Anthocyanidin-containing berry fruit extract composition and application thereof in eye protection

Abstract

The invention provides a blueberry fruit extract composition containing anthocyanin. The composition has the effects of eliminating free radicals, resisting oxidation, protecting eyes, inhibiting tear secretion reduction, relieving eyestrain and eye dryness, repairing retina, protecting eyesight, preventing and controlling myopia, improving eye blood circulation and the like.

Description

Technical Field

[0001] The present invention belongs to the field of health products and relates to a berry extract composition containing anthocyanins. This composition has the functions of eliminating free radicals, antioxidation, protecting eyes, inhibiting the decrease of tear secretion ability, relieving eye fatigue and dryness, repairing the retina, protecting eyesight, preventing and controlling myopia, and improving blood circulation in the eyes. Background Art

[0002] With the continuous development of information technology and the accelerating popularization of electronic products, people's exposure time to various electronic products such as computers and mobile phones is constantly increasing, and the resulting eye health problems such as myopia, eye fatigue, and eye dryness are becoming increasingly serious. The blue light of the electronic screen can directly penetrate the lens of the human eye and reach the retina, causing atrophy and even death of retinal pigment epithelial cells. In addition, the complications caused by some chronic diseases will also have an adverse impact on the eyes, making eye problems more common and intractable. Thus, eye-related health problems need to receive greater attention.

[0003] The retina is one of the most metabolically active and oxygen-consuming tissues in the human body. It is rich in unsaturated fatty acids. When the eyes are exposed to light for a long time, especially the blue light emitted by the electronic screen, the retina is prone to oxidative stress and generate endogenous reactive oxygen species (ROS). ROS can react with mitochondria, lipids, proteins, nucleic acids, etc. in the macula, damage vascular wall cells, and cause irreversible damage to the retina.

[0004] At the same time, long-term and close-range eye use for learning and / or work also causes great damage to the eyes. It will cause the ciliary muscle to be tense, resulting in ciliary muscle fatigue and thus leading to myopia.

[0005] Anthocyanin is a powerful free radical scavenger. It has the ability to resist free radical oxidation, can inhibit the activity of development and DNA damage response regulator protein 1 (REDD1 protein), relieve oxidative stress, and protect the blood-retinal barrier; it can eliminate the free radicals that cause vascular sclerosis, prevent arteriosclerosis; increase the flexibility of capillaries, purely promote blood vessel expansion and elasticity, and prevent blood vessel rupture; strengthen the capillaries in the eyes to achieve the effect of preventing vascular lesions; it has the effects of protecting eyes, inhibiting the decrease of tear secretion ability, relieving eye fatigue and dryness, protecting eyesight, improving myopia and macular degeneration, and preventing blindness, glaucoma, cataract, retinal hemorrhage, night blindness, etc. Summary of the Invention

[0006] The purpose of the present invention is to provide a berry extract composition containing anthocyanins. This composition has the functions of eliminating free radicals, antioxidation, protecting eyes, inhibiting the decrease of tear secretion ability, relieving eye fatigue and dryness, repairing the retina, protecting eyesight, preventing and controlling myopia, and improving blood circulation in the eyes.

[0007] The composition of the present invention has the following advantages:

[0008] 1. It has the effects of improving eyesight, anti - inflammation, antibacterial, antioxidant, etc., and has high bioavailability and high absorption rate;

[0009] 2. It has strong free - radical scavenging ability, can repair mitochondria, inhibit the decrease of tear secretion ability, and relieve eye fatigue and dryness;

[0010] 3. It can moisten the eyes for a long time, reach the root of the lacrimal gland, restore the normal secretion of lacrimal gland cells, specifically protect the lacrimal gland, and solve eye dryness and eye fatigue from the root;

[0011] 4. It can effectively improve the damage of blue light to the structure and cells of the retina and repair the retina;

[0012] 5. It can protect eyesight, prevent and control myopia, and improve blood circulation in the eyes.

[0013] The composition of the present invention is targeted at adolescent student population.

[0014] The composition of the present invention is targeted at people who use their eyes for a long time.

[0015] The composition of the present invention is targeted at working population performing visual display terminal (VDT) tasks.

[0016] The composition of the present invention is targeted at people with eye discomfort.

[0017] The composition of the present invention is targeted at children population.

[0018] The present invention provides a berry extract composition containing anthocyanins, wherein the source of the anthocyanins is one or more of blackcurrant extract, aronia berry extract, bilberry extract, maqui berry extract, blueberry extract, acai berry extract, blackberry extract.

[0019] In one embodiment, for the berry extract composition of the present invention, the source of the anthocyanins is one or more of blackcurrant extract, aronia berry extract, bilberry extract, maqui berry extract, blueberry extract.

[0020] In one embodiment, for the berry extract composition of the present invention, the source of the anthocyanins is blackcurrant extract, aronia berry extract, bilberry extract, maqui berry extract, blueberry extract.

[0021] In one embodiment, for the berry extract composition of the present invention, the berry extract is a composition of blackcurrant extract, aronia berry extract, bilberry extract, maqui berry extract.

[0022] In one embodiment, for the berry extract composition of the present invention, the anthocyanins include one or more of delphinidin, cyanidin, pelargonidin, peonidin, petunidin, and malvidin.

[0023] In one embodiment, for the berry extract composition of the present invention, the anthocyanins exist in glycoside form.

[0024] In one embodiment, for the berry extract composition of the present invention, the glycosyl groups of the anthocyanins include one or more of glucose, galactose, rutinose, sambubiose, arabinose, xylose, sambubioside, and cellobiose.

[0025] In one embodiment, for the berry extract composition of the present invention, the content of blackcurrant extract is ≥1%.

[0026] In a preferred embodiment, for the berry extract composition of the present invention, the content of blackcurrant extract is ≥5%, such as 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25%.

[0027] In a preferred embodiment, for the berry extract composition of the present invention, the content of blackcurrant extract is 5 - 15%.

[0028] In a more preferred embodiment, for the berry extract composition of the present invention, the content of blackcurrant extract is 5 - 10%.

[0029] In one embodiment, for the berry extract composition of the present invention, the content of anthocyanins in the blackcurrant extract is ≥10%, preferably the content of anthocyanins is ≥20%, and more preferably the content of anthocyanins is ≥30%.

[0030] In one embodiment, for the berry extract composition of the present invention, the content of chokeberry extract is ≥10%.

[0031] In a preferred embodiment, for the berry extract composition of the present invention, the content of the aronia extract is ≥ 20%. Such as 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86, 87, 88, 89, 90%.

[0032] In a preferred embodiment, for the berry extract composition of the present invention, the content of the aronia extract is 20 - 80%.

[0033] In a more preferred embodiment, for the berry extract composition of the present invention, the content of the aronia extract is 50 - 80%.

[0034] In a particularly preferred embodiment, for the berry extract composition of the present invention, the content of the aronia extract is 65 - 75%.

[0035] In an embodiment, for the berry extract composition of the present invention, the content of anthocyanins in the aronia extract is ≥ 5%, preferably the content of anthocyanins is ≥ 10%, and more preferably the content of anthocyanins is ≥ 20%.

[0036] In an embodiment, for the berry extract composition of the present invention, the content of anthocyanins in the bilberry extract is ≥ 10%, preferably the content of anthocyanins is ≥ 20%, and more preferably the content of anthocyanins is ≥ 30%.

[0037] In an embodiment, for the berry extract composition of the present invention, the content of the maqui berry extract is ≥ 1%. Such as 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30%.

[0038] In a preferred embodiment, for the berry extract composition of the present invention, the content of the maqui berry extract is ≥ 10%.

[0039] In a preferred embodiment, for the berry extract composition of the present invention, the content of the maqui berry extract is 10 - 25%.

[0040] In a more preferred embodiment, in the berry extract composition of the present invention, the content of the maqui berry extract is 15-25%.

[0041] In one embodiment, in the berry extract composition of the present invention, the content of anthocyanins in the maqui berry extract is ≥20%, preferably the content of anthocyanins is ≥40%, and more preferably the content of anthocyanins is ≥60%.

[0042] In one embodiment, in the berry extract composition of the present invention, the content ratio of the blackcurrant extract to the maqui berry extract is 9:1 to 1:9.

[0043] In a preferred embodiment, in the berry extract composition of the present invention, the content ratio of the blackcurrant extract to the maqui berry extract is 5:1 to 1:9.

[0044] In a more preferred embodiment, in the berry extract composition of the present invention, the content ratio of the blackcurrant extract to the maqui berry extract is 2:1 to 1:9.

[0045] In a particularly preferred embodiment, in the berry extract composition of the present invention, the content ratio of the blackcurrant extract to the maqui berry extract is 1:1 to 1:5.

[0046] In one embodiment, in the berry extract composition of the present invention, the active ingredients of the blackcurrant extract are one or more of delphinidin-3-O-glucoside, delphinidin-3-O-rutinoside, cyanidin-3-O-glucoside, and cyanidin-3-O-rutinoside.

[0047] In one embodiment, in the berry extract composition of the present invention, the active ingredients of the chokeberry extract are one or more of cyanidin-3-O-galactoside, cyanidin-3-O-glucoside, cyanidin-3-O-arabinoside, and cyanidin-3-xyloside.

[0048] In one embodiment, in the berry extract composition of the present invention, the active ingredients of the maqui berry extract are one or more of delphinidin-3-sambubioside-glucoside, delphinidin-3,5-diglucoside, cyanidin-3-sambubioside-5-glucoside, cyanidin diglucoside, delphinidin-3-sambubioside, delphinidin-3-glucoside, cyanidin-3-sambubioside chloride, and cyanidin-3-O-glucoside.

[0049] In one embodiment, in the berry extract composition of the present invention, the active ingredients of the blackcurrant extract at least include delphinidin-3-O-glucoside.

[0050] In one embodiment, for the berry extract composition of the present invention, the active ingredients of the maqui berry extract include at least one or more of delphinidin-3-O-glucoside, delphinidin-3-sambubioside, and delphinidin-3,5-diglucoside.

[0051] In one embodiment, for the berry extract composition of the present invention, the active ingredients of the berry extract composition include at least one or more of delphinidin-3-O-glucoside, delphinidin-3-sambubioside, delphinidin-3,5-diglucoside, delphinidin-3-sambubioside, delphinidin-3-O-rutinoside, cyanidin-3-O-galactoside, cyanidin-3-O-glucoside, cyanidin-3-O-arabinoside, cyanidin-3-O-rutinoside, cyanidin-3-sambubioside-5-glucoside, and cyanidin diglucoside.

[0052] In some embodiments, for the berry extract composition of the present invention, the content of delphinidin in the berry extract is ≥5%. Such as 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20%.

[0053] In a preferred embodiment, for the berry extract composition of the present invention, the content of delphinidin in the berry extract is ≥10%.

[0054] In a more preferred embodiment, for the berry extract composition of the present invention, the content of delphinidin in the berry extract is ≥15%.

[0055] In a particularly preferred embodiment, for the berry extract composition of the present invention, the content of delphinidin in the berry extract is 10 - 20%.

[0056] In some embodiments, for the berry extract composition of the present invention, the active ingredients of the berry extract composition include at least delphinidin-3-O-glucoside; the content of delphinidin-3-O-glucoside in the composition is ≥1%. Such as 1, 2, 3, 4, 5, 6, 7, 8, 9, 10%.

[0057] In a preferred embodiment, for the berry extract composition of the present invention, the content of delphinidin-3-O-glucoside in the berry extract composition is ≥2%.

[0058] In a more preferred embodiment, for the berry extract composition of the present invention, the content of delphinidin-3-O-glucoside in the berry extract composition is 2 - 5%.

[0059] In one embodiment, for the berry extract composition of the present invention, the active ingredients of the berry extract composition include at least one or more of delphinidin-3-O-glucoside, delphinidin-3-sambubioside, and delphinidin-3,5-diglucoside.

[0060] In one embodiment, for the berry extract composition of the present invention, the active ingredients of the berry extract composition include at least one or more of delphinidin-3-glucoside and delphinidin-3,5-diglucoside.

[0061] In some embodiments, for the berry extract composition of the present invention, the content of delphinidin-3-sambubioside in the berry extract composition is ≥1%. Such as 1, 2, 3, 4, 5, 6, 7%.

[0062] In some embodiments, for the berry extract composition of the present invention, the content of delphinidin-3,5-diglucoside in the berry extract composition is ≥1%. Such as 1, 2, 3, 4, 5%.

[0063] In a preferred embodiment, for the berry extract composition of the present invention, the content of delphinidin-3-sambubioside in the berry extract composition is ≥2%.

[0064] In a more preferred embodiment, for the berry extract composition of the present invention, the content of delphinidin-3-sambubioside in the berry extract composition is 2 - 5%.

[0065] In a preferred embodiment, for the berry extract composition of the present invention, the content of delphinidin-3,5-diglucoside in the berry extract composition is ≥2%.

[0066] In a more preferred embodiment, for the berry extract composition of the present invention, the content of delphinidin-3,5-diglucoside in the berry extract composition is 2 - 5%.

[0067] In some embodiments, for the berry extract composition of the present invention, the anthocyanin content in the berry extract composition is ≥10%. Such as 10, 15, 20, 25, 30, 35, 40, 45, 50%.

[0068] In a preferred embodiment, in some embodiments, for the berry extract composition of the present invention, the anthocyanin content in the berry extract composition is ≥20%.

[0069] In a more preferred embodiment, in some embodiments, for the berry extract composition of the present invention, the anthocyanin content of the berry extract composition is ≥ 30%.

[0070] In one embodiment, the preparation method of the berry extract (such as blackcurrant extract, aronia berry extract, bilberry extract, maqui berry extract, blueberry extract, etc.) specifically includes: (1). Select and process plant raw materials, such as cleaning, cutting or pulverizing;

[0071] (2). Extract active ingredients by enzymatic hydrolysis method, water extraction method, alcohol extraction method, ultrasonic extraction method, supercritical fluid extraction method, etc.;

[0072] (3). Separate and purify the extract by methods such as filtration, centrifugation, chromatography, membrane separation and crystallization;

[0073] (4). Concentrate and dry the extract by techniques such as evaporation, freeze-drying or spray-drying.

[0074] The present invention provides an anthocyanin-containing berry extract composition, which includes but is not limited to powder, granule, syrup, solution, gel form. The berry extract composition is prepared with excipients into one or more of tablets, soft capsules, capsules, powders, oral liquids, sprays, solid beverages, crystal balls, gummies, jellies, popping candies.

[0075] The present invention also provides the use of any of the above-mentioned anthocyanin-containing berry extract compositions in scavenging free radicals, antioxidant, protecting eyes, inhibiting the decrease of tear secretion ability, relieving eye fatigue and dryness, repairing the retina, protecting eyesight, preventing and controlling myopia, improving ocular blood circulation, etc.

[0076] The present invention also provides the use of any of the above-mentioned anthocyanin-containing berry extract compositions in protecting eyes.

[0077] The present invention also provides the use of any of the above-mentioned anthocyanin-containing berry extract compositions in repairing the retina.

[0078] The present invention also provides the use of any of the above-mentioned anthocyanin-containing berry extract compositions in inhibiting the decrease of tear secretion ability.

[0079] The present invention also provides the use of any of the above-mentioned anthocyanin-containing berry extract compositions in relieving eye fatigue and dryness.

[0080] The present invention also provides the use of any of the above-mentioned anthocyanin-containing berry extract compositions in protecting eyesight, preventing and controlling myopia, and improving ocular blood circulation.

[0081] The present invention provides a composition of berry extract containing anthocyanins for eye protection, wherein the anthocyanins are derived from one or more of blackcurrant extract, aronia berry extract, bilberry extract, maqui berry extract, blueberry extract, acai berry extract, and blackberry extract.

[0082] In one embodiment, the composition containing anthocyanins for eye protection according to the present invention comprises any one of the above compositions. BRIEF DESCRIPTION OF THE DRAWINGS

[0083] Figure 1 It is a diagram of the retinal structure and cell morphology.

[0084] Figure 2 It is a diagram of the thickness of the outer nuclear layer (ONL) of the retina.

[0085] Figure 3 It is a diagram of the expression of Nrf2 protein in the mouse eyeball. DETAILED DESCRIPTION OF THE INVENTION

[0086] For the convenience of those skilled in the art to understand, the present invention will be further described below in conjunction with embodiments. The content mentioned in the embodiments does not limit the present invention.

[0087] Embodiment

[0088] A composition is obtained by adding blackcurrant extract, aronia berry extract, bilberry extract, maqui berry extract, and blueberry extract.

[0089] Example 1 Example 2 Example 3 Example 4 Example 5 Blackcurrant extract (mg) 2.16 2.29 2.43 2.16 2.43 Aronia extract (mg) 18.90 20.87 18.90 18.77 18.90 Bilberry extract (mg) 0.27 0.27 0.27 0.27 0.27 Maqui berry extract (mg) 2.16 3.44 5.40 5.67 5.40 Blueberry extract (mg) 0.13 0.13 0 0.13 0.13

[0090] Embodiment 6

[0091] A composition is obtained by adding blackcurrant extract, aronia berry extract, bilberry extract, maqui berry extract, and blueberry extract according to any one of the addition amounts in Embodiments 1-5, wherein the anthocyanin content in the above composition is 30%.

[0092] Embodiment 7

[0093] A composition is obtained by adding blackcurrant extract, aronia berry extract, bilberry extract, maqui berry extract, and blueberry extract according to any one of the addition amounts in Embodiments 1-5, wherein the anthocyanin content in the above composition is 35%.

[0094] Embodiment 8

[0095] A composition is obtained by adding blackcurrant extract, aronia berry extract, bilberry extract, maqui berry extract, and blueberry extract according to any one of the addition amounts in Embodiments 1-5, wherein the anthocyanin content in the above composition is 25%.

[0096] Embodiment 9

[0097] Add blackcurrant extract, chokeberry extract, bilberry extract, maqui berry extract, and blueberry extract according to any one of the addition amounts in Examples 1-5 to obtain a composition, wherein the anthocyanin content in the above composition is 40%.

[0098] Example 10

[0099] Add blackcurrant extract, chokeberry extract, bilberry extract, maqui berry extract, and blueberry extract according to any one of the addition amounts in Examples 1-5 to obtain a composition, wherein the delphinidin content in the above composition is 10%.

[0100] Example 11

[0101] Add blackcurrant extract, chokeberry extract, bilberry extract, maqui berry extract, and blueberry extract according to any one of the addition amounts in Examples 1-5 to obtain a composition, wherein the delphinidin content in the above composition is 15%.

[0102] Example 12

[0103] Mix blackcurrant extract, chokeberry extract, bilberry extract, maqui berry extract, and blueberry extract, add excipients, mix evenly, pour it into a capsule mold to prepare capsules. The preparation method includes mixing blackcurrant extract, chokeberry extract, bilberry extract, maqui berry extract, and blueberry extract, filling them into capsules, sealing them by dry sealing or wet sealing, and finally performing capsule packaging.

[0104] Example 13

[0105] Mix blackcurrant extract, chokeberry extract, bilberry extract, maqui berry extract, and blueberry extract, and then add excipients such as maltodextrin to make a powder stick pack containing anthocyanins. The preparation method includes mixing blackcurrant extract, chokeberry extract, bilberry extract, maqui berry extract, and blueberry extract with other excipients, making them into a powder by spray drying, and then filling them into stick packs.

[0106] Example 14

[0107] Mix blackcurrant extract, chokeberry extract, bilberry extract, maqui berry extract, and blueberry extract, add appropriate stabilizers and preservatives such as glycerol and potassium sorbate to make an oral liquid. The preparation method includes dissolving blackcurrant extract, chokeberry extract, bilberry extract, maqui berry extract, and blueberry extract, mixing them evenly, adjusting the pH value, filtering and sterilizing, and filling them into sterile bottles and sealing.

[0108] Example 15

[0109] Mix blackcurrant extract, chokeberry extract, bilberry extract, maqui berry extract, and blueberry extract, and add appropriate excipients such as calcium bicarbonate and pregelatinized starch to prepare tablets. The preparation method includes unpacking the excipients, cleaning and disinfecting, sieving, mixing with blackcurrant extract, chokeberry extract, bilberry extract, maqui berry extract, and blueberry extract, adding lubricant, checking tablet pressing, and drying to obtain tablets.

[0110] Biological test examples

[0111] Biological test example 1: Blue light damage experiment on mouse eyes

[0112] 1.1 Construction of blue light damage model for mouse eyes

[0113] (1) After 1 week of adaptive feeding of mice, randomly divide them into groups (n = 6): normal control group, irradiation control group, solvent control group, Example 1, Example 2, Example 3, and Example 4. Mice in Example 1, Example 2, Example 3, and Example 4 were intragastrically administered the 2376 composition with corresponding concentrations, and the solvent control group was treated with an equal volume of sunflower oil instead. Intragastric administration was continued for 45 days.

[0114] (2) Place the mice in a dark room for dark adaptation for 24 h. Except for the normal control group, the other groups were placed in a light damage device for light exposure (light damage working parameters: LED blue light strip wavelength is 452 nm, light intensity is 1400 - 1500 lux, and light exposure time is 1.5 h).

[0115] (3) After the light exposure, return the mice to the dark room for continued feeding. After 24 h, sacrifice the mice and take the mouse eyeballs for subsequent detection.

[0116] 1.1.2 Changes in retinal structure and cell morphology of mice

[0117] After removing the mouse eyeballs, prepare paraffin sections and observe by HE staining. Compare the retinal structure levels and cell morphology of mice in each group. Measure the changes in the retinal structure after HE staining of the paraffin sections under a 40×10 optical microscope, and focus on observing the thickness of the outer nuclear layer (ONL) of the retina after HE staining. The measurement position is the deepest part of the retinal damage.

[0118] 1.1.3 Detection of antioxidant indicators

[0119] Select related oxidative stress enzymes that characterize blue light damage and relief of visual fatigue, malondialdehyde (MDA), superoxide dismutase (SOD), and glutathione peroxidase (GSH-Px) to respectively characterize the degree of oxidative stress, antioxidant capacity, and the body's self-protection ability. Refer to the kit instructions to measure the contents of MDA, SOD, and GSH-Px in the serum of mice in each group to characterize their preventive and protective effects on oxidative stress damage.

[0120] MDA refers to malondialdehyde, which is a metabolite in living organisms. Under oxidative stress (such as blue light stimulation), lipids in cells will be oxidized, producing a large amount of MDA, thus increasing the degree of oxidative stress in cells. Therefore, the MDA oxidative stress index is an index that reflects the degree of oxidative stress in cells by measuring the content of MDA in cells. The higher the MDA, the stronger the oxidative stimulation received by tissue cells.

[0121] SOD is superoxide dismutase, which has strong antioxidant activity in the body. It can inhibit the synthesis and release of inflammatory factors such as histamine, 5-hydroxytryptamine, prostaglandin, and leukotriene, and has good health care effects such as anti-aging, anti-inflammatory, anti-radiation, and anti-fatigue.

[0122] GSH-Px is one of the main enzymes that catalyze the oxidation of reduced glutathione (GSH) in the glutathione redox cycle. GSH-Px can not only specifically catalyze the reaction of reduced glutathione with ROS to generate oxidized glutathione (GSSG), thereby protecting biological membranes from damage by ROS and maintaining the normal function of cells; but also has the abilities of protecting the liver, enhancing the body's immunity, antagonizing the damage of harmful metal ions to the body, and increasing the body's anti-radiation ability. The higher the GSH-Px, the stronger the anti-radiation ability on the surface of biological membranes.

[0123] 1.2 Test Results

[0124] 1.2.1 Changes in Retinal Structure and Cell Morphology

[0125] Observation by HE staining (see attachment Figure 1 ). In the blank control group of mice, the retinal structure was clearly layered, and the inner and outer segments of the retina were arranged neatly. Approximately 12-15 layers of clearly and closely arranged outer nuclear layers could be seen, with uniform staining and regular morphology; the boundaries of other layers of the retina were clear. In the blue light control group (light stimulation control) and the PBS solvent control group of mice, the inner and outer segments of the retina were arranged disorderly, the thickness of the outer nuclear layer of the retina was significantly thinned, the number of cell layers of the nucleus was only 5-7 layers, and the nuclei were darkly stained, shrunk, and the cell arrangement was disorderly; the boundary between the outer nuclear layer and the outer plexiform layer was unclear, and darkly stained nuclei appeared in the ganglion cell layer (see attachment Figure 1 ).

[0126] Compared with the model control group in Example 1 and Example 3, the retinal structure was arranged more neatly, the thickness of the outer nuclear layer was significantly increased, and the number of cell layers of the nucleus was approximately 6-11 layers. The number of darkly stained nuclei appearing in the ganglion cell layer was less (see attachment Figure 1 ). The ONL thickness of Example 1 (low dose of extract) and Example 3 (high dose of extract) was also significantly increased compared with the control group (see attachment Figure 2), where A is the light stimulation control group, B is the PBS control group, C is Example 1, D is Example 3, and E is the blank control group.

[0127] 1.2.2 Content and activity of key enzymes of oxidative stress in mouse eye tissue homogenate

[0128] The measurement results (average content) of MDA, SOD, and GSH-Px in the eyes of mice in each group are shown in Table 1.

[0129] The MDA content in the eyes of mice in the blue light control group and the solvent control group was significantly increased compared with the blank control group under blue light stimulation. As can be seen from Table 1, oral administration of Example 1, Example 2, Example 3, and Example 4 can reduce the damage to eye cells caused by blue light, etc.

[0130] The SOD content in the eyes of mice in the blue light control group and the solvent control group was significantly decreased under blue light stimulation. As can be seen from Table 1, oral administration of Example 1, Example 2, Example 3, and Example 4 can improve the anti-fatigue ability of eye cells.

[0131] The GSH-Px content in the eyes of mice in the blue light control group and the solvent control group was significantly decreased under blue light stimulation. As can be seen from Table 1, oral administration of Example 1, Example 2, Example 3, and Example 4 can improve the anti-damage ability of eye cells.

[0132] Table 1

[0133]

[0134] Biological test example 2 Experiment on the expression of Nrf2 protein in mouse eyes

[0135] After continuously administering the compound of the present invention to mice for 45 days and subjecting them to eye blue light damage stimulation, the eyeballs were taken for standby. The eyeballs of mice in the blank control group, the blue light irradiation model group, and Example 3 were selected for retinal and choroidal separation, and the expression of Nrf2 protein in the eyeballs of each group of mice was analyzed by immunofluorescence and Western Blot.

[0136] When autophagy is formed, cytoplasmic LC3-I is transformed into membrane-bound LC3-II. p62 is a widely studied autophagy substrate. The soluble p62 protein in Example 3 decreased, while the conversion of LC3-I to LC3-II increased, indicating the activation of autophagy. Heme Oxygenase-1 (HO-1) is an important antioxidant enzyme in the body, and HO-1 in Example 3 increased. NAD(P)H quinone dehydrogenase 1 (NQO1) is an Nrf2 target enzyme with a wide range of cell protection functions, and NQO-1 in Example 3 increased significantly. The results show that the compound of the present invention has good cell protection function.

[0137] The results show that the composition of the present invention has a cell protection function, and has a protective effect on the prevention of oxidative stress damage to the eye and a reparative effect on the retina.

[0138] Those skilled in the art of the present technology can understand that, unless otherwise defined, all terms (including technical terms and scientific terms) used herein have the same meaning as the general understanding of those of ordinary skill in the field to which the present invention belongs. It should also be understood that terms such as those defined in a general dictionary should be understood to have a meaning consistent with the meaning in the context of the prior art, and will not be interpreted with an idealized or overly formal meaning unless specifically defined as here.

[0139] It should be understood that the detailed description of the technical solutions of the present invention by means of the preferred embodiments above is illustrative rather than restrictive. Those of ordinary skill in the art can modify the technical solutions recorded in each embodiment on the basis of reading the specification of the present invention, or perform equivalent substitution on some of the technical features; and these modifications or substitutions do not make the essence of the corresponding technical solutions deviate from the spirit and scope of the technical solutions of each embodiment of the present invention.

Claims

1. A berry extract composition containing anthocyanins, wherein the anthocyanins are derived from one or more of blackcurrant extract, chokeberry extract, bilberry extract, maqui berry extract, blueberry extract, acai berry extract, and blackberry extract.

2. The berry extract composition according to claim 1, characterized in that The berry extract is a composition of blackcurrant extract, chokeberry extract, bilberry extract and maqui berry extract.

3. The berry extract composition according to claim 1, characterized in that The berry extract is a composition of blackcurrant extract, chokeberry extract, bilberry extract and maqui berry extract.

4. The berry extract composition according to claim 1 or 2, characterized in that The content of the blackcurrant extract is ≥1%.

5. The berry extract composition according to claim 1 or 2, characterized in that The content of the aronia berry extract is ≥10%.

6. The berry extract composition according to claim 1 or 2, characterized in that The content of the maqui berry extract is ≥1%.

7. The berry extract composition according to claim 1 or 2, characterized in that The content ratio of the blackcurrant extract to the maqui berry extract is 9:1 to 1:

9.

8. The berry extract composition according to claim 4, characterized in that The effective components of the blackcurrant extract are one or more of delphinidin-3-O-glucoside, delphinidin-3-O-rutinoside, cyanidin-3-O-glucoside and cyanidin-3-O-rutinoside.

9. The berry extract composition according to claim 5, characterized in that The effective components of the aronia berry extract are one or more of cyanidin-3-O-galactoside, cyanidin-3-O-glucoside, cyanidin-3-O-arabinoside and cyanidin-3-xyloside.

10. The berry extract composition according to claim 6, characterized in that The effective ingredients of the maqui berry extract are one or more of delphinidin-3-sambu-glucoside, delphinidin-3,5-diglucoside, cyanidin-3-sambubioside-5-glucoside, cyanidin diglucoside, delphinidin-3-sambubioside, delphinidin-3-glucoside, chlorocyanidin-3-sambubioside and cyanidin-3-O-glucoside.

11. The berry extract composition according to claim 1 or 2, characterized in that The effective components of the berry extract composition at least include delphinidin-3-O-glucoside, and the content of delphinidin-3-O-glucoside in the composition is ≥1%.

12. The berry extract composition according to claim 1 or 2, characterized in that The effective ingredients of the berry extract composition include at least one or more of delphinidin-3-glucoside, cyanidin-3-O-glucoside, delphinidin-3-sambu-glucoside and delphinidin-3,5-diglucoside.

13. The berry extract composition according to claim 12, characterized in that The berry extract composition has a delphinidin-3-sambuco-glucoside content of ≥1%.

14. The berry extract composition according to claim 12, characterized in that The berry extract composition has a delphinidin-3,5-diglucoside content of ≥1%.

15. The berry extract composition according to any one of claims 1 to 13, characterized in that The composition is added with auxiliary materials to prepare one or more of tablets, soft capsules, capsules, powders, oral liquids, sprays, solid beverages, crystal balls, soft candies, jellies, and popping candies.

16. The berry extract composition according to any one of claims 1 to 14, wherein the berry extract composition is used for protecting eyes, relieving eye fatigue, dry eyes, repairing retina, protecting eyesight, preventing and controlling myopia, and improving eye blood circulation.

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