Medicinal and edible composition as well as preparation method and application thereof

Abstract

According to the medicine and food homologous composition and the preparation method and application thereof, in a hypoxia fatigue swimming experiment, the composition can reduce lactic acid accumulation, uric acid and urea nitrogen generation and exert the hypoxia and fatigue resisting effect, and it is proved through a hypoxia pulmonary vascular injury mouse model that the composition can reduce inflammatory response and regulate vascular homeostasis, and the anti-hypoxia and anti-fatigue effects are achieved. Therefore, the medicinal and edible composition can be used for preparing functional foods, health care products or medicines for resisting hypoxia fatigue and hypoxia pulmonary vascular injury.

Description

Technical Field

[0001] The present invention belongs to the field of functional foods, and relates to a composition of medicated and edible homologous substances, a preparation method thereof, and an application thereof. Background Art

[0002] In a special environment with high altitude, high cold, low pressure, and low oxygen, people who live or rapidly enter the plateau are extremely prone to altitude reactions such as poor acclimatization or metabolic disorders, which may cause altitude sickness and damage to the body. When people from the plain enter the plateau with poor acclimatization, they will experience symptoms such as headache, insomnia, fatigue, palpitations, shortness of breath, loss of appetite, and indigestion. After 3 - 10 days of acclimatization, the symptoms gradually disappear. However, due to the reduced oxygen supply, when training or related tasks require high-intensity exercise, the exercise tolerance of the human body decreases, and symptoms such as fatigue and shortness of breath will reappear, and even damage to lung function may be caused. Hypoxic pulmonary vascular injury is caused by hemodynamic disorders and inflammatory reactions caused by hypoxia, which increase pulmonary vascular resistance and lead to pulmonary vascular remodeling. Hypoxic pulmonary vascular injury is an important pathogenesis of various plateau pulmonary diseases. If not intervened and treated in time, it will further develop into high altitude pulmonary edema and high altitude pulmonary hypertension. Hypoxic pulmonary vascular injury caused by plateau hypoxia plays an important role in the occurrence and development of plateau pulmonary diseases. Therefore, in order to achieve rapid acclimatization to the plateau, improve the working ability of people rapidly entering the plateau, and break through the "last mile" of working in the plateau, it is of great significance to develop a safe and directly edible product composed of traditional medicated and edible homologous substances or plateau-exclusive medicinal plants, which can effectively resist altitude hypoxia, relieve hypoxic fatigue, and improve hypoxic pulmonary vascular injury.

[0003] At present, there are relatively many drugs and drug compositions related to anti-hypoxia in plateaus, but there are very few drug-food compositions for anti-hypoxic fatigue and anti-hypoxic pulmonary vascular damage in plateaus. The Chinese patent application with publication number CN113827631A discloses a Tibetan medicine compound for preventing and treating acute mountain sickness and its health care products, the formula contains 2-4 parts of genkwa root, 12-20 parts of sea buckthorn, and 10-15 parts of pomegranate seeds, and the instant tea prepared has a good preventive effect on acute mountain sickness and can effectively reduce the incidence of acute mountain sickness. The Chinese patent application with publication number CN113827631A discloses a genus arborvitae food with anti-fatigue effect and its preparation method, the formula includes 16.7-19.2 parts of genus arborvitae, 5.4-7.6 parts of epimedium powder, 3.5-5.2 parts of citronella, 8-10.5 parts of taurine, 1.2-2 parts of multivitamins, 2.4-3.3 parts of safflower, 3-4.5 parts of tuckahoe and 4-6.6 parts of polypeptide, which has good anti-fatigue effect. The Chinese patent application with publication number CN117815317A discloses a Chinese medicine formula for treating pulmonary hypertension, the formula includes 1-20 parts of vitex rotundifolia, 5-10 parts of dried tangerine peel, 8-20 parts of ginseng, 10-20 parts of licorice root, 10-20 parts of white peony root and 20-50 parts of astragalus, the formula effectively reduces pulmonary artery pressure, inhibits the occurrence of right heart hypertrophy and alleviates pulmonary edema. The formulas of these products are not entirely medicinal and edible substances, and they do not address the problems of reduced physical fitness and hypoxic pulmonary vascular damage that are prone to occur in the low-oxygen environment of the plateau. They can only be used to prepare medicines, and cannot be made into food and then put on the market on a large scale to solve the problem of efficiency in emergency plateau operations. Summary of the invention

[0004] In order to solve the above problems of the prior art, the present invention provides a medicine-food composition and a preparation method and application thereof. The medicine-food composition has the effects of relieving hypoxic fatigue and improving hypoxic pulmonary vascular damage.

[0005] The present invention is achieved through the following technical solutions: In a first aspect, the present invention provides a medicine-food composition, which comprises, by weight: 13.5-54 parts of genkwa root extract, 10-60 parts of seabuckthorn extract, and 3-30 parts of ginseng extract or ginseng powder.

[0006] Preferably, based on the weight percentage, it includes: 27 parts of genkwa root extract, 30 parts of seabuckthorn extract, and 3 parts of ginseng extract or ginseng powder.

[0007] Preferably, based on the weight percentage, it includes: 27 parts of genkwa root extract, 30 parts of seabuckthorn extract, and 3 parts of ginseng powder.

[0008] Second aspect, the present invention provides a preparation method of the above-mentioned medicine and food homology composition, which is characterized in that the radix raphani extract, seabuckthorn extract and ginseng extract or ginseng powder are taken and mixed to obtain the medicine and food homology composition.

[0009] Preferably, the preparation method of the radix raphani extract is as follows: take radix raphani, add water, soak until soft, bring to a boil over high heat, then turn to low heat and decoct. After the decoction is finished, keep the decocted liquid. The medicinal residues are decocted again with water, and the decoction is repeated several times. The decocted liquids are combined and filtered by suction, and concentrated under reduced pressure.

[0010] Preferably, the preparation method of the seabuckthorn extract is as follows: take seabuckthorn, add water, soak until soft, bring to a boil over high heat, then turn to low heat and decoct. After the decoction is finished, keep the decocted liquid. The medicinal residues are decocted again with water, and the decoction is repeated several times. The decocted liquids are combined and filtered by suction, and concentrated under reduced pressure.

[0011] Preferably, the preparation method of the ginseng extract is as follows: take ginseng, add water, soak until soft, bring to a boil over high heat, then turn to low heat and decoct. After the decoction is finished, keep the decocted liquid. The medicinal residues are decocted again with water, and the decoction is repeated several times. The decocted liquids are combined and filtered by suction, and concentrated under reduced pressure.

[0012] Preferably, the preparation method of the ginseng powder is as follows: dry the ginseng slices and then crush and sieve them.

[0013] Fourth aspect, the present invention provides the application of the above-mentioned medicine and food homology composition in the preparation of functional foods, health products or drugs for anti-hypoxic fatigue.

[0014] Fifth aspect, the present invention provides the application of the above-mentioned medicine and food homology composition in the preparation of functional foods, health products or drugs for treating hypoxic pulmonary vascular injury.

[0015] Compared with the prior art, the present invention has the following beneficial effects: The present invention first discovers a medicine and food homology composition composed of radix raphani extract, seabuckthorn extract and ginseng. In the hypoxic fatigue swimming experiment, it can reduce lactic acid accumulation, reduce the production of uric acid and urea nitrogen, and play an anti-hypoxic and anti-fatigue role. Using a mouse model of hypoxic pulmonary vascular injury, it is confirmed that this composition can reduce inflammatory responses, regulate vascular homeostasis, improve hemodynamic disorders, and inhibit vascular remodeling. Therefore, this medicine and food homology composition can be used to prepare functional foods, health products or drugs for anti-hypoxic fatigue and hypoxic pulmonary vascular injury.

[0016] Furthermore, through research, the present invention finds that the effect of ginseng powder is better than that of ginseng extract, and the addition amount of ginseng powder should not be too high. When its addition amount is relatively high, the effect of the composition will be reduced instead. Therefore, the optimal formula is: 27 parts of radix raphani extract, 30 parts of seabuckthorn extract, and 3 parts of ginseng powder.

[0017] The present invention confirms that the above-mentioned medicine and food homology composition has the effects of anti-hypoxia and anti-fatigue, can reduce inflammatory reactions, regulate vascular homeostasis, improve hemodynamic disorders, and inhibit vascular remodeling, and can be used to prepare functional foods, health products or drugs for anti-hypoxia fatigue and hypoxic pulmonary vascular injury. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] In order to more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the following will briefly introduce the drawings required for the description of the embodiments or the prior art. Obviously, the drawings in the following description are some embodiments of the present invention. For those of ordinary skill in the art, without creative efforts, other drawings can be obtained based on these drawings.

[0019] Figure 1 It is the anti-hypoxia effect of the medicine and food homology composition on mice with normobaric closed hypoxia. Among them, A is the survival time of mice with normobaric closed hypoxia; B is the increase rate of the survival time of mice with normobaric closed hypoxia. n±s ( n=10 ), *P<0.05, **P<0.01, ***P<0.001 vs. Mod group.

[0020] Figure 2 It is the serological indexes of the medicine and food homology composition to improve the anti-hypoxia ability of mice with normobaric closed hypoxia. Among them, A is the content of serum lactate dehydrogenase (LDH); B is the content of serum lactate (LACT); C is the content of serum glucose (GLUT); D is the content of serum uric acid (UA). n±s ( n=10 ), *P<0.05, **P<0.01, ***P<0.001 vs. Mod group.

[0021] Figure 3 It is the serological indexes of the medicine and food homology composition to improve the anti-hypoxia and anti-fatigue ability of mice with hypoxic swimming fatigue. Among them, A is the content of serum creatine kinase (CK); B is the content of serum urea (BUN); C is the content of serum lactate dehydrogenase; D is the content of serum lactate; E is the content of serum uric acid; F is the content of serum glucose. n±s ( n=10 ), *P<0.05, **P<0.01, ***P<0.001 vs. Con group, # P<0.05, ## P<0.01, ### P<0.001 vs. Mod group.

[0022] Figure 4To investigate the effects of the medicine-food homologous composition on improving inflammatory responses and permeability indexes in mice with hypoxic pulmonary vascular injury. Among them, A represents the numbers of white blood cells (WBC) (a), neutrophils (NEUT) (b), lymphocytes (LYMPH) (c), and basophils (BASO) (d) in serum; B represents the contents of tumor necrosis factor α (TNF-α) (a), interleukin-6 (IL-6) (b), interleukin 1β (IL-1β) (c), and interferon γ (IFN-γ) (d) in serum; C represents the numbers of white blood cells (a), neutrophils (b), lymphocytes (c), and monocytes (MONO) (d) in bronchoalveolar lavage fluid (BALF); D represents the contents of alkaline phosphatase (a) and lactate dehydrogenase (b) in serum; E represents the contents of alkaline phosphatase (a) and lactate dehydrogenase (b) in BALF. n±s ( n=10 ), *P < 0.05, **P < 0.01, ***P < 0.001, ***P < 0.0001 vs. Con group, # P < 0.05, ## P < 0.01, ### P < 0.001, #### P < 0.001 vs. Mod group.

[0023] Figure 5 To investigate the effects of the medicine-food homologous composition on inhibiting vascular remodeling indexes in mice with hypoxic pulmonary vascular injury. Among them, A represents cardiac output (CO(M-T eich)) (a), stroke volume (SV) (b), ejection fraction (EF) (c), and end-systolic volume (LVESV(M-T eich)) (d); B represents the percentage of wall area in total vascular area (a) and the percentage of arteriolar wall thickness in diameter (b). n±s ( n=10 ), *P < 0.05, **P < 0.01, ***P < 0.0001 vs. Con group, # P < 0.05, ## P < 0.01, ### P < 0.001, #### P < 0.001 vs. Mod group. Specific Embodiments

[0024] The following specific examples illustrate the embodiments of the present invention. Those skilled in the art can easily understand other advantages and effects of the present invention from the content disclosed in this specification. The present invention can also be implemented or applied through other different specific embodiments, and various details in this specification can also be modified or changed based on different viewpoints and applications without departing from the spirit of the present invention.

[0025] It should be noted that the process equipment or devices not specifically specified in the following embodiments are all conventional equipment or devices in the art.

[0026] It should be noted that the terms "include" and "have" and any variations thereof are intended to cover non-exclusive inclusions. For example, a process, method, system, product or device that includes a series of steps or units is not necessarily limited to those steps or units clearly listed, but may include other steps or units that are not clearly listed or inherent to these processes, methods, products or devices. Moreover, unless otherwise specified, the numbering of each method step is only a convenient tool for identifying each method step, and is not intended to limit the order of arrangement of each method step or to define the scope of the present invention. Changes or adjustments in their relative relationships should also be regarded as the scope of the present invention without substantially changing the technical content.

[0027] The edible-medicinal composition for resisting hypoxic fatigue and hypoxic pulmonary vascular injury of the present invention comprises, by weight, 13.5 to 54 parts of genkwa root extract, 10 to 60 parts of seabuckthorn extract, and 3 to 30 parts of ginseng extract or ginseng powder.

[0028] The Tibetan name of yew root is "Niuma", which is an ancient crop used as medicine, food and feed on the Qinghai-Tibet Plateau. According to the classic Tibetan medical work "Four Medical Classics", yew root is sweet and warm in nature, and the root tuber "expels wind, produces 'triba', nourishes, detoxifies, and treats 'bacon disease', 'long disease', and weakness", and has the effects of clearing heat and detoxifying, nourishing and increasing oxygen. Sea buckthorn is a common medicinal flavor in Chinese, Tibetan and Mongolian medicines, which has the effects of strengthening the spleen and digestion, relieving cough and removing phlegm, and promoting blood circulation and dispersing blood stasis; it is used for spleen deficiency, poor appetite, abdominal pain due to food accumulation, chest pain, heart pain, blood stasis and amenorrhea. Ginseng is a dual-purpose food and medicine substance, which has the effects of greatly replenishing vitality, tonifying the spleen and lungs, promoting body fluid and nourishing blood, calming the mind and increasing intelligence.

[0029] The present invention uses genkwa root extract, seabuckthorn extract and ginseng extract or ginseng powder to form a medicine-food combination. Studies have shown that the obtained combination has the effect of resisting hypoxic fatigue and treating hypoxic pulmonary vascular damage. The present invention uses a scientific and reasonable formula to reasonably match, and can play an anti-hypoxic fatigue and hypoxic pulmonary vascular damage effect from multiple targets; and compared with the currently commonly used drugs for resisting hypoxic fatigue and treating high-altitude lung diseases, the medicine-food combination can be used for drug, health care product and food formulation, and has higher safety for long-term use.

[0030] In a specific embodiment of the present invention, the formula of the medicine-food combination is: 13.5 parts of genkwa root extract, 15 parts of seabuckthorn extract, and 1.5 parts of ginseng extract. The extracts are mixed and administered at 1, 2, and 4 times respectively.

[0031] In another more preferred embodiment, the formula of the medicine-food composition is: 27 parts of genkwa root extract, 30 parts of seabuckthorn extract, and 3 parts of ginseng powder.

[0032] The radix raphani extract of the present invention is prepared from commercially available radix raphani dry products that meet the local standards of Qinghai Province. The preparation method is as follows: Take radix raphani, add water, soak until soft, bring to a boil over high heat, then turn to low heat and decoct. After the decoction is completed, retain the decoction liquid. Add water to the obtained medicinal residues again for decoction, repeat the decoction several times, combine the decoction liquids and perform suction filtration, and then concentrate under reduced pressure.

[0033] One specific embodiment includes: Take the original medicinal material radix raphani, add 15 times the volume of water, soak until soft, bring to a boil over high heat, then turn to low heat and decoct for 60 minutes. After completion, retain the decoction liquid. Add 10 times the volume of water to the medicinal residues again for decoction, decoct a total of 3 times, 1 hour each time. After the extract is suction filtered, concentrate under reduced pressure.

[0034] The seabuckthorn extract of the present invention is prepared from seabuckthorn dry products that meet the pharmacopoeia standards. The preparation method is as follows: Take seabuckthorn, add water, soak until soft, bring to a boil over high heat, then turn to low heat and decoct. After the decoction is completed, retain the decoction liquid. Add water to the obtained medicinal residues again for decoction, repeat the decoction several times, combine the decoction liquids and perform suction filtration, and then concentrate under reduced pressure.

[0035] One specific embodiment includes: Take the original medicinal material seabuckthorn, add 10 times the volume of water, soak until soft, bring to a boil over high heat, then turn to low heat and decoct for 60 minutes. After completion, retain the decoction liquid. Add 10 times the volume of water to the medicinal residues again for decoction, decoct a total of 3 times, 1 hour each time. After the extract is suction filtered, concentrate under reduced pressure.

[0036] The preparation method of the ginseng extract of the present invention is as follows: Take ginseng, add water, soak until soft, bring to a boil over high heat, then turn to low heat and decoct. After the decoction is completed, retain the decoction liquid. Add water to the obtained medicinal residues again for decoction, repeat the decoction several times, combine the decoction liquids and perform suction filtration, and then concentrate under reduced pressure.

[0037] One specific embodiment includes: Take the original medicinal material ginseng, add 10 times the volume of water, soak until soft, bring to a boil over high heat, then turn to low heat and decoct for 60 minutes. After completion, retain the decoction liquid. Add 10 times the volume of water to the medicinal residues again for decoction, decoct a total of 3 times, 1 hour each time. After the extract is suction filtered, concentrate under reduced pressure.

[0038] The ginseng powder of the present invention is prepared from ginseng that meets the pharmacopoeia standards. The preparation method is as follows: Dry the ginseng slices and then pulverize and sieve (for example, 300 mesh).

[0039] The preparation method of the medicine and food homologous composition of the present invention includes: Weigh and mix the above components according to weight.

[0040] The above medicine and food homologous composition can be used to prepare functional foods, health products or drugs for anti-hypoxia fatigue and hypoxic pulmonary vascular injury.

[0041] Example 1 This example provides a medicine-food homologous composition, including 13.5 parts of radix pseudostellariae extract, 15 parts of seabuckthorn extract, and 1.5 parts of ginseng extract.

[0042] The preparation method of the radix pseudostellariae extract is as follows: Take the original medicinal material radix pseudostellariae, add 15 times the volume of water, soak until soft, bring to a boil over high heat, then turn to low heat and decoct for 60 minutes. After that, retain the decoction. Add 10 times the volume of water to the medicinal residues and decoct again. Decoct a total of 3 times, each time for 1 hour. After the extract is filtered by suction, concentrate it under reduced pressure to 1 g / ml.

[0043] The preparation method of the seabuckthorn extract is as follows: Take the original medicinal material seabuckthorn, add 10 times the volume of water, soak until soft, bring to a boil over high heat, then turn to low heat and decoct for 60 minutes. After that, retain the decoction. Add 10 times the volume of water to the medicinal residues and decoct again. Decoct a total of 3 times, each time for 1 hour. After the extract is filtered by suction, concentrate it under reduced pressure to 1 g / ml.

[0044] The preparation method of the ginseng extract is as follows: Take the original medicinal material ginseng, add 10 times the volume of water, soak until soft, bring to a boil over high heat, then turn to low heat and decoct for 60 minutes. After that, retain the decoction. Add 10 times the volume of water to the medicinal residues and decoct again. Decoct a total of 3 times, each time for 1 hour. After the extract is filtered by suction, concentrate it under reduced pressure to 1 g / ml.

[0045] The preparation method of the medicine-food homologous composition is as follows: Weigh and mix the above components evenly to obtain the composition.

[0046] Example 2 This example provides a medicine-food homologous composition, including 27 parts of radix pseudostellariae extract, 30 parts of seabuckthorn extract, and 3 parts of ginseng powder.

[0047] The preparation method of the radix pseudostellariae extract is as follows: Take the original medicinal material radix pseudostellariae, add 15 times the volume of water, soak until soft, bring to a boil over high heat, then turn to low heat and decoct for 60 minutes. After that, retain the decoction. Add 10 times the volume of water to the medicinal residues and decoct again. Decoct a total of 3 times, each time for 1 hour. After the extract is filtered by suction, concentrate it under reduced pressure to 1 g / ml.

[0048] The preparation method of the seabuckthorn extract is as follows: Take the original medicinal material seabuckthorn, add 10 times the volume of water, soak until soft, bring to a boil over high heat, then turn to low heat and decoct for 60 minutes. After that, retain the decoction. Add 10 times the volume of water to the medicinal residues and decoct again. Decoct a total of 3 times, each time for 1 hour. After the extract is filtered by suction, concentrate it under reduced pressure to 1 g / ml.

[0049] The preparation method of the ginseng powder is as follows: Dry the ginseng slices and then crush them through a 300-mesh sieve.

[0050] The preparation method of the medicine-food homologous composition is as follows: Mix the radix pseudostellariae extract and the seabuckthorn extract evenly, then add the ginseng powder and mix evenly to obtain the composition.

[0051] Example 3 This embodiment provides a medicine and food homology composition, comprising 27 parts of radix raphani extract, 30 parts of seabuckthorn extract, and 10 parts of ginseng powder. The difference from Example 2 is that the proportion of ginseng powder is increased.

[0052] Its preparation method is the same as that of Example 2.

[0053] Example 4 This embodiment provides a medicine and food homology composition, comprising 27 parts of radix raphani extract, 30 parts of seabuckthorn extract, and 20 parts of ginseng powder. The difference from Example 2 is that the proportion of ginseng powder is increased.

[0054] Its preparation method is the same as that of Example 2.

[0055] Example 5 This embodiment provides a medicine and food homology composition, comprising 27 parts of radix raphani extract, 30 parts of seabuckthorn extract, and 30 parts of ginseng powder. The difference from Example 2 is that the proportion of ginseng powder is increased.

[0056] Its preparation method is the same as that of Example 2.

[0057] Experimental Example 1 Verification of the anti-hypoxia effect of the medicine and food homology composition on mice under normal pressure and airtight hypoxia (1) Method: 90 male KM mice (weighing 18 - 22 g) were housed separately in a clean, constant temperature (23 ± 2 °C) and constant humidity (55 ± 10%) animal room. The daily 12 h / 12 h day-night light cycle was adjusted artificially, and they had free access to water and food. After 3 days of adaptive feeding, the 90 mice were randomly grouped: (1) The model group (Mod, n = 10) was given saline by gavage daily; (2) The positive control group (Ac, n = 10) was given 100 mg / kg acetazolamide solution by gavage daily; (3) The mice in the Example 1 group (S1-1, S1-2, S1-4, n = 10) were given medicine by gavage daily according to the combination ratio of Example 1 at 1-fold, 2-fold, and 4-fold doses respectively; (4) The mice in the Example 2 group (S2, n = 10) were given medicine by gavage daily according to the combination ratio of Example 2; (5) The mice in the Comparative Example 1 group (S3, n = 10) were given medicine by gavage daily according to the combination ratio of Comparative Example 1; (6) The mice in the Comparative Example 2 group (S4, n = 10) were given medicine by gavage daily according to the combination ratio of Comparative Example 2; (7) The mice in the Comparative Example 3 group (S5, n = 10) were given medicine by gavage daily according to the combination ratio of Comparative Example 3. Each group was given medicine for 7 days. 40 minutes after the last dose, the mice were individually placed in a 125 ml wide-mouth bottle containing about 5 g of soda lime, sealed with a rubber stopper, and the death time of the animals was closely observed and recorded. After taking out the mice, serum was collected to detect relevant biochemical indexes.

[0058] (2) Results: From Figure 1 It can be seen that different embodiments of the medicine-food homologous composition can significantly increase the survival time of mice under closed hypoxia. Among them, Example 2 can increase the closed hypoxia time of mice by 31.39%, and the increase amplitude is the most significant (see Figure 1 B in). From Figure 1 It can be seen that compared with Example 2, after the proportion of ginseng powder is increased, the anti-hypoxia effect is reduced, indicating that the effect is the best when the addition amount of ginseng powder is 3 parts, and increasing the addition amount of ginseng powder is not beneficial to the anti-hypoxia effect. Moreover, by comparing S1-2 and Example 2, it can be seen that when ginseng powder is added, the anti-hypoxia effect of the composition is significantly better than that of the composition added with ginseng extract.

[0059] From Figure 2 It can be seen that after administration of the medicine-food homologous composition, it can reduce the levels of serum LDH, LACT, GLUT and UA in mice, indicating that this composition can improve the anti-hypoxia ability of mice by adjusting the metabolic disorder after closed hypoxia in mice. Among them, the anti-hypoxia effect of Example 2 is better than that of Examples 3-5.

[0060] Experimental Example 2 Verification of the anti-hypoxic fatigue effect of the medicine-food homologous composition on hypoxic swimming fatigue mice (1) Method: 70 male KM mice (weighing 18-22 g) were caged and raised in a clean, constant temperature (23±2°C) and constant humidity (55±10%) animal house, and the daily 12 h / 12 h day-night light cycle was adjusted artificially, with free drinking water and food. After 3 days of adaptive feeding, the 70 mice were randomly grouped: (1) Normal control group (Con, n = 10) mice were given saline by gavage daily; (2) Model group (Mod, n = 10) mice were given saline by gavage daily; (3) Positive control group (Ca, n = 10) mice were given 10 mg / kg caffeine solution by gavage daily; (4) Example 1 group (S1-1, S1-2, S1-4, n = 10) mice were given gavage daily according to the combination ratio of Example 1; (5) Example 2 group (S2, n = 10) mice were given gavage daily according to the combination ratio of Example 2. Except for the Con group, the mice in the other groups were placed in a low-pressure and low-oxygen chamber simulating an altitude of 6000 m for 7 days, and drugs were administered to each group at the same time. 40 minutes after the last administration, the mice were lowered to an altitude of 3500 m, and lead sheets accounting for 5% of the mouse body weight were tied to the backs of the mice with string, and the mice were put into water at 23°C for swimming. After timing for 10 minutes, the mice were taken out, serum was collected, and relevant biochemical indexes were detected.

[0061] (2) Results: From Figure 3It can be seen that after administration of the medicine-food homology composition, the levels of CK, BUN, LDH, LACT, and UA in the serum of mice can be significantly reduced, and the level of GLUT in the serum of mice can be significantly increased, indicating that this composition can reduce the accumulation of lactic acid during fatigue in mice, reduce glucose consumption during hypoxic exercise, thereby improving hypoxic exercise endurance, adjusting the metabolic disorder during hypoxic swimming in mice, and enhancing the anti-hypoxia and anti-fatigue abilities of mice. Among them, the improvement effect of Example 2 is the most significant.

[0062] Experimental Example 3 Verification of the Efficacy of the Medicine-Food Homology Composition on Mice with Hypoxic Pulmonary Vascular Injury (1)Method: Fifty male KM mice (weighing 18 - 22 g) were separately housed in a clean constant temperature (23 ± 2 °C) and constant humidity (55 ± 10%) animal room, and the daily 12 h / 12 h day-night light cycle was artificially adjusted, with free access to water and food. After 3 days of adaptive feeding, the 50 mice were randomly grouped: (1) Normal control group (Con, n = 10), the mice were given normal saline by gavage daily; (2) Model group (Mod, n = 10), the mice were given normal saline by gavage daily; (3) Positive control group (Dex, n = 10), the mice were given 1.04 mg / kg dexamethasone solution by gavage daily; (4) Example 1 group (S1-2, n = 10), the mice were given gavage according to the combination ratio of Example 1 daily; (5) Example 2 group (S2, n = 10), the mice were given gavage according to the combination ratio of Example 2 daily. After continuous administration for 10 days, lipopolysaccharide (0.625 mg / kg) was instilled into the nasal cavity on the 7th day. Except for the Con group, the other groups were placed in a low-pressure and low-oxygen chamber simulating an altitude of 6000 m for 3 days. After the mice were taken out, ultrasonic detection was performed, and serum and bronchoalveolar lavage fluid (BALF) were collected to detect blood routine and related biochemical indexes.

[0063] (2)Results: From Figure 4 It can be seen that different examples of this medicine-food homology composition can significantly inhibit the inflammatory response of mice, reduce the number of four types of cells, namely WBC, NEUT, LYMPH, and BASO, in the serum, and reduce the number of four types of cells, namely WBC, NEUT, LYMPH, and MONO, in the BALF; at the same time, reduce the levels of TNF-α, IL-6, IL-1β, and IFN-γ in the serum, reduce the levels of LDH and ALP in the serum and BALF, and improve the pulmonary vascular permeability. Among them, the improvement effect of Example 2 is the most significant.

[0064] From Figure 5It can be seen that after administration of the medicine-food homologous composition, it can significantly increase the cardiac output, stroke volume, and ejection fraction of the heart in mice, reduce the end-systolic volume, and reduce the percentage of the wall area in the total vascular area and the percentage of the arteriolar wall thickness in the vessel diameter, that is, reduce the vascular wall thickness, indicating that this composition can play an anti-hypoxic pulmonary vascular injury role by adjusting the hemodynamics of mice and inhibiting vascular remodeling.

Claims

1. A medicine-food composition, characterized in that: Calculated by weight, the invention comprises: 13.5-54 parts of genkwa root extract, 10-60 parts of seabuckthorn extract, and 3-30 parts of ginseng extract or ginseng powder.

2. The medicine-food composition according to claim 1, characterized in that: Calculated by weight, it includes: 27 parts of genkwa root extract, 30 parts of seabuckthorn extract, and 3 parts of ginseng extract or ginseng powder.

3. The medicine-food composition according to claim 1, characterized in that: Calculated by weight, the composition comprises: 27 parts of genkwa root extract, 30 parts of seabuckthorn extract and 3 parts of ginseng powder.

4. The method for preparing the edible-medicinal composition according to any one of claims 1 to 3, characterized in that: The extract of genkwa root, the extract of sea buckthorn and the extract of ginseng or ginseng powder are mixed to obtain a medicine-food composite.

5. The method for preparing the medicine-food composition according to claim 4, characterized in that: The preparation method of the genus dahurica extract is as follows: taking the genus dahurica, adding water, boiling it over high heat after it is softened, then decocting it over low heat, reserving the decoction after the decoction is completed, adding water to the obtained medicinal residue and decocting it again, repeating the decoction several times, combining the decoction, filtering it, and concentrating it under reduced pressure.

6. The method for preparing the edible-medicinal composition according to claim 4, characterized in that: The preparation method of the seabuckthorn extract comprises the following steps: taking seabuckthorn, adding water, and boiling over high heat after softening the seabuckthorn, then decocting over low heat, reserving the decoction after the decoction is completed, adding water to the obtained medicinal residue and decocting again, repeating the decoction several times, combining the decoction, filtering, and concentrating under reduced pressure.

7. The method for preparing the medicine-food composition according to claim 4, characterized in that: The preparation method of the ginseng extract comprises the following steps: taking ginseng, adding water, boiling over high heat after softening, decocting over low heat, reserving the decoction after the decoction is completed, adding water to the obtained medicinal residue and decocting again, repeating the decoction several times, combining the decoction, filtering, and concentrating under reduced pressure.

8. The method for preparing the medicine-food composition according to claim 4, characterized in that: The preparation method of the ginseng powder comprises the following steps: drying the ginseng slices, crushing them and sieving them.

9. Use of the medicine-food composition according to any one of claims 1 to 3 in the preparation of functional foods, health products or medicines for resisting hypoxia fatigue.

10. Use of the medicine-food composition according to any one of claims 1 to 3 in preparing functional foods, health products or medicines for treating hypoxic pulmonary vascular damage.

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