Multi-effect mild instant-washing instant-care infant bathing mousse and preparation method thereof

Abstract

The invention provides a multi-effect mild instant-washing instant-care infant bathing mousse and a preparation method thereof. The infant bathing mousse is prepared from the following raw materials in percentage by mass: 18.9 to 23.1 weight percent of a surfactant, 4.518 to 5.522 weight percent of a humectant, 0.9 to 1.1 weight percent of an antioxidant, 0.36 to 0.44 weight percent of a pH (Potential of Hydrogen) regulator, 0.009 to 0.011 weight percent of a chelating agent, 0.0009 to 0.0011 weight percent of an emollient and the balance of a solvent, wherein the surfactant is prepared from water, an anionic surfactant, hexanediol, tetrasodium glutamate diacetate, a zwitterionic surfactant, sodium chloride and a nonionic surfactant according to the mass ratio of (12.564 to 15.356) to (3.213 to 3.927) to (0.18 to 0.22) to 0.018 to 0.022 to (1.08 to 1.32) to (0.36 to 0.44) to (1.485 to 1.815), and the nonionic surfactant is decyl glucoside. The nonionic surfactant is composed of sodium lauroyl glutamate and sodium cocoyl apple amino acid, the anionic surfactant is composed of sodium lauroyl glutamate and sodium cocoyl apple amino acid according to the mass ratio of 2.295-2.805 wt.%: 0.918-1.122 wt.%, and the nonionic surfactant and the anionic surfactant have a synergistic effect, so that the infant bathing mousse can keep a uniform liquid phase in a temperature range of-10 DEG C to 60 DEG C and is free of crystallization or layering phenomena.

Description

Technical Field

[0001] The present invention relates to the daily chemical field, and specifically, to a multi-effect mild instant wash-and-care baby bath mousse and a preparation method thereof. Background Art

[0002] The skin water content of infants and young children is relatively high, but the stratum corneum is relatively thin, and the transdermal water loss rate is high; the skin is relatively delicate, the hair is not much and very soft; the sweat gland density is greater than that of adults, the function is weak, the sweating temperature is slightly higher than that of adults, and the total amount of lipids secreted by the sebaceous glands is less; the skin barrier is weak and is more sensitive to external stimuli. Therefore, for baby care products, the raw materials need to be strictly screened to reduce the introduction of impurities. On the basis of meeting the core properties (cleaning and foam), the types and dosages of raw materials used should be reduced as much as possible, and raw materials such as fragrances, preservatives, and even colorants that are likely to cause adverse reactions to the delicate skin of infants and young children should be used with caution.

[0003] Currently, baby products on the market still pay too much attention to market publicity and ignore the design concept of the products themselves. There are generally problems such as average cleaning degree, sticky skin after washing, insufficient foam, and jelly-like state at low temperature. For example, only amino acid surfactants are used for cleaning. If the dosage is too small, the cleaning power will be insufficient. If the dosage is too large, there will be a false slippery phenomenon during rinsing. Another example is to endow the product with active ingredients such as oats and chamomile or Chinese herbal medicine ingredients to adjust the skin feel or endow certain functions. However, because they may contain risk substances, such as methylisothiazolinone with cytotoxicity and DMDM hydantoin that may release formaldehyde, etc. Instead, it is easy to cause an increase in the irritation of the product, resulting in a stress response in the immature skin barrier of infants and young children. Summary of the Invention

[0004] Aiming at the defects in the prior art, the purpose of the present invention is to provide a multi-effect mild instant wash-and-care baby bath mousse and a preparation method thereof.

[0005] The technical solution of the present invention is as follows: In the first aspect of the present invention, a multi-effect mild instant wash-and-care baby bath mousse is provided. The baby bath mousse comprises raw materials in the following mass fractions: surfactant 18.9 - 23.1 wt.%, humectant 4.518 - 5.522 wt.%, antioxidant 0.9 - 1.1 wt.%, pH regulator 0.36 - 0.44 wt.%, chelating agent 0.009 - 0.011 wt.%, emollient 0.0009 - 0.0011 wt.%, and the balance is solvent; wherein, the surfactant comprises water, anionic surfactant, hexylene glycol, tetrasodium glutamate diacetate, zwitterionic surfactant, sodium chloride, and nonionic surfactant in a mass ratio of 12.564 - 15.356:3.213 - 3.927:0.18 - 0.22:0.018 - 0.022:1.08 - 1.32:0.36 - 0.44:1.485 - 1.815. The zwitterionic surfactant is sodium lauroamphoacetate; The nonionic surfactant is decyl glucoside, and the anionic surfactant is composed of sodium lauroyl glutamate and sodium cocoyl apple amino acid in a mass ratio of 2.295 - 2.805:0.918 - 1.122, so that the baby bath mousse remains a homogeneous liquid phase within the temperature range of -10°C to 60°C without crystallization or delamination.

[0006] Furthermore, the humectant comprises water, glycerol, 1,2 - hexanediol, and dipotassium glycyrrhizinate in a mass ratio of 0.0108 - 0.0132:3.5892 - 4.3868:0.9 - 1.1:0.018 - 0.022; the antioxidant comprises 1,3 - propanediol and octanoyl hydroxamic acid in a mass ratio of 0.828 - 1.012:0.072 - 0.088; the pH regulator is citric acid; the chelating agent is disodium EDTA; the emollient comprises castor oil, olive fruit oil, and ceramide NS / ceramide NG in a mass ratio of 0.0004275 - 0.0005225:0.0004275 - 0.0005225:0.000045 - 0.000055.

[0007] Further, the multi-effect mild wash-and-care baby bath mousse comprises raw materials with the following mass fractions: surfactant 21 wt.%, humectant 5.02 wt.%, antioxidant 1 wt.%, pH regulator 0.4 wt.%, chelating agent 0.01 wt.%, emollient 0.001 wt.%, and solvent 72.569 wt.%; wherein, the surfactant comprises water, sodium lauroyl glutamate, sodium cocoyl apple amino acid, hexylene glycol, tetrasodium glutamate diacetate, sodium lauroamphoacetate, sodium chloride, and decyl glucoside with a mass ratio of 13.94:2.55:1.04:0.2:0.02:1.2:0.4:1.65.

[0008] Further, the humectant comprises water, glycerol, 1,2-hexanediol, and dipotassium glycyrrhizinate with a mass ratio of 0.012:3.988:1:0.02; The antioxidant comprises 1,3-propanediol and octanoyl hydroxamic acid with a mass ratio of 0.92:0.08; The pH regulator is citric acid; The chelating agent is disodium EDTA; The emollient comprises castor oil, olive fruit oil, and ceramide NS / ceramide NG with a mass ratio of 0.000475:0.000475:0.00005 wt.%.

[0009] The second aspect of the present invention provides a preparation method of the multi-effect mild wash-and-care baby bath mousse, comprising the following steps: S1. Weigh the raw materials in the formula amount, and divide the raw materials into phase A raw materials and phase B raw materials; The phase A raw materials include: water, sodium lauroyl glutamate, glycerol, sodium cocoyl apple amino acid, hexylene glycol, tetrasodium glutamate diacetate, sodium lauroamphoacetate, sodium chloride, and decyl glucoside; The phase B raw materials include: 1,2-hexanediol, 1,3-propanediol, octanoyl hydroxamic acid, citric acid, dipotassium glycyrrhizinate, disodium EDTA, castor oil, olive fruit oil, and ceramide NS / ceramide NG; S2. Add the phase A raw materials into the main pot, turn on the stirring and heating until they are evenly mixed; S3. When the temperature of the main pot drops to 45-50 °C, add the phase B raw materials into the main pot and turn on the stirring until they are evenly mixed; S4. When the temperature of the main pot in step S3 drops to room temperature, obtain a semi-finished product, sample and test the semi-finished product, and filter it; S5. Transfer the semi-finished product in step S4; S6. Fill the semi-finished product in step S5 at room temperature to obtain the finished baby bath mousse.

[0010] Further, the heating temperature in step S2 is 75 - 80 °C.

[0011] Further, the mass ratio of the raw materials of phase A: water, sodium lauroyl glutamate, glycerol, sodium cocoyl apple amino acid, hexylene glycol, tetrasodium glutamate diacetate, sodium lauroamphoacetate, sodium chloride, decyl glucoside is 86.520000:2.550000:3.988000:1.020000:0.200000:0.020000:1.200000:0.400000:1.650000.

[0012] Further, the mass ratio of the raw materials of phase B: 1,2 - hexanediol, 1,3 - propanediol, octanoyl hydroxamic acid, citric acid, dipotassium glycyrrhizinate, disodium EDTA, castor oil, olive fruit oil, ceramide compound is 1.000000:0.920000:0.080000:0.400000:0.020000:0.010000:0.000475:0.000475:0.000050.

[0013] The pH range of the multi - effect mild wash - and - care baby bath mousse described above or the baby bath mousse prepared according to the preparation method of the multi - effect mild wash - and - care baby bath mousse is: 5.5 - 7.0.

[0014] Compared with the prior art, the present invention has the following beneficial effects: 1), The multi - effect mild wash - and - care baby bath mousse provided by the present invention synergistically uses decyl glucoside and sodium lauroyl glutamate to enhance the heat and cold resistance of the product, so that in high - temperature or low - temperature environments, the liquid state of the product remains stable and will not become thinner due to high temperature or jelly - like due to low temperature; sodium lauroyl glutamate, sodium cocoyl apple amino acid, decyl glucoside, and sodium lauroamphoacetate act synergistically to improve the foaming performance of the product and ensure that the generated foam has excellent stability.

[0015] 2) The multi - effect mild wash - and - care baby bath mousse provided by the present invention, by adding and adjusting the types and dosages of different surfactants, as well as the synergistic effect of components such as moisturizers and emollients, ensures that while gently cleaning, the skin of infants can also be effectively maintained. Moreover, the baby bath mousse provided by the present invention has good defoaming in water, high rinsability, weakens the significant irritation of surfactants to the skin, and is more suitable for use on infants. The formula of the present invention has a low cost, a simple preparation method, simple operation, and is suitable for large - scale production. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Other features, objects, and advantages of the present invention will become more apparent from the following detailed description of non-limiting embodiments read in conjunction with the accompanying drawings: Figure 1 Schematic diagram of the micelle particle size distribution in the baby bath mousse provided for the experimental examples of the present invention; Figure 2 Schematic diagram of the variation of foam height (Foam hight), liquid height, and total height in the baby bath mousse provided for the experimental examples of the present invention over time after foaming (0 s); Figure 3 Schematic diagram of the variation of the number of bubbles per square millimeter and the average bubble area in the baby bath mousse provided for the experimental examples of the present invention over time; Figure 4 Schematic diagram of the variation of the number and size of the foam in the baby bath mousse provided for the experimental examples of the present invention over time after foaming (0 s) ( Figure 4 Figure A in, intuitive schematic diagram of the variation of the number and size of the foam in the baby bath mousse over time after foaming (0 s), a) 150 s, b) 300 s, c) 450 s, d) 600 s, scale bar in the figure is 1 mm; Figure 4 Figure B in, schematic diagram of the variation of the number of foam and foam area in the baby bath mousse per square millimeter over time, a) 150 s, b) 300 s, c) 450 s, d) 600 s); Figure 5 Schematic diagram of the variation of the cell index of different treatment groups within 64 hours in the experimental examples of the present invention (NC: refers to the control group, without any intervention on the cells; treatment group: refers to adding baby bath mousse with concentrations of 0.062%, 0.031%, 0.015%, and 0.007% to the culture medium respectively); Figure 6 Schematic diagram of the effect of different concentrations of baby bath mousse on the expression of moisturizing genes in the experimental examples of the present invention ( Figure 6 Figure A in, NC: refers to the control group, without any intervention on the cells; 0.015%: refers to adding 0.015% of the baby bath mousse; Figure 6 Figure B in, NC: refers to the control group, without any intervention on the cells; 0.007%: refers to adding 0.007% of the baby bath mousse); Figure 7 Schematic diagram of the RNA-seq results in the experimental examples of the present invention ( Figure 7 Figure A in, schematic diagram of the RNAseq differential gene heat map results; Figure 7Figure B in [document], schematic diagram of KEGG enrichment analysis results; Figure 7 Figure C in [document], schematic diagram of GO enrichment analysis (up-regulated) results; Figure 7 Figure D in [document], schematic diagram of GO enrichment analysis (down-regulated) results). Detailed implementation manners

[0017] The present invention will be described in detail below in conjunction with specific embodiments.

[0018] The following embodiments will help those skilled in the art to further understand the present invention, but do not limit the present invention in any form. It should be noted that for those of ordinary skill in the art, without departing from the concept of the present invention, several changes and improvements can still be made, and these all belong to the protection scope of the present invention.

[0019] First Embodiment This embodiment provides a multi-effect mild instant wash-and-care baby bath mousse and its preparation method.

[0020] The multi-effect mild instant wash-and-care baby bath mousse comprises the following raw materials by mass fraction: surfactant 21 wt.%, humectant 5.020 wt.%, antioxidant 1 wt.%, pH regulator 0.4 wt.%, chelating agent 0.01 wt.%, emollient 0.001 wt.%, and the balance is solvent; the solvent in this embodiment is water.

[0021] Among them, the surfactant comprises water, sodium lauroyl glutamate, sodium cocoyl apple amino acid, hexylene glycol, tetrasodium glutamate diacetate, sodium lauroamphoacetate, sodium chloride, and decyl glucoside with a mass ratio of 13.94:2.55:1.04:0.2:0.02:1.2:0.4:1.65.

[0022] In this embodiment, decyl glucoside, sodium lauroyl glutamate, and sodium cocoyl apple amino acid act synergistically to keep the baby bath mousse in this embodiment in a homogeneous liquid phase within the temperature range of -10°C to 60°C, without crystallization or stratification.

[0023] The humectant comprises water, glycerol, 1,2-hexanediol, and dipotassium glycyrrhizinate with a mass ratio of 0.012:3.988:1:0.02.

[0024] The antioxidant comprises 1,3-propanediol and octanoyl hydroxamic acid with a mass ratio of 0.92:0.08.

[0025] The pH regulator is citric acid.

[0026] The chelating agent is disodium EDTA.

[0027] The emollient includes castor oil, olive fruit oil, ceramide NS / ceramide NG with a mass ratio of 0.000475:0.000475:0.00005.

[0028] The preparation method of the multi-effect mild wash-and-care baby bath mousse provided in this embodiment includes the following steps: S1. Weigh the raw materials in the formula amount, and the raw materials are divided into phase A raw materials and phase B raw materials; The phase A raw materials include water, sodium lauroyl glutamate, glycerin, sodium cocoyl apple amino acid, hexylene glycol, tetrasodium glutamate diacetate, sodium lauroamphoacetate, sodium chloride, and decyl glucoside with a mass ratio of 72.569:2.55:3.988:1.02:0.2:0.02:1.2:0.4:1.65; The phase B raw materials include 1,2-hexanediol, 1,3-propanediol, octanohydroxamic acid, citric acid, dipotassium glycyrrhizinate, disodium EDTA, castor oil, olive fruit oil, ceramide NS / ceramide NG with a mass ratio of 1:0.92:0.08:0.4:0.02:0.010:0.000475:0.000475:0.00005; S2. Add the phase A raw materials to the main pot, start stirring and heating, and the heating temperature is 75 - 80 °C until evenly mixed; S3. When the temperature of the main pot drops to 45 - 50 °C, add the phase B raw materials to the main pot and start stirring until evenly mixed; S4. When the temperature of the main pot in step S3 drops to room temperature, obtain the semi-finished product, sample and test the semi-finished product, and then filter it through a 200-mesh filter screen. (Take the semi-finished product to test whether its pH value is within the range of 5.5 - 7.0, and its appearance is a colorless to light yellow liquid); S5. Transfer the semi-finished product in step S4 to a storage container; S6. Fill the semi-finished product transferred to the storage container in step S5 at room temperature to obtain the finished product of the multi-effect mild wash-and-care baby bath mousse.

[0029] The pH range of the multi-effect mild wash-and-care baby bath mousse provided in this embodiment is: 5.5 - 7.0.

[0030] Second Embodiment The multi-effect mild wash-and-care baby bath mousse provided in this embodiment includes the following raw materials by mass fraction: surfactant 18.9 wt.%, humectant 5.02 wt.%, antioxidant 0.9 wt.%, pH regulator 0.36 wt.%, chelating agent 0.009 wt.%, emollient 0.0009 wt.%, and the balance is solvent; the solvent in this embodiment is water.

[0031] Among them, the surfactant includes water, sodium lauroyl glutamate, sodium cocoyl apple amino acid, hexylene glycol, tetrasodium glutamate diacetate, sodium lauroamphoacetate, sodium chloride, and decyl glucoside with a mass ratio of 12.564:2.295:0.918:0.18:0.018:1.08:0.36%:1.485.

[0032] In this embodiment, decyl glucoside, sodium lauroyl glutamate, and sodium cocoyl apple amino acid act synergistically to keep the baby bath mousse of this embodiment in a homogeneous liquid phase within the temperature range of -10°C to 60°C, without crystallization or stratification.

[0033] The moisturizer includes water, glycerol, 1,2 - hexanediol, and dipotassium glycyrrhizinate with a mass ratio of 0.0108:3.5892:0.9:0.018.

[0034] The antioxidant includes 1,3 - propanediol and octanoyl hydroxamic acid with a mass ratio of 0.828:0.072.

[0035] The pH regulator is citric acid.

[0036] The chelating agent is disodium EDTA.

[0037] The emollient includes castor oil, olive fruit oil, ceramide NS / ceramide NG with a mass ratio of 0.0004275, 0.0004275, 0.000045.

[0038] The multi - effect mild wash - and - care baby bath mousse provided in this embodiment has a preparation method including the following steps: S1. Weigh the raw materials in the formula amount, and the raw materials are divided into phase A raw materials and phase B raw materials; The phase A raw materials include water, sodium lauroyl glutamate, glycerol, sodium cocoyl apple amino acid, hexylene glycol, tetrasodium glutamate diacetate, sodium lauroamphoacetate, sodium chloride, and decyl glucoside with a mass ratio of 95.1951:1.000000:12.564:3.5892:0.918:0.18:0.018:1.08:0.36%:1.485; The phase B raw materials include 1,2 - hexanediol, 1,3 - propanediol, octanoyl hydroxamic acid, citric acid, dipotassium glycyrrhizinate, disodium EDTA, castor oil, olive fruit oil, ceramide NS / ceramide NG with a mass ratio of 0.9:0.828:0.072:0.36:0.018:0.009:0.0004275, 0.0004275, 0.000045; S2. Add the phase A raw materials to the main pot, turn on the stirring and heat, and the heating temperature is 75 - 80°C until it is evenly mixed; S3. When the temperature of the main pot drops to 45 - 50 °C, add the raw materials of Phase B to the main pot and start stirring until evenly mixed. S4. When the temperature of the main pot in Step S3 drops to room temperature, obtain the semi-finished product. Take a sample of the semi-finished product for testing, and then filter it through a 200-mesh filter screen. (Take the semi-finished product to test whether its pH value is within the range of 5.5 - 7.0, and its appearance is a colorless to light yellow liquid); S5. Transfer the semi-finished product in Step S4 to a storage container. S6. Fill the semi-finished product transferred to the storage container in Step S5 at room temperature to obtain the finished product of the multi-effect mild and wash-and-care baby bath mousse.

[0039] The multi-effect mild and wash-and-care baby bath mousse provided in this embodiment has a pH range of: 5.5 - 7.0.

[0040] Third Embodiment This embodiment provides a multi-effect mild and wash-and-care baby bath mousse and its preparation method.

[0041] The multi-effect mild and wash-and-care baby bath mousse includes raw materials with the following mass fractions: surfactant 23.1 wt.%, humectant 5.522 wt.%, antioxidant 1.1 wt.%, pH regulator 0.44 wt.%, chelating agent 0.011 wt.%, emollient 0.0011 wt.%, and the balance is solvent; Among them, the surfactant includes water, sodium lauroyl glutamate, sodium cocoyl apple amino acid, hexylene glycol, tetrasodium glutamate diacetate, sodium lauroamphoacetate, sodium chloride, and decyl glucoside with a mass ratio of 15.356:2.805:1.122:0.22:0.022:1.32:0.44:1.815.

[0042] In this embodiment, decyl glucoside, sodium lauroyl glutamate, and sodium cocoyl apple amino acid act synergistically to keep the baby bath mousse in this embodiment in a homogeneous liquid phase within the temperature range of -10 °C to 60 °C, without crystallization or stratification.

[0043] The humectant includes water, glycerol, 1,2-hexanediol, and dipotassium glycyrrhizinate with a mass ratio of 0.0132:4.3868:1.1:0.022.

[0044] The antioxidant includes 1,3-propanediol and octanoyl hydroxamic acid with a mass ratio of 1.012:0.088.

[0045] The pH regulator is citric acid.

[0046] The chelating agent is disodium EDTA.

[0047] The emollient includes castor oil, olive fruit oil, and ceramide NS / ceramide NG in a mass ratio of 0.0005225:0.0005225:0.000055.

[0048] The multi-effect mild wash-off and leave-on baby bath mousse provided in this embodiment has a preparation method including the following steps: S1. Weigh the raw materials in the formula amount, and the raw materials are divided into phase A raw materials and phase B raw materials; The phase A raw materials include water, sodium lauroyl glutamate, glycerin, sodium cocoyl apple amino acid, hexylene glycol, tetrasodium glutamate diacetate, sodium lauroamphoacetate, sodium chloride, and decyl glucoside in a ratio of 77.8869:2.805:4.3868:1.122:0.22:0.022:1.32:0.44:1.815; The phase B raw materials include 1,2-hexanediol, 1,3-propanediol, octanoyl hydroxamic acid, citric acid, dipotassium glycyrrhizinate, disodium EDTA, castor oil, olive fruit oil, and ceramide NS / ceramide NG in a ratio of 1.1:1.012:0.088:0.44:0.022:0.011:0.0005225:0.0005225:0.000055; S2. Add the phase A raw materials to the main pot, turn on the stirring and heat, and the heating temperature is 75 - 80 °C until evenly mixed; S3. When the temperature of the main pot drops to 45 - 50 °C, add the phase B raw materials to the main pot and turn on the stirring until evenly mixed; S4. When the temperature of the main pot in step S3 drops to room temperature, obtain the semi-finished product. Take a sample of the semi-finished product for testing, and then filter it through a 200-mesh filter screen. (Take the semi-finished product to test whether its pH value is within the range of 5.5 - 7.0, and its appearance is a colorless to light yellow liquid); S5. Transfer the semi-finished product in step S4 to a storage container; S6. Fill the semi-finished product transferred to the storage container in step S5 at room temperature to obtain the finished product of the multi-effect mild wash-off and leave-on baby bath mousse.

[0049] The multi-effect mild wash-off and leave-on baby bath mousse provided in this embodiment has a pH range of 5.5 - 7.0.

[0050] Experimental Example This experimental example selects the first embodiment as the experimental sample to prove through the following experiments that the multi-effect mild wash-off and leave-on baby bath mousse provided by the present invention has the characteristics of rapid foaming and strong foam stability; there is no layering or turbidity phenomenon under high-temperature and low-temperature environments, and its liquid state is less affected by temperature.

[0051] 1. Experimental part 1.1 Reagents and Instruments Sodium Lauroyl Glutamate (EversoftTM ULS-30SN, active matter content 20-31%, Nanjing Huashi New Materials Co., Ltd.); Decyl Glucoside (Plantacare® 2000 UP, active matter content 51-55%, BASF (China) Co., Ltd.); Sodium Lauroamphoacetate (lauroyl amphocarboxylate imidazoline, active matter content 28-33%, Transfar Smart Link Co., Ltd.); Sodium Cocoyl Apple Amino Acid (PROTEOL APL EF, active matter content 22-30%, SEPPIC S.A.); Glycerol (MASCEROL Glycerine 99.7% USP, P. T. Musim Mas); Octanohydroxamic Acid, 1,3-Propanediol (Beijing Sump Biochem Co., Ltd.); 1,2-Hexanediol (Shinsung Materials Co., LTD); Citric Acid (Weifang Yingxuan Industry Co., Ltd.); Castor (RICINUS COMMUNIS) Seed Oil, Olive (OLEA EUROPAEA) Fruit Oil, Ceramide NS / Ceramide NG (Evonik Operations GmbH).

[0052] IKA EUROSTAR 40 Digital Display Stirrer, IKA Company, Germany; FiveEasy Plus pH Meter FE28-Standard, Mettler Toledo International Co., Ltd.; Dynamic Foam Analyzer DFA100, Krüss Company, Germany; omni Nano Particle Size Zeta Potential Analyzer, Brookhaven Company, USA.

[0053] 1.2 Testing of Physical and Chemical Indexes The heat resistance stability, cold resistance stability and pH were determined according to GB / T 29679-2013 "Shampoo, Hair Cream". The foaming performance and foam stability of the product formula were characterized by using the Dynamic Foam Analyzer DFA100 of Krüss Company, Germany.

[0054] 5 mL of the experimental sample (provided by the first embodiment) was diluted 1:10 with deionized water to 50 mL, and foamed by the method of shear foaming. The shear rate was set at 3500 rpm, the shear time was 3 s, the change of foam with time was recorded by images, the analysis time was 700 s, and the test temperature was 25 °C. The micelle particle size and zeta potential of the surfactant in the experimental sample were characterized by an omni Nano Particle Size Zeta Potential Analyzer at 25 °C.

[0055] 1.3 Cell Culture and Activity Testing The human immortalized keratinocyte HaCaT was cultured in DMEM medium supplemented with 10% FBS and 1% penicillin-streptomycin solution (100 μg / mL) in a cell incubator (37 °C, 5% CO2) and passaged after 24 hours. Log-phase HaCaT cells were seeded into the E-Plate assay plate of the Real-Time Cell Analyzer (RTCA) SP instrument (1×10 4 cells / well). After 12 hours of cell attachment, the medium was aspirated, and different concentrations of the medium containing the experimental samples were added as the treatment groups (the concentrations of the multi-effect mild wash-and-go baby bath mousse in the medium of the experimental samples were 0.062%, 0.031%, 0.015%, and 0.007% respectively). The cell index was monitored every 10 minutes within 64 hours.

[0056] 1.4 Gene expression assay (qPCR) HaCaT cells were seeded into 6-well plates (3×10 5 cells / well). After 24 hours of culture, the medium was aspirated, the cells were washed with PBS solution, and then the PBS was aspirated. Different concentrations of the medium containing the experimental samples were added. After 24 hours of culture, the cells were collected, and total cellular RNA was extracted using the TRIzol method. The purity and concentration of the RNA were detected using NanoDrop. cDNA was synthesized using a reverse transcription kit, and gene phenotype detection was performed on a fluorescence quantitative PCR instrument using the relevant primers and qPCR premix. The details of the gene primers are shown in Table 1.

[0057] 1.5 RNA sequencing and analysis After the total cellular RNA was extracted by the TRIzol method, Shanghai OE Biotech Co., Ltd. performed library construction and RNA sequencing. The DESeq2 software was used to analyze the gene expression differences between the treatment groups (added with different concentrations of the multi-effect mild wash-and-go baby bath mousse) and the control group (without any intervention). The false discovery rate (FDR) less than 0.05 and the absolute fold change ≥2 were used as the parameters to identify differentially expressed genes. Molecular function, cellular component, and biological process are the three components of Gene Ontology (GO), which is a globally standardized gene classification system. The GO terms with a p-value less than 0.05 were defined as the significantly enriched GO terms in the DEGs (differentially expressed genes). According to the pathway enrichment analysis of the Kyoto Encyclopedia of Genes and Genomes (KEGG), compared with the whole genome background, the DEGs were significantly enriched in metabolic or signal transduction pathways.

[0058] 2. Results and discussion 2.1 Physicochemical properties 2.1.1 Liquid stability of the multi-effect mild wash-and-go baby bath mousse The pH of the multi-functional mild and instant-use baby bath mousse measured by the dilution method is maintained within the range of 5.5 - 7, showing a slightly acidic overall. The prepared multi-functional mild and instant-use baby bath mousse was placed in a constant temperature incubator at (40 ± 1) °C and (-8 ± 2) °C respectively for 24 h. After taking it out of the incubator and waiting for it to return to room temperature, the morphological changes of the multi-functional mild and instant-use baby bath mousse were observed. The multi-functional mild and instant-use baby bath mousse did not show phenomena such as stratification and turbidity under the two temperature conditions, indicating that the multi-functional mild and instant-use baby bath mousse provided by the present invention passed the heat and cold resistance stability test.

[0059] The above results show that the multi-functional mild and instant-use baby bath mousse provided by the present invention is mild (slightly acidic) in use and has good liquid stability.

[0060] Alkyl polyglycosides, such as decyl glucoside, can tolerate a wide temperature range. At higher temperatures (40 - 60 °C), they can still maintain excellent surface activity performance and detergency, and can also remain stable at low temperatures (-10 °C - -5 °C), not easily crystallizing or stratifying, and their solubility and surface activity performance are basically not affected. Amino acid surfactants, such as sodium lauroyl glutamate, are relatively sensitive to temperature changes when used alone. For example, crystallization or precipitation may occur at low temperatures. However, when using alkyl polyglycosides in synergy with amino acids, the heat and cold resistance of the baby bath mousse can be improved, so that the liquid state of the baby bath mousse can still remain stable in high or low temperature environments, and will not become thin due to high temperature or jelly-like due to low temperature.

[0061] 2.1.2 Stability and foaming properties of the multi-functional mild and instant-use baby bath mousse The stability of the multi-functional mild and instant-use baby bath mousse was preliminarily characterized by dynamic light scattering method. The average particle size of the surfactant micelles in the multi-functional mild and instant-use baby bath mousse measured by dynamic light scattering method is 5.57 ± 0.04 nm, indicating that the size of these micelles is very small and uniform. This small size helps the micelles to disperse and stabilize better in the bath mousse, thus improving the use effect and stability of the product. In addition, the surfactant micelles in the multi-functional mild and instant-use baby bath mousse have a narrow polydispersity index of only 0.13, indicating that the size distribution of these micelles is very concentrated. A lower polydispersity index means that the size difference of the micelles is small, further proving the uniformity and stability of the micelles. As Figure 1As shown, the micelle distribution is uniform in size and concentrated. The two data of the average particle size and the polydispersity index together indicate that the surfactant micelles in the multi-effect mild and wash-and-care baby bath mousse have excellent stability and uniformity. This property helps to ensure consistent performance during product use, while reducing potential irritation to the baby's skin and providing a more gentle cleansing and care experience.

[0062] The measured micelle zeta potential of the multi-effect mild and wash-and-care baby bath mousse is approximately -17.17 mV, indicating that the micelle surfactant is negatively charged and there is a certain electrostatic repulsion between the micelles. The system has moderate stability and meets the requirements for mildness and safety of baby bath mousses.

[0063] The foaming performance of the multi-effect mild and wash-and-care baby bath mousse was preliminarily characterized by a dynamic foam analyzer. 5 mL of the multi-effect mild and wash-and-care baby bath mousse was diluted 10 times with deionized water and added to the dynamic foam analyzer. Foaming was carried out using the shear method. At a shear rate of 3500 rpm and a shear time of 3 s, the results are as Figure 2 shown. The maximum foam height is reached after approximately 100 s, and the maximum foam height is approximately 115 mm. The foam height remains for approximately 350 s and then gradually decreases. It can be seen that the multi-effect mild and wash-and-care baby bath mousse provided by the present invention has the characteristics of fast foaming and stable foam.

[0064] Sodium lauroyl glutamate and sodium cocoyl apple amino acid both have the characteristics of delicate foam texture, soft touch, and good foam stability; decyl glucoside and sodium lauroamphoacetate have excellent foaming ability and rich and delicate foam; the synergistic effect of the four surfactants makes the multi-effect mild and wash-and-care baby bath mousse provided by the present invention have excellent foaming performance and stable foam. In addition, the four surfactants all have the characteristics of gentle cleansing without irritation and are suitable for sensitive skin people and babies.

[0065] By analyzing the foam images, the change of the number of foams with time can be obtained, as Figure 3As shown, after foaming for 150 s, the number of foam cells per square millimeter reaches 205, and the average foam area is approximately 4800 µm². The foam is small and dense. As time extends, the foam area gradually becomes larger, and the number of foam cells per square millimeter decreases. The half-life of the number of bubbles is approximately 300 s. At 700 s, the number of foam cells per square millimeter decreases to 15, and the average foam area increases to 64000 µm². This indicates that the multi-effect mild wash-and-care baby bath mousse product provided by the present invention has excellent foaming performance and can quickly form a large number of small and dense foams. Such foam characteristics are usually related to good cleaning effects and user experiences. Especially for baby bath products, small and dense foams are more likely to be evenly distributed on the skin surface, providing a mild cleaning effect.

[0066] The changes in the number of foam cells per square millimeter and the foam area over time are as Figure 4 shown. At 150 s, the foam is of uniform size, small and dense. As time extends, the foam becomes larger and will gradually break ( Figure 4 A). The foam gradually evolves from the initial small and dense state to a large and sparse state, which is due to processes such as foam coalescence and rupture between the foams. Small and dense foams can better cover the skin surface, enhancing the cleaning effect while reducing friction and irritation to the delicate skin of infants. As time changes, the number of large bubbles increases ( Figure 4 B), further confirming the phenomenon of foam coalescence and rupture. The gradual rupture and coalescence of the foam indicate that the product is easy to clean after use and will not leave too much foam residue on the skin, meeting the requirements of mildness and ease of use for baby bath products.

[0067] 2.2 Biological properties 2.2.1 Effects of the multi-effect mild wash-and-care baby bath mousse on cell proliferation The RTCA cell proliferation curve shows the effects of the multi-effect mild wash-and-care baby bath mousse provided by the present invention on HaCaT cells. As Figure 5 shown, during the experiment, there were no significant differences in cell proliferation between the four treatment groups (the concentrations of the multi-effect mild wash-and-care baby bath mousse were 0.062%, 0.031%, 0.015%, and 0.007% respectively) and the control group (NC). This result indicates that the multi-effect mild wash-and-care baby bath mousse provided by the present invention has no cytotoxicity to HaCaT and has good safety.

[0068] 2.2.2 Effects of the multi-effect mild wash-and-care baby bath mousse on the expression of moisturizing genes in HaCaT cells The qPCR results are as Figure 6 shown. Compared with the control group, in the two treatment groups ( Figure 6The concentration of the multi-effect mild wash-and-care baby bath mousse in A is 0.015%; Figure 6 The mRNA expression levels of KRT5, E-cadherin, Occludin, and Claudin-1 genes in B (the concentration of the multi-effect mild wash-and-care baby bath mousse is 0.007%) all showed a certain upward trend. The expression level of the highest treatment group (the concentration of the multi-effect mild wash-and-care baby bath mousse is 0.015%) can reach 1.5 times that of the control group. Its upward trend reflects that the treatment group plays an important role in maintaining cell phenotype stability, enhancing cell adhesion ability, and improving barrier function. The results further prove that the baby bath mousse provided by the present invention can effectively promote skin moisturization and repair during use and has good skin care effects.

[0069] 2.2.3 RNAseq sequencing results of the multi-effect mild wash-and-care baby bath mousse The baby bath mousse provided by the present invention is mild and non-irritating to use, and at the same time can repair and protect the skin and form a protective barrier on the skin surface. To deeply study its mechanism of multi-effect mildness and wash-and-care, the RNAs of the treatment group (containing 0.015% of the multi-effect mild wash-and-care baby bath mousse) and the control group were subjected to high-throughput sequencing, and differentially expressed genes were screened out. The sequencing results were displayed by cluster analysis and heat map, as Figure 7 shown, 24 genes in HaCaT cells in the treatment group were significantly up-regulated and 27 genes were significantly down-regulated, involving various biological processes related to cell functions ( Figure 7 A).

[0070] GO functional enrichment analysis was performed on the RNA-seq results, and the significantly enriched biological processes and molecular function pathways were focused on. The results are as Figure 7 C, Figure 7 D shown. The differentially expressed genes in the treatment group showed significant changes in multiple functional pathways. The up-regulated and down-regulated pathways were respectively involved in cell protection, stress response, regulation of extracellular matrix metabolism, and signal transduction. The up-regulated genes were enriched in processes such as DNA protection, cell response to stimuli, positive regulation of collagen synthesis, and intercellular adhesion molecule activity ( Figure 7C). In addition, pathways related to cell proliferation and growth, such as positive regulation of cell growth, anchoring junction, and integrin binding, also showed significant enrichment, indicating that the baby bath mousse provided by the present invention can promote cell stability and regeneration ability by regulating the activity of extracellular matrix and adhesion molecules. The upregulation in the function of the extracellular region indicates that the baby bath mousse provided by the present invention can promote the stability and regeneration ability of cells by regulating the activity of extracellular matrix and adhesion molecules. The downregulated genes were significantly enriched in processes related to negative regulation and signal inhibition, such as p38 mitogen-activated protein kinase binding, negative regulation of epithelial cell migration, and fibroblast growth factor binding ( Figure 7 D). In addition, the downregulated genes are related to sphingosine biosynthesis and negative regulation of the Wnt signaling pathway, indicating that the baby bath mousse provided by the present invention can inhibit cell migration and inflammatory signals, while regulating the metabolic and signal networks to maintain cell homeostasis.

[0071] KEGG signaling pathway enrichment analysis was performed on the differentially expressed genes of RNA-seq, and the results are as Figure 7 shown in Figure B. The differential genes were significantly enriched in pathways such as gap junction, calcium signaling, MAPK signaling, cGMP-PKG signaling, and Wnt signaling. The enrichment of these pathways indicates that the baby bath mousse provided by the present invention can maintain cell homeostasis by regulating signal communication and metabolism. In particular, the Wnt signaling, which is closely related to cell proliferation, differentiation, and tissue repair.

[0072] The differential genes were also enriched in chromatin remodeling and metabolic pathways, such as pyruvate metabolism and cysteine and methionine metabolism. This indicates that the baby bath mousse provided by the present invention can act through the epigenetic mechanism of regulating gene expression, while regulating the levels of enzymes and substrates in key metabolic pathways to improve the metabolic state and adaptability of cells. Finally, the immune-related systemic lupus erythematosus pathway indicates that the baby bath mousse provided by the present invention can maintain skin immune balance by regulating immune signals.

[0073] In summary, the baby bath mousse provided by the present invention not only has the characteristics of being mild and non-irritating, but also can repair and protect the skin through multiple molecular mechanisms, enhance cell stability, regeneration ability, and barrier function, while regulating metabolism and immune balance, providing comprehensive care for the skin health of infants.

[0074] The specific embodiments of the present invention have been described above. It should be understood that the present invention is not limited to the above specific embodiments, and those skilled in the art can make various changes or modifications within the scope of the claims, which do not affect the essence of the present invention. Without conflict, the embodiments of the present application and the features in the embodiments can be combined arbitrarily.

Claims

1. A multi-effect, gentle, instant-wash and instant-care infant bath mousse, characterized in that: The infant bath mousse comprises the following raw materials in weight fractions: a surfactant 18.9-23.1 wt.%, a moisturizer 4.518-5.522 wt.%, an antioxidant 0.9-1.1 wt.%, a pH adjuster 0.36-0.44 wt.%, a chelating agent 0.009-0.011 wt.%, an emollient 0.0009-0.0011 wt.%, and the balance is a solvent; The surfactant includes water, anionic surfactant, hexylene glycol, tetrasodium glutamate diacetate, zwitterionic surfactant, sodium chloride, and nonionic surfactant in a mass ratio of 12.564-15.356:3.213-3.927:0.18-0.22:0.018:0.022:1.08-1.32:0.36-0.44:1.485-1.815; The zwitterionic surfactant is sodium lauroyl amphoteric acid; The nonionic surfactant is decyl glucoside, and the anionic surfactant is composed of sodium lauroyl glutamate and sodium cocoyl apple amino acid in a mass ratio of 2.295-2.805:0.918-1.122, so that the infant bath mousse maintains a uniform liquid phase within a temperature range of -10°C-60°C without crystallization or stratification.

2. The multi-effect mild instant-wash and instant-care infant bath mousse according to claim 1, characterized in that: The moisturizing agent includes water, glycerin, 1,2-hexanediol, and dipotassium glycyrrhizinate in a mass ratio of 0.0108-0.0132:3.5892-4.3868:0.9-1.1:0.018-0.022; The antioxidant includes 1,3-propylene glycol and caprylhydroxamic acid in a mass ratio of 0.828-1.012:0.072-0.088; The pH regulator is citric acid; The chelating agent is disodium EDTA; The emollient comprises castor seed oil, olive fruit oil, and ceramide NS / ceramide NG in a mass ratio of 0.0004275-0.0005225:0.0004275-0.0005225:0.000045-0.000055.

3. The multi-effect mild instant-wash and instant-care infant bath mousse according to claim 1, characterized in that: The infant bath mousse comprises the following raw materials by weight: 21 wt.% of surfactant, 5.02 wt.% of moisturizer, 1 wt.% of antioxidant, 0.4 wt.% of pH adjuster, 0.01 wt.% of chelating agent, 0.001 wt.% of emollient, and 72.569 wt.% of solvent; The surfactant includes water, sodium lauroyl glutamate, sodium cocoyl apple amino acid, hexylene glycol, tetrasodium glutamate diacetate, sodium lauroylamphoacetate, sodium chloride and decyl glucoside in a mass ratio of 13.94:2.55:1.04:0.2:0.02:1.2:0.4:1.

65.

4. The multi-effect mild instant-wash and instant-care infant bath mousse according to claim 1, characterized in that: The moisturizing agent includes water, glycerin, 1,2-hexanediol, and dipotassium glycyrrhizinate in a mass ratio of 0.012:3.988:1:0.02; The antioxidant includes 1,3-propylene glycol and caprylhydroxamic acid in a mass ratio of 0.92:0.08; The pH regulator is citric acid; The chelating agent is disodium EDTA; The emollient comprises castor seed oil, olive fruit oil, and ceramide NS / ceramide NG in a mass ratio of 0.000475:0.000475:0.00005 wt.%.

5. The method for preparing the multi-effect mild instant-wash and instant-protection infant bath mousse according to any one of claims 1 to 4, characterized in that: The preparation method comprises the following steps: S1. Weigh the raw materials in the formula, wherein the raw materials are divided into phase A raw materials and phase B raw materials; The raw materials of phase A include: water, sodium lauroyl glutamate, glycerin, sodium cocoyl apple amino acid, hexylene glycol, tetrasodium glutamate diacetate, sodium lauroyl amphoacetate, sodium chloride, and decyl glucoside; The phase B raw materials include: 1,2-hexanediol, 1,3-propylene glycol, caprylhydroxamic acid, citric acid, dipotassium glycyrrhizinate, disodium EDTA, castor seed oil, olive oil, ceramide NS / ceramide NG; S2. Add the ingredients of phase A into the main pot, start stirring and heat until mixed evenly; S3. When the temperature of the main pot drops to 45-50°C, add the ingredients of phase B into the main pot and start stirring until they are evenly mixed. S4, when the temperature of the main pot in step S3 is lowered to a room temperature, a semi-finished product is obtained, and the semi-finished product is sampled, tested, and filtered; S5, transferring the semi-finished product in step S4; S6. Fill the semi-finished product in step S5 at room temperature to obtain a finished product of infant bath mousse.

6. The method for preparing the multi-effect mild instant-wash and instant-protection infant bath mousse according to claim 5, characterized in that: The heating temperature in step S2 is 75-80°C.

7. The method for preparing the multi-effect mild instant-wash and instant-protection infant bath mousse according to claim 5, characterized in that: The mass ratio of the phase A raw materials: water, sodium lauroyl glutamate, glycerin, sodium cocoyl apple amino acids, hexylene glycol, tetrasodium glutamate diacetate, sodium lauroyl amphoacetate, sodium chloride, and decyl glucoside is 86.52:2.55:3.988:1.02:0.2:0.02:1.2:0.4:1.

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8. The method for preparing the multi-effect mild instant-wash and instant-protection infant bath mousse according to claim 5, characterized in that: The mass ratio of the phase B raw materials 1,2-hexanediol, 1,3-propylene glycol, caprylhydroxamic acid, citric acid, dipotassium glycyrrhizinate, disodium EDTA, castor seed oil, olive oil, and ceramide compound is 1:0.92:0.080000:0.4:0.02:0.01:0.000475:0.000475:0.00005.

9. The multi-effect mild instant-wash and instant-care infant bath mousse prepared according to any one of claims 1 to 4 or the preparation method of the multi-effect mild instant-wash and instant-care infant bath mousse according to any one of claims 5 to 8, characterized in that: The pH range of the infant bath mousse is 5.5-7.0.

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