Application of suspension composition to prevention and treatment of cabbage pests

Abstract

The invention relates to the technical field of pesticides, and discloses application of a suspension composition to prevention and treatment of cabbage pests. The suspension composition contains lufenuron, cyantraniliprole, a biological synergist and an auxiliary agent, wherein the weight ratio of lufenuron to cyantraniliprole is (3: 1)-(1: 3). The suspension composition can effectively prevent and treat cabbage thrips, is safe and environment-friendly, can enhance crop immunity and crop quality, can prevent and treat pests, can enhance crop disease resistance, improves deposition adhesion and permeation of pesticides on leaf surfaces, improves the pesticide utilization rate, and has a good effect on preventing and treating cabbage thrips. Improvement of tolerance of crops to abiotic stress is the key to improvement of crop productivity.

Description

Technical Field

[0001] The present invention belongs to the technical field of pesticides, and particularly relates to the use of a suspension composition for controlling pests on cabbages. Background Art

[0002] Lufenuron belongs to benzoylurea insecticides and acaricides, and is also a chitin synthesis inhibitor, which affects the molting of pests and causes their death. It can also kill eggs and reduce the egg-laying amount of adults, and has stomach toxicity and contact toxicity. It is mainly used for controlling Lepidoptera pests on fruit trees, vegetables, cotton, corn and other crops.

[0003] Cyantraniliprole is a new type of amide systemic insecticide, mainly with stomach toxicity and also having contact toxicity. By activating the ryanodine receptor of target pests, it releases the calcium stored in striated muscle and smooth muscle cells, causing muscle contraction of pests, resulting in damage to muscle movement regulation, paralysis, and ultimately the death of pests.

[0004] The cabbage beet armyworm is a major pest on vegetables. It can damage various vegetables such as cabbages, Chinese cabbages, radishes, spinach, carrots, etc. It belongs to the Noctuidae family of Lepidoptera in insect classification. Another very common name is the cabbage noctuid.

[0005] It mainly damages the leaves of crops with larvae. When newly hatched, the larvae gather together and damage on the back of the leaves. They do not move during the day and feed on the leaves at night, leaving only the lower epidermis. When they reach the older stage (after the 4th instar), they lurk under the leaves, in the cabbage heart, on the ground surface or in the soil around the roots during the day and come out at night to form a gluttony. Seriously, they can often eat up the mesophyll, leaving only the veins and petioles. After eating one place, they migrate in groups to damage other places. In the case of cabbages, the larvae often drill into the leaf ball and leave a lot of feces, polluting the leaf ball and easily causing rot.

[0006] Because the beet armyworm has overlapping generations and can overwinter in various insect states, with strong viability and drug resistance, improving the productivity of crops on a warming earth and the tolerance to abiotic slopes is a major issue. To cope with environmental stresses such as drought, salinity, high temperature, and constant temperature, improving the tolerance of crops to abiotic stresses is the key to improving crop productivity.

[0007] When the yield is threatened by pathogens and pests, the currently mainly used products are still mainly chemical pesticides. Due to problems such as the product stability and long-lasting efficacy of biological pesticides, the amount used in practical applications is still very small. Therefore, how to improve the drug efficacy, environmental protection, safety, and stability to solve pest and disease problems will be an urgent problem to be solved in this technical field.

[0008] The applicant mixes two agents with completely different action mechanisms, adds a biological synergist, combines chemical pesticides with biological adjuvants, effectively improves the utilization rate of pesticides, solves the technical problem of insufficient stability of biological pesticides, enhances the immunity, quality and antibacterial ability of crops, and improves the tolerance of crops to abiotic stress. Summary of the Invention

[0009] The object of the present invention is to provide a use of a suspension composition for controlling Spodoptera exigua of cabbage, which can improve the tolerance of crops to abiotic stress, effectively improve the utilization rate of pesticides, solve the technical problem of insufficient stability of biological pesticides, and can also enhance the immunity, quality and antibacterial ability of crops.

[0010] The technical solution of the present invention is as follows:

[0011] A use of a suspension composition for controlling cabbage pests, characterized in that: the suspension composition contains lufenuron, cyantraniliprole, a biological synergist, and an adjuvant, and is made into a suspending agent, wherein the weight ratio of lufenuron to cyantraniliprole is 3:1 to 1:3.

[0012] Further, the weight ratio of lufenuron to cyantraniliprole in the suspending agent composition is 1:1.

[0013] Further, the content of lufenuron and cyantraniliprole in the suspending agent composition is 50 - 400 g / L, preferably 200 g / L.

[0014] Further, the biological synergist is a protein or its hydrolyzate, and the protein or its hydrolyzate can be obtained by extracting alcohol from by-products in biological pesticide fermentation, or can be a mixture of amino acids and peptides obtained by freeing proteins from plant or animal sources.

[0015] Further, the adjuvant is selected from one or more of a dispersant, a wetting agent, an antifreeze, a thickener, an antifoaming agent, a preservative, and water.

[0016] Further, based on 1000 parts by weight of the suspension composition, it comprises the following components:

[0017] Lufenuron, 10 - 200 parts by weight

[0018] Cyantraniliprole, 10 - 200 parts by weight

[0019] Biological synergist, 1 - 200 parts by weight

[0020] Dispersant, 15 - 75 parts by weight

[0021] Wetting agent, 15 - 75 parts by weight

[0022] Antifreeze, 0 - 60 parts by weight

[0023] Thickener, 2 - 25 parts by weight

[0024] Defoamer, 0.5 - 10 parts by weight

[0025] Preservative, 0 - 20 parts by weight

[0026] Water, the balance

[0027] Furthermore, based on 1000 parts by weight of the suspension composition, it comprises the following components:

[0028] Lufenuron, 50 - 150 parts by weight

[0029] Cyantraniliprole, 50 - 150 parts by weight

[0030] Bio - synergist, 5 - 150 parts by weight

[0031] Dispersant, 20 - 60 parts by weight

[0032] Wetting agent, 20 - 60 parts by weight

[0033] Antifreeze, 30 - 50 parts by weight

[0034] Thickener, 3 - 15 parts by weight

[0035] Defoamer, 0.5 - 5 parts by weight

[0036] Preservative, 0 - 15 parts by weight

[0037] Water, the balance

[0038] Furthermore, the dispersant is selected from one or more of alkyl naphthalene sulfonates, alkyl naphthalene sulfonate formaldehyde polymers, lignin sulfonates, polycarboxylates, EO - PO block copolymers; the wetting agent is selected from one or more of fatty alcohol polyoxyethylene ethers, alkylphenol polyoxyethylene ethers (nonylphenol polyoxyethylene ether and octylphenol polyoxyethylene ether), fatty amine polyoxyethylene ethers, alkyl glycosides, trisiloxane polyoxyethylene ethers, polyaryl phenol polyoxyethylene ethers, castor oil polyoxyethylene ethers, dodecyl benzene sulfonic acid; the antifreeze is selected from one or more of ethylene glycol, propylene glycol, glycerol; the preservative is sodium benzoate; the defoamer is silicone defoamers, C10 - 20 saturated fatty acid compounds, C8 - 10 fatty alcohol compounds; the thickener is selected from one or more of xanthan gum, polyvinyl alcohol, carboxymethyl cellulose.

[0039] Furthermore, the weight ratio of lufenuron: cyantraniliprole: bio - synergist is 8:8:(1 - 8).

[0040] Furthermore, the weight ratio of dispersant: wetting agent: antifreeze is (2 - 6):(1 - 4):(3 - 5).

[0041] Furthermore, the application rate of the active ingredient in the suspending agent composition is 10 - 60 g / ha, and the preferred application rate is 30 - 42 g / ha.

[0042] Compared with the prior art, the composition of the present invention has the following beneficial effects: (1) Compared with single agents, the composition has obvious synergistic effects on Spodoptera exigua of cabbage, improves the tolerance of crops to abiotic stress, and significantly improves the control effect; (2) It improves the penetration and adhesion of the liquid medicine, and improves the utilization rate of pesticides; (3) It is safe and environmentally friendly, with both quick-acting and long-acting properties; (4) It solves the problems of instability, difficult preservation, and short effective period of biopesticide products. Specific embodiments

[0043] The present invention will be further described below in conjunction with embodiments, but the present invention is not limited thereto.

[0044] In Application Example 1, the suspending composition is calculated based on 1000 parts by weight and contains the following components:

[0045] Example 1 200 g / L Chlorfluazuron · Cyantraniliprole Suspending Agent

[0046] 100 parts by weight of chlorfluazuron, 100 parts by weight of cyantraniliprole, 50 parts by weight of threonine, 50 parts by weight of arginine, 10 parts by weight of alkylnaphthalenesulfonate formaldehyde polymer, 15 parts by weight of polycarboxylate, 30 parts by weight of fatty alcohol polyoxyethylene ether, 40 parts by weight of propylene glycol, 5 parts by weight of xanthan gum, 8 parts by weight of sodium benzoate, 3 parts by weight of silicone defoamer, and the balance is water, to obtain 200 g / L chlorfluazuron · cyantraniliprole suspending agent.

[0047] Example 2 200 g / L Chlorfluazuron · Cyantraniliprole Suspending Agent

[0048] 100 parts by weight of chlorfluazuron, 100 parts by weight of cyantraniliprole, 100 parts by weight of glutamic acid, 20 parts by weight of EO-PO block copolymer, 12 parts by weight of alkylphenol polyoxyethylene ether, 12 parts by weight of alkyl glycoside, 45 parts by weight of ethylene glycol, 4 parts by weight of polyvinyl alcohol, 2 parts by weight of C8-10 fatty alcohols, 3 parts by weight of sodium benzoate, and the balance is water, to obtain 200 g / L chlorfluazuron · cyantraniliprole suspending agent.

[0049] Example 3 200 g / L Chlorfluazuron · Cyantraniliprole Suspending Agent

[0050] 100 parts by weight of lufenuron, 100 parts by weight of cyantraniliprole, 30 parts by weight of serrapeptase, 25 parts by weight of lignosulfonate, 5 parts by weight of EO-PO block copolymer, 15 parts by weight of trisiloxane polyoxyethylene ether, 25 parts by weight of alkyl polyglycoside, 40 parts by weight of glycerol, 4 parts by weight of carboxymethyl cellulose, 5 parts by weight of silicone defoamer, 7 parts by weight of sodium benzoate, and the balance being water, to prepare a 200 g / L lufenuron·cyantraniliprole suspension concentrate.

[0051] Example 4 100 g / L lufenuron·cyantraniliprole suspension concentrate

[0052] 50 parts by weight of lufenuron, 50 parts by weight of cyantraniliprole, 25 parts by weight of keratinase, 15 parts by weight of alkylnaphthalenesulfonate, 15 parts by weight of polyaryl phenol polyoxyethylene ether, 50 parts by weight of glycerol, 5 parts by weight of xanthan gum, 2 parts by weight of C10-20 saturated fatty acid compounds, 10 parts by weight of sodium benzoate, and the balance being water, to prepare a 100 g / L lufenuron·cyantraniliprole suspension concentrate.

[0053] Example 5 400 g / L lufenuron·cyantraniliprole suspension concentrate

[0054] 200 parts by weight of lufenuron, 200 parts by weight of cyantraniliprole, 125 parts by weight of phenylalanine, 20 parts by weight of polycarboxylate, 20 parts by weight of EO-PO block copolymer, 40 parts by weight of dodecylbenzenesulfonic acid, 50 parts by weight of ethylene glycol, 5 parts by weight of polyvinyl alcohol, 1.5 parts by weight of silicone defoamer, 7 parts by weight of sodium benzoate, and the balance being water, to prepare a 400 g / L lufenuron·cyantraniliprole suspension concentrate.

[0055] Example Application 2:

[0056] Indoor joint toxicity determination experiment of lufenuron, cyantraniliprole and their mixtures against Spodoptera exigua (Hübner) on cabbage

[0057] 1 Test purpose

[0058] To determine the toxicity of lufenuron and cyantraniliprole and their different mixing combinations against Spodoptera exigua (Hübner) on cabbage indoors, evaluate the synergistic effect, clarify their compatibility, and provide a scientific basis for the research and development of the mixture of lufenuron and cyantraniliprole.

[0059] 2 Experimental conditions

[0060] 2.1 Test target

[0061] Spodoptera exigua (Hübner), and the test target used was the adults that occurred naturally and reproduced for multiple generations on cabbage planted in the test base without prior pesticide application.

[0062] 2. Culture conditions

[0063] The culture conditions for the test target and the target after the test are a temperature of (25±1)°C, a relative humidity of 60% - 80%, and a light cycle of L∶D = (16∶8) h.

[0064] 3 Experimental Design

[0065] 3.1 Test Chemicals

[0066] Lufenuron 98% technical; Cyantraniliprole 94% technical.

[0067] 3.2 Chemical Preparation

[0068] Weigh 0.0102 g of lufenuron 98% technical, dissolve it in 2 mL of acetone, add 0.2 mL of Tween 80 emulsifier, stir evenly, add water to 100 mL to prepare a stock solution of 100 mg / L. Then take 40 mL of the stock solution and add it to 60 mL of water containing 0.1% Tween 80 emulsifier to prepare a test solution of 40 mg / L. Then dilute it with water containing 0.1% Tween 80 emulsifier by a factor of 2 to 20, 10, 5, 2.5, and 1.25 mg / L, and conduct tests at a total of 6 concentrations.

[0069] Weigh 0.0106 g of cyantraniliprole 94% technical, dissolve it in 2 mL of acetone, add 0.2 mL of Tween 80 emulsifier, stir evenly, add water to 100 mL to prepare a stock solution of 100 mg / L. Then take 20 mL of the stock solution and add it to 80 mL of water containing 0.1% Tween 80 emulsifier to prepare a test solution of 40 mg / L. Then dilute it with water containing 0.1% Tween 80 emulsifier by a factor of 2 to 10, 5, 2.5, 1.25, and 0.625 mg / L, and conduct tests at a total of 6 concentrations.

[0070] According to the ratios of lufenuron to cyantraniliprole of 3∶1, 2∶1, 1∶1, 1∶2, and 1∶3, respectively measure 15, 13.33, 10, 6.67, and 5 mL of the lufenuron stock solution, and then correspondingly measure 5, 6.67, 10, 13.33, and 15 mL of the cyantraniliprole stock solution. Mix the two, and respectively make up the water with emulsifier added to 100 mL to prepare test solutions of 40 mg / L. Then dilute them with water containing 0.1% Tween 80 emulsifier by a factor of 2 to 6 concentrations each for testing.

[0071] Add water containing 0.1% Tween 80 emulsifier to 100 mL in a beaker containing 2 mL of acetone as the blank control.

[0072] 4 Test Methods

[0073] Refer to the Guidelines for Pesticide Bioassays in the Laboratory NY / T 1154.14-2008 and use the leaf disc immersion method. Use a punch with a diameter of 18 mm to punch out leaf discs from cabbage leaves. After immersing the leaf discs in the test liquid for 10 seconds, place them in a petri dish (90 mm in diameter) lined with moist filter paper. Put 5 leaf discs in each dish. After natural drying, select 2nd instar larvae of Spodoptera exigua that have been starved for 4 h and put them into the petri dish, with no less than 10 larvae in each dish. Repeat 4 times. After the treatment, cover the dish lid and place it in the observation room for cultivation.

[0074] 5 Data Investigation and Statistical Analysis

[0075] 5.1 Investigation Time and Method

[0076] After 96 h, check the mortality of the test insects and record it. The criterion for judging the death of the test insects is that if there is no response when gently touching the test insects with a pointed writing brush, it is counted as dead.

[0077] 5.2 Data Statistical Analysis

[0078] Refer to the Guidelines for Pesticide Bioassays in the Laboratory NY / T 1154.7-2006 and use the co-toxicity coefficient method of Sun & Johnson (1960) to calculate the co-toxicity coefficient (CTC) of each mixed formulation. CTC ≤ 80 indicates antagonistic effect, 80 < CTC < 120 indicates additive effect, and CTC ≥ 120 indicates synergistic effect. The formula for calculating the co-toxicity coefficient is as follows:

[0079]

[0080] The theoretical toxicity index (TTI) of the mixture = the toxicity index of pesticide A × the percentage of pesticide A in the mixture (%) + the toxicity index of pesticide B × the percentage of pesticide B in the mixture (%)

[0081]

[0082] 6 Experimental Results

[0083] The results of the toxicity determination of the mixtures of lufenuron, cyantraniliprole and their mixtures at the ratios of 3:1, 2:1, 1:1, 1:2 and 1:3 against Spodoptera exigua on cabbage are shown in Table 1. It can be seen from the table that the mixtures of lufenuron and cyantraniliprole at the above 5 ratios all showed synergistic effects against Spodoptera exigua on cabbage, and their LC 50 values were 3.78, 3.39, 2.50, 2.46 and 2.28 mg / L respectively, and the co-toxicity coefficients were 125.63, 129.70, 153.16, 137.85 and 140.69 respectively. Among them, the synergistic effect of the 1:1 ratio was the most significant.

[0084] Table 1 Results of the toxicity determination of the mixtures of lufenuron and cyantraniliprole against Spodoptera exigua on cabbage

[0085]

[0086] The results of this indoor bioassay showed that the mixing ratios of lufenuron and cyantraniliprole in the range of 3:1, 2:1, 1:1, 1:2 and 1:3 all showed synergistic effects on Spodoptera exigua, among which the synergistic effect of 1:1 was the most significant. Therefore, from the comparison of synergistic toxicity alone, 1:1 can be regarded as the optimal mixing ratio of lufenuron and cyantraniliprole.

[0087] Application Example 3: Example Field Efficacy Test for Controlling Cabbage Beet Armyworm 1 Test Purpose

[0088] To verify the control effect of the 200 g / L lufenuron-cyantraniliprole suspension developed by the applicant on cabbage beet armyworm and its safety to cabbage, to clarify the field dosage and application technology, and to provide a scientific basis for pesticide registration.

[0089] 2 Test basis

[0090] "Quality Management Specification for Pesticide Registration Tests", "GB / T 17980.13-2000 National Standard of the People's Republic of China - Guidelines for Field Efficacy Tests of Pesticides (I) Insecticides for Control of Lepidoptera Larvae on Cruciferous Vegetables", and "NWP-SOP-I-009 Field Test Operating Procedures for Lepidoptera Larvae on Cruciferous Vegetables".

[0091] 3 Experimental location

[0092] This experiment was arranged in Shaanxi Province and Hainan Province.

[0093] 4. Selection of experimental subjects, crops and varieties

[0094] Test subjects: Cabbage beet armyworm;

[0095] Experimental crops: Cabbage.

[0096] 5 Experimental design and arrangement

[0097] 5.1 Dosage and number of medicines

[0098] Table 2 Experimental design of test drugs

[0099]

[0100]

[0101] 5.2 Application time and frequency

[0102] During the early larval stage of cabbage beet armyworm, a manual backpack sprayer was used for spraying, with a total of 1 application.

[0103] 5.3 Usage capacity

[0104] Spray 50 liters of liquid medicine per mu, and 750 liters of liquid medicine per hectare.

[0105] 5.4 Investigation time and frequency

[0106] Investigate the base number before applying the medicine, and conduct efficacy investigations 1 day, 3 days, 7 days, and 10 days after applying the medicine. A total of 5 investigations are conducted.

[0107] 5.5 Method for calculating the efficacy of the medicine

[0108]

[0109]

[0110] 6 Experimental results

[0111]

[0112] It can be seen from the experimental results that for the experimental medicament 200 g / L lufenuron·cyantraniliprole suspension concentrate and the control single agent in controlling Plutella xylostella on cabbage, in Examples 1, 2, and 3, when the active ingredient dosage is 30 g / hm 2 、42 g / hm 2 , the control effect is significantly better than that of the control single agent. Analysis of variance shows that: the efficacy differences are significant at the 0.05 and 0.01 levels 3 days, 7 days, and 10 days after applying the medicine, and are significantly better than the control single agent.

[0113] To sum up, the 200 g / L lufenuron·cyantraniliprole suspension concentrate requested by the applicant for protection has a good control effect on Spodoptera exigua on cabbage. Considering the differences among different dosages after applying the medicine, it is recommended to spray the medicine in the field at the initial stage of the occurrence of Spodoptera exigua on cabbage, and the recommended dosage is 30 - 42 g(a.i.) / ha.

[0114] The above content is a further detailed description of the present invention in combination with specific preferred implementation manners. It cannot be determined that the specific implementation of the present invention is only limited to these descriptions. For those of ordinary skill in the technical field to which the present invention belongs, without departing from the concept of the present invention, several simple deductions or substitutions can still be made, and all should be regarded as belonging to the protection scope of the present invention.

Claims

1. Use of a suspension composition for controlling pests on cabbages, characterized in that: The suspension composition contains lufenuron, cyantraniliprole, a biological synergist, and an adjuvant, and is made into a suspension concentrate. The weight ratio of lufenuron to cyantraniliprole is 3:1 to 1:

3.

2. The use according to claim 1, wherein: In the suspension concentrate composition, the weight ratio of lufenuron to cyantraniliprole is 1:

1.

3. The use according to claim 2, wherein: In the suspension concentrate composition, the content of lufenuron and cyantraniliprole is 50 - 400 g / L.

4. The use according to claim 3, characterized in that: The content of the effective active ingredient in the pesticide composition is 200 g / L.

5. The use according to claim 1, wherein: The said biological synergist is a protein or its hydrolyzate.

6. The use according to claim 1, wherein: The said adjuvant is selected from one or more of a dispersant, a wetting agent, an antifreezing agent, a thickening agent, an antifoaming agent, a preservative, and water.

7. Use according to any one of claims 1 to 6, characterized in that: Based on 1000 parts by weight, the suspension composition comprises the following components: Lufenuron, 10 - 200 parts by weight Cyantraniliprole, 10 - 200 parts by weight Biological synergist, 1 - 200 parts by weight Dispersant, 15 - 75 parts by weight Wetting agent, 15 - 75 parts by weight Antifreezing agent, 0 - 60 parts by weight Thickening agent, 2 - 25 parts by weight Antifoaming agent, 0.5 - 10 parts by weight Preservative, 0 - 20 parts by weight Water, the balance.

8. The use according to claim 7, characterized in that: Based on 1000 parts by weight, the suspension composition comprises the following components: Lufenuron, 50 - 150 parts by weight Cyantraniliprole, 50 - 150 parts by weight Biological synergist, 5 - 150 parts by weight Dispersant, 20 - 60 parts by weight Wetting agent, 20 - 60 parts by weight Antifreezing agent, 30 - 50 parts by weight Thickening agent, 3 - 15 parts by weight Antifoaming agent, 0.5 - 5 parts by weight Preservative, 0 - 15 parts by weight Water, the balance.

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