal hygiene products. More specifically, it may be a disinfectant cleaner, bath foam, mouthwash, wet tissue, washing soap, hand sanitizer, or ointment, but is not limited thereto.

[0083] When the above-mentioned composition of the present invention is used as a pharmaceutical external preparation additive, the above-mentioned composition can be directly added or used together with other pharmaceutical external preparations or pharmaceutical external preparation ingredients, and can be appropriately used according to conventional methods. The mixing amount of the active ingredient can be appropriately determined according to the purpose of use.

[0084] Another aspect of the present invention provides a method for improving skin, which includes the step of applying or administering a composition containing bakuchiol, silybum marianum fruit extract, and terminalia chebula fruit extract to the skin of an individual.

[0085] The above-mentioned composition may additionally contain gum olibanum or rosehip oil, but is not limited thereto.

[0086] The above-mentioned composition may be a cosmetic or pharmaceutical external preparation composition, but is not limited thereto.

[0087] The above-mentioned "bakuchiol", "silybum marianum fruit extract", "terminalia chebula fruit extract", "cosmetic composition", "gum olibanum", and "rosehip oil" are as described above.

[0088] The term "individual" of the present invention refers to all animals including humans that need to improve skin wrinkles, improve elasticity, improve aging, improve irritation or inflammation, or have insufficient collagen, or have erythema, pruritus, or irritated skin. The above-mentioned animals can not only be humans, but also mammals such as cows, horses, sheep, pigs, goats, camels, antelopes, dogs, and cats that need to treat the above-mentioned similar symptoms, but are not limited thereto.

[0089] In the present invention, the term "coating" refers to all methods of bringing the composition of the present invention into contact with an individual's skin by any suitable method, with the aim of absorbing the composition into the skin thereby.

[0090] When the composition of the present invention is coated on an individual's skin, it can have the effects of improving wrinkles, improving elasticity, improving aging, improving skin irritation, and improving inflammation, and may include the step of coating or administering the above composition to an individual in an effective amount showing the above effects.

[0091] In the present invention, the term "administration" refers to the general term for providing a given substance to an individual by any suitable method, and the above administration route can be administered by any conventional route as long as it can reach the target skin.

[0092] Another aspect of the present invention provides the use of a composition containing bakuchiol, silybum marianum fruit extract, and terminalia chebula fruit extract for skin improvement.

[0093] The above composition may additionally contain mastic gum or rosehip oil, but is not limited thereto.

[0094] The above composition may be a cosmetic or pharmaceutical external composition, but is not limited thereto.

[0095] The above "bakuchiol", "silybum marianum fruit extract", "terminalia chebula fruit extract", "cosmetic composition", "skin improvement", "mastic gum", "rosehip oil" are as described above.

[0096] Embodiments

[0097] The present invention will be described in more detail below by way of examples and experimental examples. However, these examples and experimental examples are used to illustrate the present invention exemplarily, and the scope of the present invention is not limited to these examples and experimental examples.

[0098] Experimental Example 1. Preparation of Samples

[0099] The bakuchiol purchased and used in the present invention is Sytenol A (raw material name) of sytheon company, the silybum marianum fruit extract is Silymarin phytosome (raw material name) of Indena company, the terminalia chebula fruit extract is Synastol TC (raw material name) of sytheon company, the rosehip oil is ROSEHIPOIL REFINED (raw material name) of TEXTRON TECNICA company, and the mastic gum is Novoretin (raw material name) of Mibelle company.

[0100] Example 1. Evaluation of the gene activities of retinoic acid receptor gamma (RARG) and retinoic acid receptor (RAR) of bakuchiol and silybum marianum fruit extract Example 1-1. Evaluation of the RARG gene activity of silybum marianum fruit extract

[0101] Figure 1

[0102] The Silybum Marianum Fruit Extract utilized a material containing 17.9% silymarin as the index component. To measure the expression level of the retinoic acid receptor gene in the nucleus, namely RARG, human dermal fibroblasts were dispensed into a 6-well plate at 5*10 5 cells / well and cultured overnight in a CO 2 incubator. Substances were added to the culture medium at different concentrations and then cultured for 24 hours. RNA was extracted using the RNA-spin Total RNA Extraction Kit (RNA-spin Total RNA Extraction Kit, Intron Biotechnology, Korea) according to the protocol, and real-time qPCR was performed using Taqman probes to comparatively analyze the RARG gene expression level of the Silybum Marianum Fruit Extract, and then the measurement results ( Example 1-2. Evaluation of the RAR activity of bakuchiol and silybum marianum fruit extract ) were confirmed.

[0103] As a result, it was confirmed that the expression of the RARG gene increased when treated with the Silybum Marianum Fruit Extract.

[0104] Figure 2

[0105] Bakuchiol (BK) and the Silybum Marianum Fruit Extract (SM) were mixed at the concentrations shown in Table 1 below in DMEM medium (Dulbecco Modified Eagle Medium) at their respective different concentrations and then the cells were treated. Specifically, to measure the degree of RAR activity caused by retinoic acid converted from retinol entering the nucleus and binding to the retinoic acid receptor (RAR), the RARγ Reporter (Luc)-HEK293 cell line (RARγ Reporter (Luc)-HEK293 cell line, BPS bioscience, USA) was purchased and an experiment was conducted. The cells were dispensed into a 96-well plate at 10 4 cells / well and cultured overnight in a CO 2 incubator. Bakuchiol (BK) and the Silybum Marianum Fruit Extract (SM) were added to the culture medium at different concentrations and then cultured for 24 hours. After the culture ended, the Steady-Glo(R) Luciferase Assay System (Steady-Glo(R) Luciferase Assay System, Promega, USA) was used to compare the differences in the activity levels of the retinoic acid receptor using luciferase analysis (SM 10ppm )。

[0106] [Table 1]

[0107] 0 BK 0.1ppm 0 1 1.60 BK 1ppm 0.96 1.71 BK 5ppm 0.94 1.62 Example 2. Evaluation of the ITGA6 gene expression levels of bakuchiol, terminalia chebula fruit extract, boswellia serrata gum, and rosehip oil 0.88 1.67

[0108] As a result, it was found that when silybum marianum fruit extract at 10 ppm was treated together with bakuchiol, compared with the control group, the RAR activity was promoted by about 1.6 times or more, and it was confirmed that the RAR activity increased compared with when silybum marianum fruit extract was treated alone.

[0109] From this, it was confirmed that silybum marianum fruit extract increased the RAR activity compared with bakuchiol alone, and thus it was found that silybum marianum fruit extract is excellent as a material for increasing the RAR activity.

[0110] Example 3. Confirmation of the improvement effects on wrinkles or elasticity of bakuchiol, silybum marianum fruit extract, terminalia chebula fruit extract, boswellia serrata gum, and rosehip oil

[0111] Bakuchiol, terminalia chebula fruit extract, mastic gum, and rosehip oil were mixed at the concentrations shown in Table 2 below in DMEM medium at their respective different concentrations, and then the cells were treated. Specifically, in order to measure the expression level of the ITGA6 gene related to the barrier function, the keratinocyte HaCaT was dispensed into a 6-well plate at 5*10 5 cells / well and cultured overnight in a CO 2 incubator. The substances were added to the culture medium at different concentrations and then cultured for 24 hours. Total RNA was extracted using the RNA-spin Total RNA Extraction Kit (RNA-spin Total RNA Extraction Kit, Intron Biotechnology, Korea) according to the protocol, and real-time qPCR (Real-time qPCR) was performed using Taqman probes to compare and analyze the expression level of the ITGA6 gene, and then the measurement results were confirmed (Table 2).

[0112] [Table 2]

[0113]

[0114] As a result, it was confirmed that when bakuchiol was treated together with terminalia chebula fruit extract; bakuchiol and mastic gum; bakuchiol, terminalia chebula fruit extract, and mastic gum; bakuchiol, terminalia chebula fruit extract, mastic gum, and rosehip oil, the expression level of the ITGA6 gene increased significantly compared with when bakuchiol was treated alone. In particular, it was confirmed that when bakuchiol and terminalia chebula fruit extract were treated, the synergistic effect brought about by terminalia chebula fruit extract led to an increase in the ITGA6 gene expression. It was confirmed that when bakuchiol, terminalia chebula fruit extract, mastic gum, and rosehip oil were treated, the expression level of the ITGA6 gene was the most excellent.

[0115] It can be seen therefrom that when bakuchiol is applied with Terminalia chebula fruit extract, ghatti gum or rosehip oil, the expression of the bakuchiol-specific gene ITGA6 increases, and it can be seen that the function of the skin barrier is enhanced.

[0116] Example 3-1. Evaluation of the enhanced collagen synthesis of bakuchiol, silybum marianum fruit extract, terminalia chebula fruit extract, boswellia serrata gum, and rosehip oil Example 3-2. Evaluation of the human applicability of bakuchiol, silybum marianum fruit extract, terminalia chebula fruit extract, boswellia serrata gum, and rosehip oil

[0117] Figure 3 Figure 4

[0118] Bakuchiol, silybum marianum fruit extract, Terminalia chebula fruit extract, ghatti gum and rosehip oil were mixed at the concentrations shown in Table 3 below in DMEM medium at their respective different concentrations, and then the cells were treated. Specifically, to evaluate the collagen biosynthesis ability, first, human skin fibroblasts were dispensed into 24-well plates at 2*10 4 cells / well and cultured overnight in a CO 2 incubator. After removing the culture medium from each well, it was gently washed with PBS, and after replacing each well with the culture medium again, the substances were added according to the recorded concentrations and combinations, and then cultured for 24 hours. Then, the culture medium was obtained, and procollagen secreted into the culture medium was measured using a procollagen type C-peptide EIA kit (MK-101, Takara Bio, Japan).

[0119] [Table 3]

[0120]

[0121] As a result, it was confirmed that when silybum marianum fruit extract or Terminalia chebula fruit extract was mixed with bakuchiol, the amount of collagen synthesis increased slightly compared to when bakuchiol, silybum marianum fruit extract or Terminalia chebula fruit extract was treated alone.

[0122] However, it was confirmed that when bakuchiol, silybum marianum fruit extract and Terminalia chebula fruit extract were mixed and treated, the amount of collagen synthesis increased by about 85% or more compared to no treatment, and it was found that there was a synergistic effect when silybum marianum fruit extract and Terminalia chebula fruit extract were mixed with bakuchiol. In particular, it was confirmed that when ghatti gum and / or rosehip oil was additionally used, the amount of collagen synthesis further increased.

[0123] It can be seen from this that the bakuchiol, the fruit extract of Silybum marianum, and the fruit extract of Terminalia chebula; the combination of bakuchiol, the fruit extract of Silybum marianum, the fruit extract of Terminalia chebula, and mastic gum; the combination of bakuchiol, the fruit extract of Silybum marianum, the fruit extract of Terminalia chebula, and rosehip oil; and the combination of bakuchiol, the fruit extract of Silybum marianum, the fruit extract of Terminalia chebula, mastic gum, and rosehip oil show excellent effects in improving skin wrinkles and enhancing elasticity, etc.

[0124] Example 4. Confirmation of the improvement effects on photoaging, skin irritation, and skin inflammation of bakuchiol, silybum marianum fruit extract, terminalia chebula fruit extract, boswellia serrata gum, and rosehip oil Example 4-1. Confirmation of the photoaging improvement effect of bakuchiol, silybum marianum fruit extract, terminalia chebula fruit extract, boswellia serrata gum, and rosehip oil

[0125] Prepared a formulation containing 0.1% bakuchiol, 0.5% Silymarin phytosome (fruit extract of Silybum marianum), 0.5% Synastol TC (fruit extract of Terminalia chebula), 0.1% Novoretin (mastic gum), and 0.01% Rosehipoil (rosehip oil) (4 kinds of enhancers); a cream formulation containing 0.1% bakuchiol as a control group. A total of 12 female subjects outside the institute aged 40 - 50 were used as subjects, and it was applied twice a day (once in the morning and once in the evening) to the area under the eyes, nasolabial folds, and cheeks for a total of 4 weeks. For the area under the eyes and nasolabial folds, Antera 3D was used, and for the elasticity of the cheeks, Cutometer was used to measure the wrinkle improvement rate, and the overall average wrinkle improvement rate of the subjects and the improvement graph of a representative subject were shown ( Figure 5 、 Figure 6 ).

[0126] As a result, it was confirmed that when treated with bakuchiol, the fruit extract of Silybum marianum, the fruit extract of Terminalia chebula, mastic gum, and rosehip oil, compared with when treated with bakuchiol alone, the improvement of wrinkles under the eyes was about 2.2 times, the nasolabial folds were about 2.6 times, and the elasticity of the cheeks was more than about 8 times.

[0127] In addition, it was confirmed that the improvement rate of wrinkles under the eyes increased by about 5.3 times, and when treated with bakuchiol, the fruit extract of Silybum marianum, the fruit extract of Terminalia chebula, mastic gum, and rosehip oil, the depth and length of the nasolabial folds decreased by about 3.1 times.

[0128] It can be seen from this that the bakuchiol, the fruit extract of Silybum marianum, the fruit extract of Terminalia chebula, mastic gum, and rosehip oil of the present invention have excellent effects in improving wrinkles and elasticity.

[0129] Figure 5 Example 4-2. Confirmation of the improvement effects on skin irritation and skin inflammation of bakuchiol, silybum marianum fruit extract, terminalia chebula fruit extract, boswellia serrata gum, and rosehip oil

[0130] Figure 7 ​

[0131] Applied to tegoscience, a 3D human skin model (3D reconstruct human skin) composed of the epidermis and dermis -ED, overnight treatment with bakuchiol and extracts of fruits of Silybum marianum, fruits of Terminalia chebula, Boswellia serrata gum, and Rosa canina oil (4 kinds of enhancers); bakuchiol and retinol as control groups, and the conditions for UV irradiation were: treated 2 times a day at 50 mJ / cm 2 with UVB for a total of 3 days and then cultured. After the culture was completed, paraffin blocks were made and stained with Masson's trichrome to confirm the 3D skin cross-section ( ​ , red: epidermis, blue: dermal collagen). Subsequently, the area of dermal collagen was measured using the publicly available software Image J ( ​ ).

[0132] As a result, it was confirmed that when irradiated with UVB repeatedly, the area of dermal collagen decreased by about 24% compared to the untreated group, indicating that the dermis was damaged by UV. It was confirmed that when treated with 0.1% retinol or 0.5% bakuchiol, the collagen area recovered by about 15%. As ​ shown, it was confirmed that in terms of the epidermal barrier protection effect, bakuchiol was superior to retinol. However, it was confirmed that even when the concentration of bakuchiol was increased to 1%, the efficacy of dermal collagen recovery did not increase further but decreased instead.

[0133] It was confirmed that when treated with the bakuchiol, extracts of fruits of Silybum marianum, fruits of Terminalia chebula, Boswellia serrata gum, and Rosa canina oil of the present invention, the epidermal barrier protection effect was excellent, and the ability to recover the area of dermal collagen was significantly improved compared to when treated with retinol or bakuchiol alone.

[0134] Thus, it can be seen that the bakuchiol, extracts of fruits of Silybum marianum, fruits of Terminalia chebula, Boswellia serrata gum, and Rosa canina oil of the present invention have excellent effects not only on improving wrinkles and elasticity but also on improving photoaging.

[0135] ​ ​

[0136] According to the same method as in Example 4-1, the extract was treated on the 3D human skin model and UVB was treated, followed by culturing. Then the culture medium was obtained, and the amount of the secreted inflammatory substance (IL-1α) was measured using a Human IL-1alpha / IL-1F1 DuoSet ELISA kit ( ​ ).

[0137] As a result, it was confirmed that when skin cells were stimulated by UV, IL-1a increased, and when treated with bakuchiol, which is known as a stimulant inducer of inflammatory factors, the stimulation did not decrease but increased. On the contrary, it was confirmed that bakuchiol, silybum marianum fruit extract, terminalia chebula fruit extract, boswellia serrata gum, and rosehip oil of the present invention reduced the expression of the inflammatory factor IL-1a induced by stimulatory factors.

[0138] From this, it can be seen that when bakuchiol is treated alone, it will instead stimulate the skin or induce inflammation, but when bakuchiol, silybum marianum fruit extract, terminalia chebula fruit extract, boswellia serrata gum, and rosehip oil of the present invention are treated together, the expression of inflammatory factors is reduced, and the effects of improving skin irritation and inflammation are excellent.

[0139] Based on the above description, those skilled in the art to which the present invention pertains can understand that the present invention can also be implemented in other specific ways without changing the technical idea or essential features of the present invention. In this regard, it should be understood that the embodiments described above are exemplary in all respects and not restrictive. The scope of the present invention should be interpreted as including the meaning and scope of the claims described below and all changes or variations derived from their equivalent concepts, rather than including only the above detailed description.

Claims

1. A cosmetic composition comprising bakuchiol, silybum marianum fruit extract, and terminalia chebula fruit extract.

2. The cosmetic composition according to claim 1, further comprising mastic gum or rosehip oil.

3. The cosmetic composition according to claim 1, wherein, the cosmetic composition is for improving the skin.

4. The cosmetic composition for skin improvement according to claim 3, wherein, the skin improvement is any one or more selected from the group consisting of improving wrinkles, improving elasticity, improving aging, improving skin irritation, and improving inflammation.

5. The cosmetic composition according to claim 4, wherein, the cosmetic composition has the effects of activating retinoic acid receptor, increasing the gene expression of retinoic acid receptor gamma, increasing the gene expression of ITGA6, promoting collagen synthesis, or reducing the expression of inflammatory substances.

6. The cosmetic composition according to claim 1, characterized in that, the bakuchiol, silybum marianum fruit extract, and terminalia chebula fruit extract are mixed in a weight ratio of 1:0.1:0.1 to 1:100:

100.

7. A pharmaceutical external preparation composition comprising bakuchiol, silybum marianum fruit extract, and terminalia chebula fruit extract.

8. The pharmaceutical external preparation composition according to claim 7, further comprising mastic gum or rosehip oil.

9. A method for skin improvement, comprising the step of applying or administering a composition comprising bakuchiol, silybum marianum fruit extract, and terminalia chebula fruit extract to an individual.

10. The method for skin improvement according to claim 9, wherein, the composition is a cosmetic or pharmaceutical external preparation composition.

11. Use of a composition comprising bakuchiol, silybum marianum fruit extract, and terminalia chebula fruit extract for skin improvement.

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