Synergistic suspending agent containing lufenuron

Abstract

The invention relates to the technical field of pesticides, and discloses a synergistic suspending agent containing lufenuron. 1000 parts by weight of the synergistic suspending agent comprises the following raw materials: 14-350 parts by weight of lufenuron, 10-250 parts by weight of indoxacarb, 10-200 parts by weight of a synergist, 50-400 parts by weight of an auxiliary agent and the balance of water, and the weight ratio of lufenuron to indoxacarb is 7: 5. The synergist is a mixture of a microbial metabolite and a polymer emulsifier, and the weight ratio of the microbial metabolite to the polymer emulsifier is (3: 1)-(1: 3). The synergistic suspending agent is used for preventing and treating spinach beet armyworms, the permeability and surface tension of the agent can be obviously improved, the disease and insect resistance of crops can be improved, the lasting period is long, and residues are small.

Description

Technical Field

[0001] The present invention belongs to the technical field of pesticides, and particularly relates to a synergistic suspending agent containing lufenuron and its application. Background Art

[0002] Lufenuron is a new generation of substituted urea insecticides. The agent kills pests by acting on insect larvae and preventing the molting process, and has a particularly good control effect on leaf-eating caterpillars such as fruit trees, and has a specific killing mechanism for thrips, rust mites, and whiteflies, and is suitable for controlling pests resistant to synthetic pyrethroids and organophosphorus pesticides.

[0003] Indoxacarb is an oxadiazine insecticide. It can effectively control a variety of pests on crops such as grains, cotton, fruits, and vegetables. It is suitable for controlling Spodoptera exigua, Plutella xylostella, Pieris rapae, Spodoptera litura, Mamestra brassicae, Helicoverpa armigera, Heliothis assulta, leaf roller moths, Cydia pomonella, Earias vittella, Leptinotarsa decemlineata, Cnaphalocrocis medinalis, and Chilo suppressalis on crops such as cabbage, cauliflower, Chinese kale, tomato, pepper, cucumber, zucchini, eggplant, lettuce, apple, pear, peach, apricot, cotton, potato, and grape.

[0004] Spinach Spodoptera exigua belongs to Lepidoptera and Noctuidae, and is a world-wide stubborn pest. In Daxian, Spodoptera exigua mainly harms crops such as onions, cowpeas, water spinach, radishes, Chinese cabbages, lettuce, cabbages, and kidney beans. The newly hatched larvae of Spodoptera exigua gather on the back of the leaves, spin silk to form a net, and feed on the mesophyll inside, leaving the epidermis, forming transparent small holes. After the 3rd instar, the leaves can be eaten into holes or notches. In severe cases, only the veins and petioles remain. As a result, the quality of spinach declines or loses its commercial value, and in severe cases, it causes a complete crop failure.

[0005] Spinach is a vegetable favored by consumers, and its market demand is increasing continuously. During the planting process, farmers often encounter pests such as spinach Spodoptera exigua, which greatly affects the planting benefits of farmers. With the increasing resistance of pests and the extensive use of traditional emulsifiable concentrate products, farmers use higher and higher concentrations of pesticides to achieve the control purpose. Through continuous innovative research, the present invention found that by mixing lufenuron and indoxacarb with completely different action mechanisms and adding a synergist, the permeability and surface tension of the agent can be increased, and the disease and pest resistance of crops can also be improved, enhancing the photosynthesis of crops, achieving the purpose of reducing the amount of pesticide used, and having the advantages of a long effective period and low residue. Summary of the Invention

[0006] The purpose of the present invention is to provide a synergistic suspending agent containing lufenuron, which can effectively control spinach Spodoptera exigua, increase the permeability and surface tension of the agent, improve the disease and pest resistance of crops, enhance the photosynthesis of crops, have a long effective period and low residue, and achieve the purpose of reducing the amount of pesticide used and environmental protection.

[0007] The technical solution of the present invention is:

[0008] A synergistic suspending agent containing lufenuron, characterized in that: based on 1000 parts by weight, the synergistic suspending agent comprises the following raw materials: 14-350 parts by weight of lufenuron, 10-250 parts by weight of indoxacarb, 10-200 parts by weight of synergist, 50-400 parts by weight of auxiliary agent, and the balance of water, wherein the weight ratio of lufenuron to indoxacarb is 7:5; optionally, based on 1000 parts by weight, the synergistic suspending agent comprises the following raw materials: 35-140 parts by weight of lufenuron, 25-100 parts by weight of indoxacarb, 20-100 parts by weight of synergist, 70-300 parts by weight of auxiliary agent, and the balance of water; optimally, based on 1000 parts by weight, the synergistic suspending agent comprises the following raw materials: 70 parts by weight of lufenuron, 50 parts by weight of indoxacarb, 30-80 parts by weight of synergist, 100-200 parts by weight of auxiliary agent, and the balance of water.

[0009] Further, the synergist is a mixture of a microbial metabolite and a high molecular emulsifier, wherein the weight ratio of the microbial metabolite to the high molecular emulsifier is, optionally the weight ratio is 5:1 to 1:3, and optionally the weight ratio is 3:1 to 1:1.

[0010] Further, the microbial metabolite is selected from one of erythritol, rhamnolipid, sophorolipid, and the high molecular emulsifier is selected from one of dodecylphenol polyoxyethylene ether or polyisobutylene anhydride;

[0011] Further, the auxiliary agent is selected from one or more of a dispersant, a wetting agent, an antifreeze, a thickener, an antifoaming agent, and a preservative.

[0012] The dispersant is selected from one or more of alkyl naphthalene sulfonates, alkyl naphthalene sulfonate formaldehyde polymers, lignin sulfonates, fatty alcohol polyoxyethylene ether phosphates, polycarboxylates, comb-shaped polycarboxylates, EO-PO block copolymers, and phenethylphenol polyoxyethylene ether phosphates;

[0013] The wetting agent is selected from one or more of alkyl sulfonates, alkyl naphthalene sulfonates, fatty alcohol polyoxyethylene ethers, alkyl glycosides, trisiloxane polyoxyethylene ethers, polyaryl phenolic polyoxyethylene ethers, and castor oil polyoxyethylene ethers;

[0014] The antifreeze is selected from one or more of ethylene glycol, propylene glycol, glycerol, polyethylene glycol, allantoin, and sorbitol;

[0015] The preservative is benzoic acid and its sodium salt, isothiazolinone, sorbic acid and its sodium or potassium salt;

[0016] The antifoaming agent is a silicone antifoaming agent, a C10-20 saturated fatty acid compound, or a C8-10 fatty alcohol;

[0017] The thickener described above is selected from one or more of xanthan gum, polyvinyl alcohol, magnesium aluminum silicate, sodium carboxymethyl cellulose, sodium alginate, and guar gum.

[0018] Furthermore, the total content of lufenuron and indoxacarb is 24 - 600 g / L, optionally 60 - 360 g / L, and optionally 120 g / L.

[0019] Furthermore, the application of the synergistic suspending agent in controlling Spodoptera exigua on spinach. When the synergistic suspending agent is used to control Spodoptera exigua on spinach, during the low - instar larval stage of Spodoptera exigua, it is sprayed once at an active ingredient dosage of 30 - 75 g / ha.

[0020] Compared with the prior art, the composition of the present invention has the following beneficial effects: (1) Compared with single agents, the composition has obvious synergistic effects on Spodoptera exigua on spinach, increases the permeability and surface tension of the pesticide, and significantly improves the control effect; (2) It is low - toxic and highly effective, reduces the amount of pesticide used, reduces the residue of pesticides on crops, and has a short safe interval; (3) It combines quick - acting and long - lasting effects, and has a good effect especially on resistant pests; (4) It improves the disease and pest resistance ability of crops and enhances the photosynthesis of crops. Detailed implementation modes

[0021] The following further illustrates the present invention in combination with embodiments, but the present invention is not limited thereto.

[0022] Application Example 1 The suspending composition disclosed in the following examples is calculated based on 1000 parts by weight and contains the following components: Example 1: 120 g / L lufenuron·indoxacarb suspending agent

[0023] 70 parts by weight of lufenuron technical, 50 parts by weight of indoxacarb technical, 30 parts by weight of erythritol, 10 parts by weight of dodecylphenol polyoxyethylene ether, 40 parts by weight of alkylnaphthalenesulfonate, 20 parts by weight of polycarboxylate, 25 parts by weight of alkyl glycoside, 60 parts by weight of ethylene glycol, 4 parts by weight of sodium benzoate, 2 parts by weight of C10 - 20 saturated fatty acid compounds, 3 parts by weight of magnesium aluminum silicate, and the balance is water, to obtain 120 g / L lufenuron·indoxacarb suspending agent.

[0024] Example 2: 120 g / L lufenuron·indoxacarb suspending agent

[0025] 70 parts by weight of lufenuron technical, 50 parts by weight of indoxacarb technical, 50 parts by weight of rhamnolipid, 20 parts by weight of polyisobutylene anhydride, 30 parts by weight of fatty alcohol polyoxyethylene ether phosphate, 15 parts by weight of phenethylphenol polyoxyethylene ether phosphate, 20 parts by weight of fatty alcohol polyoxyethylene ether, 60 parts by weight of glycerol, 5 parts by weight of isothiazolinone, 5 parts by weight of C8 - 10 fatty alcohols, 5 parts by weight of polyvinyl alcohol, and the balance is water, to obtain 120 g / L lufenuron·indoxacarb suspending agent.

[0026] Example 3: 120 g / L Chlorfluazuron and Indoxacarb Suspension Concentrate

[0027] 70 parts by weight of chlorfluazuron technical, 50 parts by weight of indoxacarb technical, 40 parts by weight of sophorolipid, 20 parts by weight of dodecylphenol polyoxyethylene ether, 40 parts by weight of comb-shaped polycarboxylate, 15 parts by weight of EO-PO block copolymer, 10 parts by weight of trisiloxane polyoxyethylene ether, 15 parts by weight of alkyl polyglycoside, 50 parts by weight of propylene glycol, 1 part by weight of xanthan gum, 3 parts by weight of silicone defoamer, 5 parts by weight of sodium benzoate, and the balance being water, to obtain 120 g / L chlorfluazuron and indoxacarb suspension concentrate.

[0028] Example 4: 240 g / L Chlorfluazuron and Indoxacarb Suspension Concentrate

[0029] 70 parts by weight of chlorfluazuron technical, 50 parts by weight of indoxacarb technical, 40 parts by weight of sophorolipid, 20 parts by weight of polyisobutenyl succinic anhydride, 30 parts by weight of alkylnaphthalenesulfonate formaldehyde polymer, 20 parts by weight of comb-shaped polycarboxylate, 30 parts by weight of fatty alcohol polyoxyethylene ether, 50 parts by weight of polyethylene glycol, 1 part by weight of xanthan gum, 3 parts by weight of silicone defoamer, 5 parts by weight of sorbic acid and its sodium salt, and the balance being water, to obtain 240 g / L chlorfluazuron and indoxacarb suspension concentrate.

[0030] Example 5: 360 g / L Chlorfluazuron and Indoxacarb Suspension Concentrate

[0031] 70 parts by weight of chlorfluazuron technical, 50 parts by weight of indoxacarb technical, 40 parts by weight of erythritol, 20 parts by weight of dodecylphenol polyoxyethylene ether, 30 parts by weight of phenethylphenol polyoxyethylene phosphate, 30 parts by weight of EO-PO block copolymer, 20 parts by weight of alkylnaphthalenesulfonate, 15 parts by weight of castor oil polyoxyethylene ether, 50 parts by weight of sorbitol, 5 parts by weight of magnesium aluminum silicate, 3 parts by weight of silicone defoamer, 5 parts by weight of sodium benzoate, and the balance being water, to obtain 360 g / L chlorfluazuron and indoxacarb suspension concentrate.

[0032] Example of Application 2:

[0033] Indoor joint toxicity determination experiment of chlorfluazuron, indoxacarb and their mixtures against Spodoptera exigua in spinach - Experiment purpose

[0034] To determine the toxicity of chlorfluazuron, indoxacarb and their different mixed combinations against Spodoptera exigua indoors, evaluate the synergistic effect, clarify their compatibility, and provide a scientific basis for the research and development of the mixture of chlorfluazuron and indoxacarb.

[0035] 2 Experimental conditions

[0036] 2.1 Test targets

[0037] The larvae of Spodoptera exigua (Hubner) were collected from the field and reared on spinach indoors for multiple generations. The 3rd instar larvae were used for bioassay experiments.

[0038] 2. Culture conditions

[0039] The culture conditions for the test targets and the targets after the test were a temperature of (25±1)°C, a relative humidity of 60% - 70%, and illumination (light - dark ratio 14:10).

[0040] 3. Experimental design

[0041] 3.1 Test chemicals

[0042] Lufenuron 98% technical material, Indoxacarb 95% technical material.

[0043] 3.2 Chemical preparation

[0044] Accurately weigh 1.0204 g of lufenuron with a ten - thousandth electronic balance, dissolve it with DMF and make up to 100 mL volumetric flask to prepare a 1×10 4 mg / L lufenuron stock solution for standby.

[0045] Accurately weigh 1.0526 g of indoxacarb with a ten - thousandth electronic balance, dissolve it with DMF and make up to 100 mL volumetric flask to prepare a 1×10 4 mg / L indoxacarb stock solution for standby.

[0046] Mix lufenuron and indoxacarb according to the following ratios: lufenuron:indoxacarb = 4:1, 3:1, 7:5, 1:2, and 1:3. Each ratio was also prepared into a 1×10 4 mg / L mixed stock solution for standby.

[0047] Based on the preliminary test, for each single chemical and different - ratio mixtures, 5 series of mass concentration treatments were set according to the active ingredient content. The specific treatment doses are as follows:

[0048] Lufenuron (A): 30, 15, 7.5, 3.75, 1.875 mg / L;

[0049] Indoxacarb (B): 14, 7.0, 3.5, 1.75, 0.875 mg / L;

[0050] A:B (4:1): 22, 11, 5.5, 2.75, 1.375 mg / L;

[0051] A:B (3:1): 20, 10, 5.0, 2.5, 1.25 mg / L;

[0052] A:B(7:5): 18, 9.0, 4.5, 2.25, 1.125 mg / L;

[0053] A:B(1:2): 16, 8.0, 4.0, 2.0, 1.0 mg / L;

[0054] A:B(1:3): 14, 7.0, 3.5, 1.75, 0.875 mg / L

[0055] 4 Test methods

[0056] Double filter paper method. Refer to Part 6: Leaf-dipping method (NY / T 1154.14 - 2008) and Part 7: Determination of combined effects of mixtures (NY / T 1154.7 - 2006) of the Agricultural Industry Standard of the People's Republic of China "Guidelines for Indoor Bioassay of Pesticides - Insecticides".

[0057] 5 Data investigation and statistical analysis

[0058] 5.1 Investigation time and method

[0059] After 96 h, check the mortality of the test insects and record it. The criterion for judging the death of the test insects is that if there is no response when gently touching the test insects with forceps, it is counted as dead.

[0060] 5.2 Evaluation method for synergistic effect

[0061] Refer to the Guidelines for Indoor Bioassay of Pesticides NY / T 1154.7 - 2006, and use the co-toxicity coefficient method of Sun & Johnson (1960) to calculate the co-toxicity coefficient (CTC) of each mixed combination. CTC ≤ 80 indicates antagonistic effect, 80 < CTC < 120 indicates additive effect, and CTC ≥ 120 indicates synergistic effect. The formula for calculating the co-toxicity coefficient is as follows:

[0062]

[0063] Theoretical toxicity index (TTI) of the mixture = Toxicity index of pesticide A × Percentage of pesticide A in the mixture (%) + Toxicity index of pesticide B × Percentage of pesticide B in the mixture (%)

[0064]

[0065] 6 Experimental results

[0066] Table 1 Toxicity determination results of the mixture of lufenuron and indoxacarb against Spodoptera exigua on spinach

[0067]

[0068] It can be seen from Table 1 of the test results that the test agent lufenuron has good toxicity to the 3rd instar larvae of Spodoptera exigua on spinach, with an LC 50 value of 7.5560 mg / L and an LC 90 value of 41.3847 mg / L; indoxacarb also has high toxicity, with an LC 50 value of 3.5404 mg / L and an LC 90 value of 18.9243 mg / L; the toxicities (LC 50 ) of the two agents mixed in different ratios to the 3rd instar larvae of Spodoptera exigua on spinach are 5.5592, 4.8018, 4.0036, 3.5606 and 3.5335 mg / L respectively, and the co-toxicity coefficients CTC reach 110.79, 122.60, 128.16, 120.84 and 115.55 respectively. It shows that the CTC values of lufenuron and indoxacarb are all greater than 120 within the range of 3:1 to 1:2 in the test design ratio, and there is a synergistic effect to varying degrees. Among them, the co-toxicity coefficient (CTC) of the 7:5 ratio is the largest and the synergistic effect is the most significant. Application Example 3: Field efficacy test of Examples 1-3 for controlling Spodoptera exigua on spinach 1 Test purpose

[0069] To verify the control effect of the 120 g / L lufenuron·indoxacarb suspension concentrate developed by the applicant on Spodoptera exigua on spinach and its safety to spinach, clarify the field application dosage and application technology, and provide a scientific basis for pesticide registration.

[0070] 2 Test basis

[0071] "Quality Management Specification for Pesticide Registration Tests", "GB / T 17980.13-2000 Pesticide Field Efficacy Test Guidelines (Part 1) Insecticides for Controlling Lepidopterous Larvae on Cruciferous Vegetables".

[0072] 3 Experimental site

[0073] This test is arranged in Shaanxi Province.

[0074] 4 Selection of test object, crop and variety

[0075] Test object: Spodoptera exigua on spinach;

[0076] 5 Test design and arrangement

[0077] 5.1 Dosage and number of agents

[0078] Table 2 Test design of test agents

[0079]

[0080] 5.2 Application time and frequency

[0081] During the occurrence period of the low-instar larvae of Spodoptera exigua on spinach, foliar spraying is used, and a total of 1 application is made.

[0082] 5.3 Application volume

[0083] The dosage of the liquid medicine is 750 liters per hectare.

[0084] 5.4 Investigation time and frequency

[0085] Investigate the initial population of insects before applying the medicine, and investigate the number of insects 1, 3, and 7 days after applying the medicine, for a total of 4 times.

[0086] 5.5 Calculation method of drug efficacy

[0087] The field drug efficacy is calculated according to the formula in the "Guidelines for Field Efficacy Trials of Pesticides" to calculate the control effect.

[0088]

[0089] PT: Control effect (%)

[0090] PT1: Number of live insects after applying the medicine in the medicament treatment area

[0091] PT0: Number of live insects before applying the medicine in the medicament treatment area

[0092] CK1: Number of live insects after applying the medicine in the blank control area

[0093] CK0: Number of live insects before applying the medicine in the blank control area

[0094] 6 Experimental results

[0095] Table 3 Results of field efficacy trials of Examples 1 - 3 for controlling Spodoptera exigua on spinach

[0096]

[0097]

[0098] The experimental results show that: the test substance of 120 g / L chlorfluazuron·indoxacarb suspension concentrate in the examples, for controlling Spodoptera exigua on spinach, is applied during the peak occurrence period of the low - instar larvae of Spodoptera exigua, and is sprayed at an active ingredient dosage of 54 g / ha. It can be seen from Table 3 that for the test drugs of Examples 1 - 3 of the present invention, when used to control Spodoptera exigua, 1 day, 3 days, and 7 days after applying the medicine, the control effects are significantly better than those of the control agents. After 7 days, the insecticidal effect reaches more than 89%, and some of the experimental results reach more than 95%. Compared with the control single agents, there are significant differences at the 0.01 and 0.05 levels. Therefore, it shows that the 120 g / L chlorfluazuron·indoxacarb suspension concentrate has quick - acting and long - lasting effects, and is safe for crops and applicators throughout the experimental period.

[0099] Application Example Four: Risk assessment of (Examples 1 - 3) and control single agents

[0100] Our company commissioned a third-party company to conduct residue determination on spinach for Examples 1 to 3 and a single control dose.

[0101] 1 Test basis

[0102] This test design refers to the "Guidelines for Testing for Pesticide Residues" (NY / T 788-2004) and is determined by referring to the methods specified in GB23200.8 and GB / T20769.

[0103] 2 Experimental location

[0104] This experiment was conducted in Shaanxi Province.

[0105] 3. Selection of experimental subjects, crops and varieties

[0106] Test subject: Spinach.

[0107] 4 Experimental design and arrangement

[0108] 4.1 Dosage and number of medicines

[0109] Table 5 Experimental design of test drugs

[0110]

[0111] 5.2 Application and extraction.

[0112] The pesticide was applied once, and 1 kg of fresh spinach leaves were collected 1 day, 3 days, 7 days and 14 days after the application. Random sampling was adopted, and the samples were extracted and then tested and analyzed by chromatography.

[0113] 6 Experimental results

[0114] Table 6 Results of the residual test of the tested agents on spinach

[0115]

[0116] It can be seen from Table 6 that, compared with the pesticide residue experiment on tomatoes of Examples 1-3 and the control single dose, the residue amount of the examples one day after the application is equivalent to that of the control single dose, slightly better than the control single dose, and the residue amount after 3 days, 7 days, and 14 days is significantly less than the residue amount of the control single dose, which makes the pesticide safety interval shorter and safer for consumers.

[0117] The above contents are further detailed descriptions of the present invention in combination with specific preferred embodiments, and it cannot be determined that the specific implementation of the present invention is limited to these descriptions. For ordinary technicians in the technical field to which the present invention belongs, several simple deductions or substitutions can be made without departing from the concept of the present invention, which should be regarded as falling within the protection scope of the present invention.

Claims

1. A synergistic suspending agent containing lufenuron, characterized in that: The synergistic suspending agent, based on 1000 parts by weight, comprises the following raw materials: 14 - 350 parts by weight of lufenuron, 10 - 250 parts by weight of indoxacarb, 10 - 200 parts by weight of synergist, 50 - 400 parts by weight of auxiliary agent, and the balance of water, wherein the weight ratio of lufenuron to indoxacarb is 7:

5.

2. The suspending agent according to claim 1, wherein: The synergist is a mixture of a microbial metabolite and a high - molecular emulsifier, wherein the weight ratio of the microbial metabolite to the high - molecular emulsifier is 3:1 to 1:3, and optionally the weight ratio is 2:1 to 1:

1.

3. The suspending agent according to claim 1 or 2, characterized in that: The synergistic suspending agent, based on 1000 parts by weight, comprises the following raw materials: 35 - 140 parts by weight of lufenuron, 25 - 100 parts by weight of indoxacarb, 20 - 100 parts by weight of synergist, 70 - 300 parts by weight of auxiliary agent, and the balance of water.

4. The suspending agent according to claim 3, characterized in that: The synergistic suspending agent, based on 1000 parts by weight, comprises the following raw materials: 70 parts by weight of lufenuron, 50 parts by weight of indoxacarb, 30 - 80 parts by weight of synergist, 100 - 200 parts by weight of auxiliary agent, and the balance of water.

5. The suspending agent according to any one of claims 1 to 4, characterized in that: The total content of lufenuron and indoxacarb is 24 - 600 g / L, optionally 60 - 240 g / L, and optionally 120 g / L.

6. The suspending agent according to claim 1, wherein: Application of the synergistic suspending agent in controlling Spodoptera exigua of spinach.

7. The suspending agent according to claim 6, characterized in that: When the synergistic suspending agent is used to control Spodoptera exigua of spinach, at the low - instar larval stage of Spodoptera exigua of spinach, it is sprayed once with an active ingredient dosage of 30 - 75 g / ha.

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