Application of DDX51 gene in human melanoma and related product

CN120193023AActive Publication Date: 2025-06-24LANZHOU UNIV SECOND HOSPITAL +1
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Patent Information

Application Number
CN202510300751.3
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2025-03-14
Publication Date
2025-06-24
Estimated Expiration
2045-03-14

AI Technical Summary

Technical Problem

The prior art lacks effective treatments to deal with high malignancy and high mortality of human melanoma, especially in early diagnosis and treatment.

Method used

By designing the RNA interference target sequence of the DDX51 gene, RNA interference lentiviral vector is constructed to inhibit the expression of the DDX51 gene in human melanoma cells, thereby preparing drugs for the treatment of human melanoma.

Benefits of technology

Inhibit the proliferation, cloning, migration, metastasis and invasion of human melanoma cells, promote cell apoptosis, and effectively inhibit tumor growth.

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Abstract

The invention discloses application of a DDX51 gene in human melanoma and a related product. According to the invention, a DDX51 gene is taken as a template, an RNA interference target sequence is designed, and a target gene RNA interference lentiviral vector is constructed. Experiment for detecting expression of target genes in target cells by RT-PCR (reverse transcription-polymerase chain reaction), experiment for infecting target cells by lentivirus, experiment for detecting DDX51 gene knock-down efficiency of mRNA (messenger ribonucleic acid) level by qPCR (quantitative polymerase chain reaction), experiment for detecting influence of DDX51 gene knock-down on cell proliferation by Celigo, experiment for detecting influence of DDX51 gene knock-down on clone formation, and Invasion (Transwell with ECM) Experiments for detecting the influence of DDX51 gene knock-down on the cell invasion ability prove that the interference target gene DDX51 has the effects of inhibiting proliferation, clone formation, invasion and transfer of human melanoma cells A375.
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Citation Information

Patent Citations

  • Tumor metastasis inhibition method

    CN105886534A

  • New application of BTF3 interfering in inhibiting proliferation of melanoma cells

    CN107334777A

  • Application of human DDX10 gene and related product

    CN111518802A

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    CN112110999A

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    US20020165188A1